Preparation method of lotus root extract, application thereof in cosmetics and moisturizing water

Abstract

The application discloses a preparation method of lotus root extract, application of the lotus root extract in cosmetics and moisturizing water, and belongs to the technical field of cosmetics. The preparation method mainly comprises the following steps: sterilizing and adjusting the PH value of lotus root pulp, then performing enzymolysis on the lotus root pulp, filtering to obtain an enzymolysis liquid, performing fermentation on the enzymolysis liquid to obtain a fermentation liquid, filtering the fermentation liquid to obtain a lotus root extract liquid, and then performing low-temperature drying to prepare the lotus root extract. The moisturizing water is mainly prepared by mixing the following components in proportion by weight: 1-20 parts of the lotus root extract, 1-10 parts of glycerol, 1-10 parts of hyaluronic acid, 1-10 parts of tremella polysaccharide and the rest of ultrapure water. The moisturizing water has good moisturizing effect, and can make the skin keep a state of being moist and fresh for a long time and not being tight. The preparation method of the lotus root extract has the advantages of simple process, convenience in preparation, high production efficiency, good product quality, low starch content, low viscosity, strong moisturizing performance and low production cost.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of cosmetics, and particularly relates to a preparation method of lotus root extract, application of the lotus root extract in cosmetics, and moisturizing water. BACKGROUND

[0002] With the improvement of living and cultural levels, cosmetics are paid more and more attention by people. Cosmetics containing natural active ingredients are favored by more and more people due to good efficacy, small side effects, and more safety. Research and development of natural active ingredients applied in cosmetics become an important link for sustainable development of the cosmetic industry. For example, natural plant extracts. More than 100 natural plant extracts have been used in cosmetics in the United States and Japan, such as natural plant oils (perfume, skin care), aloe vera extract (bactericidal, whitening), green tea extract (antioxidant, anti-aging), ginkgo biloba extract (clearing oxygen free radicals), grape seed extract (clearing oxygen free radicals), wheat extract (anti-aging), seaweed extract (clearing free radicals, anti-aging, moisturizing, sunscreen, weight loss), etc. Currently, no one has applied the ingredients in lotus root to cosmetics. SUMMARY

[0003] The purpose of the embodiment of the present application is to provide a preparation method of lotus root extract, which has simple process, convenient production, high production efficiency, and good product quality.

[0004] Another purpose of the embodiment of the present application is to provide a moisturizing water, which has good moisturizing effect and can keep the skin in a state of long-term water and freshness without tightness.

[0005] The embodiment of the present application is implemented as follows:

[0006] The embodiment of the present application provides a preparation method of lotus root extract, comprising the following steps:

[0007] S1: lotus root is made into lotus root powder;

[0008] S2: an appropriate amount of lotus root powder is taken, mixed with pure water to obtain lotus root paste, and then the lotus root paste is sterilized by using the pasteurization method;

[0009] S3: the PH value of the sterilized lotus root paste is adjusted by using a PH value adjusting agent, and the PH value is adjusted to 5.5-6.5;

[0010] S4: cellulase, pectinase, and amylase are added to the lotus root paste, and then the enzyme is hydrolyzed at 45-60 DEG C for 24-48 hours to obtain an enzyme hydrolysate;

[0011] S5: adopting the pasteurization method to sterilize the enzymatic hydrolysate, then standing to normal temperature, then adding the compound probiotics, stirring uniformly, placing in the condition of 36-38 DEG C to ferment for 12-72 hours, fermenting until the acidity reaches PH 4-5, obtaining the fermentation liquor;

[0012] S6: placing the fermentation liquor in the natural fermentation container, fermenting at normal temperature for at least 7 days, then filtering to obtain the lotus root extract;

[0013] S7: storing the lotus root extract after sterilization for later use, or low-temperature drying the lotus root extract to prepare the lotus root extract for storage.

[0014] Further, in the step S1, the sieved lotus root powder is sieved by using a 100-mesh sieve.

[0015] Further, in the step S2, the volume ratio of the amount of pure water to the amount of lotus root powder is 4-6:1.

[0016] Further, in the step S3, the PH value regulator is acetic acid.

[0017] Further, in the step S4, the weight ratio of lotus root paste, cellulase, pectinase and amylase is: lotus root paste 1000 parts, cellulase 6-8 parts, pectinase 6-12 parts, and amylase 2-5 parts.

[0018] Further, in the step S5, the enzymatic hydrolysate is sterilized at 85 DEG C for 15 minutes.

[0019] Further, in the step S5, the weight ratio of the amount of compound probiotics to the amount of enzymatic hydrolysate is 1-3:200.

[0020] Further, in the step S5, the compound probiotics are mainly prepared by mixing Lactobacillus plantarum, Bacillus subtilis and yeast according to the weight ratio of 1:1:1, and the total number of viable bacteria of the compound probiotics is greater than 5x10 8 CFU / g.

[0021] Further, in the step S7, the lotus root extract is sterilized at 40-60 DEG C for low-temperature sterilization for 20-35 minutes, and then sterilized at 105-135 DEG C for high-temperature sterilization for 15-30 seconds.

[0022] The embodiment of the application provides the application of the lotus root extract prepared by the preparation method of the lotus root extract in cosmetics, and the moisturizing property of the cosmetics can be greatly improved.

[0023] The embodiment of the application also provides a moisturizing water which is mainly prepared by mixing the following components in the weight ratio: 1-20 parts of the lotus root extract prepared by the preparation method, 1-10 parts of glycerol, 1-10 parts of 1 hyaluronic acid, 1-10 parts of tremella polysaccharide and the rest of ultrapure water.

[0024] The beneficial effects of the present application are:

[0025] The preparation method of lotus root extract provided by the embodiment of the present application has simple process, convenient preparation, high production efficiency, good product quality, low starch content, low viscosity, strong moisturizing performance, and low production cost.

[0026] The moisturizing water provided by the embodiment of the present application has good moisturizing effect, can make the skin keep a state of water and refreshing for a long time, and has wide market promotion and application value. DETAILED DESCRIPTION

[0027] To make the purpose, technical scheme and advantages of the embodiments of the present application clearer, the technical scheme in the embodiments of the present application is described clearly and completely below. Obviously, the described embodiments are part of the embodiments of the present application, rather than all the embodiments.

[0028] Example 1

[0029] The embodiment one of the present application provides a preparation method of lotus root extract, which mainly includes the following steps:

[0030] S1: The lotus root is made into lotus root powder, and a 100-mesh screen is used to screen the ground lotus root powder, and the screened lotus root powder is used.

[0031] S2: A proper amount of lotus root powder is taken, pure water is added and stirred to obtain lotus root paste, and then the lotus root paste is sterilized by using the pasteurization method.

[0032] In this embodiment, the volume ratio of the amount of pure water to the amount of lotus root powder is 4:1.

[0033] S3: The PH value of the sterilized lotus root paste is adjusted by using a PH value adjusting agent, and the PH value is adjusted to 5.5-6.5.

[0034] In this embodiment, acetic acid is used to adjust the PH value of the lotus root paste, and the PH value of the lotus root paste is adjusted to 5.5.

[0035] S4: Cellulase, pectinase and amylase are added to the lotus root paste, and then the enzyme hydrolysis is carried out at 45-60℃ for 24-48 hours to obtain an enzyme hydrolysis liquid.

[0036] In this embodiment, the weight ratio of the lotus root paste, cellulase, pectinase and amylase is: 1000 parts of lotus root paste, 6 parts of cellulase, 12 parts of pectinase and 5 parts of amylase. Then the enzyme hydrolysis is carried out at 45℃ for 48 hours.

[0037] S5: Pasteurization is used to sterilize the enzymatic hydrolysate, and then it is left to stand until room temperature, and then the compound probiotic bacteria are added, stirred uniformly, and placed in a 36-38℃ environment for fermentation for 12-72 hours until the acidity reaches a pH of 4-5 to obtain the fermentation liquor;

[0038] In this step, the enzymatic hydrolysate is sterilized at 85℃ for 15 minutes, and then left to stand and cool to room temperature before use.

[0039] In this embodiment, the amount of compound probiotic bacteria added is 1:200 by weight of the enzymatic hydrolysate.

[0040] In this embodiment, the compound probiotic bacteria are mainly made by mixing Lactobacillus plantarum, Bacillus subtilis, and yeast in a weight ratio of 1:1:1, and the total number of viable bacteria of the compound probiotic bacteria is greater than 5x10 8 CFU / g.

[0041] S6: The fermentation liquor is placed in a natural fermentation container and fermented at room temperature for at least 7 days, and then filtered to obtain lotus root extract;

[0042] S7: The lotus root extract is sterilized and stored for later use, or the lotus root extract is dried at low temperature to prepare lotus root extract for storage.

[0043] It should be noted that the lotus root extract can be directly used in cosmetics after sterilization, and the sterilization conditions of the lotus root extract are low-temperature sterilization at 40℃ for 35 minutes, and then high-temperature sterilization at 135℃ for 15 seconds.

[0044] Example 2

[0045] The embodiment two of the present application provides a preparation method of lotus root extract, which mainly comprises the following steps:

[0046] S1: The lotus root is made into lotus root powder, and a 100-mesh sieve is used to screen the ground lotus root powder, and the screened lotus root powder is used.

[0047] S2: An appropriate amount of lotus root powder is taken, pure water is added and stirred uniformly to prepare lotus root paste, and then the lotus root paste is sterilized by pasteurization;

[0048] In this embodiment, the volume ratio of the amount of pure water to the amount of lotus root powder is 5:1.

[0049] S3: The PH value of the sterilized lotus root paste is adjusted using a PH value adjusting agent, and the PH value is adjusted to 5.5-6.5;

[0050] In this embodiment, acetic acid is used to adjust the PH value of the lotus root paste, and the PH value of the lotus root paste is adjusted to 6.

[0051] S4: adding cellulase, pectinase and amylase into the lotus root paste, and then performing enzymolysis at 45-60℃ for 24-48 hours to obtain an enzymolysis solution by filtration;

[0052] In this embodiment, the weight ratio of the lotus root paste, cellulase, pectinase and amylase is: 1000 parts of lotus root paste, 7 parts of cellulase, 6 parts of pectinase and 3 parts of amylase. Then, the enzymolysis is performed at 50℃ for 35 hours.

[0053] S5: performing sterilization treatment on the enzymolysis solution by using the pasteurization method, and then standing until room temperature, adding the compound probiotics, stirring uniformly, and then placing in a fermentation tank to ferment at 36-38℃ for 12-72 hours until the acidity reaches PH 4-5 to obtain a fermentation solution;

[0054] In this step, the enzymolysis solution needs to be sterilized at 85℃ for 15 minutes, and then cooled to room temperature for standby.

[0055] In this embodiment, the weight ratio of the compound probiotics to the enzymolysis solution is 3:200.

[0056] In this embodiment, the compound probiotics are mainly prepared by mixing Lactobacillus plantarum, Bacillus subtilis and yeast according to a weight ratio of 1:1:1, and the total number of viable bacteria of the compound probiotics is greater than 5×10 8 CFU / g.

[0057] S6: placing the fermentation solution in a natural fermentation container to ferment at room temperature for at least 7 days, and then filtering to obtain a lotus root extract;

[0058] S7: storing the lotus root extract after sterilization for standby, or drying the lotus root extract at low temperature to prepare a lotus root extract for standby.

[0059] It should be noted that the lotus root extract after sterilization can be directly used in cosmetics. The sterilization condition of the lotus root extract is low-temperature sterilization at 60℃ for 20 minutes, and then high-temperature sterilization at 105℃ for 30 seconds.

[0060] Embodiment 3

[0061] The embodiment three of the present application provides a preparation method of a lotus root extract, which mainly comprises the following steps:

[0062] S1: grinding lotus root into lotus root powder, and then screening the ground lotus root powder by using a 100-mesh screen for standby.

[0063] S2: taking a proper amount of lotus root powder, adding purified water to stir uniformly to obtain lotus root paste, and then performing sterilization treatment on the lotus root paste by using the pasteurization method;

[0064] In this embodiment, the volume ratio of the amount of purified water to the amount of lotus root powder is 6:1.

[0065] S3: adjusting the PH value of the lotus root slurry after sterilization using a PH value regulator, and adjusting the PH value to 5.5-6.5;

[0066] In this embodiment, acetic acid is used to adjust the PH value of the lotus root slurry, and the PH value of the lotus root slurry is adjusted to 6.5.

[0067] S4: adding cellulase, pectinase and amylase to the lotus root slurry, and then enzymolysis at 45-60°C for 24-48 hours to obtain an enzymolysis solution by filtration;

[0068] In this embodiment, the weight ratio of lotus root slurry, cellulase, pectinase and amylase is: lotus root slurry 1000 parts, cellulase 7 parts, pectinase 6 parts, and amylase 3 parts. Then enzymolysis at 60°C for 24 hours.

[0069] S5: sterilizing the enzymolysis solution by the pasteurization method, then standing to room temperature, adding a compound probiotic, stirring uniformly, and placing at 36-38°C for fermentation for 12-72 hours until the acidity reaches PH 4-5 to obtain a fermentation solution;

[0070] In this step, the enzymolysis solution needs to be sterilized at 85°C for 15 minutes, and then cooled to room temperature before use.

[0071] In this embodiment, the weight ratio of the amount of compound probiotic added to the enzymolysis solution is 1:100.

[0072] In this embodiment, the compound probiotic is mainly made by mixing Lactobacillus plantarum, Bacillus subtilis and yeast at a weight ratio of 1:1:1, and the total number of viable bacteria of the compound probiotic is greater than 5x10 8 CFU / g.

[0073] S6: placing the fermentation solution in a natural fermentation container and fermenting at room temperature for at least 7 days, and then filtering to obtain a lotus root extract;

[0074] S7: storing the lotus root extract after sterilization for later use, or drying the lotus root extract at low temperature to make lotus root extract for storage.

[0075] It should be noted that the lotus root extract can be directly used in cosmetics after sterilization, and the sterilization conditions of the lotus root extract are low-temperature sterilization at 50°C for 30 minutes, and then high-temperature sterilization at 120°C for 20 seconds.

[0076] The starch content, transparency and swelling degree of lotus root powder and lotus root extract made by the method of embodiments 1-3 were determined.

[0077] In the present application, the determination of starch content is carried out according to the determination method of GB 5009.9-2016 "National Food Safety Standard Determination of Starch in Food". The specific method is acid hydrolysis method. After removing fat and soluble sugars from the sample, the starch therein is hydrolyzed into monosaccharides with reducing properties by acid, and then the reducing sugar is determined and converted into starch.

[0078] The determination method of transparency is as follows: 1 g of lotus root powder, 1 g of lotus root extract A prepared by the method of Example 1, 1 g of lotus root extract B prepared by the method of Example 2 and 1 g of lotus root extract C prepared by the method of Example 3 are taken, and pure water is added to prepare 1% lotus root powder solution, 1% lotus root extract solution A, 1% lotus root extract solution B and 1% lotus root extract solution C, respectively. After being placed in a 100℃ condition for 15 minutes, then cooled to 25 degrees, TU-1810S ultraviolet visible spectrophotometer is used to determine the transmittance of lotus root powder solution, lotus root extract solution A, lotus root extract solution B and lotus root extract solution C at wavelength 620nm with distilled water as blank.

[0079] The determination method of swelling degree is as follows: 0.5g of dry starch is mixed with 25ml of distilled water in a pre-weighed 50ml centrifuge tube, and then oscillated in a 95℃ water bath for 30 minutes. Then, the supernatant is discarded after centrifugation at 3000r / min for 15 minutes, and the mass of the precipitate after centrifugation is weighed. The swelling degree of the sample is calculated according to the following formula:

[0080] Swelling degree / % = mass of precipitate after centrifugation / sample mass*100

[0081] Statistical analysis: measurement data is expressed as mean +- standard deviation, and SPSS software is used for statistical analysis. If the variance of measurement data is homogeneous, single factor analysis of variance is used; the least significant difference method is used for comparison between groups; if the variance of measurement data is not homogeneous, single factor analysis of variance is used for test, P<0.05 is considered to have statistical significance.

[0082] The specific determination results are shown in Table 1:

[0083] Table 1 Physical and chemical index determination data of lotus root powder and lotus root extract

[0084] Test article Starch content / % Transparency / % Swelling degree / % Lotus root starch 32.15±0.65 21.81±0.14 823.64±31.50 Example 1 10.14±0.71* 32.41±1.13* 1110.12±64.29* Example 2 10.45±0.65* 31.82±1.08* 1113.06±65.18* Example 3 10.04±0.68* 32.55±1.25* 1109.65±63.73*

[0085] Example 1, Example 2 and Example 3 are compared with the comparative lotus root powder, and the difference is statistically significant.

[0086] As shown in Table 1, the starch content of lotus root powder extract is obviously lower than that of lotus root powder, and the transparency and swelling degree of lotus root powder extract are obviously higher than those of lotus root powder with the same mass, so lotus root powder extract is more suitable for being used as a cosmetic raw material to prepare cosmetics.

[0087] The moisture retention function of lotus root powder and lotus root extract prepared by the method of Examples 1-3 was determined by using lotus root powder as a comparative example, and the specific determination method is as follows:

[0088] 1 g of lotus root powder, 1 g of lotus root extract A prepared by the method of Example 1, 1 g of lotus root extract B prepared by the method of Example 2 and 1 g of lotus root extract C prepared by the method of Example 3 were respectively weighed and dispersed in 15 ml of distilled water, and then were respectively placed in a water bath at 45℃, 55℃, 65℃, 75℃ and 85℃ for heating for 30 min, and then were cooled to 25 degrees, and then were centrifuged at 3000 r / min for 15 min by using a centrifuge, and then the supernatant was discarded, and the mass of the precipitate was weighed. The moisture retention was calculated according to the following formula:

[0089] Moisture retention / % = mass of precipitate / sample mass*100

[0090] The specific determination results are shown in Table 2.

[0091] Table 2: Moisture retention determination data of lotus root powder and lotus root extract

[0092]

[0093] The lotus root molecules form hydrophilic colloids in water, and with the increase of temperature, the starch molecules are gradually gelatinized and broken to generate more hydrogen bonds. As shown in Table 2, the moisture retention of lotus root powder and lotus root extract is roughly the same in the temperature range of 45-75℃, but in the temperature range of 75-85℃ close to the gelatinization temperature, the moisture retention of lotus root extract is higher than that of lotus root powder, because a large amount of water is absorbed in the starch gelatinization process. Finally, under the condition of 85 degrees, the moisture retention of lotus root powder is about 1200%, and the moisture retention of lotus root extract is more than 1500%. Therefore, it can be seen that the moisture retention of lotus root extract is higher than that of lotus root powder.

[0094] Example 4

[0095] The moisturizing water provided by Example Four of the present application is mainly prepared by mixing the following components in parts by weight: 5 parts of lotus root extract, 5 parts of glycerol, 1 part of 1% hyaluronic acid, 1 part of tremella polysaccharide and the balance of ultrapure water.

[0096] The lotus root extract in the present example is prepared by the method of Example 1, Example 2 or Example 3.

[0097] Example 5

[0098] Example 5 provides a moisturizing water, which is mainly made by mixing the following components in parts by weight: lotus root extract 20 parts, glycerin 10 parts, 1% hyaluronic acid 5 parts, tremella polysaccharide 5 parts, and the balance of ultrapure water.

[0099] The lotus root extract in this example is the lotus root extract prepared by the method of Example 1, Example 2, or Example 3.

[0100] Example 6

[0101] Example 6 provides a moisturizing water, which is mainly made by mixing the following components in parts by weight: lotus root extract 1 part, glycerin 7 parts, 1% hyaluronic acid 2 parts, tremella polysaccharide 6 parts, and the balance of ultrapure water.

[0102] The lotus root extract in this example is the lotus root extract prepared by the method of Example 1, Example 2, or Example 3.

[0103] Experiment of moisturizing evaluation

[0104] According to the lotus root powder extract 1%, 5%, 10%, the balance is ultrapure water, and the moisturizing water of low, medium and high concentration groups is configured, which are marked as Comparative Example 1, Comparative Example 2 and Comparative Example 3, respectively, and then compared with the moisturizing water of Example 4, Example 5 and Example 6 and pure water.

[0105] Subject inclusion criteria: 20-55 years old, healthy, and qualified after inquiry, all of which can be used for observation. Subject exclusion criteria: those with severe systemic diseases, immune deficiency or autoimmune diseases; those with active allergic diseases; pregnant or lactating women; those who use hormone and immune agents during the test period; those who do not use the test material or have incomplete data. All subjects sign the informed consent form.

[0106] Before the test, the test site cannot use any product (cosmetic or external drug) for 2-3 days. During the experiment, the subjects clean the inner measurement of the forearm, mark the measurement area on the inner measurement of the forearm. Test environment conditions: the test environment temperature is 22±1℃, the humidity is 50±5%, no drinking water, the forearm is exposed, relaxed, and real-time dynamic detection is carried out;

[0107] The test area and the control area are measured by using the capacitance method skin tester. According to the experimental design, the TEWL value of each period is measured, and the skin water loss reduction calculation formula is as follows:

[0108] T% skin water loss reduction percentage = (skin water loss amount before use T0-T1 skin water loss amount after use) / skin water loss amount before use T0x 100%

[0109] Table 3 Amount of skin moisture loss (%)

[0110]

[0111]

[0112] Note: The negative value in the table represents the increase in the amount of skin moisture loss relative to T0; the positive value represents the decrease in the amount of skin moisture loss relative to T0.

[0113] As can be seen from Table 3, the low, medium and high concentration solutions prepared using the lotus root powder extract of the present application can effectively reduce the loss of skin moisture content, and can also play a moisturizing effect when used alone. The moisturizing effect of the moisturizing water prepared by adding the lotus root powder extract of the present application to other cosmetics with moisturizing effect, such as the moisturizing water of Example 4, Example 5 or Example 6 of the present application, is better.

[0114] The present application is not limited to the above-mentioned optional embodiments, and anyone can derive other various forms of products under the inspiration of the present application. The above-mentioned specific embodiments should not be understood as limiting the scope of protection of the present application, and the scope of protection of the present application should be defined by the claims, and the specification can be used to interpret the claims.

Claims

1. A method for preparing a lotus root extract, characterized in that: The following steps are involved: S1: Prepare lotus root starch from lotus root; S2: taking an appropriate amount of lotus root powder, adding purified water and stirring evenly to obtain lotus root pulp, and then sterilizing the lotus root pulp by pasteurization; S3: Using a pH regulator to adjust the pH of the sterilized lotus root pulp to a pH of 5.5 to 6.5; S4: adding cellulase, pectinase and amylase to the lotus root pulp, and then performing enzymatic hydrolysis at 45-60° C. for 24-48 hours, and filtering to obtain an enzymatic hydrolyzate; The weight ratio of lotus root pulp, cellulase, pectinase and amylase is: 1000 parts of lotus root pulp, 6-7 parts of cellulase, 6-12 parts of pectinase, and 3-5 parts of amylase; S5: sterilizing the enzymatic hydrolysate by pasteurization, allowing it to stand until it reaches room temperature, adding the compound probiotics, stirring evenly, and fermenting it at 36-38° C. for 12-72 hours, until the acidity reaches a pH of 4-5, to obtain a fermentation liquid; The weight ratio of the added amount of the composite probiotics to the enzymatic hydrolysate is 1 to 3:

200. The composite probiotics are mainly prepared by mixing Lactobacillus plantarum, Bacillus subtilis and yeast in a weight ratio of 1:1:

1. The total number of viable bacteria of the composite probiotics is greater than 5×10 8 CFU / g; S6: placing the fermentation liquid in a natural fermentation container, fermenting at room temperature for at least 7 days, and then filtering to obtain a lotus root extract; S7: sterilize the lotus root extract and store it for later use, or dry the lotus root extract at low temperature to prepare lotus root extract and store it for later use.

2. The method for preparing the lotus root extract according to claim 1, wherein: In step S1, the ground lotus root starch is sieved using a 100-mesh sieve.

3. The method for preparing the lotus root extract according to claim 1, wherein: In step S2, the volume ratio of the amount of pure water added to the amount of lotus root starch is 4 to 6:

1.

4. The method for preparing the lotus root extract according to claim 1, wherein: In step S3, acetic acid is used as the pH adjuster.

5. The method for preparing the lotus root extract according to claim 1, wherein: In step S5, the enzymatic hydrolysate was sterilized at 85°C for 15 minutes.

6. The method for preparing the lotus root extract according to claim 1, wherein: In step S7, the lotus root extract is sterilized by low-temperature sterilization at 40-60° C. for 20-35 minutes and then high-temperature sterilization at 105-135° C. for 15-30 seconds.

7. Use of the lotus root extract prepared according to the preparation method according to any one of claims 1 to 6 in the preparation of cosmetics.

8. A moisturizing lotion, characterized by: The invention is mainly prepared by mixing the following components in parts by weight: 1 to 20 parts of lotus root extract prepared by the preparation method according to any one of claims 1 to 6, 1 to 10 parts of glycerin, 1 to 10 parts of 1% hyaluronic acid, 1 to 10 parts of tremella polysaccharide and the balance of ultrapure water.

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