A separation device of natto kinase and a refining method thereof

Abstract

The application relates to the technical field of separation equipment, and discloses a separation device for natto kinase and a refining method thereof, which comprises a separation tank, the top end of the separation tank is provided with a separation cover, the middle part of the separation cover is provided with a second motor box, one side of the middle part of the separation cover is provided with a feeding hopper, the second motor box of the separation cover is provided with a first motor box, the bottom end of the first motor box is provided with a rotating rod body, the bottom end of the rotating rod body is fixedly connected with a disc incense-shaped stirring blade, the top end of the disc incense-shaped stirring blade is fixedly connected with equidistantly-arranged supporting rods, the low-stress rope is arranged, when the low-stress rope touches the natto sticking together, the low-stress rope plays a role similar to that of a wire cutting machine, can cut the natto gathered on the inner wall of the separation tank in the rotating process, and since the physiological saline water dilution leads to the decrease of the viscosity of the natto sticking together, the natto can be easily separated when being stirred.

Description

Technical Field

[0001] This invention relates to the field of separation equipment technology, and more specifically to a separation device for nattokinase and its extraction method. Background Technology

[0002] Nattokinase, also known as Bacillus subtilis protease, is a serine protease produced by Bacillus subtilis during natto fermentation. It has effects such as dissolving blood clots, reducing blood viscosity, improving blood circulation, softening blood vessels, and increasing vascular elasticity.

[0003] Because natto has a distinctive odor that is difficult for most people to tolerate, a solution is to extract nattokinase from natto. This allows nattokinase to be used without being bothered by the odor. To achieve this, nattokinase is extracted by stirring and separating the natto, and then purified. The extracted nattokinase is then used to produce the corresponding product.

[0004] The existing technology for separating nattokinase mainly involves culturing Bacillus subtilis produced during the fermentation of soybeans into natto to generate nattokinase. However, in actual use, the traditional separation method is slow and the separation process is cumbersome. Therefore, this invention provides a separation device and its extraction method. Summary of the Invention

[0005] In order to overcome the above-mentioned defects of the prior art, the present invention provides a nattokinase separation device and its extraction method to solve the problems existing in the background art.

[0006] This invention provides the following technical solution: a separation device and extraction method for nattokinase, comprising a separation tank, a separation cover at the top of the separation tank, a second motor housing installed in the middle of the separation cover, a feeding funnel installed on one side of the middle of the separation cover, a first motor housing installed in the second motor housing of the separation cover, a rotating rod body installed at the bottom of the first motor housing, a coil-shaped stirring blade fixedly connected to the bottom of the rotating rod body, and equidistantly arranged support rods fixedly connected to the top of the coil-shaped stirring blade on the rotating rod body, each support rod having a limit rod fixedly connected to its bottom. The limit rod, a low-stress rope, and the rotating rod... Each moving rod body has a through groove. The limiting rod, the low-stress rope, and the rotating rod body all have low-stress ropes in their through grooves. The low-stress ropes at the bottom of the limiting rod are connected to the top of the largest ring of the coil-shaped stirring blade. The support rod has a steering wheel installed at the through groove of the limiting rod. The rotating rod body also has a steering wheel installed at the end near the support rod. The top of the rotating rod body has a winding groove. The side wall of the winding groove at the low-stress rope is equipped with a U-shaped block. The limiting block has a winding rod in the middle. The winding rod passes through the rotating rod body and is fixedly connected to a winding wheel in the winding groove.

[0007] Furthermore, the low-stress rope at one end of the winding groove passes through the U-shaped hole of the U-shaped block and is fixedly connected to the winding wheel. A telescopic groove is provided in the middle of the bottom end of the first motor box. The top end of the rotating rod body is located in the telescopic groove and is movably engaged. Telescopic rods are installed on both sides of the winding rod in the telescopic groove. The top ends of the telescopic rods are fixedly connected to the top end of the inner wall of the telescopic groove of the first motor box, and the bottom ends of the telescopic rods are fixedly connected to the top end of the rotating rod body.

[0008] Furthermore, the first motor housing is equipped with a first motor, and the output shaft of the first motor in the first motor housing is fixedly connected to the winding rod. The second motor housing is equipped with a second motor, and the second motor in the second motor housing controls the first motor housing to rotate. The bottom of the telescopic groove of the first motor housing is provided with equidistantly arranged limiting grooves, and the outer wall of the rotating rod body is provided with limiting blocks that are movably connected to the limiting grooves.

[0009] Furthermore, the outer wall of the separation tank is provided with equidistant observation windows, the observation windows are made of transparent tempered glass, and the separation tank is provided with scales on both sides of the observation windows. The scales of the observation windows are used to observe the height of the supernatant. The inner wall of the separation tank is inclined, and a turbidity drain valve is installed at the lowest point of the separation tank that is inclined with the inner wall. A supernatant drain valve is installed on the outer wall of the separation tank.

[0010] Furthermore, each of the incense-shaped stirring blades has a first inverted L-shaped groove on the side of its cross-section closest to the inner wall of the separation tank. Each of the first inverted L-shaped grooves has a retaining groove at its bottom end. Each of the incense-shaped stirring blades has a second inverted L-shaped groove symmetrical to the first inverted L-shaped groove on the side of its cross-section closest to the rotating rod body. Each of the second inverted L-shaped grooves has a retaining post that cooperates with the retaining groove of the first inverted L-shaped groove. The incense-shaped stirring blades are made of stainless steel. Each of the outermost rings of the incense-shaped stirring blades has a protective pad. The incense-shaped stirring blades can be combined into a single disc via a low-stress rope and the rotating rod body.

[0011] Furthermore, the second motor housing is equipped with a telescopic control unit for the telescopic rod, and the telescopic length of the second motor housing is controlled by a computer.

[0012] A method for extracting nattokinase, comprising the following:

[0013] S1. First, select soybeans. When selecting soybeans, ensure that some black soybeans are mixed with yellow soybeans. Wash the mixed soybeans thoroughly. After washing, dry the mixed soybeans. Soak the soybeans after washing. After soaking, use high-pressure steaming. During high-pressure steaming, ensure that the mixed soybeans are cooked. Stir constantly during steaming. Continue stirring until the temperature reaches 100℃. Maintain 100℃ for 10 minutes, then continue to raise the temperature until it reaches 120℃ and maintain 120℃ for 10 minutes. This ensures that the mixed soybeans are cooked.

[0014] S2. Cool the cooked mixed beans. While cooling, you can choose whether to inoculate with Bacillus subtilis and then ferment. During fermentation, you need to control the temperature to ensure that the temperature is at the optimal temperature of Bacillus subtilis, 37°C. After 24 hours, perform a low-temperature post-ripening operation to obtain mature natto.

[0015] S3. By placing the natto in low-temperature physiological saline, the nattokinase separation device is started and stirred. As stirring continues, the white film and strings of the natto no longer adhere to the surface of the natto but disperse in the physiological saline. At this point, by raising the entire rotating rod, the mixture of nattokinase and physiological saline can be obtained for subsequent purification.

[0016] The technical effects and advantages of this invention are as follows:

[0017] This invention features a coil-shaped stirring blade. As the coil-shaped stirring blade passes through the slot of the first inverted L-shaped groove and contacts the pin of the second inverted L-shaped groove, a flat surface is formed. At this point, the turbidity drain valve is opened, and the rotating rod body is pushed by the telescopic rod, causing the disc formed by the coil-shaped stirring blade to be squeezed, thereby squeezing out the natto precipitate through the turbidity drain valve. Then, the entire rotating rod body is lifted by the telescopic rod. As the rotating rod body is lifted, the supernatant drain valve is opened, allowing the supernatant to flow out and be collected. This completes the collection of the mixture of nattokinase and physiological saline from natto.

[0018] This invention incorporates a low-stress rope. When the low-stress rope comes into contact with natto that is stuck together, it acts like a wire cutter, cutting the natto that has gathered on the inner wall of the separation tank during rotation. Because the saline solution dilutes the sticky natto, the sticky natto becomes less sticky, making it easy to separate the sticky natto during stirring.

[0019] This invention features a coil-shaped stirring blade. Due to the special shape of the coil-shaped stirring blade, the stirred liquid will separate into natto in the direction of rotation. When rotating in the forward direction, the mixed liquid will impact the bottom of the inner wall of the separation tank, while when rotating in the reverse direction, the mixed liquid will surge towards the top of the inner wall of the separation tank. This increases the flow of the mixed liquid and allows for more thorough mixing. Attached Figure Description

[0020] Figure 1 This is a schematic diagram of the overall structure of the present invention;

[0021] Figure 2 This is an exploded view of the overall structure of the present invention;

[0022] Figure 3 This is a schematic diagram of the main structure of the rotating rod of the present invention;

[0023] Figure 4 This is a schematic diagram of the internal components of the rotating rod body of the present invention;

[0024] Figure 5 This is a schematic cross-sectional view of the main body of the rotating rod of the present invention;

[0025] Figure 6 This is a schematic diagram of the winding groove structure of the present invention;

[0026] Figure 7 This is a schematic cross-sectional view of the expansion slot of the first motor housing of the present invention;

[0027] Figure 8 This is a schematic cross-sectional view of the support rod of the present invention;

[0028] Figure 9 This is a schematic diagram of the cross-sectional structure of the incense-shaped stirring blade of the present invention;

[0029] Figure 10 For the present invention Figure 9 Enlarged schematic diagram of the structure at point A.

[0030] The attached diagram is labeled as follows: 1. Separation tank; 101. Separation cover; 102. Feeding funnel; 103. Observation window; 104. Turbid discharge valve; 105. Supernatant discharge valve; 2. Second motor box; 201. First motor box; 202. Telescopic groove; 203. Telescopic rod; 204. Limiting groove; 3. Rotating rod body; 301. Limiting block; 3011. Winding rod; 3012. Winding groove; 302. Support rod; 303. Limiting rod; 304. Low-stress rope; 3041. U-shaped block; 305. Steering wheel; 306. Winding wheel; 4. Incense-shaped stirring blade; 401. First inverted L-shaped groove; 402. Second inverted L-shaped groove. Implementation

[0031] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings. In addition, the morphology of each structure described in the following embodiments is merely illustrative. The purification and extraction method and extraction apparatus of nattokinase involved in the present invention are not limited to the structures described in the following embodiments. All other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0032] Reference Figure 1-10This invention provides a device for separating nattokinase and a method for refining it, comprising a separation tank 1, a separation cover 101 at the top of the separation tank 1, a second motor housing 2 installed in the middle of the separation cover 101, a feeding funnel 102 installed on one side of the middle of the separation cover 101, a first motor housing 201 installed in the second motor housing 2 of the separation cover 101, a rotating rod body 3 installed at the bottom of the first motor housing 201, a coil-shaped stirring blade 4 fixedly connected to the bottom of the rotating rod body 3, and equidistantly arranged support rods 302 fixedly connected to the top of the rotating rod body 3 at the top of the coil-shaped stirring blade 4. Each support rod 302 has a limiting rod 303 fixedly connected to its bottom. The limiting rod 303, the low-stress rope 304, and the rotating rod body 3 all have passage grooves. Low-stress ropes 304 are installed in the grooves of the limiting rod 303, the low-stress rope 304, and the rotating rod body 3. The low-stress ropes 304 at the bottom of the limiting rod 303 are connected to the top of the largest ring of the coil-shaped stirring blade 4. The support rod 302 is equipped with a steering wheel 305 at the groove of the limiting rod 303. During the later stirring process, the low-stress ropes 304 will undergo a slight deformation, which will cause the low-stress ropes 304 at the bottom of the coil-shaped stirring blade 4 and the limiting rod 303 to be closer to the inner wall of the separation tank 1. This will allow the natto pieces that have diffused on the inner wall of the separation tank 1 during stirring to be cut apart in a manner similar to wire cutting, thus accelerating the separation of the sticky natto pieces. The rotating rod body 3, located near the support rod 302, is equipped with a steering wheel 305. A winding groove 3012 is formed inside the top of the rotating rod body 3. U-shaped blocks 3041 are installed on the side walls of the winding groove 3012 at the low-stress rope 304. A winding rod 3011 is located in the middle of the limiting block 301. The winding rod 3011 passes through the rotating rod body 3 and is fixedly connected to a winding wheel 306 within the winding groove 3012. The low-stress rope 304 at one end of the winding groove 3012 passes through the U-shaped hole of the U-shaped block 3041 and is fixedly connected to the winding wheel 306. A telescopic groove 202 is formed in the middle of the bottom of the first motor housing 201. The top of the rotating rod body 3 is located in the telescopic groove 202 and is engaged in a movable manner. Telescopic rods 203 are installed on both sides of the winding rod 3011. The top of each telescopic rod 203 is fixedly connected to the top of the inner wall of the telescopic groove 202 of the first motor box 201, and the bottom of each telescopic rod 203 is fixedly connected to the top of the rotating rod body 3. The first motor box 201 is equipped with a first motor, and the output shaft of the first motor in the first motor box 201 is fixedly connected to the winding rod 3011. The second motor box 2 is equipped with a second motor, and the second motor in the second motor box 2 controls the first motor box 201 to rotate. The bottom of the telescopic groove 202 in the first motor box 201 is provided with equidistantly arranged limiting grooves 204. The outer wall of the rotating rod body 3 is provided with limiting blocks 301 that are movably connected to the limiting grooves 204.

[0033] The outer wall of the separation tank 1 is provided with observation windows 103 arranged at equal intervals. The observation windows 103 are made of transparent tempered glass. The separation tank 1 is provided with scales on both sides of the observation windows 103. The scales of the observation windows 103 are used to observe the height of the supernatant. The inner wall of the separation tank 1 is inclined. The turbidity drain valve 104 is installed at the lowest point of the inclination with the inner wall of the separation tank 1. The outer wall of the separation tank 1 is provided with a supernatant drain valve 105.

[0034] The incense-shaped stirring blade 4 has a first inverted L-shaped groove 401 on the side of its cross-section closest to the inner wall of the separation tank 1. The bottom of the first inverted L-shaped groove 401 has a slot. The incense-shaped stirring blade 4 has a second inverted L-shaped groove 402 symmetrical to the first inverted L-shaped groove 401 on the side of its cross-section closest to the rotating rod body 3. The second inverted L-shaped groove 402 has a locking post that cooperates with the slot of the first inverted L-shaped groove 401. The incense-shaped stirring blade 4 is made of stainless steel. The outer wall of the largest ring of the incense-shaped stirring blade 4 is provided with a protective pad. The incense-shaped stirring blade 4 can be combined into a disc by the low-stress rope 304 and the rotating rod body 3. The shape of the incense-shaped stirring blade 4 is like an incense coil. Under normal use, due to gravity, its shape on the side is similar to a frustum, thus forming a structure similar to the piston of a syringe for separation.

[0035] The second motor housing 2 is equipped with a telescopic control unit for the telescopic rod 203, and the telescopic length of the second motor housing 2 is controlled by a computer.

[0036] A method for extracting nattokinase, comprising the following:

[0037] S1. First, select soybeans. When selecting soybeans, ensure that some black soybeans are mixed with yellow soybeans. Wash the mixed soybeans thoroughly. After washing, dry the mixed soybeans. Soak the soybeans after washing. After soaking, use high-pressure steaming. During high-pressure steaming, ensure that the mixed soybeans are cooked. Stir constantly during steaming. Continue stirring until the temperature reaches 100℃. Maintain 100℃ for 10 minutes, then continue to raise the temperature until it reaches 120℃ and maintain 120℃ for 10 minutes. This ensures that the mixed soybeans are cooked.

[0038] S2. Cool the cooked mixed beans. While cooling, you can choose whether to inoculate with Bacillus subtilis and then ferment. During fermentation, you need to control the temperature to ensure that the temperature is at the optimal temperature of Bacillus subtilis, 37°C. After 24 hours, perform a low-temperature post-ripening operation to obtain mature natto.

[0039] S3. By placing natto in low-temperature physiological saline, the nattokinase separation device is activated and stirred. As stirring continues, the white film and stringy texture of the natto no longer adhere to the surface but disperse in the physiological saline. At this point, by raising the rotating rod body 3, the mixture of nattokinase and physiological saline is obtained, thus facilitating subsequent purification.

[0040] The working principle of this invention is as follows: After the natto fermentation is complete, the fermented natto is fed into the feeding funnel 102. As it falls into the feeding funnel 102, the natto enters the separation tank 1. Physiological saline solution is then added. After adding the saline solution, the second motor in the second motor housing 2 is started. Due to the constraints of the limiting block 301 and the limiting groove 204, the second motor drives the rotating rod body 3 to rotate. As the rotating rod body 3 rotates, it causes the support rod 302, the limiting rod 303, the low-stress rope 304, and the coil-shaped stirring blade 4 to rotate. This rotation causes the mixture of natto and physiological saline solution to form a liquid. During the stirring process, the limiting rod 303 and the support rod 302 also stir the mixture. The low-stress rope 304 also stirs the mixture. When the low-stress rope 304 touches the sticky natto, the dilution effect of the saline solution reduces the stickiness of the natto, making it easy to separate the sticky natto. Simultaneously, due to the special shape of the incense-shaped stirring blade 4, the liquid being stirred separates the natto in the direction of rotation. When rotating forward, the mixed liquid impacts the bottom of the inner wall of the separation tank 1, while when rotating in reverse, it surges towards the top of the inner wall of the separation tank 1, thereby increasing the flow of the mixed liquid. This allows for more thorough mixing of the liquid. After stirring stops, the mixture begins to separate into layers. The position of the supernatant can be determined through the observation window 103. The telescopic rod 203 is then extended or retracted to ensure the top of the rotating rod body 3 is positioned at the separation point between the supernatant and the lower turbidity. Simultaneously, the first motor is started to rotate. As the first motor rotates, the winding rod 3011 rotates, causing the winding wheel 306 to rotate. With the rotation of the winding wheel 306, the low-stress rope 304 is transported from the U-shaped block 3041 to the winding wheel 306. As the winding wheel 306 rotates, the transported low-stress rope 304 winds onto the winding wheel 306, thus causing the low-stress rope to... The force rope 304 is wound by the winding wheel 306, which causes the steering wheel 305 at the rotating rod body 3 and the support rod 302 to pull the low-stress rope 304 at the limiting rod 303, thereby pulling the maximum circle of the coil-shaped stirring blade 4. Due to the special design of the cross-section of the coil-shaped stirring blade 4, the coil-shaped stirring blade 4 is pulled. As the coil-shaped stirring blade 4 contacts the locking post of the second inverted L-shaped groove 402 through the locking groove of the first inverted L-shaped groove 401, a plane is formed. At this time, by opening the turbid liquid drain valve 104, the rotating rod body 3 is pushed by the telescopic rod 203, thereby squeezing the disc formed by the coil-shaped stirring blade 4, and thus squeezing out the natto sediment through the turbid liquid drain valve 104.Then, the entire rotating rod body 3 is lifted using the telescopic rod 203. As the rotating rod body 3 is lifted, the supernatant drain valve 105 is opened, allowing the supernatant to flow out and be collected. This completes the collection of the mixture of nattokinase and physiological saline from natto.

[0041] In conclusion, the above are merely preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A device for separating nattokinase, comprising a separation tank (1), characterized in that: The top of the separation tank (1) is provided with a separation cover (101). A second motor box (2) is installed in the middle of the separation cover (101). A feeding funnel (102) is installed on one side of the middle of the separation cover (101). A first motor box (201) is installed in the second motor box (2) of the separation cover (101). A rotating rod body (3) is installed at the bottom of the first motor box (201). A coil-shaped stirring blade (4) is fixedly connected to the bottom of the rotating rod body (3). Equally spaced support rods (302) are fixedly connected to the top of the coil-shaped stirring blade (4). A limit rod (303) is fixedly connected to the bottom of each support rod (302). A through groove is opened in the limit rod (303), the low-stress rope (304) and the rotating rod body (3). Low-stress ropes (304) are provided in the through grooves of the rotating rod body (3) and the bottom of the limiting rod (303) are connected to the top of the largest circle of the incense-shaped stirring blade (4). The support rod (302) is equipped with a steering wheel (305) at the through groove of the limiting rod (303). The rotating rod body (3) is also equipped with a steering wheel (305) at the end near the support rod (302). The top of the rotating rod body (3) is provided with a winding groove (3012). The side wall of the winding groove (3012) is equipped with a U-shaped block (3041) at the low-stress rope (304). The middle of the limiting block (301) is provided with a winding rod (3011). The winding rod (3011) passes through the rotating rod body (3) and is fixedly connected to a winding wheel (306) in the winding groove (3012).

2. The nattokinase separation device according to claim 1, characterized in that: The low-stress rope (304) passes through the U-shaped hole of the U-shaped block (3041) at one end of the winding groove (3012) and is fixedly connected to the winding wheel (306). The bottom center of the first motor box (201) is provided with a telescopic groove (202). The top of the rotating rod body (3) is located in the telescopic groove (202) and is movably engaged. Telescopic rods (203) are installed on both sides of the winding rod (3011) in the telescopic groove (202). The top of the telescopic rods (203) is fixedly connected to the top of the inner wall of the telescopic groove (202) of the first motor box (201). The bottom of the telescopic rods (203) is fixedly connected to the top of the rotating rod body (3).

3. The nattokinase separation device according to claim 1, characterized in that: The first motor box (201) is equipped with a first motor inside. The output shaft of the first motor in the first motor box (201) is fixedly connected to the winding rod (3011). The second motor box (2) is equipped with a second motor inside. The second motor in the second motor box (2) controls the first motor box (201) to rotate. The bottom of the telescopic groove (202) of the first motor box (201) is provided with equidistantly arranged limiting grooves (204). The outer wall of the rotating rod body (3) is provided with limiting blocks (301) that are movably connected to the limiting grooves (204).

4. The nattokinase separation device according to claim 1, characterized in that: The outer wall of the separation tank (1) is provided with observation windows (103) arranged at equal intervals. The observation windows (103) are made of transparent reinforced glass. The separation tank (1) is provided with scales on both sides of the observation windows (103). The scales of the observation windows (103) are used to observe the height of the supernatant. The inner wall of the separation tank (1) is inclined. The separation tank (1) is equipped with a turbidity drain valve (104) at the lowest point of the inclination with the inner wall. The outer wall of the separation tank (1) is equipped with a supernatant drain valve (105).

5. The nattokinase separation device according to claim 1, characterized in that: The incense-shaped stirring blade (4) has a first inverted L-shaped groove (401) on the side of its cross-section near the inner wall of the separation tank (1). The bottom of the first inverted L-shaped groove (401) has a slot. The incense-shaped stirring blade (4) has a second inverted L-shaped groove (402) symmetrical to the first inverted L-shaped groove (401) on the side of its model near the rotating rod body (3). The second inverted L-shaped groove (402) has a locking post that cooperates with the slot of the first inverted L-shaped groove (401). The incense-shaped stirring blade (4) is made of stainless steel. The outer wall of the largest circle of the incense-shaped stirring blade (4) is provided with a protective pad. The incense-shaped stirring blade (4) can be combined into a disc by means of a low-stress rope (304) and the rotating rod body (3).

6. The nattokinase separation device according to claim 1, characterized in that: The second motor housing (2) is equipped with a telescopic control unit for the telescopic rod (203), and the telescopic length of the second motor housing (2) is controlled by a computer.

7. A method for extracting nattokinase, using a nattokinase separation apparatus as described in any one of claims 1-6, characterized in that: Includes the following: S1. First, select soybeans. When selecting soybeans, ensure that some black soybeans are mixed with yellow soybeans. Wash the mixed soybeans thoroughly. After washing, dry the mixed soybeans. Soak the soybeans after washing. After soaking, use high-pressure steaming. During high-pressure steaming, ensure that the mixed soybeans are cooked. Stir constantly during steaming. Continue stirring until the temperature reaches 100℃. Maintain 100℃ for 10 minutes, then continue to raise the temperature until it reaches 120℃ and maintain 120℃ for 10 minutes. This ensures that the mixed soybeans are cooked. S2. Cool the cooked mixed beans. While cooling, you can choose whether to inoculate with Bacillus subtilis and then ferment. During fermentation, you need to control the temperature to ensure that the temperature is at the optimal temperature of Bacillus subtilis, 37°C. After 24 hours, perform a low-temperature post-ripening operation to obtain mature natto. S3. By placing the natto into low-temperature saline solution, the nattokinase separation device is started and stirred. As the stirring continues, the white film and filaments of the natto no longer adhere to the surface of the natto, but disperse in the saline solution. At this time, by raising the entire rotating rod body (3), the mixture of nattokinase and saline solution can be purified in the later stage.

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