Temperature cycling device and detection device

Abstract

The application provides a temperature cycling device and a detection device. The temperature cycling device comprises a temperature adjusting assembly for adjusting the temperature of a sample in a container, the temperature adjusting assembly comprising a first temperature adjusting member and a second temperature adjusting member, the first temperature adjusting member and the second temperature adjusting member jointly forming a containing channel for containing the container; the temperature adjusting assembly and the container are relatively movable in a first preset direction to change the position of the container in the containing channel, so that the temperature cycling device has a first state and a second state; when the temperature cycling device is in the first state, the first temperature adjusting member contacts the container to adjust the sample in the container to a first temperature; when the temperature cycling device is in the second state, the second temperature adjusting member contacts the container to adjust the sample in the container to a second temperature; wherein the first temperature and the second temperature are different, and the first preset direction is the relative direction of the first temperature adjusting member and the second temperature adjusting member. The temperature cycling device provided by the application can improve the detection efficiency of the sample.

Description

Technical Field

[0001] This application relates to the field of molecular diagnostic technology, specifically to a temperature cycling device and a detection device. Background Technology

[0002] In the field of molecular diagnostics, samples need to be repeatedly heated and cooled to amplify them, thus facilitating detection. However, the heating and cooling process in related technologies is relatively slow, leading to reduced detection efficiency. Summary of the Invention

[0003] This application provides a temperature cycling device and a detection device, wherein the temperature cycling device can improve the detection efficiency of samples.

[0004] In a first aspect, this application provides a temperature circulation device, comprising: a temperature regulating component for adjusting the temperature of a sample in a container, the temperature regulating component including a first temperature regulating element and a second temperature regulating element, the first temperature regulating element and the second temperature regulating element together forming a receiving channel for receiving the container; the temperature regulating component and the container can move relative to each other in a first preset direction to change the position of the container within the receiving channel, such that the temperature circulation device has a first state and a second state; when the temperature circulation device is in the first state, the first temperature regulating element contacts the container to adjust the sample in the container to a first temperature; when the temperature circulation device is in the second state, the second temperature regulating element contacts the container to adjust the sample in the container to a second temperature; wherein the first temperature and the second temperature are different, and the first preset direction is the relative direction of the first temperature regulating element and the second temperature regulating element.

[0005] The first temperature regulating element includes a plurality of first heat transfer parts, which are spaced apart along a second preset direction to form a first gap. The first gap forms part of the receiving channel. When the temperature circulation device is in the first state, the container is located in the first gap and in contact with the first heat transfer parts.

[0006] The second temperature regulating element includes a plurality of second heat transfer parts, which are spaced apart along a second preset direction to form a second gap. The second gap constitutes part of the receiving channel. When the temperature circulation device is in the second state, the container is located in the second gap and in contact with the second heat transfer parts.

[0007] The temperature control component further includes a first heat insulation element, which includes a plurality of first sub-heat insulation parts. The first sub-heat insulation parts are connected to the first heat transfer part and the second heat transfer part. The plurality of first sub-heat insulation parts are spaced apart along a second preset direction to form a first connecting channel. The first connecting channel forms part of the receiving channel and connects the first gap and the second gap. The first heat insulation element is used to reduce the heat transfer process between the first temperature control element and the second temperature control element.

[0008] The first gap and the second gap are positioned opposite each other and are interconnected.

[0009] The temperature control assembly further includes a first heating element for heating the first temperature control element, and the first heating element is connected to each of the first heat transfer parts.

[0010] Each of the first gaps, along a first preset direction, is used to accommodate one or more of the containers.

[0011] Two adjacent first heat transfer sections are used to contact the same container.

[0012] Secondly, this application also provides a detection device, which includes a container and the temperature circulation device described in the first aspect of this application. The temperature regulating component of the temperature circulation device is movable relative to the container so that the temperature circulation device has a first state and a second state. When the temperature circulation device is in the first state, the first temperature regulating element of the temperature regulating component contacts the container; when the temperature circulation device is in the second state, the second temperature regulating element of the temperature regulating component contacts the container.

[0013] When the temperature circulation device is in the first state, the opposite sides of the container are in contact with the first temperature regulating element; when the temperature circulation device is in the second state, the opposite sides of the container are in contact with the second temperature regulating element.

[0014] In this application, the container only comes into contact with one of the first and second temperature-regulating components during the temperature cycling process. Therefore, during operation, the first and second temperature-regulating components can respectively maintain the temperature at a first temperature and a second temperature. When it is necessary to raise the temperature of the sample in the container to the first temperature, it is only necessary to bring the container into contact with the first temperature-regulating component. When it is necessary to cool the sample in the container to the second temperature, it is only necessary to bring the container into contact with the second temperature-regulating component. Thus, to achieve temperature cycling between the first and second temperatures in the container, it is only necessary to maintain the temperatures of the first and second temperature-regulating components and to allow relative movement between the container and the temperature-regulating components, without changing the temperatures of the first and second temperature-regulating components themselves. It is understood that, compared to the method in related technologies that achieves temperature cycling by changing the temperature, the time required for relative movement is less than the time required for changing the temperature. Therefore, using the solution provided in this application allows the sample in the container to rise or fall to the required temperature more quickly, thereby reducing the temperature cycling time and ultimately improving the sample detection efficiency. Attached Figure Description

[0015] To more clearly illustrate the technical solutions of the embodiments of this application, the drawings used in the implementation will be briefly introduced below. Obviously, the drawings described below are some implementations of this application. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0016] Figure 1 This is a schematic diagram of a temperature control component provided in one embodiment of this application.

[0017] Figure 2 This is a schematic diagram of a container provided in one embodiment of this application.

[0018] Figure 3 A schematic diagram of the cooperation between the container and the temperature control component provided in an embodiment of this application (first state).

[0019] Figure 4 This is a schematic diagram (second state) showing the cooperation between a container and a temperature control component provided in one embodiment of this application.

[0020] Figure 5 This is a schematic diagram of a first temperature regulating element provided in an embodiment of this application.

[0021] Figure 6 This is a schematic diagram of a temperature control component provided in another embodiment of this application.

[0022] Figure 7 A schematic diagram of the cooperation between the container and the temperature control component provided in another embodiment of this application (first state).

[0023] Figure 8 for Figure 2The diagram shows the container in direction A.

[0024] Figure 9 for Figure 3 The structure shown is a cross-sectional view along line BB.

[0025] Figure 10 This is a schematic diagram of a temperature control component provided in another embodiment of this application.

[0026] Figure 11 This is a diagram showing the positional relationship between the temperature control component and the optical device provided in an embodiment of this application.

[0027] Figure 12 This is a schematic diagram showing that the container and temperature control assembly provided in one embodiment of this application are in a conical fit. Detailed Implementation

[0028] The technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, and not all embodiments. Based on the embodiments of this application, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of this application.

[0029] In this document, references to "embodiment" or "implementation" mean that a particular feature, structure, or characteristic described in connection with an embodiment or implementation may be included in at least one embodiment of this application. The appearance of this phrase in various places throughout the specification does not necessarily refer to the same embodiment, nor is it a separate or alternative embodiment mutually exclusive with other embodiments. It will be explicitly and implicitly understood by those skilled in the art that the embodiments described herein can be combined with other embodiments.

[0030] Please refer to Figures 1 to 4 This application provides a detection device 100, which includes a container 20 and a temperature circulation device 10 as described in any of the following embodiments. The temperature circulation device 10 is used to regulate the temperature of the container 20. The container 20 may also be referred to as a reaction tank, chip, etc.

[0031] Furthermore, the temperature regulating component 11 of the temperature circulation device 10 can move relative to the container 20, so that the temperature circulation device 10 has a first state and a second state. When the temperature circulation device 10 is in the first state (e.g. Figure 3 As shown), the first temperature regulating element 111 of the temperature regulating assembly 11 contacts the container 20 to adjust the sample in the container 20 to a first temperature. When the temperature circulation device 10 is in the second state (e.g. Figure 4As shown), the second temperature regulating element 112 of the temperature regulating assembly 11 contacts the container 20 to adjust the sample in the container 20 to a second temperature.

[0032] When the temperature circulation device 10 is in the first state, the opposite sides of the container 20 are in contact with the first temperature regulating element 111. When the temperature circulation device 10 is in the second state, the opposite sides of the container 20 are in contact with the second temperature regulating element 112. That is, both the first temperature regulating element 111 and the second temperature regulating element 112 can be in contact with the opposite sides of the container 20. This means that when heat transfer occurs between the container 20 and the temperature circulation device 10, at least two heat transfer paths are simultaneously conducting heat transfer, resulting in a greater heat exchange rate per unit time. Consequently, the sample in the container 20 can reach the first or second temperature more quickly.

[0033] The container 20 and the temperature circulation device 10 described above are described in detail below with reference to the accompanying drawings.

[0034] Please refer to Figures 1 to 4 This application provides a temperature circulation device 10, which includes a temperature regulating component 11 for adjusting the temperature of a sample in a container 20. The temperature circulation device 10 may further include a housing, with the temperature regulating component 11 disposed within the housing for protection. The temperature regulating component 11 includes a first temperature regulating element 111 and a second temperature regulating element 112, which together form a receiving channel D10 for receiving the container 20. The temperature regulating component 11 and the container 20 can move relative to each other in a first preset direction to change the position of the container 20 within the receiving channel D10, thus allowing the temperature circulation device 10 to have a first state and a second state. When the temperature circulation device 10 is in the first state (see...), ... Figure 3 The first temperature regulating element 111 contacts the container 20 to adjust the sample in the container 20 to a first temperature. When the temperature circulation device 10 is in the second state (see...), Figure 4 The second temperature regulating element 112 contacts the container 20 to adjust the sample in the container 20 to a second temperature. The first temperature and the second temperature are different. The first preset direction is the relative direction between the first temperature regulating element 111 and the second temperature regulating element 112, and it is also the extension direction of the receiving channel D10. Specifically, the relative direction between the first temperature regulating element 111 and the second temperature regulating element 112 refers to the direction from the first temperature regulating element 111 to the second temperature regulating element 112, and the direction from the second temperature regulating element 112 to the first temperature regulating element 111.

[0035] The following is a detailed description: When the temperature circulation device 10 is working, both the first temperature regulating element 111 and the second temperature regulating element 112 can be maintained at a constant temperature. Specifically, the first temperature regulating element 111 can be maintained at a first temperature, and the second temperature regulating element 112 can be maintained at a second temperature. The temperature regulating component 11 is used to adjust the temperature of the sample in the container 20, so that the sample in the container 20 circulates between the first temperature and the second temperature, thereby achieving sample amplification. The first temperature can be higher than or lower than the second temperature. For example, the first temperature can be 95°C, and the second temperature can be 60°C. The following embodiments of this application illustrate this with the first temperature being higher than the second temperature.

[0036] The first temperature regulating element 111 and the second temperature regulating element 112 are arranged at intervals. This arrangement is to avoid or weaken the heat transfer process between the two, thereby helping to ensure that they are maintained at the first and second temperatures respectively, thus making the temperature rise and fall of the container 20 accurate.

[0037] Container 20 is used to contain samples and liquid reagents. The samples may be, but are not limited to, hepatitis B virus samples, HIV samples, and COVID-19 samples. Container 20 can be a rigid container (such as a test tube) or a flexible container (such as a flexible membrane bag). A rigid container means that the shape of container 20 will not deform as reagents are filled in; conversely, a flexible container means that the shape of container 20 will deform as reagents are filled in. The following description uses a flexible container as an example.

[0038] When container 20 is filled with reagent, it expands due to the reagent, causing it to press tightly against temperature control component 11. This ensures full contact between the two components, improving heat transfer efficiency. The material of container 20 can be, but is not limited to, plastic. Optionally, when container 20 is expanded by the reagent, it forms a tight contact with temperature control component 11, reducing the gap between them and further improving heat transfer efficiency. This tight contact also allows container 20 to be held in place by temperature control component 11, preventing it from shaking and ensuring a continuous and efficient heat transfer process.

[0039] The temperature control component 11 is movable relative to the container 20, thereby switching between a first state and a second state, allowing the container 20 to contact either the first temperature control element 111 or the second temperature control element 112. In the first state, the container 20 contacts the first temperature control element 111, which transfers heat to the container 20, raising the sample temperature to the first temperature. When the container 20 contacts the second temperature control element 112, the second temperature control element 112 absorbs heat from the container 20, causing the sample temperature to drop from the first temperature to the second temperature, completing one cycle. One complete cycle results in one sample amplification. Therefore, as the container 20 repeatedly contacts the first and second temperature control elements 111 and 112, the temperature of the sample in the container 20 cycles between the first and second temperatures, achieving continuous sample amplification until the desired sample number is reached. Optionally, the total number of temperature cycles between the first and second temperatures is 40-45. Here, sample amplification refers to the process of increasing the sample quantity. Understandably, amplified samples make them easier to test in subsequent processes.

[0040] Furthermore, the first temperature regulating element 111 and the second temperature regulating element 112 together form a receiving channel D10, which is used to receive the container 20. It is understood that placing the container 20 within the receiving channel D10 restricts its movement, thereby preventing swaying during heat transfer. In addition, if the container 20 is a flexible container, its expansion can abut against the sidewall of the receiving channel D10, ensuring close contact between the container 20 and the temperature regulating component 11, thereby reducing thermal resistance and improving heat transfer efficiency.

[0041] Optionally, when the container 20 is placed in the receiving channel D10, the container 20 protrudes from the surface of the temperature control assembly 11, which makes it easier to remove the container 20 from the receiving channel D10.

[0042] In the first related technique, the sample is placed in a test tube, and then the test tube is repeatedly heated and cooled using a temperature control component. However, the test tube itself is relatively thick and has a high thermal resistance, resulting in low heat transfer efficiency. Furthermore, both the test tube and the temperature control component are made of rigid materials, and even when they are in contact, a large gap remains between them, further weakening the heat transfer process. From these two aspects, using test tubes to store samples leads to a slow heating and cooling process, ultimately resulting in low sample detection efficiency.

[0043] Compared to the first related technology, if the container 20 is a flexible container, when the container 20 is filled with reagents, regardless of the shape of the temperature control component 11, the container 20 can deform accordingly to conform to the shape of the temperature control component 11, thereby giving the container 20 and the temperature control component 11 a larger contact area, resulting in a larger heat transfer area. Simultaneously, the reagent-filled container 20 can more easily and tightly fit the temperature control component 11, reducing the gap between them, decreasing the thermal resistance, and increasing the heat transfer rate. This shortens the temperature rise and fall process of the sample within the container 20, ultimately improving the sample detection efficiency. Furthermore, the thickness of the container 20 can be designed to be thinner, which facilitates deformation and reduces thermal resistance, further shortening the temperature rise and fall process of the sample within the container 20.

[0044] In the second related technology, the container is in contact with the temperature control component, and the container and the temperature control component cannot move relative to each other. When it is necessary to heat the container to a first temperature, the temperature of the temperature control component is adjusted to the first temperature; when it is necessary to cool the container to a second temperature, the temperature of the temperature control component is adjusted to the second temperature. However, the temperature control component needs a certain amount of time to switch between the first and second temperatures, which prolongs the cycle time of the sample in the container between the first and second temperatures, thereby reducing the sample detection efficiency.

[0045] Compared to the second related technology, in this application, the container 20 only comes into contact with one of the first temperature regulating element 111 and the second temperature regulating element 112 during the temperature cycling process. Therefore, during operation, the first temperature regulating element 111 and the second temperature regulating element 112 can respectively maintain the temperature at a first temperature and a second temperature. When it is necessary to raise the temperature of the sample in the container 20 to the first temperature, it is only necessary to bring the container 20 into contact with the first temperature regulating element 111. When it is necessary to cool the sample in the container 20 to the second temperature, it is only necessary to bring the container 20 into contact with the second temperature regulating element 112. Thus, to achieve the cycling of the sample in the container 20 between the first temperature and the second temperature, it is only necessary to maintain the temperature of the first temperature regulating element 111 and the second temperature regulating element 112 and to allow relative movement between the container 20 and the temperature regulating assembly 11, without changing the temperature of the first temperature regulating element 111 and the second temperature regulating element 112 themselves. It is understandable that, compared to the second related technology, the time required for relative motion is less than the time required for temperature change. Therefore, using the solution provided in this application can make the sample in container 20 rise or fall to the required temperature more quickly, thereby reducing the temperature cycle time and ultimately improving the sample detection efficiency.

[0046] Please combine Figure 3 Reference Figure 5The first temperature regulating component 111 includes a first connecting portion 1111 and a plurality of first heat transfer portions 1112 connected to the first connecting portion 1111. The plurality of first heat transfer portions 1112 are spaced apart along a second preset direction to form a first gap D11, and the first gap D11 constitutes a portion of the receiving channel D10. When the temperature circulation device 10 is in the first state, the container 20 is located within the first gap D11 and in contact with the first heat transfer portions 1112, that is, the container 20 contacts at least one sidewall of the first gap D11.

[0047] Specifically, the first connecting portion 1111 is elongated, and multiple first heat transfer portions 1112 are arranged along the length of the first connecting portion 1111, with all first heat transfer portions 1112 connected to the same side of the first connecting portion 1111. The first connecting portion 1111 and the first heat transfer portions 1112 can be made of the same material or different materials. The shape and size of each first heat transfer portion 1112 can be the same or different. The shape of the first heat transfer portion 1112 can be, but is not limited to, a cube, cuboid, prism, etc. The number of first heat transfer portions 1112 can be, but is not limited to, 2, 3, 5, 7, 8, etc. It is understood that in this embodiment, the first heat transfer portions 1112, which are spaced apart from each other, are indirectly connected through the first connecting portion 1111, thereby ensuring that the temperature of each first heat transfer portion 1112 is the first temperature. In addition, each of the first heat transfer parts 1112 can be used to heat the container 20. Therefore, a first temperature regulating element 111 can simultaneously heat the samples in multiple containers 20 to the first temperature. In other words, the effect of the first temperature regulating element 111 on heating multiple containers 20 is consistent, which is beneficial to eliminate the influence of temperature on the samples during the test.

[0048] Furthermore, when container 20 is located in the first gap D11, container 20 contacts at least one of the first connecting part 1111 and the first heat transfer part 1112. Due to the contact relationship, heat can be transferred between the first temperature regulating element 111 and the sample in container 20, causing the sample to heat up to the first temperature. It can be understood that by placing container 20 within the first gap D11, the first connecting part 1111 and the first heat transfer part 1112 can restrict the degree of freedom of movement of container 20, thereby preventing container 20 from shaking and ensuring a good and stable heat transfer process between container 20 and the first temperature regulating element 111.

[0049] Furthermore, each first gap D11 can accommodate at least one container 20, and the specific number of containers 20 in each first gap D11 can be, but is not limited to, 1, 3, 4, 6, etc. It can be understood that N first heat transfer parts 1112 can constitute N-1 first gaps D11. If each first gap D11 can accommodate M containers 20, then one first temperature regulating element 111 can heat M*(N-1) containers 20.

[0050] Optionally, the first temperature regulating element 111 includes a plurality of first heat transfer parts 1112, which are spaced apart along a second preset direction to form a first gap D11, and the first gap D11 constitutes a portion of the receiving channel D10. When the temperature circulation device 10 is in the first state, the container 20 is located within the first gap D11 and in contact with the first heat transfer parts 1112. The difference between this embodiment and the above embodiment is that the first temperature regulating element 111 does not include a first connecting part 1111, but only has first heat transfer parts 1112.

[0051] Please combine Figure 4 Reference Figure 6 The second temperature regulating element 112 includes a second connecting portion 1121 and a plurality of second heat transfer portions 1122 connected to the second connecting portion 1121. The plurality of second heat transfer portions 1122 are spaced apart along a second preset direction to form a second gap D12, and the second gap D12 constitutes a portion of the receiving channel D10. When the temperature circulation device 10 is in the second state, the container 20 is located within the second gap D12 and in contact with the second heat transfer portions 1122, that is, the container 20 contacts at least one sidewall of the second gap D12.

[0052] Both the second gap D12 and the first gap D11 are approximately U-shaped, with their U-shaped openings facing each other. It should be noted that the second temperature regulating element 112 and the first temperature regulating element 111 may have the same shape and size, or they may be different. Details regarding the second temperature regulating element 112 can be found in the description of the first temperature regulating element 111 described above, and will not be repeated here.

[0053] Optionally, the second temperature regulating element 112 includes a plurality of second heat transfer parts 1122, which are spaced apart along a second preset direction to form a second gap D12, and the second gap D12 constitutes a portion of the receiving channel D10. When the temperature circulation device 10 is in the second state, the container 20 is located within the second gap D12 and in contact with the second heat transfer parts 1122. The difference between this embodiment and the above embodiment is that the second temperature regulating element 112 does not include a second connecting part 1121, but only has second heat transfer parts 1122.

[0054] Please refer to Figure 5 and Figure 6 The first gap D11 and the second gap D12 are positioned opposite each other and are interconnected. It is understood that interconnection means that the container 20 can directly enter the second gap D12 from the first gap D11, or enter the first gap D11 from the second gap D12. Positioning opposite each other means that the movement distance of the container 20 between the first gap D11 and the second gap D12 is minimized, thereby reducing movement time and improving detection efficiency. Optionally, the direction from the first gap D11 to the second gap D12 is a first preset direction, and the first preset direction and the second preset direction are perpendicular to each other.

[0055] Please combine Figure 5 Reference Figure 6 The temperature control assembly 11 further includes a first heating element 114 for heating the first temperature control member 111. The first heating element 114 is directly or indirectly connected to each of the first heat transfer parts 1112. In one embodiment, when the first temperature control member 111 includes a first connecting part 1111, the first heating element 114 can be connected to the first connecting part 1111, thereby indirectly connecting to the first heat transfer part 1112 through the first connecting part 1111. In another embodiment, when the first temperature control member 111 does not include the first connecting part 1111, the first heating element 114 can be directly connected to each of the first heat transfer parts 1112.

[0056] Optional, please refer to Figure 5 Reference Figure 6 The temperature control assembly 11 further includes a first heating element 114 for heating the first temperature control element 111. The first heating element 114 is connected to the first connecting portion 1111, and the first heating element 114 is at the same distance from each of the first heat transfer portions 1112.

[0057] Specifically, the first heating element 114 is used to transfer heat to the first temperature regulating element 111 to maintain the first temperature regulating element 111 at a first temperature. The first heating element 114 is generally elongated, and its extension direction is consistent with the extension direction of the first connecting portion 1111. The orthographic projection of each first heat transfer portion 1112 onto the first heating element 114 falls within the area of ​​the first heating element 114, thus ensuring that the distance from the first heating element 114 to each first heat transfer portion 1112 is the same. It is understood that since the first heating element 114 is directly connected to the first connecting portion 1111, the first heating element 114 needs to transfer heat to the first heat transfer portions 1112 through the first connecting portion 1111. If the distances from the first heating element 114 to each first heat transfer portion 1112 are inconsistent, it may lead to different temperatures in the different first heat transfer portions 1112, thereby affecting the detection effect. The arrangement in this embodiment avoids the problem of different temperatures.

[0058] Furthermore, the temperature control assembly 11 also includes a second heating element 115 for heating the second temperature control member 112. The second heating element 115 is directly or indirectly connected to each of the second heat transfer parts 1122. In one embodiment, when the second temperature control member 112 includes a second connecting part 1121, the second heating element 115 can be connected to the second connecting part 1121, thereby indirectly connecting to the second heat transfer part 1122 through the second connecting part 1121. In another embodiment, when the second temperature control member 112 does not include a second connecting part 1121, the second heating element 115 can be directly connected to each of the second heat transfer parts 1122.

[0059] Optionally, the second heating element 115 is connected to the second connecting portion 1121, and the distance from the second heating element 115 to each of the second heat transfer portions 1122 is the same. For a description of the second heating element 115, please refer to the accompanying drawings and description of the first heating element 114 in the above embodiments; further details will not be repeated here.

[0060] Please combine Figure 5 Reference Figure 6 The temperature regulating component 11 further includes a first heat insulation member 113, which is disposed between the first temperature regulating member 111 and the second temperature regulating member 112, and connected to the first heat transfer section 1112 and the second heat transfer section 1122. The first heat insulation member 113 is used to reduce the heat transfer process between the first temperature regulating member 111 and the second temperature regulating member 112.

[0061] It is understandable that, since the temperature of the first temperature regulating element 111 is greater than that of the second temperature regulating element 112, if the two are in direct contact or the equivalent thermal resistance between them is small, the first temperature regulating element 111 will definitely transfer heat to the second temperature regulating element 112 quickly, causing the temperature of the first temperature regulating element 111 to drop and the temperature of the second temperature regulating element 112 to rise, and the temperature difference between the two to become smaller and smaller, which will be detrimental to the amplification of the sample. In this embodiment, a first heat insulation member 113 is provided between the first temperature regulating member 111 and the second temperature regulating member 112, thereby increasing the thermal resistance between them. Increased thermal resistance means that heat transfer between the first and second temperature regulating members 111 and 112 is more difficult. In other words, the higher-temperature first temperature regulating member 111 is less likely to transfer heat to the lower-temperature second temperature regulating member 112. This allows the first and second temperature regulating members 111 and 112 to better maintain their respective temperatures, thus ensuring high temperature accuracy during the temperature change process of the sample in the container 20. This facilitates successful sample amplification and improves sample detection. Furthermore, the first heat insulation member 113 connects the first and second temperature regulating members 111 and 112, forming a single unit that facilitates installation, disassembly, and overall movement.

[0062] Furthermore, the first heat insulation member 113 is composed of a plurality of first sub-heat insulation parts 1131. Each first sub-heat insulation part 1131 is connected to the first heat transfer part 1112 and the second heat transfer part 1122. The plurality of first sub-heat insulation parts 1131 are spaced apart along a second preset direction to form a first connecting channel L1. The first connecting channel L1 constitutes part of the receiving channel D10, and the first connecting channel L1 connects the first gap D11 and the second gap D12. That is, every two adjacent first sub-heat insulation parts 1131 form a first connecting channel L1. The opposite ends of each first connecting channel L1 are respectively connected to the first gap D11 and the second gap D12. During the transition between the first state and the second state of the temperature circulation device 10, the container 20 will enter the first gap D11 or the second gap D12 through the first connecting channel L1.

[0063] Furthermore, the first heat insulation element 113 is made of a material with high thermal resistance. Optionally, the thermal resistance of both the first temperature regulating element 111 and the second temperature regulating element 112 is lower than that of the first heat insulation element 113. This configuration weakens the heat transfer process between the first temperature regulating element 111 and the first heat insulation element 113, as well as between the second temperature regulating element 112 and the first heat insulation element 113. This reduces the influence of the first heat insulation element 113 on the temperatures of the first temperature regulating element 111 and the second temperature regulating element 112, thus helping to maintain the first temperature regulating element 111 and the second temperature regulating element 112 at the first and second temperatures respectively. It also reduces heat loss, thereby saving electricity. Optionally, the thermal resistance of the first heat insulation element 113 is greater than that of the container 20. This configuration weakens the heat transfer between the first heat insulation element 113 and the container 20, thereby reducing the impact on the container 20 and improving the sample detection efficiency.

[0064] Furthermore, the first temperature regulating element 111 and the second temperature regulating element 112 can be made of materials with a high specific heat capacity. It is understood that a high specific heat capacity means that a large amount of heat needs to be gained or lost to cause a temperature change, which helps ensure that the first temperature regulating element 111 is maintained at a first temperature and the second temperature regulating element 112 is maintained at a second temperature; that is, the first temperature regulating element 111 and the second temperature regulating element 112 have heat preservation capabilities. Optionally, the specific heat capacity of the first temperature regulating element 111 and the second temperature regulating element 112 is greater than that of the first heat insulation element 113 and the container 20, thus reducing the influence of the first heat insulation element 113 and the container 20 on the temperature of the first temperature regulating element 111 and the second temperature regulating element 112. Taking the first temperature regulating element 111 as an example, if the specific heat capacity of the first temperature regulating element 111 is less than that of the first heat insulation element 113 and the container 20, the first temperature regulating element 111 may experience a temporary temperature drop due to the absorption of a small portion of heat by the first heat insulation element 113 and the container 20. That is, the temperature of the first temperature regulating element 111 may drop below the first temperature. Although it can eventually be heated back to the first temperature by the first heating element 114, the time it takes for the sample in the container 20 to reach the first temperature is prolonged, resulting in reduced detection efficiency. Therefore, the larger the specific heat capacity of the first temperature regulating element 111 and the second temperature regulating element 112, the less easily their temperatures are affected by the first heat insulation element 113 and the container 20, allowing the container 20 to accurately and quickly reach the first and second temperatures.

[0065] Furthermore, each of the first gaps D11 is configured to accommodate one or more of the containers 20 along a first preset direction. The first preset direction is the extending direction of the first gap D11, which is the relative direction between the first temperature regulating member 111 and the second temperature regulating member 112. That is, in one embodiment, one first gap D11 accommodates only one container 20, such as... Figure 3 and Figure 4 As shown. In another embodiment, a first gap D11 can accommodate multiple containers 20, the arrangement direction of the multiple containers 20 being the same as the extending direction of the first gap D11. "Multiple" means the number is greater than or equal to two, specifically two, four, five, six, etc. Figure 7 As shown. It should be noted that when in the first state, all containers 20 are located within the first gap D11, and when in the second state, all containers 20 are located within the second gap D12.

[0066] Please refer to Figure 8 and Figure 9 Two adjacent first heat transfer parts 1112 are used to contact the same container 20. That is, opposite sides of the container 20 are respectively in contact with two adjacent first heat transfer parts 1112, which can improve heat transfer efficiency. Optionally, both adjacent first heat transfer parts 1112 abut against the container 20 to hold the container 20, thereby improving the stability of the container 20 during the heating process.

[0067] For example, the container 20 has a first surface M1 and a second surface M2 that are opposite to each other. When the temperature circulation device 10 is in the first state, two adjacent first heat transfer parts 1112 can respectively contact the first surface M1 and the second surface M2.

[0068] Specifically, when container 20 is filled with reagent, it expands, causing the first surface M1 and the second surface M2 to abut against two adjacent first heat transfer parts 1112. Since both the first surface M1 and the second surface M2 are in contact with the first heat transfer parts 1112, there are two heat transfer paths between each container 20 and the first temperature regulating element 111. This allows the first temperature regulating element 111 to more easily transfer heat to the container 20, enabling the sample in the container 20 to be heated to the first temperature more quickly. In a further embodiment, while abutting against the two adjacent first heat transfer parts 1112, container 20 can also abut against the first connecting part 1111 forming the first gap D11, resulting in three heat transfer paths between each container 20 and the first temperature regulating element 111, further enabling the sample to be heated to the first temperature more quickly.

[0069] Similarly, two adjacent second heat transfer sections 1122 are used to contact the same container 20. Specifically, when the temperature circulation device 10 is in the second state, two adjacent second heat transfer sections 1122 can contact the first surface M1 and the second surface M2 respectively. Since both the first surface M1 and the second surface M2 are in contact with the second heat transfer section 1122, there are two heat transfer paths between each container 20 and the second temperature regulating element 112. The second temperature regulating element 112 and the container 20 can transfer heat more easily, and the sample in the container 20 can be cooled from the first temperature to the second temperature more quickly.

[0070] Optional, please refer to Figure 10 The temperature regulating component 11 may further include a third temperature regulating element 116. The third temperature regulating element 116 is used to adjust the sample temperature inside the container 20 to a third temperature. The third temperature regulating element 116 includes a plurality of third heat transfer sections 1162, which are spaced apart along a second preset direction to form a third gap D13. The third gap D13 constitutes a portion of the receiving channel D10. The temperature circulation device may also have a third state. When the temperature circulation device is in the third state, the container 20 is located within the third gap D13 and in contact with the third heat transfer section 1162 to adjust the sample temperature inside the container 20 to the third temperature. The third temperature is different from the first temperature and the second temperature.

[0071] Furthermore, the temperature regulating component 11 also includes a second heat insulation element 117, which includes a plurality of second sub-heat insulation parts 1171 connected to the third heat transfer part 1162 and the second heat transfer part 1122. The plurality of second sub-heat insulation parts 1171 are spaced apart along a second preset direction to form a second connecting channel L2. The second connecting channel L2 constitutes part of the receiving channel D10 and connects the third gap D13 and the second gap D12. The second heat insulation element 117 is used to reduce the heat transfer process between the third temperature regulating element 116 and the second temperature regulating element 112. It can be understood that after setting the third temperature regulating element 116, the sample temperature can cycle between the first temperature, the second temperature, and the third temperature, or between any two of the three temperatures, according to actual usage requirements, thus making it suitable for more application scenarios.

[0072] In some cases, please refer to Figure 11The temperature cycling device also includes an optical element 12, which is positioned on one side of the temperature control component 11 corresponding to the receiving channel D10. This optical element 12 is used to collect fluorescence generated during the biochemical amplification of the sample in the container 20. Therefore, the receiving channel D10 needs to penetrate the temperature control component 11 in a third preset direction (the third preset direction is perpendicular to the second and first preset directions) to facilitate fluorescence collection by the optical element 12. It is understandable that if the design of the receiving channel D10 or the container 20 is unreasonable, the container 20 may fall out of the receiving channel D10. The following is a brief introduction to solutions to overcome the problem of the container 20 falling out.

[0073] In one implementation, please refer to Figure 11 and Figure 12 The temperature control component 11 has a first inclined surface X1 and a second inclined surface X2 arranged opposite to each other. The first inclined surface X1 and the second inclined surface X2 form the sidewall of the receiving channel D10. The first inclined surface X1 and the second inclined surface X2 have different inclination directions. And in a third preset direction, the distance between the first inclined surface X1 and the second inclined surface X2 decreases from large to small. In other words, the receiving channel D10 formed by the first inclined surface X1 and the second inclined surface X2 is conical. When the container 20 is selected as a rigid container, the shape of the rigid container is conical to fit the conical receiving channel D10. When the container 20 is selected as a flexible container, the flexible container after being filled with reagent is conical to fit the conical receiving channel D10. The first inclined surface X1 and the second inclined surface X2 form a first opening K1 and a second opening K2. The first opening K1 is located at the point where the distance between the first inclined surface X1 and the second inclined surface X2 is the largest. The second opening K2 is located at the point where the distance between the first inclined surface X1 and the second inclined surface X2 is the smallest. The relative orientation of the first opening K1 and the second opening K2 is the third preset direction. When sample amplification is required, the container 20 can be inserted through the first opening K1. It is understood that, due to the smaller size of the second opening K2, the container 20 cannot fall through it, thus avoiding the problem of it falling. Furthermore, since both the first inclined surface X1 and the second inclined surface X2 are inclined surfaces, the contact area between the temperature control component 11 and the container 20 can be increased, thereby improving heat transfer efficiency. Of course, there are other implementation methods to prevent the container 20 from falling, which will not be detailed here.

[0074] Optionally, the temperature circulation device 10 may further include a power source for driving the temperature regulating component 11 to move, thereby changing the relative position of the temperature regulating component 11 and the container 20. The movement type of the temperature regulating component 11 is linear motion. The direction of movement of the temperature regulating component 11 is parallel to the relative direction of the first temperature regulating element 111 and the second temperature regulating element 112. The power output form of the power source can be linear motion, rotary motion, or other types. The power source can be, but is not limited to, an electromagnet, a linear motor, a cylinder, or a hydraulic cylinder. It is understood that when the power source output is rotary motion, it is necessary to convert the rotary motion into linear motion, for example, by using a lead screw to convert the rotary motion into linear motion.

[0075] Although embodiments of this application have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting this application. Those skilled in the art can make changes, modifications, substitutions and variations to the above embodiments within the scope of this application, and such improvements and refinements are also considered to be within the protection scope of this application.

Claims

1. A temperature circulation device, characterized in that, The temperature circulation device includes: a temperature regulating component for adjusting the temperature of a sample in a container; the temperature regulating component includes a first temperature regulating element and a second temperature regulating element, which together form a receiving channel for accommodating the container; the temperature regulating component and the container can move relative to each other in a first preset direction to change the position of the container within the receiving channel, thereby giving the temperature circulation device a first state and a second state; when the temperature circulation device is in the first state, the first temperature regulating element contacts the container to adjust the sample in the container to a first temperature; when the temperature circulation device is in the second state, the second temperature regulating element contacts the container to adjust the sample in the container to a second temperature; wherein the first temperature and the second temperature are different, and the first preset direction is the relative direction of the first temperature regulating element and the second temperature regulating element; The first temperature regulating element includes a plurality of first heat transfer parts, which are spaced apart along a second preset direction to form a first gap. The second preset direction is perpendicular to the first preset direction. The first gap forms part of the receiving channel. When the temperature circulation device is in the first state, the container is located in the first gap and in contact with the first heat transfer parts. The second temperature regulating element includes a plurality of second heat transfer parts, which are spaced apart along a second preset direction to form a second gap. The second gap forms part of the receiving channel. When the temperature circulation device is in the second state, the container is located in the second gap and in contact with the second heat transfer parts. The first gap and the second gap are arranged opposite each other and are interconnected. Each of the first gaps is used to accommodate one or more of the containers along a first preset direction. When the temperature circulation device is in the first state, the opposite sides of the containers are in contact with the first temperature regulating element; when the temperature circulation device is in the second state, the opposite sides of the containers are in contact with the second temperature regulating element.

2. The temperature circulation device as described in claim 1, characterized in that, The temperature regulating component further includes a first heat insulation element, which includes a plurality of first sub-heat insulation parts. The first sub-heat insulation parts are connected to the first heat transfer part and the second heat transfer part. The plurality of first sub-heat insulation parts are spaced apart along a second preset direction to form a first connecting channel. The first connecting channel forms part of the receiving channel and connects the first gap and the second gap. The first heat insulation element is used to reduce the heat transfer process between the first temperature regulating element and the second temperature regulating element.

3. The temperature circulation device as described in claim 1, characterized in that, The temperature control assembly further includes a first heating element for heating the first temperature control element, the first heating element being connected to each of the first heat transfer parts.

4. The temperature circulation device as described in claim 1, characterized in that, Two adjacent first heat transfer sections are used to contact the same container.

5. A detection device, characterized in that, The detection device includes a container and a temperature circulation device as described in any one of claims 1-4. The temperature regulating component of the temperature circulation device is movable relative to the container to give the temperature circulation device a first state and a second state. When the temperature circulation device is in the first state, the first temperature regulating element of the temperature regulating component contacts the container; when the temperature circulation device is in the second state, the second temperature regulating element of the temperature regulating component contacts the container.

6. The detection device as described in claim 5, characterized in that, When the temperature circulation device is in the first state, the opposite sides of the container are in contact with the first temperature regulating element; when the temperature circulation device is in the second state, the opposite sides of the container are in contact with the second temperature regulating element.

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