A non-invasive cell delivery system

Abstract

The application discloses a cell non-damage delivery system, which comprises an initial container, a processing device, a collecting container, a transfer container and a suction device for generating positive and negative pressure in the transfer container. The transfer container is communicated with the initial container through a first connecting pipe, communicated with the processing device through a second connecting pipe and communicated with the collecting container through a third connecting pipe. A first valve body is arranged on the first connecting pipe, a second valve body is arranged on the second connecting pipe and a third valve body is arranged on the third connecting pipe. Cells in the initial container are delivered to the processing device through the transfer container, treated in the processing device and then delivered to the collecting container through the transfer container. The cell non-damage delivery system has the advantages of avoiding cell damage caused by the suction device and greatly improving cell yield and activity.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of cell medicine preparation, and in particular to a cell non-damage delivery system. BACKGROUND

[0002] PBMC is peripheral blood mononuclear cell, and human peripheral blood contains lymphocytes, monocytes, dendritic cells and other small amount of cells. A large number of immune cells can be obtained for cell therapy by separating, screening and culturing PBMC through a blood cell separation pipeline system. The existing blood cell separation and cell culture pipeline systems directly pump cell liquid through a peristaltic pump. For example, a pipeline system for blood separation in application No. CN202121194589.5 directly delivers cell liquid through a peristaltic pump. The peristaltic pump delivers cell liquid by extruding a flexible tube, which can easily cause cell damage and reduce cell yield and viability. SUMMARY

[0003] The technical problem to be solved by the present application is to overcome the shortcomings of the prior art and provide a cell non-damage delivery system which avoids cell damage caused by a suction device and greatly improves cell yield and viability.

[0004] To solve the above technical problems, the present application adopts the following technical scheme:

[0005] A cell non-damage delivery system, comprising an initial container, a processing device, a collection container, a transfer container and a suction device for generating positive and negative pressure in the transfer container, the transfer container being in communication with the initial container through a first connecting pipe, in communication with the processing device through a second connecting pipe and in communication with the collection container through a third connecting pipe, a first valve body being arranged on the first connecting pipe, a second valve body being arranged on the second connecting pipe and a third valve body being arranged on the third connecting pipe, cells in the initial container being delivered to the processing device through the transfer container for processing and then delivered from the processing device to the collection container through the transfer container.

[0006] As a further improvement of the above technical scheme:

[0007] The processing device is a cell culture container or a cell centrifugation container.

[0008] The transfer container is provided with a circulation pipeline, and the first connecting pipe, the second connecting pipe and the third connecting pipe are respectively in communication with the transfer container through the circulation pipeline.

[0009] The suction device comprises a pump body and a clean gas pipe, and the pump body is in communication with the transfer container through the clean gas pipe.

[0010] The cell non-damage delivery system further comprises a waste liquid container, the waste liquid container is communicated with the treatment device through a waste liquid pipe, a waste liquid valve is arranged on the waste liquid pipe, and the pump body is arranged on the waste liquid pipe.

[0011] The first connecting pipe is connected with a first branch pipe for connecting with a sample source, and a fourth valve body is arranged on the first branch pipe.

[0012] The pump body is connected with a first container through a first branch pipe and communicated with the outside through a second branch pipe, a fifth valve body is arranged on the first branch pipe, and a sixth valve body is arranged on the second branch pipe.

[0013] The cell non-damage delivery system further comprises a waste liquid container, the waste liquid container is communicated with the treatment device through a waste liquid pipe, a waste liquid valve is arranged on the waste liquid pipe, and the pump body is arranged on the waste liquid pipe.

[0014] The first connecting pipe is connected with a first branch pipe for connecting with a sample source, and a fourth valve body is arranged on the first branch pipe.

[0015] The cell non-damage delivery system further comprises a waste liquid container, the waste liquid container is communicated with the treatment device through a waste liquid pipe, a waste liquid valve is arranged on the waste liquid pipe, and the pump body is arranged on the waste liquid pipe.

[0016] Compared with the prior art, the cell non-damage delivery system has the advantages that:

[0017] The production process of the cell non-damage delivery system is as follows: first, the first valve body, the second valve body and the third valve body are closed, and the negative pressure is generated in the transfer container through the suction device; second, the first valve body is opened, and the cells in the initial container flow into the transfer container under the action of the negative pressure of the transfer container, and then the first valve body is closed; then, the second valve body is opened, the positive pressure is generated in the transfer container through the suction device, and the cells in the transfer container enter the treatment device, and then the second valve body is closed; after the treatment device is treated, the negative pressure is generated in the transfer container through the suction device again; then, the second valve body is opened, and the cells in the treatment device flow into the transfer container under the action of the negative pressure of the transfer container, and then the second valve body is closed; finally, the third valve body is opened, and the positive pressure is generated in the transfer container through the suction device, so that the cells in the transfer container enter the collection container. The cell non-damage delivery system, the cells are delivered through the positive and negative pressure actions of the transfer container, do not need to flow through the suction device, avoid the damage of the cells caused by the suction device, and greatly improve the cell yield and the survival rate. BRIEF DESCRIPTION OF DRAWINGS

[0018] Figure 1 is a structural schematic view of the first embodiment of the cell non-damage delivery system.

[0019] Figure 2is a structural schematic diagram of embodiment two of the cell non-invasive delivery system of the present application.

[0020] Figure 3 is a structural schematic diagram of embodiment three of the cell non-invasive delivery system of the present application.

[0021] Figure 4 is a structural schematic diagram of embodiment four of the cell non-invasive delivery system of the present application.

[0022] The various reference numbers in the drawings represent:

[0023] 1, initial container; 11, first connecting pipe; 111, first valve body; 12, first sub-pipe; 121, fourth valve body; 13, second sub-pipe; 131, third container; 132, eighth valve body; 14, third sub-pipe; 141, fourth container; 142, ninth valve body; 2, processing device; 21, second connecting pipe; 211, second valve body; 3, collection container; 31, third connecting pipe; 311, third valve body; 4, transfer container; 41, circulating pipeline; 411, tenth valve body; 412, eleventh valve body; 5, suction device; 51, pump body; 52, clean gas pipe; 6, waste liquid container; 61, waste liquid pipe; 611, waste liquid valve; 7, first sub-pipe; 71, first container; 72, fifth valve body; 8, second sub-pipe; 81, sixth valve body. DETAILED DESCRIPTION

[0024] The present application will be further described in detail below in conjunction with the drawings and specific embodiments.

[0025] As shown in the present disclosure and claims, unless the context clearly indicates otherwise, the words "one", "an", "a", and / or "the" do not mean only singular, but can also include plural. The words "first", "second", and similar words used in the present disclosure do not mean any order, number, or importance, but are only used to distinguish different components. Similarly, the words "include" or "contain" and similar words mean that the components or objects appearing before the words cover the components or objects listed after the words and their equivalents, and do not exclude other components or objects. The words "connect" or "connected" and similar words are not limited to physical or mechanical connections, but can include electrical connections, whether direct or indirect.

[0026] Embodiment one:

[0027] Figure 1An embodiment of the cell non-destructive delivery system of the present invention is shown. The cell non-destructive delivery system of this embodiment includes an initial container 1, a processing device 2, a collection container 3, a transfer container 4, and a suction device 5 for generating positive and negative pressure in the transfer container 4. The transfer container 4 is connected to the initial container 1 through a first connecting pipe 11, to the processing device 2 through a second connecting pipe 21, and to the collection container 3 through a third connecting pipe 31. A first valve body 111 is provided on the first connecting pipe 11, a second valve body 211 is provided on the second connecting pipe 21, and a third valve body 311 is provided on the third connecting pipe 31. Cells in the initial container 1 are transported to the processing device 2 through the transfer container 4 for processing, and then transported from the processing device 2 to the collection container 3 through the transfer container 4.

[0028] Production process: First, open the first valve body 111 and close the second valve body 211 and the third valve body 311. A negative pressure is generated inside the transfer container 4 by the suction device 5, causing the cells in the initial container 1 to flow into the transfer container 4 under this negative pressure. Then, close the first valve body 111 and open the second valve body 211. A positive pressure is generated inside the transfer container 4 by the suction device 5, allowing the cells in the transfer container 4 to enter the processing device 2. After processing by the processing device 2, open the second valve body 211 and close the first valve body 111 and the third valve body 311. A negative pressure is generated inside the transfer container 4 again by the suction device 5, allowing the cells in the processing device 2 to flow into the transfer container 4 under this negative pressure. Finally, close the second valve body 211 and open the third valve body 311. A positive pressure is generated inside the transfer container 4 by the suction device 5, allowing the cells in the transfer container 4 to enter the collection container 3. This cell transport system uses the positive and negative pressure of the transfer container 4 to transport cells without them needing to pass through the suction device 5, thus avoiding cell damage caused by the suction device 5 and significantly improving cell yield and viability.

[0029] Example 2:

[0030] Figure 2 Another embodiment of the cell non-destructive delivery system of the present invention is shown. This embodiment is basically the same as the first embodiment, except that the transfer container 4 is provided with a circulation pipe 41, and the first connecting pipe 11, the second connecting pipe 21, and the third connecting pipe 31 are respectively connected to the transfer container 4 through the circulation pipe 41. Figure 2As shown, one end of the circulation pipe 41 is connected to the inlet above the transfer container 4, and the other end is connected to the outlet below the transfer container 4. That is, the circulation pipe 41 connects the inlet and outlet of the transfer container 4. Since the processing device 2 needs to handle both inlet (cells flow from the initial container 1 into the processing device 2) and outlet (cells flow from the processing device 2 into the collection container 3), during inlet, the liquid enters the transfer container 4 from the initial container 1 through the circulation pipe 41 and the inlet of the transfer container 4, and then enters the processing device 2 through the outlet of the transfer container 4 and the circulation pipe 41. During outlet, the liquid enters the transfer container 4 from the processing device 2 through the circulation pipe 41 and the inlet of the transfer container 4, and then enters the collection container 3 through the outlet of the transfer container 4 and the third connecting pipe 31. Therefore, whether it is inlet or outlet, the liquid enters through the inlet of the transfer container 4 and exits through the outlet, avoiding the liquid from entering through the outlet of the transfer container 4. If the liquid from the processing device 2 enters through the outlet of the transfer container 4, it will eventually draw in a large number of air bubbles, and the bursting of these bubbles will have an adverse effect on the cells.

[0031] In this embodiment, the suction device 5 includes a pump body 51 and a clean air pipe 52. The pump body 51 is connected to the transfer container 4 through the clean air pipe 52. The pump body 51 generates positive and negative pressure inside the transfer container 4 through the clean air pipe 52. The structure is simple and easy to assemble and disassemble.

[0032] In this embodiment, the cell non-destructive delivery system also includes a waste liquid container 6, which is connected to the processing device 2 via a waste liquid pipe 61. A waste liquid valve 611 is provided on the waste liquid pipe 61, and a pump body 51 is installed on the waste liquid pipe 61.

[0033] In this embodiment, the first connecting pipe 11 is connected to a first branch pipe 12 for connecting to the sample source, and the first branch pipe 12 is provided with a fourth valve body 121.

[0034] In this embodiment, the pump body 51 is connected to the first container 71 through the first branch pipe 7 and communicates with the outside through the second branch pipe 8. The first branch pipe 7 is provided with a fifth valve body 72 and the second branch pipe 8 is provided with a sixth valve body 81.

[0035] In this embodiment, the processing device 2 is a cell centrifuge container.

[0036] The circulation pipeline 41 is equipped with a tenth valve body 411 and an eleventh valve body 412. The circulation pipeline 41 between the tenth valve body 411 and the eleventh valve body 412 is connected to both the second connecting pipe 21 and the third connecting pipe 31. One end of the circulation pipeline 41 is connected to the outlet of the transfer container 4, and the other end is connected to the inlet of the transfer container 4. The circulation pipeline 41 between the eleventh valve body 412 and the inlet of the transfer container 4 is connected to the first connecting pipe 11.

[0037] The specific implementation process is as follows:

[0038] 1. Open the corresponding valves and add the reagents in the initial container 1 to the processing device 2 through the transfer container 4 via the pump body 51, and then close all valves;

[0039] 2. Open the corresponding valves and add the sample from the blood bag into the processing device 2 through the first branch pipe 12 and the transfer container 4 via the pump body 51; then close all valves.

[0040] 3. Start processing device 2 to mix the sample and reagent in processing device 2.

[0041] 4. High-speed centrifugation causes the processed sample in the processing device 2 to precipitate, and the centrifugation speed is reduced. The corresponding valves are opened, and the supernatant is discharged into the waste liquid container 6 through the pump body 51. All valves are closed, and centrifugation is stopped.

[0042] 5. Open the corresponding valves and add the reagents in the initial container 1 to the processing device 2 through the transfer container 4 via the pump body 51, and then close all valves;

[0043] 6. Open the corresponding valve and pump the liquid in the treatment device 2 into the transfer container 4 through the pump body 51, and then pump it back into the treatment device 2. Repeat the blowing and blowing to resuspend the settled cells in the treatment device 2 and let it stand for a period of time.

[0044] 7. High-speed centrifugation causes the processed sample in the processing device 2 to precipitate, and the centrifugation speed is reduced. The corresponding valves are opened, and the supernatant is discharged into the waste liquid container 6 through the pump body 51. All valves are closed, and centrifugation is stopped.

[0045] 8. Repeat steps 5 through 7 multiple times;

[0046] 9. Open the corresponding valves and add the reagent in the first container 71 into the processing device 2 through the pump body 51, then close all valves;

[0047] 10. Open the corresponding valves, and through the pump body 51, draw the liquid in the treatment device 2 into the transfer container 4, and pump it back into the treatment device 2. After repeatedly blowing and agitating several times, discharge all the liquid in the transfer container 4 into the treatment device 2 through the second connecting pipe 21, and close all valves.

[0048] 11. Start the high-speed centrifuge to centrifuge the liquid in the treatment device 2 at high speed, then reduce the centrifugation speed, open the corresponding valves, and discharge the supernatant into the waste liquid container 6 through the pump body 51. Close all valves and stop centrifugation.

[0049] 12. Repeat steps 9 to 11 several times;

[0050] 13. Open the corresponding valves and add the reagent in the first container 71 into the processing device 2 through the pump body 51, then close all valves;

[0051] 14. Open the corresponding valves, and through the pump body 51, draw the liquid in the treatment device 2 into the transfer container 4, and pump it back into the treatment device 2. After repeatedly blowing and agitating several times, discharge all the liquid in the transfer container 4 into the treatment device 2 through the second connecting pipe 21, and close all valves.

[0052] 15. Open the corresponding valves and use the pump body 51 to draw all the liquid in the treatment device 2 into the transfer container 4, then close all valves;

[0053] 16. Open the corresponding valves and discharge all the liquid in the transfer container 4 into the collection container 3 through the pump body 51, then close all valves;

[0054] Steps 13 through 16 above can be repeated several times.

[0055] Example 3:

[0056] Figure 3 Another embodiment of the cell non-destructive delivery system of the present invention is shown. This embodiment is basically the same as the first embodiment, except that: the transfer container 4 is provided with a circulation pipe 41, and the first connecting pipe 11, the second connecting pipe 21, and the third connecting pipe 31 are respectively connected to the transfer container 4 through the circulation pipe 41. The cell non-destructive delivery system also includes a waste liquid container 6, which is connected to the transfer container 4 through a waste liquid pipe 61, and a waste liquid valve 611 is provided on the waste liquid pipe 61. Figure 3 As shown, one end of the circulation pipe 41 is connected to the inlet above the transfer container 4, and the other end is connected to the outlet below the transfer container 4. That is, the circulation pipe 41 connects the inlet and outlet of the transfer container 4. Since the processing device 2 needs to handle both inlet (cells flow from the initial container 1 into the processing device 2) and outlet (cells flow from the processing device 2 into the collection container 3), during inlet, the liquid enters the transfer container 4 from the initial container 1 through the circulation pipe 41 and the inlet of the transfer container 4, and then enters the processing device 2 through the outlet of the transfer container 4 and the circulation pipe 41. During outlet, the liquid enters the transfer container 4 from the processing device 2 through the circulation pipe 41 and the inlet of the transfer container 4, and then enters the collection container 3 through the outlet of the transfer container 4 and the third connecting pipe 31. Therefore, whether it is inlet or outlet, the liquid enters through the inlet of the transfer container 4 and exits through the outlet, avoiding the liquid from entering through the outlet of the transfer container 4. If the liquid from the processing device 2 enters through the outlet of the transfer container 4, it will eventually draw in a large number of air bubbles, and the bursting of these bubbles will have an adverse effect on the cells.

[0057] In this embodiment, the suction device 5 includes a pump body 51 and a clean air pipe 52. The pump body 51 is connected to the transfer container 4 through the clean air pipe 52.

[0058] The circulation pipeline 41 is equipped with a tenth valve body 411 and an eleventh valve body 412. The circulation pipeline 41 between the tenth valve body 411 and the eleventh valve body 412 is connected to the second connecting pipe 21, the third connecting pipe 31 and the first connecting pipe 11. One end of the circulation pipeline 41 is connected to the outlet of the transfer container 4 and the other end is connected to the inlet of the transfer container 4.

[0059] In this embodiment, the first connecting tube 11 is connected to the sample source through the first branch tube 12, the third container 131 is connected through the second branch tube 13, and the fourth container 141 is connected through the third branch tube 14. The first branch tube 12 is provided with a fourth valve body 121, the second branch tube 13 is provided with an eighth valve body 132, and the third branch tube 14 is provided with a ninth valve body 142.

[0060] In this embodiment, the processing device 2 is a cell centrifuge container.

[0061] Specific Implementation Plan 1:

[0062] 1. Open the corresponding valve. Under the negative pressure of the pump body 51, first load the blood sample into the transfer container 4 through the first branch pipe 12. Then, under the positive pressure of the pump body 51, load the sample into the cell centrifuge container through the second connecting pipe 21. After completion, close the valve.

[0063] 2. Open the corresponding valve. Under the negative pressure of the pump body 51, first add the separation liquid in the initial container 1 to the transfer container 4 through the first connecting pipe 11. Then, under the positive pressure of the pump body 51, slowly load the separation liquid in the transfer container 4 into the cell centrifuge container through the second connecting pipe 21, so that the loaded separation liquid is located at the bottom of the cell centrifuge container and the blood sample to be processed is located on the upper layer of the separation liquid. After completion, close the valve.

[0064] 3. Start the cell centrifuge container for high-speed centrifugation. Under the action of centrifugal force, in the radial direction of rotation of the cell centrifuge container, from the cell to the tip of the cell centrifuge container, the layers are supernatant, PBMC, separation liquid, and red blood cell layer in sequence.

[0065] 4. Open the corresponding valve. Under the negative pressure of the pump body 51, first draw the red blood cells and part of the separation liquid from the bottom of the cell centrifuge container into the transfer container 4 through the second connecting pipe 21. Then, under the positive pressure of the pump body 51, discharge the liquid in the transfer container 4 into the waste liquid container 6. After completion, close the valve.

[0066] 5. Stop the rotation of the cell centrifuge container. First, add the buffer solution in the third container 131 to the transfer container 4 through the second branch tube 13. Then, under the positive pressure of the pump body 51, load the buffer solution in the transfer container 4 into the cell centrifuge container through the second connecting tube 21 to mix the cell solution and buffer solution in the cell centrifuge container. After completion, close the valve.

[0067] 6. Open the corresponding valve. Under the positive pressure of the pump body 51, slowly add the reagent to the tip of the cell centrifuge container through the second connecting tube 21. After loading, from the bottom of the cell centrifuge container to the tip of the cell centrifuge container, the layers are cell mixture layer and reagent layer, respectively. After completion, close the valve.

[0068] 7. Start the cell centrifuge container for high-speed centrifugation. Under the action of centrifugal force, in the radial direction of rotation of the cell centrifuge container, from the bottom of the cell centrifuge container to the tip of the cell centrifuge container, the layers are, in sequence, the supernatant layer, the PBMC layer, and the reagent layer.

[0069] 8. Open the corresponding valve. Under the negative pressure of the pump body 51, first draw the supernatant in the cell centrifuge container into the transfer container 4. Then, under the positive pressure of the pump body 51, discharge the liquid in the transfer container 4 into the waste container 6. A small amount of supernatant, PBMC and reagents remain in the cell centrifuge container. After completion, close the valve.

[0070] 9. Open the corresponding valve. Under the negative pressure of the pump body 51, the liquid in the cell centrifuge container is drawn from the bottom of the cell centrifuge container into the transfer container 4 through the second connecting pipe 21. Then, under the positive pressure of the pump body 51, the liquid in the transfer container 4 is discharged into the collection container 3. After completion, close the valve.

[0071] Specific Implementation Plan Two:

[0072] 1. Open the corresponding valve. Under the negative pressure of the pump body 51, first load the blood sample into the transfer container 4 through the first branch pipe 12. Then, under the positive pressure of the pump body 51, load the sample into the cell centrifuge container through the second connecting pipe 21. After completion, close the valve.

[0073] 2. Open the corresponding valve. Under the positive pressure of the pump body 51, slowly add the reagent in the transfer container 4 to the tip of the cell centrifuge container through the second connecting tube 21. After loading, from the bottom of the cell centrifuge container to the tip of the cell centrifuge container, the layers are cell liquid layer and reagent layer in sequence. After completion, close the valve.

[0074] 3. Start the cell centrifuge container for high-speed centrifugation. Under the action of centrifugal force, in the radial direction of rotation of the cell centrifuge container, from the bottom of the cell centrifuge container to the tip of the cell centrifuge container, the layers are, in sequence, the supernatant layer, the target cell layer, and the reagent layer.

[0075] 4. Open the corresponding valve. Under the negative pressure of the pump body 51, first draw the supernatant in the cell centrifuge container into the transfer container 4 through the second connecting tube 21. Then, under the positive pressure of the pump body 51, discharge the liquid in the transfer container 4 into the waste liquid container 6. A small amount of supernatant, target cells and reagents remain in the cell centrifuge container. After completion, close the valve.

[0076] 5. Open the corresponding valve. Under the negative pressure of the pump body 51, the liquid in the cell centrifuge container is drawn from the bottom of the cell centrifuge container into the transfer container 4 through the second connecting pipe 21. Then, under the positive pressure of the pump body 51, the liquid in the transfer container 4 is discharged into the collection container 3. After completion, close the valve.

[0077] Example 4:

[0078] Figure 4 Another embodiment of the cell non-destructive delivery system of the present invention is shown. This embodiment is essentially the same as the first embodiment, except that: the cell non-destructive delivery system includes a waste liquid container 6, which is connected to the first connecting pipe 11 via a waste liquid pipe 61. The processing device 2 is a cell culture container. The suction device 5 includes a pump body 51 and a clean air pipe 52, which is connected to the transfer container 4 via the clean air pipe 52. The pump body 51 generates positive and negative pressure within the transfer container 4 via the clean air pipe 52, resulting in a simple structure and convenient assembly and disassembly. The waste liquid container 6 is connected to the first connecting pipe 11 via the waste liquid pipe 61, allowing it to share a single first valve body 111, reducing the use of valves and lowering costs.

[0079] While the present invention has been disclosed above with reference to preferred embodiments, it is not intended to limit the invention. Any person skilled in the art can make many possible variations and modifications to the technical solutions of the present invention, or modify them into equivalent embodiments, without departing from the scope of the present invention. Therefore, any simple modifications, equivalent changes, and modifications made to the above embodiments based on the technical essence of the present invention, without departing from the scope of the present invention, should fall within the protection scope of the present invention.

Claims

1. A cell non-destructive delivery system, characterized in that: The system includes an initial container (1), a processing device (2), a collection container (3), a transfer container (4), and a suction device (5) for generating positive and negative pressure within the transfer container (4). The transfer container (4) is connected to the initial container (1) via a first connecting pipe (11), to the processing device (2) via a second connecting pipe (21), and to the collection container (3) via a third connecting pipe (31). A first valve body (111) is provided on the first connecting pipe (11), a second valve body (211) is provided on the second connecting pipe (21), and a third valve body (311) is provided on the third connecting pipe (31). Cells in container (1) are transported to processing device (2) via transfer container (4) for processing, and then transported from processing device (2) to collection container (3) via transfer container (4); the suction device (5) includes pump body (51) and clean air pipe (52), the pump body (51) is connected to transfer container (4) via clean air pipe (52); the cell non-destructive transport system also includes waste liquid container (6), the waste liquid container (6) is connected to processing device (2) via waste liquid pipe (61), the waste liquid pipe (61) is provided with waste liquid valve (611), and the pump body (51) is installed on waste liquid pipe (61).

2. The cell non-destructive delivery system according to claim 1, characterized in that: The processing device (2) is a cell culture container or a cell centrifuge container.

3. The cell non-destructive delivery system according to claim 1, characterized in that: The transfer container (4) is provided with a circulation pipeline (41), and the first connecting pipe (11), the second connecting pipe (21) and the third connecting pipe (31) are respectively connected to the transfer container (4) through the circulation pipeline (41).

4. The cell non-destructive delivery system according to claim 1, characterized in that: The first connecting tube (11) is connected to a first branch tube (12) for connecting to a sample source, and a fourth valve body (121) is provided on the first branch tube (12).

5. The cell non-destructive delivery system according to claim 4, characterized in that: The pump body (51) is connected to the first container (71) through the first branch pipe (7) and communicates with the outside through the second branch pipe (8). The first branch pipe (7) is provided with a fifth valve body (72) and the second branch pipe (8) is provided with a sixth valve body (81).

6. The cell non-destructive delivery system according to claim 1, characterized in that: The cell non-destructive delivery system also includes a waste liquid container (6), which is connected to the transfer container (4) through a waste liquid pipe (61), and a waste liquid valve (611) is provided on the waste liquid pipe (61).

7. The cell non-destructive delivery system according to claim 6, characterized in that: The first connecting tube (11) is connected to the sample source through the first branch tube (12), the third container (131) is connected through the second branch tube (13), and the fourth container (141) is connected through the third branch tube (14). The first branch tube (12) is provided with a fourth valve body (121), the second branch tube (13) is provided with an eighth valve body (132), and the third branch tube (14) is provided with a ninth valve body (142).

8. The cell non-destructive delivery system according to claim 1, characterized in that: The cell non-destructive delivery system includes a waste liquid container (6), which is connected to a first connecting pipe (11) via a waste liquid pipe (61).

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