Compositions with skin care effect, liposomes and methods for their preparation, use

Abstract

The application relates to a composition with skin care effect, a liposome and a preparation method and application thereof. The composition with skin care effect comprises golden microalgae, yeast / rice fermentation products and guaiazulene. The three components can play a synergistic effect, can have multiple skin care effects such as anti-inflammatory, antioxidant, repair, redness removal and soothing on the skin, and the product is safe and non-irritating.

Description

Technical Field

[0001] This application relates to the field of skincare technology, and in particular to a composition with skincare effects, liposomes, their preparation methods, and applications. Background Technology

[0002] Skincare products are an indispensable part of people's daily lives. With constantly changing consumer demands, the efficacy of skincare products has become a primary basis for consumers' purchasing decisions. Generally, consumers hope that the skincare products they choose will have better performance in areas such as anti-oxidation, anti-inflammation, redness reduction, repair, whitening, and moisturizing. However, traditional skincare products either have single and weak efficacy or significant side effects and low safety. For example, skincare products containing aloe vera extract and trehalose dressings can only provide moisturizing and short-term soothing and repair effects, with very limited efficacy. They only work on the surface of damaged skin and cannot achieve deep, fundamental redness reduction and repair. On the other hand, ingredients with strong efficacy often have a certain degree of irritation and are not suitable for repairing post-operative wounds. Furthermore, they can lead to dependency. For example, some skincare products containing hormones can cause skin cells to become dependent on specific ingredients. Although they may have good repair effects, they can damage the skin's ecosystem, thereby reducing its own repair capabilities. Summary of the Invention

[0003] Based on this, this application provides a skin care composition, skin care liposome, preparation method and application that have multiple skin care effects and high safety.

[0004] One embodiment of this application provides a composition with skin care effects, comprising: golden microalgae, yeast / rice fermentation product and guaiac.

[0005] In one embodiment, the mass ratio of the golden microalgae, the yeast / rice fermentation product, and the guaiac is (0.1~10):(0.1~10):(0.01~0.25).

[0006] One embodiment of this application provides a liposome with skin care effects, comprising a lecithin membrane and a skin care effect composition as described in any of the above embodiments, wherein the lecithin membrane encapsulates the skin care effect composition.

[0007] In one embodiment, the composition having skin-care effects accounts for 60% to 70% of the sum of the mass of the lecithin membrane and the composition having skin-care effects.

[0008] In one embodiment, the liposomes have a particle size of 0.1 micrometers to 1 micrometer.

[0009] One embodiment of this application provides a method for preparing liposomes with skin-care effects as described in any of the above embodiments, comprising the following steps:

[0010] Provide the lecithin membrane;

[0011] The skin-care-enhancing composition is mixed with a phosphate buffer solution to form a mixed solution;

[0012] The mixed solution was added to a container containing the lecithin membrane and then subjected to hydration and ultrasonic treatment.

[0013] In one embodiment, the pH of the phosphate buffer solution is 5.5 to 8.5.

[0014] In one embodiment, the hydration treatment conditions include a temperature of 30°C to 80°C and a time of 25 min to 35 min.

[0015] In one embodiment, the conditions for ultrasonic treatment include: a power of 600W to 800W and a time of 3.5min to 4.5min.

[0016] An embodiment of this application also provides the application of the skin-care effect composition or the skin-care effect liposome described in any of the above embodiments in the preparation of skin-care products.

[0017] An embodiment of this application also provides a skin care product, wherein the raw materials of the skin care product include the composition with skin care effect described in any of the above embodiments or the liposome with skin care effect described in any of the above embodiments.

[0018] The skin-care composition provided in this application comprises golden microalgae, yeast / rice fermentation products, and guaiac. The combination of these three components can exert a synergistic effect, providing multiple skin-care benefits such as anti-inflammatory, antioxidant, repair, redness reduction, and soothing effects. Moreover, the product is safe and non-irritating. Detailed Implementation

[0019] To facilitate understanding of this application, a more comprehensive description of the application will be provided below with reference to embodiments. However, this application can be implemented in many different forms and is not limited to the embodiments described herein. Rather, these embodiments are provided to provide a more thorough and complete understanding of the disclosure of this application.

[0020] Unless otherwise defined, all technical and scientific terms used in this application have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the application. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0021] In this application, the technical features described in an open-ended manner include both closed technical solutions consisting of the listed features and open technical solutions that include the listed features.

[0022] One embodiment of this application provides a composition with skin care effects, comprising: golden microalgae, yeast / rice fermentation product and guaiac.

[0023] Golden microalgae (Tetraselmis suecica) is an algae species rich in active ingredients, including polysaccharides, oligosaccharides, chlorophyll, amino acids, proteins, minerals, and vitamins. Notably, it also contains 2-10 mg / L of gold. The production method of golden microalgae used in this invention employs specific light and pH conditions in a closed bioreactor, where algal extracts and gold are co-cultured. This process transforms the "mineral gold" into low-irritant, high-nutrient, and highly active bio-gold. The algae used for the extract are preferably from the following phylum: Chlorophyta, Family: Chlorodendraceae, Genus: Tetraselmis, Species: Tetraselmis suecica. Golden microalgae can revitalize and regenerate cells, provide antioxidant effects, and soothe the skin.

[0024] Golden microalgae can promote the proliferation of dermal fibroblasts and epidermal keratinocytes, for example, increasing keratinocyte proliferation by 190%; it can also promote the expression of cellular proteins and growth factors, leading to increased synthesis of dermal fibers such as collagen, for example, increasing FBN1 (myofibrillar protein 1) synthesis by 150%; at the same time, it can inhibit the expression of matrix metalloproteinase-1 (an enzyme that degrades fibrin in the matrix) induced by ultraviolet light, reducing its expression level by more than 30%; therefore, golden microalgae can alter the composition of extracellular matrix fibers and the basal layer, helping to strengthen the skin barrier, accelerate epidermal regeneration and repair, and restore skin vitality.

[0025] In addition, golden microalgae possesses excellent antioxidant capabilities. It can neutralize free radicals, inhibit lipid oxidation, and provide direct exogenous antioxidant protection. Simultaneously, it can promote the gene expression of antioxidant enzymes such as SOD, achieving endogenous antioxidant effects. The antioxidant effects of golden microalgae help reduce the production of free radicals, protecting cells from oxidative damage and thus protecting the skin from adverse reactions caused by oxidation, such as allergies, aging, roughness, and redness.

[0026] Meanwhile, golden microalgae also has significant anti-inflammatory activity, which can relieve the irritation caused by stress and environmental attacks, reduce the release of major inflammatory factors, thereby reducing the inflammatory response and soothing the skin. For example, golden microalgae can reduce the release of TNF-α by more than 8% and reduce the release rate of IL-6 by more than 14%.

[0027] Yeast / rice fermentation products are made from high-quality rice from premium producing areas. The fermentation process is carried out under strict control of the fermentation conditions and using a suitable yeast strain (Saccharomyces veronae). Yeast / rice fermentation products contain more than 60 active ingredients, including α-KG, adenosine, amino acids, polysaccharides, and minerals.

[0028] Yeast / rice ferment products have excellent diffusion and penetration properties in skincare products, allowing them to quickly penetrate deep into the skin and provide long-lasting moisturizing effects. The highly effective hydrating capabilities of yeast / rice ferment products help the skin retain moisture and effectively prevent water evaporation.

[0029] α-KG in yeast / rice fermentation products can promote the proliferation of keratinocytes, increase the mRNA expression of skin water retention and barrier-related genes in keratinocytes, such as FLG, SPT, and IVL. FLG is a keratinocyte fibronectin that helps to strengthen the cell wall structure and improve skin barrier function. SPT is a key enzyme in the synthesis of sphingolipids, which can increase the synthesis of sphingolipids in the skin, effectively retaining moisture and resisting external damage. IVL is a precursor of the epidermal keratinization capsule, and its increased expression indicates increased adhesion of the extracellular matrix to the cytoskeleton structure, i.e., enhanced skin barrier function. Therefore, yeast / rice fermentation products can soothe and strengthen the skin barrier.

[0030] In addition, adenosine in yeast / rice fermentation products can provide energy for cells, inhibit the formation of oxygen free radicals, promote skin cell metabolism, and inhibit MMP1 synthesis, thereby stimulating the synthesis of collagen and elastin fibers, enhancing skin firmness and elasticity, and helping to reduce the appearance of fine lines and wrinkles.

[0031] Guaiac is a plant extract. Its pure form is naturally blue. It is a crystalline hydrocarbon and chamomile ring derivative. It is lipid-soluble and mainly exists in plants of the Asteraceae family such as chamomile and chamomile in nature. It is stable under normal conditions, but unstable under light, heat, and pH changes. It will change color if exposed to light for a long time.

[0032] Guaifenesin possesses anti-inflammatory properties and promotes granulation tissue regeneration, thus accelerating the healing of burns and scalds, promoting mucosal wound repair, and preventing chapped skin. It also has anti-inflammatory and soothing properties, relieving skin irritation and allergic reactions to other substances, and can be used to prevent or treat sunburns. Some studies speculate that its excellent anti-inflammatory properties are related to its high lipid solubility and good cell membrane affinity, facilitating its entry into cells to exert its effects. This is highly advantageous for its application in skincare products to exert anti-inflammatory and repairing effects. Furthermore, guaiacine also has analgesic, local anesthetic, and antihistamine effects, acting directly on inflamed tissue cells to inhibit histamine release.

[0033] In traditional technologies, there are relatively few research results on the application of guaiac in the field of skin care products. It is mostly used in toothpaste, mouthwash, oral medications, anti-inflammatory, anti-ulcer, and anti-cracking drugs, and adjuvant drugs for cancer treatment, all of which have been proven to have good efficacy and non-toxicity.

[0034] This application provides a skincare composition comprising golden microalgae, yeast / rice fermentation product, and guaiac. The golden microalgae promotes regeneration and brightening, the yeast / rice fermentation product moisturizes and brightens, and guaiac has anti-inflammatory and regenerative effects. The combination of these three ingredients synergistically enhances skin cell metabolism and microcirculation, thereby reducing redness, swelling, soothing, and repairing the skin. The composition also exhibits good anti-inflammatory and antioxidant activity, comprehensively reducing inflammatory responses, promoting skin barrier repair, and preventing pigmentation. Furthermore, the golden microalgae, yeast / rice fermentation product, and guaiac are all natural extracts, possessing excellent skincare benefits while being gentle and non-irritating, making them suitable for fragile and sensitive skin after cosmetic procedures. This fulfills the need for natural, low-irritation, highly safe, and redness-reducing skincare products after cosmetic procedures.

[0035] In one embodiment, the mass ratio of golden microalgae, yeast / rice fermentation product, and guaiac is (0.1~10):(0.1~10):(0.01~0.25). Within this mass ratio range, the skincare composition can exert better anti-inflammatory, antioxidant, repairing, redness-reducing, and soothing multiple skincare effects. It is understood that the specific mass of the three components (golden microalgae, yeast / rice fermentation product, and guaiac) is not particularly limited, and any component meeting the above-mentioned mass ratio range should be considered within the scope of protection of this application. Further, the mass ratio of golden microalgae, yeast / rice fermentation product, and guaiac is (0.5~8):(0.5~8):(0.05~0.2). Even further, the mass ratio of golden microalgae, yeast / rice fermentation product, and guaiac is 1:1:0.1.

[0036] One embodiment of this application provides a liposome with skin care effects, comprising a lecithin membrane and a skin care effect composition as described in any of the above embodiments, wherein the lecithin membrane encapsulates the skin care effect composition.

[0037] Liposome encapsulation technology is a common drug delivery method used in the pharmaceutical field, effectively enhancing the transdermal absorption of drugs. Lecithin membranes are natural carriers that can encapsulate active ingredients and assist them in penetrating the skin barrier for better absorption. The membrane structure of liposomes is similar to that of cell membranes, exhibiting strong cell affinity and allowing them to fuse with cell membranes. This enables the release of encapsulated active ingredients into the cell, or allows the entire liposome to be phagocytosed by the cell, achieving full absorption of the active ingredients within the cell.

[0038] Applying liposome encapsulation technology to the aforementioned skincare composition creates a stable complex that enhances the synergistic effect of golden microalgae, yeast / rice fermentation products, and guaiac, resulting in superior skincare efficacy. Specifically, liposome encapsulation technology addresses the issues of active ingredient inactivation and poor stability. Furthermore, the sustained-release nature of liposomes allows for control over the release rate of the active ingredients in the composition, enabling a gradual release of these three active ingredients onto the skin. This increases the efficacy, longevity, and safety of the skincare product. Simultaneously, liposome encapsulation technology improves the transdermal absorption of the active ingredients, making the product safer, gentler, and with higher absorption efficiency of functional components.

[0039] In one embodiment, the composition with skin-care effects comprises 60% to 70% of the sum of the mass of the lecithin membrane and the composition with skin-care effects. Within this percentage range, the composition can be encapsulated by the lecithin membrane to form stable liposomes without affecting the realization of the multiple skin-care effects of the golden microalgae, yeast / rice fermentation products, and guaiac in the composition. Preferably, the composition with skin-care effects comprises 60% of the sum of the mass of the lecithin membrane and the composition with skin-care effects.

[0040] In one embodiment, the liposomes have a particle size of 0.1 micrometers to 1 micrometer. Liposomes within this particle size range are more easily penetrated and absorbed by the skin, resulting in better skincare effects after use. Furthermore, liposomes within this particle size range are easier to mix evenly with other ingredients in skincare products, leading to higher performance stability in the resulting creams, lotions, and other skincare products.

[0041] One embodiment of this application provides a method for preparing liposomes with skin care effects as described in any of the above embodiments, including the following steps S110 to S130.

[0042] Step S110: Provide a lecithin membrane.

[0043] Understandably, in this application, lecithin membranes can be obtained by purchasing them externally or by conventional methods in the art, and are not specifically limited in this application.

[0044] In one embodiment, the lecithin membrane is prepared by including the following steps:

[0045] Lecithin is dissolved in ethanol to form a lecithin ethanol solution. The lecithin ethanol solution is then transferred to a rotary evaporator to evaporate the ethanol. After the ethanol evaporates, a uniform lecithin film is formed on the container wall of the rotary evaporator.

[0046] Step S120: Mix the skin-care composition with a phosphate buffer solution to form a mixed solution.

[0047] In one embodiment, the pH of the phosphate buffer solution is 5.5 to 8.5. The phosphate buffer solution allows for thorough mixing of the components in a skin-care-enhancing composition.

[0048] Step S130: Add the mixed solution to a container containing a lecithin membrane, and perform hydration and sonication treatment.

[0049] In one embodiment, the hydration treatment conditions include a temperature of 30°C to 80°C and a time of 25 to 35 minutes. Hydration under these specific conditions allows the lecithin membrane to completely detach from the container wall and fully mix with the solution.

[0050] In one embodiment, the ultrasonic treatment conditions include a power of 600W~800W and a time of 3.5min~4.5min. Through ultrasonic treatment under specific conditions, the golden microalgae, yeast / rice fermentation products, and guaiac in the mixed solution can be fully encapsulated by the lecithin membrane, forming liposomes with stable performance and structure.

[0051] An embodiment of this application also provides the use of the skin-care effect composition of any of the above embodiments or the skin-care effect liposome of any of the above embodiments in the preparation of skin-care products.

[0052] An embodiment of this application also provides a skin care product, the raw materials of which include the skin care effect composition or the skin care effect liposomes in any of the above embodiments.

[0053] The skincare products provided in this application can achieve the following multiple effects:

[0054] 1. Deeply and multidimensionally reduces skin redness, soothes and repairs the skin.

[0055] Antioxidant: Golden microalgae, yeast / rice fermentation products and guaiac can all inhibit the production of oxygen free radicals. Golden microalgae can also neutralize and eliminate free radicals, preventing a series of skin problems caused by oxidation, such as inflammation, aging, roughness, redness and swelling.

[0056] Anti-inflammatory: Both golden microalgae and guaiac have significant anti-inflammatory factor expression effects, which can reduce inflammatory response, prevent excessive reaction of tissues and skin, and soothe the skin.

[0057] Enhancement: The three substances—golden microalgae, yeast / rice fermentation products, and guaiac—all promote epidermal cell proliferation, enhance the expression of cellular matrix components such as fibrin and elastin, and inhibit the expression of MMP-1, an enzyme that degrades matrix proteins, thus comprehensively strengthening the skin barrier. Furthermore, the synergistic effect of these three substances can improve cellular metabolic activity, enhance cellular energy, strengthen microcirculation to provide sufficient nutrition, promote tissue regeneration and the healing of micro-wounds, achieving wound repair and reducing redness and swelling.

[0058] 2. The raw materials have clear efficacy and value, are natural and mild, and have high safety.

[0059] Traditional soothing and repairing skincare products and oral medications mostly use sodium guaiacum sulfonate, a derivative of guaiacum, as a raw material. While sodium guaiacum sulfonate is water-soluble and has better compatibility, it is chemically unstable and decomposes under light, air oxidation, and high temperatures, easily losing its sulfonic acid groups. Furthermore, the preparation process requires chemical reactions using guaiacum as a raw material, resulting in poor safety and complex processes. This application uses guaiacum extracted directly from pure natural plants as one of the raw materials for skincare products, combined with other natural extracts such as golden microalgae and yeast / rice fermentation products. The product exhibits good bioactivity, is suitable for sensitive skin, and better meets the natural and gentle needs of post-medical aesthetic repair skincare products.

[0060] 3. Enhances the permeability of raw materials from multiple dimensions, allowing them to penetrate deep into the skin and exert their effects.

[0061] Golden microalgae, yeast / rice fermentation products, and guaiac all have good biocompatibility and permeability, and also have a synergistic effect. Furthermore, liposome encapsulation technology is used to further promote the more efficient penetration of active ingredients into the deep layers of the skin, and to achieve sustained-release and long-lasting efficacy.

[0062] Understandably, the skincare products provided in this application may be conventional skincare products in the field, such as face creams, lotions, etc.

[0063] Furthermore, the skincare product is a face cream. In addition to the skincare-enhancing compositions or liposomes mentioned above, the ingredients of the face cream may also include other ingredients commonly found in the art, such as any one or more combinations of solvents, skincare agents, thickeners, chelating agents, pH adjusters, preservatives, and fragrances.

[0064] Furthermore, other raw materials may include, but are not limited to, water, sodium acrylate / sodium acryloyl dimethyl taurate copolymer, xanthan gum, carboxymethyl cellulose, phenoxyethanol, hexanediol, EDTA-2NA, kaolin, titanium dioxide, avocado oil, squalane, tocopheryl acetate, behenol, isononyl isononanoate, PEG-100 stearate, fragrances, etc.

[0065] Understandably, the skincare products provided in this application can be prepared using any conventional preparation method in the art.

[0066] In one embodiment, the skincare product is a face cream, and the ingredients include:

[0067] Phase A: Water, sodium acrylate / sodium acryloyl dimethyl taurate copolymer, xanthan gum, carboxymethyl cellulose, phenoxyethanol, hexanediol, EDTA-2NA;

[0068] Phase B: Kaolin, titanium dioxide;

[0069] Phase C: Avocado oil, squalane, tocopheryl acetate, behenyl alcohol, isononyl isononanoate;

[0070] Phase D: PEG-100 stearate, daily fragrance;

[0071] E phase: The composition or liposome with skin care effect provided in any of the above embodiments;

[0072] The preparation method of face cream includes the following steps S210 to S230.

[0073] Step S210: Mix and completely disperse the raw materials of phase A to obtain a mixture;

[0074] Step S220: Add the raw materials from phases B, C, and E to the mixture, heat, and homogenize and disperse.

[0075] Step S230: Add the raw materials of phase D to the product of step S220 and homogenize and emulsify.

[0076] The following detailed embodiments further illustrate the skincare composition, skincare liposomes, their preparation methods, and applications of this application. While the following embodiments are specific, it is understood that other embodiments are not limited to these examples. Unless otherwise specified, the instruments, reagents, and materials involved in the following specific embodiments are all conventional instruments, reagents, and materials already available in the prior art and can be obtained through legitimate commercial channels. Unless otherwise specified, the experimental methods and detection methods involved in the following embodiments are all conventional experimental methods and detection methods already available in the prior art.

[0077] Golden Microalgae: Manufacturer: Weidan Biotechnology Co., Ltd.

[0078] Guaifenesin: The manufacturer is Huzhou Purui Biomedical Technology Co., Ltd.

[0079] Yeast / rice fermentation products are prepared by the following method:

[0080] (1) Selected yeast strains were inoculated into sterilized activation medium and cultured at 28°C for 48 hours to prepare activated strains for later use.

[0081] (2) Under aseptic conditions, the activated strain prepared in step (1) is transferred to the sterilized expansion medium using an inoculation loop, placed in a constant temperature shaker, and cultured for 48 hours at 150 r / min and 28℃ to prepare the expanded strain.

[0082] (3) Inoculate the expanded strain prepared in step (2) into MRS medium with an inoculation amount of 3% and continue to culture at 28°C for 48 hours to prepare a strain suspension.

[0083] (4) Wash 20g of selected rice with water, soak for 24 hours, take it out and steam it in a pot, put it in a bottle, add deionized water and let it stand and cool to room temperature, while making the rice grains loose, to prepare cooked rice.

[0084] (5) Take 15% of the mass of the rice grains prepared in step (4) and add the bacterial suspension prepared in step (3) to the rice grains. Cultivate and ferment at 37°C for 2 days to prepare fermentation liquid.

[0085] (6) The fermentation broth prepared in step (5) is allowed to stand, filtered, and the clarified liquid is collected to obtain yeast / rice fermentation product.

[0086] Example 1

[0087] (1) Preparation of liposomes with skin care effects:

[0088] 1200 mg of lecithin was dissolved in 15 mL of ethanol and stirred until fully dissolved. The solution was then transferred to a rotary evaporator and slowly evaporated in a 65°C water bath, forming a uniform lecithin film on the container wall. 25 mL of a pH 7.4 phosphate buffer solution was mixed with 858 mg of yeast / rice fermentation product filtrate, 858 mg of golden microalgae, and 85.8 mg of guaiac. The mixture was then transferred to the rotary evaporator container for hydration treatment of the lecithin film for 20 min at 50°C. After complete film detachment, the film was sonicated for 4 minutes at a power of 700 W to obtain liposomes with skin-care effects. The liposomes had a particle size of 0.11 μm. The mass ratio of golden microalgae, yeast / rice fermentation product, and guaiac in the skin-care effect composition was 10:10:1. The mass of the skin-care effect composition accounted for 60% of the sum of the mass of the lecithin film (equal to the weight of lecithin) and the mass of the skin-care effect composition.

[0089] (2) Composition of face cream ingredients:

[0090] The ingredients and percentage by weight of the face cream are shown in Table 1 below.

[0091] Table 1. Ingredients and weight percentages of the face cream in Example 1

[0092]

[0093] The raw material of phase E comes from the liposomes with skin care effect prepared in step (1). The amount of liposomes with skin care effect added is 3.5% of the mass of the face cream raw material. The mass percentages of golden microalgae, yeast / rice fermentation product and guaiac are 1%, 1% and 0.1% of the mass of the face cream raw material, respectively, and the remaining 1.4% is lecithin membrane.

[0094] (3) Preparation of face cream:

[0095] (I) Wet and disperse phase A in deionized water to obtain a mixed solution;

[0096] (II) Add phases B, C and E from Table 1 to the mixture in step (I), heat to 60°C, and homogenize at 3000 r / min for 4 minutes to ensure complete dispersion;

[0097] (III) Add phase D to step (II) and homogenize it at 3000 r / min for 3 minutes to emulsify it completely to obtain face cream.

[0098] Example 2

[0099] (1) Preparation of liposomes with skin care effects:

[0100] The steps are largely the same as in Example 1 (1), except that the mass ratio of golden microalgae, yeast / rice fermentation product and guaiac in the composition with skin care effect is 8:8:0.2, and the mass of the composition with skin care effect accounts for 60% of the sum of the mass of lecithin membrane (equal to the weight of lecithin) and the mass of the composition with skin care effect.

[0101] (2) Composition of face cream ingredients:

[0102] The steps are largely the same as in Example 1 (2), except that:

[0103] The raw material of phase E comes from the liposomes with skin care effect prepared in step (1). The amount of liposomes with skin care effect added is 27% of the mass of the face cream raw material. Among them, the mass percentages of golden microalgae, yeast / rice fermentation product and guaiac are 8%, 8% and 0.2% of the mass of the face cream raw material, respectively, and the remaining 10.8% is lecithin membrane.

[0104] (3) Preparation of face cream:

[0105] The same as step (3) in Example 1.

[0106] Example 3

[0107] (1) Preparation of liposomes with skin care effects:

[0108] The same as step (1) in Example 1.

[0109] (2) Composition of face cream ingredients:

[0110] The steps are largely the same as in Example 1 (2), except that:

[0111] The mass percentage of liposomes with skin care effects in the face cream raw materials is 1.75%, of which the mass percentages of golden microalgae, yeast / rice fermentation products and guaiac are 0.5%, 0.5% and 0.05% respectively, and the remaining 0.7% is lecithin membrane.

[0112] (3) Preparation of face cream:

[0113] The same as step (3) in Example 1.

[0114] Example 4

[0115] Similar to Example 1, except that the skin-care-enhancing composition of golden microalgae, yeast / rice fermentation products, and guaiac was not encapsulated in a lecithin membrane to form liposomes. The cream was prepared as follows:

[0116] (1) Composition of face cream ingredients:

[0117] The ingredients and percentages by weight of the face cream are shown in Table 2 below.

[0118] Table 2. Ingredients and weight percentages of face cream in Example 4

[0119]

[0120] (2) Preparation of face cream:

[0121] (I) Wet and disperse phase A in deionized water to obtain a mixed solution;

[0122] (II) Add phases B, C and E from Table 1 to the mixture in step (I), heat to 60°C, and homogenize at 3000 r / min for 4 minutes to ensure complete dispersion;

[0123] (III) Add phase D to step (II) and homogenize it at 3000 r / min for 3 minutes to emulsify it completely to obtain face cream.

[0124] Comparative Example 1

[0125] Similar to Example 4, except that the percentages of the ingredients in the cream are as follows: golden microalgae, yeast / rice fermentation product and guaiac are 1.9%, 0%, and 0.2%, respectively.

[0126] Comparative Example 2

[0127] Similar to Example 4, except that the percentages of the ingredients in the cream are: 0%, 1.9%, and 0.2%, respectively.

[0128] Comparative Example 3

[0129] Similar to Example 4, except that the percentages of the ingredients in the cream are as follows: golden microalgae, yeast / rice fermentation product and guaiac are 1.05%, 1.05% and 0%, respectively.

[0130] Comparative Example 4

[0131] Similar to Example 4, except that the percentages of the ingredients in the cream are: 0%, 0%, and 2.1%, respectively.

[0132] Comparative Example 5

[0133] Similar to Example 4, except that the percentages of the ingredients in the cream are: 0%, 2.1%, and 0%, respectively.

[0134] Comparative Example 6

[0135] Similar to Example 4, except that the percentages of the ingredients in the cream are as follows: 2.1%, 0%, and 0%, respectively.

[0136] Inhibition of the inflammatory cytokine IL-6 (interleukin-6) was tested in Examples 1-4:

[0137] Normal human epidermal keratinocytes (NHEK) were cultured in a culture medium. After adhesion (24 hours after inoculation), the cells were treated with the corresponding drugs for 7 days according to the following methods: blank control group, negative control group 1, positive control group 1, and experimental groups 1-4. The corresponding tests were then performed.

[0138] Blank control group: Cells that have not undergone any treatment.

[0139] Negative control group 1: Lipopolysaccharide (LPS, 500 ng / ml, 50 mL) was applied to NHEK cells to induce an inflammatory response. Lipopolysaccharide is a major component of the exowall of pathogenic Gram-negative bacteria and can stimulate giant cells to produce pro-inflammatory factors to promote the inflammatory response.

[0140] Positive control group 1: After inducing an inflammatory response by applying lipopolysaccharide (LPS, 500 ng / ml, 50 mL) to NHEK cells, dexamethasone was administered. Dexamethasone is a synthetic corticosteroid that is effective in reducing inflammation.

[0141] Experimental groups 1-4: Each experimental group first applied lipopolysaccharide (LPS, 500 ng / ml, 50 mL) to NHEK cells to induce an inflammatory response, and then applied the face cream (0.5 g) prepared in Examples 1-4 respectively.

[0142] Seven days after administration, the concentrations of inflammatory factors in each group (n=10) were detected using an IL-6 ELISA kit. Samples were processed and prepared strictly according to the kit instructions. The wavelength of the microplate reader was adjusted to 368 nm, and the absorbance values ​​of each group of samples were measured and the concentrations were calculated.

[0143] Compared with the blank control group, the IL-6 secretion level in the negative control group 1 was significantly increased (P<0.01), indicating that the application of lipopolysaccharide to cells can effectively stimulate the production of inflammation.

[0144] Then, the rate of change in inflammatory factor release in the positive control group 1 and experimental groups 1-4 relative to the negative control group 1 was calculated. The rate of change in inflammatory factor release was calculated as follows: (concentration of inflammatory factors in the positive control group 1 or experimental groups 1-4 - concentration of inflammatory factors in the negative control group 1) ÷ concentration of inflammatory factors in the negative control group × 100%. The test results are shown in Table 3 below.

[0145] Table 3 Results of the IL-6 Inhibition Test

[0146]

[0147] In the positive control group 1, after inflammation was induced, dexamethasone was applied. The rate of change in inflammatory factor release was significantly lower compared to the negative control group 1, indicating that dexamethasone is a drug with good anti-inflammatory effects. The closer the experimental results of experimental groups 1-4 are to the experimental results of the positive control group 1, the better the anti-inflammatory effect of that experimental group. It can be seen that in experimental groups 1-4, after applying the creams of Examples 1-4 respectively, the release of inflammatory factors in NHEK cells was significantly lower than that of the negative control group 1, indicating that the creams of Examples 1-4 can all play a certain anti-inflammatory role. Simultaneously, the anti-inflammatory effect was: dexamethasone > cream of Example 2 > cream of Example 1 > cream of Example 4 > cream of Example 3.

[0148] The antioxidant capacity of Examples 1 to 4 was tested using the DPPH free radical scavenging method:

[0149] Negative control group 2: Weigh 0.0078g of DPPH, dissolve it in anhydrous ethanol, and make up to 100mL to obtain a DPPH solution with a concentration of 0.2mmol / L.

[0150] Positive control group 2: Accurately weigh 5 mg of vitamin C powder, dissolve it in distilled water, and bring the volume to 100 mL. Then, take 1 mL of the vitamin C solution and place it in a test tube, and add distilled water to bring the volume to 2 mL. Add 3 mL of negative control DPPH solution, and react under light-protected conditions for 30 minutes. Ascorbic acid is an endogenous antioxidant with significant antioxidant effects.

[0151] Experimental groups 5-8: After generating a large amount of reactive oxygen species DPPH using ultraviolet light, the face creams (2g) of Examples 1-4 were applied for 24 hours respectively.

[0152] DPPH is a stable nitrogen-centered free radical. Its ethanol solution is purple, with a maximum absorption wavelength of 517 nm. When a free radical scavenger is added to the DPPH solution, its unpaired electrons are paired, the solution color lightens to yellow or pale yellow, and the absorbance at 517 nm decreases. The degree of absorbance change is linearly related to the degree of free radical scavenging. Under the same experimental conditions, after co-treatment with DPPH for 0.5 h, the absorbance of each group was measured, and the free radical scavenging rate was calculated. Free radical scavenging rate = (A... 阴性对照组2 -A 阳性对照组2或实验组5~8 ) / A 阴性对照组2 The results are shown in Table 4 below.

[0153] Table 4. Free radical scavenging rate relative to negative control group 2

[0154]

[0155] As shown in Table 4, the DPPH content of free radicals in the positive control group 2 decreased significantly, the free radical scavenging rate was high, and the antioxidant effect was outstanding.

[0156] In experimental groups 5-8, after applying the face creams from Examples 1-4, the DPPH content of free radicals significantly decreased compared to the negative control group 2, indicating that the face creams from Examples 1-4 all had a certain effect in scavenging free radicals and anti-oxidation. The closer the experimental results of experimental groups 5-8 were to the experimental results of the positive control group 2, the better the antioxidant effect of that group. Therefore, the antioxidant effect was: ascorbic acid > face cream of Example 2 > face cream of Example 1 > face cream of Example 4 > face cream of Example 3.

[0157] It can be observed that the antioxidant effects of the face creams in Examples 1 to 4 are ranked in the same order as the aforementioned anti-inflammatory effects. Furthermore, the antioxidant and anti-inflammatory effects of Example 1 are superior to those of Example 4, and the effects of Example 1 are superior to those of Examples 2 to 4. This indicates that in face creams, the higher the content of the composition or liposomes composed of golden microalgae, yeast / rice fermentation products, and guaiac, the better the antioxidant and anti-inflammatory effects of the face cream. Moreover, under the premise that the percentage concentrations of golden microalgae, yeast / rice fermentation products, and guaiac are the same, encapsulating the composition into liposomes with a lecithin membrane further enhances the antioxidant and anti-inflammatory capabilities of the face cream.

[0158] The skin repair efficacy of the face creams prepared in Examples 1-4 and Comparative Examples 1-6 was tested.

[0159] Thirty healthy volunteers, aged 18-30 years with no history of allergies, were selected and randomly divided into 6 groups of 5. A half-face test was conducted using a randomization table, selecting the left and right cheeks for TEWL (transient moisture loss) testing. Volunteers used the product as instructed, once in the morning and once in the evening, for 56 consecutive days. Follow-up tests were conducted on days 7, 28, and 56 of the experiment.

[0160] Transepidermal water loss (TEWL) was accurately measured using the non-invasive measurement device TEWL (Dermalab Combo), with units of g / m³. 2 The test measured the change in transepidermal water loss (TEWL) before and after product use. A significant decrease in TEWL indicates improved water loss and restoration of the skin barrier, suggesting the cream product has skin-repairing effects. The test results are shown in Table 5 below.

[0161] TEWL skin transepidermal water loss rate (%) = (TEWL skin transepidermal water loss after applying face cream - TEWL skin transepidermal water loss before applying face cream) ÷ TEWL skin transepidermal water loss before applying face cream × 100%.

[0162] Table 5. Change rate of transepidermal water loss value in TEWL skin

[0163]

[0164] As shown in Table 5, after 56 days of consistent use of the face cream, compared to Comparative Examples 1-6, the TEWL transepidermal water loss values ​​of Examples 1-4 were lower, indicating a significant improvement in skin hydration. This suggests that the skin barrier was restored, demonstrating that the face creams of Examples 1-4 have skin repair effects. Among them, Example 2 showed the best repair effect. Furthermore, a comparison between Example 4 and Comparative Examples 1-6 shows that when the composition simultaneously contains golden microalgae, yeast / rice fermentation products, and guaiac, the three components exert a synergistic effect, significantly improving skin repair efficacy. When only one or two of the above three components are present, the skin repair effect is poor.

[0165] The skin redness-reducing efficacy of the face creams prepared in Examples 1-4 and Comparative Examples 1-6 was tested.

[0166] Thirty healthy volunteers, aged 20-30 years with no history of allergies, were selected and randomly divided into 6 groups of 5 volunteers each. A full-face test was conducted according to a randomization table, and skin color was tested on the cheeks of the volunteers. The volunteers used the product as instructed, once in the morning and once in the evening, for 56 consecutive days. Follow-up tests were conducted on days 7, 28, and 56 of the experiment.

[0167] The skin redness-reducing effect was analyzed by measuring changes in the a* value of skin color (VISIA & IPP) (cheeks). Facial images were captured using VISIA and analyzed using Image-Pro skin analysis software. A lower a* value indicated improved skin redness. The test results are shown in Table 6 below.

[0168] Skin color a* value change rate (%) = (skin color a* value after applying face cream - skin color a* value before applying face cream) ÷ skin color a* value before applying face cream × 100%.

[0169] Table 6. Rate of change of skin color a* value

[0170]

[0171] As shown in Table 6, after 56 days of consistent use of the face cream, compared to Comparative Examples 1-6, the smaller the skin color a* value of Examples 1-4, the more significant the improvement in skin redness, indicating that the face cream products of Examples 1-4 have the effect of reducing skin redness. Among them, Example 2 showed the best effect in reducing redness. In addition, the comparison between Example 4 and Comparative Examples 1-6 shows that when the composition contains golden microalgae, yeast / rice fermentation products, and guaiac, long-term use can result in a synergistic effect of the three components, significantly improving the skin redness reduction effect. When only one or two of the above three components are contained, the skin redness reduction effect is poor.

[0172] The skin-soothing efficacy of the face creams prepared in Examples 1-4 and Comparative Examples 1-6 was tested using a lactic acid stinging test.

[0173] The lactic acid stinging test method is as follows: At room temperature, apply 50 μL of a 10% lactic acid solution to the nasolabial fold and one cheek. Inquire about the subject's subjective symptoms at 2.5 min and 5 min, respectively, using a 4-point scale (0 points for no stinging, 1 point for mild stinging, 2 points for moderate stinging, and 3 points for severe stinging). Then, add the two scores together; a total score >3 points indicates a positive lactic acid stinging reaction. The same lactic acid stinging test was performed after 14 days and 28 days of continuous use of the example.

[0174] This study included 100 healthy female volunteers (10 per group) aged 18-45 years from Shanghai, who reported having sensitive skin. Symptoms included mild facial redness, telangiectasia, erythema, dryness (flaking, tightness), and a lactic acid stimulation test score >3. Participants strictly adhered to medication instructions and underwent regular checkups during the study. They had no history of skin diseases or other serious illnesses within the past three months and had not taken any antibiotics or hormonal medications. Written informed consent was provided to all participants before the study began, and they signed the consent forms after thorough communication. This study protocol was approved by the Fudun Ethics Committee, and the entire study was subject to strict supervision and guidance from the committee. The study strictly protected the privacy and data security of the participants.

[0175] The lactic acid stinging test was used to evaluate facial skin. If the lactic acid stinging score did not increase significantly after using the face cream, it indicates that the face cream product will not aggravate skin sensitivity and is suitable for sensitive skin. If the lactic acid stinging score decreased significantly after using the face cream, it indicates that the face cream product can alleviate the skin's sensitivity and has a certain soothing effect. Combining clinical and subjective assessments, if the face cream product can significantly reduce stinging, itching, tightness, redness, etc., it indicates that the face cream product has a soothing effect.

[0176] The results of the lactic acid stinging test (the average total score of 2.5 min and 5 min for 10 subjects in each group) are shown in Table 7 below.

[0177] Table 7. Changes in total score of lactic acid sting test over time.

[0178]

[0179] As shown in Table 7, after 28 days of consistent use of the face cream, compared with Comparative Examples 1 to 6, the smaller the total score of the lactic acid stinging test in Examples 1 to 4, the better the skin soothing effect of the face creams in Examples 1 to 4.

[0180] As shown in Tables 5-7, in terms of repair, redness reduction, and soothing effects, Example 2 is the best among Examples 1-3, followed by Example 1, and then Example 3. This indicates that within this range, the higher the concentration of liposomes and their contained compositions in the cream raw materials, the more significant the effect. In Example 4, the content of each component in the cream raw materials is the same as in Example 1, but the components in the composition are not encapsulated and protected by liposomes. Even though the concentration of each component is the same as in Example 1, the effect is still weaker than in Example 1. This indicates that liposome encapsulation technology helps promote the absorption of each component in the composition by the skin, which is more conducive to exerting good efficacy.

[0181] In Tables 5-7, the effects of Comparative Examples 1-6 were not as good as those of Example 1, indicating that the combination of golden microalgae, yeast / rice fermentation products and guaiac has a better moisturizing effect and a synergistic effect.

[0182] The technical features of the above embodiments can be combined in any way. For the sake of brevity, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0183] The embodiments described above are merely illustrative of several implementations of the present invention, and while the descriptions are specific and detailed, they should not be construed as limiting the scope of the invention patent. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of the present invention, and these all fall within the protection scope of this application. Therefore, the protection scope of this patent application should be determined by the appended claims.

Claims

1. A composition with skin care effects, characterized in that, The components include: Golden microalgae, yeast / rice fermentation products, and guaiac; The mass ratio of the golden microalgae, the yeast / rice fermentation product, and the guaiac is (0.1~10):(0.1~10):(0.01~0.25); The gold microalgae is prepared by co-culturing algae extract with gold. The yeast / rice fermentation product was prepared by the following method: Yeast was inoculated into sterilized activation medium and cultured at 28°C for 48 hours to prepare activated strains. Under aseptic conditions, the activated bacterial strain was transferred to the sterilized expansion medium using an inoculation loop, placed in a constant temperature shaker, and cultured for 48 hours at 150 r / min and 28°C to prepare the expanded bacterial strain. The expanded bacterial strain was inoculated into MRS medium at an inoculation amount of 3%, and cultured at 28°C for 48 hours to prepare a bacterial suspension. Wash 20g of rice with water, soak for 24 hours, drain and steam in a pot until cooked, then put into a bottle, add deionized water and let stand and cool to room temperature, while loosening the rice grains to prepare cooked rice. Take 15% of the bacterial suspension by mass of the rice grains and add it to the rice grains. Incubate and ferment at 37°C for 2 days to prepare fermentation broth. The fermentation broth was allowed to stand, filtered, and the clarified liquid was collected to obtain the yeast / rice fermentation product.

2. The composition with skin-care effects according to claim 1, characterized in that, The mass ratio of the golden microalgae, the yeast / rice fermentation product, and the guaiac is (0.5~8):(0.5~8):(0.05~0.2).

3. A liposome with skin-care effects, characterized in that, The composition comprises a lecithin membrane and a skin-care effect composition as described in any one of claims 1 to 2, wherein the lecithin membrane encapsulates the skin-care effect composition.

4. The liposome with skin-care effect according to claim 3, characterized in that, The composition having skin-care effects accounts for 60% to 70% of the sum of the mass of the lecithin membrane and the composition having skin-care effects.

5. The liposome with skin-care effect according to any one of claims 3 to 4, characterized in that, The liposomes have a particle size of 0.1 micrometers to 1 micrometer.

6. A method for preparing liposomes with skin-care effects as described in any one of claims 3 to 5, characterized in that, Includes the following steps: Provide the lecithin membrane; The skin-care-enhancing composition is mixed with a phosphate buffer solution to form a mixed solution; The mixed solution was added to a container containing the lecithin membrane and then subjected to hydration and ultrasonic treatment.

7. The method for preparing liposomes with skin-care effects according to claim 6, characterized in that, The pH value of the phosphate buffer solution is 5.5~8.

5.

8. The method for preparing liposomes with skin-care effects according to any one of claims 6 to 7, characterized in that, During the hydration and ultrasonic treatment steps, at least one of the following conditions must be met: (1) The conditions for hydration treatment include: temperature of 30℃~80℃ and time of 25min~35min; (2) The conditions for ultrasonic treatment include: power of 600W~800W and time of 3.5min~4.5min.

9. The use of the composition with skin care effect according to any one of claims 1 to 2 or the liposome with skin care effect according to any one of claims 3 to 5 in the preparation of skin care products.

10. A skincare product, characterized in that, The raw materials of the skin care product include the composition with skin care effect as described in any one of claims 1 to 2 or the liposome with skin care effect as described in any one of claims 3 to 5.

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