A method for identifying drought resistance of Cyperus oleifera at sprout stage

Abstract

The invention relates to the technical field of drought resistance identification, and in particular to a method for identifying drought resistance of cyperus rotundus at the sprouting stage. The method for identifying drought resistance of cyperus rotundus at the sprouting stage provided by the invention comprises the following steps: firstly selecting cyperus rotundus to be identified, and disinfecting the cyperus rotundus, and then dividing the disinfected cyperus rotundus tubers into group A and group B, wherein group A is planted in arid soil, and group B is planted in soil with normal water content, recording the properties of cyperus rotundus at the sprouting stage, and calculating evaluation indexes according to the recorded properties of cyperus rotundus at the sprouting stage, and finally identifying drought resistance of cyperus rotundus at the sprouting stage according to a drought resistance evaluation system and the obtained evaluation indexes. The provided method can not only effectively reduce the risk of virus infection of cyperus rotundus during disinfection, but also improve the germination rate of cyperus rotundus; and also has high identification accuracy, and can achieve a rapid identification effect.

Description

Technical Field

[0001] The invention relates to the technical field of drought resistance identification, in particular to a method for identifying drought resistance of Cyperus oleifera at the sprout stage. Background Art

[0002] Cyperus oleiferus is an annual C4 plant of the Cyperaceae family. It is native to the Nile River Basin and the Mediterranean coast of Africa. It is an emerging economic crop that integrates food, oil and feed, has high comprehensive utilization value and great development potential. Cyperus oleiferus underground tubers are rich in nutrients such as starch (25% to 40%), oil (20% to 30%), sugar (15% to 20%), protein (5% to 10%), dietary fiber (8% to 10%), etc., and is known as the "king of oil crops". In addition, as a sand-dwelling plant, it has the characteristics of a well-developed root system, strong tillering ability, resistance to barrenness, and few diseases and insect pests. It is a high-quality windbreak and sand fixation and ecological restoration plant, and has important strategic significance for the development and utilization of marginal land such as desertified land, wasteland, and tidal flats.

[0003] In recent years, with the frequent global climate change and the increasing consumption of water resources, drought has become one of the main abiotic stresses restricting the growth and development of Cyperus oleifera. Therefore, it is urgent to carry out the screening and evaluation of drought resistance of Cyperus oleifera germplasm resources in a timely manner and then breed new drought-resistant varieties.

[0004] Therefore, the present invention provides a method for identifying drought resistance of Jatropha oleifera at the sprout stage, which is used to solve the above-mentioned related technical problems. Summary of the invention

[0005] The object of the present invention is to provide a method for identifying drought resistance of Cyperus oleifera at the sprouting stage. The method for identifying drought resistance of Cyperus oleifera at the sprouting stage provided by the present invention can not only effectively reduce the risk of virus infection of Cyperus oleifera during disinfection, but also improve the germination rate of Cyperus oleifera; and also has high identification accuracy and can achieve a rapid identification effect.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] The present invention provides a method for identifying drought resistance of Cyperus oleifera at the sprouting stage, comprising the following steps:

[0008] Ⅰ. Select the cyperus rotundus to be identified and disinfect it;

[0009] Ⅱ. The sterilized Cyperus oleifera tubers were divided into Group A and Group B. Group A was planted in arid soil, and Group B was planted in soil with normal moisture content. The characteristics of Cyperus oleifera during germination were recorded.

[0010] III. Calculate the evaluation index based on the recorded characteristics of germination period of Cyperus oleifera;

[0011] IV. Determine the drought resistance of Cyperus oleifera at the sprout stage based on the drought resistance evaluation system and the evaluation indicators obtained.

[0012] The present invention is further configured as follows: the Cyperus oleifera tubers selected to be identified in step I are plump Cyperus oleifera tubers of uniform size.

[0013] The present invention is further configured as follows: the disinfection process in step I is as follows:

[0014] Place the tubers of Cyperus oleifera in an appropriate amount of 0.5-1 mg / L chlorine dioxide disinfectant at a solid-liquid ratio of 0.01-0.02 g / mL, and soak them at 30-32°C for 5-7 minutes;

[0015] The soaked cyperus oleifera tubers are placed in a sealed container containing chlorine dioxide gas at a concentration of 0.012 to 0.015 g / mL and treated for 60 to 90 minutes at an air pressure of 40 to 60 kPa.

[0016] The present invention is further configured as follows: the moisture content of the dry soil in group A in step II is selected to be 5% to 10%.

[0017] The present invention is further configured as follows: the moisture content of the normal moisture content soil of group B in step II is selected to be 40% to 70%.

[0018] The present invention is further configured as follows: the properties of the germination period of the jatropha in step II include germination rate, bud length and root length.

[0019] The present invention is further configured as follows: the evaluation indexes in step III include relative germination rate, relative bud length, relative root length and storage material transport rate, wherein:

[0020] The calculation formula of the relative germination rate F is:

[0021] The calculation formula of the relative bud length Y is: Y = y A组 -y B组 , where y A组 ,y B组 is the average of the bud lengths of group A and group B;

[0022] The calculation formula of the relative root length D is: D = d A组 -d B组 , where d A组 d B组 is the average root length of group A and the average root length of group B;

[0023] The calculation formula of the storage material transport rate P is: In the formula, g A芽干重 is the average dry weight of group A buds, g A根干重 is the average dry weight of roots in group A, g B芽干重 is the average dry weight of group B buds, g B根干重 is the average dry weight of roots in group B.

[0024] The present invention is further configured as follows: the drought resistance evaluation system in step IV is an evaluation system with a set relative germination rate F as the first identification index, a set relative bud length Y and a relative root length D as the second identification index, and a set storage material transport rate P as the third identification index.

[0025] The present invention is further configured as follows: in step IV, if the identified Cyperus oleifera does not meet the set first identification index, it is a sensitive Cyperus oleifera; if the identified Cyperus oleifera meets the set first identification index but does not meet the set second identification index, it is a common Cyperus oleifera; if the identified Cyperus oleifera meets the set first identification index and the second identification index but does not meet the set third identification index, it is a relatively drought-resistant Cyperus oleifera; if the identified Cyperus oleifera meets the set first identification index, the second identification index and the third identification index, it is an excellent drought-resistant Cyperus oleifera.

[0026] Compared with the prior art, the present invention has the following beneficial effects:

[0027] The method for identifying drought resistance of cyperus rotundus at the sprouting stage provided by the present invention comprises the following steps: firstly selecting cyperus rotundus to be identified and disinfecting the cyperus rotundus, then dividing the disinfected cyperus rotundus tubers into group A and group B, wherein group A is planted in arid soil and group B is planted in soil with normal water content, recording the properties of cyperus rotundus at the sprouting stage, calculating evaluation indexes according to the recorded properties of cyperus rotundus at the sprouting stage, and finally identifying drought resistance of cyperus rotundus at the sprouting stage according to the drought resistance evaluation system and the obtained evaluation indexes; the provided method can not only effectively reduce the risk of virus infection of cyperus rotundus during disinfection, but also improve the germination rate of cyperus rotundus; and also has high identification accuracy, and can achieve a rapid identification effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0029] Figure 1 It is a statistical diagram of germination rate of the present invention;

[0030] Figure 2It is a statistical diagram of the accuracy of the present invention. DETAILED DESCRIPTION

[0031] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0032] Embodiment 1

[0033] This embodiment provides a method for identifying drought resistance of Cyperus oleifera at the sprout stage, comprising the following steps:

[0034] Ⅰ. Select the Cyperus rotundus to be identified and disinfect it.

[0035] Among them, the Cyperus oleifera tubers to be identified are plump and uniform in size.

[0036] The disinfection process is as follows:

[0037] The tubers of Cyperus oleifera were placed in an appropriate amount of 0.5 mg / L chlorine dioxide disinfectant at a solid-liquid ratio of 0.01 g / mL and immersed at 30°C for 5 min.

[0038] The soaked cyperus oleifera tubers were placed in a sealed container containing chlorine dioxide gas at a concentration of 0.012 g / mL and treated for 60 minutes at an air pressure of 40 kPa.

[0039] Ⅱ. The sterilized tubers of Cyperus oleifera were divided into Group A and Group B. Group A was planted in arid soil, and Group B was planted in soil with normal moisture content. The characteristics of Cyperus oleifera during germination were recorded.

[0040] Among them, the moisture content of the dry soil in Group A was selected to be 5%.

[0041] The moisture content of the normal moisture content soil of Group B was selected to be 40%.

[0042] The characteristics of germination period of jatropha include germination rate, shoot length and root length.

[0043] III. Calculate the evaluation index based on the recorded characteristics of germination period of Jatropha oleifera.

[0044] The evaluation indicators include relative germination rate, relative shoot length, relative root length and storage material transport rate, among which:

[0045] The calculation formula of relative germination rate F is:

[0046] The calculation formula of relative bud length Y is: Y = y A组 -y B组 , where y A组 ,y B组 is the average of the bud lengths of group A and group B;

[0047] The calculation formula of relative root length D is: D = d A组 -d B组 , where d A组 d B组 is the average root length of group A and the average root length of group B;

[0048] The calculation formula of storage material transport rate P is: In the formula, g A芽干重 is the average dry weight of group A buds, g A根干重 is the average dry weight of roots in group A, g B芽干重 is the average dry weight of group B buds, g B根干重 is the average dry weight of roots in group B.

[0049] IV. Determine the drought resistance of Cyperus oleifera at the sprout stage based on the drought resistance evaluation system and the evaluation indicators obtained.

[0050] The drought resistance evaluation system uses the set relative germination rate F as the first identification index, the set relative bud length Y and relative root length D as the second identification index, and the set storage material transport rate P as the third identification index.

[0051] Furthermore, if the identified Cyperus oleifera does not meet the set first identification index, it is a sensitive Cyperus oleifera; if the identified Cyperus oleifera meets the set first identification index but does not meet the set second identification index, it is a common Cyperus oleifera; if the identified Cyperus oleifera meets the set first and second identification indexes but does not meet the set third identification index, it is a relatively drought-resistant Cyperus oleifera; if the identified Cyperus oleifera meets the set first, second and third identification indexes, it is an excellent drought-resistant Cyperus oleifera.

[0052] Embodiment 2

[0053] This embodiment provides a method for identifying drought resistance of Cyperus oleifera at the sprout stage, comprising the following steps:

[0054] Ⅰ. Select the Cyperus rotundus to be identified and disinfect it.

[0055] Among them, the Cyperus oleifera tubers to be identified are plump and uniform in size.

[0056] The disinfection process is as follows:

[0057] The tubers of Cyperus oleifera were placed in an appropriate amount of 0.7 mg / L chlorine dioxide disinfectant at a solid-liquid ratio of 0.02 g / mL and immersed at 31°C for 6 min.

[0058] The soaked cyperus oleifera tubers were placed in a sealed container containing chlorine dioxide gas at a concentration of 0.013 g / mL and treated for 75 minutes at an air pressure of 50 kPa.

[0059] Ⅱ. The sterilized tubers of Cyperus oleifera were divided into Group A and Group B. Group A was planted in arid soil, and Group B was planted in soil with normal moisture content. The characteristics of Cyperus oleifera during germination were recorded.

[0060] Among them, the moisture content of the dry soil in Group A was selected to be 7%.

[0061] The moisture content of the normal moisture content soil of Group B was selected to be 55%.

[0062] The characteristics of germination period of jatropha include germination rate, shoot length and root length.

[0063] III. Calculate the evaluation index based on the recorded characteristics of germination period of Jatropha oleifera.

[0064] The evaluation indicators include relative germination rate, relative shoot length, relative root length and storage material transport rate, among which:

[0065] The calculation formula of relative germination rate F is:

[0066] The calculation formula of relative bud length Y is: Y = y A组 -y B组 , where y A组 ,y B组 is the average of the bud lengths of group A and group B;

[0067] The calculation formula of relative root length D is: D = d A组 -d B组 , where d A组 d B组 is the average root length of group A and the average root length of group B;

[0068] The calculation formula of storage material transport rate P is: In the formula, g A芽干重 is the average dry weight of group A buds, g A根干重 is the average dry weight of roots in group A, g B芽干重 is the average dry weight of group B buds, g B根干重 is the average dry weight of roots in group B.

[0069] IV. Determine the drought resistance of Cyperus oleifera at the sprout stage based on the drought resistance evaluation system and the evaluation indicators obtained.

[0070] The drought resistance evaluation system uses the set relative germination rate F as the first identification index, the set relative bud length Y and relative root length D as the second identification index, and the set storage material transport rate P as the third identification index.

[0071] Furthermore, if the identified Cyperus oleifera does not meet the set first identification index, it is a sensitive Cyperus oleifera; if the identified Cyperus oleifera meets the set first identification index but does not meet the set second identification index, it is a common Cyperus oleifera; if the identified Cyperus oleifera meets the set first and second identification indexes but does not meet the set third identification index, it is a relatively drought-resistant Cyperus oleifera; if the identified Cyperus oleifera meets the set first, second and third identification indexes, it is an excellent drought-resistant Cyperus oleifera.

[0072] Embodiment 3

[0073] This embodiment provides a method for identifying drought resistance of Cyperus oleifera at the sprout stage, comprising the following steps:

[0074] Ⅰ. Select the Cyperus rotundus to be identified and disinfect it.

[0075] Among them, the Cyperus oleifera tubers to be identified are plump and uniform in size.

[0076] The disinfection process is as follows:

[0077] The tubers of Cyperus oleifera were placed in an appropriate amount of 1 mg / L chlorine dioxide disinfectant at a solid-liquid ratio of 0.02 g / mL and immersed at 32°C for 7 min.

[0078] The soaked cyperus oleifera tubers were placed in a sealed container containing 0.015 g / mL of gaseous chlorine dioxide and treated for 90 minutes at an air pressure of 60 kPa.

[0079] Ⅱ. The sterilized tubers of Cyperus oleifera were divided into Group A and Group B. Group A was planted in arid soil, and Group B was planted in soil with normal moisture content. The characteristics of Cyperus oleifera during germination were recorded.

[0080] Among them, the moisture content of the dry soil in Group A was selected to be 10%.

[0081] The moisture content of the normal moisture content soil of Group B was selected to be 70%.

[0082] The characteristics of germination period of jatropha include germination rate, shoot length and root length.

[0083] III. Calculate the evaluation index based on the recorded characteristics of germination period of Jatropha oleifera.

[0084] The evaluation indicators include relative germination rate, relative shoot length, relative root length and storage material transport rate, among which:

[0085] The calculation formula of relative germination rate F is:

[0086] The calculation formula of relative bud length Y is: Y = y A组 -y B组 , where y A组 ,y B组 is the average of the bud lengths of group A and group B;

[0087] The calculation formula of relative root length D is: D = d A组 -d B组 , where d A组 d B组 is the average root length of group A and the average root length of group B;

[0088] The calculation formula of storage material transport rate P is: In the formula, g A芽干重 is the average dry weight of group A buds, g A根干重 is the average dry weight of roots in group A, g B芽干重 is the average dry weight of group B buds, g B根干重 is the average dry weight of roots in group B.

[0089] IV. Determine the drought resistance of Cyperus oleifera at the sprout stage based on the drought resistance evaluation system and the evaluation indicators obtained.

[0090] The drought resistance evaluation system uses the set relative germination rate F as the first identification index, the set relative bud length Y and relative root length D as the second identification index, and the set storage material transport rate P as the third identification index.

[0091] Furthermore, if the identified Cyperus oleifera does not meet the set first identification index, it is a sensitive Cyperus oleifera; if the identified Cyperus oleifera meets the set first identification index but does not meet the set second identification index, it is a common Cyperus oleifera; if the identified Cyperus oleifera meets the set first and second identification indexes but does not meet the set third identification index, it is a relatively drought-resistant Cyperus oleifera; if the identified Cyperus oleifera meets the set first, second and third identification indexes, it is an excellent drought-resistant Cyperus oleifera.

[0092] Effect test

[0093] Test 1. Disinfection test and result analysis:

[0094] The tubers of Cyperus oleiferus were disinfected by the methods of Examples 1 to 3 of the present invention respectively as Experimental Examples 1 to 3; 70% ethanol was used for disinfection: the Cyperus oleiferus tubers were rinsed with tap water for 3 times, each time for 5 minutes, and 70% ethanol was used for 60 seconds as Comparative Example 1; 6% NaClO was used for disinfection: the Cyperus oleiferus tubers were rinsed with tap water for 3 times, each time for 5 minutes, and 6% NaClO was used for 10 minutes as Comparative Example 2; the Cyperus oleiferus tubers were placed in an appropriate amount of 1 mg / L chlorine dioxide disinfectant at a solid-liquid ratio of 0.02 g / mL, and soaked for 60 minutes at 32°C, that is, only chlorine dioxide disinfectant was used for disinfection as Comparative Example 3, and then the disinfection effects of the Cyperus oleiferus tubers in each group were tested respectively. The relevant data are recorded in Table 1.

[0095] Table 1: Test data record table

[0096]

[0097] From Table 1 and Figure 1 It can be seen that the methods of Examples 1 to 3 can effectively disinfect the tubers of Cyperus oleifera, reduce the interference in the subsequent drought resistance identification of Cyperus oleifera during the sprouting period, and improve the accuracy of drought resistance identification.

[0098] This embodiment sets up the tests of Comparative Examples 1 and 2.

[0099] The main difference between the disinfection process of the comparative group 1 and the example group 1 is that 70% ethanol is used for disinfection. Compared with the example group 1, it is found that the germination rate of the comparative group 1 is reduced by 14%. This result shows that the disinfection effect of 70% ethanol disinfection on the tubers of cyperus oleifera is not as good as that of the example group 1.

[0100] The main difference between the disinfection process of the comparison group 2 and the example group 1 is that 6% NaClO is used for disinfection. Compared with the example group 1, the germination rate of the comparison group 2 was reduced by 34%. This result shows that the disinfection effect of 6% NaClO disinfection on the tubers of Cyperus oleifera is not as good as that of the example group 1.

[0101] The main difference between the disinfection process of the comparison group 3 and the example group 1 is that only chlorine dioxide disinfectant is used for disinfection. Compared with the example group 1, it is found that the germination rate of the comparison group 3 is reduced by 9%. This result shows that the disinfection effect of only using chlorine dioxide disinfectant on the tubers of cyperus oleifera is not as good as that of the example group 1.

[0102] Test 2: Drought resistance test and result analysis:

[0103] Five types of Cyperus oleifera with known drought resistance were selected: Cyperus oleifera HBBD> Cyperus oleifera SHGB> Cyperus oleifera XJTP> Cyperus oleifera YSD (100 of each). The identification was performed according to the method of Example 1, and the relevant data were recorded in Table 2, wherein the excellent drought-resistant Cyperus oleifera was A, the relatively drought-resistant Cyperus oleifera was B, the common Cyperus oleifera was C, and the sensitive Cyperus oleifera was D.

[0104] Table 2: Test data record table

[0105]

[0106] From Table 1 and Figure 2 It can be seen that the identification method provided by the present invention has excellent accuracy and can achieve rapid identification results.

[0107] From the above, it can be seen that the method for identifying drought resistance of Cyperus oleifera at the budding stage provided by the present invention can not only effectively reduce the risk of virus infection during disinfection of Cyperus oleifera, but also improve the germination rate of Cyperus oleifera; and it also has a high identification accuracy and can achieve a rapid identification effect. This shows that the method for identifying drought resistance of Cyperus oleifera at the budding stage provided by the present invention has a broader market prospect and is more suitable for promotion.

[0108] In the description of this specification, the description with reference to the terms "one embodiment", "example", "specific example", etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.

[0109] The preferred embodiments of the present invention disclosed above are only used to help illustrate the present invention. The preferred embodiments do not describe all the details in detail, nor do they limit the invention to the specific implementation methods described. Obviously, many modifications and changes can be made according to the content of this specification. This specification selects and specifically describes these embodiments in order to better explain the principles and practical applications of the present invention, so that those skilled in the art can understand and use the present invention well. The present invention is limited only by the claims and their full scope and equivalents.

Claims

1. A method for identifying drought resistance of Cyperus oleifera at the sprout stage, characterized in that: The following steps are involved: Ⅰ. Select the cyperus rotundus to be identified and disinfect it; The disinfection process is as follows: Place the tubers of Cyperus oleifera in an appropriate amount of 0.5-1 mg / L chlorine dioxide disinfectant at a solid-liquid ratio of 0.01-0.02 g / mL, and soak them at 30-32°C for 5-7 minutes; The soaked cyperus tubers are placed in a sealed container containing chlorine dioxide gas at a concentration of 0.012 to 0.015 g / mL and treated for 60 to 90 minutes at an air pressure of 40 to 60 kPa; Ⅱ. The sterilized Cyperus oleifera tubers were divided into Group A and Group B. Group A was planted in arid soil, and Group B was planted in soil with normal moisture content. The characteristics of Cyperus oleifera during germination were recorded. III. Calculate the evaluation index based on the recorded characteristics of germination period of Cyperus oleifera; IV. Identify the drought resistance of Cyperus oleifera at the sprout stage based on the drought resistance evaluation system and the obtained evaluation indicators; The traits of the germination period of the jatropha in step II include germination rate, bud length and root length; The evaluation indexes in step III include relative germination rate, relative bud length, relative root length and storage material transport rate, wherein: The calculation formula of the relative germination rate F is: The calculation formula of the relative bud length Y is: Y=y A组 -y B组 , where y A组 ,y B组 is the average of the bud lengths of group A and group B; The calculation formula of the relative root length D is: D = d A组 -d B组 , where d A组 ,d B组 is the average root length of group A and the average root length of group B; The calculation formula of the storage material transport rate P is: In the formula, g A芽干重 is the average dry weight of group A buds, g A根干重 is the average dry weight of roots in group A, g B芽干重 is the average dry weight of group B buds, g B根干重 is the average dry weight of roots in group B; The drought resistance evaluation system in step IV is an evaluation system with the set relative germination rate F as the first identification index, the set relative bud length Y and relative root length D as the second identification index, and the set storage material transport rate P as the third identification index; In step IV, if the identified Cyperus oleifera does not meet the set first identification index, it is a sensitive Cyperus oleifera; if the identified Cyperus oleifera meets the set first identification index but does not meet the set second identification index, it is a common Cyperus oleifera; if the identified Cyperus oleifera meets the set first identification index and the second identification index but does not meet the set third identification index, it is a relatively drought-resistant Cyperus oleifera; if the identified Cyperus oleifera meets the set first identification index, the second identification index and the third identification index, it is an excellent drought-resistant Cyperus oleifera.

2. The method for identifying drought resistance of Cyperus oleifera at sprout stage according to claim 1, characterized in that: The cyperus juncea tubers to be identified in step I are plump and uniformly sized cyperus juncea tubers.

3. The method for identifying drought resistance of Cyperus oleifera at sprouting stage according to claim 1, characterized in that: The moisture content of the dry soil in Group A in step II is selected to be 5% to 10%.

4. The method for identifying drought resistance of Cyperus oleifera at the sprouting stage according to claim 1, wherein: The moisture content of the normal moisture content soil of group B in step II is selected to be 40% to 70%.

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