A method for identifying flos farinae by thin-layer chromatography

Abstract

The present application relates to a kind of thin layer chromatography identification method of honeycomb medicinal materials, belong to the technical field of quality control of traditional Chinese medicine, the present application is with honeycomb control medicinal materials as control to the thin layer chromatography identification of honeycomb medicinal materials.The present application supplements the deficiency of the quality control method of honeycomb medicinal materials in prior art, the thin layer chromatography identification method established is simple, chromatographic spot is clear, strong in specificity, good reproducibility, good universality, can better control the quality of honeycomb medicinal materials, and has good application value to the quality control of other honeycomb-containing traditional Chinese medicine preparations.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of quality control of traditional Chinese medicine, and relates to a qualitative identification method of Flos Bombusae, in particular to a thin layer chromatography identification method of Flos Bombusae. BACKGROUND

[0002] Flos Bombusae is the nest of Polistes olivaceous (DeGeer), Polistes japonicas Saussure or Parapolybia varia Fabricius of the family Vespidae, is sweet, flat, and belongs to the stomach channel, has the functions of attacking toxin and killing insects, dispelling wind and relieving pain, and is used for treating sore, mastitis, scrofula, tinea manuum, tinea pedis, toothache, rheumatic arthralgia, etc., is one of the commonly used traditional Chinese medicines, and is recorded in many versions of Chinese Pharmacopoeia. Flos Bombusae is a high-priced traditional Chinese medicinal material, and the qualities of Flos Bombusae on the market are mixed, and the prices can differ by tens of times, so it is particularly important to ensure the quality of the medicinal material.

[0003] Good quality of medicinal materials is the basis and premise of playing a clinical curative effect, and qualitative identification is an important link of identifying the authenticity of medicines and ensuring the quality of medicines. Moreover, most modern traditional Chinese medicine preparations have been extracted and purified, and most medicinal materials have lost their properties and microscopic characteristics, so that the quality control of medicines is brought certain difficulty.

[0004] Medicines (including traditional Chinese medicinal materials and decoction pieces) should meet the national medicine standards, and Chinese Pharmacopoeia is an important part and a summary of the national medicine standards, is a statutory technical standard that should be followed by relevant units such as medicine research, production (import), operation, use and supervision and management. Chinese Pharmacopoeia is not only one of the effective tools for medicine quality control and public health protection, but also plays an important role in standardizing industry order and guiding industry development. Thin layer chromatography has the advantages of simple equipment, convenient operation, rapidness, multiple color development modes, high sensitivity (compared with paper chromatography), and is particularly suitable for identification of traditional Chinese medicinal materials and preparations. In the current version of Chinese Pharmacopoeia (2020 edition), more than 2700 kinds of traditional Chinese medicinal materials, decoction pieces, extracts and prepared formulations are recorded, and thin layer chromatography identification methods are specified for almost all traditional Chinese medicinal materials and all Chinese patent medicines.

[0005] However, so far, there is no general thin layer chromatography identification method suitable for honeycomb. The current edition of Chinese Pharmacopoeia, the honeycomb medicinal material standard, only stipulates the identification of the appearance, and there is no thin layer chromatography identification. The thin layer chromatography identification of honeycomb is not provided in the standards of traditional Chinese medicinal materials and decoction pieces in various editions of various provinces. Not only does it cause the imperfection of the honeycomb medicinal material standard, but also it loses the guidance for the identification of other traditional Chinese medicine preparations containing honeycomb. For example, the traditional Chinese medicine preparations containing honeycomb on the market, such as musk rheumatism capsule (tablet), turtle shell decoction pill, and nitro yellow dampness powder, do not have the thin layer chromatography identification of honeycomb, which is undoubtedly a kind of defect for the quality control.

[0006] In the prior art, there is a lack of a honeycomb medicinal material thin layer chromatography identification method which is simple, specific, reproducible and universal. SUMMARY

[0007] In view of the deficiencies of the prior art, the purpose of the present application is to provide a honeycomb medicinal material thin layer chromatography identification method, which is characterized by using honeycomb reference medicinal material as a reference. The method is simple, specific, reproducible and universal, and can be used for the thin layer chromatography identification and quality control of honeycomb medicinal materials.

[0008] In order to achieve the above-mentioned purpose, the specific scheme adopted by the present application is as follows:

[0009] A honeycomb medicinal material thin layer chromatography identification method, comprising the following steps:

[0010] Step one, preparation of test solution: take honeycomb medicinal material, grind it, take 0.2-1.0 g, add 10-50 ml of diethyl ether, soak the liquid, filter, evaporate the filtrate, add 1 ml of ethyl acetate to the residue to dissolve it as the test solution;

[0011] Step two, preparation of reference medicinal material solution: take 0.2-1.0 g of honeycomb reference medicinal material, add 10-50 ml of diethyl ether, soak the liquid, filter, evaporate the filtrate, add 1 ml of ethyl acetate to the residue to dissolve it as the reference medicinal material solution;

[0012] Step three, identification method: take 0.5-10 μl of the test solution prepared in step one and the reference medicinal material solution prepared in step two, respectively, and point them on the same silica gel G thin layer plate, use n-hexane-ethyl acetate as the developing agent, develop, take out, dry, and observe under a 365 nm ultraviolet light; in the test chromatogram, the same color fluorescent spots appear at the positions corresponding to the reference medicinal material chromatogram.

[0013] Preferably, in step three, the loading amount is 1-5 μl.

[0014] Preferably, in step three, the volume ratio of n-hexane to ethyl acetate in the developing agent is 19-2:1.

[0015] Preferably, in the developing agent, a polar similar organic solvent is used instead of n-hexane. Further, the polar similar organic solvent is cyclohexane or petroleum ether of 60-90℃.

[0016] Preferably, the thin layer chromatography identification method comprises the following steps:

[0017] Step one, preparation of test solution: take honeycomb medicinal materials, grind finely, take 0.5 g, add 30 ml of diethyl ether, soak the liquid, filter, evaporate the filtrate to dryness, add 1 ml of ethyl acetate to dissolve as the test solution;

[0018] Step two, preparation of control drug solution: take 0.5 g of honeycomb control drug, add 30 ml of diethyl ether, soak the liquid, filter, evaporate the filtrate to dryness, add 1 ml of ethyl acetate to dissolve as the control drug solution;

[0019] Step three, identification method: take 2 μl of the test solution prepared in step one and the control drug solution prepared in step two respectively, and point them on the same silica gel G thin layer plate, use n-hexane-ethyl acetate with a volume ratio of 4:1 as the developing agent, develop, take out, dry, and observe under a 365 nm ultraviolet light; in the test chromatogram, the same color fluorescent spots appear at the positions corresponding to the control drug chromatogram.

[0020] Beneficial effects: the thin layer chromatography identification method of honeycomb medicinal materials provided by the present application supplements the shortcomings of the quality control method of honeycomb medicinal materials, and the established thin layer chromatography identification method is simple, the chromatographic spots are clear, the specificity is strong, the reproducibility is good, the universality is good, the quality of honeycomb medicinal materials can be better controlled, and it also has good application value for the quality control of other honeycomb-containing traditional Chinese medicine preparations. BRIEF DESCRIPTION OF DRAWINGS

[0021] Figure 1 is the thin layer chromatogram of 14 batches of honeycomb test products.

[0022] Figure 1 In the figure, 1. honeycomb (Henan Wanguo Pharmacy), 2. honeycomb (Henan Luoyang Orthopedic Hospital), 3. honeycomb (Zhang Zhongjing Pharmacy Hengda Store), 4. honeycomb (Gaoji-Baizhao Health Medicine Old City Store), 5. honeycomb (Lianhe Pharmacy), 6. honeycomb (Qidong Pharmacy), 7. honeycomb (Jingdong Health Alliance Pharmacy-Wanjia Kang Pharmacy Shuanghe Store), S. honeycomb control drug, 8. honeycomb (Tongxin Pharmacy Old City Store), 9. honeycomb (Kaisenren Pharmacy Hengda Store), 10. honeycomb (Dingxin Chain Oasis Pharmacy), 11. honeycomb (People's Pharmacy), 12. honeycomb (Kirin Pharmacy), 13. honeycomb (Weikang Pharmacy), 14. honeycomb (Daxianlin Pharmacy-Baizhetang Pharmacy Qiming South Road Store).

[0023] Figure 2 is the developing agent: n-hexane-ethyl acetate (19:1) developing effect diagram.

[0024] Figure 3 is the developing agent: n-hexane-ethyl acetate (9:1) developing effect diagram.

[0025] Figure 4 is the developing agent: n-hexane-ethyl acetate (3:1) developing effect diagram.

[0026] Figure 5 is the developing agent: n-hexane-ethyl acetate (2:1) developing effect diagram.

[0027] The above Figures 2-5 1. Honeycomb medicinal materials (Luoyang orthopedic hospital in Henan Province), 2. Honeycomb medicinal materials (Dingxin chain - Oasis large pharmacy), 3. Honeycomb reference medicinal materials, 4. Honeycomb medicinal materials (Wanguo large pharmacy in Henan), 5. Honeycomb medicinal materials (Kirin large pharmacy).

[0028] Figure 6 is the developing effect diagram of different sample loading amounts.

[0029] Figure 6 1. Honeycomb reference medicinal materials (0.5 μl), 2. Honeycomb reference medicinal materials (1 μl), 3. Honeycomb reference medicinal materials (2 μl), 4. Honeycomb reference medicinal materials (4 μl), 5. Honeycomb reference medicinal materials (5 μl), 6. Honeycomb reference medicinal materials (10 μl).

[0030] Figure 7 is the developing effect diagram of Qingdao Marine Chemical Co., Ltd. silica gel G thin layer plate (batch number 20211028).

[0031] Figure 7 1. Honeycomb medicinal materials (Luoyang orthopedic hospital in Henan Province), 2. Honeycomb reference medicinal materials, 3. Honeycomb medicinal materials (Dingxin chain - Oasis large pharmacy), 4. Honeycomb medicinal materials (Kirin large pharmacy).

[0032] Figure 8 is the developing effect diagram of Qingdao Hailang Silica Gel Drier Co., Ltd. silica gel G thin layer plate (batch number 20201101).

[0033] Figure 9 is the developing effect diagram of Yantai City Chemical Industry Research Institute silica gel G thin layer plate (batch number 20211025).

[0034] Figure 10 is the developing agent: cyclohexane-ethyl acetate (4:1) developing effect diagram.

[0035] Figure 11 is the developing agent: petroleum ether (60-90℃)-ethyl acetate (4:1) developing effect diagram.

[0036] Figure 1 is a TLC chromatogram of the bee hive medicine in the above-mentioned Figures 8-11 Figure 1 is a TLC chromatogram of the bee hive medicine in the above-mentioned

[0037] Figure 12 Figure 1 is a TLC chromatogram of the bee hive medicine in the above-mentioned

[0038] Figure 12 Figure 1 is a TLC chromatogram of the bee hive medicine in the above-mentioned

[0039] Figure 13 Figure 1 is a TLC chromatogram of the bee hive medicine in the above-mentioned

[0040] Figure 13 Figure 1 is a TLC chromatogram of the bee hive medicine in the above-mentioned

[0041] Figure 14 Figure 1 is a TLC chromatogram of the bee hive medicine in the above-mentioned

[0042] Figure 14 Figure 1 is a TLC chromatogram of the bee hive medicine in the above-mentioned DETAILED DESCRIPTION

[0043] The technical solutions in the embodiments of the present application will be clearly and completely described below in combination with the embodiments of the present application.

[0044] I. Test materials

[0045] Honeycomb, 14 samples were purchased from each pharmacy and hospital: Henan Wanguo Pharmacy, Luoyang Orthopedic Hospital in Henan Province, Zhang Zhongjing Pharmacy Hengda Store, Gaoji-Baijia Good Doctor Medicine Old City Store, Lianyi Pharmacy, Qidong Pharmacy, Jingdong Health Alliance Pharmacy-Wanjia Kang Pharmacy Henghe Store, Tongxin Pharmacy Old City Store, Kaixinren Pharmacy Hengda Store, Dingxin Chain Oasis Pharmacy, People's Pharmacy, Kirin Pharmacy, Weikang Pharmacy, Daxianlin Pharmacy-Baizitang Pharmacy Qiming South Road Store; Honeycomb control medicinal materials were purchased from China Institute for Food and Drug Control (Batch No.: 121265-201502); Moschus rheumatism capsules, Chongqing Tongjunge Pharmaceutical Co., Ltd. of Taiji Group (Batch No.: 21110001, 21110002, 21110003); Biejia Decoction Pills, China National United Pharmaceutical Industry Co., Ltd. (Batch No.: 221488, 221489, 221490); Nitro yellow dampness powder, Luoyang Orthopedic Hospital in Henan Province (Batch No.: 20180401, 20180402, 20180403).

[0046] Silica gel G thin layer plate was purchased from Qingdao Haoyang Chemical Co., Ltd. (Batch No.: 20211028), Qingdao Haiwang Silica Gel Dryer Co., Ltd. (Batch No.: 20201101), Yantai Chemical Industry Research Institute (Batch No.: 20211025); all reagents used were analytical pure.

[0047] II. Establishing a thin layer chromatography identification method for honeycomb medicinal materials with honeycomb control medicinal materials as control

[0048] (1) Thin layer chromatography identification of honeycomb medicinal materials from multiple sources and multiple samples

[0049] Take 14 samples of honeycomb from the market randomly purchased from different sources, grind them, take 0.5 g, add 30 ml of ethyl ether, soak the liquid, filter, evaporate the filtrate to dryness, add 1 ml of ethyl acetate to dissolve the residue as the test solution. Another 0.5 g of honeycomb control medicinal materials was prepared into a control medicinal material solution by the same method. According to the test of thin layer chromatography (Chinese Pharmacopoeia 2020 Edition Volume IV, General Test 0502), 2 μl of the above solution was taken and spotted on the same silica gel G thin layer plate, with n-hexane-ethyl acetate (4:1) as the developing agent, developed, taken out, dried, and observed under ultraviolet light (365 nm).

[0050] Experimental results: In the chromatograms of 14 honeycomb test samples, there were fluorescent spots of the same color at the positions corresponding to the chromatogram of the control medicinal materials, but the main spot intensities of each sample were different, among which the main spot of No. 14 test sample was particularly weak, showing quality differences. This method can evaluate the quality of honeycomb to some extent. The results are shown in the attached Figure 1 .

[0051] (2) Effect comparison of different proportions of developing agent (n-hexane-ethyl acetate)

[0052] Take 4 parts of test sample of Fomes officinalis from different sources, grind them, take 0.5 g, add 30 ml of ethyl ether, soak the liquid, filter, evaporate the filtrate to dryness, add 1 ml of ethyl acetate to the residue to dissolve as the test solution. Another 0.5 g of Fomes officinalis control drug material was prepared into a control drug material solution by the same method. Test by thin layer chromatography (Chinese Pharmacopoeia 2020 Edition Volume 4, General Rule 0502), take 2 μl of the above solution respectively, spot on the same silica gel G thin layer plate, develop with different proportions of n-hexane-ethyl acetate as developing agent, take out, dry, and observe under ultraviolet light (365 nm).

[0053] Experimental results: The developing agent (n-hexane-ethyl acetate) ratio is in a wide range (19~2∶1), and the chromatography of Fomes officinalis medicinal material and Fomes officinalis control drug material is ideal. The chromatographic spots of the two can correspond well, and the developing time is 15~25 minutes. When the ratio of n-hexane-ethyl acetate is about 4∶1, the Rf value is more appropriate. The results are shown in the following table: Figures 2-5 .

[0054] (3) Comparison of the effects of different sample amounts

[0055] Take 0.5 g of Fomes officinalis control drug material, add 30 ml of ethyl ether, soak the liquid, filter, evaporate the filtrate to dryness, add 1 ml of ethyl acetate to the residue to dissolve as the test solution. Test by thin layer chromatography (Chinese Pharmacopoeia 2020 Edition Volume 4, General Rule 0502), take 0.5~10 μl of the above solution respectively, spot on the same silica gel G thin layer plate, develop with n-hexane-ethyl acetate (4∶1) as developing agent, take out, dry, and observe under ultraviolet light (365 nm).

[0056] Experimental results: The sample amount of Fomes officinalis control drug material solution is in the range of 0.5~10 μl, which has good display effect. The optimal sample amount is 1~5 μl. The results are shown in the following table: Figure 6 .

[0057] (4) Applicability of silica gel thin layer plates from different manufacturers

[0058] Take 3 parts of test sample of Fomes officinalis from different sources, grind them, take 0.5 g, add 30 ml of ethyl ether, soak the liquid, filter, evaporate the filtrate to dryness, add 1 ml of ethyl acetate to the residue to dissolve as the test solution. Another 0.5 g of Fomes officinalis control drug material was prepared into a control drug material solution by the same method. Test by thin layer chromatography (Chinese Pharmacopoeia 2020 Edition Volume 4, General Rule 0502), take 2 μl of the above solution respectively, spot on the different manufacturers' silica gel G thin layer plates, develop with n-hexane-ethyl acetate (4∶1) as developing agent, take out, dry, and observe under ultraviolet light (365 nm).

[0059] Experimental results: After developing with silica gel thin layer plates from different manufacturers, the same colored fluorescent spots appeared in the chromatogram of the test sample of Flos Farfarae at the positions corresponding to the chromatogram of the reference medicinal material, and the effects were good. The results are shown in the following table: Figures 7-9 .

[0060] (5) Comparison of effects of different developing agents

[0061] To investigate the effects of different developing agents, cyclohexane and petroleum ether (60-90°C) with similar polarity were used instead of n-hexane.

[0062] Take 3 portions of Flos Farfarae test samples from different sources, grind them, take 0.5 g, add 30 ml of ethyl ether, soak the liquid, filter, evaporate the filtrate to dryness, add 1 ml of ethyl acetate to the residue to dissolve it as the test sample solution. Take another 0.5 g of Flos Farfarae reference medicinal material, and prepare a reference medicinal material solution in the same way. According to the thin layer chromatography method (Chinese Pharmacopoeia 2020 edition volume four, general rule 0502), take 2 μl of the above solutions, respectively, and point them on the same silica gel G thin layer plate, use cyclohexane-ethyl acetate (4:1), petroleum ether (60-90°C)-ethyl acetate (4:1) as the developing agent, develop, take out, dry, and observe under ultraviolet light (365 nm).

[0063] Experimental results: After developing with the above two developing agents, the same colored fluorescent spots appeared in the chromatogram of the test sample of Flos Farfarae at the positions corresponding to the chromatogram of the reference medicinal material, and the effects were good. It is indicated that cyclohexane and petroleum ether (60-90°C) with similar polarity can be used instead of n-hexane. The results are shown in the following table: Figures 10-11 .

[0064] (6) Investigation of the applicability of the present application to the identification of Flos Farfarae in traditional Chinese medicine preparations containing Flos Farfarae medicinal materials

[0065] To investigate the applicability of the present application to the identification of Flos Farfarae in traditional Chinese medicine preparations containing Flos Farfarae medicinal materials, several marketed traditional Chinese medicine preparations containing Flos Farfarae medicinal materials such as musk rheumatism capsules, turtle shell decoction pills, and nitro yellow dampness powder were selected for thin layer chromatography identification test of Flos Farfarae contained therein.

[0066] 1) Take 0.6 g of the contents of musk rheumatism capsules, grind them, add 30 ml of ethyl ether, soak the liquid, filter, evaporate the filtrate to dryness, add 1 ml of ethyl acetate to the residue to dissolve it as the test sample solution. Take another 0.5 g of Flos Farfarae reference medicinal material, and prepare a reference medicinal material solution in the same way. According to the thin layer chromatography method (Chinese Pharmacopoeia 2020 edition volume four, general rule 0502), take 4 μl of the test sample solution and 1 μl of the reference medicinal material solution, respectively, and point them on the same silica gel G thin layer plate, use n-hexane-ethyl acetate (4:1) as the developing agent, develop, take out, dry, and observe under ultraviolet light (365 nm).

[0067] The experimental results are as follows: In the chromatogram of the test sample, the same color fluorescent spots as the chromatogram of the control drug material appear at the corresponding positions. It is proved that the method of the present application is suitable for identifying honeycomb in the Shexiang Fengshi Capsules. The results are shown in the following table: Figure 12

[0068] 2) Take 5 g of Biejia Jianwan, grind finely, add 30 ml of ethyl ether, soak the liquid, filter, evaporate the filtrate to dryness, add 1 ml of ethyl acetate to dissolve as the test sample solution. Another 0.5 g of honeycomb control drug material is prepared into a control drug material solution in the same way. According to the thin layer chromatography method (2020 edition of Chinese Pharmacopoeia, part 4, general rule 0502), 4 μl of the test sample solution and 1 μl of the control drug material solution are taken and spotted on the same silica gel G thin layer plate, with n-hexane-ethyl acetate (4:1) as the developing agent, developed, taken out, dried, and observed under ultraviolet light (365 nm).

[0069] The experimental results are as follows: In the chromatogram of the test sample, the same color fluorescent spots as the chromatogram of the control drug material appear at the corresponding positions. It is proved that the method of the present application is suitable for identifying honeycomb in the Biejia Jianwan. The results are shown in the following table: Figure 13

[0070] 3) Take 4 g of Xionghuang Qushi Powder, grind finely, add 30 ml of ethyl ether, soak the liquid, filter, evaporate the filtrate to dryness, add 1 ml of ethyl acetate to dissolve as the test sample solution. Another 0.5 g of honeycomb control drug material is prepared into a control drug material solution in the same way. Again, 4 g of negative preparation without honeycomb is prepared into a honeycomb negative control solution in the same way as the test sample solution. According to the thin layer chromatography method (2020 edition of Chinese Pharmacopoeia, part 4, general rule 0502), 2 μl of each of the above three solutions is taken and spotted on the same silica gel G thin layer plate, with n-hexane-ethyl acetate (4:1) as the developing agent, developed, taken out, dried, and observed under ultraviolet light (365 nm).

[0071] The experimental results are as follows: In the chromatogram of the test sample, the same color fluorescent spots as the chromatogram of the control drug material appear at the corresponding positions, and the negative control has no interference. The results are shown in the following table: Figure 14

[0072] The above tests show that the method of the present application not only can identify honeycomb medicinal materials, but also is suitable for identifying honeycomb in traditional Chinese medicine preparations containing honeycomb. It provides guidance and reference for identifying other traditional Chinese medicine preparations containing honeycomb.

[0073] The selected experimental examples in the specification are only a part of all experiments, and only for further description of the present application, containing the optimal scheme, and the scope of the present application is not limited by the experimental examples, and does not affect the protection of the present application.

[0074] ​​​The identification method of the present application is formed on the basis of a large number of groping, comparison and experiments, and comprises sample treatment method, control selection, development system selection and optimization, observation method comparison and the like. The method is simple, the chromatographic spot is clear, the specificity is strong, the reproducibility is good, and the universality is good. The present application overcomes the shortcomings of the prior art, solves the long-standing difficult-to-solve problem of the identification of honeycomb medicinal materials by thin layer, has an important guiding role for the identification of other traditional Chinese medicine preparations containing honeycomb medicinal materials, and achieves unexpected technical effects. For the quality control of honeycomb medicinal materials and traditional Chinese medicine preparations containing honeycomb medicinal materials, the present application is a great progress and is creative.

Claims

1. A thin-layer chromatography method for identifying honeycomb medicinal materials, characterized in that: It comprises the following steps: Step one, preparation of test solution: take the honeycomb medicinal material, grind it, take 0.2~1.0 g, add 10~50 ml of ether, soak overnight, filter, evaporate the filtrate, add 1 ml of ethyl acetate to the residue to dissolve it as the test solution; Step two, preparation of control medicinal material solution: take 0.2~1.0 g of honeycomb control medicinal material, add 10~50 ml of ether, soak overnight, filter, evaporate the filtrate, add 1 ml of ethyl acetate to the residue to dissolve it as the control medicinal material solution; Step three, identification method: take 0.5~10 μl of the test solution prepared in step one and the control medicinal material solution prepared in step two respectively, and point them on the same silica gel G thin layer plate, use n-hexane-ethyl acetate as the developing agent, the volume ratio of n-hexane and ethyl acetate in the developing agent is 19~2∶1; develop, take out, air dry, and observe under the 365 nm ultraviolet light; the test chromatogram shows the same color fluorescent spots at the positions corresponding to the control medicinal material chromatogram.

2. The thin layer chromatography method for identifying a honeycomb medicinal material according to claim 1, characterized in that: The thin layer chromatography identification method comprises the following steps: Step one, preparation of test solution: take the honeycomb medicinal material, grind it, take 0.5 g, add 30 ml of ether, soak overnight, filter, evaporate the filtrate, add 1 ml of ethyl acetate to the residue to dissolve it as the test solution; Step two, preparation of control medicinal material solution: take 0.5 g of honeycomb control medicinal material, add 30 ml of ether, soak overnight, filter, evaporate the filtrate, add 1 ml of ethyl acetate to the residue to dissolve it as the control medicinal material solution; Step three, identification method: take 2 μl of the test solution prepared in step one and the control medicinal material solution prepared in step two respectively, and point them on the same silica gel G thin layer plate, use n-hexane-ethyl acetate with a volume ratio of 4∶1 as the developing agent, develop, take out, air dry, and observe under the 365 nm ultraviolet light; the test chromatogram shows the same color fluorescent spots at the positions corresponding to the control medicinal material chromatogram.

3. The thin layer chromatography method for identifying a honeycomb medicinal material according to claim 1 or 2, characterized in that: In the developing agent, cyclohexane or 60~90 ℃ petroleum ether is used instead of n-hexane.

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