A recombinant collagen water-oil dual-phase essence oil and a preparation method thereof

Abstract

The application belongs to the technical field of cosmetics, and proposes a recombinant collagen water-oil dual-phase essence oil and a preparation method thereof, which comprises a water phase and an oil phase; the water phase comprises the following components: 0.01-1 parts of recombinant collagen, 0.01-1 parts of nonapeptide-1, 0.001-0.1 parts of superoxide dismutase, 0.01-2 parts of ginseng extract, 0.01-2 parts of snowbell flower extract, and water supplementing 100 parts; the oil phase comprises the following components: 10-35 parts of squalane, 20-50 parts of jojoba oil, 5-15 parts of cactus seed oil, 3-5 parts of white pool flower seed oil, 1-5 parts of baobab tree oil, 1-3 parts of sea buckthorn fruit oil, 1-6 parts of pomegranate seed oil, 1-3 parts of camellia oil, 0.5-3 parts of rosemary essential oil, and 1-5 parts of orange flower essential oil. Through formula optimization and supramolecular technology, the application solves the sticky short board of "oil" and can effectively promote the transdermal absorption of recombinant collagen.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of cosmetics, and relates to a recombinant collagen water-oil dual-phase essence oil and a preparation method thereof. BACKGROUND

[0002] The concept of "nourishing skin with oil" has attracted more and more people's interest in "oil". In principle, the concept of "nourishing skin with oil" is indeed reliable, which usually refers to using base oil, vegetable oil and natural essential oil to condition the skin to achieve the effect of moisturizing. However, the concept of "nourishing skin with oil" also has its shortcomings. For users with acne-prone skin and oily skin, problems such as too oily, acne, and closed pores often occur, and they express that they cannot bear "oil".

[0003] Modern skin care science believes that the skin has the ability to regulate water and oil balance. When the skin lacks water, the skin will not only be dry, but also cause oil secretion. Because the skin is short of water, the skin's self-protection mechanism is activated, which will lock in water by secreting oil to reduce the skin's continuous water loss. Therefore, the most fundamental logic of oil control is not just to remove oil, but to hydrate. Only when the water and oil are balanced, the skin will return to a healthy and good state. Therefore, the dual-phase essence capable of regulating the balance of water and oil can better care for the skin state. The "oil" in "nourishing skin with oil" mostly refers to plant extract oil. Extracted from plant seeds, pulp or germ, etc., the active ingredients inside are more abundant and more natural. Common skin care oils include tea tree oil, grape seed oil, rosehip oil, jojoba oil, etc. They contain oleic acid, linoleic acid, linolenic acid, etc. They are easier to absorb than mineral oil, and can moisturize and nourish the skin at the same time.

[0004] Collagen has the effects of pure natural moisturizing repair, whitening and freckle removal, wrinkle removal, etc., and can be widely used in beauty products. However, the transdermal absorption of external use collagen is difficult due to its large molecular weight, which has been a key issue questioned by people. Hydrolyzed collagen has too small molecular weight and unstable molecular structure, and is easily decomposed into amino acids, with low beauty conversion rate.

[0005] Therefore, in view of the deficiencies of existing essence oil products, it is a technical problem to be solved by those skilled in the art to develop a new type of water-oil dual-phase essence oil that is beneficial to the transdermal absorption of recombinant collagen macromolecules to meet the needs of dry, neutral and mixed skin. SUMMARY

[0006] The present application aims to overcome the defects in the prior art, and provides a water-oil dual-phase essence oil with reasonable formula, convenient to use, safe and comfortable, and effective in promoting the transdermal absorption of collagen, and a preparation method thereof.

[0007] The technical solution of the present application is as follows:

[0008] A kind of recombinant collagen water-oil dual-phase essence oil, including water phase and oil phase with weight ratio 1-7:2-6;

[0009] The water phase includes the following components by weight fraction:

[0010] Recombinant collagen 0.01-1 parts,

[0011] Nonapeptide-1 0.01-1 parts,

[0012] Superoxide dismutase 0.001-0.1 parts,

[0013] Centella asiatica extract 0.01-2 parts,

[0014] Edelweiss extract 0.01-2 parts,

[0015] Water is supplemented to 100 parts;

[0016] The oil phase includes the following components by weight fraction:

[0017] Squalane 10-35 parts,

[0018] Jojoba oil 20-50 parts,

[0019] Cactus seed oil 5-15 parts,

[0020] White pool flower seed oil 3-5 parts,

[0021] Baobab oil 1-5 parts,

[0022] Sea buckthorn fruit oil 1-3 parts,

[0023] Pomegranate seed oil 1-6 parts,

[0024] Camellia oil 1-3 parts,

[0025] Rosemary essential oil 0.5-3 parts,

[0026] Orange flower essential oil 1-5 parts.

[0027] As a preferred embodiment of the application, the recombinant collagen water-oil dual-phase essence oil includes water phase and oil phase with weight ratio 1:4;

[0028] The water phase includes the following components by weight fraction:

[0029] Recombinant collagen 0.01-0.3 parts,

[0030] Nonapeptide-1 0.1-1 parts,

[0031] Superoxide dismutase 0.005-0.05 parts,

[0032] Centella asiatica extract 0.05-0.1 parts,

[0033] Yarrowia extract 0.01-0.5 parts,

[0034] Water is added to 100 parts;

[0035] The oil phase includes the following components by mass fraction:

[0036] Squalane 30-35 parts,

[0037] Jojoba oil 40-45 parts,

[0038] Cactus seed oil 8-10 parts,

[0039] White pool flower seed oil 3-5 parts,

[0040] Monkey bread tree oil 2-5 parts,

[0041] Sea buckthorn fruit oil 2-3 parts,

[0042] Pomegranate seed oil 2-3 parts,

[0043] Camellia oil 1-2 parts,

[0044] Rosemary essential oil 1-3 parts,

[0045] Orange flower essential oil 3-5 parts.

[0046] As a preferred embodiment of the present application, the superoxide dismutase specific activity is 10000 U / mg-30000 U / mg.

[0047] As a preferred embodiment of the present application, the molecular weight of the recombinant collagen is 25 kDa-90 kDa.

[0048] The preparation method of the above-mentioned recombinant collagen water-oil dual-phase essence oil includes the following steps:

[0049] A: mix the components of the oil phase according to the formula amount, and obtain the oil phase;

[0050] B: mix the components of the water phase according to the formula amount, filter and sterilize, and obtain the water phase;

[0051] C: use a sterile filling process to fill the oil phase and the water phase into a secondary throwing pipe at a weight ratio of 1-7:2-6, seal, and obtain the product.

[0052] As a preferred embodiment of the present application, the mixing of step A is specifically carried out at a temperature of 20-30°C, a stirring rate of 100-300 rpm, and a stirring time of 4-5 min.

[0053] As a preferred embodiment of the present application, the mixing of step B is specifically carried out at a temperature of 20-30 DEG C, a stirring rate of 100-300 rpm, and a stirring time of 8-10 min.

[0054] The use method of the above-mentioned recombinant collagen water-oil dual-phase essence oil is to shake the essence oil to make it into a uniformly dispersed micro-water bead oil liquid essence, and then directly apply it to the face and massage it with hands until it is completely absorbed; wherein the shaking of the essence oil utilizes the supermolecular force to form a micro-water bead oil liquid essence, so that the water-soluble ingredients are dispersed and wrapped in the oily ingredients. After being applied to the face, the water-soluble ingredients enter the stratum corneum together with the oily ingredients, gradually release during penetration, promote the transdermal absorption of exogenous collagen molecules, and thus achieve the effects of better absorption and barrier repair of the product.

[0055] The working principle and beneficial effects of the present application are as follows:

[0056] 1. The present application provides a recombinant collagen water-oil dual-phase essence oil, which has the characteristics of good stability, moderate oil-water layering, and clear interface, with the upper layer being an oil phase and the lower layer being a water phase, and the recombinant collagen being stable in the water phase.

[0057] 2. The essence oil of the present application does not add emulsifiers, fragrances, and other ingredients, and does not cause additional burden to the skin. Research shows that emulsifiers usually have certain irritancy, and if the skin is relatively weak and long-term use of skin-expanding products containing emulsifiers, it may irritate the skin, damage the stratum corneum, make the skin sensitive, and trigger local red spots, erythema, cancer itching, swelling, and other allergic reactions. The water-oil separation dual-phase essence of the present application has many benefits. From the perspective of the user, because it reduces the addition of various solubilizers and emulsifiers, it is more friendly to sensitive skin. At the same time, it adds skin care ingredients, and through precise proportioning, it neutralizes the sticky skin feeling of oil, and even achieves the skin feeling of essence, which makes it friendly for users with oily skin, acne skin, dry skin, mixed skin, sensitive skin, etc.

[0058] 3. The essence oil of the present application optimizes the formula system and uses the supermolecular self-permeation technology, makes small molecules enter the skin with macromolecules through non-covalent bonds, and makes the water-soluble macromolecular active ingredients that are difficult to penetrate penetrate better, while also meeting the pursuit of skin feeling by consumers. Unlike the heavy feeling and difficulty of absorption caused by the pure oil formula of ordinary essence oil, it can well compatible with a variety of effective components that are soluble in water and oil.

[0059] Specifically, when in use, the liquid is shaken, and in the shaking process, the solution forms micro-water droplets of oil liquid essence, so that the water-soluble ingredients are uniformly dispersed and wrapped in the oily ingredients, and then enter the stratum corneum together with the oily ingredients, and the water-soluble ingredients are gradually released in the penetration, promoting the transdermal absorption of exogenous recombinant collagen molecules, so as to achieve better absorption and repair of the product.

[0060] Carrying recombinant collagen penetrates into the deep layer of the skin, and the oil blocking effect can further increase the action time of recombinant collagen in the water phase, increase the penetration of recombinant collagen, and the water-oil two-phase system can synergize the biological efficacy of recombinant collagen; the recombinant collagen can play a better effect in the water-oil two-phase system, such as repair, anti-wrinkle, tightening, soothing and the like.

[0061] 4、The essence oil of the present application is rich in various functional ingredients, which are mutually adjusted and synergistically act, so that it can prevent damage to the skin barrier, promote the repair process of the damaged skin barrier, and has good anti-allergic effect on sensitive skin with abnormal skin barrier function.

[0062] The oil phase adopts 10 kinds of scientific compound essential oil formulas to regulate the internal and external ecology of the skin from the exogenous and endogenous, on the one hand, to resist external invasion, and on the other hand, to strengthen the skin protection barrier. From the endogenous, the cell activity is increased, and from the exogenous, the skin barrier is constructed to resist the damage of external factors to the subcutaneous cells. Squalane + jojoba oil + cactus seed oil + sea buckthorn fruit oil can effectively maintain the stability of the skin, activate the metabolism of the skin, optimize and strengthen the barrier; white pool flower seed oil + baobab oil + pomegranate seed oil can gently regulate the internal and external environment of the skin, balance the problem of uneven water and oil in the skin, maintain and improve the water retention capacity of the skin, and make the skin smooth; orange flower essential oil + camellia oil + rosemary essential oil adopts plant extract essence to synergistically regulate the skin, help resist skin inflammation, and play a calming and soothing role.

[0063] The water phase adopts 5 kinds of water-soluble skin care ingredients to inhibit the signal transmission of tyrosinase from five dimensions, limit and reduce its activity and quantity, help repair and improve the skin dullness caused by light damage, assist the synthesis of collagen fibers and elastin, inhibit the signal transmission of collagen loss, effectively repair fine lines and wrinkles; improve the skin stratum corneum antioxidant system, reduce skin oxidative damage, enhance skin metabolism; block the transmission of sensitization signals, rebuild and consolidate the integrity of the skin barrier, reduce skin irritability; assist in blocking the glycosylation reaction, promote the reconstruction of collagen fiber network, and source repair glycosylation damage.

[0064] In the present application, the combination of jojoba oil + squalane + cactus seed oil + orange flower essential oil, on the one hand, promotes the transdermal absorption of water-soluble collagen macromolecules to play the anti-aging and wrinkle-removing effect, and on the other hand, synergistically with collagen to smooth fine lines and stabilize the skin barrier.

[0065] 5、The present application adopts sterile filling, does not contain any preservative, and is fresh, pure, sterile, sanitary and convenient. BRIEF DESCRIPTION OF DRAWINGS

[0066] Figure 1 Figure of water-oil dual-phase essence oil product of embodiment 5 of the present application;

[0067] In the figure: 1-before shaking; 2-after shaking. DETAILED DESCRIPTION

[0068] The technical solutions in the embodiments of the present application will be clearly and completely described below in combination with the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative labor fall within the scope of protection of the present application.

[0069] In the following examples and comparative examples, the recombinant collagen is prepared by the method disclosed in the invention patent with the name "Recombinant human type III collagen with functional structure and its expression method" of Chinese patent 201911384531.4. The superoxide dismutase is Cu, Zn-SOD obtained by the method disclosed in the patent publication 202211658617.3, with specific activity of 20000 U / mg.

[0070] The sources of other components in the examples are shown as follows, which can also be purchased from the market.

[0071]

[0072] Example 1

[0073] The composition of the recombinant collagen water-oil dual-phase essence oil of the present embodiment is as follows (weight fraction):

[0074]

[0075]

[0076] The preparation method is as follows:

[0077] A: Mix the components of the oil phase according to the formula amount, stir at 20℃ and 300rpm for 4min, mix well, and obtain the oil phase;

[0078] B: Mix and stir the components of the water phase according to the formula amount, stir at 20℃ and 300rpm for 10min, mix well, filter and sterilize, and obtain the water phase;

[0079] C: The oil phase and the water phase are filled into the secondary throwing pipe according to the weight ratio of 7:2 by using the sterile filling process, and the product is obtained after sealing.

[0080] Example 2

[0081] The composition of the recombinant collagen water-oil dual-phase essence oil of the present example is as follows (weight parts):

[0082]

[0083] The preparation method is as follows:

[0084] A: The components of the oil phase are mixed according to the formula amount, stirred at 30°C and 100 rpm for 5 min, and uniformly mixed, and the oil phase is obtained;

[0085] B: The components of the water phase are mixed and stirred according to the formula amount, stirred at 30°C and 100 rpm for 8 min, uniformly mixed, filtered and sterilized, and the water phase is obtained;

[0086] C: The oil phase and the water phase are filled into the secondary throwing pipe according to the weight ratio of 1:6 by using the sterile filling process, and the product is obtained after sealing. The skin feel is thick and heavy, and it is suitable for dry skin.

[0087] Example 3

[0088] The composition of the recombinant collagen water-oil dual-phase essence oil of the present example is as follows (weight parts):

[0089]

[0090] The preparation method is as follows:

[0091] A: The components of the oil phase are mixed according to the formula amount, stirred at 25°C and 200 rpm for 4 min, and uniformly mixed, and the oil phase is obtained;

[0092] B: The components of the water phase are mixed and stirred according to the formula amount, stirred at 25°C and 200 rpm for 9 min, uniformly mixed, filtered and sterilized, and the water phase is obtained;

[0093] C: The oil phase and the water phase are filled into the secondary throwing pipe according to the weight ratio of 1:4 by using the sterile filling process, and the product is obtained after sealing.

[0094] Example 4

[0095] The composition of the recombinant collagen water-oil dual-phase essence oil of the present example is as follows (weight parts):

[0096]

[0097] The preparation method is the same as that of Example 3, the weight ratio of the water phase and the oil phase is 7:3, the skin feel is refreshing, and it is suitable for oily skin.

[0098] Example 5

[0099] The composition of the recombinant collagen water-oil dual-phase essence oil of the present example is as follows (weight parts):

[0100]

[0101] The preparation method is the same as that of Example 3, and the weight ratio of the water phase to the oil phase is 1:2.

[0102] Comparative Example 1

[0103] The same as Example 3, except that only the water phase is present.

[0104] Comparative Example 2

[0105] The same as Example 3, except that only the oil phase is present, and equal amounts of recombinant collagen are added to the oil phase.

[0106] Comparative Example 3

[0107] The same as Example 3, except that the water-oil ratio is 1:7.

[0108] Comparative Example 4

[0109] The same as Example 3, except that the oil phase does not contain jojoba oil.

[0110] Comparative Example 5

[0111] The same as Example 3, except that the oil phase does not contain cactus seed oil or orange flower essential oil.

[0112] Comparative Example 6

[0113] The same as Example 3, except that the oil phase does not contain cactus seed oil.

[0114] Comparative Example 7

[0115] The same as Example 3, except that the oil phase does not contain orange flower essential oil.

[0116] Investigation of the speed of separation and skin feel of Example 1 after mixing

[0117] The mixing paddle of the blender is placed in the middle of the height of the essence liquid, and the product is stirred at a speed of 200 rpm for 1 min, and then left to separate. The time required for the product to stop stirring until there are no obvious oil droplets at the water-oil interface is measured. The essence oils obtained by mixing Examples 1-5 and Comparative Examples 1-7 are applied to the back of the hand, and the test results are shown in Table 1.

[0118] After the water-oil of Examples 1-5 is shaken, the separation speed is moderate, and the water-soluble ingredients wrapped by the supermolecular technology are released to play a role in the stratum corneum. The separation speed of the essence oil of the comparative examples is too fast or too slow, which is not conducive to the release of the efficacy of the active ingredients.

[0119] Table 1: Layering speed and skin feel test of each group of essential oil

[0120] Group Layering time Layering interface Skin feel Example 1 4min10s Layering interface clear Moisturizing degree moderate, refreshing, fast absorption Example 2 5min53s Layering interface clear Skin feel heavy, long duration Example 3 5min12s Layering interface clear Moisturizing degree moderate, moderate absorption Example 4 4min38s Layering interface clear Moisturizing degree moderate, refreshing, fast absorption Example 5 4min55s Layering interface clear Moisturizing degree moderate, relatively refreshing Comparative Example 1 - No layering Poor moisturizing degree Comparative Example 2 - Collagen not dissolved, settled at the bottom of the essential oil Sticky, heavy, collagen not absorbed Comparative Example 3 7min50s Layering interface clear Sticky, poor skin feel Comparative Example 4 11min18s Layering interface not clear Poor moisturizing degree, uncomfortable skin feel Comparative Example 5 8min41s Layering interface not clear Uncomfortable skin feel Comparative Example 6 8min15s Layering interface not clear Uncomfortable skin feel Comparative Example 7 5min41s Layering interface clear Skin feel acceptable

[0121] From the results of the above table, compared with Comparative Examples 1-7, the layering speed of the essential oil prepared in Examples 1-5 is moderate, the skin feel is good, and the efficacy of the active ingredients is beneficial to play. Comparative Example 3 and Comparative Examples 4 and 6 show that the synergistic effect of jojoba oil and cactus seed oil will affect the layering speed and layering interface clarity of the water-oil dual-phase essential oil.

[0122] Stability test of Investigation Example 2

[0123] Examples 1-5 and Comparative Examples 3-6, each test sample was set up 3 groups of experiments, respectively, under the conditions of cold and hot alternation (placed in a constant temperature box, the temperature in the constant temperature box was reciprocated from -20℃ to 30℃, specifically: the temperature in the constant temperature box was kept at -20℃ for 10h; then warmed from -20℃ to 30℃, the warming time was 1h; the temperature in the constant temperature box was kept at 30℃ for 10h; then cooled from 30℃ to -20℃, the cooling time was 1h; repeat the above cycle), room temperature condition (placed in a constant temperature box of 25℃), light condition (placed in a constant temperature box of 25℃ and simulated 24h light) for 30d, to observe whether each group of samples was layered, whether the lower liquid was turbid and other unstable phenomena.

[0124] The essential oil provided by Examples 1-5 can maintain the stable state of the bottom layer as a colorless liquid and the upper layer as a yellow liquid after 1 month of testing under cold and hot alternating, room temperature, and light conditions, and the layering is clear, without unstable phenomena such as turbidity of the bottom layer, indicating that the water-oil dual-phase essential oil provided by the present application has excellent stability. The essential oil provided by Comparative Examples 3, 4, 5, and 6 showed unstable phenomena such as turbidity of the lower liquid during testing under cold and hot alternating and room temperature conditions.

[0125] Safety evaluation of Investigation Example 3

[0126] The test and evaluation were carried out according to the skin irritation test in the 2015 edition of "Cosmetic Safety Technology Standard", specifically: about 24 h before the test, the experimental animals were shaved on both sides of the dorsal spine, without damaging the epidermis, and the shaved area was about 3 cm x 3 cm on both left and right sides. About 0.5 mL of the essential oil prepared in Example 3 was directly applied to the skin, then covered with two layers of gauze (2.5 cm x 2.5 cm), and then fixed with non-irritating adhesive tape and bandage. The other side of the skin served as a control. A closed test was used, and the application time was 2 h. After the test, the residual test substance was removed with warm water or a non-irritating solvent. The skin reaction at the application site was observed at 1, 24, 48, and 72 h after the test, and the skin reaction score was evaluated according to Table 2. The average value of the scores of the test animals was used for comprehensive evaluation, and the highest score at each observation time point was used to determine the skin irritation intensity according to Table 3.

[0127] Table 2 Skin irritation reaction score table

[0128]

[0129] Table 3 Skin irritation intensity classification

[0130]

[0131] Table 3 Product skin irritation reaction score results

[0132]

[0133] According to the product skin irritation reaction score results in Table 3, it was determined that the product had no skin irritation.

[0134] Transdermal absorption test of recombinant collagen in Investigation Example 4

[0135] According to the Franz diffusion cell test method in GB / T 27818-2011 "Chemical skin absorption in vitro test method", the isolated pig skin was washed with normal saline, clamped between the receiving chamber and the supply chamber of the Franz diffusion cell, with the outer epidermis of the skin facing the supply chamber. The water temperature system was adjusted to 32±1℃, the stirring rate was 200 r / min, PBS solution preheated to 32±1℃ was added to the receiving chamber, all bubbles were removed, the inner surface of the pig skin was in contact with the receiving liquid, and the test liquid was injected into the supply chamber and tightly attached to the surface of the pig skin. At 4 h and 12 h, the receiving liquid was removed with a syringe, filtered with a filter membrane with a pore size of 0.45 m, and the filtrate was used as the sample solution. The recombinant collagen control solution and the sample solutions of Examples 1-5 and Comparative Examples 1-7, as well as the blank matrix solution, were determined by HPLC, with a wavelength range of 220 nm. The transdermal absorption rate is shown in Table 4.

[0136] Table 4 Transdermal absorption test of each group of essential oil recombined collagen

[0137] Group Recombinant collagen 4h transdermal absorption rate (%) Recombinant collagen 12h transdermal absorption rate (%) Example 1 16.4％ 56.8％ Example 2 19.3％ 66.6％ Example 3 24.9％ 77.2％ Example 4 17.1％ 61.5％ Example 5 23.7％ 68.3％ Comparative Example 1 0 2.2％ Comparative Example 2 0 0 Comparative Example 3 12.1％ 41.6％ Comparative Example 4 10.2％ 30.7％ Comparative Example 5 12.6％ 32.9％ Comparative Example 6 13.7％ 35.5％ Comparative Example 7 16.1％ 55.8％

[0138] From the above data, the transdermal absorption of Examples 1-5 is much higher than that of Comparative Examples 1-7, indicating that the water-oil dual-phase essential oil formula of the present application is reasonable, and the components synergize to fully utilize the supramolecular self-permeation technology to solve the problem of transdermal absorption of recombined collagen macromolecules. The lack of components will affect the transdermal absorption of recombined collagen.

[0139] Human efficacy test of product of Example 5

[0140] The samples prepared from Examples 1-5 and Comparative Examples 1, 3, 4, 5, 6, and 7 were respectively sent to 110 test personnel for testing and use, aged between 25-55 years old, divided into 11 groups, 10 people in each group. The test personnel operated according to the use method of the claim, once a day. The cheek skin elasticity R2 value and tightness F4 value of the subjects on the 28th day were tested by the instrument Cutometer DUAL MPA580. The skin roughness parameter SEr value and skin wrinkle parameter SEw value of the subjects were tested by VisioScan VC20 plus, respectively. The values of the skin before and after using the sample were compared to evaluate the efficacy of the sample, and the test results are shown in Table 5. The results show that the components of the product of the present application synergistically play a role, and the removal of certain components will greatly reduce the efficacy of the product.

[0141] Table 5 Test results of skin efficacy

[0142]

[0143]

[0144] Note: Improvement rate (%) = (test value after using sample N days - test value before use) / test value before use * 100%

[0145] In summary, the recombined collagen water-oil dual-phase essential oil of the present application has good effects of moisturizing, soothing, tightening and smoothing the skin, anti-wrinkle and fine line lightening, and is suitable for various skin types, can effectively improve the skin elasticity and gloss, reduce roughness, and lighten wrinkles, making the skin smooth and delicate. The water-soluble components and oil-soluble components in the present application synergistically enhance each other, and play a good role of 1+1>2. The water-soluble components are released gradually with the penetration of the oil-soluble components into the stratum corneum, promoting the transdermal absorption of exogenous collagen molecules, thereby achieving better absorption and barrier repair of the product. Comparative Example 3 and Comparative Examples 5-7 show that the addition of cactus seed oil and orange flower essential oil makes the wrinkle-removing effect of the essential oil more significant, and the two synergistically enhance each other.

[0146] The above merely preferred embodiments of the present application are not used to limit the present application, any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application should be included in the protection scope of the present application.

Claims

1. A recombinant collagen water-oil biphasic serum oil, characterized in that, The water phase and the oil phase are in a weight ratio of 1-7:2-6; The water phase comprises the following components by weight fraction: Recombinant collagen 0.01-1 parts, Nonapeptide-1 0.01-1 parts, SOD 0.001-0.1 parts, Centella asiatica extract 0.01-2 parts, Edelweiss extract 0.01-2 parts, Water is supplemented to 100 parts; The oil phase comprises the following components by weight fraction: Squalane 10-35 parts, Jojoba oil 20-50 parts, Cactus seed oil 5-15 parts, White pool flower seed oil 3-5 parts, Baobab oil 1-5 parts, Sea buckthorn fruit oil 1-3 parts, Pomegranate seed oil 1-6 parts, Camellia oil 1-3 parts, Rosemary essential oil 0.5-3 parts, Orange flower essential oil 1-5 parts; The amino acid sequence of the recombinant collagen is shown in SEQ ID NO: 1 of CN 201911384531.4; the amino acid sequence of the SOD is shown in SEQ ID NO: 3 of CN 202211658617.

3.

2. The recombinant collagen water-oil biphasic serum oil according to claim 1, characterized in that, The water phase and the oil phase are in a weight ratio of 1:4; The water phase comprises the following components by weight fraction: Recombinant collagen 0.01-0.3 parts, Nonapeptide-1 0.1-1 parts, SOD 0.005-0.05 parts, Centella asiatica extract 0.05-0.1 parts, Edelweiss extract 0.01-0.5 parts, Water is supplemented to 100 parts; The oil phase comprises the following components by weight fraction: Squalane 30-35 parts, Jojoba oil 40-45 parts, Cactus seed oil 8-10 parts, White pool flower seed oil 3-5 parts, Baobab oil 2-5 parts, Sea buckthorn fruit oil 2-3 parts, Pomegranate seed oil 2-3 parts, Camellia oil 1-2 parts, Rosemary essential oil 1-3 parts, Orange flower essential oil 3-5 parts.

3. The recombinant collagen water-oil biphasic serum oil according to claim 1, characterized in that, The specific activity of the SOD is 10000 U / mg-30000 U / mg.

4. The recombinant collagen water-oil biphasic serum oil according to claim 1, characterized in that, The molecular weight of the recombinant collagen is 25 kDa-90 kDa.

5. The method for preparing a recombinant collagen water-oil dual-phase essence oil as described in claim 1, characterized in that, The method comprises the following steps: A: mixing the components of the oil phase according to the formula amount to obtain the oil phase; B: mixing the components of the water phase according to the formula amount, filtering and sterilizing to obtain the water phase; C: using a sterile filling process to fill the oil phase and the water phase into a secondary throwing pipe in a weight ratio of 1-7:2-6, and sealing to obtain the product.

6. The method for preparing a recombinant collagen water-oil dual-phase essence oil according to claim 5, characterized in that, The mixing of step A is specifically carried out at a temperature of 20-30°C, a stirring speed of 100-300 rpm, and a stirring time of 4-5 min.

7. The method for preparing a recombinant collagen water-oil dual-phase essence oil according to claim 5, characterized in that, The mixing of step B is specifically carried out at a temperature of 20-30°C, a stirring speed of 100-300 rpm, and a stirring time of 8-10 min.

8. The method of using a recombinant collagen water-oil biphasic serum oil of claim 1, wherein, The use method is to shake the essence oil to make it into a uniformly dispersed micro-water bead oil essence.

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