A thin layer chromatographic identification method for differentiating cherry and choke cherry

Abstract

The present application relates to a thin layer chromatography identification method, in particular to a thin layer chromatography identification method for distinguishing cherry and needle cherry. The technical problem that the present application solves is that cherry and needle cherry are similar and easily confused for sale and use, and there is no fast and effective method for distinguishing the two. The method of the present application comprises the following steps: 1) preparing reference liquid and sample liquid; 2) placing cherry reference liquid, needle cherry reference liquid and sample liquid on the same GF254 fluorescence plate at the same height to form a sample point; 3) preparing developing agent, the developing agent is prepared by mixing toluene, ethyl acetate, formic acid and methanol in a volume ratio of 6-7:3-5:1-3:2-4; 4) developing; 5) color development and identification. The method of the present application has the advantages of being fast and efficient, having good separation effect, being fast and sensitive for identification, being simple and economical, and being intuitive, and solves the problem of distinguishing cherry and needle cherry.

Description

TECHNICAL FIELD

[0001] The present application relates to a thin layer chromatography identification method, in particular to a thin layer chromatography identification method for identifying and distinguishing cherry and acerola. BACKGROUND

[0002] Cherry is a plant of Rosaceae Prunus, and there are more than 30 species in this genus, mainly distributed in the temperate zone of the northern hemisphere. It has been widely cultivated, and there are 7 species of origin and common cultivation in China, with many cultivated varieties. The most commonly used are Prunus vulgaris Mill. and Prunus avium L.

[0003] Acerola is a plant of Malpighia of Malpighiaceae, and there are about 30 species in this genus, mainly produced in tropical America. One species has been introduced in Guangzhou and Hainan, China. The commonly used acerola is Malpighia glabra L., commonly known as West Indian cherry. Because it looks like a cherry, it is called acerola.

[0004] Acerola is produced in the sand of northeast Brazil. The content of vitamin C is very high. The test report shows that the content of vitamin C in 100 grams of fresh fruit is 1677.6 milligrams (1.68%). It is 31 times that of lemon, 27 times that of strawberry, and 18 times that of kiwi fruit, which is a true "natural vitamin C king". The content of vitamin C in cherry is relatively low.

[0005] Extract is a product produced by a certain extraction process of plant raw materials, which is widely used in traditional Chinese medicine formula granules, health products, solid beverages or functional foods. Acerola extract, as the highest natural product rich in vitamin C, has been favored by consumers. However, because cherry and acerola are very similar in appearance, it is more difficult to distinguish after being made into extract, which leads to many businesses in the market using cheap cherry extract to pretend to be acerola extract, making profits, disturbing the market and cheating consumers. So far, there is no report of a suitable and rapid method for identifying and distinguishing the two. SUMMARY

[0006] The purpose of the present application is to solve the technical problem that cherry and acerola are very similar and easy to be confused for sale and use, and there is no rapid and effective method for identifying and distinguishing the two, and to provide a thin layer chromatography identification method for identifying and distinguishing cherry and acerola.

[0007] The technical solution of the present application is:

[0008] A thin layer chromatography identification method for identifying and distinguishing cherry and acerola, characterized in that it comprises the following steps:

[0009] 1) Preparation of reference liquid and sample liquid

[0010] 1.1) Take cherry raw materials and needle cherry raw materials, respectively, and place them in sealable test containers. Then, add 5-10 times the mass of the cherry raw materials and needle cherry raw materials, respectively, and 70%-100% mass concentration of ethanol, and perform ultrasonic extraction for 30-60 minutes. Then, place the mixture at room temperature, and then filter it using a microporous filter membrane to collect the filtrate as the cherry reference liquid and the needle cherry reference liquid, respectively;

[0011] 1.2) Take the to-be-tested extract to be identified and distinguished, and place it in a sealable test container. Then, add 5-10 times the mass of the to-be-tested extract, and 70%-100% mass concentration of ethanol, and perform ultrasonic extraction for 30-60 minutes. Then, place the mixture at room temperature, and then filter it using a microporous filter membrane to collect the filtrate as the sample liquid;

[0012] 2) Perform thin layer spotting

[0013] Spot the cherry reference liquid, the needle cherry reference liquid, and the sample liquid on the same GF254 fluorescent plate at the same height and different positions to form the spotting origin point;

[0014] 3) Preparation of developing agent

[0015] 3.1) Mix toluene, ethyl acetate, formic acid, and methanol in a volume ratio of 6-7:3-5:1-3:2-4 to obtain the developing agent;

[0016] 3.2) Place the developing agent in a developing cylinder, seal the developing cylinder, and pre-saturate it for 15-20 minutes;

[0017] 4) Development

[0018] Open the developing cylinder, quickly place the spotted GF254 fluorescent plate in the developing cylinder, and make the GF254 fluorescent plate lean against the developing cylinder. When the front of the developing agent rises to a distance of 6-10 cm from the spotting origin point, remove the GF254 fluorescent plate;

[0019] 5) Color development and identification

[0020] 5.1) After drying the GF254 fluorescent plate, first view it under 254 nm ultraviolet light to obtain the first viewing image, and then view it under 365 nm ultraviolet light to obtain the second viewing image;

[0021] 5.2) Identify and distinguish the to-be-tested extract on the first viewing image and the second viewing image, respectively. If the spots of the to-be-tested extract on the two viewing images are the same as the spots of the cherry reference liquid on the viewing image, respectively, then it is cherry. If the spots of the to-be-tested extract on the two viewing images are the same as the spots of the needle cherry reference liquid on the respective viewing image, then it is needle cherry. Otherwise, the to-be-tested extract is of other types.

[0022] Further, in step 5.1), the first viewing figure is obtained by directly observing under 254 nm ultraviolet light; after observing under 254 nm ultraviolet light, the second viewing figure is obtained by spraying with 5%-10% sulfuric acid ethanol developer by mass ratio, heating at 105°C for 3-5 minutes, and then observing under 365 nm ultraviolet light after being placed at room temperature.

[0023] Further, in step 3.1), the volume ratio of toluene, ethyl acetate, formic acid and methanol is 6:3:1:2.

[0024] Further, in step 2), the distance between the sample application origin position and the bottom of the GF254 fluorescent plate is 1.0-1.5 cm.

[0025] Further, in step 4), the GF254 fluorescent plate is taken out when the front of the developing agent reaches 8-10 cm from the sample application origin.

[0026] Further, in step 3.2), the developing cylinder is a double-slot developing cylinder with a size of 10 cm*10 cm, 20 cm*10 cm or 10 cm*20 cm, and the developing agent doses in the double slots are consistent and are pre-saturated for 15 minutes.

[0027] Further, step 1.1) is specifically: taking cherry raw materials and conifer cherry raw materials, respectively placing them in a stoppered triangular flask, then respectively adding 6 times the mass of the cherry raw materials and conifer cherry raw materials, 70% ethanol with a mass concentration, and performing ultrasonic extraction for 60 minutes, then placing at room temperature, and then filtering with a microporous filter membrane to collect the filtrate as a cherry reference liquid and a conifer cherry reference liquid, respectively.

[0028] Step 1.2) is specifically: taking the to-be-identified extract to be distinguished and placed in a stoppered triangular flask, then adding 6 times the mass of the to-be-identified extract, 70% ethanol with a mass concentration, and performing ultrasonic extraction for 60 minutes, then placing at room temperature, and then filtering with a microporous filter membrane to collect the filtrate as a sample liquid.

[0029] Further, in step 5.1), the viewing light source is 365 nm ultraviolet light.

[0030] Step 5.1) is specifically: after blowing dry the reagent on the GF254 fluorescent plate, the first viewing figure is obtained by observing under 254 nm, then spraying with 10% sulfuric acid ethanol solution by mass ratio, heating at 105°C for 3-5 minutes using an electric heating plate, and then observing under 365 nm after being placed at room temperature to obtain the second viewing figure.

[0031] The beneficial effects of the present application are:

[0032] 1. The cherry and needle cherry are distinguished by thin layer chromatography, which has the advantages of rapidity, high efficiency, good separation effect, quick identification, high sensitivity, simplicity, economy and directness, and solves the problem of distinguishing cherry and needle cherry.

[0033] 2. The developing agent is prepared by mixing toluene-ethyl acetate-formic acid-methanol in a volume ratio of 6-7:3-5:1-3:2-4, the separation effect of the obtained spot is good, the characteristic spot is prominent, and there is no spot tailing and spot diffusion phenomenon, the result is intuitive and obvious, and it is easy to judge.

[0034] 3. The GF254 fluorescent plate is used, the difference spots of needle cherry and cherry are obvious, the inspection light source is 254nm ultraviolet light and 365nm ultraviolet light, two kinds of light sources are used to obtain inspection figures respectively, the detection mode is increased, and the result is more referable.

[0035] 4. The 5%-10% sulfuric acid ethanol is used as a color developing agent, the operation is simple, convenient and fast, the color developing spots are more and the spot color is obvious, the contrast is clear, and the result is more referable.

[0036] 5. The present application directly ultrasonically treats the raw material with appropriate ethanol and filters, the filtrate is used as a detection solution, the sample pretreatment is simple, the cost is low, and the operation is easy. DETAILED DESCRIPTION

[0037] Figure 1 The inspection figure obtained by inspecting the sample 1 to the sample 8 in Example 1 of the present application under ultraviolet 254nm is shown in the figure.

[0038] Figure 2 The inspection figure obtained by inspecting the sample 1 to the sample 8 in Example 1 of the present application under ultraviolet 365nm is shown in the figure.

[0039] (In the chromatogram, the abscissa is the sample number, wherein the sample 1 is cherry raw material, which is purchased in the market; the sample 2 is cherry extract 1, which is purchased in the market; the sample 3 is cherry extract 2, which is purchased in the market; the sample 4 is cherry extract 3, which is purchased in the market; the sample 5 is needle cherry raw material, which is purchased in the market; the sample 6 is needle cherry extract 1, which is purchased in the market; the sample 7 is needle cherry extract 2, which is purchased in the market; the sample 8 is needle cherry extract 3, which is purchased in the market) DETAILED DESCRIPTION

[0040] For the problem of distinguishing cherry and needle cherry, the present application provides a thin layer chromatography identification method which is rapid, low-cost, simple and efficient, has strong specificity, the detection result is intuitive and easy to identify. The present application will be described in detail through specific examples and drawings.

[0041] Example 1

[0042] A thin layer chromatography identification method for distinguishing cherry and needle cherry, comprising the following steps:

[0043] 1) Preparation of reference liquid and sample liquid

[0044] 1.1) Take cherry raw materials and needle cherry raw materials, respectively, and place them in a stoppered flask. Then, add 6 times the mass of the cherry raw materials and needle cherry raw materials, respectively, of 70% mass concentration ethanol, and perform ultrasonic extraction for 60 minutes. After cooling to room temperature, filter using a microporous filter membrane, and collect the filtrate as the cherry reference liquid and the needle cherry reference liquid, respectively.

[0045] 1.2) Take the extract to be identified and placed in a stoppered flask. Then, add 6 times the mass of the extract of 70% mass concentration ethanol, and perform ultrasonic extraction for 60 minutes. After cooling to room temperature, filter using a microporous filter membrane, and collect the filtrate as the sample liquid.

[0046] In other embodiments, the amount of ethanol can be 5-10 times the mass of the cherry raw materials, needle cherry raw materials, or extract to be tested, the mass concentration can be 70%-100%, and the ultrasonic extraction time can be 30-60 minutes.

[0047] 2) Perform thin layer spotting

[0048] Spot the cherry reference liquid prepared in step 1.1), the needle cherry reference liquid, and the sample liquid prepared in step 1.2) on the same GF254 fluorescent plate at the same height to form a spotting origin; the distance from the spotting origin to the bottom of the GF254 fluorescent plate is 1.0 cm.

[0049] 3) Preparation of developing agent

[0050] 3.1) Mix toluene, ethyl acetate, formic acid, and methanol in a volume ratio of 6:3:1:2 to obtain 20 mL of developing agent; in other embodiments, the volume ratio of toluene, ethyl acetate, formic acid, and methanol can be prepared in the range of 6-7:3-5:1-3:2-4.

[0051] 3.2) Place the developing agent in a 10x10 cm double-slot developing tank, and keep the developing agent dosage consistent in the double slots. Seal the double-slot developing tank and pre-saturate for 15 minutes. In other embodiments, the size of the double-slot developing tank can also be 20 cm x 10 cm or 10 cm x 20 cm, and the pre-saturation time can be 15-20 minutes.

[0052] 4) Development

[0053] The pre-saturated developing tank is opened, and the sample spotted GF254 fluorescent plate is quickly put into the developing tank, with the GF254 fluorescent plate leaning against the developing tank. When the front of the developing agent reaches 8 cm from the sample spotting origin, the GF254 fluorescent plate is taken out. In other embodiments, the front of the developing agent can reach a range of 6-10 cm from the sample spotting origin.

[0054] 5) Color development and identification

[0055] 5.1) After the developed GF254 fluorescent plate is dried with a hair dryer, the viewing figure is obtained under 254 nm ultraviolet light, then the plate is sprayed with 10% sulfuric acid ethanol developer by mass ratio, and placed on an electric heating plate for heating at 105°C for 3-5 minutes, then cooled and viewed under 365 nm ultraviolet light to obtain the viewing figure. In other embodiments, the mass ratio of the sulfuric acid ethanol developer can be 5%-10%.

[0056] The viewing figures obtained under 254 nm ultraviolet light and 365 nm ultraviolet light are shown in Figure 1 and Figure 2 respectively. Sample No. 1 and sample No. 5 are cherry raw materials and needle cherry raw materials respectively. Under 254 nm ultraviolet light, the needle cherry has a black color spot at Rf≈0.45, while the cherry does not; under 365 nm ultraviolet light, the needle cherry has a bright blue color spot at Rf≈0.30, while the cherry does not, and the needle cherry has a brown blue color spot at Rf≈0.45, while the cherry does not. Under the two light sources, the spot separation effect of cherry and needle cherry is very good, the characteristic spot is prominent, and there is no spot tailing and spot diffusion phenomenon, the result is intuitive and obvious, and easy to distinguish. Nos. 2-4 and Nos. 6-8 are samples of extracts to be tested. As can be seen from the figures, under 254 nm ultraviolet light, the chromatograms of Nos. 2-4 are the same as the Rf value of No. 1, and under 365 nm ultraviolet light, the chromatograms of Nos. 2-4 are also the same as the Rf value of No. 1, indicating that the extracts of Nos. 2-4 are cherry; under 254 nm ultraviolet light, the chromatograms of Nos. 6-8 are the same as the Rf value of No. 5, and under 365 nm ultraviolet light, the chromatograms of Nos. 6-8 are also the same as the Rf value of No. 5, indicating that the extracts of Nos. 6-8 are needle cherry.

[0057] Example 2

[0058] A thin layer chromatography identification method for identifying and distinguishing cherry and needle cherry, comprising the following steps:

[0059] 1) Preparation of reference liquid and sample liquid

[0060] 1.1) Take cherry raw materials and needle-leaf cherry raw materials, respectively, and place them in a stoppered triangular flask. Then, add 5 times the mass of the cherry raw materials and needle-leaf cherry raw materials, respectively, of 100% mass concentration ethanol, and perform ultrasonic extraction for 30 minutes. Then, place the mixture at room temperature, and then filter it using a microporous filter membrane. Collect the filtrate as the cherry reference liquid and the needle-leaf cherry reference liquid, respectively.

[0061] 1.2) Take the to-be-tested extract to be identified and distinguished, and place it in a stoppered triangular flask. Then, add 5 times the mass of the to-be-tested extract of 100% mass concentration ethanol, and perform ultrasonic extraction for 30 minutes. Then, place the mixture at room temperature, and then filter it using a microporous filter membrane. Collect the filtrate as the sample liquid.

[0062] 2) Perform thin layer spotting

[0063] Place the cherry reference liquid, the needle-leaf cherry reference liquid prepared in step 1.1), and the sample liquid prepared in step 1.2) on the same GF254 fluorescent plate at the same height at different positions to form a spotting origin point. The distance from the spotting origin point to the bottom of the GF254 fluorescent plate is 1.3 cm.

[0064] 3) Prepare the developing agent

[0065] 3.1) Mix toluene, ethyl acetate, formic acid, and methanol in a volume ratio of 7:4:2:4 to obtain 20 mL of the developing agent.

[0066] 3.2) Place the developing agent in a 10x10 cm double-tank developing cylinder, and keep the amount of the developing agent in the double tanks consistent. Seal the double-tank developing cylinder, and pre-saturate it for 17 minutes.

[0067] 4) Development

[0068] Open the pre-saturated developing cylinder, and quickly place the spotted GF254 fluorescent plate in the developing cylinder so that the GF254 fluorescent plate is inclined against the developing cylinder. When the front of the developing agent rises to a distance of 6 cm from the spotting origin point, remove the GF254 fluorescent plate.

[0069] 5) Color development and identification

[0070] Dry the developing agent on the thin layer plate using a hair dryer. First, observe it under 254 nm ultraviolet light, then spray it with 5% sulfuric acid ethanol color developing agent, and then place it on an electric heating plate to heat it at 105°C for 3-5 minutes. After cooling, observe it under 365 nm ultraviolet light. The observation figures obtained under 254 nm ultraviolet light and 365 nm ultraviolet light are similar to the observation figures obtained under the corresponding light sources in Example 1. For details, refer to Figure 1 and Figure 2 .

[0071] Example 3

[0072] A thin layer chromatography identification method for distinguishing cherry and needle cherry, comprising the following steps:

[0073] 1) preparing reference liquid and sample liquid

[0074] 1.1) taking cherry raw materials and needle cherry raw materials, respectively placing them in stoppered triangular flasks, then respectively adding 10 times the mass of the cherry raw materials and needle cherry raw materials, 70% mass concentration ethanol, ultrasonic extraction for 60 minutes, then placing at room temperature, then filtering with a microporous filter membrane, respectively collecting the filtrate as the cherry reference liquid and the needle cherry reference liquid.

[0075] 1.2) taking the to-be-identified extract to be distinguished, placing it in a stoppered triangular flask, then adding 10 times the mass of the to-be-identified extract, 70% mass concentration ethanol, ultrasonic extraction for 60 minutes, then placing at room temperature, then filtering with a microporous filter membrane, collecting the filtrate as the sample liquid;

[0076] 2) performing thin layer spotting

[0077] Spotting the cherry reference liquid, the needle cherry reference liquid prepared in step 1.1) and the sample liquid prepared in step 1.2) on the same GF254 fluorescent plate at the same height to form a spotting origin; the distance from the spotting origin to the bottom of the GF254 fluorescent plate is 1.5 cm.

[0078] 3) preparing a developing agent

[0079] 3.1) uniformly mixing toluene, ethyl acetate, formic acid and methanol in a volume ratio of 6:5:3:2 to obtain 20 mL of a developing agent.

[0080] 3.2) placing the developing agent in a 10x10 cm double-tank developing cylinder, keeping the developing agent dosage consistent in the double tanks, sealing the double-tank developing cylinder, and pre-saturating for 20 minutes.

[0081] 4) developing

[0082] Opening the pre-saturated developing cylinder, quickly placing the spotted GF254 fluorescent plate in the developing cylinder, and making the GF254 fluorescent plate obliquely lean in the developing cylinder; when the front of the developing agent rises to a distance of 10 cm from the spotting origin, the GF254 fluorescent plate is taken out.

[0083] 5) color development and identification

[0084] Blowing the developing agent on the thin layer plate dry with a hair dryer, first observing under 254 nm ultraviolet light, then spraying with 10% sulfuric acid ethanol color developing agent, then placing on an electric heating plate and heating at 105°C for 3-5 minutes. After cooling, observing under 365 nm ultraviolet light. The observation figures under 254 nm ultraviolet light and 365 nm ultraviolet light are similar to the observation figures obtained under the corresponding light sources in Example 1, and specific reference can be made toFigure 1 and Figure 2 .

[0085] Case 4

[0086] A thin layer chromatography identification method for distinguishing cherry and needle cherry, comprising the following steps:

[0087] 1) Preparation of reference liquid and sample liquid

[0088] 1.1) Take cherry raw materials and needle cherry raw materials, respectively, and place them in a stoppered flask. Then, add 8 times the mass of cherry raw materials and needle cherry raw materials, respectively, and 80% mass concentration of ethanol, and perform ultrasonic extraction for 50 minutes. After cooling to room temperature, filter using a microporous filter membrane, and collect the filtrate as the cherry reference liquid and the needle cherry reference liquid, respectively.

[0089] 1.2) Take the extract to be identified and placed in a stoppered flask. Then, add 8 times the mass of the extract to be identified, and 80% mass concentration of ethanol, and perform ultrasonic extraction for 50 minutes. After cooling to room temperature, filter using a microporous filter membrane, and collect the filtrate as the sample liquid.

[0090] 2) Perform thin layer spotting

[0091] Spot the cherry reference liquid, needle cherry reference liquid prepared in step 1.1), and sample liquid prepared in step 1.2) on the same GF254 fluorescent plate at the same height to form a spotting origin. The distance from the spotting origin to the bottom of the GF254 fluorescent plate is 1.0 cm.

[0092] 3) Preparation of developing agent

[0093] 3.1) Mix toluene, ethyl acetate, formic acid, and methanol in a volume ratio of 6:3:1:2 to obtain 20 mL of developing agent.

[0094] 3.2) Place the developing agent in a 10x10 cm double-slot developing tank, and keep the developing agent dosage consistent in the double slots. Seal the double-slot developing tank and pre-saturate for 15 minutes.

[0095] 4) Development

[0096] Open the pre-saturated developing tank, and quickly place the spotted GF254 fluorescent plate into the developing tank, with the GF254 fluorescent plate inclined against the developing tank. When the front of the developing agent rises to 9 cm from the spotting origin, remove the GF254 fluorescent plate.

[0097] 5) Color development and identification

[0098] The thin layer plate was dried with a hair dryer. The plate was first examined under UV light at 254 nm, then sprayed with 9% sulfuric acid in ethanol and heated on a hot plate at 105°C for 3-5 minutes. The plate was allowed to cool and examined under UV light at 365 nm. The visualizations obtained under UV light at 254 nm and 365 nm were similar to those obtained under the corresponding light sources in Example 1, and can be referred to in detail therein. Figure 1 and Figure 2 .

Claims

1. A thin layer chromatographic identification method for differentiating cherry and choke cherry, characterized by, The method comprises the following steps: 1) preparing reference liquid and sample liquid 1.1) taking cherry raw materials and needle-leaf cherry raw materials, respectively placing them in sealable test containers, then respectively adding 5-10 times the mass of the cherry raw materials and needle-leaf cherry raw materials, and 70-100% mass concentration ethanol, ultrasonic extraction for 30-60 minutes, then placing at room temperature, then filtering using a microporous filter membrane, and respectively collecting the filtrate as cherry reference liquid and needle-leaf cherry reference liquid; 1.2) taking the to-be-tested extract to be identified and distinguished, placing it in a sealable test container, then adding 5-10 times the mass of the to-be-tested extract, and 70-100% mass concentration ethanol, ultrasonic extraction for 30-60 minutes, then placing at room temperature, then filtering using a microporous filter membrane, and collecting the filtrate as sample liquid; 2) performing thin layer spotting spotting the cherry reference liquid, needle-leaf cherry reference liquid and sample liquid on the same GF254 fluorescent plate at the same height to form a spotting origin; 3) preparing developing agent 3.1) mixing toluene, ethyl acetate, formic acid and methanol in a volume ratio of 6-7:3-5:1-3:2-4 to obtain developing agent; 3.2) placing the developing agent in a developing cylinder, sealing the developing cylinder, and pre-saturating for 15-20 minutes; 4) developing opening the developing cylinder, quickly placing the spotted GF254 fluorescent plate in the developing cylinder, and making the GF254 fluorescent plate obliquely lean against the developing cylinder; when the front of the developing agent rises to 6-10 cm from the spotting origin, taking out the GF254 fluorescent plate; 5) developing color and identification 5.1) after drying the GF254 fluorescent plate, first observing under 254 nm ultraviolet light to obtain a first observation image; then observing under 365 nm ultraviolet light to obtain a second observation image; 5.2) respectively identifying and distinguishing the to-be-tested extract on the first observation image and the second observation image; if the to-be-tested extract spots on the two observation images are respectively the same as the cherry reference liquid spots on the observation images, then the to-be-tested extract is cherry; if the to-be-tested extract spots on the two observation images are respectively the same as the needle-leaf cherry reference liquid spots on the respective observation images, then the to-be-tested extract is needle-leaf cherry; otherwise, the to-be-tested extract is of other types.

2. The thin layer chromatography identification method according to claim 1, wherein in step 5.1), the first observation image is directly observed under 254 nm ultraviolet light; after observation under 254 nm ultraviolet light, before observation under 365 nm ultraviolet light, 5-10% sulfuric acid ethanol color developing agent is sprayed, heated at 105°C for 3-5 minutes, then placed at room temperature, and then observed under 365 nm ultraviolet light to obtain the second observation image.

3. The thin layer chromatography identification method according to claim 2, wherein in step 3.1), the volume ratio of toluene, ethyl acetate, formic acid and methanol is 6:3:1:

2.

4. The thin layer chromatography identification method according to any one of claims 1-3, wherein ​ ​ ​ In step 2), the distance between the sample application origin position and the bottom of the GF254 fluorescent plate is 1.0-1.5 cm.

5. The thin layer chromatography identification method for distinguishing cherry and needle cherry according to claim 4, characterized in that: In step 4), the GF254 fluorescent plate is taken out when the front of the developing agent reaches 8-10 cm from the sample application origin.

6. The thin layer chromatography identification method for distinguishing cherry and needle cherry according to claim 5, characterized in that: In step 3.2), the developing tank is a double-slot developing tank with a size of 10 cm x 10 cm, 20 cm x 10 cm or 10 cm x 20 cm, and the developing agent in the double slots is kept consistent and is pre-saturated for 15 minutes.

7. A thin layer chromatographic identification method for differentiating cherry and prickly cherry according to claim 6, characterized in that, Step 1.1) is specifically: taking cherry raw materials and needle cherry raw materials, respectively placing them in a stoppered triangular flask, then adding 6 times the mass of the cherry raw materials and needle cherry raw materials, respectively, 70% ethanol with a mass concentration, and performing ultrasonic extraction for 60 minutes, leaving to room temperature, then filtering with a microporous filter membrane, and collecting the filtrate as a cherry reference liquid and a needle cherry reference liquid, respectively. Step 1.2) is specifically: taking the to-be-identified extract to be distinguished, placing it in a stoppered triangular flask, then adding 6 times the mass of the to-be-identified extract, 70% ethanol with a mass concentration, and performing ultrasonic extraction for 60 minutes, leaving to room temperature, then filtering with a microporous filter membrane, and collecting the filtrate as a sample liquid.

8. The thin layer chromatography identification method for distinguishing cherry and needle cherry according to claim 7, characterized in that: Step 5.1) is specifically: after blowing dry the reagent on the GF254 fluorescent plate, first observing a first observation figure under 254 nm, then spraying a 10% sulfuric acid ethanol solution, using an electric heating plate to heat at 105°C for 3-5 minutes, leaving to room temperature, and then observing a second observation figure under 365 nm.

Images