Application of silkworm peptide, silkworm peptide liposome and whitening cream and preparation method thereof

Abstract

The application discloses application of a silkworm body peptide in preparation of whitening skin care products, and an amino acid sequence of the silkworm body peptide is shown as SEQ ID NO. 1. The silkworm body peptide is wrapped by liposomes, and is matched with a natural oil base, so that the silkworm body peptide can be stably stored in a skin care product system and is not affected by organic solvents and metal ions; the liposomes have a special bilayer structure, and have hydrophilicity and hydrophobicity, and are consistent with the main components of the skin; the silkworm body peptide is embedded in the liposomes, so that transdermal absorption of the components is promoted, and bioavailability is improved. The silkworm body peptide can reach 19.69% in tyrosinase inhibition rate after being diluted by 50 times, and can be used to prepare whitening skin care products with corresponding silkworm body peptide concentration.

Description

Technical Field

[0001] The present invention relates to the technical field of skin care products, in particular to the technical field of skin care products containing silkworm peptides. More particularly, the present invention relates to an application of silkworm peptides, silkworm peptide liposomes and a whitening cream and a preparation method thereof. Background Art

[0002] The poem "A face as white as cream, eyes as white as lacquer" captures the aesthetic aspirations of Asian women throughout history: smooth, flawless, and fair skin, a key claim of numerous skincare products. Modern science confirms that skin darkening, yellowing, freckles, and melasma are all linked to melanin accumulation. The mechanism of whitening is primarily through inhibiting or blocking melanin production pathways. Melanin production is primarily stimulated by ultraviolet light or inflammation, stimulating melanocytes in the basal layer of the epidermis. Tyrosinase, acting as a catalyst for the production of intracellular tyrosine, converts it into dopaminergic (eumelanin) and pheomelanin through various pathways. These melanins gradually rise to the stratum corneum, where they eventually aggregate and become visible.

[0003] As a model organism of the Lepidoptera order, the silkworm (Bombyx mori) possesses numerous pigmentation mutants, making it an ideal candidate for studying pigment metabolism and the mechanisms of markings. The OKA-type4 silkworm peptide, a peptide fragment accidentally obtained and modified during experiments, has been shown to effectively eliminate the black markings of quail-like mutant silkworms when injected into their body fluids, rendering them indistinguishable from normal silkworms.

[0004] Nowadays, more and more consumers prefer natural active ingredients with efficacy, such as anthocyanins, flavonoids, polyphenol extracts from plants, bioactive peptides, astaxanthin, chitosan from animals, etc. These substances have incomparable whitening and antioxidant properties. Compared with chemically synthesized whitening agents, they are safer, less sensitive, and have no toxic side effects.

[0005] This study applied OKA-type4 silkworm peptide to whitening skin care products and developed a cream product with whitening effect on human skin, which is of great significance to promoting the development and application of silkworm peptide. Summary of the Invention

[0006] One purpose of the present invention is to apply OKA-type4 silkworm peptide to skin care products, provide a silkworm peptide liposome that can encapsulate the silkworm peptide, and a silkworm peptide whitening cream skin care product with a whitening effect.

[0007] The purpose of the present invention is achieved through the following technical solutions:

[0008] The invention discloses an application of a silkworm peptide in the preparation of a whitening skin care product. The amino acid sequence of the silkworm peptide is shown in SEQ ID NO.1.

[0009] Preferably, in the application, the concentration of the silkworm body peptide in the whitening skin care product is 8 to 40 mg / mL.

[0010] Preferably, in the application, the concentration of the silkworm body peptide in the whitening skin care product is 20 mg / mL.

[0011] Preferably, in the application, the whitening skin care product is a cream, an essence or a facial mask.

[0012] A method for preparing silkworm peptide liposomes, comprising:

[0013] Dissolving the silkworm peptide according to claim 1 in PBS buffer to prepare an aqueous phase;

[0014] Soy lecithin, cholesterol and sodium cholate are added to anhydrous ethanol to prepare an organic phase;

[0015] The aqueous phase is added to the organic phase to obtain a mixture with a volume ratio of aqueous phase: organic phase = 1:1-2;

[0016] The mixture is ultrasonically treated and rotary evaporated to obtain a film, which is then dissolved in Tween-80 and PBS buffer, subjected to secondary rotary evaporation and ultrasonic treatment to obtain an emulsion, which is filtered and freeze-dried to obtain a powder, which is silkworm peptide liposome.

[0017] A silkworm peptide liposome is prepared by the preparation method.

[0018] A whitening cream comprising the following raw material components:

[0019] Oil phase base material: 3-4 portions of natural oil, 2-3 portions of emollient, 3 portions of emulsifier, and 3-4 portions of the silkworm peptide liposome;

[0020] Aqueous phase base: deionized water 18-22, soothing anti-allergic agent 2-3, plant extract 0.5-2, antioxidant 0.2-1.

[0021] Preferably, the whitening cream comprises 0.1-0.2 of a regulator, 2-3 of a preservative, and 0.2-1 of a flavor.

[0022] Preferably, the whitening cream comprises the following raw material components:

[0023] Oil phase base: natural oil 4, emollient 3, emulsifier 6, silkworm peptide liposome 4 according to claim 6;

[0024] Aqueous base: deionized water 18, soothing anti-allergic agent 3, plant extract 2, antioxidant 1;

[0025] Conditioner 0.2; Preservative 3; Flavor 1.

[0026] A method for preparing the whitening cream comprises:

[0027] Prepare the aqueous base: Heat the aqueous base to 40-50°C and add 0.5 parts of xanthan gum to thicken it while stirring at high speed to obtain an aqueous phase;

[0028] Prepare the oil phase base material, heat the oil phase base material to 70-80°C to melt it evenly to obtain the oil phase;

[0029] The oil phase is then mixed with the water phase, emulsified while stirring, and allowed to stand to cool; after cooling to 40°C, flavors, preservatives, and regulators are added, and high-speed homogenization is performed at 3000-4000 rpm / min for 40 minutes to obtain the product.

[0030] The present invention includes at least the following advantages and beneficial effects:

[0031] 1. The present invention uses liposomes to encapsulate silkworm peptides and is combined with natural oil base materials, so that the silkworm peptides can be stably stored in the skin care system and are not affected by organic solvents and metal ions; liposomes have a special bilayer structure, which is both hydrophilic and hydrophobic, and is consistent with the main composition of the skin. Embedding silkworm peptides in liposomes can promote the transdermal absorption of the ingredients and also improve the bioavailability.

[0032] 2. The silkworm peptide of the present invention can inhibit tyrosinase by 19.69% after being diluted 50 times, and can be made into whitening skin care products with corresponding silkworm peptide concentration to exert its effect.

[0033] 3. The whitening cream prepared by the silkworm peptide liposome of the present invention has been tested on hands. Compared with not applying, it can improve the moisture of the skin and reduce the oiliness; improve the fine lines of the skin and brighten the skin luster; it can significantly improve the pigmentation depth one hour after application and also has a good effect on shrinking pores.

[0034] 4. The silkworm peptide of the present invention was tested by applying it on the guinea pig epidermis. The non-administered group had an increase in melanocytes and melanin granules, and the epidermis showed hyperplasia. The stratum corneum and granular layer were thicker, inflammatory cells were found in the dermis, the tissue was loosely arranged, and there were a large number of stretched melanin granules. The inflammatory factors in the silkworm peptide-administered group were significantly reduced, and the epidermal hyperplasia was improved. The cells were arranged more tightly, and the number of melanocytes was reduced.

[0035] Other advantages, objectives and features of the present invention will be reflected in part from the following description and will be understood by those skilled in the art through study and practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] Figure 1 is the tyrosinase inhibition rate of the silkworm peptide of the present invention;

[0037] Figure 2 This is a picture of the guinea pig skin after the administration period of the model of the present invention;

[0038] Figure 3 This is the staining picture of the non-drug group in the HE staining experiment of the present invention;

[0039] Figure 4 This is the staining picture of the drug administration group in the HE staining experiment of the present invention;

[0040] Figure 5 This is a staining picture of the non-drug-treated group in the Masson experiment of the present invention;

[0041] Figure 6 This is a staining picture of the drug-dosing group in the Masson experiment of the present invention. DETAILED DESCRIPTION

[0042] The present invention is further described in detail below with reference to examples so that those skilled in the art can implement the invention with reference to the description.

[0043] It should be noted that the experimental methods described in the following embodiments are conventional methods unless otherwise specified, and the reagents and materials can be obtained from commercial channels unless otherwise specified.

[0044] The present invention is further described in detail below with reference to the embodiments so that those skilled in the art can implement the invention with reference to the description.

[0045] The natural oils used in the present invention are one or more of phytosterol, iso-16 alkyl ether, squalane, C16-C18 stearic acid glyceryl ester, polydimethylsiloxane and the like.

[0046] The emollient is one or more of butanetriol, propylene glycol, glycerin, etc.

[0047] The emulsifier is one or more of cetearyl alcohol, glyceryl stearate, sorbitan ester, Seppic 305, etc.

[0048] Soothing and anti-allergic agents include allantoin and the like.

[0049] The plant extract is chamomile extract and the like.

[0050] Antioxidants include vitamin C, etc.

[0051] The regulator is EDTA-2Na, triethanolamine, etc.

[0052] Preservatives include parahydroxyacetophenone, phenoxyethanol, pentylene glycol, etc.

[0053] The flavor is rose flavor and the like.

[0054] Example 1

[0055] Synthesis of OKA-type4 silkworm peptide

[0056] The OKA-type 4 silkworm body peptide was synthesized according to the amino acid sequence of the silkworm body peptide. The amino acid sequence is shown in SEQ ID NO. 1, specifically: EARHSGYLPYQMF, with 13 amino acids and a synthesis purity of 95%.

[0057] Example 2

[0058] Preparation of Silkworm Peptide Liposomes 1

[0059] The silkworm peptide liposomes were prepared by thin film dispersion method.

[0060] Preparation of aqueous phase: The silkworm body peptide prepared in Example 1 was dissolved in a PBS buffer solution at pH 4.7 to prepare an aqueous phase with a silkworm body peptide concentration of 0.2 g / ml.

[0061] Preparation of organic phase: 20 mg of soybean lecithin, 1 mg of cholesterol, and 1 mg of sodium cholate were added to 10 ml of anhydrous ethanol to prepare an organic phase.

[0062] Under stirring at 300-400 rpm / min, the aqueous phase was added dropwise to the organic phase in batches using a 1.5 mL needle to obtain a mixture with a volume ratio of aqueous phase:organic phase = 1:2.

[0063] The mixture was ultrasonically treated at 500W for 2-8 minutes for the first time, and then subjected to reduced pressure rotary evaporation at 37°C. After evaporation until a milky white film was formed, a small amount of Tween-80 and PBS buffer was added and shaken to dissolve the film.

[0064] After removing excess water by rotary evaporation at 37°C for the second time, ultrasonic treatment was performed at 500W for 2-4 minutes, and the formed emulsion was filtered through a 0.45 μm micropore filter.

[0065] The filtered emulsion is freeze-dried into powder to obtain silkworm peptide liposome powder, wherein the silkworm peptide content is about 4.5%.

[0066] Example 3

[0067] Preparation of Silkworm Peptide Liposome 2

[0068] The silkworm peptide liposomes were prepared by thin film dispersion method.

[0069] Preparation of aqueous phase: The silkworm peptide prepared in Example 1 was dissolved in a PBS buffer solution at pH 4.7 to prepare an aqueous phase with a silkworm peptide concentration of 0.4 g / ml.

[0070] Preparation of organic phase: 20 mg of soybean lecithin, 1 mg of cholesterol, and 1 mg of sodium cholate were added to 10 ml of anhydrous ethanol to prepare an organic phase.

[0071] Under stirring at 300-400 rpm / min, the aqueous phase was added dropwise to the organic phase in batches using a 1.5 mL needle to obtain a mixture with a volume ratio of aqueous phase:organic phase = 1:1.5.

[0072] The mixture was ultrasonically treated at 500W for 2-8 minutes for the first time, and then subjected to reduced pressure rotary evaporation at 37°C. After evaporation until a milky white film was formed, a small amount of Tween-80 and PBS buffer was added and shaken to dissolve the film.

[0073] After removing excess water by rotary evaporation at 37°C for the second time, ultrasonic treatment was performed at 500W for 2-4 minutes, and the formed emulsion was filtered through a 0.45 μm micropore filter.

[0074] The filtered emulsion is freeze-dried into powder to obtain silkworm peptide liposome powder, wherein the silkworm peptide content is about 12%.

[0075] Example 4

[0076] Preparation of Silkworm Peptide Liposomes 3

[0077] The silkworm peptide liposomes were prepared by thin film dispersion method.

[0078] Preparation of aqueous phase: The silkworm peptide prepared in Example 1 was dissolved in a PBS buffer solution at pH 4.7 to prepare an aqueous phase with a silkworm peptide concentration of 0.6 g / ml.

[0079] Preparation of organic phase: 20 mg of soybean lecithin, 1 mg of cholesterol, and 1 mg of sodium cholate were added to 10 ml of anhydrous ethanol to prepare an organic phase.

[0080] Under stirring at 300-400 rpm / min, the aqueous phase was added dropwise to the organic phase in batches using a 1.5 mL needle to obtain a mixture with a volume ratio of aqueous phase:organic phase = 1:1.

[0081] The mixture was ultrasonically treated at 500W for 2-8 minutes for the first time, and then subjected to reduced pressure rotary evaporation at 37°C. After evaporation until a milky white film was formed, a small amount of Tween-80 and PBS buffer was added and shaken to dissolve the film.

[0082] After removing excess water by rotary evaporation at 37°C for the second time, ultrasonic treatment was performed at 500W for 2-4 minutes, and the formed emulsion was filtered through a 0.45 μm micropore filter.

[0083] The filtered emulsion is freeze-dried into powder to obtain silkworm body peptide liposome powder, wherein the content of silkworm body peptide is about 21%.

[0084] Example 5

[0085] Preparation of Silkworm Body Peptide Whitening Cream 1

[0086] The formula of silkworm body peptide whitening cream is as follows, calculated by weight, including:

[0087] Oil phase base: natural oil 3%, emollient 2%, emulsifier 3, silkworm peptide liposome powder of Example 4 3%;

[0088] Aqueous base: deionized water 21, soothing anti-allergic agent 2, plant extract 0.5, antioxidant 0.2;

[0089] Conditioner 0.1; Preservative 2; Flavor 0.2.

[0090] During preparation:

[0091] Prepare a water phase base material, heat the water phase base material to 40-50°C, add 0.5 parts of xanthan gum to thicken it while stirring at high speed, and obtain a water phase.

[0092] Prepare the oil phase base material, heat the oil phase base material to 70-80°C to melt it evenly, and obtain the oil phase.

[0093] The oil phase is then mixed with the water phase, emulsified under high-speed stirring for 30 minutes, and allowed to cool. After cooling to 40°C, flavors, preservatives, and regulators are added, and high-speed homogenization is performed at 3000-4000 rpm / min for 40 minutes. After cooling to room temperature, it can be bottled.

[0094] Example 6

[0095] Preparation of Silkworm Body Peptide Whitening Cream 2

[0096] The formula of silkworm body peptide whitening cream is as follows, calculated by weight, including:

[0097] Oil phase base: natural oil 3.5, emollient 2.5, emulsifier 4.5, silkworm peptide liposome powder of Example 4 3.5;

[0098] Aqueous base: deionized water 22, soothing anti-allergic agent 2.5, plant extract 1.5, antioxidant 0.6;

[0099] Conditioner 0.15; Preservative 2.5; Flavor 0.6.

[0100] During preparation:

[0101] Prepare a water phase base material, heat the water phase base material to 40-50°C, add 0.5 parts of xanthan gum to thicken it while stirring at high speed, and obtain a water phase.

[0102] Prepare the oil phase base material, heat the oil phase base material to 70-80°C to melt it evenly, and obtain the oil phase.

[0103] The oil phase is then mixed with the water phase, emulsified under high-speed stirring for 30 minutes, and allowed to cool. After cooling to 40°C, flavors, preservatives, and regulators are added, and high-speed homogenization is performed at 3000-4000 rpm / min for 40 minutes. After cooling to room temperature, it can be bottled.

[0104] Example 7

[0105] Preparation of Silkworm Body Peptide Whitening Cream 3

[0106] The formula of silkworm body peptide whitening cream is as follows, calculated by weight, including:

[0107] Oil phase base: natural oil 4, emollient 3, emulsifier 6, silkworm peptide liposome powder 4 of Example 4;

[0108] Aqueous base: deionized water 21, soothing anti-allergic agent 3, plant extract 2, antioxidant 1;

[0109] Conditioner 0.2; Preservative 3; Flavor 1.

[0110] During preparation:

[0111] Prepare a water phase base material, heat the water phase base material to 40-50°C, add 0.5 parts of xanthan gum to thicken it while stirring at high speed, and obtain a water phase.

[0112] Prepare the oil phase base material, heat the oil phase base material to 70-80°C to melt it evenly, and obtain the oil phase.

[0113] The oil phase is then mixed with the water phase, emulsified under high-speed stirring for 30 minutes, and allowed to cool. After cooling to 40°C, flavors, preservatives, and regulators are added, and high-speed homogenization is performed at 3000-4000 rpm / min for 40 minutes. After cooling to room temperature, it can be bottled.

[0114] Effect experiment

[0115] 1. In vitro whitening experiment of silkworm peptide

[0116] After the silkworm peptide of Example 1 was diluted with PBS solution, the inhibition rate of silkworm peptide on tyrosinase was tested, and it was found that the silkworm peptide could effectively inhibit the production of melanin catalyzed by tyrosinase and had a certain whitening effect. Figure 1 As shown, when the silkworm body peptide is diluted 50 times, the tyrosinase inhibition rate can reach 19.69%. At this time, the whitening effect of whitening skin care products such as creams is the best. The proportion of silkworm body peptide in the creams prepared in Examples 5 to 7 is close to this range.

[0117] 2. Use of Silkworm Peptide Whitening Cream on Hands

[0118] Testing method: Apply one drop of the silkworm body peptide whitening cream of Example 7 (2*2 cm in size) on the back of the hand, and compare with no application.

[0119] Testing instrument: Magic Mirror Intelligent Skin Analyzer, Guangzhou Magic Beauty Instrument Equipment Co., Ltd.

[0120] Table 1 Effect of applying silkworm peptide whitening cream on hands

[0121]

[0122] From Table 1 we can see:

[0123] 1. After applying the cream, it improves the moisture of the skin and reduces oil production;

[0124] 2. After applying the cream, fine lines on the skin are significantly improved and the skin luster is brightened;

[0125] 3. The cream can effectively improve the depth of pigmentation one hour after application and also has a good effect on shrinking pores.

[0126] 3. Silkworm peptide application on guinea pig epidermis test

[0127] 1. Experimental Materials

[0128] Guinea pig, OKA-type 4 silkworm peptide of Example 1.

[0129] 2. Experimental instruments

[0130] Dehydrator (DIAPATH, Donatello), embedding machine (JB-P5, Junjie Electronics Co., Ltd., Wuhan), pathology slicer (RM2016, Leica Microsystems, Shanghai), freezing table (JB-L5, Junjie Electronics Co., Ltd., Wuhan), tissue spreader (KD-P, Kedi Instrument Equipment Co., Ltd., Jinhua City, Zhejiang Province), staining machine (DIAPATH, Giotto), oven (GFL-230, Laiborui Instrument Equipment Co., Ltd., Tianjin), slides (Servicebio, G6004), upright optical microscope (Nikon Eclipse E100, Nikon, Japan), imaging system (Nikon DS-U3, Nikon, Japan).

[0131] 3. Experimental Materials

[0132] Anhydrous ethanol (Sinopharm Chemical Reagent Co., Ltd., 100092683), xylene (Sinopharm Chemical Reagent Co., Ltd., 10023418), HE staining solution set (Servicebio, G1003), neutral gum (Sinopharm Chemical Reagent Co., Ltd., 10004160), Masson staining solution set (Servicebio, G1006).

[0133] 4. Modeling method

[0134] The light source was a 400W xenon lamp containing long-wave UVA and medium-wave UVB, and a 1000W metal halide lamp with a peak spectrum of 360nm, of which UVA accounted for 70% and UVB accounted for 4%. The guinea pigs were shaved on both sides of their backs, exposing an area of ​​approximately 3*3cm. The irradiation was then performed at a distance of 10cm from the skin, once a day for 7 consecutive days, for a total cumulative irradiation of 5000mJ / cm 2 The silkworm body peptide was dissolved in PBS solution and diluted to obtain a sample with a concentration of 20 mg / mL of silkworm body peptide. After modeling, the sample was applied to the area. The left side was not treated, and the right side was applied. The sample was applied once a day for one week as the dosing cycle. After the end of the dosing cycle, the skin tissue was taken for testing. The picture of the guinea pig skin after the end of the dosing cycle is as follows: Figure 2 shown.

[0135] 5. HE staining experimental method

[0136] The epidermal tissue was sliced ​​in sequence and placed in xylene I for 20 minutes, xylene II for 20 minutes, anhydrous ethanol I for 5 minutes, anhydrous ethanol II for 5 minutes, and 75% alcohol for 5 minutes, and then washed with tap water. The slices were stained with hematoxylin solution for 3-5 minutes, washed with tap water, differentiated with differentiation solution, washed with tap water, blued with bluing solution, and rinsed with running water. The slices were dehydrated in 85% and 95% gradient alcohol for 5 minutes each, and stained in eosin solution for 5 minutes. The slices were placed in anhydrous ethanol I for 5 minutes, anhydrous ethanol II for 5 minutes, anhydrous ethanol III for 5 minutes, xylene I for 5 minutes, and xylene II for 5 minutes to make them transparent, and then sealed with neutral gum. Microscopic examination and image acquisition and analysis were performed. The results of the non-medicated group are shown in Figure 2. Figure 3 The results of the drug-treated group are shown in Figure 4 shown.

[0137] 6. Masson experimental method

[0138] Dewax the paraffin sections to water: sequentially place the epidermal tissue sections in xylene I for 20 minutes, xylene II for 20 minutes, anhydrous ethanol I for 5 minutes, anhydrous ethanol II for 5 minutes, and 75% alcohol for 5 minutes, and then wash with tap water. Immerse the sections in Masson A solution overnight and wash with tap water. Place the sections in a dye solution mixed with Masson B solution and Masson C solution in equal proportions, immerse for 1 minute, wash with tap water, differentiate with 1% hydrochloric acid alcohol, and then wash with tap water. Place the sections in Masson D solution for 6 minutes and rinse with tap water. Immerse in Masson E solution for 1 minute. Without washing with water, drain slightly and directly place in Masson F solution for staining for 2-30 seconds. Rinse and differentiate with 1% glacial acetic acid and dehydrate with two cylinders of anhydrous ethanol. Place the sections in a third cylinder of anhydrous ethanol for 5 minutes, make them transparent with xylene for 5 minutes, and seal with neutral gum. Observe under a microscope and collect and analyze images. The pictures of the non-medicated group are as follows. Figure 5 As shown, the pictures of the drug-treated group are as follows Figure 6 shown.

[0139] Figure 2 These are the results of naked-eye observation of the guinea pig epidermis: the shaved area on the left side of the picture is the non-drug group, and the shaved area on the right side is the drug group. There is no significant difference between the non-drug group and the drug group. The irradiated area becomes rough and hard, loses elasticity, and shows symptoms of skin aging.

[0140] Figure 3 and Figure 4 、 Figure 5 and Figure 6 Epidermal tissue staining results:

[0141] In the non-medicated group, the number of melanocytes and melanin granules increased, the epidermis showed hyperplasia, the stratum corneum and granular layer were thicker, inflammatory cells were seen in the dermis, the tissue was loosely arranged, and there were a large number of stretched melanin granules.

[0142] In the group treated with silkworm body peptide, the inflammatory factors were significantly reduced, the epidermal hyperplasia was improved, the cells were arranged more closely, and the number of melanocytes was reduced.

[0143] Although the embodiments of the present invention have been disclosed above, they are not limited to the applications listed in the specification and embodiment. They can be applied to various fields suitable for the present invention. For those familiar with the art, additional modifications can be easily realized.

Claims

1. A use of a silkworm peptide in the preparation of a whitening skin care product, characterized in that: The amino acid sequence of the silkworm body peptide is shown in SEQ ID NO.

1.

2. The use according to claim 1, characterized in that The concentration of silkworm body peptide in whitening skin care products is 8~40mg / mL.

3. The use according to claim 1, characterized in that The concentration of silkworm body peptide in whitening skin care products is 20mg / mL.

4. The use according to claim 1 or 3, characterized in that The whitening skin care product is a cream, an essence or a facial mask.

5. A whitening cream, characterized in that: The raw material components are as follows, in parts by weight: Oil phase base: natural oil 3-4, softener 2-3, emulsifier 3, silkworm peptide liposome 3-4; Aqueous base: deionized water 18-22, soothing anti-allergic agent 2-3, plant extract 0.5-2, antioxidant 0.2-1; The preparation steps of silkworm peptide liposomes are as follows: Dissolving the silkworm peptide according to claim 1 in PBS buffer to prepare an aqueous phase; Soy lecithin, cholesterol and sodium cholate are added to anhydrous ethanol to prepare an organic phase; The aqueous phase was added to the organic phase to obtain a mixture with a volume ratio of aqueous phase: organic phase = 1: 1-2; The mixture is ultrasonically treated and rotary evaporated to obtain a film, which is then dissolved in Tween-80 and PBS buffer, subjected to secondary rotary evaporation and ultrasonic treatment to obtain an emulsion, which is filtered and freeze-dried to obtain a powder, which is silkworm peptide liposome.

6. The whitening cream according to claim 5, wherein It includes 0.1-0.2 of regulator, 2-3 of preservative and 0.2-1 of flavor.

7. A whitening cream, characterized in that: The raw material components are as follows, in parts by weight: Oil phase base: natural oil 4, emollient 3, emulsifier 6, silkworm peptide liposome 4; Aqueous base: deionized water 21, soothing anti-allergic agent 3, plant extract 2, antioxidant 1; Conditioner 0.2; Preservative 3; Flavor 1; During preparation: Prepare the aqueous phase base, heat the aqueous phase base to 40-50°C, add 0.5 parts of xanthan gum to thicken it while stirring at high speed, and obtain the aqueous phase; Prepare the oil phase base material, heat the oil phase base material to 70-80°C to melt it evenly to obtain the oil phase; The oil phase is then mixed with the water phase, emulsified under high-speed stirring for 30 minutes, and allowed to cool. After cooling to 40°C, flavors, preservatives, and conditioning agents are added, and high-speed homogenization is performed at 3000-4000 rpm / min for 40 minutes. After cooling to room temperature, the mixture is bottled. The method of preparing silkworm peptide liposomes by thin film dispersion method comprises: Prepare the aqueous phase: dissolve the silkworm peptide according to claim 1 in a PBS buffer solution with a pH of 4.7 to prepare an aqueous phase with a silkworm peptide concentration of 0.6 g / ml; Prepare the organic phase by adding 20 mg of soy lecithin, 1 mg of cholesterol, and 1 mg of sodium cholate to 10 ml of anhydrous ethanol. Under stirring at 300-400 rpm / min, the aqueous phase was added dropwise to the organic phase using a 1.5 mL needle to obtain a mixture with a volume ratio of aqueous phase: organic phase = 1:1; The mixture was first sonicated at 500W for 2-8 minutes, and then vacuum evaporated at 37°C until a milky white film was formed. Tween-80 and PBS buffer were then added and shaken to dissolve the film. After removing excess water by vacuum rotary evaporation at 37°C for the second time, ultrasonic treatment was performed at 500W for 2-4min, and the formed emulsion was filtered through a 0.45μm micropore filter; The filtered emulsion is freeze-dried into powder to obtain silkworm peptide liposome powder.

8. A method for preparing the whitening cream according to claim 6, characterized in that: include: Prepare the aqueous base: heat the aqueous base to 40-50°C and add xanthan gum to thicken it while stirring at high speed to obtain the aqueous phase; Prepare the oil phase base material, heat the oil phase base material to 70-80°C to melt it evenly to obtain the oil phase; The oil phase is then mixed with the water phase, emulsified while stirring, and allowed to stand to cool; after cooling to 40°C, flavors, preservatives, and regulators are added, and high-speed homogenization is performed at 3000-4000 rpm / min for 40 minutes to obtain the product.

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