A wrinkle-resistant, firming and repairing cosmetic composition and its preparation method and application

Abstract

The present invention provides an anti-wrinkle, firming and repairing cosmetic composition and its preparation method and application, and relates to the technical field of cosmetics. The ingredients of the cosmetic composition include a hexapeptide composition, a firming tripeptide composition, an anti-wrinkle oligopeptide composition, a collagen peptide composition and an olive essence composition. Each component is compounded under a specific weight portion, and has the effects of synergistic full-effect anti-wrinkle, firming, delicate skin, anti-photoaging and repair. The cosmetic composition can be applied to the preparation of cosmetics. The prepared cosmetics are safe and non-irritating, soothing and non-greasy on the face, and have a good skin feel; and can promote skin cell metabolism, prevent the formation of fine lines and wrinkles, inhibit and dilute fine lines, wrinkles, effectively alleviate and comprehensively improve skin roughness, reduce skin wrinkles, and make the skin more firm; it has a significant effect of repairing the skin barrier.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics, and in particular relates to an anti-wrinkle, firming and repairing cosmetic composition and a preparation method and application thereof. Background Art

[0002] As people age, the collagen in their skin gradually disappears, and the reduction of subcutaneous fat causes the skin's elasticity to deteriorate, and even wrinkles and sagging may occur. Furthermore, staying up late, not eating on time, or not eating enough nutrition can all cause certain aging-like skin changes. In addition, sun exposure can damage the skin's tissues, making it more prone to dryness and flaking, causing skin discomfort. Researchers have investigated the causes of sagging, wrinkles, and damage to the skin, and the main reasons are: (1) collagen and elastin damage and loss caused by aging; (2) slow production of collagen and elastin; (3) photoaging caused by ultraviolet rays, increased metalloproteinases that damage collagen, or ultraviolet rays that cause barrier damage and inflammation; (4) expression lines caused by facial expressions. In order to slow down the formation or aggravation of wrinkles and make the skin firm and delicate, consumers have increasingly higher requirements for cosmetics.

[0003] With the improvement of people's awareness of skin care and the pursuit of beautiful skin quality, when choosing skin care products, on the one hand, they pursue green, natural, mild, safe and environmentally friendly skin care products, and on the other hand, they require the selected skin care products to have multiple functions and take effect quickly while achieving better results. The effects of cosmetics on the market are mostly predictable effects brought by the mixture of different raw materials. It is often easy to have the problem that after combining multiple cosmetic raw materials together, not only do they fail to exert multiple effects, but their effects become worse. For example, in the research and sales of anti-wrinkle, firming and repairing cosmetics, it is found that the components of cosmetics are mostly single peptides or a combination of multiple peptides.

[0004] Chinese invention patent CN115154354A discloses a firming and anti-wrinkle composition, a preparation method and application thereof, wherein the firming and anti-wrinkle composition comprises the following components in parts by mass: 5-40 parts of hydrolyzed plant protein, 0.02-0.1 parts of acetyl hexapeptide-8, and 0.001-0.12 parts of palmitoyl pentapeptide-4; wherein the compounding of acetyl hexapeptide-8 and palmitoyl pentapeptide-4 can inhibit aging and stimulate collagen resynthesis, and then compounding with hydrolyzed plant protein can reduce collagen degradation, and finally achieve collagen increment, thereby achieving a more efficient skin firming and anti-aging effect; at the same time, parahydroxyacetophenone and 1,2-hexanediol are added to the composition, both of which can replace traditional preservatives, thereby significantly improving the safety of the composition.

[0005] Chinese invention patent CN115645343B provides an antioxidant repairing composition containing pear flower extract, tea polyphenols and peptides and a preparation method thereof, wherein the composition comprises the following components in weight percentage: pear flower extract solution 1-15%, tea polyphenols 1-5%, tetrahydromethylpyrimidine carboxylic acid 0.1-7%, aloe vera leaf juice solution 1-15%, oligopeptide-3 solution 0.1-5%, oligopeptide-1 solution 0.1-5%, tripeptide-1 copper solution 0.1-5%, butanediol 1-30%, phenoxyethanol solution 0.5-5%, deionized water 13-94%. The composition is based on pear flower extract, tea polyphenols and aloe vera leaf juice, and is compounded with oligopeptide-3, oligopeptide-1 and tripeptide-1 copper, which has a synergistic antioxidant repairing effect.

[0006] Currently, products on the market generally choose 1-2 of the above reasons as entry points. Due to the limitations of cost and raw material component concentration, the effects are average and slow to take effect. The main reasons are: on the one hand, the peptide content is difficult to control. High-content peptides are often difficult to absorb for ordinary skin, and high-concentration peptides may also irritate the skin during the repair process, but low-concentration peptides are difficult to achieve the expected effect; on the other hand, the combination of multiple peptides and moisturizing ingredients or active extracts is likely to cause sensitive skin problems during the skin repair process. Summary of the invention

[0007] In view of the problems existing in the prior art, the present invention provides an anti-wrinkle, firming and repairing cosmetic composition and a preparation method and application thereof. The ingredients of the cosmetic composition include a hexapeptide composition, a firming tripeptide composition, an anti-wrinkle oligopeptide composition, a collagen peptide composition and an olive essence composition. The components are compounded at specific weight portions and have synergistic and all-round effects of anti-wrinkle, firming, refining skin, scavenging free radicals, resisting photoaging and repairing skin barrier function, with quick effect and excellent effect. The cosmetics are non-irritating, soothing and non-greasy on the face, and have a good skin feel.

[0008] To achieve the above purpose, the technical solution adopted by the present invention is as follows:

[0009] First, the present invention provides an anti-wrinkle, firming and repairing cosmetic composition, which includes the following components by weight: 0.5-15 parts of a hexapeptide composition, 0.5-5 parts of a firming tripeptide composition, 0.5-5 parts of an anti-wrinkle oligopeptide composition, 0.5-5 parts of a collagen peptide composition and 0.1-2 parts of an olive extract composition.

[0010] Further preferably, the cosmetic composition comprises the following components in parts by weight: 0.8-10 parts of a hexapeptide composition, 0.8-3 parts of a skin-firming tripeptide composition, 0.8-3 parts of an anti-wrinkle oligopeptide composition, 0.8-3 parts of a collagen peptide composition and 0.5-1.5 parts of an olive extract composition.

[0011] More preferably, the cosmetic composition comprises the following components in parts by weight: 1-5 parts of a hexapeptide composition, 1-3 parts of a skin-firming tripeptide composition, 1-3 parts of an anti-wrinkle oligopeptide composition, 0.8-2 parts of a collagen peptide composition and 0.8-1.2 parts of an olive extract composition.

[0012] Preferably, the hexapeptide composition comprises the following components: acetyl hexapeptide-8, caprylyl glycol and water.

[0013] Further preferably, the hexapeptide composition comprises the following components by weight percentage: 0.03-1% acetyl hexapeptide-8, 0.1-1% caprylyl glycol and the balance water.

[0014] More preferably, the hexapeptide composition comprises the following components by weight percentage: 0.045-0.055% acetyl hexapeptide-8, 0.45-0.55% caprylyl glycol and the balance water.

[0015] Most preferably, the hexapeptide composition comprises the following components by weight percentage: 0.05% acetyl hexapeptide-8, 0.5% caprylyl glycol and the balance water.

[0016] Preferably, the skin-tightening tripeptide composition comprises the following components: trifluoroacetyl tripeptide-2, dextran, glycerin and water.

[0017] Further preferably, the skin-tightening tripeptide composition comprises the following components by weight percentage: 0.01-0.05% trifluoroacetyl tripeptide-2, 0.05-0.1% dextran, 60-80% glycerol and the balance water.

[0018] More preferably, the skin-tightening tripeptide composition comprises the following components by weight percentage: 0.025% trifluoroacetyl tripeptide-2, 0.08% dextran, 65% glycerol and the balance water.

[0019] Preferably, the anti-wrinkle oligopeptide composition comprises the following components: oligopeptide-1, caproic acid, dextran, glycerol and water.

[0020] Further preferably, the anti-wrinkle oligopeptide composition comprises the following components by weight percentage: 1-10% oligopeptide-1, 1-10% caproic acid, 1-10% dextran, 20-80% glycerol and the balance water.

[0021] More preferably, the anti-wrinkle oligopeptide composition comprises the following components by weight percentage: 5% oligopeptide-1, 5% caproic acid, 5% dextran, 60% glycerol and the balance water.

[0022] Preferably, the collagen peptide composition comprises the following components: palmitoyl tripeptide-5, glycerin and water.

[0023] Further preferably, the collagen peptide composition comprises the following components by weight percentage: 0.5-2% palmitoyl tripeptide-5, 50-60% glycerol and the balance water.

[0024] More preferably, the collagen peptide composition comprises the following components by weight percentage: 1% palmitoyl tripeptide-5, 51% glycerol and the balance water.

[0025] Preferably, the olive extract composition comprises the following components: olive leaf extract, fructose and water.

[0026] Further preferably, the olive extract composition comprises the following components by weight percentage: 5-10% olive leaf extract, 50-70% fructose and the remainder water.

[0027] More preferably, the olive extract composition comprises the following components by weight percentage: 7.5% olive leaf extract, 60% fructose and the remainder water.

[0028] Then, the present invention provides a method for preparing the above cosmetic composition, comprising the steps of:

[0029] S1. Mix the raw materials of each component according to weight percentage, stir, and respectively prepare a hexapeptide composition, a skin-tightening tripeptide composition, an anti-wrinkle oligopeptide composition, a collagen peptide composition, and an olive extract composition;

[0030] S2, mixing the hexapeptide composition with the olive extract composition, stirring to obtain a mixture 1;

[0031] S3. Mix the mixture 1 with the skin-tightening tripeptide composition, the anti-wrinkle oligopeptide composition, and the collagen peptide composition to obtain a cosmetic composition.

[0032] Preferably, in step S2, the stirring temperature is 20-30°C, and the stirring time is 5-20 min.

[0033] More preferably, the stirring temperature is 25-28° C., and the stirring time is 8-15 min.

[0034] Preferably, in step S3, the stirring temperature is 20-30° C., and the stirring time is 20-60 min.

[0035] More preferably, the stirring temperature is 25-28° C., and the stirring time is 30-45 min.

[0036] Furthermore, the present invention provides the use of the above-mentioned cosmetic composition in preparing cosmetics with comprehensive anti-wrinkle, firming, delicate skin and repairing effects.

[0037] Furthermore, the present invention provides the use of the above-mentioned cosmetic composition in preparing cosmetics having the efficacy of preventing or improving sagging, roughness and aging.

[0038] In the present invention, the sagging, roughness and aging refer to one or more of forehead wrinkles, nasolabial folds, crow's feet, wrinkles around the lips, rough skin, dry skin and skin desquamation.

[0039] Finally, the present invention provides a cosmetic comprising the above cosmetic composition and conventional cosmetic basic ingredients.

[0040] Preferably, the cosmetics may be toners, lotions, essences, creams, ointments and facial masks.

[0041] More preferably, the cosmetics may be toner or facial cream.

[0042] Preferably, the conventional cosmetic basic ingredients include, but are not limited to, commonly used moisturizing ingredients, emulsifiers, thickeners, and antibacterial agents.

[0043] Compared with the prior art, the present invention has the following beneficial effects:

[0044] 1. The cosmetic composition of the present invention combines a hexapeptide composition, a firming tripeptide composition, an anti-wrinkle oligopeptide composition, a collagen peptide composition, and an olive essence composition in specific dosage ratios, so that the cosmetic composition has synergistic and comprehensive anti-wrinkle, firming and repairing skin, anti-oxidation, and improvement of skin sagging and aging effects.

[0045] 2. The cosmetic composition of the present invention is applied to the preparation of cosmetics. The prepared cosmetics can promote skin cell metabolism, inhibit and reduce wrinkles and fine lines, prevent the generation of fine lines and wrinkles, effectively alleviate and comprehensively improve various skin problems such as rough skin, dry lines and fine lines, sagging, etc., reduce skin wrinkles and make the skin more firm, and have a good skin feel, refreshing, non-greasy, and non-tight.

[0046] 3. The raw material formula of each component in the cosmetic composition of the present invention is reasonable. Acetyl hexapeptide-8, palmitoyl tripeptide-5, oligopeptide-1, trifluoroacetyl tripeptide-2, olive leaf extract and other basic solvent components are matched with each other, and the raw material price is low. Moreover, the obtained hexapeptide composition, skin-tightening tripeptide composition, anti-wrinkle oligopeptide composition, collagen peptide composition and olive essence composition are combined, and the prepared cosmetic composition has fast effects in anti-wrinkle, firming and repairing skin, anti-oxidation, and improving skin sagging and aging; the full-effect anti-wrinkle and firming repair effect is strong; after using the cosmetics, the skin is stable and no sensitivity problems occur. DETAILED DESCRIPTION

[0047] The following non-limiting examples can enable those skilled in the art to more fully understand the present invention, but do not limit the present invention in any way. The following content is merely an exemplary description of the scope of the present invention, and those skilled in the art can make various changes and modifications to the present invention based on the disclosed content, which should also fall within the scope of the present invention.

[0048] When the embodiments give numerical ranges, it should be understood that, unless otherwise specified in the present invention, both endpoints of each numerical range and any numerical value between the two endpoints can be selected. Unless otherwise defined, all technical and scientific terms used in the present invention have the same meanings as those generally understood by those of ordinary skill in the art to which the present invention belongs.

[0049] The present invention is further described below in the form of specific embodiments. The various chemical reagents used in the embodiments of the present invention are obtained through conventional commercial channels unless otherwise specified. In the following embodiments, the acetyl hexapeptide-8 was purchased from Shanghai Baihaobo Biotechnology Co., Ltd. with the item number CM9010; the palmitoyl tripeptide-5 was purchased from Qingdao Jesmin Trading Co., Ltd. with the item number UQ20352009; the oligopeptide-1 was purchased from Beijing Hanlinchao Chemical Co., Ltd. with the item number 769017443K; the trifluoroacetyl tripeptide-2 was purchased from Beijing Hanlinchao Chemical Co., Ltd. with the item number 820057669K; the olive leaf extract was purchased from Shanghai Ronghai Biotechnology Co., Ltd. with the item number H055; the tea polyphenols were purchased from Shaanxi Senyue Biotechnology Co., Ltd. with a purity of ≥98%, and the raw material source was green tea. Products from different manufacturers do not have a significant effect on the effect.

[0050] In the present invention, hexapeptide, also known as acetyl hexapeptide-8, is a neurotransmitter inhibitory peptide (active polypeptide) and a low-molecular peptide called "botulinum toxin peptide". This substance reduces muscle contraction by inhibiting the release of the neurotransmitter acetylcholine, thereby reducing the generation of dynamic wrinkles and expression lines. It can reduce existing facial wrinkles and effectively prevent the generation of new wrinkles. It has high anti-wrinkle activity and few side effects, and is used in high-end cosmetics series.

[0051] In the present invention, the collagen peptide is also called palmitoyl tripeptide-5, which prevents collagen degradation and promotes collagen regeneration to achieve anti-wrinkle effects.

[0052] In the present invention, the anti-wrinkle oligopeptide, also known as oligopeptide-1, is a four-amino acid biomimetic peptide (i.e., a molecule in nature that is synthetically replicated in a laboratory) that replicates a growth factor that promotes the production of key components at the skin epidermal junction. These key components are important skin structure-generating proteins, such as collagen VII, laminin-5, and fibronectin. Oligopeptide-1 achieves excellent anti-wrinkle effects mainly by promoting the production of two proteins: collagen VII and laminin-5.

[0053] In the present invention, the skin-tightening tripeptide is also called trifluoroacetyl tripeptide-2. Trifluoroacetyl tripeptide-2 is a polypeptide synthesized from three amino acids, which works by inhibiting the production of progerin, a cell aging promoter protein. Studies have found that trifluoroacetyl tripeptide-2 can restore cell activity to the level of young cells of 20 years old. This ingredient can well increase skin elasticity, firmness and reduce skin sagging.

[0054] In the present invention, the olive leaf extract is an extract obtained by ultrasonic extraction (cell wall breaking technology) from specific Italian olive leaves, which is titrated into oleuropein. The olive leaf extract is a skin antioxidant, repairing and soothing agent.

[0055] The cosmetic composition of the present invention can be applied by those skilled in the art to the preparation of cosmetics according to actual needs, and the cosmetics are not limited to toners, lotions, essences, creams, ointments and masks.

[0056] Example 1

[0057] A cosmetic composition, comprising the following components in parts by weight:

[0058]

[0059] The preparation method of the above-mentioned cosmetic composition comprises the steps of:

[0060] S1. According to the above weight percentages, the raw materials of each component are mixed and stirred to prepare a hexapeptide composition, a skin-tightening tripeptide composition, an anti-wrinkle oligopeptide composition, a collagen peptide composition, and an olive extract composition;

[0061] S2, mixing the hexapeptide composition with the olive extract composition, stirring at room temperature for about 15 minutes until the mixture is uniform, to obtain a mixture 1;

[0062] S3. The mixture 1 is sequentially mixed with the skin-tightening tripeptide composition, the anti-wrinkle oligopeptide composition, and the collagen peptide composition obtained in step S1, and stirred at room temperature for about 40 minutes to obtain a cosmetic composition 1.

[0063] The obtained cosmetic composition is used to prepare essence water. The preparation method of essence water includes the steps of: taking the above-mentioned cosmetic composition 1 and mixing it with conventional cosmetic basic ingredients, stirring at room temperature for about 40 minutes until it is uniform, and obtaining essence water; in the essence water, the cosmetic composition accounts for 5% and the conventional cosmetic basic ingredients account for 95%.

[0064] The above-mentioned conventional cosmetic basic ingredients, each ingredient and the weight percentage of each ingredient are specifically: sodium hyaluronate 0.5%, tataric acid bean polysaccharide 0.5%, propylene glycol 3%, hexylene glycol 0.6%, cynarin 0.6%, and water 89.8%.

[0065] Example 2

[0066] A cosmetic composition, comprising the following components in parts by weight:

[0067]

[0068] The preparation method of the above-mentioned cosmetic composition comprises the steps of:

[0069] S1. According to the above weight percentages, the raw materials of each component are mixed and stirred to prepare a hexapeptide composition, a skin-tightening tripeptide composition, an anti-wrinkle oligopeptide composition, a collagen peptide composition, and an olive extract composition;

[0070] S2, mixing the hexapeptide composition with the olive extract composition, stirring at room temperature for about 20 minutes until the mixture is uniform, to obtain a mixture 1;

[0071] S3. Mix the mixture 1 with the skin-tightening tripeptide composition, the anti-wrinkle oligopeptide composition, and the collagen peptide composition obtained in step S1 in sequence, and stir for about 45 minutes to obtain a cosmetic composition 2.

[0072] The obtained cosmetic composition is used to prepare a facial cream. The preparation method of the facial cream comprises the steps of: taking the above-mentioned cosmetic composition 2 and mixing it with conventional cosmetic basic ingredients, stirring at room temperature for about 40 minutes until it is uniform, and obtaining a facial cream; in the facial cream, the cosmetic composition accounts for 9% and the conventional cosmetic basic ingredients account for 91%.

[0073] The above-mentioned conventional cosmetic basic ingredients, each ingredient and the weight percentage of each ingredient are specifically: jojoba oil 5%, squalane oil 5%, shea butter 3%, emulsifier 3%, lecithin emulsifier 1%, propylene glycol 3%, hexylene glycol 0.6%, fennel 0.6%, water 69.8%.

[0074] Example 3

[0075] A cosmetic composition, comprising the following components in parts by weight:

[0076]

[0077] The preparation method of the above cosmetic composition is the same as that of Example 1 to obtain cosmetic composition 3.

[0078] The obtained cosmetic composition is used to prepare essence water. The preparation method of essence water includes the steps of: taking the above-mentioned cosmetic composition 3 and mixing it with conventional cosmetic basic ingredients, stirring at room temperature for about 40 minutes until it is uniform, and obtaining essence water; in the essence water, the cosmetic composition accounts for 5% and the conventional cosmetic basic ingredients account for 95%.

[0079] The above conventional cosmetic basic ingredients, each ingredient and the weight percentage of each ingredient are the same as those in Example 1.

[0080] Example 4

[0081] A cosmetic composition, comprising the following components in parts by weight:

[0082]

[0083] The preparation method of the above cosmetic composition is the same as that of Example 1 to obtain cosmetic composition 4.

[0084] The obtained cosmetic composition is used to prepare essence water. The preparation method of essence water includes the steps of: taking the above cosmetic composition 4 and mixing it with conventional cosmetic basic ingredients, stirring at room temperature for about 40 minutes until it is uniform, to obtain essence water; in the essence water, the cosmetic composition accounts for 5% and the conventional cosmetic basic ingredients account for 95%.

[0085] The above conventional cosmetic basic ingredients, each ingredient and the weight percentage of each ingredient are the same as those in Example 1.

[0086] Example 5

[0087] A cosmetic composition, comprising the following components in parts by weight:

[0088]

[0089] The preparation method of the above cosmetic composition is the same as that of Example 1 to obtain cosmetic composition 5.

[0090] The obtained cosmetic composition is used to prepare essence water. The preparation method of essence water includes the steps of: taking the above cosmetic composition 5 and mixing it with conventional cosmetic basic ingredients, stirring at room temperature for about 40 minutes until it is uniform, to obtain essence water; in the essence water, the cosmetic composition accounts for 10% and the conventional cosmetic basic ingredients account for 90%.

[0091] The above-mentioned conventional cosmetic basic ingredients, each ingredient and the weight percentage of each ingredient are specifically: sodium hyaluronate 0.25%, tataric acid bean polysaccharide 0.25%, propylene glycol 1.5%, hexylene glycol 0.3%, cinnamaldehyde 0.3%, and water 87.4%.

[0092] Example 6

[0093] A cosmetic composition, comprising the following components in parts by weight:

[0094]

[0095] The preparation method of the above cosmetic composition is the same as that of Example 1 to obtain cosmetic composition 6.

[0096] The obtained cosmetic composition is used to prepare essence water. The preparation method of essence water includes the steps of: taking the above-mentioned cosmetic composition 6 and mixing it with conventional cosmetic basic ingredients, stirring at room temperature for about 40 minutes until it is uniform, to obtain essence water; in the essence water, the cosmetic composition accounts for 5% and the conventional cosmetic basic ingredients account for 95%.

[0097] The above conventional cosmetic basic ingredients, each ingredient and the weight percentage of each ingredient are the same as those in Example 1.

[0098] Example 7

[0099] A cosmetic composition, comprising the following components in parts by weight:

[0100]

[0101] The preparation method of the above cosmetic composition is the same as that of Example 1 to obtain cosmetic composition 7.

[0102] The obtained cosmetic composition is used to prepare essence water. The preparation method of essence water includes the steps of: taking the above-mentioned cosmetic composition 7 and mixing it with conventional cosmetic basic ingredients, stirring at room temperature for about 40 minutes until it is uniform, and obtaining essence water; in the essence water, the cosmetic composition accounts for 5% and the conventional cosmetic basic ingredients account for 95%.

[0103] The above conventional cosmetic basic ingredients, each ingredient and the weight percentage of each ingredient are the same as those in Example 1.

[0104] Comparative Example 1

[0105] Different from Example 1, the components of the cosmetic composition are only the hexapeptide composition and the anti-wrinkle oligopeptide composition:

[0106]

[0107] The remaining parameters were the same as those in Example 1 to obtain essence water.

[0108] A method for preparing a cosmetic composition comprises the steps of:

[0109] S1. Mix the raw materials of each component according to the above weight percentages, stir, and prepare a hexapeptide composition and an anti-wrinkle oligopeptide composition respectively;

[0110] S2. Mix the hexapeptide composition and the anti-wrinkle oligopeptide composition, and stir at room temperature for about 40 minutes to obtain a cosmetic composition 8.

[0111] The obtained cosmetic composition is used to prepare essence water, and the preparation method of essence water includes the steps of: taking the above cosmetic composition 8 and mixing with conventional cosmetic basic ingredients, stirring at room temperature for about 40 minutes until uniform, to obtain essence water; in the essence water, the cosmetic composition accounts for 5%, and the conventional cosmetic basic ingredients account for 95%. The conventional cosmetic basic components are the same as those in Example 1.

[0112] Comparative Example 2

[0113] Different from Example 1, the components of the cosmetic composition are only the hexapeptide composition, the skin-tightening tripeptide composition and the anti-wrinkle oligopeptide composition:

[0114]

[0115] The remaining parameters were the same as those in Example 1 to obtain essence water.

[0116] A method for preparing a cosmetic composition comprises the steps of:

[0117] S1. According to the above weight percentages, the raw materials of each component are mixed and stirred to prepare a hexapeptide composition, a skin-tightening tripeptide composition, and an anti-wrinkle oligopeptide composition respectively;

[0118] S2. The hexapeptide composition, the skin-tightening tripeptide composition, and the anti-wrinkle oligopeptide composition are sequentially mixed, and stirred at room temperature for about 40 minutes to obtain a cosmetic composition 9.

[0119] The obtained cosmetic composition is used to prepare essence water, and the preparation method of essence water includes the steps of: taking the above cosmetic composition 9 and mixing it with conventional cosmetic basic ingredients, stirring at room temperature for about 40 minutes until it is uniform, and obtaining essence water; in the essence water, the cosmetic composition accounts for 5%, and the conventional cosmetic basic ingredients account for 95%. The conventional cosmetic basic components are the same as those in Example 1.

[0120] Comparative Example 3

[0121] Different from Example 1, the components of the cosmetic composition are only the hexapeptide composition, the anti-wrinkle oligopeptide composition and the olive extract composition:

[0122]

[0123] The remaining parameters were the same as those in Example 1 to obtain essence water.

[0124] A method for preparing a cosmetic composition comprises the steps of:

[0125] S1. According to the above weight percentages, the raw materials of each component are mixed and stirred to prepare a hexapeptide composition, an anti-wrinkle oligopeptide composition, and an olive extract composition respectively;

[0126] S2, mixing the hexapeptide composition with the olive extract composition, stirring at room temperature for about 15 minutes until the mixture is uniform, to obtain a mixture 1;

[0127] S3. The mixture 1 is sequentially mixed with the skin-tightening tripeptide composition and the anti-wrinkle oligopeptide composition obtained in step S1, and stirred at room temperature for about 40 minutes to obtain a cosmetic composition 10.

[0128] The obtained cosmetic composition is used to prepare essence water, and the preparation method of essence water includes the steps of: taking the above cosmetic composition 10 and mixing it with conventional cosmetic basic ingredients, stirring at room temperature for about 40 minutes until it is uniform, and obtaining essence water; in the essence water, the cosmetic composition accounts for 5%, and the conventional cosmetic basic ingredients account for 95%. The conventional cosmetic basic components are the same as those in Example 1.

[0129] Comparative Example 4

[0130] Different from Example 2, the components of the cosmetic composition are only the hexapeptide composition, the skin-tightening tripeptide composition and the collagen peptide composition:

[0131]

[0132] The remaining parameters were the same as those in Example 2 to prepare a facial cream.

[0133] A method for preparing a cosmetic composition comprises the steps of:

[0134] S1. According to the above weight percentages, the raw materials of each component are mixed and stirred to prepare a hexapeptide composition, a skin-tightening tripeptide composition, and a collagen peptide composition respectively;

[0135] S2. The hexapeptide composition, the skin-tightening tripeptide composition, and the collagen peptide composition are sequentially mixed and stirred for about 45 minutes to obtain a cosmetic composition 11.

[0136] The obtained cosmetic composition is used to prepare a face cream, and the preparation method of the face cream comprises the steps of: taking the above cosmetic composition 11 and mixing it with a conventional cosmetic basic component, stirring at room temperature for about 40 minutes until it is uniform, and obtaining a face cream; in the face cream, the cosmetic composition accounts for 9%, and the conventional cosmetic basic component accounts for 91%. The conventional cosmetic basic component is the same as in Example 2.

[0137] Comparative Example 5

[0138] Different from Example 2, the components of the cosmetic composition are only the hexapeptide composition, the anti-wrinkle oligopeptide composition and the collagen peptide composition:

[0139]

[0140] The remaining parameters were the same as those in Example 2 to prepare a facial cream.

[0141] A method for preparing a cosmetic composition comprises the steps of:

[0142] S1. According to the above weight percentages, the raw materials of each component are mixed and stirred to prepare a hexapeptide composition, an anti-wrinkle oligopeptide composition, and a collagen peptide composition respectively;

[0143] S2. The hexapeptide composition, the anti-wrinkle oligopeptide composition, and the collagen peptide composition are sequentially mixed and stirred for about 45 minutes to obtain a cosmetic composition 12.

[0144] The obtained cosmetic composition is used to prepare a face cream, and the preparation method of the face cream comprises the steps of: taking the above cosmetic composition 12 and mixing it with a conventional cosmetic basic component, stirring at room temperature for about 40 minutes until it is uniform, and obtaining a face cream; in the face cream, the cosmetic composition accounts for 9%, and the conventional cosmetic basic component accounts for 91%. The conventional cosmetic basic component is the same as in Example 2.

[0145] Comparative Example 6

[0146] Different from Example 2, the components of the cosmetic composition are only the anti-wrinkle oligopeptide composition, the collagen peptide composition and the olive essence composition:

[0147]

[0148] The remaining parameters were the same as those in Example 2 to prepare a facial cream.

[0149] A method for preparing a cosmetic composition comprises the steps of:

[0150] S1. According to the above weight percentages, the raw materials of each component are mixed and stirred to prepare an anti-wrinkle oligopeptide composition, a collagen peptide composition, and an olive extract composition respectively;

[0151] S2. The olive extract composition, the anti-wrinkle oligopeptide composition and the collagen peptide composition are sequentially mixed and stirred for about 45 minutes to obtain a cosmetic composition 13.

[0152] The obtained cosmetic composition is used to prepare a face cream, and the preparation method of the face cream comprises the steps of: taking the above cosmetic composition 13 and mixing it with a conventional cosmetic basic component, stirring at room temperature for about 40 minutes until it is uniform, and obtaining a face cream; in the face cream, the cosmetic composition accounts for 9%, and the conventional cosmetic basic component accounts for 91%. The conventional cosmetic basic component is the same as in Example 2.

[0153] Comparative Example 7

[0154] Different from Example 2, no preparation and addition of the cosmetic composition was performed. Only the conventional cosmetic base ingredients of Example 2 were used to obtain a cosmetic cream.

[0155] Comparative Example 8

[0156] The difference from Example 1 is that the collagen peptide composition is replaced by a hexapeptide composition, specifically:

[0157]

[0158] The preparation method of the above-mentioned cosmetic composition comprises the steps of:

[0159] S1. According to the above weight percentages, the raw materials of each component are mixed and stirred to prepare a hexapeptide composition, a skin-tightening tripeptide composition, an anti-wrinkle oligopeptide composition, and an olive extract composition respectively;

[0160] S2, mixing the hexapeptide composition with the olive extract composition, stirring at room temperature for about 15 minutes until the mixture is uniform, to obtain a mixture 1;

[0161] S3. The mixture 1 is sequentially mixed with the skin-tightening tripeptide composition and the anti-wrinkle oligopeptide composition obtained in step S1, and stirred at room temperature for about 40 minutes to obtain a cosmetic composition 15.

[0162] The obtained cosmetic composition is used to prepare essence water, and the preparation method of essence water includes the steps of: taking the above cosmetic composition 15 and mixing it with conventional cosmetic basic ingredients, stirring at room temperature for about 40 minutes until it is uniform, and obtaining essence water; in the essence water, the cosmetic composition accounts for 5%, and the conventional cosmetic basic ingredients account for 95%. The conventional cosmetic basic components are the same as those in Example 1.

[0163] Comparative Example 9

[0164] The difference from Example 1 is that the olive extract composition is replaced by tea polyphenols, and the weight portion of tea polyphenols in the composition is 1 part.

[0165] The cosmetic composition 16 was prepared according to the preparation method, parameters, etc. described in Example 1, and the essence water was prepared.

[0166] Comparative Example 10

[0167] Different from Example 1, the weight parts of the cosmetic composition are different. Specifically, the components in weight parts are: 20 parts of a hexapeptide composition, 1 part of a firming tripeptide composition, 0.1 parts of an anti-wrinkle oligopeptide composition, 0.1 parts of a collagen peptide composition and 1 part of an olive extract composition.

[0168] The rest is the same as Example 1. A cosmetic composition 17 is obtained, and an essence water is prepared.

[0169] Comparative Example 11

[0170] The difference from Example 1 is that the preparation method of the cosmetic composition is different, specifically the temperature and the order of mixing the raw materials are different, and the steps include:

[0171] S1. Prepare a hexapeptide composition, a skin-tightening tripeptide composition, an anti-wrinkle oligopeptide composition, a collagen peptide composition, and an olive extract composition respectively;

[0172] S2, mixing the hexapeptide composition with the skin-tightening tripeptide composition, the anti-wrinkle oligopeptide composition, and the collagen peptide composition in sequence, stirring at 40° C. for about 15 minutes until the mixture is uniform, to obtain a mixture 1;

[0173] S3. Mix the mixture 1 with the olive extract composition obtained in step S1, and stir at 40° C. for about 40 min to obtain a cosmetic composition 18.

[0174] The obtained cosmetic composition is used to prepare essence water. The preparation method of essence water includes the steps of: taking the above cosmetic composition 18 and mixing it with conventional cosmetic basic ingredients, stirring at room temperature for about 40 minutes until it is uniform, to obtain essence water; in the essence water, the cosmetic composition accounts for 5% and the conventional cosmetic basic ingredients account for 95%.

[0175] Effect test 1: wrinkle tightening effect test

[0176] 1. Elastase inhibition rate detection

[0177] By constructing an elastase inhibition mechanism system, the inhibitory effect of the essence water and cream prepared in Examples 1-7 and Comparative Examples 1-11 on elastase was evaluated. Elastase has the ability to degrade collagen, elastin and other proteins. The change in elastin structure during skin aging is one of the important causes of wrinkles and skin sagging. Studies have shown that inhibiting elastase from decomposing elastin can have a firming and anti-wrinkle effect. The anti-wrinkle and firming effects of the essence water and cream prepared in Examples 1-7 and Comparative Examples 1-11 were evaluated in this way.

[0178] The test concentration of the test sample is 40 mg / mL; the test concentration of the positive control: blank substrate is 40 mg / mL.

[0179] Detection method:

[0180] (1) Weigh an appropriate amount of sample into a centrifuge tube and dilute to 10 mL with buffer. Vortex to mix. Perform blank and positive control experiments at the same time.

[0181] (2) Add appropriate amount of elastase solution to the sample tube and background tube respectively, shake well, and let stand at 37℃ for 5 minutes. The reaction is complete;

[0182] (3) Add appropriate amount of substrate solution and DMSO solution, shake well and react at 37℃ for 30 min, and measure the absorbance at 405nm;

[0183] (4) Record the data and calculate the elastase inhibition rate and statistical difference value.

[0184] As a highly selective and specific proteolytic enzyme, elastase catalyzes the hydrolysis of many carboxyl-containing polypeptide bonds and can decompose elastin in connective tissue proteins. The ability of the test sample to inhibit elastase and the statistical difference P value are calculated to determine whether the sample has anti-wrinkle and firming effects.

[0185] Elastase inhibition rate (%) = 1-(A 样品 -B 样品底物 ) / (CD)×100%; where A 样品 Indicates the absorbance of the sample tube, B 样品底物 represents the absorbance of the sample background tube, C represents the absorbance of the solvent tube, and D represents the absorbance of the solvent background tube.

[0186] The results of the inhibition rate test of each essence water and cream on elastase are shown in Table 1:

[0187] Table 1

[0188]

[0189] In Table 1, * indicates that compared with Example 1, 0.01<P<0.05; ** indicates that compared with Example 1, P<0.01.

[0190] It can be seen from the experimental results in Table 1 that the moisturizing lotion and cream prepared in Examples 1-2 of the present invention are significantly better than the essence lotion and cream prepared in Comparative Examples 1-6 in terms of anti-wrinkle and firming effects.

[0191] Moreover, as shown in Table 1 above, Comparative Example 1 only contains the hexapeptide composition and the anti-wrinkle oligopeptide composition, and Comparative Example 3 only contains the hexapeptide composition, the anti-wrinkle oligopeptide composition and the olive extract composition, and the inhibition rate of elastase is the worst.

[0192] The elastase inhibition rates of Comparative Examples 2 and 5-6 were also lower than those of Example 1-2. The present invention combines collagen peptides and olive extract with hexapeptides and oligopeptides to produce a significant synergistic effect in inhibiting elastase.

[0193] Comparative Example 7 shows that conventional cosmetic base ingredients alone for preparing facial creams have substantially no effect of inhibiting elastase.

[0194] 2. Evaluation of human trial effects

[0195] 42 women aged 30-60 were selected as subjects and divided into 7 groups, with 6 people in each group. Before the test, the subjects washed their faces and stayed in a constant temperature and humidity environment (temperature 21-23°C, humidity 55-65%) for 30 minutes, and then took facial photos using C-CUBE as the initial data. The subjects applied the corresponding essence water or cream (Example 1, Example 2, Comparative Example 2, Comparative Example 3, Comparative Example 6, Comparative Example 7 and Comparative Example 8) of each group on their faces, used them twice a day, and tried them for 30 consecutive days. On the 0th day and the 30th day, the facial wrinkle test was performed using C-CUBE after washing the face, which were recorded as D0 and D30 respectively.

[0196] The instrument test data is expressed in SZ values. The Sz value is the wrinkle performance value. A smaller Sz value indicates smoother skin and fewer wrinkles.

[0197] The D0 and D30 values ​​of each group are the average values ​​of these values ​​in the group. The instrument software calculates the improvement of facial wrinkles before and after use. The higher the score, the fewer wrinkles. The test results are shown in Table 2:

[0198] Table 2

[0199]

[0200] From the results in Table 2, it can be seen that the subjects in the comparative example 7 group only used the basic ingredients for preparing conventional cosmetics, and there was basically no wrinkle-removing effect.

[0201] The present invention rationally compounds four peptide compositions with a specific type of olive extract composition, and the obtained cosmetic composition, essence water and face cream produce significant synergistic effects in terms of anti-wrinkle and firming.

[0202] Effect test 2: Repair skin barrier function effect test

[0203] 1. Subject selection

[0204] A total of 56 subjects, 28 males and 28 females, aged 30-50 years old, were randomly divided into 7 groups, with 8 subjects in each group.

[0205] 2. Experimental Materials and Instruments

[0206] The essence water or cream of Example 1, Example 2, Comparative Example 4, Comparative Example 5, Comparative Example 6, Comparative Example 7 and Comparative Example 9;

[0207] 3% SDS (sodium dodecyl sulfate) aqueous solution;

[0208] Hypoallergenic topical adhesive tape (3M Company, USA);

[0209] Mexameter MXI8 red pigment tester (Courage Khazaka Electronic, Germany);

[0210] 3. Experimental methods

[0211] At the preset test area of ​​the forearm of the experimenter, the skin erythema amount was measured with a Mexameter MX18 red pigment tester, and the skin erythema amount at this time was set to 0 (baseline value). 0.02mL 3% SDS solution was accurately measured with a pipette, placed in the center of the spot tester chamber, and then applied to the preset area of ​​the forearm of the volunteer. After applying for 24 hours, the spot tester was removed. The skin erythema amount was detected after 4 hours, and the erythema amount at this time was set to 100%. Then, the same amount of the essence water or cream of Example 1, Example 2, Comparative Example 4, Comparative Example 5, Comparative Example 6, Comparative Example 7 and Comparative Example 9 was applied to the experiment area of ​​the forearm of the experimenter on the 1st to 3rd day after SDS treatment, and the amount of application was 1.2g. The skin erythema amount was measured 4 hours after applying every day.

[0212] 4. Experimental results

[0213] The results of the effect of the cosmetics prepared by the cosmetic composition of the present invention on the amount of skin erythema are shown in Table 3:

[0214] Table 3

[0215]

[0216] From the results in Table 3, it can be seen that since the subjects in the comparative example 7 group used only conventional cosmetic basic ingredients, the relief effect on experimental skin erythema was not obvious during the study period.

[0217] From the results of Comparative Examples 4 and 5, it can be seen that the effect of using the polypeptide composition alone on relieving skin erythema is also poor. Comparative Example 6 uses an anti-wrinkle oligopeptide composition, a collagen peptide composition and an olive extract composition to prepare a cosmetic composition. Although the effect of improving skin erythema is better than that of Comparative Examples 4 and 5, it is still worse than that of Examples 1-2.

[0218] The relative erythema value of the subjects in Example 2 on the 3rd day was reduced from 100% to 19%, which was significantly lower than the relative erythema value of 38% in Comparative Example 6 on the 3rd day. The present invention combines the four peptide compositions and the olive extract composition, which produces a significant synergistic effect in reducing the amount of erythema and repairing the skin barrier function.

[0219] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, rather than to limit the scope of protection of the present invention. Simple modifications or equivalent substitutions of the technical solution of the present invention by ordinary technicians in this field do not deviate from the essence and scope of the technical solution of the present invention.

Claims

1. An anti-wrinkle, firming and repairing cosmetic composition, characterized in that: The components include, by weight: 0.5-15 parts of a hexapeptide composition, 0.5-5 parts of a skin-tightening tripeptide composition, 0.5-5 parts of an anti-wrinkle oligopeptide composition, 0.5-5 parts of a collagen peptide composition, and 0.1-2 parts of an olive extract composition; The hexapeptide composition comprises the following components by weight percentage: 0.03-1% acetyl hexapeptide-8, 0.1-1% caprylyl glycol and the balance water; the skin-tightening tripeptide composition comprises the following components by weight percentage: 0.01-0.05% trifluoroacetyl tripeptide-2, 0.05-0.1% dextran, 60-80% glycerol and the balance water; the anti-wrinkle oligopeptide composition comprises the following components by weight percentage: 1-10% oligopeptide-1, 1-10% hexanoic acid, 1-10% dextran, 20-80% glycerol and the balance water; the collagen peptide composition comprises the following components by weight percentage: 0.5-2% palmitoyl tripeptide-5, 50-60% glycerol and the balance water; the olive extract composition comprises the following components by weight percentage: 5-10% olive leaf extract, 50-70% fructose and the balance water.

2. The cosmetic composition according to claim 1, characterized in that The components include, by weight: 0.8-10 parts of a hexapeptide composition, 0.8-3 parts of a skin-tightening tripeptide composition, 0.8-3 parts of an anti-wrinkle oligopeptide composition, 0.8-3 parts of a collagen peptide composition and 0.5-1.5 parts of an olive extract composition.

3. The cosmetic composition according to claim 2, characterized in that The components include, by weight: 1-5 parts of a hexapeptide composition, 1-3 parts of a skin-tightening tripeptide composition, 1-3 parts of an anti-wrinkle oligopeptide composition, 0.8-2 parts of a collagen peptide composition and 0.8-1.2 parts of an olive extract composition.

4. The cosmetic composition according to any one of claims 1 to 3, characterized in that The hexapeptide composition comprises the following components by weight percentage: 0.045-0.055% acetyl hexapeptide-8, 0.45-0.55% caprylyl glycol and the balance water.

5. The cosmetic composition according to any one of claims 1 to 3, characterized in that The hexapeptide composition comprises the following components by weight percentage: 0.05% acetyl hexapeptide-8, 0.5% caprylyl glycol and the balance water; the skin-tightening tripeptide composition comprises the following components by weight percentage: 0.025% trifluoroacetyl tripeptide-2, 0.08% dextran, 65% glycerol and the balance water; the anti-wrinkle oligopeptide composition comprises the following components by weight percentage: 5% oligopeptide-1, 5% hexanoic acid, 5% dextran, 60% glycerol and the balance water; the collagen peptide composition comprises the following components by weight percentage: 1% palmitoyl tripeptide-5, 51% glycerol and the balance water; the olive extract composition comprises the following components by weight percentage: 7.5% olive leaf extract, 60% fructose and the balance water.

6. The method for preparing the cosmetic composition according to any one of claims 1 to 5, characterized in that: Includes steps: S1. Mix the raw materials of each component according to weight percentage, stir, and respectively prepare a hexapeptide composition, a skin-tightening tripeptide composition, an anti-wrinkle oligopeptide composition, a collagen peptide composition, and an olive extract composition; S2, mixing the hexapeptide composition with the olive extract composition, stirring to obtain a mixture 1; S3. Mix the mixture 1 with the skin-tightening tripeptide composition, the anti-wrinkle oligopeptide composition, and the collagen peptide composition to obtain a cosmetic composition.

7. Use of a cosmetic composition in the preparation of cosmetics with all-round anti-wrinkle, firming, delicate skin and repairing effects, characterized in that: The cosmetic composition is the cosmetic composition according to any one of claims 1 to 5 or the cosmetic composition prepared by the preparation method according to claim 6.

8. Use of a cosmetic composition in the preparation of cosmetics having the effect of preventing or improving sagging, roughness and aging, characterized in that: The cosmetic composition is the cosmetic composition according to any one of claims 1 to 5 or the cosmetic composition prepared by the preparation method according to claim 6.

9. A cosmetic comprising the cosmetic composition according to any one of claims 1 to 5 and conventional cosmetic basic ingredients.

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