Reaction cup cleaning method and cleaning tray for an immunoassay analyzer

Abstract

The application discloses a reaction cup cleaning method of an immune analyzer, which comprises the following steps: in the cleaning process, two different cleaning solutions are adopted, one of the cleaning solutions is used to clean the reaction cup, and then the other cleaning solution is used to remove the residual bubbles in the reaction cup, and finally the reaction cup is sent to the next process for detection. The two different cleaning solutions are used to clean the reaction cup and remove the residual bubbles in the reaction cup, so that the accuracy of the final detection result is ensured.

Description

TECHNICAL FIELD

[0001] The application relates to a reaction cup cleaning method and a cleaning mechanism for realizing the method, in particular to a reaction cup cleaning method and a cleaning disc of an immune analyzer, and belongs to the technical field of medical examination. BACKGROUND

[0002] Since the first automatic chemical analysis instrument was manufactured, more than half a century has passed, and a full-automatic immune analyzer has been mature in technology. The full-automatic immune analyzer has the following characteristics: 1, a multi-degree-of-freedom mechanical arm is adopted to coordinate the actions between modules; 2, the instrument has strong flexibility and can meet various analysis requirements; 3, the test speed is high, and the instrument can continuously run for a long time without human intervention; 4, various technologies are combined in the detection technology, and complete automation is realized in processing, so that the detection result is more accurate and the precision is higher. The full-automatic immune analyzer can realize the steps of taking a reaction cup, adding a sample, adding a reaction liquid, shaking, promoting a reaction, measuring, operation and analysis, cleaning and the like in the experimental test process, and the full-automatic immune analyzer replaces manual operation, not only saves the labor cost, but more importantly, eliminates human errors and ensures the accuracy of data. The full-automatic immune analyzer has the advantages of rapidness, high efficiency, high precision and consistent repeatability, and is widely applied in the fields of processing, production, testing and life assistance, and will inevitably become a trend in the medical detection field.

[0003] In the test process, nanometer magnetic beads and antibodies and antigens need to be combined, and the remaining liquid in the reaction cup needs to be cleaned after the nanometer magnetic beads and the antibodies and antigens are combined, so as to ensure the detection effect. The principle of magnetic separation cleaning is as follows: in the principle of enzyme-promoted chemiluminescence immune technology, after the cloned antibodies in the magnetic bead particle reagent and the to-be-detected substances in the sample, and the enzyme-labeled antibodies or antigens in the reagent are combined through non-specific immune reaction, a magnetic immune complex which can be suspended in a reaction system is formed. In a magnetic field formed by a magnet, the magnetic immune complex and the uncombined magnetic particles are rapidly captured by the magnetic field and attached to the part close to the magnetic field, the liquid outside the magnetic particle complex is removed, so that the magnetic particle complex is separated from the enzyme particles in the liquid. When the external magnetic field is removed and the cleaning liquid is injected, the magnetic particle complex can be uniformly dispersed in the solution, and the cleaning of the free enzyme is completed for many times. As the reaction carrier of the antigen and antibody, the magnetic beads can make the immune reaction occur rapidly under uniform conditions, and promote the rapid separation of the immune complex from the solution.

[0004] In the immune analyzer, the cleaning disc is responsible for cleaning the reaction cup. In the prior art, the problem is that a large amount of air bubbles will be left in the reaction cup after the cleaning liquid is added and the cleaning is completed, so as to affect the accuracy of the final detection result.

[0005] After searching, the following patent documents are found:

[0006] I. Chinese patent application publication CN104297039A published on January 21, 2015 discloses a novel magnetic separation mechanism, which comprises a coded turntable mechanism, a magnet opening and closing mechanism and a cleaning mechanism; the magnet opening and closing mechanism is installed on the coded turntable mechanism, and the cleaning mechanism is distributed around the coded turntable mechanism; the magnet opening and closing mechanism controls the nanometer magnetic beads in the liquid in the reaction cup placed on the coded turntable mechanism, and the cleaning mechanism sequentially cleans the liquid in the reaction cup to complete the separation of the nanometer magnetic beads; the coded turntable mechanism comprises a turntable, a fixed disc, support nails, a bearing seat provided with bearings, a turntable rotating shaft, a first synchronous belt driven wheel, a first synchronous belt driving wheel, a first synchronous belt, a first stepping motor, a mounting plate, a fixed support frame, a first zero position sensor, a first sensing sheet and a first zero position sensor fixing seat; the mounting plate is fixed horizontally on the top of the fixed support frame, and the fixed disc is fixed on the mounting plate by at least two support nails; the bearing seat provided with bearings is installed on the mounting plate; the turntable is fixed on the upper end of the turntable rotating shaft; the turntable rotating shaft is connected with the bearings in cooperation, and the lower end of the turntable rotating shaft penetrates through the mounting plate and is connected with the first synchronous belt driven wheel; the first synchronous belt driving wheel is connected with the first synchronous belt driven wheel through the first synchronous belt; the first stepping motor is installed on the side of the fixed support frame; the first stepping motor finally drives the turntable to rotate through the first synchronous belt driving wheel, the first synchronous belt, the first synchronous belt driven wheel and the turntable rotating shaft; the first zero position sensor fixing seat is arranged on the bottom of the mounting plate, the first zero position sensor is installed on the first zero position sensor fixing seat, and the first zero position sensing sheet is installed on the first synchronous belt driven wheel; the turntable is uniformly provided with reaction cup holes for placing reaction cups, and a guide groove for placing a magnet sliding block is formed below the reaction cup holes; the magnet opening and closing mechanism comprises the magnet sliding block, a permanent magnet, a roller, a first pin shaft and a cam groove formed on the fixed disc; the permanent magnet is embedded in the magnet sliding block, one end of the first pin shaft is connected with the roller, and the other end is tightly fitted and installed in the magnet sliding block; the magnet sliding block is embedded in the guide groove of the turntable with the permanent magnet facing outward, and the roller on the magnet sliding block is installed in the cam groove of the fixed disc; with the rotation of the turntable, the roller of the magnet sliding block rolls along the cam groove, the magnet sliding block moves radially on the guide groove, and the reaction cup is adsorbed; the cleaning mechanism comprises a guide shaft, a guide wheel, a cleaning sliding block, a cleaning needle, a second driven belt wheel, a second synchronous belt, a second synchronous belt wheel, a Hall sensor, a second stepping motor, a second stepping motor fixing plate and a cleaning fixing bottom plate; the second stepping motor fixing plate is vertically installed on the cleaning fixing bottom plate, the second stepping motor is fixed on the lower end of the second stepping motor fixing plate, the second stepping motor drives the second synchronous belt wheel, and the second driven belt wheel installed on the upper end of the second stepping motor fixing plate is connected through the second synchronous belt transmission;The cleaning slide block is provided with a cleaning needle, and the cleaning slide block is fixedly connected with the second synchronous belt and moves up and down with the second synchronous belt.

[0007] II. Chinese invention patent with application publication number CN110873660A and application publication date of March 10, 2020 discloses a magnetic separation device, characterized in that it comprises: a magnetic separation unit, which comprises: a magnetic separation disc, at least one cup placing position is arranged on the magnetic separation disc to accommodate a reaction container, wherein the reaction container contains magnetic beads and a solution for performing an immune analysis test; and at least one annular magnet is placed in the at least one cup placing position, wherein a corresponding annular magnet is placed in each cup placing position, so that when the reaction container is placed in the cup placing position, the magnetic force of the annular magnet in the cup placing position is used to adsorb the magnetic beads on the inner wall of the reaction container, thereby avoiding the magnetic beads from being discharged when the waste liquid in the reaction container is discharged.

[0008] From the above patent documents, it can be known that in the prior art, when the magnetic beads in the reaction cup are cleaned, no matter how many times the cleaning is performed, it is operated through the basic process of "liquid injection-liquid suction". In actual work, the problem is that after the reaction cup is subjected to the last liquid suction, some air bubbles are left in the reaction cup before it is sent to the next program for detection, thereby affecting the detection result of the next step.

[0009] In summary, how to design a reaction cup cleaning method and a cleaning disc of an immune analyzer so as to minimize the air bubbles left in the reaction cup after the last liquid suction and ensure the accuracy of the detection result of the next step is a technical problem to be solved. SUMMARY

[0010] The technical problem to be solved by the present application is to solve the defects in the prior art, and a reaction cup cleaning method and a cleaning disc of an immune analyzer are disclosed, which can ensure that no air bubbles are left in the reaction cup after the last liquid washing, so as to ensure the accuracy of the final detection result.

[0011] To solve the above technical problems, the technical solution adopted by the present application is: a reaction cup cleaning method of an immune analyzer, which uses two different cleaning liquids in the cleaning process, uses one of the cleaning liquids to clean the reaction cup first, and then uses the other cleaning liquid to remove the air bubbles left in the reaction cup, and then sends the reaction cup to the next process for detection.

[0012] Preferably, the liquid injection step and the liquid suction step for the reaction cup are arranged as one basic cleaning unit, and the number of the basic cleaning units in the reaction cup cleaning is N1, then N1≥2; in the N1 basic cleaning units, one basic cleaning unit before detection, i.e. the last basic cleaning unit, is set as a basic cleaning unit two, and the other basic cleaning units are set as basic cleaning units one, two cleaning liquids, cleaning liquid A and cleaning liquid B, are used in the whole cleaning process, the reaction cup is injected with cleaning liquid A in the liquid injection step of the basic cleaning unit one, and the reaction cup is cleaned by using the cleaning liquid A, and the reaction cup is injected with cleaning liquid B in the liquid injection step of the basic cleaning unit two, and the cleaning liquid B is used to remove the air bubbles remaining in the reaction cup.

[0013] Preferably, the reaction cup is placed on a rotating disc, and the basic cleaning units are arranged at the upper position of the rotating disc.

[0014] The distribution positions of the basic cleaning unit one and the basic cleaning unit two do not coincide, and the basic cleaning unit one and the basic cleaning unit two are sequentially distributed along the circumference of the rotating disc, one liquid injection station and one liquid suction station are arranged in each cleaning unit, one liquid suction needle is arranged in each liquid suction station, one liquid injection needle one is arranged in the liquid injection station of the basic cleaning unit one, and two liquid injection needles, i.e. liquid injection needle one and liquid injection needle two, are arranged in the liquid injection station of the basic cleaning unit two.

[0015] During cleaning, the rotating disc drives the rotation of the reaction cup, so that the reaction cup passes through the basic cleaning unit, thereby completing the cleaning work; the number of rotations of the rotating disc driving the rotation of the reaction cup is N2, then N2≥2.

[0016] Preferably, when N2=2, the cleaning step includes S1 first circle cleaning step and S2 second circle cleaning step, and the S1 first circle cleaning step includes:

[0017] I) controlling the rotating disc to drive the rotation of the reaction cup to the liquid injection station in the first basic cleaning unit one, and stopping the rotation;

[0018] II) controlling the liquid injection needle in the liquid injection station in the first basic cleaning unit one to descend into the reaction cup, injecting the reaction cup with cleaning liquid A, and cleaning the reaction cup by using the injected cleaning liquid A; after the injection is completed, controlling the liquid injection needle one in the liquid injection station in the first basic cleaning unit one to rise back to the original position;

[0019] III) controlling the rotating disc to drive the rotation of the reaction cup to the liquid suction station in the first basic cleaning unit one, and stopping the rotation;

[0020] IV) controlling the liquid suction needle in the liquid suction station in the first basic cleaning unit to descend into the reaction cup and to suck and discharge the cleaning liquid in the reaction cup; after the discharge is completed, controlling the liquid suction needle in the liquid suction station in the first basic cleaning unit to ascend to the original position;

[0021] Then, the next basic cleaning unit is operated according to the first step to the fourth step until all the basic cleaning units in the first circle are operated.

[0022] Then, the following steps are continued to be operated:

[0023] V) controlling the rotating disc to drive the reaction cup to rotate to the liquid injection station in the second basic cleaning unit and to stop rotating;

[0024] VI) controlling the liquid injection needle in the liquid injection station in the second basic cleaning unit to descend into the reaction cup, injecting the cleaning liquid A into the reaction cup, and cleaning the reaction cup by using the injected cleaning liquid A; after the injection is completed, controlling the liquid injection needle in the liquid injection station in the second basic cleaning unit to ascend to the original position;

[0025] VII) controlling the rotating disc to drive the reaction cup to rotate to the liquid suction station in the second basic cleaning unit and to stop rotating;

[0026] VIII) controlling the liquid suction needle in the liquid suction station in the second basic cleaning unit to descend into the reaction cup and to suck and discharge the cleaning liquid in the reaction cup; after the discharge is completed, controlling the liquid suction needle in the liquid suction station in the second basic cleaning unit to ascend to the original position;

[0027] The S1 first circle cleaning step is completed through the above steps.

[0028] The S2 second circle cleaning step is different from the S1 first circle cleaning step in the sixth step, and other steps are the same as the S1 first circle cleaning step, that is:

[0029] VI) controlling the liquid injection needle in the liquid injection station in the second basic cleaning unit to descend into the reaction cup, injecting the cleaning liquid B into the reaction cup, and cleaning the reaction cup by using the injected cleaning liquid B; after the injection is completed, controlling the liquid injection needle in the liquid injection station in the second basic cleaning unit to ascend to the original position.

[0030] Preferably, when N2>2 circles, the cleaning step includes T1 other circle cleaning step and T2 last circle cleaning step, the T1 other circle cleaning step is the same as the S1 first circle cleaning step, and the T2 last circle cleaning step is the same as the S2 second circle cleaning step.

[0031] Preferably, the reaction cup is placed on a rotating disc, and the basic cleaning unit is arranged at a position above the rotating disc;

[0032] The distribution of the basic cleaning unit one and the basic cleaning unit two coincide, and a plurality of basic cleaning units are at the same position above the rotating disc, a liquid injection station and a liquid suction station are arranged in the basic cleaning unit, a liquid suction needle is arranged in the liquid suction station, and two liquid injection needles, i.e., a liquid injection needle one and a liquid injection needle two, are arranged in the liquid injection station;

[0033] During cleaning, the rotating disc drives the reaction cup to rotate, so that the reaction cup sequentially passes through the basic cleaning unit, thereby completing the cleaning work; the number of rotations of the rotating disc driving the reaction cup is N2, and N2≥2.

[0034] Preferably, the cleaning step includes a D1 other circle cleaning step and a D2 last circle cleaning step, and the D1 other circle cleaning step includes:

[0035] I) controlling the rotating disc to drive the reaction cup to rotate to the liquid injection station in the basic cleaning unit and stop rotating;

[0036] II) controlling the liquid injection needle in the liquid injection station in the basic cleaning unit to descend into the reaction cup, injecting cleaning liquid A into the reaction cup, and cleaning the reaction cup by using the injected cleaning liquid A; after the injection is completed, controlling the liquid injection needle one in the liquid injection station in the basic cleaning unit to rise back to the original position;

[0037] III) controlling the rotating disc to drive the reaction cup to rotate to the liquid suction station in the basic cleaning unit and stop rotating;

[0038] IV) controlling the liquid suction needle in the liquid suction station in the basic cleaning unit to descend into the reaction cup, and sucking and discharging the cleaning liquid in the reaction cup; after the discharging is completed, controlling the liquid suction needle in the liquid suction station in the basic cleaning unit to rise back to the original position;

[0039] The D1 other circle cleaning step is completed through the above steps;

[0040] The D2 last circle cleaning step is different from the D1 other circle cleaning step in the second step, i.e.:

[0041] II) controlling the liquid injection needle two in the liquid injection station in the basic cleaning unit to descend into the reaction cup, injecting cleaning liquid B into the reaction cup, and cleaning the reaction cup by using the injected cleaning liquid B; after the injection is completed, controlling the liquid injection needle two in the liquid injection station in the basic cleaning unit to rise back to the original position.

[0042] The present invention also discloses a cleaning disc, which includes a cleaning disc cylinder, a turntable mechanism disposed inside the cleaning disc cylinder, and a needle lifting mechanism disposed above the cleaning disc cylinder; a reaction cup is placed on the turntable of the turntable mechanism, and the turntable can drive the reaction cup to rotate. Along the circumference of the turntable, two or more basic cleaning units are sequentially arranged on the needle lifting mechanism. The basic cleaning unit includes a basic cleaning unit one and a basic cleaning unit two. Each basic cleaning unit one is provided with an injection needle one and an aspiration needle. The basic cleaning unit two is provided with an integrated injection double needle and an aspiration needle. The integrated injection double needle includes an injection needle one and an injection needle two.

[0043] Driven by the downward movement of the needle body lifting mechanism, both the injection needle and the aspiration needle can descend and be inserted into the reaction cup to perform injection and aspiration operations. Driven by the upward movement of the needle body lifting mechanism, both the injection needle and the aspiration needle can be pulled out of the reaction cup and returned to their original positions.

[0044] Preferably, the integrated injection dual needles are connected to the lifting plate of the needle lifting mechanism via a limiting block. The limiting block includes a seat on the lifting plate and a screw cap threadedly connected to the seat. The seat includes a base plate and a column on the base plate. The column and the base plate are connected through a mounting through hole. A groove is provided on the inner circumferential surface of the column through the mounting through hole. The groove is recessed radially along the column and one side of the groove is open while the other three sides are closed.

[0045] A screw cap through hole is opened at the bottom of the screw cap, and a guide block is also provided on the integrated injection double needle. The guide block is cylindrical and its diameter matches the diameter of the mounting through hole. A radially protruding protrusion is provided on one side of the guide block. During installation, injection needle one and injection needle two are passed through the screw cap through hole of the screw cap, the guide block and the mounting through hole of the seat in sequence, so that the protrusion of the guide block is engaged in the groove. The groove and the protrusion cooperate to limit injection needle one and injection needle two in the circumferential direction. Then the screw cap is tightened on the cylinder, and the screw cap presses the protrusion into the groove, thereby limiting injection needle one and injection needle two in the axial direction.

[0046] Preferably, the needle lifting mechanism further includes a lifting motor, a guide sleeve is provided on the lifting plate, and a guide rod is provided on the top surface of the cleaning disc cylinder. The lifting plate is slidably connected to the guide rod by the guide rod and the guide sleeve. The lifting motor is provided on the cleaning disc cylinder, and its output shaft is connected to a lead screw drive. A nut sleeve is provided on the lifting plate. The lead screw and the nut sleeve cooperate to form a lead screw and nut mechanism, so that the lifting motor can control the up and down movement of the lifting plate. Both the injection needle and the suction needle are provided on the lifting plate.

[0047] The beneficial effects of this invention are as follows: This invention utilizes two different cleaning solutions in combination to both clean the magnetic beads in the reaction vessel and remove residual air bubbles, thereby ensuring the accuracy of the final test results. By designing specific steps for injecting the two cleaning solutions sequentially through two injection needles at different cleaning stages, the removal of residual air bubbles is achieved. By designing a cleaning disc, the above-mentioned cleaning method for the reaction vessel is implemented, removing residual air bubbles and thus ensuring the accuracy of the final test results. Attached Figure Description

[0048] Figure 1 This is a schematic diagram illustrating the steps and principles of the reaction cup cleaning method in an embodiment of the present invention;

[0049] Figure 2 This is a schematic diagram illustrating the structural principle of the reaction cup cleaning method in Embodiment 1 of the present invention;

[0050] Figure 3 This is a three-dimensional structural diagram of the cleaning disc in Embodiment 1 of the present invention;

[0051] Figure 4 for Figure 3 A schematic diagram of the axial cross-section structure;

[0052] Figure 5 for Figure 4 A top-view structural diagram;

[0053] Figure 6 for Figure 4 A partial cross-sectional view of the structure located at the turntable;

[0054] Figure 7 This is a three-dimensional structural diagram of the turntable mechanism in Embodiment 1 of the present invention;

[0055] Figure 8 This is a schematic diagram of the front view of the turntable mechanism in Embodiment 1 of the present invention;

[0056] Figure 9 This is a schematic diagram of the magnetic field distribution location in Embodiment 1 of the present invention;

[0057] Figure 10 for Figure 4 A partial cross-sectional view of the structure located at the needle lifting mechanism;

[0058] Figure 11 This is a three-dimensional structural diagram of the needle lifting mechanism in Embodiment 1 of the present invention;

[0059] Figure 12 for Figure 11 A schematic diagram of the partial three-dimensional structure of the integrated injection dual needles;

[0060] Figure 13 This is a three-dimensional structural diagram of the seat of the limiting block in the cleaning tray according to Embodiment 1 of the present invention;

[0061] Figure 14 This is a three-dimensional structural diagram of the integrated dual injection needles after passing through the screw cap and guide block in Embodiment 1 of the present invention;

[0062] Figure 15 This is a three-dimensional structural diagram of the integrated dual injection needle in Embodiment 1 of the present invention installed on the lifting plate;

[0063] Figure 16 This is a schematic diagram illustrating the structural principle of the reaction cup cleaning method in Embodiment 2 of the present invention;

[0064] In the diagram: 1. Basic cleaning unit one, 2. Basic cleaning unit two, 3. Turntable, 311. Cup hole, 4. Liquid injection station, 5. Liquid suction station, 6. Liquid suction needle, 7. Liquid injection needle one, 8. Liquid injection needle two, 9. 911. Cleaning disc body; 912. Upper cylinder space; 913. Lower cylinder space; 914. Through hole; 10. Turntable mechanism; 101. Turntable motor and reducer; 102. Rotating shaft; 11. Needle lifting mechanism; 111. Lifting plate; 112. Lifting motor; 12. Reaction cup; 13. Sensor 1; 14. Sensing ring; 141. Sensing groove; 15. Sensor 2; 16. Magnet fixing ring; 17. Magnetic field; 18. Guide sleeve; 19. Guide rod; 20. Lead screw; 21. Nut sleeve; 22. Upper position sensor; 23. Lower position sensor; 24. Support rod; 25. Sensing plate; 26. Seat; 261. Base plate; 262. Column; 263. Mounting through hole; 27. Screw cap; 28. Groove; 29. ​​Guide block; 291. Protrusion. Detailed Implementation

[0065] The technical solution of the present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments.

[0066] like Figure 1As shown, a method for cleaning reaction cups in an immunoassay analyzer comprises two basic cleaning units: one for injecting liquid into the reaction cup and the other for aspirating liquid from the reaction cup. The number of these basic cleaning units is designated as N1, where N1 ≥ 2. Within these N1 basic cleaning units, the last basic cleaning unit (before detection) is designated as Basic Cleaning Unit 2, and the others are designated as Basic Cleaning Unit 1. Two cleaning solutions, Cleaning Solution A and Cleaning Solution B, are used throughout the cleaning process. In the injection step of Basic Cleaning Unit 1, Cleaning Solution A is injected into the reaction cup. In the injection step of Basic Cleaning Unit 2, Cleaning Solution B is injected into the reaction cup. Cleaning Solution A has a stronger cleaning ability, but it leaves more residual air bubbles after cleaning. Cleaning Solution B removes residual air bubbles. Upon entering Basic Cleaning Unit 1, the injected Cleaning Solution A cleans the magnetic beads in the reaction cup. Upon entering Basic Cleaning Unit 2, the injected Cleaning Solution B removes residual air bubbles from the reaction cup.

[0067] By using two different cleaning solutions in combination, it is possible to both clean the magnetic beads in the reaction vessel and remove residual air bubbles.

[0068] There are many specific solutions for removing residual air bubbles using two cleaning solutions, but in this application, it is achieved by using an integrated dual injection needle in the injection station 4 of the basic cleaning unit 2, as detailed below: Example 1

[0069] The locations of basic cleaning unit 1 and basic cleaning unit 2 do not overlap:

[0070] like Figure 2 As shown, basic cleaning unit 1 and basic cleaning unit 2 are sequentially distributed along the circumference of the turntable 3 at the upper position of the turntable 3. In this embodiment, N1=3, that is, three basic cleaning units are provided, of which there are two basic cleaning units 1 and one basic cleaning unit 2. Each cleaning unit is provided with one injection station 4 and one suction station 5, for a total of three injection stations 4 and three suction stations 5. Each suction station 5 is provided with one suction needle 6. Each injection station 4 of basic cleaning unit 1 is provided with one injection needle 7, and each injection station 4 of basic cleaning unit 2 is provided with two injection needles integrated together, namely injection needle 7 and injection needle 8.

[0071] During cleaning, the turntable 3 drives the reaction cup (not shown in the figure) to rotate, so that the reaction cup passes through basic cleaning unit 1 and basic cleaning unit 2 in sequence, thereby completing the cleaning work. Let the number of rotations of the reaction cup driven by the turntable 3 be N2, then N2≥2.

[0072] When N2 = 2 cycles, the cleaning steps include a first cycle cleaning step S1 and a second cycle cleaning step S2. The first cycle cleaning step S1 includes:

[0073] 1) Control turntable 3 to drive the reaction cup (not shown in the figure) to rotate to the liquid injection station 4 in the first basic cleaning unit 1, and then stop rotating;

[0074] (ii) Control the injection needle 7 in the injection station 4 of the first basic cleaning unit 1 to descend and extend into the reaction cup, inject cleaning solution A into the reaction cup, and use the injected cleaning solution A to clean the magnetic beads in the reaction cup; after the injection is completed, control the injection needle 7 in the injection station 4 of the first basic cleaning unit 1 to rise back to its original position.

[0075] 3) Control the turntable 3 to drive the reaction cup (not shown in the figure) to rotate to the liquid suction station 5 in the first basic cleaning unit 1, and then stop rotating;

[0076] (iv) Control the suction needle 6 in the suction station 5 of the first basic cleaning unit 1 to descend and extend into the reaction cup to suction and discharge the cleaning liquid in the reaction cup; after discharge, control the suction needle 6 in the suction station 5 of the first basic cleaning unit 1 to rise back to its original position.

[0077] 5) Control the turntable 3 to drive the reaction cup (not shown in the figure) to rotate to the liquid injection station 4 in the second basic cleaning unit 1, and then stop rotating;

[0078] (vi) Control the injection needle 7 in the injection station 4 of the second basic cleaning unit 1 to descend and extend into the reaction cup, inject cleaning solution A into the reaction cup, and use the injected cleaning solution A to clean the magnetic beads in the reaction cup; after the injection is completed, control the injection needle 7 in the injection station 4 of the second basic cleaning unit 1 to rise back to its original position.

[0079] 7) Control the turntable 3 to drive the reaction cup (not shown in the figure) to rotate to the liquid suction station 5 in the second basic cleaning unit 1, and then stop rotating;

[0080] 8) Control the liquid suction needle 6 in the liquid suction station 5 in the second basic cleaning unit 1 to descend and extend into the reaction cup to suction and discharge the cleaning liquid in the reaction cup; after the discharge is completed, control the liquid suction needle 6 in the liquid suction station 5 in the second basic cleaning unit 1 to rise back to its original position.

[0081] 9) Control the turntable 3 to drive the reaction cup (not shown in the figure) to rotate to the liquid injection station 4 in the basic cleaning unit 2, and then stop rotating;

[0082] 10) Control the injection needle 7 in the injection station 4 of the basic cleaning unit 2 to descend and extend into the reaction cup, inject cleaning solution A into the reaction cup, and use the injected cleaning solution A to clean the magnetic beads in the reaction cup; after the injection is completed, control the injection needle 7 in the injection station 4 of the basic cleaning unit 2 to rise back to its original position.

[0083] XI) Control the turntable 3 to drive the reaction cup (not shown in the figure) to rotate to the liquid suction station 5 in the basic cleaning unit 2, and then stop rotating;

[0084] 12) Control the liquid suction needle 6 in the liquid suction station 5 in the second basic cleaning unit 2 to descend and extend into the reaction cup to suck up and discharge the cleaning liquid in the reaction cup; after the discharge is completed, control the liquid suction needle 6 in the liquid suction station 5 in the first basic cleaning unit 1 to rise back to its original position.

[0085] The first cleaning step of S1 is completed by following the steps described above.

[0086] The difference between the second cleaning cycle S2 and the first cleaning cycle S1 lies in step ten; all other steps are the same as the first cleaning cycle S1.

[0087] 10) Control the injection needle 28 in the injection station 4 of the basic cleaning unit 2 to descend and extend into the reaction cup, inject cleaning solution B into the reaction cup, and use the injected cleaning solution B to clean the magnetic beads in the reaction cup; after the injection is completed, control the injection needle 28 in the injection station 4 of the basic cleaning unit 2 to rise back to its original position.

[0088] When N2 > 2 cycles, the cleaning steps include the T1 other cycle cleaning step and the T2 last cycle cleaning step. The T1 other cycle cleaning step is the same as the S1 first cycle cleaning step, and the T2 last cycle cleaning step is the same as the S2 second cycle cleaning step.

[0089] This embodiment also discloses a cleaning tray for implementing the above-described cleaning method, such as... Figures 3 to 5As shown, the cleaning tray includes a cleaning tray cylinder 9, a turntable mechanism 10 disposed inside the cleaning tray cylinder 9, and a needle lifting mechanism 11 disposed above the cleaning tray cylinder 9. A reaction cup is placed on a turntable 3 of the turntable mechanism 10, which drives the reaction cup to rotate. Along the circumference of the turntable 3, two basic cleaning units and one basic cleaning unit are sequentially arranged on the needle lifting mechanism 11. Each basic cleaning unit is equipped with an injection needle 7 and an aspiration needle 6. The basic cleaning unit is equipped with an integrated dual injection needle S and an aspiration needle 6. The integrated dual injection needle S includes an injection needle 7 and an injection needle 8. Along the circumference of the turntable 3, the needles are distributed on the needle lifting mechanism 11 in the following order: injection needle 7, aspiration needle 6, injection needle 7, aspiration needle 6, integrated dual injection needle S, and aspiration needle 6. Driven by the downward movement of the needle lifting mechanism 11, both the injection needle and the aspiration needle can descend and be inserted into the reaction cup for injection and aspiration operations. Conversely, driven by the upward movement of the needle lifting mechanism 11, both the injection needle and the aspiration needle can be withdrawn from the reaction cup and returned to their original positions. Furthermore, the bottom opening of the injection needle is angled, allowing the cleaning fluid sprayed from the bottom opening to impact the reaction cup. This is because a magnetic field is applied to the reaction cup at the aspiration station, causing magnetic beads to be attracted to the inner wall of the reaction cup. Even after the magnetic field disappears, some magnetic beads remain attached to the inner wall. The angled bottom opening of the injection needle further ensures that the cleaning fluid can disperse the magnetic beads adsorbed on the inner side of the reaction cup, thus further guaranteeing the cleaning effect. A notch is provided on the bottom side wall of the aspiration needle near its bottom opening. This is to prevent the aspiration needle from blocking the bottom opening when inserted into the bottom of the reaction cup, ensuring the normal operation of the aspiration process.

[0090] like Figure 4 As shown, a cleaning plate partition 911 is provided inside the cleaning plate cylinder 9, dividing the interior of the cleaning plate cylinder 9 into an upper cylinder space 912 located at the top and a lower cylinder space 913 located at the bottom. The turntable 3 of the turntable mechanism 10 is located in the upper cylinder space 912. Insulation material and a heating device (not shown in the figure) are provided inside the upper cylinder space 912, and the heating device is an electric heating film. The insulation material and heating device heat and maintain the interior of the upper cylinder space 912 at a certain temperature. This is because a certain operating temperature needs to be maintained after the reaction cup is placed on the turntable. In this embodiment, by placing the turntable in the insulation space of the upper cylinder space, it is possible to ensure that the reaction cup on the turntable is always in a certain operating temperature environment.

[0091] like Figures 5 to 7As shown, the turntable mechanism 10 also includes a turntable motor and a reducer 101. The turntable motor and reducer 101 are mounted on the cleaning tray partition 911, and the rotating shaft 102 of the turntable motor and reducer 101 extends into the upper cylinder space 912 and connects to the turntable 3. In the prior art, turntables are driven by belt drives, which makes the transmission structure relatively complex and occupies a large space. In this embodiment, the turntable motor, which is integrated with the reducer, is directly connected to the turntable, eliminating the belt drive structure, thereby simplifying the structure of the rotating mechanism and reducing the space occupied.

[0092] Multiple cup holes 311 for placing reaction cups are arranged circumferentially on the turntable 3. A through hole 914 is opened on the top of the cleaning disc cylinder 9. The through hole 914 is used to place and remove reaction cups 12. The through hole 914 is at the zero point position. When a cup hole 311 on the turntable 3 rotates to coincide with the central axis of the through hole 914, a reaction cup 12 is placed into the cup hole 311 through the through hole 914 or removed from the cup hole 311 through the through hole 914. A sensor 13 is also provided on the side of the cleaning disc cylinder 9. The sensor 13 is used to detect the number of reaction cups on the turntable and the presence or absence of reaction cups on the turntable. Figure 8 As shown, a sensing ring 14 is provided at the bottom of the turntable 3, and multiple sensing grooves 141 are opened on the sensing ring 14. A second sensor 15 is also provided on the cleaning tray partition 911 and located at the sensing ring 14. When the turntable rotates, the second sensor 15 senses the sensing grooves 141 on the sensing ring 14 to control the rotation angle and position of the turntable.

[0093] like Figure 6 and Figure 9As shown, a magnetic fixing ring 16 is also provided on the inner circumferential surface of the cleaning disc cylinder 9, located at the turntable 3. The magnet is positioned on the magnetic fixing ring 16, creating a magnetic field 17 within the cleaning disc cylinder 9 and between the injection station 4 and the suction station 5 in each basic cleaning unit. The interior of the cleaning disc cylinder 9 at the injection station 4 is located outside the magnetic field 17, and the interior of the cleaning disc cylinder 9 at the suction station 5 is located inside the magnetic field 17. When cleaning fluid is injected into the reaction cup at the injection station 4, the reaction cup enters the magnetic field 17 as the turntable rotates. The magnetic field 17 attracts the magnetic beads in the reaction cup until it rotates to the suction station 5. During suction at the suction station 5, the magnetic field 17 holds the magnetic beads in the reaction cup, preventing them from being sucked away by the suction needle. After suction is complete, the reaction cup moves out of the magnetic field 17 and rotates to the injection station 4, where there is no magnetic field. The injection needle then injects fluid into the reaction cup, impacting the magnetic beads and the reaction cup, thus achieving the cleaning purpose. In this embodiment, three magnetic fields 17 are formed. The magnets can be permanent magnets or electromagnets.

[0094] like Figure 10 and Figure 11 As shown, the needle lifting mechanism 11 includes a lifting plate 111 and a lifting motor 112. A guide sleeve 18 is provided on the lifting plate 111, and a guide rod 19 is provided on the top surface of the cleaning disc cylinder 9. The lifting plate 111 is slidably connected to the guide rod 19 by cooperating with the guide sleeve 18. The lifting motor 112 is provided on the cleaning disc cylinder 9, and its output shaft is drivenly connected to the lead screw 20. A nut sleeve 21 is provided on the lifting plate 111. The lead screw 20 and the nut sleeve 21 cooperate to form a lead screw nut mechanism, so that the lifting plate 111 can be moved up and down by the action of the lifting motor 112. Both the injection needle and the aspiration needle are provided on the lifting plate 111.

[0095] An upper position sensor 22 and a lower position sensor 23 are also provided on the top surface of the cleaning disc cylinder 9. These sensors are mounted on the top surface of the cleaning disc cylinder 9 via a support rod 24. A sensing plate 25 is also provided on the lifting plate 111. The sensing plate 25, in conjunction with the upper and lower position sensors 22 and 23, can detect the upper and lower limits of the lifting plate 111. During operation, when the lifting plate 111 moves to the upper limit position, it indicates that the injection needle and suction needle on the lifting plate 111 have moved into position and have been withdrawn from the reaction cup. At this time, the turntable can drive the reaction cup to rotate. When the lifting plate 111 descends to the lower limit position, it indicates that the suction needle on the lifting plate 111 has been inserted into the liquid surface of the reaction cup, and the suction operation can be performed.

[0096] like Figure 12 andFigure 13 As shown, the injection needle 7 and injection needle 8 are connected to the lifting plate 111 by a limiting block. The limiting block includes a seat 26 locked to the lifting plate 111 by screws and a screw cap 27 threaded to the seat 26. The seat 26 includes a base plate 261 and a column 262 disposed on the base plate 261. The column 262 and the base plate 261 are connected by a mounting through hole 263. A groove 28 is provided on the inner circumferential surface of the column 262 through the mounting through hole 263. The groove 28 is radially recessed along the column 262, and one side of the groove 28 is open, while the other three sides are closed. Figure 14 and Figure 15 As shown, a screw cap through hole 271 is opened at the bottom of the screw cap 27. A guide block 29 is also provided on the injection needle 7 and injection needle 8. The guide block 29 is cylindrical, and its diameter matches the diameter of the mounting through hole 263. A radially protruding protrusion 291 is provided on one side of the guide block 29. During installation, the injection needle 7 and injection needle 8 are sequentially passed through the screw cap through hole 271 of the screw cap 27, the guide block 29, and the mounting through hole 263 of the seat 26, so that the protrusion 291 of the guide block 29 is engaged in the groove 28. The groove 28 and the protrusion 291 cooperate to limit the injection needle 7 and injection needle 8 circumferentially. Then, the screw cap 27 is tightened onto the column 262, and the screw cap 27 presses the protrusion 291 into the groove 28, thereby limiting the injection needle 7 and injection needle 8 axially. Through the above structural design, the injection needle 7 and the injection needle 8 are connected to the lifting plate 111 and their positions are fixed, ensuring the normal operation of the injection work.

[0097] Example 2: The cleaning method in this example differs from that in Example 1 in that:

[0098] The locations of basic cleaning unit 1 and basic cleaning unit 2 overlap:

[0099] like Figure 16 As shown, multiple basic cleaning units are located at the same position above the turntable 3. Each basic cleaning unit has an injection station 4 and an aspiration station 5. The aspiration station 5 has an aspiration needle 6, and the injection station 4 has two injection needles. The two injection needles are integrated together and are called injection needle 1 7 and injection needle 2 8.

[0100] During cleaning, the turntable 3 drives the reaction cup (not shown in the figure) to rotate, so that the reaction cup passes through the basic cleaning unit in sequence, thereby completing the cleaning work. Let the number of rotations of the reaction cup driven by the turntable 3 be N2, then N2≥2.

[0101] The cleaning process includes cleaning steps for the remaining D1 turns and cleaning steps for the final D2 turn. The cleaning steps for the remaining D1 turns include:

[0102] 1) Control turntable 3 to drive the reaction cup (not shown in the figure) to rotate to the liquid injection station 4 in the basic cleaning unit, and then stop rotating;

[0103] (ii) Control the injection needle 7 in the injection station 4 of the basic cleaning unit to descend and extend into the reaction cup, inject cleaning solution A into the reaction cup, and use the injected cleaning solution A to clean the magnetic beads in the reaction cup; after the injection is completed, control the injection needle 7 in the injection station 4 of the basic cleaning unit to rise back to its original position.

[0104] 3) Control the turntable 3 to drive the reaction cup (not shown in the figure) to rotate to the liquid suction station 5 in the basic cleaning unit, and then stop rotating;

[0105] (iv) Control the suction needle 6 in the suction station 5 of the basic cleaning unit to descend and extend into the reaction cup to suction and discharge the cleaning liquid in the reaction cup; after discharge, control the suction needle 6 in the suction station 5 of the basic cleaning unit to rise back to its original position.

[0106] The above steps complete the cleaning of the other rings of D1.

[0107] The difference between the final cleaning cycle of D2 and the other cleaning cycles of D1 is as follows:

[0108] (ii) Control the injection needle 28 in the injection station 4 of the basic cleaning unit to descend and extend into the reaction cup, inject cleaning solution B into the reaction cup, and use the injected cleaning solution B to clean the magnetic beads in the reaction cup; after the injection is completed, control the injection needle 28 in the injection station 4 of the basic cleaning unit to rise back to its original position.

[0109] In summary, this invention utilizes two different cleaning solutions in combination to effectively clean the magnetic beads in the reaction vessel while simultaneously removing residual air bubbles, thus ensuring the accuracy of the final test results. By designing specific steps for injecting the two cleaning solutions sequentially through two injection needles at different cleaning stages, the removal of residual air bubbles is achieved. Furthermore, by designing a cleaning disc, the aforementioned cleaning method for the reaction vessel is implemented, effectively removing residual air bubbles and ensuring the accuracy of the final test results.

[0110] In this embodiment, "multiple" refers to "two or more". The above embodiments are for illustrative purposes only and are not intended to limit the invention. Those skilled in the art can make various changes or modifications without departing from the spirit and scope of the invention. Therefore, all equivalent technical solutions should also fall within the protection scope of this invention, which is defined by the claims.

Claims

1. A method for cleaning reaction cups of an immunoassay analyzer, characterized in that: During the cleaning process, two different cleaning solutions are used. One cleaning solution is used to clean the reaction cup first, and then the other cleaning solution is used to remove the residual air bubbles in the reaction cup before the reaction cup is sent to the next process for testing. The reaction cup is set as a basic cleaning unit by injecting liquid into the reaction cup and aspirating liquid into the reaction cup. In the reaction cup cleaning, the number of the basic cleaning units is set as N1, then N1≥2; among the N1 basic cleaning units, the basic cleaning unit before detection, that is, the last basic cleaning unit, is set as basic cleaning unit two (2), and the other basic cleaning units are set as basic cleaning unit one (1). Two cleaning liquids are used in the whole cleaning process, namely cleaning liquid A and cleaning liquid B. In the liquid injection step of basic cleaning unit one (1), cleaning liquid A is injected into the reaction cup and the reaction cup is cleaned with the cleaning liquid A. In the liquid injection step of basic cleaning unit two (2), cleaning liquid B is injected into the reaction cup and the residual air bubbles in the reaction cup are removed with the cleaning liquid B. Place the reaction cup on the turntable (3) and set the basic cleaning unit above the turntable (3); The distribution positions of the basic cleaning unit one (1) and the basic cleaning unit two (2) do not overlap. The basic cleaning unit one (1) and the basic cleaning unit two (2) are distributed sequentially along the circumference of the turntable (3). Each cleaning unit is provided with an injection station (4) and a suction station (5). Each suction station (5) is provided with a suction needle (6). The injection station (4) of the basic cleaning unit one (1) is provided with an injection needle one (7). The injection station (4) of the basic cleaning unit two (2) is provided with two injection needles, namely injection needle one (7) and injection needle two (8). During cleaning, the turntable (3) drives the reaction cup to rotate, so that the reaction cup passes through the basic cleaning unit, thereby completing the cleaning work; let the number of rotations of the reaction cup driven by the turntable (3) be N2, then N2≥2.

2. The reaction vessel cleaning method according to claim 1, characterized in that: When N2 = 2 cycles, the cleaning steps include a first cycle cleaning step S1 and a second cycle cleaning step S2. The first cycle cleaning step S1 includes: 1) Control the turntable (3) to drive the reaction cup to rotate to the liquid injection station (4) in the first basic cleaning unit (1), and stop rotating; 2) Control the injection needle (7) in the injection station (4) of the first basic cleaning unit (1) to descend and extend into the reaction cup, inject cleaning solution A into the reaction cup, and use the injected cleaning solution A to clean the reaction cup; after the injection is completed, control the injection needle (7) in the injection station (4) of the first basic cleaning unit (1) to rise back to its original position. 3) Control the turntable (3) to drive the reaction cup to rotate to the liquid suction station (5) in the first basic cleaning unit (1), and stop rotating; (iv) Control the liquid suction needle (6) in the liquid suction station (5) in the first basic cleaning unit (1) to descend and extend into the reaction cup to suck away and discharge the cleaning liquid in the reaction cup; after discharge, control the liquid suction needle (6) in the liquid suction station (5) in the first basic cleaning unit (1) to rise back to its original position. Then, follow steps one through four to operate the next basic cleaning unit (1) until all basic cleaning units (1) in the first cycle have been operated. Continue by following these steps: 5) Control the turntable (3) to drive the reaction cup to rotate to the liquid injection station (4) in the basic cleaning unit 2 (2), and stop rotating; (vi) Control the injection needle (7) in the injection station (4) of the basic cleaning unit (2) to descend and extend into the reaction cup, inject cleaning solution A into the reaction cup, and use the injected cleaning solution A to clean the reaction cup; after the injection is completed, control the injection needle (7) in the injection station (4) of the basic cleaning unit (2) to rise back to its original position. 7) Control the turntable (3) to drive the reaction cup to rotate to the liquid suction station (5) in the basic cleaning unit 2 (2), and stop rotating; 8) Control the liquid suction needle (6) in the liquid suction station (5) in the basic cleaning unit 2 (2) to descend and extend into the reaction cup to suck away and discharge the cleaning liquid in the reaction cup; after the discharge is completed, control the liquid suction needle (6) in the liquid suction station (5) in the basic cleaning unit 2 (2) to rise back to its original position; The first cleaning step of S1 is completed by the above steps. The difference between the second cleaning cycle S2 and the first cleaning cycle S1 lies in step six; all other steps are the same as the first cleaning cycle S1. (vi) Control the injection needle (8) in the injection station (4) of the basic cleaning unit (2) to descend and extend into the reaction cup, inject cleaning solution B into the reaction cup, and use the injected cleaning solution B to clean the reaction cup; after the injection is completed, control the injection needle (8) in the injection station (4) of the basic cleaning unit (2) to rise back to its original position.

3. The reaction vessel cleaning method according to claim 2, characterized in that: When N2 > 2 cycles, the cleaning steps include the T1 other cycle cleaning step and the T2 last cycle cleaning step. The T1 other cycle cleaning step is the same as the S1 first cycle cleaning step, and the T2 last cycle cleaning step is the same as the S2 second cycle cleaning step.

4. A method for cleaning reaction cups of an immunoassay analyzer, characterized in that: During the cleaning process, two different cleaning solutions are used. One cleaning solution is used to clean the reaction cup first, and then the other cleaning solution is used to remove the residual air bubbles in the reaction cup before the reaction cup is sent to the next process for testing. The reaction cup is set as a basic cleaning unit by injecting liquid into the reaction cup and aspirating liquid into the reaction cup. In the reaction cup cleaning, the number of the basic cleaning units is set as N1, then N1≥2; among the N1 basic cleaning units, the basic cleaning unit before detection, that is, the last basic cleaning unit, is set as basic cleaning unit two (2), and the other basic cleaning units are set as basic cleaning unit one (1). Two cleaning liquids are used in the whole cleaning process, namely cleaning liquid A and cleaning liquid B. In the liquid injection step of basic cleaning unit one (1), cleaning liquid A is injected into the reaction cup and the reaction cup is cleaned with the cleaning liquid A. In the liquid injection step of basic cleaning unit two (2), cleaning liquid B is injected into the reaction cup and the residual air bubbles in the reaction cup are removed with the cleaning liquid B. Place the reaction cup on the turntable (3) and set the basic cleaning unit above the turntable (3); The distribution of the basic cleaning unit one (1) and the basic cleaning unit two (2) overlaps. Multiple basic cleaning units are located at the same position above the turntable (3). Each basic cleaning unit is equipped with an injection station (4) and an aspiration station (5). A suction needle (6) is provided in the aspiration station (5), and two injection needles are provided in the injection station (4), namely injection needle one (7) and injection needle two (8). During cleaning, the turntable (3) drives the reaction cup to rotate, so that the reaction cup passes through the basic cleaning unit in sequence, thereby completing the cleaning work; let the number of rotations of the reaction cup driven by the turntable (3) be N2, then N2≥2.

5. The reaction vessel cleaning method according to claim 4, characterized in that: The cleaning process includes cleaning steps for the remaining D1 turns and cleaning steps for the final D2 turn. The cleaning steps for the remaining D1 turns include: 1) Control the turntable (3) to drive the reaction cup to rotate to the liquid injection station (4) in the basic cleaning unit, and then stop rotating; 2) Control the injection needle (7) in the injection station (4) of the basic cleaning unit to descend and extend into the reaction cup, inject cleaning solution A into the reaction cup, and use the injected cleaning solution A to clean the reaction cup; after the injection is completed, control the injection needle (7) in the injection station (4) of the basic cleaning unit to rise back to its original position. 3) Control the turntable (3) to drive the reaction cup to rotate to the liquid suction station (5) in the basic cleaning unit, and stop rotating; (iv) Control the suction needle (6) in the suction station (5) of the basic cleaning unit to descend and extend into the reaction cup to suction out the cleaning liquid in the reaction cup; after the discharge is completed, control the suction needle (6) in the suction station (5) of the basic cleaning unit to rise back to its original position. The cleaning steps for the other rings of D1 are completed through the above steps; The difference between the final cleaning cycle of D2 and the other cleaning cycles of D1 lies in step two, namely: 2) Control the injection needle 2 (8) in the injection station (4) of the basic cleaning unit to descend and extend into the reaction cup, inject cleaning solution B into the reaction cup, and use the injected cleaning solution B to clean the reaction cup; after the injection is completed, control the injection needle 2 (8) in the injection station (4) of the basic cleaning unit to rise back to its original position.

6. A cleaning disc, characterized in that: The system includes a cleaning disc cylinder (9), a turntable mechanism (10) located inside the cleaning disc cylinder (9), and a needle lifting mechanism (11) located above the cleaning disc cylinder (9). The reaction cup is placed on the turntable (3) of the turntable mechanism (10). The turntable (3) can drive the reaction cup to rotate. Along the circumference of the turntable (3), two or more basic cleaning units are sequentially arranged on the needle lifting mechanism (11). The basic cleaning unit includes a basic cleaning unit one and a basic cleaning unit two. Each basic cleaning unit one is provided with an injection needle one (7) and an aspiration needle (6). The basic cleaning unit two is provided with an integrated injection double needle (S) and an aspiration needle (6). The integrated injection double needle (S) includes an injection needle one (7) and an injection needle two (8). Driven by the downward movement of the needle body lifting mechanism (11), both the injection needle and the aspiration needle can be lowered and inserted into the reaction cup to perform injection and aspiration operations. Driven by the upward movement of the needle body lifting mechanism (11), both the injection needle and the aspiration needle can be pulled out of the reaction cup and returned to their original positions. The integrated injection double needle (S) is connected to the lifting plate (111) of the needle body lifting mechanism (11) by a limiting block. The limiting block includes a seat (26) set on the lifting plate (111) and a screw cap (27) threadedly connected to the seat (26). The seat (26) includes a base plate (261) and a column (262) set on the base plate (261). The column (262) and the base plate (261) are connected by a mounting through hole (263). A groove (28) is provided on the inner circumferential surface of the column (262) through which the mounting through hole (263) is connected. The groove (28) is radially recessed along the column (262) and one side of the groove (28) is open and the other three sides are closed. A screw cap through hole (271) is opened at the bottom of the screw cap (27). A guide block (29) is also provided on the integrated injection double needle (S). The guide block (29) is cylindrical and its diameter matches the diameter of the mounting through hole (263). A radially protruding protrusion (291) is provided on one side of the guide block (29). During installation, the injection needle one (7) and the injection needle two (8) are passed through the screw cap through hole (271), the guide block (29), and the seat (263) of the screw cap (27) in sequence. The mounting through hole (263) allows the protrusion (291) of the guide block (29) to be inserted into the groove (28). The groove (28) and the protrusion (291) cooperate to limit the injection needle one (7) and injection needle two (8) in the circumferential direction. Then, the screw cap (27) is tightened on the column (262). The screw cap (27) presses the protrusion (291) into the groove (28), thereby limiting the injection needle one (7) and injection needle two (8) in the axial direction.

7. The cleaning tray according to claim 6, characterized in that: The needle lifting mechanism (11) also includes a lifting motor (112). A guide sleeve (18) is provided on the lifting plate (111). A guide rod (19) is provided on the top surface of the cleaning disc cylinder (9). The lifting plate (111) is slidably connected to the guide rod (19) by the cooperation between the guide rod (19) and the guide sleeve (18). The lifting motor (112) is provided on the cleaning disc cylinder (9). Its output shaft is connected to the lead screw (20) for transmission. A nut sleeve (21) is provided on the lifting plate (111). The lead screw (20) and the nut sleeve (21) cooperate to form a lead screw and nut mechanism. The lifting plate (111) can be moved up and down by the action of the lifting motor (112). The injection needle and the suction needle are both provided on the lifting plate (111).

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