A Chinese medicine composition with dual effects of anti-fatigue and intelligence-enhancing, and its preparation method and application

Abstract

A Chinese medicine composition with dual effects of anti-fatigue and intelligence-enhancing, and its preparation method and application. The present invention belongs to the field of Chinese medicine compositions. The preparation method of the Chinese medicine composition of the present invention is as follows: first, ginseng is crushed and sieved to obtain ginseng powder; then, angelica, Poria cocos, and Ziziphus jujuba seeds are mixed and decocted with water for multiple times, and the filtrate is concentrated under reduced pressure, dried, and crushed to obtain a mixed extract; finally, the ginseng powder and the mixed extract are mixed to obtain a Chinese medicine composition. The present invention takes the "inheritance-development-innovation" of traditional Chinese medicine and health as the idea, takes "emphasis on research and development, quality, and adaptability" as the core, and carries out scientific and reasonable compatibility and formulation, and investigates the preparation process and pharmacodynamics of the formulation. The obtained Chinese medicine composition not only replenishes the congenital "kidney essence", but also replenishes the acquired "spleen and stomach", which can achieve the function of overall regulation of the body, and improve the sub-health state of the body from the "fundamental", and the preparation process of the present invention is easy to industrialize.

Description

Technical Field

[0001] The invention belongs to the field of Chinese medicine compositions, and specifically relates to a Chinese medicine composition with dual effects of anti-fatigue and intelligence-enhancing, and a preparation method and application thereof. Background Art

[0002] Due to long-term, high-intensity, monotonous and repetitive learning, many teenagers have learning fatigue, accompanied by slow reaction and memory loss. Most of them are in sub-health status, which will seriously affect the physical and mental health of teenagers in the long run. However, at present, there is no better external intervention method except for teenagers to alleviate and improve through reasonable learning planning, work-rest combination and adjustment of mentality. As a great treasure of the Chinese nation, traditional Chinese medicine has the advantages and characteristics of early intervention, overall regulation and homeostasis maintenance. It has outstanding advantages in preventing and regulating sub-health status, which has been proven by thousands of years of clinical practice. Therefore, combined with the characteristics of sub-health of Chinese adolescents and the characteristics of traditional Chinese medicine, it is imperative to use traditional Chinese medicine as the main means to regulate the sub-health status of adolescent learning fatigue and memory loss. Summary of the invention

[0003] The present invention aims to provide a Chinese medicine composition having both anti-fatigue and intelligence-enhancing effects, and a preparation method and application thereof.

[0004] The purpose of the present invention is achieved through the following technical solutions:

[0005] One of the purposes of the present invention is to provide a Chinese medicine composition having both anti-fatigue and intelligence-enhancing effects, wherein the Chinese medicine composition is prepared from the following components in parts by weight:

[0006] 1-3 portions of ginseng;

[0007] 2-6 portions of Chinese Angelica;

[0008] Poria 3-6.5 parts;

[0009] 4-8 portions of Chinese date seeds.

[0010] It is further defined that the Chinese medicine composition is made of the following components in parts by weight: 1.5 parts of ginseng; 3 parts of angelica; 5 parts of Poria; and 5 parts of Ziziphus jujuba seeds.

[0011] The second object of the present invention is to provide a method for preparing a Chinese medicine composition having both anti-fatigue and intelligence-enhancing effects, wherein the preparation method is carried out according to the following steps:

[0012] S1: crushing ginseng, sieving, and obtaining ginseng powder;

[0013] S2: Mix angelica sinensis, Poria cocos and Ziziphus jujuba seeds, add water and boil for several times, and concentrate the filtrate under reduced pressure, dry and grind to obtain a mixed extract;

[0014] S3: Mixing the ginseng powder and the mixed extract to obtain a traditional Chinese medicine composition.

[0015] It is further defined that S1 passes through a 80-120 mesh sieve.

[0016] It is further defined that the material-to-liquid ratio in the decoction in S2 is 1:(6-12).

[0017] It is further defined that after being decocted in S2, the decocted product is filtered through a filter screen having 100 to 300 meshes.

[0018] It is further defined that S2 is decocted 1 to 3 times, each time for 0.5 to 2 hours.

[0019] It is further defined that S2 is concentrated under reduced pressure to a relative density of 1.22 to 1.45 and the concentration temperature is 60 to 80°C.

[0020] It is further defined that the drying temperature in S2 is 50-60°C.

[0021] The third object of the present invention is to provide a Chinese medicine composition having both anti-fatigue and intelligence-enhancing dual effects for preparing granules, tablets, capsules, pills, oral liquids and beverages.

[0022] The pharmacological effects of the present invention are as follows:

[0023] The present invention is guided by the theory of traditional Chinese medicine, takes qi and blood supplementing and treating both the symptoms and the root causes as the principle, and carries out scientific and reasonable compatibility and prescription. In the prescription, ginseng is the main medicine, which greatly supplements the vital energy, mainly supplements the five internal organs, calms the spirit, stabilizes the soul, makes the heart happy and improves intelligence, and takes it for a long time, lightens the body and prolongs life. The minister uses angelica to supplement blood and nourish the heart, and is compatible with the main medicine to supplement the deficiency of qi and blood, and has the effect of supplementing qi and blood. Poria and spiny jujube seeds are used to nourish the heart and calm the mind, so as to treat restlessness, and ginseng is used to strengthen the spleen and replenish qi, produce body fluid and calm the mind, and support the weak, and sincerely support the method of eliminating evil. All medicines are used together to play the merits of replenishing qi and nourishing blood, calming the mind and improving intelligence.

[0024] The pharmacology of each component is as follows:

[0025] Ginseng is the dried root and rhizome of Panaxginseng CAMeyer., a plant of the Araliaceae family. It has the effects of greatly replenishing vital energy, restoring pulse and strengthening deficiency, replenishing spleen and lung, promoting body fluid, and calming the mind. It is mainly used to treat physical weakness and weakness, spleen deficiency and poor appetite, lung deficiency and cough, long-term illness, palpitations and insomnia. "Shennong's Herbal Classic" says: It tastes sweet and is slightly cold. It mainly replenishes the five internal organs, calms the spirit, stabilizes the soul, stops palpitations, removes evil spirits, improves eyesight, makes people happy and improves intelligence. Long-term use can make the body lighter and prolong life. Modern pharmacological studies have shown that ginseng can play the role of greatly replenishing vital energy, replenishing spleen and lung, calming the mind and improving intelligence by regulating the central nervous system, immune function, endocrine function, and material metabolism.

[0026] Angelica sinensis is the dried root of Angelica sinensis (Oliv.) Diels, a plant of the Umbelliferae family. It is sweet, pungent and warm in taste. It is widely used in various clinical departments and is known as "Nine out of Ten Prescriptions are Angelica". It has the effects of nourishing blood and activating blood circulation, regulating menstruation and relieving pain, and moistening the intestines and relieving constipation. It is mainly used to treat sallow complexion due to blood deficiency, abdominal pain due to deficiency and cold, dizziness and palpitations. The "Collected Notes on the Classic of Materia Medica" says: it nourishes the five internal organs and builds muscle. Li Zhongzi said: Angelica sinensis is a blood medicine. The heart governs blood, the liver stores blood, and the spleen wraps blood, so it is included in it. Modern pharmacological studies have shown that Angelica sinensis has the effects of promoting hematopoietic function, improving blood rheology, dilating blood vessels and lowering blood pressure, resisting myocardial ischemia, and regulating endocrine hormones.

[0027] Poria cocos is the dried sclerotium of the Polyporaceae fungus Poria cocos (Schw.) Wolf. It tastes sweet, light and flat. It has the effects of infiltration and dampness, strengthening the spleen, calming the nerves and tranquilizing the mind. It is used for phlegm and fluid, palpitations, spleen deficiency, poor appetite, restlessness, palpitations and insomnia. "Shennong's Herbal Classic" says: Long-term use can calm the soul and nourish the mind, and it will not be hungry and prolong life. Ye Tianshi said: The spleen is the foundation of the acquired constitution. If the spleen is healthy, there will be no hunger, and if the qi is sufficient, life will be prolonged. Modern pharmacological studies have shown that Poria cocos has diuretic, sedative, immunomodulatory, liver-protecting and other effects, and has a good protective effect on the central nervous system, immune system, digestive system, etc.

[0028] The dried mature seeds of the rhamnaceae plant Ziziphus jujuba Mill. var. spinosa (Bunge) Hu ex H. F. Chou are sweet, sour and flat in nature and flavor. They nourish the heart and liver, calm the mind and soothe the nerves, relieve insomnia, palpitations and dreams, sweating due to physical weakness, and thirst due to loss of body fluids. They are mainly used to treat heart deficiency, loss of consciousness, fear and alarm, frequent trance, easy forgetfulness, restless sleep, dreams involving danger, and all heart diseases. Modern pharmacological studies have shown that the seeds of the Chinese jujube have the effects of sedation and hypnosis, improvement of learning and memory, brain protection, and enhancement of immunity.

[0029] Compared with the prior art, the present invention has the following significant effects:

[0030] (1) The present invention is guided by the theory of traditional Chinese medicine, based on the principles of replenishing qi and blood and treating both the symptoms and the root causes, and has carried out scientific and reasonable compatibility and formulation, and has studied the preparation process and pharmacodynamics of the formulation. Among them, ginseng is the main medicine, which greatly replenishes the vital energy, mainly replenishes the five internal organs, calms the spirit, stabilizes the soul, makes people happy and improves intelligence, and takes it for a long time, which makes the body light and prolongs life. The minister uses angelica to replenish blood and nourish the heart, and is compatible with the main medicine to replenish the deficiency of qi and blood, and has the effect of replenishing qi and blood. Poria cocos and spiny jujube seeds are used to nourish the heart and calm the mind, to treat restlessness, and ginseng is used to strengthen the spleen and replenish qi, promote the production of body fluid and calm the mind, and to support the weak, and to sincerely support the body to eliminate evil. All the medicines are used together to play the role of replenishing qi and nourishing blood, calming the mind and improving intelligence.

[0031] (2) The present invention replenishes both the innate "kidney essence" and the acquired "spleen and stomach", which can achieve the function of overall regulation of the body and fundamentally improve the sub-health state of the body. In addition, the cost of the traditional Chinese medicine used in the present invention is relatively low, and the preparation process is easy to industrialize. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1 The present invention is a flow chart of the preparation process of the application product of the Chinese medicine composition;

[0033] Figure 2 This is a comparison chart of the effects of each group of Chinese medicine compositions on the weight-bearing swimming time of mice;

[0034] Figure 3A This is a comparison chart of the effects of each group of Chinese medicine compositions on blood lactate in mice;

[0035] Figure 3B This is a comparison chart of the effects of each group of Chinese medicine compositions on mouse liver glycogen;

[0036] Figure 3C This is a comparison chart of the effects of each group of Chinese medicine compositions on the serum urea nitrogen content of mice;

[0037] Figure 4A This is a comparison chart of the effects of each group of Chinese medicine compositions on the latency period of platform jumping training in mice during platform jumping training;

[0038] Figure 4B This is a comparison chart of the effects of each group of Chinese medicine compositions on the latency of the mouse platform jumping test after the platform jumping training;

[0039] Figure 5A This is a comparison chart of the effects of each group of Chinese medicine compositions on the number of errors in the platform jumping training of mice during the platform jumping training;

[0040] Figure 5B This is a comparison chart of the effects of each group of Chinese medicine compositions on the number of errors in the platform jumping test of mice after the platform jumping training;

[0041] Fig. 6A This is a comparison chart of the effects of each group of Chinese medicine compositions on the dark avoidance latency of mice during dark avoidance training;

[0042] Figure 6B This is a comparison chart of the effects of each group of Chinese medicine compositions on the dark avoidance latency of mice after the dark avoidance training;

[0043] Fig. 7A This is a comparison chart of the effects of each group of Chinese medicine compositions on the number of dark avoidance errors of mice during dark avoidance training;

[0044] Figure 7B This is a comparison chart of the effects of each group of Chinese medicine compositions on the number of dark avoidance errors of mice after the dark avoidance training;

[0045] Fig. 8A This is a comparison chart of the effects of each group of Chinese medicine compositions on the average passive avoidance time of mice during shuttle training;

[0046] Figure 8B This is a comparison chart of the effects of each group of Chinese medicine compositions on the average passive avoidance time of mice after the shuttle training. DETAILED DESCRIPTION

[0047] In order to make the purpose, technical solution and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0048] The experimental methods used in the following examples are conventional methods unless otherwise specified. The materials, reagents, methods and instruments used are conventional materials, reagents, methods and instruments in the art unless otherwise specified, and can be obtained through commercial channels by those skilled in the art.

[0049] The terms "comprising," "including," "having," "containing," or any other variations thereof, as used in the following examples, are intended to cover a non-exclusive inclusion. For example, a composition, step, method, article, or apparatus comprising the listed elements is not necessarily limited to only those elements, but may include other elements not expressly listed or inherent to such composition, step, method, article, or apparatus.

[0050] When equivalent, concentration or other value or parameter is represented by the range limited by range, preferred range or a series of upper preferred value and lower preferred value, this should be understood as specifically disclosing all ranges formed by any pairing of any upper range limit or preferred value and any lower range limit or preferred value, regardless of whether the scope is disclosed separately. For example, when disclosing range "1 to 5", described range should be interpreted as including range "1 to 4", "1 to 3", "1 to 2", "1 to 2 and 4 to 5", "1 to 3 and 5" etc. When numerical range is described in this article, unless otherwise stated, the scope is intended to include its end value and all integers and fractions within the scope. In the present application specification and claims, range limitation can be combined and / or interchanged, if these ranges are not otherwise stated, include all sub-ranges contained therein.

[0051] The indefinite articles "a" and "an" before the elements or components of the present invention have no limitation on the quantity requirements (i.e. the number of occurrences) of the elements or components. Therefore, "a" or "an" should be interpreted as including one or at least one, and the elements or components in the singular form also include the plural form, unless the quantity obviously refers to the singular form only.

[0052] The endpoints and any values ​​of the ranges disclosed in the invention are not limited to the precise ranges or values, and these ranges or values ​​should be understood to include values ​​close to these ranges or values. For numerical ranges, the endpoint values ​​of each range, the endpoint values ​​of each range and the individual point values, and the individual point values ​​can be combined with each other to obtain one or more new numerical ranges, which should be regarded as specifically disclosed in this article.

[0053] Embodiment 1:

[0054] The preparation method of the Chinese medicine composition with both anti-fatigue and intelligence-enhancing dual effects of this embodiment is carried out by the following steps:

[0055] S1: Grind ginseng, pass it through a 100-mesh sieve, and weigh 15 g to obtain ginseng powder;

[0056] S2: Mix 30g of Angelica sinensis, 50g of Poria cocos, and 50g of Semen Ziziphi Spinosae, add 1040mL of water and boil for 3 times, each time for 1h, filter with a 200-mesh filter after boiling, combine the filtrates, and then concentrate under reduced pressure at 70°C to a relative density of 1.3, and then dry under reduced pressure at 60°C, finally crush the dry paste, and pass through a 100-mesh sieve to obtain a mixed extract;

[0057] S3: Mix the ginseng powder and the mixed extract to obtain a Chinese medicine composition.

[0058] Comparative Example 1:

[0059] The preparation method of the Chinese medicine composition of this comparative example is carried out according to the following steps:

[0060] Mix 15g of ginseng, 30g of angelica, 50g of Poria cocos, and 50g of Ziziphus jujuba seeds, add 1160mL of water and boil for 3 times, each time for 1 hour. After boiling, filter with a 200-mesh filter, combine the filtrates, and then concentrate under reduced pressure at 70°C to a relative density of 1.3, and then dry under reduced pressure at 60°C. Finally, crush the dry paste and pass through a 100-mesh sieve to obtain a Chinese medicine composition.

[0061] Comparative Example 2:

[0062] The preparation method of the Chinese medicine composition of this comparative example is carried out according to the following steps:

[0063] Ginseng, angelica, Poria cocos and spiny jujube seeds were crushed and passed through a 100-mesh sieve. 15 g of ginseng, 30 g of angelica, 50 g of Poria cocos and 50 g of spiny jujube seeds were weighed respectively and mixed to obtain a traditional Chinese medicine composition.

[0064] 1. Experiment on the anti-fatigue effect of Chinese medicine combination

[0065] 1.1 Experimental Materials

[0066] Ginseng (220901), Angelica sinensis (211101), Poria cocos (Y2206025), and Ziziphus jujuba seeds (fried) (2112001) were purchased from Jilin Pharmacy in Changchun, Jilin Province; liver / muscle glycogen determination kit, lactate (LD) kit (colorimetric method), and urea nitrogen (BUN) test kit (urease method) were all purchased from Nanjing Jiancheng Biotechnology Research Institute; SpectraMax i3 microplate reader (Molecular Devices, USA); Milli-Q Gradient A10 ultrapure water system (Millipore, USA).

[0067] 1.2 Experimental methods

[0068] 1.2.1 Animal grouping and determination of dosing

[0069] The mice were randomly divided into 7 groups, 10 mice in each group, namely the control group (Ctrl), the high-dose group (BG) of Example 1, the low-dose group (BD) of Example 1, the high-dose group (QG) of Comparative Example 1, the low-dose group (QD) of Comparative Example 1, the high-dose group (FG) of Comparative Example 2, and the low-dose group (FD) of Comparative Example 2. The control group was given an equal amount of normal saline, and each group was given the corresponding Chinese medicine composition. The dosage was calculated according to the crude drug, and the high-dose group of the three Chinese medicine compositions was given 18.85g / kg / d, and the low-dose group was given 9.425g / kg / d, and the administration was continued by gavage for 30 days.

[0070] 1.2.2 Weighted swimming test

[0071] 30 minutes after the last intragastric administration, 5% of the body weight of lead sheet was fixed to the tail of each group of mice, and they were placed in a 35 cm deep water tank for swimming, and the water temperature was controlled at about 25°C. The exhaustive swimming time of the mice was recorded from the time when the mice started to swim until their heads were not above the water for more than 10 seconds.

[0072] 1.2.3 Detection of liver glycogen, serum urea nitrogen and blood lactate content

[0073] (1) Determination of glycogen content: Mouse liver tissue was taken, rinsed with physiological saline, and dried with filter paper. 100 mg of liver tissue was weighed and operated according to the instructions of the glycogen kit.

[0074] (2) Determination of serum urea nitrogen content: Mouse plasma was obtained and reconstituted at room temperature, and the procedure was performed according to the instructions of the serum urea nitrogen (BUN) kit.

[0075] (3) Determination of blood lactate content: Collect whole blood from mice, reconstitute at room temperature, and follow the instructions of the blood lactate (LD) kit.

[0076] 1.3 Experimental Results

[0077] 1.3.1 Effects of each group of Chinese medicine compositions on the weight-bearing swimming time of mice

[0078] The effects of each group of Chinese medicine combination on the anti-fatigue weight-bearing swimming time of mice are shown in Figure 2 The results showed that the weighted swimming time of mice in the Chinese medicine combination groups of each embodiment was prolonged compared with the Ctrl group, among which the QG, QD, BG, BD and FG groups were significantly different from the Ctrl group (P<0.05), and the weighted swimming time of mice in the high concentration group was longer than that in the low concentration group, which was concentration-dependent. The above results show that the Chinese medicine combination of each group has the effect of relieving fatigue.

[0079] 1.3.2 Results of blood lactate, liver glycogen and serum urea nitrogen content tests

[0080] The effects of each group of Chinese medicine compositions on the blood lactate, glycogen and serum urea nitrogen content of mice are shown in Figure 3. The results show that compared with the Ctrl group, the blood lactate accumulation of mice in the Chinese medicine composition groups in each embodiment is reduced, showing a significant difference (P<0.05), and the effects are from strong to weak: Example 1> Comparative Example 1> Comparative Example 2, see Figure 3-A. Compared with the Ctrl group, the glycogen content of mice in the Chinese medicine composition groups in each embodiment is increased, and the increase effect of Example 1 is the strongest, showing a significant difference (P<0.05), see Figure 3-B. Compared with the Ctrl group, the serum urea nitrogen content of mice in the Chinese medicine composition groups in each embodiment has a downward trend, among which the serum urea nitrogen content of QG, BG, and FD groups compared with the Ctrl group is significantly different (P<0.05). The above results show that each group of Chinese medicine compositions has the effect of regulating blood lactate, glycogen and serum urea nitrogen in mice.

[0081] 2 Experiment on the intelligence-enhancing effect of Chinese herbal medicine combination

[0082] 2.1 Experimental Materials

[0083] Ginseng (220901), Chinese angelica (211101), Poria (Y2206025), and Ziziphus jujuba seeds (fried) (2112001) were purchased from Jilin Pharmacy, Changchun City, Jilin Province; (-)-scopolamine (Shanghai Aladdin Biochemical Technology Co., Ltd.); XR-XC105 shuttle box (Shanghai Xinruan Information Technology Co., Ltd.); Milli-Q GradientA10 ultrapure water system (Millipore, USA).

[0084] 2.2 Experimental methods

[0085] 2.2.1 Animal grouping and determination of dosing

[0086] The mice were randomly divided into 8 groups, each with 10 mice, namely the control group (Ctrl), the model group (M), the high-dose group (BG) of Example 1, the low-dose group (BD) of Example 1, the high-dose group (QG) of Comparative Example 1, the low-dose group (QD) of Comparative Example 1, the high-dose group (FG) of Comparative Example 2, and the low-dose group (FD) of Comparative Example 2. The blank group was given an equal amount of normal saline, and each administration group was given a corresponding Chinese medicine composition. The dosage was calculated according to the crude drug, the high-dose group was 18.85g / kg / d, the low-dose group was 9.425g / kg, and the administration was continued by gavage for 30 days.

[0087] 2.2.2 Establishment of memory impairment model

[0088] Scopolamine (0.5 mg / kg) was injected intraperitoneally into the model group and the drug-treated groups in each example 20 minutes before each behavioral experiment.

[0089] 2.2.3 Behavioral experiment - jumping platform experiment

[0090] 20 minutes after modeling, the mice were placed in a reaction box covered with a copper grid that could pass 36V electricity and the electricity was immediately turned on. The normal reaction of the mice was to jump onto the insulating platform in the reaction box to avoid harmful stimulation, and they might jump onto the copper grid again or multiple times. After being shocked, they quickly jumped back to the platform. This training was carried out for 3 minutes. The time of the first jump off the platform was recorded as the latent period. The number of times each mouse was shocked within 3 minutes was recorded as the number of errors. The number of mice shocked in each group was recorded, and the percentage of mice with incorrect reactions in each group was calculated (the number of mice shocked / 10). The test was started 24 hours later, and the latent period, the number of errors within 3 minutes, and the error reaction rate of each group were recorded as the test results.

[0091] 2.2.4 Behavioral experiment - dark avoidance experiment

[0092] After replicating the model of each modeling group for 20 minutes, each group of mice were placed in the bright room with their heads facing away from the hole to adapt to the environment for 5 minutes. After all the mice entered the dark room, the door connecting the bright room and the dark room was closed, and 36V electricity was supplied to the dark room for 30 seconds to make the mice suffer electric shock in the dark room. Formal training began 24 hours later. The mice were placed in the bright room with their heads facing away from the hole, and 36V electricity was supplied to the dark room. The time when the mice first entered the dark room was recorded as the dark avoidance latency period, and the number of times the mice entered the dark room and received electric shock within 3 minutes was recorded. The test began 24 hours later and the training was the same.

[0093] 2.2.5 Behavioral Experiment-Shuttle Experiment

[0094] Three days after the dark avoidance experiment, the shuttle experiment was conducted. The mice were placed on either side of the experimental box. Lights and beeps were presented 5 seconds later and lasted for 5 seconds. Then electrical stimulation was given for 10 seconds. If the mice ran to the opposite side when the lights and beeps were presented, it was called active avoidance. If they ran to the opposite side when they were electrically stimulated, it was called passive avoidance. After 20 minutes of modeling, the mice were placed in the box for adaptation training. The training was set to 10 times. The next day, learning training was conducted. The training was conducted 10 times. After 24 hours, the test was conducted. The training was set to 30 times. The average time of passive avoidance was recorded.

[0095] 2.3 Experimental Results

[0096] 2.3.1 Jumping platform test results

[0097] (1) Effects on the jumping latency of mice in each group

[0098] The effects of each group of Chinese medicine compositions on the latency of the platform jumping test are shown in Figure 4. Figure 4A It can be seen that there is no significant difference between the M group and the Ctrl group during the training period (P>0.05); Figure 4B It can be seen that there is a significant difference between the M group and the Ctrl group during the test period (P<0.05), indicating that the model was successfully established. In addition, there is a significant difference between the BG group and the M group (P<0.05).

[0099] (2) Effects on the number of jumping errors in each group of mice

[0100] The effects of each group of Chinese medicine compositions on the number of platform jumping errors are shown in Figure 5. Figure 5A It can be seen that the number of errors in the M group increased significantly compared with the Ctrl group (P<0.01), and the memory impairment model was successfully established. The number of errors in the QG, QD, BG, and FD groups decreased significantly compared with the M group (P<0.01). Figure 5B It can be seen that the average number of errors in group M is higher than that in group Ctrl, and the average number of errors in each group of Chinese medicine combination is lower than that in group M, which also shows that the prescription has an intelligence-enhancing effect.

[0101] (3) Effects on the error response rate of each group of mice in the platform jumping test

[0102] The effects of each group of Chinese medicine combination on the error response rate of the platform jumping are shown in Table 1. During training, the error response rate of each group was 100%. During the test, the error response rate of the M group was significantly different from that of the Ctrl group (P<0.01), the error response rate of the QD, BD, FG, and FD groups was significantly different from that of the M group (P<0.05), and the error response rate of the BG group was significantly different from that of the M group (P<0.01).

[0103] Table 1 Effects of each group of Chinese medicine compositions on the error response rate (n=10)

[0104]

[0105] 2.3.2 Dark avoidance test results

[0106] (1) Changes in latency of mice in each group in the dark avoidance test

[0107] The effects of each group of Chinese medicine compositions on the dark avoidance latency are shown in Figure 6. Fig. 6A It can be seen that there was no significant difference in the dark avoidance latency between the groups during training (P>0.05). Figure 6B It can be seen that the dark avoidance latency of the M group was significantly lower than that of the Ctrl group (P<0.05). Compared with the M group, the dark avoidance latency of each drug-treated group was significantly prolonged (P<0.01), indicating that each group of Chinese medicine compositions has the effect of prolonging the dark avoidance latency, indicating that it has a significant intelligence-enhancing effect.

[0108] (2) Changes in the number of dark avoidance errors of mice in each group

[0109] The effects of each group of Chinese medicine compositions on the number of dark avoidance errors are shown in Figure 7. Fig. 7A It can be seen that during the dark avoidance test training, the number of dark avoidance errors in the M group was significantly higher than that in the Ctrl group (P<0.01). Compared with the M group, the number of dark avoidance errors in the drug-treated groups of each embodiment was significantly reduced (P<0.05); Figure 7B It can be seen that when the dark avoidance test was performed, the number of dark avoidance errors in the M group was significantly higher than that in the Ctrl group (P<0.01), and the number of dark avoidance errors in the drug-administered groups of each embodiment was significantly lower than that in the M group (P<0.01). This indicates that each group of Chinese medicine compositions has the effect of reducing the number of dark avoidance errors, indicating that it has a significant intelligence-enhancing effect.

[0110] 2.3.3 Shuttle Experiment Results

[0111] The average time of passive avoidance of mice by each group of Chinese medicine composition is shown in Figure 8. Fig. 8A It can be seen that the average passive avoidance time of the M group during training was significantly higher than that of the Ctrl group (P<0.01). Compared with the M group, the average passive avoidance time of the mice in the drug-treated groups of each embodiment was significantly reduced (P<0.05). Figure 8B It can be seen that during the test, the average passive avoidance time of the M group was significantly higher than that of the Ctrl group (P<0.05). Compared with the M group, the average passive avoidance time of mice in each embodiment group was significantly reduced (P<0.05), indicating that the mice after drug administration were able to realize the impending electric shock more quickly.

[0112] Comparison of the overall efficacy of each group of Chinese medicine compositions using 3AHP analytic hierarchy process

[0113] 3.1 Construction of the overall drug efficacy comprehensive evaluation model

[0114] The overall drug efficacy comprehensive evaluation model was constructed based on the effective data set obtained from weighted swimming and behavioral experiments, see Table 2.

[0115] Table 2 Overall efficacy comprehensive evaluation model

[0116]

[0117] 3.2 AHP method to establish weight coefficients

[0118] According to the actual situation, the secondary indicators corresponding to anti-fatigue and intelligence training were quantified as weight indicators. The priority of the anti-fatigue experimental group was as follows: anti-fatigue swimming time > BUN content = LD content = glycogen content. The priority judgment matrix of the constructed pairwise comparison is shown in Table 3. The priority of the intelligence training group was as follows: the latent period of the platform jumping test = the latent period of the dark avoidance test = the number of errors in the dark avoidance test = the average time of passive avoidance in the shuttle test > the number of errors in the platform jumping training = the number of errors in the dark avoidance training = the average time of passive avoidance in the shuttle training. The priority judgment matrix of the constructed pairwise comparison is shown in Table 4.

[0119] Table 3 Anti-fatigue pair comparison priority judgment matrix

[0120]

[0121] Table 4 Puzzle pairwise comparison priority judgment matrix

[0122]

[0123] According to the matrix results in Table 3, the weight coefficients of anti-fatigue swimming time, BUN content, glycogen content, and LD content after hierarchical analysis are 0.4, 0.2, 0.2, and 0.2, respectively, and the consistency ratio factor (CR) = 0 < 0.1, that is, the indicator priority comparison judgment matrix has satisfactory consistency, and the obtained weight coefficient is valid; the matrix results in Table 4 are 0.092, 0.124, 0.152, 0.111, 0.221, 0.100, and 0.201, respectively, and the consistency ratio factor (CR) = 0.015 < 0.1, that is, the indicator priority comparison judgment matrix has satisfactory consistency, and the obtained weight coefficient is valid.

[0124] Each group was weighted separately to obtain the weighted average of each group. The results are shown in Tables 5 and 6.

[0125] Table 5 Anti-fatigue efficacy evaluation table

[0126]

[0127] Table 6 Evaluation table of drug efficacy of Yiqi

[0128]

[0129] The weighted average values ​​of the anti-fatigue and intelligence-enhancing experimental groups were summarized, and the efficacy of each Chinese medicine combination was comprehensively evaluated using the comprehensive weighted average value = anti-fatigue weighted average value * 0.5 + intelligence-enhancing weighted average value * 0.5. The results are shown in Table 7.

[0130] Table 7 Comprehensive evaluation table of drug efficacy

[0131]

[0132] Comprehensive comparison shows that each group of Chinese medicine compositions has the effects of anti-fatigue and intelligence-enhancing, and BG group>QG group>FG group, BD group>QD group>FD group, that is, the overall efficacy of Example 1 is better than that of Comparative Examples 1 and 2.

[0133] The above are only preferred specific embodiments of the present invention, which are all different implementations based on the overall concept of the present invention, and the protection scope of the present invention is not limited thereto. Any changes or substitutions that can be easily thought of by a person skilled in the art within the technical scope disclosed by the present invention should be included in the protection scope of the present invention. Therefore, the protection scope of the present invention should be based on the protection scope of the claims.

Claims

1. A Chinese medicine composition having both anti-fatigue and intelligence-enhancing effects, characterized in that: Made from the following components in parts by weight: 1~3 portions of ginseng; 2-6 portions of Chinese Angelica; Poria 3~6.5 parts; 4~8 portions of Chinese date seeds.

2. The Chinese medicine composition according to claim 1, characterized in that: The Chinese medicine composition is prepared from the following components in parts by weight: 1.5 parts of ginseng; 3 parts of angelica; 5 parts of tuckahoe; and 5 parts of spiny jujube seeds.

3. The method for preparing the Chinese medicine composition according to claim 1 or 2, characterized in that: Follow these steps: S1: crushing ginseng, sieving, and obtaining ginseng powder; S2: Mix angelica sinensis, Poria cocos and Ziziphus jujuba seeds, add water and boil for several times, and concentrate the filtrate under reduced pressure, dry and grind to obtain a mixed extract; S3: Mixing the ginseng powder and the mixed extract to obtain a traditional Chinese medicine composition.

4. The method according to claim 3, characterized in that S1 was passed through 80~120 mesh sieve.

5. The method according to claim 3, characterized in that: The material-liquid ratio of decoction in S2 is 1:(6~12).

6. The method according to claim 3, characterized in that After decocting in S2, filter through a 100-300 mesh filter.

7. The method according to claim 3, characterized in that S2 was decocted 1 to 3 times, each time for 0.5 to 2 hours.

8. The method according to claim 3, characterized in that S2 is concentrated under reduced pressure to a relative density of 1.22-1.45 and a concentration temperature of 60-80°C.

9. The method according to claim 3, characterized in that: The drying temperature in S2 is 50-60°C.

10. Use of the traditional Chinese medicine composition prepared by the method according to any one of claims 3 to 9 in the preparation of granules, tablets, capsules, pills and oral liquids.

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