A large-scale and easy-to-operate haploid chemical doubling device

Abstract

The present invention relates to the technical field of corn breeding, and discloses a large-scale easy-to-operate haploid chemical doubling device, including a seedling basket for holding seedlings; a medicine basin for storing liquid medicine and for placing a seedling basket bottom basin, which is used to place the medicine basin and the seedling basket, and a top cover for sealing the opening of the bottom basin. The seedling basket, medicine basin, bottom basin and top cover in the present invention are all made of plastic products. The specification of the bottom basin is 60*40*19cm. This scheme adopts the root immersion method to soak the 2-3 leaf stage seedlings in a 4-5 cm deep colchicine solution, and the colchicine solution is stored in the medicine basin 2, and the bottom basin plays the role of sealing and isolation. A large number of seedlings can be processed at one time, which greatly improves work efficiency.

Description

Technical Field

[0001] The invention relates to the technical field of corn breeding, and in particular to a large-scale and easy-to-operate haploid chemical doubling device. Background Art

[0002] The doubled haploid (DH) technique is a simple and quick method for breeding maize inbred lines. Its basic process is to induce the lines to obtain haploid grains or plants in vivo, and then obtain homozygous diploid ears through artificial or natural doubling, from which superior lines can be selected.

[0003] There are two ways to double the haploid of corn: natural doubling and chemical artificial doubling. The frequency of natural doubling is often relatively low, and the natural doubling rate of most materials is less than 3%. Chemical doubling can reach more than 50%. The main methods used are seed soaking, bud soaking, root soaking, injection and embryo rescue chemical doubling. The germination rate of the seed soaking method is low, the bud soaking method has complicated steps and low survival rate, and the injection and embryo rescue require high skills of workers. The root soaking method can be operated in a short time and on a large scale. The root soaking method soaks the 2-3 leaf seedlings in a 4-5 cm deep colchicine solution. The price of colchicine is relatively high, 200-680 yuan / gram. When there are more materials, a large amount of use is required, and it has certain toxicity and cannot be kept in contact for a long time.

[0004] The current method is to directly put the seedlings in the liquid medicine, cover the lid, and take out the seedlings after treatment. A 40.5*32cm medicine box can hold up to 350 seedlings. It takes at least 15 minutes to put in and take out. It requires 3.5 grams of colchicine. Planting 8,000 seedlings per mu requires 8000 / 350*3.5=80 grams of colchicine. The cheapest price of colchicine is 220 yuan, a total of 220*80=17600, 2.2 yuan per seedling, and it may be higher as the price of colchicine rises.

[0005] Therefore, it is urgent to propose a relatively independent haploid processing device that can place a large number of seedlings. Summary of the invention

[0006] In order to solve the technical problems raised in the background technology, the present invention provides a large-scale and easy-to-operate haploid chemical doubling device.

[0007] The present invention is implemented by the following technical scheme: a large-scale and easy-to-operate haploid chemical doubling device, comprising:

[0008] A seedling basket, which is used to hold seedlings;

[0009] Medicine basin, which is used to store medicine solution and place seedling basket

[0010] The bottom basin is used to place the medicine pot and the seedling basket, and

[0011] A top cover is used to seal the opening of the bottom basin.

[0012] As a further improvement of the above solution, a plurality of elastic support mechanisms are distributed circumferentially on the inner wall of the medicine holding basin, and the elastic support mechanisms are pressed downward when the top rod cooperates with the bottom basin.

[0013] The elastic supporting mechanism can press the seedling basket into the medicine basin and make the medicine liquid cover the roots of the seedlings to a certain height.

[0014] As a further improvement of the above solution, a plurality of groups of clamping units clamped with the top of the bottom basin are arranged around the top cover, and a flange is arranged outward at the top opening of the bottom basin, and the clamping units are clamped with the bottom of the flange.

[0015] The above structure can facilitate the installation and disassembly of the top cover.

[0016] As a further improvement of the above scheme, the snap-in unit includes a movable hook and a connecting pin, wherein one end of the movable hook is rotatably connected to the top cover through the connecting pin, a return spring is installed on the connecting pin, and the other end of the movable hook is provided with a hook head that is snap-connected to the bottom of the flange.

[0017] When in use, the top cover is buckled onto the top opening of the bottom basin, and then the movable hook is toggled so that the hook head and the flange are engaged, and the installation is completed.

[0018] As a further improvement of the above scheme, the elastic support mechanism includes a support plate and two clamping blocks, wherein the two clamping blocks are respectively fixed on the inner wall of the medicine basin, and through holes are opened in the two clamping blocks, and a rotating pin is installed at the through hole. One end of the rotating pin extends out of the through hole and is installed with a torsion spring. A docking unit cooperating with the rotating pin is also installed in the through hole. One side of the support plate is located between the two clamping blocks and is detachably connected to the docking units on the two clamping blocks.

[0019] The elastic support mechanism can effectively support the seedling pot, and when the top cover is taken away, the seedling pot can be automatically lifted up, so that the liquid medicine entering the seedling pot and attached to the roots of the seedlings can be drained.

[0020] As a further improvement of the above scheme, the docking unit includes a docking joint and an intermediate docking column, wherein the intermediate docking column is movably arranged in the through hole, the docking joint is coaxially fixed at one end of the docking column, and the docking joint and the inner end of the rotating pin shaft are movably engaged to transmit torque, a docking slot is provided on the other side of the intermediate docking column, an installation slot is provided on the side of the support plate, a connecting shaft is movably provided in the installation slot, and the other end of the connecting shaft is used to engage with the docking slot to transmit torque, and the cross-section of the installation slot and the cross-section of the connecting shaft are regular polygons, so that the two can transmit torque when they cooperate.

[0021] The above structure can facilitate installation and disassembly between the support plate and the two clamping blocks.

[0022] As a further improvement of the above solution, a plurality of limiting grooves are provided on the inner wall of the through hole along the circumferential direction, and a plurality of elastic pins slidably matched with the limiting grooves are installed on the side wall of the rotating pin shaft.

[0023] The above structure can facilitate the installation and disassembly of the rotating pin.

[0024] As a further improvement of the above solution, a rounded corner is arranged at one end of the limiting groove close to the middle docking column, and a conical slope is arranged at a side of the middle docking column away from the connecting pin.

[0025] It is convenient to guide the elastic pin to retract inwards and facilitate the disassembly of the connecting pin shaft.

[0026] As a further improvement of the above solution, the rotating pin and the middle docking column are magnetically mutually repelled, and the inner end of the rotating pin is provided with a slot for engaging with the docking head.

[0027] In this way, the middle docking column can be stably connected to the connecting shaft, so that the support plate drives the connecting pin shaft to rotate in the through hole.

[0028] As a further improvement of the above solution, the clamp block has a protrusion on one side close to the support plate, and a limit plate is provided on the part of the connecting shaft outside the installation slot, and the support plate is made close to the clamp block, and the protrusion abuts against the limit plate to separate the connecting shaft and the docking slot.

[0029] Compared with the prior art, the present invention has the following beneficial effects:

[0030] The invention provides a large-scale and easy-to-operate haploid chemical doubling device, which comprises a seedling basket, a medicine basin 2, a bottom basin and a top cover, has a simple structure, is easy to carry and is quick to operate.

[0031] The seedling basket, medicine basin, bottom basin and top cover in the present invention are all made of plastic products. The specification of the bottom basin is 60*40*19cm. The present solution adopts the root immersion method to immerse the 2-3 leaf seedlings in a colchicine solution with a depth of 4-5 cm. The colchicine solution is stored in the medicine basin 2, and the bottom basin plays a role of sealing and isolation. A large number of seedlings can be processed at one time, which greatly improves the work efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1 An exploded perspective view of the haploid processing device proposed by the present invention;

[0033] Figure 2 It is a front view of the overall structure of the haploid processing device proposed by the present invention;

[0034] Figure 3This is a schematic diagram of the internal structure of the haploid processing device proposed by the present invention when in use;

[0035] Figure 4 The haploid processing device proposed by the present invention Figure 3 A magnified image of point A;

[0036] Figure 5 A schematic diagram of the structure of the elastic support mechanism of the haploid processing device proposed by the present invention;

[0037] Figure 6 The haploid processing device proposed by the present invention Figure 5 A magnified view of point B;

[0038] Figure 7 This is a structural schematic diagram of the haploid processing device proposed in Example 1 of the present invention when the support plate bounces up the seedling basket;

[0039] Figure 8 A schematic diagram of the structure of the haploid processing device proposed in Example 2 of the present invention when the support plate bounces up the seedling basket

[0040] Description of main symbols:

[0041] In the figure: bottom basin 1, medicine basin 2, seedling basket 3, top cover 4, support plate 5, mounting groove 6, limit plate 7, connecting shaft 8, fillet 9, limit groove 10, elastic pin 11, clamping block 12, rotating pin 13, torsion spring 14, docking joint 15, middle docking column 16, slope 17, movable hook 18, connecting pin 19, flange 20, hook 21. DETAILED DESCRIPTION

[0042] The present invention is further described below in conjunction with the accompanying drawings and specific implementation methods. It should be noted that, under the premise of no conflict, the various embodiments or technical features described below can be arbitrarily combined to form a new embodiment.

[0043] Embodiment 1:

[0044] Reference Figure 1-6 , a large-scale and easy-to-operate haploid chemical doubling device, comprising

[0045] The seedling basket 3 is used for containing seedlings; its specification is 40.5*32cm, and can hold up to 1200 seedlings.

[0046] The medicine holding basin 2 is used for storing the medicine liquid and for placing the seedling basket 3; the medicine liquid is colchicine.

[0047] The bottom basin 1 is used to place the medicine basin 2 and the seedling basket 3, and

[0048] The top cover 4 is used to seal the opening of the bottom basin 1 .

[0049] The seedling basket 3, the medicine basin 2, the bottom basin 1 and the top cover 4 in this scheme are all made of plastic. The bottom basin 1 has a specification of 60*40*19 cm. The device is used to soak the 2-3 leaf seedlings in a 4-5 cm deep colchicine solution by the root immersion method. The colchicine solution is stored in the medicine basin 2, and the bottom basin plays a role of sealing and isolation.

[0050] The inner wall of the medicine basin 2 is circumferentially distributed with a plurality of elastic support mechanisms, which are pressed down when the top rod 4 cooperates with the bottom basin 1. The elastic support mechanism can press the seedling basket 3 into the medicine basin 2 and make the medicine liquid submerge the roots of the seedlings to a certain height. The height is preferably 4-5 cm.

[0051] Several groups of clamping units are arranged around the top cover 4 and are clamped with the top of the bottom basin 1, and a flange 20 is arranged outside the top opening of the bottom basin 1, and the clamping units are clamped with the bottom of the flange 20. Through the above structure, the top cover can be easily installed and removed.

[0052] The snap-on unit includes a movable hook 18 and a connecting pin 19, wherein one end of the movable hook 18 is rotatably connected to the top cover 4 via the connecting pin 19, and a return spring (not shown) is installed on the connecting pin, and the elastic force of the return spring can make the movable hook 18 approach the side wall of the bottom basin 1, and the other end of the movable hook 18 is provided with a hook head 21 snapped with the bottom of the flange 20. When in use, the top cover 4 is snapped on the top opening of the bottom basin 1, and then the movable hook 9 is toggled so that the hook head 21 is snapped with the flange 20, and the installation is completed.

[0053] The elastic support mechanism includes a support plate 5 and two clamping blocks 12, wherein the two clamping blocks 12 are respectively fixed to the inner wall of the medicine basin 2, and through holes are opened in the two clamping blocks 12, and a rotating pin 13 is installed at the through hole, one end of the rotating pin 13 extends out of the through hole, and a torsion spring 14 is installed, and a docking unit cooperating with the rotating pin 13 is also installed in the through hole, and one side of the support plate 5 is located between the two clamping blocks 12 and is detachably connected to the docking units on the two clamping blocks 12. The above-mentioned elastic support mechanism can effectively support the seedling basin, and when the top cover is taken away, it can automatically lift the seedling basin upward, so that the liquid medicine entering the seedling basin and attached to the roots of the seedlings can be drained, without the need for manual hand-held seedling basket to drain the water, saving manpower and improving work efficiency.

[0054] The docking unit includes a docking joint 15 and an intermediate docking column 16, wherein the intermediate docking column 16 is movably arranged in a through hole (not marked), the docking joint 15 is coaxially fixed at one end of the docking joint 16, and the docking joint 15 and the inner end of the rotating pin 13 are movably engaged to transmit torque, a docking slot (not marked) is provided on the other side of the intermediate docking column 16, a mounting slot 6 is provided on the side of the support plate 5, a connecting shaft 8 is movably arranged in the mounting slot 6, the other end of the connecting shaft 8 is used to engage with the docking slot to transmit torque, and the cross-section of the mounting slot 6 and the cross-section of the connecting shaft 8 are regular polygons, so that the two can cooperate to transmit torque. Through the above structure, the installation and disassembly between the support plate 5 and the two clamping blocks 12 can be facilitated.

[0055] The inner wall of the through hole is provided with a plurality of limit grooves 10 along the circumferential direction, and the side wall of the rotating pin 13 is provided with a plurality of elastic pins 11 which are slidably matched with the limit grooves 10. With the above structure, the rotating pin 13 can be conveniently installed and removed.

[0056] The end of the limiting groove 10 close to the middle docking column 16 is provided with a rounded corner 9, and the side of the middle docking column 16 away from the connecting pin 13 has a tapered slope 17. It is convenient to guide the elastic pin to retract and facilitate the disassembly of the connecting pin 13.

[0057] The rotating pin 13 and the middle docking column 16 are magnetically mutually repelled, and the inner end of the rotating pin 13 is provided with a slot for engaging with the docking head 15. In this way, the middle docking column 16 can be stably connected to the connecting shaft 8, so that the supporting plate 5 drives the connecting pin 13 to rotate in the through hole.

[0058] The clamp block 12 has a protrusion on one side close to the support plate 5, and a limit plate 7 is provided on the part of the connecting shaft 8 outside the installation slot 6, so that the supporting plate 5 is close to the clamp block 12, and the protrusion abuts against the limit plate 7 to separate the connecting shaft 8 and the docking slot.

[0059] The above structure makes disassembly more convenient, so that the connecting shaft 8 and the docking slot on the middle docking column can be quickly separated, making it easy to replace the support plate 5.

[0060] Embodiment 2:

[0061] Reference Figure 8 In the present embodiment, in the elastic mechanism on the two opposite sides of the inner wall of the medicine basin 2, the elastic force of the torsion spring 14 on which the rotating pin 13 is installed is different, so that in specific use, when the top cover 4 is removed, the elastic supporting mechanism can make the seedling basket 3 automatically rise to a certain height, and finally make its bottom surface height higher than the liquid level in the medicine basin 2, and because of the different elastic forces of the torsion springs in the elastic mechanism, the seedling basket 3 has a certain inclination, which can improve its drainage effect.

[0062] The above-mentioned embodiments are only preferred embodiments of the present invention and cannot be used to limit the scope of protection of the present invention. Any non-substantial changes and substitutions made by technicians in this field on the basis of the present invention shall fall within the scope of protection required by the present invention.

Claims

1. A large-scale and easy-to-operate haploid chemical doubling device, It is characterized in that include A seedling basket, which is used to hold seedlings; Medicine basin, which is used to store medicine solution and place seedling basket The bottom basin is used to place the medicine pot and the seedling basket, and A top cover, which is used to seal the opening of the bottom basin; The inner wall of the medicine holding basin is provided with a plurality of elastic support mechanisms distributed along the circumferential direction, and the elastic support mechanisms are pressed down when the top rod cooperates with the bottom basin; The top cover is provided with a plurality of groups of clamping units clamped with the top of the bottom basin, and the top opening of the bottom basin is provided with a flange facing outward, and the clamping units are clamped with the bottom of the flange; The clamping unit includes a movable hook and a connecting pin, wherein one end of the movable hook is rotatably connected to the top cover through the connecting pin, a return spring is installed on the connecting pin, and the other end of the movable hook is provided with a hook head clamped with the bottom of the flange; The elastic support mechanism comprises a support plate and two clamping blocks, wherein the two clamping blocks are respectively fixed to the inner wall of the medicine holding basin, and through holes are opened in the two clamping blocks, and a rotating pin is installed at the through hole, one end of the rotating pin extends out of the through hole and is installed with a torsion spring, and a docking unit cooperating with the rotating pin is also installed in the through hole, and one side of the support plate is located between the two clamping blocks and is detachably connected to the docking units on the two clamping blocks; The docking unit includes a docking joint and an intermediate docking column, wherein the intermediate docking column is movably arranged in the through hole, the docking joint is coaxially fixed at one end of the docking column, and the docking joint and the inner end of the rotating pin shaft are movably engaged to transmit torque, a docking slot is provided on the other side of the intermediate docking column, a mounting slot is provided on the side of the support plate, a connecting shaft is movably provided in the mounting slot, and the other end of the connecting shaft is used to engage with the docking slot to transmit torque, and the cross-section of the mounting slot and the cross-section of the connecting shaft are regular polygons, so that the two can transmit torque when they cooperate.

2. A large-scale and easy-to-operate haploid chemical doubling device as claimed in claim 1, It is characterized in that The inner wall of the through hole is provided with a plurality of limiting grooves along the circumferential direction, and the side wall of the rotating pin shaft is provided with a plurality of elastic pins which are slidably matched with the limiting grooves.

3. A large-scale and easy-to-operate haploid chemical doubling device as claimed in claim 1, It is characterized in that The end of the limiting groove close to the middle docking column is provided with a rounded corner, and the side of the middle docking column away from the connecting pin shaft has a conical slope surface.

4. A large-scale and easy-to-operate haploid chemical doubling device as claimed in claim 1, It is characterized in that The rotating pin shaft and the middle docking column are magnetically mutually repelled, and the inner end of the rotating pin shaft is provided with a slot for clamping with the docking head.

5. A large-scale and easy-to-operate haploid chemical doubling device as claimed in claim 1, It is characterized in that The clamp block has a protrusion on one side close to the support plate, and a limit plate is provided on the part of the connecting shaft outside the installation slot, and the supporting plate is made close to the clamp block, and the protrusion abuts against the limit plate to separate the connecting shaft and the docking slot.

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