An attractant composition for citrus psyllid adults and its application

Abstract

The present invention discloses an attractant composition for adult citrus psyllids and its use. The composition comprises the following components by weight: 0.1-1.1 parts of α-phellandrene, 0.1-0.8 parts of 1-butyl-2-isobutyl phthalate, 4.6-5.6 parts of trans-nerolidol, and 3.5-4.5 parts of β-caryophyllene. The synergist provided by the present invention is inexpensive, can reduce the cost of producing the attractant core, and is suitable for widespread promotion.

Description

Technical field:

[0001] The invention belongs to the technical field of pest monitoring and trapping, and particularly relates to an attractant composition for citrus psyllid adults and an application thereof. Background technology:

[0002] The citrus psyllid, Diaphorina citri, is a major pest of citrus during the new shoot phase and a vector of citrus Huanglongbing (Huanglongbing) disease. The psyllid feeds and lays eggs on infected citrus plants, producing large numbers of infected adults. These adults then move to infest new plants, spreading the disease. Citrus Huanglongbing is the most devastating disease in citrus production and is a recognized global crop protection challenge and disaster. Currently, there are no commercially grown citrus varieties resistant or tolerant to the disease, nor are there any specific drugs for controlling Huanglongbing. Controlling citrus Huanglongbing requires eliminating the vector insects that transmit the disease. Current control methods for citrus psyllids still primarily rely on chemical pesticides. However, chemical pesticides are highly toxic and require high dosages, causing environmental pollution. Furthermore, applying pesticides during the shoot phase of citrus orchards significantly increases labor and technical costs. Green control using ecological methods can reduce the use of chemical pesticides and protect human health.

[0003] Identifying compounds that attract adult citrus psyllids could be beneficial for preventing their oviposition and maintaining crop production. Currently, common ecological trapping techniques for Hemiptera pests include pheromones and color-based lures for adults and nymphs. However, these techniques are associated with high labor and material costs, poor stability, significant environmental waste generation, and difficulty in widespread adoption.

[0004] Ideal trapping has a significant impact on pest control. Attractants based on plant-derived volatiles satisfy pests' need for their preferred food, effectively concentrating them in a location directly affected by chemical agents. Attractants can be used to gather adult citrus psyllids, preventing them from concentrating their damage to crops during the peak phase, while also serving as monitoring and enhancing control effectiveness. Compared to color-coded traps and light-based lures, attractants are more selective and long-lasting, and are simple to operate and promote. However, pheromones are only effective against a single sex and cannot detect and control entire populations of mixed sexes. Furthermore, attractants can be used in conjunction with other agents or methods to achieve highly efficient, centralized pest control. In actual production, chemical control is often carried out after pests emerge, while attractants can preemptively trap and kill pests, reducing the time and number of pests that harm agricultural production.

[0005] Therefore, it is necessary to provide an attractant core containing a citrus psyllid adult attractant composition to alleviate the problems of high cost, difficult operation, and difficulty in popularization, and to provide diversified options for the monitoring and prevention and control technology of citrus psyllid adults. Summary of the invention:

[0006] The purpose of the present invention is to overcome the defects of the existing citrus psyllid trapping technology, which is unable to efficiently monitor and centrally control citrus psyllid adults, has high labor costs and is difficult to popularize, and to provide a food attractant composition for citrus psyllid adults and its application, so as to reduce labor cost investment, improve trapping activity, and achieve centralized killing, thereby contributing to double reduction and reducing field production costs.

[0007] The first object of the present invention is to provide an attractant composition for citrus psyllid adults, which comprises the following components in parts by weight: 0.1 to 1.1 parts of α-phellandrene, 0.1 to 0.8 parts of 1-butyl-2-isobutyl phthalate, 4.6 to 5.6 parts of trans-nerolidol, and 3.5 to 4.5 parts of β-caryophyllene.

[0008] Preferably, the attractant composition comprises the following components in parts by weight: 0.6-1.1 parts of α-phellandrene, 0.3-0.8 parts of 1-butyl-2-isobutyl phthalate, 5.1-5.6 parts of trans-nerolidol, and 4-4.5 parts of β-caryophyllene.

[0009] Further preferably, the attractant composition comprises the following components in parts by weight: 0.6 parts of α-phellandrene, 0.3 parts of 1-butyl-2-isobutyl phthalate, 5.1 parts of trans-nerolidol, and 4.0 parts of β-caryophyllene; or the attractant composition comprises the following components in parts by weight: 1.1 parts of α-phellandrene, 0.8 parts of 1-butyl-2-isobutyl phthalate, 5.6 parts of trans-nerolidol, and 4.5 parts of β-caryophyllene.

[0010] The second object of the present invention is to provide the use of the above-mentioned attractant composition for citrus psyllid adults in attracting, controlling and / or monitoring citrus psyllids.

[0011] Preferably, the application includes applying the attractant composition for citrus psyllid adults to a blank bait core to attract citrus psyllid adults; or applying the attractant composition for citrus psyllid adults to a blank bait core, and then using the bait core in combination with a chemical pesticide / biological pesticide.

[0012] The third object of the present invention is to provide a method for controlling and / or monitoring citrus psyllid adults, which comprises applying the above-mentioned attractant composition for citrus psyllid adults to the habitat of citrus psyllids to attract citrus psyllids.

[0013] Preferably, the dosage of the attractant composition for citrus psyllid adults is 0.6 to 10 mg / m2.

[0014] Compared with the prior art, the advantages of the present invention are:

[0015] (1) Simple and flexible usage. The attractant composition for citrus psyllid adults provided by the present invention can be used together with multiple ingredients according to a certain ratio for monitoring, or in combination with pesticides for centralized killing for prevention and control, which has greater autonomy.

[0016] (2) Compared with traditional color plates and other attractants for psyllids, the use of bait cores containing citrus psyllid attractants has a longer-lasting effect in the field, is more environmentally friendly, and can significantly reduce the manpower required for monitoring / control.

[0017] (3) The synergist component provided by the present invention is low in price, can reduce the cost of making the lure core, and is suitable for popularization and promotion. Specific implementation method:

[0018] The present invention will be further described in detail below in conjunction with specific embodiments. The examples given are only for illustrating the present invention, not for limiting the scope of the present invention.

[0019] Example 1

[0020] An indoor olfactory electrophysiological experiment of citrus psyllid attractant cores was conducted at Zhongkai University of Agriculture and Engineering from July 7 to 19, 2022.

[0021] The components of the attractant are: α-phellandrene, 1-butyl-2-isobutyl phthalate, trans-nerolidol and β-caryophyllene, and the purity of the components is above 99%.

[0022] Prepare 10 mg / mL solutions of α-phellandrene, 1-butyl-2-isobutyl phthalate, trans-nerolidol, and β-caryophyllene in n-hexane. Add 10 μL of each solution to a 1 cm² filter paper sheet. After the n-hexane evaporates, the attractant odor source is obtained. Place the resulting filter paper into a 15 cm long Pasteur tube, seal it with aluminum foil, and store at -20°C.

[0023] Mix the components listed in Table 1 and dissolve them in n-hexane to prepare a n-hexane solution of the multi-component synergist. The combined concentration of the solution is 10 mg / ml. Add 10 μl dropwise to a 1 cm² piece of filter paper. After the n-hexane evaporates, the multi-component attractant odor source is obtained. Place the resulting multi-component test filter paper into a 15 cm long Pasteur tube, seal it with aluminum foil, and store at -20°C.

[0024] Adult citrus psyllids of similar size were selected and starved in a box for two hours. Their heads were removed and connected to one of their antennae using conductive glue to a Y-shaped electrode produced by Syntech. This electrode was then connected to a Syntech IDAC2 potentioantennometer. Using a CS-55 air pump connected to a Pasteur tube containing an odor source, the odor was blown toward the antennae for 200 milliseconds, while the voltage (mV) generated by the antennae in response to the odor was recorded on the IDAC2. The experiment was conducted on both male and female insects, with six replicates for each component to be screened. The results are shown in Table 2.

[0025] In the experimental data in Table 2, groups AF to DM show the physiological activity of single-compound odor sources, with F representing female adults and M representing male adults; Solvent-F and Solvent-M are blank controls containing n-hexane without added odor components; and groups MF and MM show the effects of a self-prepared attractant composition (multi-component attractant core) for citrus psyllid adults. As can be seen from the table, after using the attractant composition (multi-component attractant core) containing the citrus psyllid adult attractant of this example, the electrophysiological activity of the single compound against citrus psyllid adults reached 0.124 to 0.429 millivolts, while the electrophysiological activity of the combination attractant core (multi-component attractant core) was even stronger, reaching 0.505 to 0.623 millivolts.

[0026] Table 1 Composition of multi-component attractants

[0027] Element Molecular weight Molecular formula Standard sample concentration, % parts by weight α-Pheretene 136 <![CDATA[C 10 H 16 ]]> 100 0.6 1-Butyl-2-isobutyl phthalate 278 <![CDATA[C 16 H 22 O4]]> 100 0.3 trans-nerolidol 222 <![CDATA[C 15 H 26 O]]> 100 5.1 β-Caryophyllene 204 <![CDATA[C 15 H 24 ]]> 100 4.0

[0028] Table 2 Electrophysiological test data

[0029]

[0030] Example 2

[0031] An indoor baiting test of citrus psyllid attractant cores was conducted at Zhongkai University of Agriculture and Engineering from August 1 to 31, 2022.

[0032] Prepare 10 mg / ml solutions of α-phellandrene, 1-butyl-2-isobutyl phthalate, trans-nerolidol, and β-caryophyllene using n-hexane as the solvent. Add 200 μl of each solution dropwise to the septum of a rubber stopper cleaned with n-hexane. After the n-hexane evaporates, the attractant core is prepared. Seal the core in aluminum foil and store at -20°C.

[0033] Mix 1.1 parts α-phellandrene, 0.8 parts 1-butyl-2-isobutyl phthalate, 5.6 parts trans-nerolidol, and 4.5 parts β-caryophyllene, by weight, and dissolve them in n-hexane to prepare a 10 mg / ml n-hexane solution of the multi-component attractant. Add 200 μL dropwise to the septum of a rubber stopper cleaned with n-hexane. After the n-hexane evaporates, the multi-component attractant core is prepared. Enclose the core in aluminum foil, seal it, and store at -20°C.

[0034] Adult citrus psyllids of similar size were selected and starved for two hours in a box. A single-component or combination lure (multi-component lure) was placed at a random position in a 20 cm diameter, four-arm olfactometer. A control lure, diluted with 200 μl of n-hexane, was placed on the other three sides. Groups of ten citrus psyllid adults were transferred to the center of the olfactometer, the lid was closed, and a CS-55 air pump was used to maintain air flow from the four outer odor sources to the central outlet of the instrument. The lure selection was recorded for 30 minutes. A new set of lures was replaced after every three groups of insects were tested. Citrus psyllids attracted to the attractant-side lure will move to the attractant-side lure. Three replicates were conducted for each component to be screened, totaling 30 adults. The attraction rate of the lure to citrus psyllid adults was calculated using the formula: ratio = number of adults that chose the odor source / (number of adults that chose the odor source + number of adults that did not choose the odor source + number of adults that chose the control solvent) × 100%. The results are shown in Table 3.

[0035] In the experimental data in Table 3, groups AF to DM show the effects of using single compound lures, where F represents female insects and M represents male insects; groups MF and MM show the effects of using a self-mixed attractant composition lure for adult citrus psyllids (multi-component attractant lure). As can be seen from the table, after using the attractant composition lure containing the adult citrus psyllid attractant of this example, the single compound attracted 12.9% to 41.7% of the adult citrus psyllids, while the attractant composition lure had an even higher attraction rate of 55.0% to 61.1%.

[0036] Table 3 Olfactometer test data

[0037]

[0038]

[0039] The above are merely preferred embodiments of the present invention. It should be noted that the above preferred embodiments should not be construed as limiting the present invention, and the scope of protection of the present invention should be determined by the scope defined in the claims. Persons skilled in the art will appreciate that improvements and modifications may be made without departing from the spirit and scope of the present invention, and such improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. An attractant composition for citrus psyllid adults, characterized in that: The attractant composition consists of the following components in parts by weight: 0.6-1.1 parts of alpha-phellandrene, 0.3-0.8 parts of 1-butyl-2-isobutyl phthalate, 5.1-5.6 parts of trans-nerolidol and 4-4.5 parts of beta-caryophyllene.

2. The attractant composition for citrus psyllid adults according to claim 1, characterized in that The attractant composition consists of the following components in parts by weight: 0.6 parts of α-phellandrene, 0.3 parts of 1-butyl-2-isobutyl phthalate, 5.1 parts of trans-nerolidol and 4.0 parts of β-caryophyllene.

3. The attractant composition for citrus psyllid adults according to claim 1, characterized in that The attractant composition consists of the following components in parts by weight: 1.1 parts of α-phellandrene, 0.8 parts of 1-butyl-2-isobutyl phthalate, 5.6 parts of trans-nerolidol and 4.5 parts of β-caryophyllene.

4. Use of the attractant composition for citrus psyllid adults according to claim 1, 2 or 3 in attracting, controlling and / or monitoring citrus psyllids.

5. The use according to claim 4, characterized in that The application is to apply the attractant composition for citrus psyllid adults to a blank bait core to attract citrus psyllid adults; or to apply the attractant composition for citrus psyllid adults to a blank bait core, and then use the bait core in combination with a chemical pesticide / biological pesticide.

6. A method for controlling and / or monitoring citrus psyllid adults, characterized in that: The attractant composition for adult citrus psyllids according to claim 1, 2 or 3 is applied to the habitat of citrus psyllids to attract citrus psyllids.

7. The method according to claim 6, characterized in that The dosage of the attractant composition for citrus psyllid adults is 0.6-10 mg / m2.

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