Equipment for screening strains for promoting synergism of specific medicinal materials to combine with genetic improvement

Abstract

The invention discloses a device for screening strains capable of promoting synergism of specific medicinal materials and combining genetic improvement, and belongs to the technical field of biological pharmacy, the device comprises a box body, a sealing cover hinged to one side of an opening of the box body and magnetically attracted to the box body, and a shaking table structure arranged in the box body and used for shaking a culture container; the gas inlet pipe is fixedly arranged on the side, away from the opening, of the box body and communicated with the interior of the box body, and the gas injection assembly is arranged on the outer side of the box body, connected with the shaking table structure and used for intermittently introducing oxygen or nitrogen into the box body through the gas inlet pipe. According to the genetic improvement combined equipment for screening the strains capable of promoting the effect improvement of the specific medicinal materials, through the combined design of the gas injection assembly and the gas inlet pipe, the power of a shaking table structure can be transmitted to the piston assembly, so that the piston assembly sucks gas in a gas tank and injects the gas into the gas inlet pipe, intermittent introduction of the gas is realized, and the efficiency is improved. And the stability of a culture environment and the activity of the strain are further enhanced.

Description

Technical Field

[0001] This application relates to the field of biopharmaceutical technology, specifically a device for screening strains that enhance the efficacy of specific medicinal materials in conjunction with genetic modification. Background Technology

[0002] With the continuous development of biotechnology, microbial screening and cultivation techniques have become a crucial aspect of research on synergistic strains for medicinal materials. Strain screening is typically based on their ability to enhance the efficacy of specific medicinal materials, and selecting suitable strains for cultivation is essential for improving the quality and yield of these materials. In traditional strain screening methods, a common practice is to use a shaker to mechanically vibrate the culture medium, ensuring a uniform distribution of oxygen and nutrients during cultivation.

[0003] Chinese utility model patent CN216998411U discloses a low-temperature dominant microbial strain cultivation device with screening function. Through its overall structure, the device is simple and easy to operate, reducing manual intervention and automating the strain screening process. Combined with the feeding nozzle assembly and the shaking inn main structure, it facilitates the separation and detection of microorganisms, thus effectively screening them. However, this cultivation device relies solely on the mechanical vibration of the shaking inn to achieve mixing and strain screening. In high-throughput screening, this method struggles to ensure the uniformity of dissolved oxygen and nutrients among multiple culture containers. This unevenness not only easily leads to inconsistent strain growth but also inhibits their metabolic activity, thus adversely affecting screening efficiency.

[0004] Therefore, this application provides a device for screening strains that enhance the efficacy of specific medicinal materials in conjunction with genetic improvement, in order to solve the above-mentioned problems. Summary of the Invention

[0005] This application provides a device for screening strains that enhance the efficacy of specific medicinal materials, combined with genetic improvement. It aims to solve the problems in the existing devices mentioned in the background art, which, although they achieve automated strain screening, rely solely on mechanical vibration mixing in a shaker. In high-throughput screening, it is difficult to ensure uniform dissolved oxygen and nutrients among multiple containers, resulting in uneven strain growth and suppressed metabolic activity.

[0006] To achieve the above objectives, this application provides the following technical solution: a device for screening strains that enhance the efficacy of specific medicinal materials in conjunction with genetic improvement, comprising a box, a sealing cover hinged to one side of the opening of the box and magnetically attracted to the box, and a shaking table structure disposed in the box for shaking the culture container; The device also includes an air inlet pipe fixedly disposed on the side of the box away from the opening and communicating with the inside of the box, and an air injection assembly disposed on the outside of the box and connected to the shaking table structure for intermittently introducing oxygen or nitrogen into the box through the air inlet pipe. The gas injection assembly includes a gas tank disposed on the outside of the housing, a piston assembly disposed on the outer wall of the housing and connected to the gas tank and the air inlet pipe, and a transmission assembly disposed on the housing and connected to the piston assembly and the shaking table structure for transmitting power from the shaking table structure to the piston assembly, so that the piston assembly draws gas from the gas tank and injects it into the air inlet pipe. The shaking table structure is used to shake the culture container, which helps gas exchange within the culture container, ensures uniform distribution of nutrients, and promotes the growth and metabolic activity of the strains, thereby facilitating the screening of well-growing and metabolically active strains. At the same time, through the combined design of the gas injection assembly and the air inlet pipe, the power of the shaking table structure can be transmitted to the piston assembly, so that the piston assembly draws gas from the gas tank and injects it into the air inlet pipe, realizing intermittent gas introduction, thereby further enhancing the stability of the culture environment and the activity of the strains.

[0007] Preferably, to achieve the shaking of the culture container, the shaker structure includes a support seat disposed within the housing, through holes arranged in a rectangular array on the support seat for inserting the culture container, an elastic ring fixedly connected to the inner wall of the through holes for contacting the outer wall of the culture container, and a drive assembly disposed at the bottom of the housing for shaking the support seat. The support seat provides a platform for placing the culture container, while the rectangular array of through holes facilitates neat insertion of the culture container, making management and operation easier. In addition, the elastic ring contacts the outer wall of the culture container, providing cushioning and fixation to prevent the culture container from shaking or being damaged during shaking, while ensuring close contact between the culture container and the support seat to reduce external interference. The drive assembly provides shaking power to the support seat, allowing the strains inside the culture container to fully contact the culture medium and gas, promoting the growth and metabolism of the strains.

[0008] Preferably, to achieve the rocking motion of the support, the driving assembly includes a fixed plate fixedly installed inside the container at a position corresponding to the lower part of the support; three turntables rotatably connected to the fixed plate near the support and arranged in a triangular pattern; an eccentric shaft fixedly connected to each of the three turntables and arranged eccentrically; a tripod fixedly sleeved on the three driving assemblies at the ends away from the corresponding turntables; a connecting plate fixedly installed on the tripod and fixedly connected to the bottom end of the support; a driving shaft rotatably connected inside the container at a position corresponding to the lower part of the fixed plate and fixedly connected to one of the turntables; and a motor fixedly installed inside the container for driving the driving shaft to rotate. The transmission assembly is connected to the driving shaft. By driving the driving shaft to rotate through the motor, and with the driving shaft fixedly connected to one of the turntables, and the three turntables arranged in a triangular pattern, with the eccentric shaft eccentrically positioned on the turntables, the rotation of the turntables can be converted into the rocking motion of the support, which helps with gas exchange and uniform distribution of nutrients within the culture container, thereby promoting the growth and metabolic activities of the bacterial strain.

[0009] Preferably, to achieve gas injection, the piston assembly includes a cylinder fixedly installed on the outer wall of the housing, a piston slidably connected to the cylinder, a push rod passing through one end of the cylinder and fixedly connected to the piston, and a suction pipe fixedly connected to one side of the cylinder and fixedly connected to the gas tank. The push rod is slidably connected to the cylinder and is connected to the transmission assembly. The end of the cylinder away from the push rod is fixedly connected to the end of the air inlet pipe away from the housing. Through the reciprocating motion of the push rod, the piston slides within the cylinder, enabling gas suction and injection, providing an intermittent gas supply to the housing.

[0010] Preferably, to facilitate the diffusion of gas entering the chamber through the air inlet pipe, a horn cover is fixedly connected to one end of the air inlet pipe near the chamber. The horn cover is located inside the chamber, and the diameter of the end of the horn cover near the air inlet pipe is smaller than the diameter of the opening end of the horn cover. This design of the horn cover and the diameter of the end of the horn cover near the air inlet pipe being smaller than the diameter of the opening end allows the gas entering the chamber from the air inlet pipe to diffuse as it passes through the horn cover, increasing the contact area between the gas and the culture environment inside the chamber. This allows the gas to be more evenly distributed in the culture environment of the chamber, providing a more stable gaseous environment for the bacterial strain and promoting its growth and metabolism.

[0011] Preferably, to achieve the reciprocating sliding of the push rod, the transmission assembly includes a connecting bevel gear fixedly sleeved on the drive shaft, a transmission shaft passing through the housing and rotatably connected to the housing, a transmission bevel gear fixedly sleeved on the end of the transmission shaft near the housing and meshing with the connecting bevel gear, a rotating wheel disposed on the outside of the housing and fixedly connected to the end of the transmission shaft away from the transmission bevel gear, an eccentric rod fixedly installed on the side of the rotating wheel away from the transmission shaft and eccentrically disposed, and a connecting rod rotatably connected to the eccentric rod. The end of the connecting rod away from the eccentric rod is rotatably connected to the end of the push rod away from the piston. The combined design of the connecting bevel gear, transmission shaft, transmission bevel gear, rotating wheel, eccentric rod, and connecting rod can transmit the rotation of the drive shaft to the transmission shaft through the transmission of the connecting bevel gear and the transmission bevel gear, and then convert the rotation of the transmission shaft into the reciprocating sliding of the push rod. This enables the transmission of power from the shaking table structure to the piston assembly, allowing gas to be intermittently introduced into the housing without the need for an additional power source, thus reducing the cost and energy consumption of the equipment.

[0012] Preferably, in order to further improve the diffusion effect of the gas entering the chamber, the device further includes a dispersion component disposed on the chamber and connected to the push rod for dispersing the gas at the horn cover; the design of the dispersion component can further disperse the gas at the horn cover, making the gas more evenly distributed in the culture environment, providing more suitable growth conditions for the strains, and helping to screen out strains with good growth and active metabolism.

[0013] Preferably, in order to disperse the gas at the horn cover, the dispersion component includes a rotating shaft passing through the housing on the side corresponding to the horn cover and rotatably connected to the housing; a disturbance plate fixedly installed on the rotating shaft near the end of the horn cover and located at the opening of the horn cover; and a transmission component two disposed on the outside of the housing and connected to the push rod and the rotating shaft for transmitting the power of the push rod to the rotating shaft to make the rotating shaft rotate. The power of the push rod is transmitted to the rotating shaft through the transmission component two, causing the rotating shaft to rotate. When the rotating shaft rotates, the disturbance plate can disturb the gas coming out of the horn cover, further dispersing the gas.

[0014] Preferably, in order to achieve the rotation of the shaft, the transmission assembly two includes an L-shaped frame fixedly connected to one side of the push rod and located on the outside of the housing, a toothed plate slidably connected to the outer wall of the housing and fixedly connected to the end of the L-shaped frame away from the push rod, and a connecting gear fixedly sleeved on the end of the shaft away from the disturbance plate and meshing with the toothed plate. With this design, when the push rod slides back and forth, it can drive the toothed plate to slide back and forth. Through the meshing transmission of the toothed plate and the connecting gear, the connecting gear can be driven to rotate in both directions, so that the shaft rotates. No additional power source is required, which reduces the cost and energy consumption of the equipment.

[0015] Preferably, in order to ensure the effect of airflow dispersion, multiple disturbance plates are provided, and the multiple disturbance plates are arranged in a ring on the rotating shaft; the multiple disturbance plates arranged in a ring on the rotating shaft can increase the disturbance area of ​​the gas, improve the gas dispersion effect, make the gas more evenly distributed in the box, and provide a better growth environment for the strain.

[0016] The device for screening strains that enhance the efficacy of specific medicinal materials, combined with genetic modification, uses a shaker structure to agitate the culture container, which helps with gas exchange within the container, ensures uniform distribution of nutrients, and promotes the growth and metabolic activity of the strains, thus facilitating the screening of strains with good growth and active metabolism. The device for screening strains that enhance the efficacy of specific medicinal materials, combined with genetic improvement, uses a combination design of an injection component and an air inlet pipe to transmit the power of the shaker structure to the piston component. This allows the piston component to draw gas from the gas tank and inject it into the air inlet pipe, achieving intermittent gas introduction and further enhancing the stability of the culture environment and the activity of the strains. The device for screening strains that enhance the efficacy of specific medicinal materials, combined with genetic improvement, uses a whistle-shaped cover to diffuse the gas entering the chamber from the air inlet pipe as it passes through the cover. This increases the contact area between the gas and the culture environment inside the chamber, allowing the gas to be distributed more evenly within the culture environment. This provides a more stable gaseous environment for the strains, promoting their growth and metabolism. This device for screening strains that enhance the efficacy of specific medicinal materials, combined with genetic improvement, utilizes a dispersion component design to further disperse the gas at the hood, making the gas distribution more uniform in the culture environment. This provides more suitable growth conditions for the strains and helps to screen out strains with good growth and active metabolism.

[0017] The device for screening strains that enhance the efficacy of specific medicinal materials and combining genetic improvement, through the design of transmission component one and transmission component two, can transmit the rotational power of the motor-driven shaft to the piston assembly and the disturbance plate, thus eliminating the need for an additional power source and reducing the cost and energy consumption of the equipment. Attached Figure Description

[0018] Figure 1 This is a schematic diagram of the device for screening strains that enhance the efficacy of specific medicinal materials, combined with genetic modification, as shown in Example 1. Figure 2 This is a schematic diagram of the internal structure of the box in Example 1; Figure 3 This is a cross-sectional view of the driving component in Example 1; Figure 4 This is a schematic diagram of the bearing seat in Example 1; Figure 5 This is a schematic diagram of the gas injection assembly in Example 1; Figure 6 This is a cross-sectional view of the piston assembly in Example 1; Figure 7 This is a schematic diagram of the device for screening strains that enhance the efficacy of specific medicinal materials, combined with genetic modification, as shown in Example 2. Figure 8 This is a schematic diagram of the structure of the device for the dispersion component in Example 2.

[0019] In the picture: 1. Box body; 2. Sealing cap; 3. Shaking table structure; 31. Support base; 32. Elastic ring; 33. Drive assembly; 331. Fixing plate; 332. Turntable; 333. Eccentric shaft; 334. Triangular frame; 335. Connecting plate; 336. Drive shaft; 337. Motor; 4. Air intake pipe; 41. Horn cover; 5. Injection assembly; 51. Piston assembly; 511. Cylinder; 512. Piston; 513. Push rod; 514. Suction pipe; 52. Gas tank; 53. Transmission assembly one; 531. Connecting bevel gear; 532. Transmission bevel gear; 533. Transmission shaft; 534. Rotary wheel; 535. Eccentric rod; 536. Connecting rod; 6. Dispersion component; 61. Rotating shaft; 62. Disturbance plate; 63. Transmission component two; 631. L-shaped frame; 632. Tooth plate; 633. Connecting gear. Detailed Implementation

[0020] The technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, and not all embodiments. Based on the embodiments of this application, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this application. Example

[0021] This embodiment provides a device for screening strains that enhance the efficacy of specific medicinal materials, combined with genetic improvement. Figures 1-6 As shown, the device includes a housing 1, a sealing cover 2 hinged to the opening side of the housing 1 and magnetically attracted to the housing 1, and a shaking table structure 3 disposed inside the housing 1 for shaking the culture container; the device also includes an air inlet pipe 4 fixedly disposed on the side of the housing 1 away from the opening and communicating with the interior of the housing 1, and an air injection assembly 5 disposed on the outside of the housing 1 and connected to the shaking table structure 3 for intermittently introducing oxygen or nitrogen into the housing 1 through the air inlet pipe 4; the air injection assembly 5 includes a gas tank 52 disposed on the outside of the housing 1, a piston assembly 51 disposed on the outer wall of the housing 1 and communicating with the gas tank 52 and the air inlet pipe 4, and a transmission assembly 53 disposed on the housing 1 and connected to the piston assembly 51 and the shaking table structure 3 for transmitting power from the shaking table structure 3 to the piston assembly 51, so that the piston assembly 51 draws gas from the gas tank 52 and injects it into the air inlet pipe 4.

[0022] In use, place the culture medium to be cultured into the culture container, then place the culture container on the shaker structure 3, and close the sealing cap 2. Magnetically attach the sealing cap 2 to the box body 1 to maintain the seal of the box body 1. Next, start the shaker structure 3 to shake the culture container on the shaker structure 3 to promote gas exchange and uniform distribution of nutrients within the culture container. Since the transmission component 53 is connected to the shaker structure 3, when the shaker structure 3 is started to drive the culture container to shake, the transmission component 53 will transmit the power of the shaker structure 3 to the piston assembly 51, and the piston assembly 51 will deliver gas to the air inlet pipe 4. The gas can be oxygen or nitrogen, allowing the gas to be indirectly delivered into the box body 1 from the air inlet pipe 4. As the culture container shakes, the culture medium in the culture container can mix with the introduced gas to promote the growth and metabolism of the strains, providing suitable environmental conditions and helping to screen out strains with good growth and active metabolism.

[0023] Specifically, the shaker structure 3 includes a support base 31 disposed within the housing 1, through holes arranged in a rectangular array on the support base 31 for inserting culture containers, elastic rings 32 fixedly connected to the inner wall of the through holes for contacting the outer wall of the culture containers, and a drive assembly 33 disposed at the bottom of the housing 1 for shaking the support base 31; the drive assembly 33 includes a fixed plate 331 fixedly installed within the housing 1 at a position corresponding to the lower position of the support base 31, three turntables 332 rotatably connected to the side of the fixed plate 331 near the support base 31 and arranged in a triangular pattern, and a distribution... The system includes an eccentric shaft 333 fixedly connected to the three turntables 332 and eccentrically arranged; a tripod 334 fixedly sleeved on the end of the three drive components 33 away from the corresponding turntable 332; a connecting plate 335 fixedly installed on the tripod 334 and fixedly connected to the bottom end of the support seat 31; a drive shaft 336 rotatably connected to the position below the corresponding fixed plate 331 inside the housing 1 and fixedly connected to one of the turntables 332; and a motor 337 fixedly installed inside the housing 1 for driving the drive shaft 336 to rotate. The transmission component 53 is connected to the drive shaft 336. After the culture medium is placed into the culture container, the container is then inserted into the through hole on the support 31. Because the elastic ring 32 is elastic, it will make close contact with the outer wall of the culture container, providing cushioning and stability during shaking. Then, after starting the motor 337, the motor will drive the drive shaft 336 to rotate. Since the drive shaft 336 rotates with one of the turntables 332, the rotation of the drive shaft 336 will cause the connected turntable 332 to rotate synchronously. Furthermore, due to the triangular distribution design of the three turntables 332, and the fact that each turntable 332 has… All are connected to the tripod 334 via an eccentric shaft 333, and the eccentric shaft 333 is set eccentrically. Therefore, when one of the turntables 332 rotates and drives the eccentric shaft 333 connected to it to make a circular motion, the other two turntables 332 will also drive the eccentric shaft 333 connected to them to make a circular motion in sync. Then, the three eccentric shafts 333 will drive the tripod 334 to produce irregular motion. This irregular motion is transmitted to the support seat 31 through the connecting plate 335, causing the support seat 31 to shake. As the support seat 31 shakes, the bacteria in the culture container placed in its through hole will also shake synchronously and mix thoroughly with the culture medium.

[0024] Furthermore, the piston assembly 51 includes a cylinder 511 fixedly mounted on the outer wall of the housing 1, a piston 512 slidably connected inside the cylinder 511, a push rod 513 passing through one end of the cylinder 511 and fixedly connected to the piston 512, and a suction pipe 514 fixedly connected to one side of the cylinder 511 and fixedly connected to the gas tank 52. The push rod 513 is slidably connected to the cylinder 511 and is connected to the transmission assembly 53. The end of the cylinder 511 away from the push rod 513 is fixedly connected to the end of the air inlet pipe 4 away from the housing 1. The transmission assembly 53 includes a connecting cone fixedly sleeved on the drive shaft 336. Gear 531, drive shaft 533 passing through housing 1 and rotatably connected to housing 1, drive bevel gear 532 fixedly sleeved on one end of drive shaft 533 near housing 1 and meshing with connecting bevel gear 531, rotating wheel 534 disposed on the outside of housing 1 and fixedly connected to the end of drive shaft 533 away from drive bevel gear 532, eccentric rod 535 fixedly installed on the side of rotating wheel 534 away from drive shaft 533 and eccentrically disposed, and connecting rod 536 rotatably connected to eccentric rod 535, the end of connecting rod 536 away from eccentric rod 535 being rotatably connected to the end of push rod 513 away from piston 512; When the starter motor 337 drives the drive shaft 336 to rotate, the connecting bevel gear 531, which is fixedly sleeved on the drive shaft 336, rotates accordingly. Since the connecting bevel gear 531 meshes with the transmission bevel gear 532 connected to the transmission shaft 533, the transmission shaft 533 rotates synchronously through the meshing of the transmission bevel gear 532 and the connecting bevel gear 531. Then, as the transmission shaft 533 rotates, the wheel 534 rotates, converting the rotational motion of the transmission shaft 533 into the rotational motion of the wheel 534. Because the wheel 534 is fixedly mounted on the side away from the transmission shaft 533 and is eccentrically positioned, and since the eccentric rod 535 is not at the center of the wheel 534, when the wheel 534 rotates, the eccentric rod 535 will perform a circular motion. The connecting rod 536 converts the circular motion of the eccentric rod 535 into the reciprocating linear motion of the push rod 513, which in turn drives the piston 512 to slide inside the cylinder 511. When the piston 512 moves away from the air inlet pipe 4, the space inside the cylinder 511 increases, creating a negative pressure. This allows the gas in the gas tank 52 to be drawn into the cylinder 511 through the suction pipe 514. When the piston 512 moves closer to the air inlet pipe 4, the space inside the cylinder 511 decreases, and the gas is compressed. It can then be injected into the box 1 through the air inlet pipe 4. Thus, as the push rod 513 reciprocates the piston 512, the function of intermittently injecting gas into the box 1 can be achieved, providing a suitable gas environment for the growth of strains in the culture container.

[0025] It should be noted that both the air inlet pipe 4 and the suction pipe 514 are fixedly equipped with one-way valves. During the suction stage, the one-way valve in the suction pipe 514 is open, allowing the gas in the gas tank 52 to smoothly enter the cylinder 511, while the one-way valve in the air inlet pipe 4 is closed to prevent external air or other substances from flowing back into the cylinder 511. During the gas injection stage, the one-way valve in the air inlet pipe 4 is open, allowing the gas to enter the housing 1 through the air inlet pipe 4, while the one-way valve in the suction pipe 514 is closed to prevent the gas in the cylinder 511 from flowing back into the gas tank 52, ensuring that the gas can enter the housing 1 in the predetermined direction and path, thus achieving effective gas delivery.

[0026] In order to facilitate the diffusion of gas entering the chamber 1 through the air inlet pipe 4, a horn cover 41 is fixedly connected to one end of the air inlet pipe 4 near the chamber 1. The horn cover 41 is located inside the chamber 1, and the diameter of the end of the horn cover 41 near the air inlet pipe 4 is smaller than the diameter of the opening end of the horn cover 41. The design of the horn cover 41 and the diameter of the end of the horn cover 41 near the air inlet pipe 4 being smaller than the diameter of the opening end allows the gas entering the chamber 1 from the air inlet pipe 4 to diffuse when passing through the horn cover 41, increasing the contact area between the gas and the culture environment inside the chamber 1, so that the gas can be more evenly distributed in the culture environment of the chamber 1, providing a more stable gas environment for the strains and promoting the growth and metabolism of the strains. Example

[0027] Unlike Example 1, as Figures 7-8 As shown, in order to further improve the diffusion effect of the gas entering the chamber 1, the device also includes a dispersion component 6 installed on the chamber 1 and connected to the push rod 513 for dispersing the gas at the horn cover 41; the design of the dispersion component 6 can further disperse the gas at the horn cover 41, making the gas more evenly distributed in the culture environment, providing more suitable growth conditions for the strains, and helping to screen out strains with good growth and active metabolism.

[0028] The dispersion component 6 includes a rotating shaft 61 that passes through one side of the housing 1 corresponding to the horn cover 41 and is rotatably connected to the housing 1; a disturbance plate 62 that is fixedly installed on the rotating shaft 61 near one end of the horn cover 41 and located at the opening of the horn cover 41; and a transmission component 63 that is disposed on the outside of the housing 1 and connected to the push rod 513 and the rotating shaft 61 for transmitting power from the push rod 513 to the rotating shaft 61 to make the rotating shaft 61 rotate. The transmission component 63 includes an L-shaped frame 631 that is fixedly connected to one side of the push rod 513 and located on the outside of the housing 1; a toothed plate 632 that is slidably connected to the outer wall of the housing 1 and fixedly connected to the end of the L-shaped frame 631 away from the push rod 513; and a connecting gear 633 that is fixedly sleeved on the end of the rotating shaft 61 away from the disturbance plate 62 and meshes with the toothed plate 632. When push rod 513 pushes piston 512 in reciprocating linear motion, due to the connection between L-shaped bracket 631 and push rod 513, L-shaped bracket 631 can reciprocate linearly in sync with the reciprocating linear motion of push rod 513. Since toothed plate 632 is fixedly connected to L-shaped bracket 631, toothed plate 632 will reciprocate linearly on the outer wall of housing 1 as L-shaped bracket 631 moves. Because of the meshing of connecting gear 633 connected to shaft 61, when toothed plate 632 reciprocates, connecting gear 633 will move under the action of toothed plate 632. The alternating forward and reverse rotation drives the rotating shaft 61 to rotate synchronously in both directions. As the rotating shaft 61 rotates, it further drives the disturbance plate 62, which is fixedly installed at one end, to swing at the opening of the horn cover 41. When gas enters the chamber 1 through the air inlet pipe 4 and the horn cover 41, the swinging of the disturbance plate 62 can further break the state of the gas at the horn cover 41, making the gas more evenly dispersed in the culture environment. At the same time, it promotes the full contact and mixing of the gas with the substances in the culture container, improves the culture effect, and provides a more stable and uniform environmental condition for the growth of the strain.

[0029] In addition, to ensure the effect of airflow dispersion, multiple disturbance plates 62 are provided, and the multiple disturbance plates 62 are arranged in a ring on the rotating shaft 61. The multiple disturbance plates 62 arranged in a ring on the rotating shaft 61 can increase the disturbance area of ​​the gas, improve the gas dispersion effect, make the gas more evenly distributed in the box 1, and provide a better growth environment for the strain.

[0030] The above description is merely a preferred embodiment of this application, but the scope of protection of this application is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in this application, based on the technical solution and concept of this application, should be included within the scope of protection of this application.

Claims

1. An apparatus for screening strains that enhance the efficacy of specific medicinal materials in conjunction with genetic improvement, comprising a box (1), a sealing cover (2) hinged to one side of the opening of the box (1) and magnetically attracted to the box (1), and a shaker structure (3) disposed inside the box (1) for shaking culture containers. Its features are: The device also includes an air inlet pipe (4) fixedly disposed on the side of the box (1) away from the opening and communicating with the inside of the box (1), and an air injection assembly (5) disposed on the outside of the box (1) and connected to the shaking table structure (3) for intermittently introducing oxygen or nitrogen into the box (1) through the air inlet pipe (4). The gas injection assembly (5) includes a gas tank (52) disposed on the outside of the housing (1), a piston assembly (51) disposed on the outer wall of the housing (1) and connected to the gas tank (52) and the air inlet pipe (4), and a transmission assembly (53) disposed on the housing (1) and connected to the piston assembly (51) and the shaking table structure (3) for transmitting power from the shaking table structure (3) to the piston assembly (51), so that the piston assembly (51) draws gas from the gas tank (52) and injects it into the air inlet pipe (4).

2. The device for screening strains that enhance the efficacy of specific medicinal materials combined with genetic improvement according to claim 1, characterized in that: The shaker structure (3) includes a support seat (31) disposed in the housing (1), through holes opened on the support seat (31) and arranged in a rectangular array for inserting culture containers, an elastic ring (32) fixedly connected to the inner wall of the through holes for contact with the outer wall of the culture containers, and a drive assembly (33) disposed at the bottom inside the housing (1) for shaking the support seat (31).

3. The device for screening strains that enhance the efficacy of specific medicinal materials combined with genetic improvement according to claim 2, characterized in that: The drive assembly (33) includes a fixed plate (331) fixedly installed inside the housing (1) at a position corresponding to the lower position of the support seat (31), three turntables (332) rotatably connected to the side of the fixed plate (331) near the support seat (31) and arranged in a triangular pattern, an eccentric shaft (333) fixedly connected to the three turntables (332) and arranged eccentrically, a tripod (334) fixedly sleeved on the end of the three drive assemblies (33) away from the corresponding turntable (332), a connecting plate (335) fixedly installed on the tripod (334) and fixedly connected to the bottom end of the support seat (31), a drive shaft (336) rotatably connected inside the housing (1) at a position corresponding to the lower position of the fixed plate (331) and fixedly connected to one of the turntables (332), and a motor (337) fixedly installed inside the housing (1) for driving the drive shaft (336) to rotate. The transmission assembly (53) is connected to the drive shaft (336).

4. The device for screening strains that enhance the efficacy of specific medicinal materials combined with genetic improvement according to claim 3, characterized in that: The piston assembly (51) includes a cylinder (511) fixedly installed on the outer wall of the housing (1), a piston (512) slidably connected inside the cylinder (511), a push rod (513) passing through one end of the cylinder (511) and fixedly connected to the piston (512), and a suction pipe (514) fixedly connected to one side of the cylinder (511) and fixedly connected to the gas tank (52). The push rod (513) is slidably connected to the cylinder (511), and the push rod (513) is connected to the transmission assembly (53). The end of the cylinder (511) away from the push rod (513) is fixedly connected to the end of the air inlet pipe (4) away from the housing (1).

5. The device for screening strains that enhance the efficacy of specific medicinal materials combined with genetic improvement according to claim 4, characterized in that: The intake pipe (4) is fixedly connected to a horn cover (41) at one end near the housing (1). The horn cover (41) is located inside the housing (1). The diameter of the horn cover (41) at the end near the intake pipe (4) is smaller than the diameter of the opening end of the horn cover (41).

6. The device for screening strains that enhance the efficacy of specific medicinal materials combined with genetic improvement according to claim 4, characterized in that: The transmission assembly (53) includes a connecting bevel gear (531) fixedly sleeved on the drive shaft (336), a transmission shaft (533) passing through the housing (1) and rotatably connected to the housing (1), a transmission bevel gear (532) fixedly sleeved on the transmission shaft (533) near the housing (1) and meshing with the connecting bevel gear (531), a rotating wheel (534) disposed on the outside of the housing (1) and fixedly connected to the end of the transmission shaft (533) away from the transmission bevel gear (532), an eccentric rod (535) fixedly mounted on the side of the rotating wheel (534) away from the transmission shaft (533) and eccentrically arranged, and a connecting rod (536) rotatably connected to the eccentric rod (535). The end of the connecting rod (536) away from the eccentric rod (535) is rotatably connected to the end of the push rod (513) away from the piston (512).

7. The device for screening strains that enhance the efficacy of specific medicinal materials combined with genetic improvement according to claim 5, characterized in that: The device also includes a dispersion component (6) disposed on the housing (1) and connected to the push rod (513) for dispersing gas at the horn cover (41).

8. The device for screening strains that enhance the efficacy of specific medicinal materials combined with genetic improvement according to claim 7, characterized in that: The dispersion component (6) includes a rotating shaft (61) that passes through the box (1) on one side corresponding to the horn cover (41) and is rotatably connected to the box (1); a disturbance plate (62) that is fixedly installed on the rotating shaft (61) near one end of the horn cover (41) and located at the opening of the horn cover (41); and a transmission component (63) that is disposed on the outside of the box (1) and connected to the push rod (513) and the rotating shaft (61) for transmitting the power of the push rod (513) to the rotating shaft (61) to make the rotating shaft (61) rotate.

9. The device for screening strains that enhance the efficacy of specific medicinal materials combined with genetic improvement according to claim 8, characterized in that: The transmission assembly two (63) includes an L-shaped frame (631) fixedly connected to one side of the push rod (513) and located outside the housing (1), a toothed plate (632) slidably connected to the outer wall of the housing (1) and fixedly connected to the end of the L-shaped frame (631) away from the push rod (513), and a connecting gear (633) fixedly sleeved on the end of the rotating shaft (61) away from the disturbance plate (62) and meshing with the toothed plate (632).

10. The device for screening strains that enhance the efficacy of specific medicinal materials combined with genetic improvement according to claim 9, characterized in that: Multiple disturbance plates (62) are provided, and the multiple disturbance plates (62) are arranged in a ring on the rotating shaft (61).

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