Dental chair water pipe material with antibacterial hydrogel coating, preparation method and use method

Abstract

The present application discloses a dental chair water pipe material with an antibacterial hydrogel coating, as well as a preparation method and a method of use. The present invention soaks the polyurethane pipe material in a benzophenone solution with a photoinitiator function, and forms a sewage-proof gel layer on its surface by cross-linking sulfobetaine methacrylate and hydroxyethyl methacrylate through photoinitiation; then, pyridinium chlorochromate is used to hydroformylate the hydroxyl group of HEMA, and through a Schiff base reaction, antimicrobial peptides are loaded in the hydrogel layer. The present invention utilizes the reversibility of the Schiff base bond to release antimicrobial peptides in the microenvironment where bacteria produce acid, thereby releasing a bactericidal effect. In addition, the present invention can be combined with the flushing and disinfection of dental chair water pipes in clinical practice, using hypochlorous acid to flush the pipes to remove bacteria and antimicrobial peptides that have already taken effect; then, the active antimicrobial peptides can be reloaded through soaking to achieve regeneration of the antimicrobial agent and achieve long-lasting antimicrobial performance, providing a new strategy for the prevention and control of dental chair water pollution.

Description

Technical Field

[0001] The present application relates to the technical field of dental chair waterway pollution prevention and control, and in particular to a dental chair waterway pipeline material with an antibacterial hydrogel coating, a preparation method, and a use method. Background Art

[0002] The dental water system refers to the system consisting of the water supply pipes, drainage pipes, and independent water storage tanks used by dental chairs. The dental water system consists of narrow, slender pipes that are difficult to clean and disinfect, and are easily contaminated by bacteria, forming biofilms that continue to accumulate. Currently, the main measures used to prevent and control dental water system pollution are physical disinfection and chemical disinfection. Physical disinfection reduces effluent contamination by improving water supply quality, draining and drying water lines, flushing, using anti-backflow valves and filters, but physical methods have little effect on biofilms that have already formed in the pipes. Chemical disinfection uses alkaline peroxides, sodium perborate / EDTA, hydrogen peroxide, chlorhexidine, hypochlorous acid, and other agents to achieve a bactericidal effect. However, chemical disinfectants may have adverse effects such as corrosion of dental chair components, clogging of pipes, and the induction of microbial resistance. Summary of the Invention

[0003] To address the technical deficiencies of existing dental chair waterway pollution control measures, this application provides a dental chair waterway pipe material with an antibacterial hydrogel coating, as well as a preparation method and use method. This hydrogel coating possesses long-lasting antibacterial properties, providing a new strategy for dental chair waterway pollution control in a simple and convenient manner.

[0004] In a first aspect, the present invention provides a method for preparing a dental chair water pipe material having an antibacterial hydrogel coating, which is achieved by adopting the following technical solutions.

[0005] A method for preparing a dental chair water channel pipe material with an antibacterial hydrogel coating comprises the following steps:

[0006] S1. The polyurethane pipe material is immersed in an acetone solution containing 5-20wt% benzophenone, soaked for a period of time, washed and dried;

[0007] S2. The product obtained in step S1 is immersed in water containing 0.01-10wt% sulfobetaine methacrylate monomer and 0.01-20wt% hydroxyethyl methacrylate monomer, washed and dried after ultraviolet irradiation;

[0008] S3. The product obtained in step S2 is immersed in water containing pyridinium chlorochromate, stirred, washed, and dried;

[0009] S4. Immerse the product obtained in step S3 in PBS containing antimicrobial peptides, stir, wash, and dry.

[0010] Preferably, in step S1, the concentration of the acetone solution containing benzophenone is 10 wt%.

[0011] Furthermore, in step S1, the polyurethane pipe material is immersed in the benzophenone solution for 5-10 minutes.

[0012] Preferably, in step S2, the concentration of the methacryloylethyl sulfobetaine monomer is 5 wt%; and the concentration of the hydroxyethyl methacrylate monomer is 5 wt%.

[0013] Furthermore, in step S2, the ultraviolet wavelength is 365 nm, and the irradiation time is 0.25-1.5 h, preferably 0.5 h.

[0014] Furthermore, in step S3, the concentration of pyridinium chlorochromate is 5-15 mg / mL, preferably 10 mg / mL.

[0015] Furthermore, in step S3, the stirring condition is: stirring at 30° C. for 6-10 h, preferably 6 h.

[0016] Furthermore, in step S4, the antimicrobial peptide is an antimicrobial peptide containing an amino group, including polylysine antimicrobial peptides and antimicrobial peptide Jelleine-1 analog 15; the concentration of the polylysine antimicrobial peptide is 25-75 mg / mL, preferably 50 mg / mL; the concentration of the antimicrobial peptide Jelleine-1 analog 15 is 5-10 mg / mL, preferably 10 mg / mL.

[0017] Furthermore, in step S4, the stirring condition is: stirring at 30° C. for 12-24 hours, preferably 24 hours.

[0018] In a second aspect, the present invention provides a dental chair water pipe material with an antibacterial hydrogel coating, which is achieved by adopting the following technical solutions.

[0019] A dental chair water channel pipe material with an antibacterial hydrogel coating prepared by the above method.

[0020] In a third aspect, the present invention provides a method for using a dental chair water pipe material having an antibacterial hydrogel coating, which is achieved by adopting the following technical solution.

[0021] A method for using the aforementioned dental chair water channel material with an antimicrobial hydrogel coating is described. In a microenvironment where bacteria produce acid, the dental chair water channel material with the antimicrobial hydrogel coating responds to the acidic environment and releases antimicrobial peptides, achieving a bactericidal effect. Furthermore, referring to the timed flushing of dental chair water channels in clinical practice, the dental chair water channel material with the antimicrobial hydrogel coating is immersed in a hypochlorous acid solution, stirred, washed, and dried to remove bacteria and active antimicrobial peptides.

[0022] Furthermore, the material after releasing the antimicrobial peptides is immersed in PBS containing the antimicrobial peptides again, stirred, washed, and dried to achieve regeneration of the antimicrobial property.

[0023] This application has the following beneficial effects.

[0024] This invention creates a simple and convenient antimicrobial hydrogel coating on the surface of dental chair water pipes. This coating responds to the acidic bacterial microenvironment, releasing antimicrobial peptides to kill free bacteria. The coating also provides an antifouling function, preventing bacterial adhesion. Furthermore, by re-immersing the material in antimicrobial peptides, the antimicrobial agent can be regenerated, achieving long-lasting antimicrobial properties. This provides a new strategy for preventing and controlling dental chair water pipe contamination. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 1 is a scanning electron microscope image of the surface of PU (a), PPU (b), PPU-CHO (c), and PAPU (d) in Example 1 of the present invention;

[0026] Figure 2 1 is a scanning electron microscope image of the surface of PU (a), PPU (b), PPU-CHO (c), and PAPU (d) in Example 2 of the present invention;

[0027] Figure 3 1 is a scanning electron microscope image of the surface of PU (a), PPU (b), PPU-CHO (c), and PAPU (d) in Example 3 of the present invention;

[0028] Figure 4 1 is a scanning electron microscope (SEM) image of the longitudinal section of the PU of the present invention (a), the PAPU in Example 1 (b), the PAPU in Example 2 (c), and the PAPU in Example 3 (d);

[0029] Figure 5 These are confocal laser scanning microscopy (CLSM) images of Pseudomonas aeruginosa cultured on the surfaces of PPU-CHO (a) and PAPU (b) for 10 hours, followed by SYTO 9 / PI live / dead bacterial double staining in Example 1 of the present invention;

[0030] Figure 6 These are confocal laser scanning microscopy (CLSM) images of Pseudomonas aeruginosa cultured on the surfaces of PPU-CHO (a) and PAPU (b) for 10 hours, followed by SYTO 9 / PI live / dead bacterial double staining in Example 2 of the present invention;

[0031] Figure 7 These are confocal laser scanning microscopy (CLSM) images of Pseudomonas aeruginosa cultured on the surfaces of PPU-CHO (a) and PAPU (b) for 10 hours, followed by SYTO 9 / PI live / dead bacterial double staining in Example 3 of the present invention. DETAILED DESCRIPTION

[0032] The present patent application is further described below with reference to the embodiments.

[0033] Unless otherwise specified, the experimental methods used in the following examples are conventional methods; the materials, reagents, etc. used in the following preparation examples and examples are all commercially available unless otherwise specified.

[0034] The sulfobetaine methacrylate (SBMA) used in the following examples of the present invention was purchased from Shanghai MacLean Biochemical Technology Co., Ltd.; CAS number: 3637-26-1;

[0035] The hydroxyethyl methacrylate (HEMA) used in the following examples of the present invention was purchased from Shanghai Aladdin Biochemical Technology Co., Ltd.; CAS No.: 868-77-9;

[0036] Pyridinium chlorochromate (PCC) used in the following examples of the present invention was purchased from Shanghai MacLean Biochemical Technology Co., Ltd.; CAS No.: 26299-14-9.

[0037] Example 1

[0038] A method for preparing a dental chair water channel pipe material with an antibacterial hydrogel coating comprises the following steps:

[0039] (1) Formation of BP-PU mixed layer: A 0.5 cm*0.5 cm polyurethane pipe material (PU) was ultrasonically cleaned with anhydrous ethanol for 5 min at room temperature. After drying, it was immersed in an acetone solution containing 10 wt% benzophenone (BP) for 5 min, then gently rinsed with ddH2O and ethanol alternately and dried.

[0040] (2) Synthesis of a cross-linked anti-fouling gel layer (PPU) of sulfobetaine methacrylate (SBMA) and hydroxyethyl methacrylate (HEMA): The synthesized BP-PU was immersed in ddH2O containing 5 wt% SBMA monomer and 5 wt% HEMA monomer at room temperature and irradiated with ultraviolet light (365 nm) for 30 min. It was then gently rinsed with ddH2O and dried.

[0041] (3) Synthesis of hydroformylation anti-fouling gel layer (PPU-CHO): The synthesized PPU was immersed in ddH2O containing 10 mg / mL pyridinium chlorochromate (PCC), stirred at 30 °C for 6 h, and finally rinsed alternately with ddH2O and ethanol and dried naturally.

[0042] (4) Synthesis of antimicrobial anti-fouling gel (PAPU) with the introduction of antimicrobial peptides: The PPU-CHO synthesized above was immersed in PBS containing 50 mg / mL polylysine, stirred at 30°C for 24 h, and finally washed alternately with ddH2O and ethanol three times and dried naturally.

[0043] (5) Release of antimicrobial peptides from the hydrogel: PAPU was immersed in 3 mL of hypochlorous acid solution, stirred at 30 °C for 2 h, washed alternately in ddH2O and ethanol three times, and dried naturally to cleave the Schiff base bonds and release the antimicrobial peptides.

[0044] (6) Synthesis of antimicrobial anti-pollution gel (PAPU) with reintroduction of antimicrobial peptides: The PPU-CHO after releasing antimicrobial peptides was re-immersed in PBS containing 50 mg / mL polylysine, stirred at 30 °C for 24 h, and finally washed alternately with ddH2O and ethanol three times and dried naturally.

[0045] The surface scanning electron microscopy images of PU, PPU, PPU-CHO and PAPU prepared in this example are shown in Figure 1 ; The longitudinal section scanning electron microscope images of PU and PAPU are shown in Figure 4 .

[0046] Example 2

[0047] A method for preparing a dental chair water pipe material with an antibacterial hydrogel coating is different from that of Example 1 in that: in step (2), the ultraviolet (365nm) irradiation time is 15 minutes.

[0048] The surface scanning electron microscopy images of PU, PPU, PPU-CHO and PAPU prepared in this example are shown in Figure 2 ; The longitudinal section scanning electron microscope images of PU and PAPU are shown in Figure 4 .

[0049] Example 3

[0050] A method for preparing a dental chair water pipe material with an antibacterial hydrogel coating, which differs from Example 1 in that: in step (2), the above-synthesized BP-PU is immersed in ddH2O containing 5wt% SBMA monomer and 10wt% HEMA monomer, and the ultraviolet (365nm) irradiation time is 15min.

[0051] The surface scanning electron microscopy images of PU, PPU, PPU-CHO and PAPU prepared in this example are shown in Figure 3 ; The longitudinal section scanning electron microscope images of PU and PAPU are shown in Figure 4 .

[0052] Performance testing

[0053] Use LB liquid medium to expand the culture of Pseudomonas aeruginosa (P. aeruginosa), and dilute the P. aeruginosa bacterial suspension to a density of 10 8 CFU / mL. Add 0.5 cm x 0.5 cm of PPU-CHO or PAPU to a 48-well plate, then add 400 μL of P. aeruginosa bacterial suspension to each well. Incubate at 37°C for 10 hours. After 10 hours of incubation, gently wash the sample three times with PBS to remove unadhered bacteria. Then, stain with live-dead staining and observe with CLSM.

[0054] like Figure 5 As shown in a, 6a, and 7a, a small amount of live bacteria can be seen adhering to the surface of the PPU-CHO sample, and almost no dead bacteria are seen. Figure 5 As shown in Figures b, 6b, and 7b, a large number of dead bacteria were observed on the surface of the PAPU sample, with almost no live bacteria. Comparing Figures a and b, it can be seen that the introduction of antimicrobial peptides (polylysine) into the hydrogel network formed by cross-linking SBMA and HEMA can impart strong antifouling and antibacterial properties to the hydrogel.

[0055] The embodiments of this specific implementation method are all preferred embodiments of the present application and are not intended to limit the scope of protection of the present application. Therefore, any equivalent changes made based on the structure, shape, and principle of the present application should be included in the scope of protection of the present application.

Claims

1. A method for preparing a dental chair water pipe material with an antibacterial hydrogel coating, characterized by: The following steps are involved: S1. The polyurethane pipe material is immersed in an acetone solution containing 5-20wt% benzophenone, soaked for a period of time, washed and dried; S2. The product obtained in step S1 is immersed in water containing 0.01-10wt% sulfobetaine methacrylate monomer and 0.01-20wt% hydroxyethyl methacrylate monomer, washed and dried after ultraviolet irradiation; S3. The product obtained in step S2 is immersed in water containing pyridinium chlorochromate, stirred, washed, and dried; S4. Immerse the product obtained in step S3 in PBS containing an antimicrobial peptide, stir, wash, and dry; the antimicrobial peptide is an antimicrobial peptide containing an amino group.

2. The method for preparing a dental chair water pipe material with an antibacterial hydrogel coating according to claim 1, characterized in that: In step S1, the polyurethane pipe material is immersed in the benzophenone solution for 5-10 minutes.

3. The method for preparing a dental chair water pipe material with an antibacterial hydrogel coating according to claim 1, characterized in that: In step S2, the ultraviolet wavelength is 365 nm, and the irradiation time is 0.25-1.5 h.

4. The method for preparing a dental chair water pipe material with an antibacterial hydrogel coating according to claim 1, characterized in that: In step S3, the concentration of pyridinium chlorochromate is 5-15 mg / mL.

5. The method for preparing a dental chair water pipe material with an antibacterial hydrogel coating according to claim 1, characterized in that: In step S3, the stirring condition is: stirring at 30° C. for 6-10 h.

6. The method for preparing a dental chair water pipe material with an antibacterial hydrogel coating according to claim 1, characterized in that: In step S4, the antimicrobial peptide containing an amino group includes polylysine antimicrobial peptides and antimicrobial peptide Jelleine-1 analog 15; the concentration of the polylysine antimicrobial peptide is 25-75 mg / mL, and the concentration of the antimicrobial peptide Jelleine-1 analog 15 is 5-10 mg / mL.

7. The method for preparing a dental chair water pipe material with an antibacterial hydrogel coating according to claim 1, characterized in that: In step S4, the stirring condition is: stirring at 30° C. for 12-24 hours.

8. A dental chair water pipe material with an antibacterial hydrogel coating prepared by the method according to any one of claims 1 to 7.

9. A method for using the dental chair water pipe material with an antibacterial hydrogel coating according to claim 8, characterized in that: In the microenvironment where bacteria produce acid, the dental chair water channel pipe material with an antibacterial hydrogel coating responds to the acidic environment, releases antimicrobial peptides, and achieves a bactericidal effect; referring to the timed flushing of the dental chair water channel in clinical operations, the dental chair water channel pipe material with an antibacterial hydrogel coating is immersed in a hypochlorous acid solution, stirred, washed, and dried to remove bacteria and antimicrobial peptides that have taken effect.

10. A method for using the dental chair water pipe material with an antibacterial hydrogel coating according to claim 9, characterized in that: The material after releasing the antimicrobial peptides is re-immersed in PBS containing the antimicrobial peptides, stirred, washed, and dried to achieve regeneration of the antimicrobial property.

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