A PCR eight-tube freeze-drying plate rack and its use method
By designing the PCR eight-connected pipe freeze-drying plate rack, the positioning and height-limiting arm structure of the base, pipe bracket and cover bracket are used to solve the problem of lax fixation and deformation of the pipe cover during the PCR eight-connected pipe lyophilization process, and a rapid and effective freeze-drying operation is achieved, reducing costs and shortening the production cycle.
Patent Information
- Application Number
- CN202310552557.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2023-05-17
- Publication Date
- 2025-08-26
- Estimated Expiration
- 2043-05-17
AI Technical Summary
During the freeze-drying process of the existing PCR eight-connected pipe, the pipe cover cannot be fixed, resulting in lax sealing, inaccurate pressure control of the freeze-drying equipment, resulting in tube deformation, cumbersome operation and increased cost, and a long production cycle.
A PCR eight-line tube freeze-drying plate rack is designed, including a base, a tube bracket, a cover bracket and a spring. Through the coordination of the positioning groove, a positioning hole and a spring hole, the tube cover is ensured to be accurately aligned, and the freeze-drying process is accelerated through the height limiting arm and ventilation duct to avoid tube deformation.
The PCR eight-connected pipe lyophilization process is achieved quickly, efficiently and conveniently, ensuring that the pipe cover is sealed, avoiding deformation, shortening the production cycle and reducing costs.
Smart Images
Figure CN116793014B_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to the technical field of biomolecule detection, and in particular to a PCR eight-tube freeze-drying plate rack and a use method thereof. Background Art
[0002] Lyophilization of all components of nucleic acid diagnostic reagents in eight-tube strips offers significant advantages for frontline users. These strips require a vacuum seal and capping process to ensure product performance. This often leads to the following issues: Using the original eight-tube caps for PCR, the caps cannot be fixed in place during the freeze-drying process, preventing proper capping and sealing. Using custom caps can also cause deformation of the eight-tube strips due to the inability to accurately control the pressure applied by the freeze-drying equipment. This necessitates cap replacement during frontline use, leading to cumbersome operation, increased costs, and prolonged freeze-drying time, resulting in a long production cycle. Therefore, we designed a freeze-drying plate rack for eight PCR tubes. Summary of the Invention
[0003] The object of the present invention is to provide a PCR eight-tube freeze-drying plate rack and a method of use to solve the problems raised in the above background technology.
[0004] A PCR eight-tube freeze-drying plate rack comprises a base, a tube holder, a cover holder and a spring. The base is provided with a blind hole, a positioning groove and an armrest for wrapping the PCR eight-tube row. The tube holder has a continuous through hole in the middle, spring holes at the four corners, a ventilation duct on the lower side, and height-limiting arms and positioning holes on both sides. The spring hole has a socket for the cover holder in the upper half, and the cover holder has a continuous rectangular groove. The spring is placed in the spring hole, and the cover holder is inserted into the socket and placed on the tube holder. The tube holder is accurately placed on the base through the positioning groove and the positioning hole.
[0005] Preferably, there are several blind holes, which are evenly distributed on the upper surface of the base. The blind holes and the through holes correspond accurately, so that the bottoms of the PCR eight-tube strip placed on the tube holder fall accurately into the blind holes.
[0006] Preferably, there are two armrests, which are located on both sides of the base.
[0007] Preferably, a plurality of through holes are provided and evenly distributed on the top of the tube holder, so that the position of the eight-row PCR tube can be stabilized.
[0008] Preferably, two height-limiting arms are provided and are located on both sides of the tube holder respectively. The height-limiting arms bear the pressure, and the PCR eight-row tube will not continue to be stressed, thereby avoiding deformation of the PCR eight-row tube.
[0009] A method for using a PCR eight-tube freeze-drying plate rack comprises the following steps:
[0010] S1. Sterilize and dry the base, tube holder, and cover holder;
[0011] S2. Place the pipe support on the base 1 according to the positioning groove so that the through holes on the pipe support correspond to the blind holes on the base;
[0012] S3. Align the eight-tube strip of PCR tubes with the through-holes on the tube holder and place them so that the bottom ends of the eight-tube strip of PCR tubes are inserted into the blind holes on the base.
[0013] S4. Align the cap holder with the socket on the spring hole on the tube holder and lower it so that the tube caps at the bottom of the cap holder correspond one to one with the eight PCR tubes in the tube holder.
[0014] S5. Rapidly freeze-dry the reagents in the eight-tube PCR strip;
[0015] S6. After the freeze-drying is completed, the lower capping bracket drives the tube cap to fall and press tightly on the top of the PCR eight-tube strip to seal it until the bottom of the cap bracket contacts the height-limiting arm.
[0016] Preferably, in step S5, when freeze-drying the eight-tube strip of PCR, ventilation is performed through the ventilation duct to accelerate the freeze-drying of the eight-tube strip of PCR.
[0017] The present invention provides a PCR eight-tube freeze-drying plate rack and a method for use. The PCR eight-tube freeze-drying plate rack and the method for use have the following beneficial effects:
[0018] 1. This PCR eight-tube freeze-drying plate rack and its usage method are equipped with a cover bracket with a continuous rectangular groove. This bracket can stably place the original fluorescent tube caps of the PCR eight-tube strip, ensuring that the original tube caps of the PCR eight-tube strip can be used for freeze-drying. The four corners of the tube bracket included in the PCR eight-tube freeze-drying plate rack are equipped with spring holes, which can be used to place springs to support the tube bracket, allowing it to move up and down within a certain range, ensuring that the PCR tube caps will not affect the water analysis and sublimation in the reagents during freeze-drying, and can be normally capped during sealing. The tube brackets included in the PCR eight-tube freeze-drying plate rack have limited height arms on both sides to ensure that after the PCR eight-tube strip is capped to the appropriate position, the pressure is borne by the limited height arms, and the PCR eight-tube strip will not continue to be stressed, thus avoiding deformation of the PCR eight-tube strip.
[0019] 2. The PCR eight-tube freeze-drying plate rack and its usage method are provided with a ventilation duct on the lower side of the tube holder, which can ensure that the PCR eight-tube strip can be quickly cooled during the freezing and prefreezing stage, and has a weight-reducing effect on the structure. The tube holders included in the PCR eight-tube freeze-drying plate rack have continuous through holes evenly distributed in the middle, which can stabilize the position of the PCR eight-tube strip; the base included in the PCR eight-tube freeze-drying plate rack has positioning grooves and blind holes. The positioning grooves can accurately and stably place the tube holders on the base, and accurately correspond to the blind holes and through holes, so that the bottoms of the PCR eight-tube strip test tubes placed on the tube holders fall accurately into the blind holes, so that the reagent-containing part of the PCR eight-tube strip is in close contact with the metal, which is beneficial to the heating and cooling of the reagents: the above structure makes the freeze-drying of PCR eight-tube strip reagents faster, more effective and more convenient. BRIEF DESCRIPTION OF THE DRAWINGS
[0020] Figure 1 It is a side view of the present invention;
[0021] Figure 2 It is a side view of the base 1 of the present invention;
[0022] Figure 3 This is a side view of the pipe support 2 of the present invention;
[0023] Figure 4 This is a side view of the cover bracket 3 of the present invention;
[0024] Figure 5 This is a perspective view of the tube holder 2, cover holder 3, and spring 4 of the present invention after being assembled and placed with eight PCR tubes in a row;
[0025] Figure 6 This is a side view of the tube holder 2, cover holder 3, and spring 4 of the present invention after being assembled and placed on eight PCR tubes;
[0026] Figure 7 This is a side view of the base 1, tube holder 2, cover holder 3, and spring 4 of the present invention after being assembled and placed with eight PCR tubes in a row.
[0027] In the figure: 1 base, 2 pipe bracket, 3 cover bracket, 4 spring, 5 blind hole, 6 positioning slot, 7 handrail, 8 through hole, 9 spring hole, 10 ventilation duct, 11 height limiting arm, 12 positioning hole, 13 socket, 14 rectangular slot. DETAILED DESCRIPTION
[0028] like Figure 1-7As shown, the present invention provides a technical solution: a PCR eight-tube freeze-drying plate rack, comprising a base 1, a tube holder 2, a cover holder 3, and a spring 4. The base 1 is provided with a blind hole 5, a positioning groove 6, and an armrest 7 for wrapping the PCR eight-tube strip. The tube holder 2 has a continuous through hole 8 in the middle, spring holes 9 at the four corners, and height-limiting arms 11 and positioning holes 12 on both sides. The spring hole 9 has a socket 13 for the cover holder 3 in the upper half. The cover holder 3 has a continuous rectangular groove 14. The spring 4 is placed in the spring hole 9, and the cover holder 3 is inserted into the socket 13 and placed on the tube holder 2. The tube holder 2 is accurately placed on the base 1 through the positioning groove 6 and the positioning hole 12. The cover holder 3 has a continuous rectangular groove 14. Through the rectangular groove 14, the holder can stably place the original fluorescent tube caps of the PCR eight-tube strip, ensuring that the original tube caps of the PCR eight-tube strip can be used for freeze-drying. The PCR eight-tube freeze-drying plate rack includes spring holes 9 at the four corners of the tube holder 2, which accommodate springs 4 to support the tube holder 2, allowing it to move up and down within a certain range. This ensures that the PCR tube caps do not affect the desorption and sublimation of water in the reagents during freeze-drying, and that the caps can be properly sealed during the sealing process. The PCR eight-tube freeze-drying plate rack includes limited height arms 11 on both sides of the tube holder 2. This ensures that after the PCR eight-tube row is capped to the appropriate position, the limited height arms 11 withstand the pressure of the PCR eight-tube row, preventing the PCR eight-tube row from being subjected to further force and thus preventing deformation of the PCR eight-tube row. The PCR eight-tube freeze-drying plate rack includes a tube holder 2 with a ventilation duct 10 on the lower side, which can ensure that the PCR eight-tube strip can be quickly cooled during the pre-freezing stage and has a weight-reducing effect on the structure; the tube holder included in the PCR eight-tube freeze-drying plate rack has continuous through holes evenly distributed in the middle, which can stabilize the position of the PCR eight-tube strip; the base 1 included in the PCR eight-tube freeze-drying plate rack has a positioning groove 6 and a blind hole 5, the positioning groove 6 can enable the tube holder 2 to be accurately and stably placed on the base 1, and the blind hole 5 and the through hole 8 to accurately correspond, so that the bottom of the PCR eight-tube strip placed on the tube holder falls accurately into the blind hole 5, so that the reagent-containing part of the PCR eight-tube strip is in close contact with the metal, which is conducive to the heating and cooling of the reagent: the above structure makes the freeze-drying of the PCR eight-tube strip reagent more rapid, effective and convenient. A method for using the PCR eight-tube strip freeze-drying plate rack includes the following steps:
[0029] S1, disinfecting and drying the base 1, the tube support 2, and the cover support 3;
[0030] S2. Place the pipe support 2 on the base 1 according to the positioning groove 6 so that the through holes 8 on the pipe support 2 correspond one to one with the blind holes 5 on the base 1;
[0031] S3. Align the eight-tube strip of PCR tubes with the through-hole 8 on the tube holder 2 and place them so that the bottom ends of the eight-tube strip of PCR tubes are inserted into the blind holes 5 on the base 1;
[0032] S4. Align the cap holder 3 with the socket 13 on the spring hole 9 on the tube holder 2 and lower it so that the tube caps at the bottom of the cap holder 3 correspond one to one with the eight PCR tubes in the tube holder 2.
[0033] S5. Rapidly freeze-dry the reagents in the 8-tube strip of PCR, and ventilate through the ventilation duct 10 to accelerate the freeze-drying of the 8-tube strip of PCR;
[0034] S6. After the freeze-drying is completed, the lower capping support 3 drives the tube cap to fall and press tightly on the top of the PCR eight-tube strip to seal it until the bottom of the capping support 3 contacts the height-limiting arm 11.
[0035] When the PCR eight-row freeze-drying plate rack is in use, the spring holes 9 provided at the four corners of the tube holder 2 included in the PCR eight-row freeze-drying plate rack can be used to place springs 4 to support the tube holder 2 so that it can move up and down within a certain range, ensuring that the PCR tube caps will not affect the decomposition and sublimation of water in the reagents during freeze drying, and can be normally pressed when sealing. The PCR eight-row tube freeze-drying plate rack includes limited height arms 11 on both sides of the tube holder 2, which ensures that after the PCR eight-row tube is pressed into the appropriate position, the limited height arms 11 bear the pressure, and the PCR eight-row tube will not continue to be subjected to force, thereby avoiding deformation of the PCR eight-row tube. The tube holder 2 included in the PCR eight-row tube freeze-drying plate rack is provided with a ventilation duct 10 on the lower side, which can ensure that the PCR eight-row tube can be quickly cooled during the pre-freezing stage and has a weight-reducing effect on the structure; the tube holder included in the PCR eight-row tube freeze-drying plate rack has continuous through holes evenly distributed in the middle, which can stabilize the position of the PCR eight-row tube; the base 1 included in the PCR eight-row tube freeze-drying plate rack has a positioning groove 6 and a blind hole 5, the positioning groove 6 can make the tube holder 2 accurately and stably placed on the base 1, and make the blind hole 5 and the through hole 8 accurately correspond, so that the bottom of the PCR eight-row tube test tube placed on the tube holder accurately falls into the blind hole 5, so that the reagent-containing part of the PCR eight-row tube is in close contact with the metal, which is conducive to heating and cooling of the reagent.
Claims
1. A PCR eight-tube freeze-drying plate rack, comprising a base (1), a tube support (2), a cover support (3) and a spring (4), characterized in that: The base (1) is provided with a blind hole (5), a positioning groove (6) and an armrest (7) for wrapping the PCR eight-row tube. There are a plurality of blind holes (5), and the blind holes (5) are evenly distributed on the upper surface of the base (1). The tube support (2) has a continuous through hole (8) in the middle, spring holes (9) at the four corners, a ventilation duct (10) at the lower side, and height-limiting arms (11) and positioning holes (12) on both sides. There are a plurality of through holes (8) and they are evenly distributed on the top of the tube support (2). The spring hole (9) has a socket (13) for the cover support (3) in the upper half. The cover support (3) has a continuous rectangular groove (14). The spring (4) is placed in the spring hole (9), and the cover support (3) is inserted into the socket (13) and placed on the tube support (2). The tube support (2) is accurately placed on the base (1) through the positioning groove (6) and the positioning hole (12).
2. The PCR eight-tube freeze-drying plate rack according to claim 1, characterized in that: There are two handrails (7), which are respectively located on both sides of the base (1).
3. The PCR eight-tube freeze-drying plate rack according to claim 1, characterized in that: Two height-limiting arms (11) are provided and are respectively located on both sides of the pipe support (2).
4. A method for using a PCR eight-tube freeze-drying plate rack, characterized by: The following steps are involved: S1. Sterilize and dry the base (1), the tube support (2), and the cover support (3); S2. Place the pipe support (2) on the base (1) according to the positioning groove (6) so that the through holes (8) on the pipe support (2) correspond to the blind holes (5) on the base (1) one by one; S3, align the eight-tube strip of PCR with the through hole (8) on the tube holder (2) and place it so that the bottom end of the eight-tube strip of PCR is inserted into the blind hole (5) on the base (1); S4. Align the cover bracket (3) with the socket (13) on the spring hole (9) on the tube bracket (2) and lower it so that the tube covers at the bottom of the cover bracket (3) correspond one to one with the eight PCR tubes on the tube bracket (2); S5. Rapidly freeze-dry the reagents in the eight-tube PCR strip; S6. After the freeze-drying is completed, the lower capping support (3) drives the tube cap to fall and press tightly on the top of the PCR eight-tube strip to seal it until the bottom of the capping support (3) contacts the height-limiting arm (11).
5. The method for using the PCR eight-tube freeze-drying plate rack according to claim 4, characterized in that: In the step S5, when freeze-drying the eight-tube strip of PCR, ventilation is performed through the ventilation duct (10) to accelerate the freeze-drying of the eight-tube strip of PCR.
Citation Information
Patent Citations
Microporous reaction plate pressing cover box
CN215193769U
PCR (Polymerase Chain Reaction) eight-connecting-pipe freeze-drying plate frame
CN220083432U