Optimization method and device for cleaning reagent needle and biochemical analyzer

Abstract

The application discloses a reagent needle cleaning optimization method and device and a biochemical analyzer, wherein the method comprises the following steps: defining the pollution types corresponding to each reagent, wherein the pollution types comprise a pollution source and a polluted source; judging whether the required reagent comprises the pollution source reagent according to test item information, and if the pollution source reagent is included, performing one-time strengthened cleaning on the reagent needle after the pollution source reagent is injected by the reagent needle. The technical scheme provided by the application case reduces the pollution carrying amount of the reagent needle, avoids the problem of possible pollution carrying of the next reagent, optimizes the test process, makes the test result more accurate, simultaneously avoids increasing unnecessary cleaning times, and ensures the test efficiency.

Description

TECHNICAL FIELD

[0001] The embodiment of the present application relates to the technical field of biochemical analyzers, in particular to a reagent needle cleaning optimization method and device and a biochemical analyzer. BACKGROUND

[0002] The reagent needle of a biochemical analyzer has various types, and a single-needle scheme of a common reagent needle has attracted attention due to miniaturization and low cost. In the single-needle scheme, a sample, a reagent, and their injection sites and cleaning pools are arranged in a straight line, and the reagent needle is cleaned in and out after injecting the sample and the reagent to complete the cleaning of the inner and outer walls of the needle, so as to complete the injection of the sample and multiple reagents and the cleaning of the needle in one cycle.

[0003] However, due to the limitation of the cleaning time, the sample injection process is alternated between the sample and the reagent, and the cleaning is not thorough in the effective cleaning time, and the residual inner and outer walls of the needle lead to the carryover pollution of the reagent needle or the cross-contamination between reagent bottles, so that the authenticity of the detection result is easily affected or interfered. In the prior art, cleaning is increased before each sampling of the reagent needle to reduce the carryover pollution, which undoubtedly increases the test time and reduces the test efficiency. SUMMARY

[0004] The present application provides a reagent needle cleaning optimization method and device and a biochemical analyzer, which reduces the carryover pollution amount of the reagent needle, avoids the problem of possible carryover pollution of the next reagent, optimizes the test process, makes the test result more accurate, and also avoids increasing unnecessary cleaning times, thereby ensuring the test efficiency.

[0005] In a first aspect, the embodiment of the present application provides a reagent needle cleaning optimization method, which comprises the following steps.

[0006] Defining the pollution types corresponding to each reagent, wherein the pollution types include a pollution source reagent and a contaminated source reagent;

[0007] According to the test item information, it is judged whether the required reagent includes the pollution source reagent, if not, the reagent needle is cleaned once after injecting the contaminated source reagent; if yes, the reagent needle is cleaned once after injecting the pollution source reagent, and then cleaned again.

[0008] Optionally, after judging whether the required reagent includes the pollution source reagent according to the test item information, the following steps are further included.

[0009] Verifying the carryover pollution rate of the inner wall of the reagent needle;

[0010] Verifying the carryover pollution rate of the outer wall of the reagent needle;

[0011] The optimization method is evaluated based on the test results of the carryover rate of the inner wall of the reagent needle and the carryover rate of the outer wall of the reagent needle.

[0012] Optionally, the carryover rate of the inner wall of the reagent needle is verified, including:

[0013] The initial absorbance of the source reagent and the initial absorbance of the reference reagent are obtained;

[0014] The source reagent and the reference reagent are alternately injected into the cuvettes through the reagent needle, wherein after the injection of the source reagent is completed, the reagent needle is subjected to the regular cleaning and the enhanced cleaning once;

[0015] The first average of the absorbance of each cuvette corresponding to the reference reagent is counted;

[0016] The carryover rate of the inner wall of the reagent needle is obtained according to the initial absorbance of the source reagent, the initial absorbance of the reference reagent and the first average.

[0017] Optionally, the carryover rate of the inner wall of the reagent needle is obtained according to the initial absorbance of the source reagent, the initial absorbance of the reference reagent and the first average, including:

[0018] The carryover rate of the inner wall of the reagent needle is obtained according to a first formula, the first formula being:

[0019] The carryover rate of the inner wall of the reagent needle = (E1-E0) / E;

[0020] Wherein, E1 is the first average, E0 is the initial absorbance of the reference reagent, and E is the initial absorbance of the source reagent.

[0021] Optionally, the carryover rate of the outer wall of the reagent needle is verified, including:

[0022] The initial absorbance of the source reagent is obtained;

[0023] The source reagent and the reference reagent are alternately injected into the cuvettes through the reagent needle, wherein after the injection of the source reagent is completed, the reagent needle is subjected to the regular cleaning and the enhanced cleaning once;

[0024] The first average of the absorbance of each cuvette corresponding to the reference reagent is counted;

[0025] The remaining reference reagent is added into the cuvettes and the second average of the absorbance of each cuvette corresponding to the reference reagent is counted;

[0026] According to the initial absorbance of the pollution source reagent, the first average value, the second average value and the number of dispensing, the carrying pollution rate of the outer wall of the reagent needle is obtained.

[0027] Optionally, according to the initial absorbance of the pollution source reagent, the first average value, the second average value and the number of dispensing, the carrying pollution rate of the outer wall of the reagent needle is obtained, comprising:

[0028] According to a second formula, the carrying pollution rate of the outer wall of the reagent needle is obtained, the second formula being:

[0029] The carrying pollution rate of the outer wall of the reagent needle is (E2 / n-E1) / E.

[0030] Wherein, E1 is the first average value, E2 is the second average value, E is the initial absorbance of the pollution source reagent, and n is the number of dispensing.

[0031] Optionally, the p-nitrophenol pigment solution is selected to replace the pollution source reagent, and the initial absorbance of the pollution source reagent and the initial absorbance of the reference reagent are obtained, comprising:

[0032] The p-nitrophenol pigment solution is diluted according to the absorbance of the pollution source reagent, and a theoretical absorbance of the p-nitrophenol pigment solution is obtained, which is used as the initial absorbance of the reagent.

[0033] The absorbance of the reference reagent is measured multiple times, and an average absorbance of the reference reagent is obtained, which is used as the initial absorbance of the reference reagent.

[0034] In a second aspect, the embodiments of the present application further provide a reagent needle cleaning optimization device, comprising:

[0035] A definition unit is configured to define pollution types corresponding to each reagent, wherein the pollution types include pollution source reagents and contaminated source reagents.

[0036] A judgment unit is configured to determine whether the required reagents include the pollution source reagents according to test item information, and if the pollution source reagents are not included, the reagent needle is subjected to a regular cleaning after dispensing the contaminated source reagents; if the pollution source reagents are included, the reagent needle is subjected to a regular cleaning and then a strengthened cleaning after dispensing the pollution source reagents.

[0037] Optionally, the reagent needle cleaning optimization device further comprises a verification unit configured to verify the carrying pollution rate of the inner wall of the reagent needle and the carrying pollution rate of the outer wall of the reagent needle, and evaluate the optimization method of reagent needle cleaning based on the test results of the carrying pollution rate of the inner wall of the reagent needle and the carrying pollution rate of the outer wall of the reagent needle.

[0038] In a third aspect, the embodiments of the present application also provide a biochemical analyzer, which comprises the reagent needle cleaning optimization device according to any of the embodiments of the present application.

[0039] The embodiments of the present application define the pollution types of each reagent, determine the required reagent according to the test item information, insert a strengthened cleaning process after the reagent needle is cleaned regularly after dispensing the reagent corresponding to the pollution source and before the next reagent is sucked if the pollution source reagent is included in the test item, thereby reducing the pollution carrying amount of the reagent needle, avoiding the problem of possible pollution carrying of the next reagent, optimizing the test process, making the test result more accurate, and also avoiding increasing unnecessary cleaning times, and ensuring the test efficiency. BRIEF DESCRIPTION OF DRAWINGS

[0040] Figure 1 A flowchart of a reagent needle cleaning optimization method provided by the embodiments of the present application;

[0041] Figure 2 A test flowchart of the carrying pollution rate of the inner wall of a reagent needle provided by the embodiments of the present application;

[0042] Figure 3 A test flowchart of the carrying pollution rate of the outer wall of a reagent needle provided by the embodiments of the present application;

[0043] Figure 4 A structural diagram of a reagent needle cleaning control device provided by the embodiments of the present application. DETAILED DESCRIPTION

[0044] To make the objectives, technical solutions and advantages of the embodiments of the present application clearer, the technical solutions in the embodiments of the present application will be described below in conjunction with the drawings of the embodiments of the present application. Obviously, the described embodiments are some but not all of the embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative work fall within the protection scope of the present application.

[0045] Figure 1 A flowchart of a reagent needle cleaning optimization method provided by the embodiments of the present application, which can be applicable to the cleaning of a reagent needle of a biochemical analyzer. The method can be executed by a reagent needle cleaning control device, which can be realized in the form of hardware and / or software. The method specifically comprises the following steps:

[0046] S110, define the pollution types corresponding to each reagent, including pollution source reagents and reagents polluted by the pollution source;

[0047] Specifically, due to different solution concentrations of the reagents, after the reagent needle sucks the corresponding reagent, the wall hanging pollution degree of the inner and outer walls of the reagent needle is different, so the pollution degree between the reagent needle and the sample and the reagent is different. According to the actual type of each reagent, the mapping relationship between the reagent and the pollution type of the reagent needle is obtained through the accumulation of production experience or data experimental measurement, so as to match the pollution type of each reagent.

[0048] S120, according to the test item information, it is judged whether the required reagent includes a pollution source reagent, if not, the reagent needle is cleaned once after the pollution source reagent is injected; if it includes a pollution source reagent, the reagent needle is cleaned once after the pollution source reagent is injected, and then it is cleaned once.

[0049] Specifically, the conventional cleaning is an effective cleaning process in the prior art, and the enhanced cleaning has a larger cleaning intensity than the conventional cleaning, for example, by increasing the cleaning time, the cleaning liquid flow rate and the like to improve the cleaning intensity. The test item information includes the required reagent, reagent combination and test timing and the like. If it is judged according to the test item information that the current test item does not carry a pollution source reagent, the order of the reagent needle sucking the project is performed according to the order of the sample test. For example, in order to reduce the cross contamination between the reagents, a conventional cleaning can be performed between each injection of the reagent needle. If it is judged according to the test item information that the current test item includes a pollution source reagent, the reagent needle does not suck the corresponding reagent after completing the pollution source reagent injection, but inserts an enhanced cleaning process after the conventional cleaning, thereby reducing the pollution carrying amount of the reagent needle, avoiding the possible carrying pollution problem of the next reagent, optimizing the test process, making the test result more accurate, and at the same time, increasing the cleaning times in a targeted manner, avoiding unnecessary cleaning times, and ensuring the test efficiency.

[0050] The embodiment of the application defines the pollution type of each reagent, and determines the required reagent according to the test item information. If the test item includes a pollution source reagent, the reagent needle is cleaned once after injecting the reagent corresponding to the pollution source, and then an enhanced cleaning process is inserted before sucking the next reagent, thereby reducing the pollution carrying amount of the reagent needle, avoiding the possible carrying pollution problem of the next reagent, optimizing the test process, making the test result more accurate, and at the same time, avoiding unnecessary cleaning times, and ensuring the test efficiency.

[0051] Optionally, after judging whether the required reagent includes a pollution source reagent according to the test item information, the following steps are further included:

[0052] Verify the carrying pollution rate of the inner wall of the reagent needle;

[0053] Verify the carrying pollution rate of the outer wall of the reagent needle;

[0054] The optimization method is evaluated based on the test results of the carryover contamination rate of the inner wall of the reagent needle and the carryover contamination rate of the outer wall of the reagent needle.

[0055] Specifically, after the reagent needle completes dispensing, a certain amount of reagent solution remains on the inner wall and the outer wall of the reagent needle, and thus the carryover contamination rate of the inner wall and the outer wall of the reagent needle after the reagent needle completes dispensing can be obtained through quantitative verification, that is, when at least one of the test results of the carryover contamination rate of the inner wall of the reagent needle and the carryover contamination rate of the outer wall of the reagent needle does not meet the contamination acceptance standard, it is considered to be contaminated, and thus the actual effect of the optimization method can be evaluated by verifying the test results of the carryover contamination rate of the inner wall of the reagent needle and the carryover contamination rate of the outer wall of the reagent needle. For example, a certain verification period is set, and the carryover contamination rate of the reagent needle is verified regularly to correct or remind the cleaning result of the reagent needle in time, so that the test project maintains good accuracy.

[0056] Figure 2 A test flowchart of the carryover contamination rate of the inner wall of the reagent needle provided by the embodiment of the present application is shown in FIG. 1. Figure 2 , comprising:

[0057] S210, obtaining the initial absorbance of the contamination source reagent and the initial absorbance of the reference reagent;

[0058] Specifically, when the carryover contamination of the reagent needle is quantitatively measured, a high-concentration p-nitrophenol (PNP) pigment solution can be used to replace the contamination source reagent, which can avoid excessive consumption of the contamination source reagent, save test cost, and the reference reagent is purified water. The contaminated condition of the purified water is more harsh, and compared with direct use of detection, the pollution data is easier to detect, and thus the purified water can be considered as the contamination source reagent. For example, a suitable high-concentration PNP pigment solution is selected to replace the contamination source reagent, a cleaning liquid acid or a cleaning liquid is selected as an intermediate process cleaning liquid, and purified water is selected as the reference reagent for testing. The high-concentration PNP solution is diluted and the initial absorbance is measured to obtain the initial absorbance of the current measurement reagent. Similarly, the reference reagent is measured for absorbance to obtain the initial absorbance of the reference reagent.

[0059] S220, the contamination source reagent and the reference reagent are alternately dispensed into the cuvette through the reagent needle, wherein after the contamination source reagent is dispensed, the reagent needle is subjected to one regular cleaning and one enhanced cleaning;

[0060] Specifically, the PNP solution and the test reagent are alternately dispensed into the cuvettes, and the volume of each dispensing can be the same. After dispensing the PNP solution once, the reagent needle is cleaned once normally and once intensively, maintaining the same cleaning mode as after the reagent needle absorbs the contaminated source reagent to maintain the same experimental conditions. After cleaning, purified water is dispensed, and the alternating dispensing is performed at least 5 times or more to avoid data errors caused by too few data.

[0061] S230, statistics of the first mean value of the absorbance of each cuvette corresponding to the test reagent;

[0062] Specifically, after dispensing, the absorbance in each cuvette of the test reagent is measured, and the mean value thereof is calculated as the first mean value.

[0063] S240, obtaining the carry contamination rate of the inner wall of the reagent needle according to the initial absorbance of the source reagent, the initial absorbance of the test reagent, and the first mean value.

[0064] Specifically, the increased absorbance in the cuvette of the test reagent is caused by the reagent solution carried by the inner wall of the reagent needle, so the increase in absorbance is obtained according to the initial absorbance of the test reagent and the first mean value, and the carry contamination rate of the inner wall of the reagent needle is obtained according to the increase and the initial absorbance of the reagent. For example, the carry contamination rate of the inner wall of the reagent needle is obtained according to the initial absorbance of the source reagent, the initial absorbance of the test reagent, and the first mean value, including:

[0065] The carry contamination rate of the inner wall of the reagent needle is obtained according to the first formula, and the first formula is:

[0066] The carry contamination rate of the inner wall of the reagent needle = (E1-E0) / E;

[0067] Wherein, E1 is the first mean value, E0 is the initial absorbance of the test reagent, and E is the initial absorbance of the source reagent.

[0068] Figure 3 A test flowchart of the carry contamination rate of the outer wall of the reagent needle is provided for the embodiments of the present application, referring to Figure 3 , including:

[0069] S310, obtaining the initial absorbance of the source reagent;

[0070] For example, the embodiments of the present application select a suitable high-concentration PNP pigment solution to replace the source reagent, select an acid or a cleaning liquid as the intermediate process cleaning liquid, and select purified water as the test reagent for testing. The high-concentration PNP solution is diluted, and the initial absorbance is measured by absorbance measurement, which is used as the initial absorbance of the current measured reagent.

[0071] S320, alternately dispensing the pollution source reagent and the reference reagent into the cuvettes through the reagent needle, wherein, after the dispensing of the pollution source reagent is completed, the reagent needle is cleaned once regularly and once intensively;

[0072] Specifically, the PNP solution and the reference reagent are alternately dispensed into the cuvettes, wherein the volume of each dispensing can be the same. After the dispensing of the PNP solution is completed once, the reagent needle is cleaned once regularly and once intensively, and the same cleaning mode as that after the reagent needle sucks the pollution source reagent is maintained to maintain the same experimental conditions. After the cleaning is completed, the dispensing of the purified water is performed again, wherein the dispensing is alternated for at least 5 times or more to avoid data errors caused by too few data. For example, each of the embodiments of the present application is dispensed for 10 times, and a total of 20 times of dispensing is performed.

[0073] S330, calculating a first average of the absorbance of each cuvette corresponding to the reference reagent;

[0074] Specifically, after the dispensing is completed, the absorbance in each cuvette of the reference reagent is measured, and the average is calculated as the first average.

[0075] S340, adding the remaining reference reagent into the cuvettes and calculating a second average of the absorbance of each cuvette corresponding to the reference reagent;

[0076] Specifically, the remaining reference reagent is mixed, the mixed reference reagent is added into the cuvettes corresponding to the reference reagent, for example, the dispensing is performed in the corresponding 1-10 cuvettes, and the absorbance is measured respectively to calculate the corresponding average, which is recorded as the second average.

[0077] S350, obtaining the carrying pollution rate of the outer wall of the reagent needle according to the initial absorbance of the pollution source reagent, the first average, the second average, and the number of dispensing.

[0078] Specifically, in each process of sucking the reference reagent, the increase in the absorbance of the reference reagent can be considered as the reagent solution carried by the outer wall of the reagent needle, and thus the ratio of the second average to the number of dispensing is the absorbance carried by the outer wall of each dispensing. The increase in the absorbance is obtained according to the absorbance carried by the outer wall of each dispensing and the first average, and the carrying pollution rate of the outer wall of the reagent needle is obtained according to the increase and the initial absorbance of the reagent. Alternatively, the carrying pollution rate of the outer wall of the reagent needle is obtained according to the initial absorbance of the pollution source reagent, the first average, the second average, and the number of dispensing, including:

[0079] The carrying pollution rate of the outer wall of the reagent needle is obtained according to the second formula, and the second formula is:

[0080] The carrying pollution rate of the outer wall of the reagent needle = (E2 / n-E1) / E;

[0081] Wherein, E1 is the first average, E2 is the second average, E is the initial absorbance of the pollution source reagent, and n is the number of dispensing. That is, when n is 10, the carrying pollution rate of the outer wall of the reagent needle is (E2 / 10-E1) / E.

[0082] Optionally, the p-nitrophenol pigment solution is selected to replace the pollution source reagent to obtain the initial absorbance of the pollution source reagent and the initial absorbance of the reference reagent, and the method comprises the following steps of:

[0083] The p-nitrophenol pigment solution is diluted according to the absorbance of the pollution source reagent, and the theoretical absorbance of the p-nitrophenol pigment solution is obtained, and the theoretical absorbance is taken as the initial absorbance of the reagent.

[0084] The absorbance of the reference reagent is measured multiple times, and the average absorbance of the reference reagent is obtained, and the average absorbance of the reference reagent is taken as the initial absorbance of the reference reagent.

[0085] Specifically, a high-concentration p-nitrophenol (PNP) solution is used to replace the pollution source reagent, which can avoid excessive consumption of reagents, save testing costs, and the reference reagent is purified water. The pollution condition of the purified water is more harsh, and compared with direct detection, the pollution data is easier to detect. The absorbance of the high-concentration PNP solution is diluted to 1-2 Abs, the real absorbance of the diluted solution is measured by an instrument, and the dilution multiple is multiplied, that is, the theoretical absorbance of the PNP solution, and the theoretical absorbance is recorded as the initial absorbance of the reagent. The reference reagent is placed in the reaction cup, and the absorbance is measured multiple times, and the average is calculated as the initial absorbance of the reference reagent.

[0086] Optionally, the test result evaluation optimization method based on the carrying pollution rate of the inner wall of the reagent needle and the carrying pollution rate of the outer wall of the reagent needle comprises the following steps of:

[0087] If the carrying pollution rate of the inner wall of the reagent needle and / or the carrying pollution rate of the outer wall of the reagent needle meets the pollution acceptance standard, it indicates that the optimization method can well complete the cleaning of the reagent needle. Specifically, considering the pollution acceptance standard of the reagent needle, if the carrying pollution rate of the inner wall of the reagent needle and / or the carrying pollution rate of the outer wall of the reagent needle meets (is less than) the pollution acceptance standard after the reagent needle completes the reagent dispensing, it indicates that the optimization method can well complete the cleaning of the reagent needle, and if the carrying pollution rate of the inner wall of the reagent needle and / or the carrying pollution rate of the outer wall of the reagent needle does not meet (is greater than) the pollution acceptance standard after the reagent needle completes the reagent dispensing, it indicates that the optimization method cannot complete the cleaning of the reagent needle, and needs to be alarmed or optimized.

[0088] Figure 4 A control device for cleaning the reagent needle is provided for the embodiment of the present application, and a structural schematic diagram of the control device is shown in Figure 4 , which comprises the following steps of:

[0089] The definition unit 110 is configured to define the pollution type corresponding to each reagent, and the pollution type includes a pollution source reagent and a contaminated source reagent.

[0090] The judgment unit 120 is configured to determine, according to the test item information, whether the required reagent includes the pollution source reagent. If the required reagent does not include the pollution source reagent, the reagent needle is subjected to a regular cleaning after the reagent needle dispenses the contaminated source reagent. If the required reagent includes the pollution source reagent, the reagent needle is subjected to a regular cleaning and then a strengthened cleaning after the reagent needle dispenses the pollution source reagent.

[0091] Specifically, because the solution concentrations of the reagents are different, the wall hanging pollution degrees of the inner and outer walls of the reagent needle are different after the reagent needle sucks the corresponding reagent, and thus the pollution degrees between the reagent needle and the sucked sample and reagent are different. According to the actual types of the reagents, the mapping relationship between the reagents and the pollution types of the reagent needle is obtained through accumulation of production experience or data experimental measurement, and the definition unit 110 matches the pollution types of the reagents.

[0092] The regular cleaning is an effective cleaning process in the prior art, and the strengthened cleaning has a larger cleaning intensity than the regular cleaning, for example, the cleaning intensity is increased by increasing the cleaning time, the cleaning liquid flow rate, or the like. The test item information includes information such as required reagents, reagent combinations, and test timing. If the judgment unit 120 determines, according to the test item information, that the current test item does not carry the pollution source reagent, the order of the reagent needle sucking the items is performed according to the order of sample testing. For example, in order to reduce the cross contamination between the reagents, the reagent needle is subjected to a regular cleaning between each dispensing. If the judgment unit 120 determines, according to the test item information, that the current test item includes the pollution source reagent, the reagent needle does not suck the corresponding reagent after completing the pollution source reagent dispensing, but inserts a strengthened cleaning process after the regular cleaning, thereby reducing the pollution carrying amount of the reagent needle, avoiding the problem of possibly carrying pollution to the next reagent, optimizing the test process, making the test result more accurate, and simultaneously increasing the cleaning times in a targeted manner, avoiding unnecessary increase in the cleaning times, and ensuring the test efficiency.

[0093] The embodiment of the application defines the pollution types of the reagents through the definition unit, the judgment unit determines the required reagents according to the test item information, if the test item includes the pollution source reagent, the reagent needle is subjected to a regular cleaning and then a strengthened cleaning process after dispensing the pollution source reagent and before sucking the next reagent, thereby reducing the pollution carrying amount of the reagent needle, avoiding the problem of possibly carrying pollution to the next reagent, optimizing the test process, making the test result more accurate, and simultaneously avoiding unnecessary increase in the cleaning times, and ensuring the test efficiency.

[0094] Table 1 is the reagent needle carrying pollution rate before optimization

[0095] Verification content Reagent needle carrying pollution Pollution acceptance standard Whether qualified Reagent needle inner wall carrying pollution rate 1.11 x 10 -5 ]] 1.00 x 10 -7 ]]> Unqualified Reagent needle outer wall carrying pollution rate 8.42 x 10 -6 ]] 1.00 x 10 -7 ]]> Unqualified

[0096] Table 2 is the reagent needle carrying pollution rate after optimization

[0097] Verification content Reagent needle carrying pollution Pollution acceptance standard Whether qualified Reagent needle inner wall carrying pollution rate 5.29 x 10 -8 ]] 1.00 x 10 -7 ]]> Qualified Reagent needle outer wall carrying pollution rate 9.63 x 10 -8 ]] 1.00 x 10 -7 ]] Qualified

[0098] From the data results, by setting the reagent needle cleaning control optimization, the carrying pollution rate is improved by about 2-3 orders of magnitude than that without setting, and the ability to prevent carrying pollution is greatly improved.

[0099] Optionally, the reagent needle cleaning optimization device further comprises a verification unit configured to verify the carrying pollution rate of the inner wall of the reagent needle and the carrying pollution rate of the outer wall of the reagent needle, and evaluate the optimization method of the reagent needle cleaning based on the test results of the carrying pollution rate of the inner wall of the reagent needle and the carrying pollution rate of the outer wall of the reagent needle.

[0100] Optionally, the verification unit comprises:

[0101] The acquisition sub-unit is configured to acquire the initial absorbance of the pollution source reagent and the initial absorbance of the reference reagent.

[0102] The dispensing sub-unit is configured to alternately dispense the pollution source reagent and the reference reagent into the cuvettes through the reagent needle, wherein after the dispensing of the pollution source reagent is completed, the reagent needle is subjected to one regular cleaning and one enhanced cleaning.

[0103] The statistical sub-unit is configured to statistically acquire the first mean value of the absorbance of each cuvette corresponding to the reference reagent.

[0104] The calculation sub-unit is configured to acquire the carrying pollution rate of the inner wall of the reagent needle according to the initial absorbance of the pollution source reagent, the initial absorbance of the reference reagent, and the first mean value.

[0105] Optionally, the calculation sub-unit acquires the carrying pollution rate of the inner wall of the reagent needle according to a first formula, and the first formula is:

[0106] The carrying pollution rate of the inner wall of the reagent needle = (E1-E0) / E.

[0107] Wherein, E1 is the first mean value, E0 is the initial absorbance of the reference reagent, and E is the initial absorbance of the pollution source reagent.

[0108] Based on the above embodiment, the acquisition sub-unit is configured to acquire the initial absorbance of the pollution source reagent.

[0109] The dispensing sub-unit is configured to alternately dispense the pollution source reagent and the reference reagent into the cuvettes through the reagent needle, wherein after the dispensing of the pollution source reagent is completed, the reagent needle is subjected to one regular cleaning and one enhanced cleaning.

[0110] The statistical subunit is configured to count a first average of the absorbance of each cuvette corresponding to the test reagent;

[0111] The dispensing subunit is further configured to add the remaining test reagent into the cuvette.

[0112] The statistical subunit is further configured to count a second average of the absorbance of each cuvette corresponding to the test reagent.

[0113] The calculating subunit is further configured to obtain the carry-over contamination rate of the outer wall of the reagent needle according to the initial absorbance of the contamination source reagent, the first average, the second average and the number of dispensing.

[0114] Optionally, the calculating subunit obtains the carry-over contamination rate of the outer wall of the reagent needle according to a second formula, the second formula being:

[0115] The carry-over contamination rate of the outer wall of the reagent needle = (E2 / n-E1) / E.

[0116] Wherein, E1 is the first average, E2 is the second average, E is the initial absorbance of the contamination source reagent, and n is the number of dispensing.

[0117] The control device for cleaning the reagent needle provided by the embodiments of the present application and the optimization method for cleaning the reagent needle provided by any of the embodiments of the present application belong to the same inventive concept, have corresponding beneficial effects, and the technical details of the embodiments are described in detail in the optimization method for cleaning the reagent needle provided by any of the embodiments of the present application.

[0118] The embodiments of the present application further provide a biochemical analyzer comprising the optimization device for cleaning the reagent needle according to any of the embodiments of the present application. The optimization device is used to execute the optimization method for cleaning the reagent needle. Since the biochemical analyzer comprises the optimization device for cleaning the reagent needle according to any of the embodiments of the present application, it has the same beneficial effects as the optimization device and the optimization method for cleaning the reagent needle, which will not be described here.

[0119] Finally, it should be noted that: the above embodiments are only used to illustrate the technical solutions of the present application, but not to limit them; although the present application has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that: it can still modify the technical solutions recorded in the foregoing embodiments, or make equivalent replacement to part of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present application.

Claims

1. A method for optimizing reagent needle cleaning, characterized by, The method comprises the following steps: defining the pollution types corresponding to each reagent, wherein the pollution types include pollution source reagents and contaminated source reagents; judging whether the required reagents include the pollution source reagents according to the test item information, if not, performing a regular cleaning on the reagent needle after the reagent needle injects the contaminated source reagents, if yes, performing a regular cleaning and then a strengthened cleaning on the reagent needle after the reagent needle injects the pollution source reagents; after judging whether the required reagents include the pollution source reagents according to the test item information, further comprising the following steps: verifying the carry-over contamination rate of the inner wall of the reagent needle; verifying the carry-over contamination rate of the outer wall of the reagent needle; evaluating the optimization method based on the test results of the carry-over contamination rate of the inner wall of the reagent needle and the carry-over contamination rate of the outer wall of the reagent needle; wherein verifying the carry-over contamination rate of the inner wall of the reagent needle comprises the following steps: obtaining the initial absorbance of the pollution source reagent and the initial absorbance of the reference reagent; alternately injecting the pollution source reagent and the reference reagent into cuvettes through the reagent needle, wherein after the injection of the pollution source reagent is completed, performing a regular cleaning and a strengthened cleaning on the reagent needle; statistically obtaining the first average of the absorbance of each cuvette corresponding to the reference reagent; obtaining the carry-over contamination rate of the inner wall of the reagent needle according to the initial absorbance of the pollution source reagent, the initial absorbance of the reference reagent and the first average; verifying the carry-over contamination rate of the outer wall of the reagent needle comprises the following steps: obtaining the initial absorbance of the pollution source reagent; alternately injecting the pollution source reagent and the reference reagent into cuvettes through the reagent needle, wherein after the injection of the pollution source reagent is completed, performing a regular cleaning and a strengthened cleaning on the reagent needle; statistically obtaining the first average of the absorbance of each cuvette corresponding to the reference reagent; adding the remaining reference reagent into the cuvettes and statistically obtaining the second average of the absorbance of each cuvette corresponding to the reference reagent; obtaining the carry-over contamination rate of the outer wall of the reagent needle according to the initial absorbance of the pollution source reagent, the first average, the second average and the number of injections.

2. The method for optimizing reagent needle cleaning according to claim 1, characterized in that, obtaining the carry-over contamination rate of the inner wall of the reagent needle according to the initial absorbance of the pollution source reagent, the initial absorbance of the reference reagent and the first average comprises the following steps: obtaining the carry-over contamination rate of the inner wall of the reagent needle according to a first formula, wherein the first formula is: the carry-over contamination rate of the inner wall of the reagent needle=(E1-E0) / E; wherein E1 is the first average, E0 is the initial absorbance of the reference reagent and E is the initial absorbance of the pollution source reagent.

3. The method for optimizing reagent needle cleaning according to claim 1, wherein, obtaining the carry-over contamination rate of the outer wall of the reagent needle according to the initial absorbance of the pollution source reagent, the first average, the second average and the number of injections comprises the following steps: obtaining the carry-over contamination rate of the outer wall of the reagent needle according to a second formula, wherein the second formula is: the carry-over contamination rate of the outer wall of the reagent needle=(E2 / n-E1) / E; Wherein, E1 is the first mean value, E2 is the second mean value, E is the initial absorbance of the pollution source reagent, and n is the number of dispensing.

4. The method for optimizing reagent needle cleaning according to claim 1, wherein, The initial absorbance of the pollution source reagent and the initial absorbance of the reference reagent are obtained by replacing the pollution source reagent with a p-nitrophenol pigment solution, comprising: The p-nitrophenol pigment solution is diluted according to the absorbance of the pollution source reagent, and the theoretical absorbance of the p-nitrophenol pigment solution is obtained, which is used as the initial absorbance of the reagent; The absorbance of the reference reagent is measured multiple times, and the mean absorbance of the reference reagent is obtained, which is used as the initial absorbance of the reference reagent.

5. An apparatus for optimizing reagent needle cleaning, characterized by, Comprising: A definition unit is configured to define the pollution types corresponding to each reagent, wherein the pollution types include the pollution source reagent and the contaminated source reagent; A judgment unit is configured to determine whether the required reagent includes the pollution source reagent according to the test item information, and if the pollution source reagent is not included, the reagent needle is subjected to a regular cleaning after dispensing the contaminated source reagent; if the pollution source reagent is included, the reagent needle is subjected to a regular cleaning and then a strengthened cleaning after dispensing the pollution source reagent; The optimization device for cleaning the reagent needle further comprises a verification unit configured to verify the carry-over contamination rate of the inner wall of the reagent needle and the carry-over contamination rate of the outer wall of the reagent needle, and evaluate the optimization method for cleaning the reagent needle based on the test results of the carry-over contamination rate of the inner wall of the reagent needle and the carry-over contamination rate of the outer wall of the reagent needle; The verification unit comprises: An obtaining subunit is configured to obtain the initial absorbance of the pollution source reagent and the initial absorbance of the reference reagent; A dispensing subunit is configured to alternately dispense the pollution source reagent and the reference reagent into a cuvette through the reagent needle, wherein the reagent needle is subjected to a regular cleaning and a strengthened cleaning after dispensing the pollution source reagent; A statistical subunit is configured to statistically obtain the first mean value of the absorbance of each cuvette corresponding to the reference reagent; A calculation subunit is configured to obtain the carry-over contamination rate of the inner wall of the reagent needle according to the initial absorbance of the pollution source reagent, the initial absorbance of the reference reagent, and the first mean value; The dispensing subunit is further configured to add the remaining reference reagent into the cuvette; The statistical subunit is further configured to statistically obtain the second mean value of the absorbance of each cuvette corresponding to the reference reagent; The calculation subunit is further configured to obtain the carry-over contamination rate of the outer wall of the reagent needle according to the initial absorbance of the pollution source reagent, the first mean value, the second mean value, and the number of dispensing.

6. A biochemical analyzer characterized by comprising: The optimization device for cleaning the reagent needle of claim 5 is included.

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