A multi-effect composite protein composition and its preparation method and application

Abstract

The present application relates to the field of A61K8 / 18, in particular to a kind of composite protein composition with multiple effects and its preparation method and application, and its preparation raw material at least includes the following ingredients according to weight part: natural protein 10-30 parts, N-vinylpyrrolidone 1-2 parts, hydrogen peroxide aqueous solution 8-12 parts, horseradish peroxidase aqueous solution 8-12 parts, deionized water 150-220 parts, the composition overcomes the defect that traditional clothes class product single effect cannot satisfy consumer's demand, effectively improves fabric damage, keeps the integrity of fabric fiber, avoids the skin-friendly problem of fabric after washing effect complete fabric, while the composition has higher compatibility, can be added and applied in clothes washing and care product.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of A61K8 / 18, in particular to a composite protein composition with multiple effects and its preparation method and application. BACKGROUND

[0002] With the gradual improvement of people's living standards, the standard of wearing clothes is also improved, and there is a greater demand for delicate care of some expensive and delicate clothes. The existing clothes care products mainly have single functions such as color protection, anti-static, softness and ordinary cleaning function. The current fabric care technology realizes a single function, and realizes a single function such as color protection, anti-static, softness through one or several functional ingredients. There is a limitation in function and a problem that the function is not fully realized in the care of clothes. For such consumers who demand delicate care of clothes, they hope that there will be a multi-effect clothes care product. The current market products have single effect but are not comprehensive and have limited effect, and cannot realize effective clothes care of multiple functions. For example, Chinese patent application CN 102333853A discloses a composition for washing and then drying delicate clothes without causing any damage and its use method. In the disclosed patent, water and certain additives are used instead of organic solvents to realize the cleaning effect of the clothes while avoiding size change, dye or color loss. However, the composition is mainly for washing, and cannot comprehensively care for expensive and delicate clothes. In addition, after the fabric is rubbed for many times, the surface fibers of the fabric will inevitably be damaged to different degrees, and there is a problem of skin-friendliness of the fabric after washing. SUMMARY

[0003] In order to overcome the defect that the single effect of the traditional clothes care product cannot meet the needs of consumers, a composite protein composition with multiple effects is provided, which effectively improves the damage of the fabric, maintains the integrity of the fabric fibers, avoids the skin-friendliness problem of the fabric after washing, and at the same time, the composition has high compatibility and can be added to the clothes washing and care products.

[0004] In one aspect, the present application provides a composite protein composition with multiple effects, which is prepared from the following raw materials in parts by weight: 10-30 parts of natural protein, 1-6 parts of N-vinyl pyrrolidone, 8-12 parts of hydrogen peroxide aqueous solution, 8-12 parts of horseradish peroxidase aqueous solution, and 150-220 parts of deionized water.

[0005] As a preferred technical solution, the natural protein is one or a combination of silk protein, wheat protein, cashmere keratin, cottonseed protein, sericin, corn protein and peanut protein.

[0006] As a preferred technical solution, the N-vinyl pyrrolidone is 10 g / L-200 g / L N-vinyl pyrrolidone aqueous solution. Preferably, the N-vinyl pyrrolidone is 20 g / L-120 g / L N-vinyl pyrrolidone aqueous solution.

[0007] Based on the system of the application, by controlling the introduction of 20 g / L-120 g / L N-vinyl pyrrolidone aqueous solution, and controlling the mass ratio of natural protein and N-vinyl pyrrolidone to be (10-30):(1.5-5), the natural protein and the N-vinyl pyrrolidone aqueous solution at this concentration form a copolymer, so that the natural protein is effectively attached and fixed to the surface of the fabric, forming a protective film on the fabric to avoid the skin-friendly problem of the fabric after washing. At the same time, various natural proteins work synergistically to achieve the effects of color protection, antistatic, softening, air permeability and fiber protection. The inventor analyzes the reason: under the processing conditions of the system of the application, 20 g / L-120 g / L N-vinyl pyrrolidone aqueous solution and natural protein form a hydrolyzed protein and a polyvinyl pyrrolidone copolymer, making the surface of the clothes smooth and maintaining its elasticity. The silk protein in the system has a moisturizing effect, good hydrophilic force and strong penetration force, which can lock water in the fabric fibers; the wheat protein has ductility and elasticity, and can form a network structure with water, thereby having excellent viscoelasticity, extensibility and water absorption, making the fabric fibers more elastic and soft; the cashmere keratin has strong elongation and good moisture absorption, making the fabric skin-friendly and smooth, with delicate touch and good antibacterial effect; the cottonseed protein has excellent antistatic, dispersing, emulsifying, wetting and penetrating ability, making the fabric antistatic, skin-friendly and breathable; the sericin has similar natural moisture regulating factors in the human skin keratin layer, has the effect of inhibiting tyrosinase activity, and can make the fabric more skin-friendly and resistant to fabric oxidation; the corn protein has antistatic property and is an antioxidant and emollient, making the fabric antistatic and preventing oxidation. The peanut protein has clear antioxidant effect on free radicals. Under the action of N-vinyl pyrrolidone, the natural protein is effectively attached to the fabric fibers, so that the natural protein can fully exert the synergistic effect, overcoming the defect that traditional fabric care products have single function and cannot meet the needs of consumers.

[0008] As a preferred technical solution, the concentration of the hydrogen peroxide aqueous solution is 0.01-10 g / L.

[0009] As a preferred technical solution, the concentration of the horseradish peroxidase aqueous solution is 0.01-5 g / L.

[0010] Another aspect of the present application provides a preparation method of a composite protein composition with multiple effects, at least comprising the following steps:

[0011] (1) According to the weight parts, deionized water is added to a water bath stirring pot, and the temperature is raised to 35-38℃ to form phase A;

[0012] (2) After adding natural protein to phase A, hydrolytic enzyme is added, and the hydrolysis reaction is carried out after sufficient stirring. After the reaction is completed, the temperature is raised to 68-72℃, and stirring is carried out for 20-40 min. After cooling to room temperature, the hydrolyzed protein solution is obtained by filtration;

[0013] (3) According to the weight parts, N-vinyl pyrrolidone is added to a water bath stirring pot to form phase B;

[0014] (4) Hydrogen peroxide aqueous solution and horseradish peroxidase aqueous solution are added to phase B, and stirring and mixing are carried out for 15-30 min to obtain an N-vinyl pyrrolidone mixed solution;

[0015] (5) The hydrolyzed protein solution is added to the N-vinyl pyrrolidone mixed solution under stirring, and the temperature is slowly raised to 70-80℃. After sufficient reaction, a copolymer solution C phase is obtained;

[0016] (6) The temperature is controlled at 80-100℃, and the copolymer solution C phase is subjected to rotary evaporation under reduced pressure. After dehydration, it is obtained.

[0017] As a preferred technical solution, the hydrolysis reaction time in step (2) is 1.5-2.5 h; the natural protein is hydrolyzed in water at 35-38℃ for 1.5-2.5 h to obtain hydrolyzed protein, and the temperature is raised to inactivate the residual enzyme, so as to avoid the influence of residual enzyme on the subsequent polymerization reaction and the compatibility of the formula in actual application.

[0018] As a preferred technical solution, the reaction time in step (5) is 4-6 h; based on the system of the present application, the N-vinyl pyrrolidone and the hydrolyzed protein are polymerized at a temperature of 70-80℃ in the presence of an initiator for 4-6 h to obtain a hydrolyzed protein and polyvinyl pyrrolidone copolymer. The multiple hydrolyzed proteins are effectively fixed, so that the provided composition can be effectively added to laundry care products to improve fabric damage and maintain the integrity of fabric fibers.

[0019] The application of a composite protein composition with multiple effects is applied to the addition of laundry care products. Preferably, the amount of the composition added to the laundry care products is 0.1-10 wt%.

[0020] Advantages:

[0021] 1. The present application provides a kind of composite protein composition with multiple effects, overcome the single effect of traditional protective clothing product cannot meet the needs of consumers defects, effectively improve the damage of fabric, keep the integrity of fabric fiber, avoid the skin problem of fabric after washing, and the composition has high compatibility, can be added and applied in laundry care products.

[0022] 2. Based on the system of the present application, by controlling the introduction of 20g / L-120g / L N-vinyl pyrrolidone aqueous solution, and controlling the mass ratio of natural protein and N-vinyl pyrrolidone to be (10-30):(1.5-5), the natural protein and the N-vinyl pyrrolidone aqueous solution of the concentration form a copolymer, so that the natural protein is effectively attached and fixed on the surface of the fabric, a protective film is formed on the fabric to avoid the skin problem of the fabric after washing, and various natural proteins play a synergistic role, realizing the effects of color protection, antistatic, softening, air permeability and fiber protection on the fiber.

[0023] 3. Based on the system of the present application, by controlling the natural protein to be hydrolyzed in water at 35-38℃ for 1.5-2.5h, the natural protein is fully hydrolyzed to obtain hydrolyzed protein, and then the temperature is raised to inactivate the residual enzyme, avoiding the influence of enzyme residue on subsequent polymerization reaction and the compatibility of formula matching in actual application.

[0024] 4. Based on the system of the present application, by controlling the temperature to be 70-80℃, N-vinyl pyrrolidone and hydrolyzed protein are polymerized for 4-6h in the presence of initiator to obtain hydrolyzed protein and polyvinyl pyrrolidone copolymer, effectively fixing a variety of hydrolyzed proteins, so that the provided composition can be effectively added and applied in laundry care products, improving the damage of fabric and keeping the integrity of fabric fiber. DETAILED DESCRIPTION

[0025] Example 1

[0026] Embodiment 1 of the present application provides a composite protein composition with multiple effects and a preparation method thereof, 180g of deionized water is added to a three-necked flask, and heated and stirred to 35℃, 20g of natural protein is added, and then a hydrolytic enzyme is added, and the hydrolysis is fully stirred, for 2h, after the reaction is completed, the temperature is increased to 70℃ for reaction and stirring, for 30min, so as to inactivate the residual enzyme, and then the temperature is decreased to 25℃, and the hydrolyzed protein solution is obtained by filtration; in another three-necked flask, 500ml of 30g / L N-vinyl pyrrolidone aqueous solution is added; 10ml of 1g / L hydrogen peroxide aqueous solution and 10ml of 0.5g / L horseradish peroxidase aqueous solution are added into the N-vinyl pyrrolidone aqueous solution, and fully stirred and mixed; then the hydrolyzed protein solution is added, and slowly heated to 80℃, and fully stirred and reacted at 80℃ for 5h, so as to make the hydrolyzed protein and the polyvinyl pyrrolidone react into a copolymer; vacuum rotary evaporation is performed, and water is removed at 100℃ until no liquid is discharged, and the composition is obtained.

[0027] The natural protein includes 5g of wheat protein, 5g of cashmere keratin, 5g of cottonseed protein and 5g of peanut protein.

[0028] The application of the composite protein composition with multiple effects is applied to the addition of laundry care products, and the addition amount of the composition applied to the laundry care products is 2wt%.

[0029] Embodiment 2

[0030] Embodiment 2 of the present application provides a composite protein composition with multiple effects and a preparation method thereof, 180g of deionized water is added to a three-necked flask, and heated and stirred to 35℃, 20g of natural protein is added, and then a hydrolytic enzyme is added, and the hydrolysis is fully stirred, for 2h, after the reaction is completed, the temperature is increased to 70℃ for reaction and stirring, for 30min, so as to inactivate the residual enzyme, and then the temperature is decreased to 25℃, and the hydrolyzed protein solution is obtained by filtration; in another three-necked flask, 500ml of 30g / L N-vinyl pyrrolidone aqueous solution is added; 10ml of 1g / L hydrogen peroxide aqueous solution and 10ml of 0.5g / L horseradish peroxidase aqueous solution are added into the N-vinyl pyrrolidone aqueous solution, and fully stirred and mixed; then the hydrolyzed protein solution is added, and slowly heated to 80℃, and fully stirred and reacted at 80℃ for 5h, so as to make the hydrolyzed protein and the polyvinyl pyrrolidone react into a copolymer; vacuum rotary evaporation is performed, and water is removed at 100℃ until no liquid is discharged, and the composition is obtained.

[0031] The natural protein includes 5g of silk protein, 5g of cashmere keratin, 5g of peanut protein and 5g of sericin protein.

[0032] The application of the composite protein composition with multiple effects is applied to the addition of laundry care products, and the addition amount of the composition applied to the laundry care products is 2wt%.

[0033] Example 3

[0034] Example 3 of the present application provides a composite protein composition with multiple effects and a preparation method thereof, 180 g of deionized water is added to a three-necked flask, and the temperature is raised to 35°C while stirring, 20 g of natural protein is added, and then a hydrolysis enzyme is added, and the mixture is stirred and hydrolyzed for 2 h, after the reaction is completed, the temperature is raised to 70°C for reaction and stirring for 30 min to inactivate the residual enzyme, and then the temperature is lowered to 25°C, and the hydrolyzed protein solution is obtained by filtration; in another three-necked flask, 500 ml of 100 g / L N-vinyl pyrrolidone aqueous solution is added, 10 ml of 3 g / L hydrogen peroxide aqueous solution and 10 ml of 2 g / L horseradish peroxidase aqueous solution are added to the N-vinyl pyrrolidone aqueous solution, and the mixture is stirred and mixed uniformly; then the hydrolyzed protein solution is added, and the temperature is slowly raised to 80°C, and the mixture is stirred and reacted at 80°C for 5 h to make the hydrolyzed protein and polyvinylpyrrolidone react into a copolymer; vacuum rotary evaporation is performed at 100°C to remove water until no liquid is discharged, and the composition is obtained.

[0035] The natural protein includes 5 g of wheat protein, 5 g of silk protein, 5 g of peanut protein, and 5 g of sericin.

[0036] The application of the composite protein composition with multiple effects is applied to the addition of laundry care products. The addition amount of the composition in the laundry care product is 1.2 wt%.

[0037] Performance test method

[0038] The composite protein composition with multiple effects prepared in Example 1 is added to prepare a laundry care product, and the formula of the laundry care product is shown in the following table, and the fabric softening effect test and stability of the laundry care product are determined.

[0039]

[0040] 1. Stability

[0041] (1) Low-temperature stability: laundry liquids A and B are placed in a refrigerator at (-5±2) °C for 24 hours, and then taken out and observed after being restored to room temperature. No odor, no precipitation, and no discoloration are observed for laundry liquids A and B.

[0042] (2) High-temperature stability: laundry liquids A and B are placed in an oven at (40±2) °C for 24 hours, and then immediately observed. No odor, no precipitation, and no discoloration are observed for laundry liquids A and B.

[0043] 2. Fabric softening effect test

[0044] (1) Purpose: Use laundry method to wash fabric, and compare fabric softening effect.

[0045] (2) Test equipment: laundry detergent A, laundry detergent B, drum washing machine, same towels.

[0046] (3) Test steps: In the use site, 3 groups of 5 same towels are processed by washing machine in fast washing mode.

[0047] 1) No laundry detergent is added for fast washing 15min mode, repeated 5 times, and naturally dried, which is the blank control group.

[0048] 2) Add laundry detergent A 10ml each time for fast washing 15min mode, repeated 5 times, and naturally dried, which is experimental group 1.

[0049] 3) Add laundry detergent B 10ml each time for fast washing 15min mode, repeated 5 times, and naturally dried, which is experimental group 2.

[0050] 4) After the test, the towels in each group are tested by personnel sensory test and scored, and the results are observed.

[0051] (4) Test results

[0052] The softness of the towel is evaluated by sensory evaluation with softness degree score from 0 to 5. The following is the scoring record of 20 test personnel, and the results are shown in Table 1.

[0053] Table 1,

[0054]

[0055] 3, Friction test: According to FZ / T 01054-2012 Test method for fabric surface friction performance, the static and dynamic friction coefficients of the washed towels are tested under dry friction condition, and the results are shown in Table 2.

[0056] 1, Treatment condition

[0057] 1) No laundry detergent is added for fast washing 15min mode, repeated 5 times, and naturally dried, which is the blank control group.

[0058] 2) Add laundry detergent A 10ml each time for fast washing 15min mode, repeated 5 times, and naturally dried, which is experimental group 1.

[0059] 3) Add laundry detergent B 10ml each time for fast washing 15min mode, repeated 5 times, and naturally dried, which is experimental group 2.

[0060] 2, Test results are shown in Table 2.

[0061] Table 2,

[0062] Control group Experimental group 1 Experimental group 2 coefficient of static friction μ s ]] 0.537 0.312 0.443 coefficient of kinetic friction μ k ]] 0.489 0.283 0.418

[0063] Note: The greater the coefficient of friction, the greater the friction between the surfaces of the objects, indicating that the surface of the fabric is rougher and less supple.

Claims

1. A multi-functional complex protein composition, characterized in that, The raw materials for its preparation include at least the following components by weight: 10-30 parts of natural protein, 1-6 parts of N-vinylpyrrolidone, 8-12 parts of hydrogen peroxide aqueous solution, 8-12 parts of horseradish peroxidase aqueous solution, and 150-220 parts of deionized water. The natural protein is a combination of four of the following: silk protein, wheat protein, cashmere keratin, cottonseed protein, sericin, corn protein, and peanut protein. The N-vinylpyrrolidone is an aqueous solution of N-vinylpyrrolidone with a concentration of 20 g / L to 120 g / L; The preparation method of the aforementioned composite protein composition includes the following steps: (1) Add deionized water to a water bath stirring pot according to the weight parts and heat it to 35~38℃ to form phase A; (2) After adding natural protein to phase A, add hydrolytic enzyme, stir thoroughly to carry out hydrolysis reaction. After the reaction is completed, heat to 68-72℃ and stir for 20-40 minutes. Cool down to room temperature and filter to obtain hydrolyzed protein solution. (3) Add N-vinylpyrrolidone to a water bath stirring vessel according to the weight proportions to form phase B; (4) Add hydrogen peroxide aqueous solution and horseradish peroxidase aqueous solution to phase B, stir and mix for 15-30 min to obtain N-vinylpyrrolidone mixed solution; (5) Add hydrolyzed protein solution to N-vinylpyrrolidone mixed solution under stirring, slowly heat to 70-80℃, and after sufficient reaction, obtain copolymer solution C phase; (6) Control the temperature to 80-100℃, and perform vacuum rotary evaporation on the C phase of the copolymer solution to remove water.

2. The multi-functional complex protein composition according to claim 1, characterized in that, The concentration of the hydrogen peroxide aqueous solution is 0.01~10 g / L.

3. The application of the multi-functional complex protein composition according to claim 2, characterized in that, The concentration of the horseradish peroxidase aqueous solution is 0.01~5 g / L.

4. A method for preparing a multi-functional complex protein composition according to any one of claims 1-3, characterized in that, At least the following steps are included: (1) Add deionized water to a water bath stirring pot according to the weight parts and heat it to 35~38℃ to form phase A; (2) After adding natural protein to phase A, add hydrolytic enzyme, stir thoroughly to carry out hydrolysis reaction. After the reaction is completed, heat to 68-72℃ and stir for 20-40 minutes. Cool down to room temperature and filter to obtain hydrolyzed protein solution. (3) Add N-vinylpyrrolidone to a water bath stirring vessel according to the weight proportions to form phase B; (4) Add hydrogen peroxide aqueous solution and horseradish peroxidase aqueous solution to phase B, stir and mix for 15-30 min to obtain N-vinylpyrrolidone mixed solution; (5) Add hydrolyzed protein solution to N-vinylpyrrolidone mixed solution under stirring, slowly heat to 70-80℃, and after sufficient reaction, obtain copolymer solution C phase; (6) Control the temperature to 80-100℃, and perform vacuum rotary evaporation on the C phase of the copolymer solution to remove water.

5. The method for preparing a multi-functional complex protein composition according to claim 4, characterized in that, The hydrolysis reaction time in step (2) is 1.5~2.5h; the reaction time in step (5) is 4~6h.

6. The application of a multifunctional complex protein composition according to any one of claims 1-3, characterized in that, It can be used as an additive in laundry and care products.

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