An acne-removing and inflammation-removing mask and a preparation method thereof

Abstract

The application discloses an acne-removing and inflammation-reducing facial mask and a preparation method thereof. The facial mask contains drug-loaded hyaluronic acid polypeptide nanoparticle emulsion, glycerol and nicotinamide. The drug-loaded hyaluronic acid polypeptide nanoparticle emulsion is an oil-in-water pickering emulsion with drug-loaded hyaluronic acid polypeptide nanoparticles as an emulsifier and an oil phase containing tea tree essential oil. The drug-loaded hyaluronic acid polypeptide nanoparticles are nanoparticles self-assembled from dopamine-modified hyaluronic acid and antibacterial peptides in a solution containing drugs. The nanoparticles have hyaluronidase responsiveness. When there are many acne pathogenic bacteria in the skin, the release of the content antibacterial peptides and cryptotanshinone can be accelerated, the stimulation of cryptotanshinone on the skin can be reduced, and a long-acting and slow-release effect can be achieved.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of skin care products, and particularly relates to a kind of acne-removing and inflammation-reducing facial mask and a preparation method thereof. BACKGROUND

[0002] Acne is a common chronic inflammatory disease of hair follicle and sebaceous gland caused by multiple factors, and its clinical manifestations include papules, pustules, nodules, cysts and scars. Current research suggests that the pathogenesis of acne is mainly related to the following four factors: excessive sebum secretion, hyperkeratosis of the opening of the sebaceous duct, Propionibacterium acnes (P. acnes) proliferation and inflammatory reaction. The etiology of acne is closely related to P. acnes. Modern research suggests that the growth of P. acnes mainly relies on triglyceride in sebum as nutrition, and obtains glycerol as energy, while the de-esterified fatty acid remains in the sebum, and its content is proportional to the number of bacteria. These free fatty acids can cause comedones, produce inflammatory stimuli and promote excessive secretion of sebaceous glands, leading to poor sebum excretion; at the same time, they promote the proliferation of P. acnes and other gram-positive bacteria such as Staphylococcus aureus and Staphylococcus epidermidis, leading to the production of inflammatory papules and pustules. The products used for treating acne in clinical practice mainly include topical retinoids, benzoyl peroxide and topical antibiotics, etc. Excessive use of these products may cause skin allergy, local erythema, desquamation, burning sensation and other irritating reactions, and cause secondary damage to the skin. At the same time, P. acnes has drug resistance and cross-resistance to many antibiotics, and antibiotics cannot completely eliminate P. acnes. With the continuous improvement of people's living standards, people pay more and more attention to the environmental protection, safety and naturalness of daily chemical products. Therefore, it is necessary to develop a natural and harmless acne-removing product. SUMMARY

[0003] In view of the problem that the acne-removing products in the prior art have great skin irritation and are easy to cause secondary damage to the skin, the purpose of the present application is to provide an acne-removing and inflammation-reducing facial mask, which is a leave-on and smear-type facial mask.

[0004] The purpose of the present application is achieved by adopting the following technical solutions:

[0005] An acne-removing and inflammation-reducing facial mask comprises the following components in parts by weight: drug-loaded hyaluronic acid polypeptide nanoparticle emulsion 10-30%, glycerol 5-10%, nicotinamide 0.3-0.5%, humectant 20-30%, preservative 1%, essence 0-0.05%, thickening agent 1-1.5%, pH adjuster 0-0.005%, and the balance is water.

[0006] The drug-loaded hyaluronic acid polypeptide nanoparticle emulsion is an oil-in-water pickering emulsion with drug-loaded hyaluronic acid polypeptide nanoparticles as emulsifier and oil phase containing tea tree essential oil.

[0007] Further, the oil phase further comprises an emollient, which includes one or a combination of dimethicone, cetyl stearyl alcohol, isopropyl myristate.

[0008] The drug-loaded hyaluronic acid polypeptide nanoparticle is used as a main active component of a mask and an emulsifier, and is a nanoparticle self-assembled from modified hyaluronic acid and polypeptides in a solution containing a drug.

[0009] Further, the modified hyaluronic acid is dopamine-modified sodium hyaluronate; the drug is cryptotanshinone; and the polypeptide is an antibacterial peptide.

[0010] Further, the antibacterial peptide is a pepsin hydrolysate of bovine lactoferrin.

[0011] In the present application, the modified hyaluronic acid, the antibacterial peptide and the cryptotanshinone are combined to obtain nanoparticles under the action of various non-covalent forces (π-π stacking interaction, hydrophilic and hydrophobic interaction, hydrogen bonding, electrostatic interaction), which can be used as a pickering emulsifier in a mask component to encapsulate tea tree oil to obtain an oil-in-water emulsion.

[0012] Further, cryptotanshinone is the component with the highest antibacterial activity in the liposoluble components of Salvia miltiorrhiza Bunge, and has strong activity in improving microcirculation, subsiding inflammation and repairing skin lesions; hyaluronic acid (sodium salt) is a natural moisturizing factor, which can promote the transdermal absorption of other nutrients while moisturizing; the antibacterial peptide is a cationic amphiphilic polypeptide with less than 50 amino acids, which has good biocompatibility and broad-spectrum antibacterial activity on different bacteria (gram-negative bacteria / positive bacteria, fungi) according to the sequence and conformation; the glycerol is used as a moisturizing agent to reduce the production of sebum and avoid the metabolism of triglycerides to free fatty acids by bacteria (glycerol as energy), thereby reducing inflammatory stimulation; nicotinamide is a vitamin with strong anti-inflammatory properties; tea tree oil has the effects of sterilization and inflammation reduction, pore contraction and treatment of suppurative wounds, and can bring a cooling feeling to the skin, but it has strong keratin layer corrosion and can cause dryness and desquamation of the skin during use.

[0013] The pathogenic bacteria of skin acne are mainly propionibacterium acnes and staphylococcus aureus, and the two bacteria can secrete bacterial hyaluronidase, which can finally degrade hyaluronic acid into unsaturated disaccharides as carbon source nutrients, and the pathogenic bacteria degrade the hyaluronic acid in the host body by using the produced hyaluronidase, destroy the defense system, reduce the viscosity, and facilitate the invasion and reach the ideal site. The presence of modified hyaluronic acid, on the one hand, produces a competitive effect, reduces the degradation of the source hyaluronic acid; on the other hand, the content of the modified hyaluronic acid hydrolysis end product (disaccharide) is reduced, and the bacterial growth is inhibited; and on the other hand, the number of bacteria becomes the flow valve of drug release, when the bacteria are more, the more hyaluronidase is secreted, the faster the nanometer particle degrades, and the more the active ingredients of antibacterial peptide, cryptotanshinone and tea tree oil are released, which greatly reduces the irritation of tanshinone and tea tree oil to the skin, and prolongs the action time.

[0014] Further, the thickening agent is selected from one or a combination of carbomer, xanthan gum and hydroxyethyl cellulose;

[0015] The pH regulator is selected from citric acid or triethanolamine;

[0016] The preservative is selected from one or a combination of methylparaben, phenoxyethanol and chlorphenesin;

[0017] The humectant is selected from one or a combination of propylene glycol, butylene glycol and sorbitol;

[0018] The application provides a preparation method of the drug-loaded hyaluronic acid polypeptide nanoparticle, comprising the following steps:

[0019] (1) Preparation of antibacterial peptide solution

[0020] A 40mg / mL lactoferrin aqueous solution is prepared, warmed to 37℃, and after 5min of incubation, 1M HCl solution is added to adjust the pH to 2, and pepsin is added in an amount of [E] / [S]=3%, and after 4h of reaction at a stable temperature and pH, the temperature is raised to 80℃ to inactivate the enzyme, 1M NaOH solution is added to adjust the solution to neutral, and the supernatant is freeze-dried to obtain a freeze-dried powder for standby.

[0021] (2) Preparation of modified hyaluronic acid

[0022] In an ice bath, sodium hyaluronate is reacted with dopamine under the catalysis of EDC·HCl / NHS to prepare modified hyaluronic acid.

[0023] The viscosity-average molecular weight of the sodium hyaluronate is 10 5 ~10 6 Da; and the grafting rate of the dopamine is 25~35%.

[0024] (3) Preparation of drug-loaded hyaluronic acid polypeptide nanoparticles

[0025] A DMF solution of modified sodium hyaluronate is prepared, and then cryptotanshinone is dissolved in the solution. After uniform stirring, a polypeptide aqueous solution is added dropwise, and stirring is performed overnight. The solution is transferred into a dialysis bag for dialysis, and finally, deionized water is added to obtain a drug-loaded hyaluronic acid polypeptide nanoparticle aqueous dispersion.

[0026] The concentration of the DMF solution of modified sodium hyaluronate is 10 mg / mL, the final concentration of cryptotanshinone is 0.2-0.4 mg / mL, and the concentration of the polypeptide aqueous solution is 0.5-1 mg / mL, and the mixing ratio of the two is 1:4 (v / v).

[0027] The application also provides a preparation method of the above-mentioned acne-removing and inflammation-reducing mask, which comprises the following steps:

[0028] (1) An oil phase is obtained by mixing an emollient and tea tree oil, and an aqueous phase is obtained by using the drug-loaded hyaluronic acid polypeptide nanoparticle aqueous dispersion. After mixing the two phases according to a certain volume, an oil-in-water emulsion is obtained by homogenization at a rotation speed of 8000 rpm at room temperature.

[0029] (2) Nicotinamide, a humectant, glycerol, a preservative, a thickening agent and water are mixed uniformly at 80℃, and then cooled for standby.

[0030] (3) The solutions obtained in steps (1) and (2) are mixed and stirred uniformly at 45℃.

[0031] (4) Fragrance and a pH adjuster are added to the solution obtained in step (3), and the pH is adjusted to 5-6.

[0032] Beneficial effects:

[0033] (1) The acne-removing and inflammation-reducing mask provided by the application contains drug-loaded hyaluronic acid polypeptide nanoparticles. On the one hand, the nanoparticles have hyaluronidase responsiveness, and when there are many acne-causing bacteria in the skin, the release of the content antimicrobial peptide and cryptotanshinone can be accelerated, which can reduce the irritation of cryptotanshinone to the skin and play a long-acting and slow-release role. On the other hand, the nanoparticles also act as a pickering emulsifier for the emollient and tea tree oil, which has a slow-release effect on the tea tree oil, reduces the irritation of the tea tree oil to the skin, and maintains the stability of each component to prevent sedimentation.

[0034] (2) The acne-removing and inflammation-reducing mask provided by the application has excellent antibacterial and anti-inflammatory activity. On the one hand, it benefits from the high activity of the antimicrobial peptide. Experiments have shown that the activity of the antimicrobial peptide can be greatly improved after the combination of dopamine-modified sodium hyaluronate and the antimicrobial peptide. On the other hand, it benefits from the synergistic effect of cryptotanshinone and tea tree oil. BRIEF DESCRIPTION OF DRAWINGS

[0035] Figure 1Preparation of modified sodium hyaluronate 1 H-NMR spectrum.

[0036] Figure 2 Optical microscope photos of emulsion digital photos and droplets of Examples 8-10.

[0037] Figure 3 Time-dependent release of cryptotanshinone from the enzyme solution of different concentrations in Example 8. DETAILED DESCRIPTION

[0038] The application will be further described in conjunction with specific examples, but the examples do not limit the application in any form. Unless otherwise specified, the reagents, methods and equipment used in the application are conventional reagents, methods and equipment in the technical field.

[0039] Example 1

[0040] Preparation of antibacterial peptide solution.

[0041] Prepare a 40 mg / mL aqueous solution of lactoferrin, heat to 37℃, and after 5 min of incubation, add 1M HCl solution to adjust the pH to 2. Add pepsin at an [E] / [S] ratio of 3%, and after 4 h of reaction at a stable temperature and pH, heat to 80℃ to inactivate the enzyme. Add 1M NaOH solution to adjust the solution to neutral, freeze and centrifuge for 30 min, and freeze-dry the supernatant to obtain a lyophilized powder for use.

[0042] Lactoferrin is derived from bovine milk and purchased from Shanghai Yuanye Biotechnology Co., Ltd.

[0043] Pepsin (EC 3.4.23.1) is purchased from Sigma Company, USA.

[0044] Example 2

[0045] Preparation of modified hyaluronic acid.

[0046] Dissolve 5.05 g of sodium hyaluronate in 300 mL of 50 mmol / L sodium bicarbonate solution, and place in an ice bath at 0℃. After stirring and dissolving, add 2.4 g of EDC·HCl and 3 g of NHS, and stir until completely dissolved. Then add 1.17 g of dopamine, and stir under ice bath for 12 h. After the reaction is completed, the reaction solution is dialyzed against ionized water for 3 d, and then freeze-dried to obtain lyophilized modified hyaluronic acid.

[0047] The sodium hyaluronate has a viscosity-average molecular weight of 10 5 ~ 10 6 Da, purchased from Zhenjiang Dongyuan Biotechnology Co., Ltd.

[0048]

Test 1

[0049] Test solvent: CDCl3, see attached spectra Figure 1 .

[0050] The grafting rate of dopamine in the modified hyaluronic acid was calculated to be 31.2%.

[0051] Examples 3-7

[0052] Preparation of drug-loaded hyaluronic acid polypeptide nanoparticles

[0053] A DMF solution of 10 mg / mL modified sodium hyaluronate was prepared, and then used to dissolve cryptotanshinone to a final concentration of 0.2, 0.3, and 0.4 mg / mL, respectively. A polypeptide aqueous solution of different concentrations was prepared, i.e., the freeze-dried powder prepared in Example 1 was dissolved in sterilized water to a concentration of 0.5, 0.8, and 1 mg / mL, respectively. The antibacterial peptide aqueous solution was added dropwise to the cryptotanshinone / modified sodium hyaluronate DMF solution at a volume ratio of 4:1, stirred for 12 h, transferred to a dialysis bag for dialysis, and finally diluted to 10 mL with deionized water to obtain a water dispersion of drug-loaded hyaluronic acid polypeptide nanoparticles.

[0054] The nanoparticles of different formulations are listed in Table 1.

[0055] Table 1

[0056]

[0057]

[0058] Comparative Example 1

[0059] Preparation of sodium hyaluronate / cryptotanshinone / antibacterial peptide nanoparticles

[0060] A DMF solution of 10 mg / mL sodium hyaluronate was prepared, and then used to dissolve cryptotanshinone to a final concentration of 0.3 mg / mL. An antibacterial peptide aqueous solution of 0.8 mg / mL was added dropwise to the above solution at a volume ratio of 4:1, stirred for 12 h, transferred to a dialysis bag for dialysis, and finally diluted to 10 mL with deionized water to obtain sodium hyaluronate / cryptotanshinone / antibacterial peptide nanoparticles.

[0061] Comparative Example 2

[0062] Preparation of modified sodium hyaluronate / antibacterial peptide nanoparticles

[0063] A DMF solution of 10 mg / mL modified sodium hyaluronate and an antibacterial peptide aqueous solution of 0.8 mg / mL were prepared, and the latter was added dropwise to the former at a volume ratio of 4:1, stirred for 12 h, transferred to a dialysis bag for dialysis, and finally diluted to 10 mL with deionized water to obtain modified sodium hyaluronate / antibacterial peptide nanoparticles.

[0064] Comparative Example 3

[0065] Preparation of sodium hyaluronate / antibacterial peptide nanoparticles.

[0066] A DMF solution of 10 mg / mL sodium hyaluronate was prepared, and a 0.8 mg / mL aqueous solution of antibacterial peptide was added dropwise to the above solution at a volume ratio of 4:1, stirred for 12 h, transferred to a dialysis bag for dialysis, and finally diluted with deionized water to 10 mL to obtain sodium hyaluronate / antibacterial peptide nanoparticles.

[0067]

Test 2

[0068] The particle size and Zeta potential of the nanoparticles were tested using a BI-90Plus Zeta potential shift produced by Brookhaven Instruments Corporation, USA, under the following conditions: room temperature and a scattering angle of 90°.

[0069] The test results of Examples 3-7 and Comparative Examples 1-3 are shown in Table 2.

[0070] Table 2

[0071] Sample Particle size, nm Z potential, mV Antibacterial peptide solution, pH = 7 - 12.28 Modified polydopamine solution, pH = 7 - -36.33 Example 3 316 -19.65 Example 4 327 -20.62 Example 5 324 -20.91 Example 6 362 -28.54 Example 7 346 -15.27 Comparative Example 1 372 -26.38 Comparative Example 2 338 -19.02 Comparative Example 3 365 -26.71

[0072]

Test 3

[0073] A gel-breaking solution was prepared: a methanol solution with a pH of 7-8, prepared by dissolving NaOH solid in methanol.

[0074] 1 mL of the nanoparticle dispersion was taken and placed in a 10 mL volumetric flask, and the gel-breaking solution was added to make up the volume, and ultrasonicated for 10 min and shaken well. 1 mL was accurately measured and centrifuged at 14000 rpm and 50°C for 10 min, and the supernatant was taken and placed in a 5 mL volumetric flask, and methanol was added to dissolve and make up to the mark. The content of cryptotanshinone was determined by high performance liquid chromatography, and converted to the content of cryptotanshinone in 1 mL of the nanoparticle dispersion. 1 mL of the nanoparticle dispersion was freeze-dried to obtain the total mass of the nanoparticles. The drug loading was calculated by the following formula: drug loading = content of cryptotanshinone in nanoparticles / total mass of nanoparticles.

[0075] The test results are shown in Table 3.

[0076] Table 3

[0077] Sample Drug loading, mg / g Example 3 17.30 Example 4 24.09 Example 5 31.78 Example 6 26.87 Example 7 25.27 Comparative Example 1 18.78

[0078] Preparation of pickering emulsion of tea tree oil.

[0079] The tea tree essential oil was mixed with polydimethylsiloxane so that the concentration of the tea tree essential oil was 50 wt%, as the oil phase, and a nanoparticle dispersion liquid was used as the water phase, and the volume ratio of the mixture was 1:2. After mixing, the mixture was homogenized at 8000 rpm for 5 min to obtain a tea tree essential oil pickering emulsion.

[0080] Table 4

[0081] Pickering emulsion Nanoparticle Example 8 Example 4 Example 9 Example 6 Example 10 Example 7 Comparative Example 4 Comparative Example 1 Comparative Example 5 Comparative Example 2 Comparative Example 6 Comparative Example 3

[0082] The tea tree essential oil was purchased from Shanghai Yuanye Biotechnology Co., Ltd., and the polydimethylsiloxane was purchased from Wacker Chemical (China) Co., Ltd.

[0083]

Test 4

[0084] A digital camera was used to take pictures of the apparent state of the emulsion when it was placed flat, and the emulsion rate = emulsion layer volume / total liquid volume was calculated to characterize the stability of the emulsion.

[0085] An optical microscope was used to observe the morphology of the emulsion droplets, and the particle size of the droplets was counted.

[0086] The data results are listed in Table 5, and the photos are listed in Figure 2 .

[0087] Table 5

[0088] Sample Emulsification rate, % Particle size, pm Example 8 93.5 40 Example 9 87.3 46 Example 10 94.3 38

[0089]

Test 5

[0090] The microbial source of hyaluronidase was produced by Nanjing Detai Biological Engineering Co., Ltd. according to the hyaluronidase gene sequence (GenBank Accession No. KF906414.1) published by NCBI, with E. coli (BL21) as the engineering bacteria, and the enzyme activity was 4722 U / mg.

[0091] The pickering emulsion described above was mixed with a hyaluronidase buffer solution (PBS buffer, pH = 6) with different concentrations, and the volume ratio of the mixture was 1:1, and the final concentration of the hyaluronidase buffer solution was 0, 2, 5, 10, 20 μg / L. Then it was sealed in a dialysis bag and dialyzed in a PBS buffer at 37°C and pH = 6 for a certain period of time. 0.2 mL of the outer dialysate was taken and the content of cryptotanshinone therein was detected by high performance liquid chromatography.

[0092] Examples 8-10 were dialyzed in enzyme solutions with different concentrations for 2 h, and the content of cryptotanshinone is listed in Table 6.

[0093] Table 6 (unit μg / mL)

[0094] Enzyme concentration Example 8 Example 9 Example 10 0 0.2160 0.5007 0.2262 2 1.1580 1.7484 1.0763 5 1.8609 2.3815 1.8359 10 2.0760 2.9533 2.3434 20 2.3662 3.3521 2.7171

[0095] The release of cryptotanshinone in different concentrations of enzyme solution over time is shown in Figure 8. Figure 3 .

[0096]

Test 6

[0097] The antibacterial activity was tested by the inhibition zone experiment, and the test bacteria were Propionibacterium acnes ATCC11827 and Staphylococcus aureus ATCC25923, which were purchased from the China Center for Type Culture Collection.

[0098] 0.1 mL of bacteria solution with a concentration of 10 8 CFU / mL was coated on the medium, and high-pressure sterilized round filter paper pieces (6 mm in diameter) were attached to the medium, and 20 μL of pickering emulsion or nanoparticle dispersion (diluted by one volume) was added. A blank control group was set up, and the antibacterial peptide prepared in Example 1 was used as a positive control group (concentration of 0.16 g / L). The samples were incubated in a 37°C constant temperature incubator for 10 h, and the diameter of the inhibition zone was measured. The experiment was repeated 3 times, and the average value was taken.

[0099] The results of the antibacterial activity test are shown in Table 7.

[0100] Table 7 (unit: mm)

[0101] Sample P. acnes S. aureus Example 4 26.64 18.33 Example 6 23.87 17.01 Example 7 28.99 18.83 Example 8 31.54 20.18 Comparative Example 2 19.43 12.96 Comparative Example 5 22.36 15.65 Antibacterial peptide lyophilized powder solution 24.25 18.05

[0102] A facial mask for acne and inflammation was prepared.

[0103] An acne and inflammation facial mask, by mass percentage, comprises the following components, and the formulations are listed in Table 8.

[0104] Table 8

[0105]

[0106]

[0107] Carbomer (Lubrizol), xanthan gum, and hydroxyethyl cellulose were purchased from Beijing Xiyake Business and Trade Co., Ltd.

[0108]

Test 7

[0109] The subjects are adult patients with I-III grade acne, aged 25-50 years, each sample and each grade of patients is a group, and each group contains 20 people; the specific test method is as follows: after the subjects wash their faces with warm water, the mask of the application is applied (5g±0.2 each time), and after 8 hours, the mask is washed off, and the treatment is repeated 3 times a week, and after 30 days of use, the therapeutic effect is counted.

[0110] The therapeutic effect is evaluated by using Pillsburg four-stage modified grading method:

[0111] Grade I (mild), the main skin lesions are blackhead acne, and the inflammatory papules are scattered or multiple.

[0112] The total number of lesions is 10-30;

[0113] Grade II (moderate), the main skin lesions are acne, and there are moderate number of papules and latent pustules, the total number of lesions is 31-50, and the lesions are localized on the face;

[0114] Grade III (moderate), the main skin lesions are deep inflammatory papules and pustules, the total number of lesions is 51-100, and the number of nodules is less than 3, and the lesions occur on the face, neck, chest and back;

[0115] Grade IV (severe), the main skin lesions are deep inflammatory papules and pustules, the total number of lesions is more than 100, and the number of nodules or cysts is more than 3, and the lesions are prone to form scars, and the lesions occur on the upper body.

[0116] The therapeutic effect is evaluated by using Pillsburg four-stage modified grading method:

[0117] Table 9

[0118]

[0119] The above examples are the preferred embodiments of the application, but the embodiments of the application are not limited to the above examples, and any changes, modifications, substitutions, combinations, simplifications made without departing from the spirit and principles of the application are equivalent replacement methods, and are all included in the protection scope of the application.

Claims

1. An acne-removing and anti-inflammatory facial mask, characterized in that: The facial mask comprises the following components: a drug-loaded hyaluronic acid polypeptide nanoparticle emulsion, glycerin, niacinamide, a moisturizer, a thickener, a pH regulator, and water; the drug-loaded hyaluronic acid polypeptide nanoparticle emulsion comprises drug-loaded hyaluronic acid polypeptide nanoparticles as an emulsifier, and the oil phase is an oil-in-water pickering emulsion containing tea tree essential oil; the drug-loaded hyaluronic acid polypeptide nanoparticles are nanoparticles self-assembled by dopamine-modified hyaluronic acid and antimicrobial peptides in a solution containing the drug; The antimicrobial peptide is a pepsin hydrolyzate of bovine lactoferrin; The preparation of the dopamine-modified hyaluronic acid comprises: in an ice bath, sodium hyaluronate reacts with dopamine under the catalysis of EDC·HCl and NHS to prepare the dopamine-modified hyaluronic acid; The preparation of the drug-loaded hyaluronic acid polypeptide nanoparticles comprises: preparing a DMF solution of dopamine-modified sodium hyaluronate, then dissolving cryptotanshinone in the solution to obtain a hyaluronic acid / cryptotanshinone solution, stirring evenly, dropwise adding an antimicrobial peptide aqueous solution, stirring overnight, transferring the solution to a dialysis bag for dialysis, and finally fixing the volume with deionized water to obtain an aqueous dispersion of the drug-loaded hyaluronic acid polypeptide nanoparticles. The concentration of the dopamine-modified sodium hyaluronate DMF solution is 10 mg / mL, the final concentration of cryptotanshinone in the hyaluronic acid / cryptotanshinone solution is 0.2-0.4 mg / mL, the concentration of the antimicrobial peptide aqueous solution is 0.5-1 mg / mL, and the mixing ratio of the hyaluronic acid / cryptotanshinone solution to the aqueous solution is 1:4 (v / v).

2. The acne-removing and anti-inflammatory facial mask according to claim 1, characterized in that: The facial mask also includes a preservative, which is selected from one or a combination of methylparaben, phenoxyethanol, and chlorphenesin.

3. The anti-acne and anti-inflammatory facial mask according to claim 2, characterized in that: The facial mask comprises the following components, calculated by weight percentage: 10-30% of drug-loaded hyaluronic acid polypeptide nanoparticle emulsion, 5-10% of glycerin, 0.3-0.5% of niacinamide, 20-30% of moisturizer, 1% of preservative, 1-1.5% of thickener, 0-0.005% of pH regulator, and the balance is water.

4. The anti-acne and anti-inflammatory facial mask according to claim 3, characterized in that: The oil phase also includes an emollient, which is selected from one or a combination of polydimethylsiloxane, cetearyl alcohol, and isopropyl myristate. The mass concentration of the tea tree essential oil in the oil phase is less than 50%.

5. The anti-acne and anti-inflammatory facial mask according to claim 3, characterized in that: The thickener is selected from one or a combination of carbomer, xanthan gum, and hydroxyethyl cellulose.

6. The anti-acne and anti-inflammatory facial mask according to claim 3, characterized in that: The moisturizing agent is selected from one or a combination of propylene glycol, butylene glycol and sorbitol.

7. The acne-removing and anti-inflammatory facial mask according to claim 3, characterized in that: The method for preparing the drug-loaded hyaluronic acid polypeptide nanoparticles comprises the following steps: (1) Preparation of antimicrobial peptide solution A 40 mg / mL aqueous lactoferrin solution was prepared and heated to 37°C. After incubation for 5 minutes, a 1 M HCl solution was added to adjust the pH to 2. Pepsin was added at a ratio of [E] / [S] = 3%. The reaction was allowed to proceed at a stable temperature and pH for 4 hours. The solution was then heated to 80°C to inactivate the enzyme. A 1 M NaOH solution was added to adjust the solution to neutrality. The solution was refrigerated and centrifuged for 30 minutes. The supernatant was freeze-dried to obtain a lyophilized powder for later use. (2) Preparation of modified hyaluronic acid In an ice bath, sodium hyaluronate reacts with dopamine under the catalysis of EDC·HCl / NHS to prepare modified hyaluronic acid; (3) Preparation of drug-loaded hyaluronic acid polypeptide nanoparticles Prepare a DMF solution of modified sodium hyaluronate, then dissolve cryptotanshinone in the above solution, stir evenly, add the antimicrobial peptide aqueous solution dropwise, stir overnight, transfer to a dialysis bag for dialysis, and finally adjust the volume with deionized water to obtain a drug-loaded hyaluronic acid polypeptide nanoparticle aqueous dispersion.

8. The method for preparing an anti-acne and anti-inflammatory facial mask according to claim 3, characterized in that: The steps include: (1) Mixing emollient and tea tree essential oil to obtain an oil phase, and using drug-loaded hyaluronic acid polypeptide nanoparticles aqueous dispersion as the aqueous phase, the two are mixed in a certain volume, and then homogenized at a speed of 8000 rpm at room temperature to obtain an oil-in-water emulsion; (2) Mix niacinamide, moisturizer, glycerin, preservative, thickener and water at 80°C and cool for later use; (3) Mix the solutions of step (1) and step (2) and stir them evenly at 45°C; Add a pH regulator to the solution in step (3) to adjust the pH to 5-6.

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