Application of Panax notoginseng root extract in the preparation of daily chemical products

Abstract

This application discloses the application of *Gynostemma pentaphyllum* root extract in the preparation of daily chemical products, relating to the field of daily chemical product technology. This application utilizes *Gynostemma pentaphyllum* root extract as an anti-irritant in daily chemical products, capable of preventing and / or treating skin irritation from irritants. It not only has promising application prospects but also expands the applications of *Gynostemma pentaphyllum* root extract.

Description

Technical Field

[0001] This application relates to the field of daily chemical product technology, specifically to the application of Panax notoginseng root extract in the preparation of daily chemical products. Background Technology

[0002] Gymnadenia conopsea (L.) R.Br., also known as palm ginseng, hand ginseng, or Buddha's palm ginseng, is the tuberous root of the orchid Gymnadenia conopsea or Gymnadenia thunbergii. Rich in various physiologically active substances, it is reported to contain approximately 30 wt% polysaccharides, 27 wt% starch, 20 wt% protein, and 5 wt% reducing sugars, as well as calcium oxalate, glycosides, and trace elements, giving it high edible and medicinal value. In dietary therapy and health maintenance, Gymnadenia conopsea is used to nourish and invigorate the kidneys, and is used for spermatorrhea, premature ejaculation, impotence, emaciation, weakness due to prolonged illness, and mental confusion. It can be directly boiled into soup, stewed with chicken, soaked in alcohol, or taken with milk. In terms of disease treatment, *Gynostemma pentaphyllum* has the effects of relieving cough and asthma; tonifying the kidneys and spleen; regulating qi and blood; relieving pain; replenishing qi and blood, promoting body fluid and quenching thirst; and treating lung deficiency cough and asthma, consumptive emaciation, neurasthenia, chronic diarrhea, blood loss, leukorrhea, insufficient lactation, and chronic hepatitis. *Jingzhu Materia Medica* records: "*Gynostemma pentaphyllum* promotes sperm production and strengthens yang, increases physical strength, greatly replenishes vital energy, calms the mind, enhances intelligence, and prolongs life. However, it is rare and difficult to obtain. Although its medicinal properties are mild, its effects are long-lasting."

[0003] Hand-shaped ginseng, as a Tibetan medicine, is generally used in the combination of traditional Chinese and Tibetan medicines, primarily for internal use, such as in Hand-shaped Ginseng Kidney-Treasure Capsules, Compound Hand-shaped Ginseng Intelligence-Boosting Capsules, and Ten-Flavor Hand-shaped Ginseng Powder. However, research on the use of Hand-shaped Ginseng root extract as a topical skin preparation is scarce, and research on its anti-irritant effects is even less. Summary of the Invention

[0004] Given the deficiencies in the existing technology, the purpose of this application is to provide the application of Panax notoginseng root extract in the preparation of daily chemical products, using Panax notoginseng root extract as an anti-irritant to prevent and / or treat skin irritation caused by irritants.

[0005] To achieve the above objectives, this application provides the use of Panax notoginseng root extract as an anti-irritant in the preparation of daily chemical products, wherein the Panax notoginseng root extract can prevent and / or treat skin irritation caused by irritants in daily chemical products.

[0006] During their research, the inventors unexpectedly discovered that the root extract of *Gynostemma pentaphyllum* possesses excellent anti-irritant properties and can be used to prevent and / or treat skin irritation caused by irritants. For example, the root extract can inhibit skin irritation caused by chemical factors and / or treat skin irritation induced by chemical reagents, including at least one of SLS (sodium lauryl sulfate) and methyl nicotinic acid, but not limited to these. The root extract can also inhibit skin irritation caused by physical factors and / or treat skin irritation induced by physical factors, such as inhibiting skin irritation induced by hot water (e.g., 40–65°C) and UVA / UVB / sun exposure. Furthermore, the root extract can inhibit skin irritation caused by bacteria and / or treat bacterial-induced skin irritation, such as inhibiting skin irritation caused by bacterial toxins and / or treating skin irritation induced by bacterial toxins.

[0007] In one embodiment, the irritant includes at least one of chemical factors, physical factors, bacteria, and viruses.

[0008] In one embodiment, the irritant includes at least one of sodium lauryl sulfate, methyl nicotinic acid, hot water, UVA, and UVB.

[0009] In one embodiment, the mass fraction of *Gynostemma pentaphyllum* root extract in the daily chemical product is 0.009% to 11%, such as 0.01%, 0.05%, 0.1%, 0.5%, 1%, 3%, 5%, 7%, 9%, 10%, etc., which can effectively prevent and / or treat skin irritation caused by irritants. Especially when the mass fraction of *Gynostemma pentaphyllum* root extract in the daily chemical product is 0.05% or 0.5% to 0.6%, the effect of preventing and / or treating skin irritation caused by irritants is even better.

[0010] In one embodiment, the preparation method of the palm-shaped ginseng root extract includes the following steps:

[0011] Wash the root of the palm ginseng with water;

[0012] Mix the cleaned ginseng root with pure water and heat it until there is no white core left.

[0013] The soaked rootlets of the palm-shaped ginseng were crushed to obtain a dispersed slurry;

[0014] Pure water was added to the dispersion slurry for dilution, then neutral protease was added, and the mixture was circulated and homogenized for extraction to obtain the extraction solution.

[0015] The enzymes in the extracted liquid are inactivated by heating.

[0016] The enzyme-inactivated extract is then subjected to coarse and fine filtration to obtain a clear and transparent solution.

[0017] The resulting clear and transparent solution was concentrated to obtain a concentrated solution;

[0018] The concentrate is dried to obtain the palm root extract.

[0019] In one embodiment, at least one of the following conditions is satisfied:

[0020] a) During the heating and soaking process, the ratio of ginseng root to pure water is ginseng root: pure water = 1 kg: 40 L, and the heating and soaking temperature is 40 °C.

[0021] b) The particle size of the solid particles in the dispersion slurry is 60 mesh;

[0022] c) After the dilution, the ratio of ginseng tuber to pure water is ginseng tuber: pure water = 1 kg: 40 L;

[0023] d) The mass of the neutral protease is 0.01% of the mass of the root tuber of the palm-shaped ginseng;

[0024] e) The particle size of the solid particles in the extraction liquid is 100 mesh;

[0025] f) The temperature for inactivating the enzyme by heating is 75°C.

[0026] In one embodiment, during the heating and soaking process, the temperature is raised to the target temperature while stirring, then stirring is stopped, and the soaking is maintained at the target temperature. This stirring process helps to accelerate the soaking process.

[0027] In one embodiment, the drying includes at least one of heating drying, vacuum drying, freeze drying, etc., such as drying in at least one of the following devices: hot air circulating oven, vacuum drying oven, freeze dryer, etc.

[0028] The daily chemical products can be aqueous solutions, lotions, creams, or other dosage forms. In one embodiment, the daily chemical products include at least one of toothpaste, mouthwash, soap, shampoo, shower gel, dishwashing liquid, laundry detergent, laundry liquid, facial cleanser, toner, serum, skin gel, skin lotion, skin cream, face mask, sunscreen, conditioner, and hand sanitizer, but the selection of daily chemical products is not limited to these. Skin gels, skin lotions, and skin creams can be used on the face, and / or neck, and / or hands, and / or feet, and / or other parts of the body.

[0029] This application also provides a daily chemical product, including:

[0030] The irritating ingredient, at a level that can cause skin irritation in the test subject;

[0031] Anti-irritant dose of palm root extract.

[0032] When a daily chemical product contains irritating ingredients and the amount of these ingredients can cause skin irritation to the test subjects, adding an anti-irritant amount of Panax notoginseng root extract can effectively inhibit the irritation of the skin by the irritating ingredients. For example, when a daily chemical product contains SLS and the amount of this ingredient can cause skin irritation to the test subjects, adding an anti-irritant amount of Panax notoginseng root extract can effectively inhibit the irritation of the skin by SLS.

[0033] In one embodiment, the extract of *Gynostemma pentaphyllum* root is present in the daily chemical product at a mass fraction of 0.009% to 11%, such as 0.01%, 0.05%, 0.1%, 0.5%, 1%, 3%, 5%, 7%, 9%, 10%, etc., which can effectively inhibit the irritating effect of irritating ingredients on the skin.

[0034] In one embodiment, the irritating ingredient includes sodium lauryl sulfate (SLS), which is present in the daily chemical product at a mass fraction of 0.09% to 0.21%, such as 0.1%, 0.15%, or 0.2%. When the daily chemical product contains 0.09 wt% to 0.21 wt% SLS, it can cause skin irritation, while adding 0.009% to 11% *Gynostemma pentaphyllum* root extract can inhibit the skin irritation caused by SLS. When the daily chemical product contains 0.1 wt% SLS, adding 0.05 wt% or 0.5 wt% to 0.6 wt% *Gynostemma pentaphyllum* root extract can better inhibit the skin irritation caused by SLS.

[0035] The daily chemical products can be aqueous solutions, lotions, creams, or other dosage forms. In one embodiment, the daily chemical products include at least one of toothpaste, mouthwash, soap, shampoo, shower gel, dishwashing liquid, laundry detergent, laundry liquid, facial cleanser, toner, serum, skin gel, skin lotion, skin cream, face mask, sunscreen, conditioner, and hand sanitizer, but the selection of daily chemical products is not limited to these.

[0036] Compared with the prior art, the beneficial effects of this application are as follows: This application utilizes the root extract of Panax notoginseng as an anti-irritant in daily chemical products, which can prevent and / or treat skin irritation caused by irritants. It not only has good application prospects, but also expands the application of Panax notoginseng root extract. Attached Figure Description

[0037] Figure 1 These are photographs of the tissue morphology test in Example 2;

[0038] Figure 2 The statistical results of the number of cases of skin irritation reaction in different concentrations of Panax ginseng root extract groups in the SLS irritant patch test;

[0039] Figure 3The statistical results of the total skin irritation response scores of different concentrations of Panax ginseng root extract groups in the SLS irritant patch test;

[0040] Figure 4 The statistical results of the number of cases of skin irritation reaction in the 0.01wt% palm ginseng root extract group in the SLS irritant patch test;

[0041] Figure 5 The total score of skin irritation response in the 0.01wt% palm ginseng root extract group in the SLS irritant patch test is statistically analyzed.

[0042] Figure 6 Photographs of the process of treating skin irritation induced by nicotinic acid methyl ester with extracts of Panax ginseng root;

[0043] Figure 7 A photograph showing the process of treating hot water-induced skin irritation with ginseng root extract. Detailed Implementation

[0044] To better illustrate the purpose, technical solution, and advantages of this application, the following description, in conjunction with specific embodiments and comparative examples, aims to provide a detailed understanding of the content of this application, rather than limiting it. All other embodiments obtained by those skilled in the art without inventive effort are within the protection scope of this application. Unless otherwise specified, the experimental reagents and instruments involved in the implementation of this application are all commonly used reagents and instruments.

[0045] Example 1

[0046] This embodiment provides a palm-shaped ginseng root extract, the preparation method of which includes the following steps:

[0047] (1) Washing:

[0048] Take 10kg of Gynostemma pentaphyllum tuber and place it in a 1000L extraction tank. Add pure water to submerge the Gynostemma pentaphyllum tuber and stir slowly (10rpm) for 5 minutes. Stop stirring, discard the washing water, and repeat the washing process as described above until the washing water is basically clear. Stop washing to obtain clean Gynostemma pentaphyllum tuber.

[0049] (2) Infiltration:

[0050] Add pure water to the cleaned ginseng tuber at a ratio of ginseng tuber:pure water = 1kg:40L, start stirring, heat to 40℃, stop stirring, keep warm and soak overnight to fully moisten the ginseng tuber, take a sample and observe until there is no white core, and you will get a qualified moistened ginseng tuber.

[0051] (3) Grinding:

[0052] The above-mentioned soaked and qualified ginseng tuber was pulverized using a traditional Chinese medicine wet pulverizer to obtain a dispersion slurry. The particle size of the solid particles in the dispersion slurry was 60 mesh.

[0053] (4) Extraction:

[0054] Add pure water to the above dispersion slurry to dilute it and obtain a diluted dispersion. Ensure that the total water volume (L) in the diluted dispersion is 40 times the weight (kg) of the root of the Chinese ginseng, that is, the total water volume in the diluted dispersion is 400L. Then heat the diluted dispersion to 40°C and add neutral protease at 0.01% of the weight of the Chinese ginseng root. Homogenize and extract by circulating the mixture for 1 hour using a homogenizing pump to obtain an extract. The particle size of the solid particles in the extract is 100 mesh.

[0055] (5) Enzyme inactivation:

[0056] After extraction, heat the extract to 75°C and keep it at that temperature for 30 minutes to inactivate the enzymes.

[0057] (6) Coarse filtration:

[0058] The extract was filtered under pressure using a 400-mesh filter cloth and a medium-speed filter paper until the filtrate was basically clear, thus obtaining a coarse filtrate.

[0059] (7) Fine filtration:

[0060] The coarse filtrate is passed through a 0.45μm filter cartridge to obtain a fine filtrate, which is a clear and transparent solution.

[0061] (8) Concentration:

[0062] The above filtrate was concentrated under reduced pressure to 100L, and the concentration was stopped to obtain the concentrated solution.

[0063] (9) Drying;

[0064] The above-mentioned concentrate was placed in a hot air circulating oven (or vacuum drying oven, freeze dryer, or conveyor-type continuous vacuum low-temperature dryer) for drying until constant weight was obtained, resulting in a light yellow solid, which is the extract of Gynostemma pentaphyllum root.

[0065] The components of the palm root extract obtained in Example 1 were analyzed, and the results are shown in Table 1.

[0066] Table 1

[0067]

[0068]

[0069]

[0070] The morphology, pH, loss on drying, water solubility, total bacterial count, and total mold and yeast count of the extract obtained in Example 1 were tested. The test methods and results are shown in Table 2.

[0071] Table 2

[0072]

[0073]

[0074] Example 2

[0075] The inhibitory effect of the palm root extract obtained in Example 1 on SLS irritation was studied using a model.

[0076] The model construction mechanism is as follows: SLS is an anionic surfactant with amphiphilic (hydrophilic and lipophilic) properties. It is widely used as a foaming agent in detergents such as toothpaste, soap, shampoo, shower gel, dishwashing liquid, laundry powder, and laundry detergent. Approximately 95% of personal care products and household cleaning products contain SLS. As a chemical substance, SLS has a certain degree of skin irritation and is often used as a positive control for skin irritation reactions. It is also widely used in cosmetic safety assessments and screening for irritating ingredients in occupational skin diseases. When high concentrations of SLS come into contact with the skin, they can damage the lipid components in the skin barrier, leading to cell membrane damage, which in turn weakens the skin barrier and reduces the thickness of the living cell layer. FLG (Filaggrin) is a protein formed from the differentiation of keratinocytes at the terminal lamina. Its precursor, profilaggrin, is present in the granular layer. During the process of cells moving from the granular layer to the stratum corneum, profilaggrin is rapidly dephosphorylated by Caspase-14 (CASP14, cysteine ​​aspartate enzyme-14) to become filaggrin, which is then hydrolyzed into the main components of natural moisturizing factor (NMF) (PCA / UCA), thus playing an important role in barrier function and moisturizing function.

[0077] This embodiment utilizes SLS stimulation of a 3D skin model. A simulated human patch experiment was conducted to construct the "SLS-Epikutis" skin irritation injury model. Based on keratinocytes, a safe concentration of the sample was screened. Using a surface application method, simulating the human application process, the safe concentration of the sample was uniformly applied to the surface of the "Epikutis" skin model. The efficacy of the extract from *Gynostemma pentaphyllum* root in anti-irritation was assessed by detecting changes in tissue viability, morphological structure, and the content of the barrier protein FLG. Specific experiments are as follows:

[0078] The model was transferred to a 6-well plate (with 0.9 mL of model culture medium added beforehand). 25 μL of the prepared working solutions for each group were then added to the model surface. The plate was incubated in a CO2 incubator (37℃, 5% (v / v) CO2, 95% RH) for 24 h. After incubation, the model was cleaned with sterile PBS solution and sterile cotton swabs. These solutions were used for subsequent detection of tissue viability, tissue morphology, and FLG content. The working solution used for the blank control group (BC group) was PBS solution; the working solution used for the negative control group (NC group) was 0.2 wt% SLS solution (PBS solution as solvent); and the working solution used for the positive control group (PC group) was WY14643 working solution (prepared by dissolving 10 mg of WY14643 powder in 1 mL of PBS solution). In DMSO, a 30 mL MWY14643 stock solution was prepared. Then, 10 μL of this stock solution was added to 6 mL of model culture medium, and SLS was added to prepare a working solution containing 50 μL MWY14643 and 0.2 wt% SLS. The sample group (denoted as HR-10%) was a working solution containing 10 wt% Panax notoginseng root extract and 0.2 wt% SLS (solvent was PBS solution).

[0079] Tissue viability assay: 300 μL of 1 mg / mL MTT working solution was added to each well of a 24-well plate. After incubation, the model was transferred to the 24-well plate, and the plate was incubated in a constant temperature incubator (37℃, 5% CO2, 95% RH) for 3 hours. After MTT incubation, the model was transferred to a new 24-well plate, 2 mL of isopropanol was added, and the plate was incubated overnight at 4℃ to dissolve the model. The isopropanol extract was then allowed to flow from the model into the 24-well plate. Two 200 μL aliquots of the isopropanol extract were taken from each well and added to the corresponding wells of a 96-well plate, and labeled. Isopropanol was used as a blank control for absorbance assay. The absorbance was read at 570 nm using a microplate reader to detect tissue viability. Statistical analysis was performed using T-Test, and the results are shown in Table 3.

[0080] Detection of tissue morphological changes: The model used for tissue morphology was fixed with 4 wt% paraformaldehyde for 24 hours. After fixation, the model was circumcised, stained with H&E, and photographed under a microscope to observe the tissue morphology. Results are shown below. Figure 1 .

[0081] Detection of FLG (Fragrance Inhibitory Glucose) content: Paraffin sections were baked for 4 hours, then successively immersed in xylene, anhydrous ethanol, 95% (v / v) ethanol, and 75% (v / v) ethanol. The paraffin sections were then autoclaved in 0.01M sodium citrate antigen retrieval solution. Afterwards, each section was incubated with 1 drop of 3wt% H2O2 at room temperature, followed by blocking with serum homologous to the secondary antibody at 37°C, overnight incubation with primary antibody working solution at 4°C, incubation with secondary antibody working solution at room temperature, and incubation with ABC complex solution at room temperature. Then, 1 drop of DAB solution was added for microscopic observation, followed by hematoxylin counterstaining, dehydration with a gradient of alcohols (75%, 95%, 100%, 100%, all volume fractions), immersion in xylene, and finally mounting with neutral resin for observation to complete immunohistochemical detection. Results are shown in Table 4.

[0082] Table 3

[0083] Sample group name Average tissue vitality (%) Standard deviation p-value Group BC 100.00 4.77 / NC Group 77.61 2.55 0.002## PC Group 82.29 1.2 0.045* HR-10% group 96.83 3.73 0.002**

[0084] Note: The significance of the negative control group compared with the blank control group is indicated by #, and the P value < 0.01 is indicated by ##; the significance of the positive control group and the sample group compared with the negative control group is indicated by *, and the P value < 0.05 is indicated by *, and the P value < 0.01 is indicated by **. The same applies to Table 4.

[0085] Table 4

[0086]

[0087]

[0088] As shown in Table 3, compared with the BC (blank control) group, the tissue viability of the NC (negative control) group was significantly reduced, indicating that the stimulation conditions in this experiment were effective.

[0089] Compared with the NC (negative control) group, the PC (WY14643) (positive control) group showed significantly improved tissue viability, indicating that the detection system was effective.

[0090] Compared with the NC (negative control) group, the HR-10% group showed a significant improvement in tissue vitality, indicating that the root extract of Panax notoginseng at a concentration of 10wt% can effectively reduce the irritation of SLS.

[0091] Depend on Figure 1 It can be seen that, compared with the BC (blank control) group, the NC (negative control) group showed that the stratum corneum of the epidermal model was loose and thickened, the living cell layer was damaged and vacuoles appeared, indicating that the SLS stimulation condition was effective.

[0092] Compared with the NC (negative control) group, the PC (WY14643) (positive control) group showed clear boundaries in the four-layer structure, compact cell arrangement in the live cell layer, and significant improvement in the loose and thickened stratum corneum, indicating that the detection system was effective.

[0093] Compared with the NC (negative control) group, the HR-10% group showed clearer boundaries in the four-layer structure, significantly improved damage to the living cell layer, and a significant reduction in the loosening and thickening of the stratum corneum. This indicates that the extract of Panax ginseng root at a concentration of 10wt% can effectively reduce the irritation of SLS.

[0094] As shown in Table 4, the FLG protein content in the negative control group was significantly lower than that in the blank control group, indicating that the stimulation conditions in this experiment were effective.

[0095] Compared with the negative control group, the FLG protein content in the positive control group was significantly increased, indicating that the detection system was effective.

[0096] Compared with the NC (negative control) group, the FLG protein content in the HR-10% group was significantly increased, indicating that the root extract of Panax notoginseng can reduce the damage of SLS to the skin barrier.

[0097] Example 3

[0098] The therapeutic effect of the extract of *Gynostemma pentaphyllum* root obtained in Example 1 on SLS irritation was studied using a human survey method. The specific test method is as follows:

[0099] Subject profile: 30 subjects, with a mean age of 48.33 ± 6.68 years, including 1 male and 29 females. All subjects had smooth back skin without significant large-area pigmentation or folliculitis, and had not participated in other back tests or undergone treatments such as scraping or cupping within the past month. None had used antihistamines within the past week or immunosuppressants within the past month; none had applied any anti-inflammatory drugs to the test site within the past two months; none had clinically unresolved inflammatory skin diseases; none had insulin-dependent diabetes mellitus; none were currently receiving treatment for asthma or other chronic respiratory diseases; none had received chemotherapy for cancer within the past 6 months; none had immunodeficiency or autoimmune diseases; none were breastfeeding or pregnant; none had undergone bilateral mastectomy or bilateral axillary lymph node dissection; none had scars, pigmentation, atrophy, port-wine stains, or other blemishes on the skin test site that affected the interpretation of test results; none had participated in other clinical trials; and none had a highly sensitive constitution.

[0100] Test Procedure: The SLS irritation patch test method was adopted, specifically as follows: The method for occlusive patch testing in the 2015 "Cosmetic Safety Technical Specifications" was followed. Qualified patch testing equipment (Beijing Baiyiyida Technology Development Co., Ltd., 20220601) was selected. Using the occlusive patch test method, each test substance (0.1 wt% SLS aqueous solution without *Gynostemma pentaphyllum* extract, 0.1 wt% SLS aqueous solution containing 1 wt% *Gynostemma pentaphyllum* extract obtained in Example 1, 0.1 wt% SLS aqueous solution containing 0.6 wt% *Gynostemma pentaphyllum* extract obtained in Example 1, and 0.1 wt% SLS aqueous solution containing 0.5 wt% *Gynostemma pentaphyllum* extract obtained in Example 1) was applied. Approximately 0.025 mL of the following solutions were placed in different spot testers: an aqueous solution containing 0.3 wt% of the extract of *Gynostemma pentaphyllum* obtained in Example 1, an aqueous solution containing 0.2 wt% of the extract of *Gynostemma pentaphyllum* obtained in Example 1, an aqueous solution containing 0.1 wt% of the extract of *Gynostemma pentaphyllum* obtained in Example 1, and an aqueous solution containing 0.05 wt% of the extract of *Gynostemma pentaphyllum* obtained in Example 1. These solutions were then randomly applied to eight different locations on the back of the subject using hypoallergenic adhesive tape. The test products were removed after 24 hours. Skin reactions were observed at 0.5, 24, and 48 hours after removal, and the scores of the skin reactions were recorded and calculated.

[0101] Skin reaction scoring criteria: Referencing the grading criteria for skin reactions in the 2015 "Cosmetic Safety Technical Specifications" for closed patch tests, but there are still differences in severity within the Grade 1 suspected reaction. Therefore, this grade is further subdivided into 4 levels, and marked with 0.25, 0.5, 0.75 and 1 point respectively, as shown in Table 5.

[0102] Table 5

[0103]

[0104] See results Figures 2-3(Each bar chart, from left to right, corresponds to the following solutions: 0.1 wt% SLS aqueous solution without *Gynostemma pentaphyllum* extract; 0.1 wt% SLS aqueous solution containing 1 wt% *Gynostemma pentaphyllum* extract obtained in Example 1; 0.1 wt% SLS aqueous solution containing 0.6 wt% *Gynostemma pentaphyllum* extract obtained in Example 1; 0.1 wt% SLS aqueous solution containing 0.5 wt% *Gynostemma pentaphyllum* extract obtained in Example 1; 0.1 wt% SLS aqueous solution containing 0.3 wt% *Gynostemma pentaphyllum* extract obtained in Example 1; and 0.1 wt% SLS aqueous solution containing 0.2 wt% *Gynostemma pentaphyllum* extract obtained in Example 1.) SLS aqueous solution, 0.1 wt% SLS aqueous solution containing 0.1 wt% of the extract of *Gynostemma pentaphyllum* obtained in Example 1, and 0.1 wt% SLS aqueous solution containing 0.05 wt% of the extract of *Gynostemma pentaphyllum* obtained in Example 1; compared with the group containing *Gynostemma pentaphyllum* extract, P value < 0.1 is indicated as #, P value < 0.05 is indicated as *, P value < 0.01 is indicated as ** (# in the bar chart of Figure 348H should be *, this is a typo by the reporter). It can be seen that using 0.05 wt% to 1 wt% *Gynostemma pentaphyllum* root extract can effectively reduce the irritation of SLS.

[0105] In addition, the above method was used to study the inhibitory effect of 0.01 wt% *Gynostemma pentaphyllum* root extract on SLS irritation. The study included 32 subjects with a mean age of 46.38 ± 8.80 years (2 males and 30 females). The test results are shown below. Figures 4-5 (Each bar chart, from left to right, corresponds to a 0.1 wt% SLS aqueous solution without *Gynostemma pentaphyllum* extract and a 0.1 wt% SLS aqueous solution containing 0.01 wt% *Gynostemma pentaphyllum* extract obtained in Example 1; compared with the group containing *Gynostemma pentaphyllum* extract, a P value < 0.1 is indicated as #, and a P value < 0.05 is indicated as *). It can be seen that using 0.01 wt% *Gynostemma pentaphyllum* root extract can effectively reduce the irritation of SLS.

[0106] Example 4

[0107] The effect of the palm root extract obtained in Example 1 on treating skin irritation was studied using a human survey method.

[0108] Preparation of Ginseng root extract samples: Weigh 0.25g and 0.125g of Ginseng root extract powder from Example 1, respectively, and add them to deionized water, with a total weight of 50g; stir gently in a 60℃ water bath and incubate overnight at 4℃ to prepare aqueous solutions of Ginseng root extract with concentrations of 0.5wt% and 0.25wt%.

[0109] Mechanism of action of methyl nicotinic acid (MN) irritation model: MN is found in guava, papaya, strawberry, beer, brandy, coffee, hazelnut, peanut, custard apple, and bourbon vanilla, and is a natural equivalent flavoring agent. In cosmetics and skincare products, MN primarily functions as a skin reddening agent, commonly used in lip products. The vasodilatory effect of MN in the skin is mainly mediated through the prostaglandin pathway.

[0110] Subject profile: 31 subjects, with a mean age of 36.36 ± 6.28 years, including 2 males and 29 females. The inner forearm showed no obvious hair loss, lesions, scars, or pigmentation. The test area was free of severe acne, eczema, allergic dermatitis, or other conditions that could significantly affect the observation of results, such as excessive scarring or hair growth. Participants did not participate in other clinical studies during the trial, were not pregnant or breastfeeding, or planned to conceive within six months of the study's conclusion, and were not long-term users of oral contraceptives. None of the subjects had serious systemic diseases (history of thyroid, liver, or cardiovascular diseases, dyslipidemia, anemia, coagulation disorders, infectious diseases, psoriasis, diabetes, etc.).

[0111] Experimental controls: negative control group (only stimulation, no product applied) and experimental group (sample applied after stimulation).

[0112] Experimental sites: Three 2.5cm*4cm areas were selected in the central region of the inner forearm (two areas were used for the experimental group, corresponding to 0.5wt% and 0.25wt% aqueous solutions of Gynostemma pentaphyllum root extract, respectively; one area was used for the control group), and the experimental group and control group were randomly assigned.

[0113] Test Procedure

[0114] 1) Clean your arm and sit quietly in a constant temperature and humidity laboratory for 30 minutes before performing baseline value testing. Test parameters: VISIA photo, skin redness, skin hemoglobin, recorded as "BL" test.

[0115] 2) To establish a methyl nicotinic acid (MN) stimulation model, pre-cut cotton pads were soaked in a prepared MN aqueous solution for 30 seconds, then applied to a pre-marked area for 1 minute. Afterward, the pads were removed, gently wiped clean with a dry tissue, and tested after 15 minutes (referred to as the "immediate" test). A sample of *Gynostemma pentaphyllum* root extract was then applied. Tests were conducted at 5, 15, 30, and 60 minutes after application of the extract, and scores were given according to Table 5. The results are shown in Table 5. Figure 6 ;

[0116] 3) After waiting 3 hours, establish a hot water stimulation model on other forearm areas of the subject, that is, completely immerse the test area of ​​the arm in a constant temperature hot water bath for 3 minutes, then remove it, gently wipe it clean with a paper towel, wait about 5 minutes, and then test. The results are shown in […]. Figure 7 .

[0117] Depend on Figures 6-7 It is known that the root extract of Panax notoginseng can effectively treat skin irritation induced by methyl nicotinic acid and hot water.

[0118] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of this application and are not intended to limit the scope of protection of this application. Although this application has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of this application without departing from the substance and scope of the technical solutions of this application.

Claims

1. Use of a Gastrodia elata root extract as an anti-irritant in the manufacture of a domestic chemical product, characterized in that, The palm root extract can prevent and / or inhibit the stimulation of the skin by a stimulation source in a daily chemical product; the mass fraction of the palm root extract in the daily chemical product is 0.009% to 11%; the stimulation source is at least one of sodium lauryl sulfate, methyl nicotinate, and hot water; The preparation method of the palm root extract comprises the following steps: cleaning the palm root with water; mixing the cleaned palm root with pure water, and heating and soaking until there is no white core; pulverizing the soaked palm root to obtain a dispersed slurry; adding pure water to the dispersed slurry for dilution, and then adding neutral protease for cyclic homogenization extraction to obtain an extracted solution; heating the extracted solution to inactivate the enzyme; coarsely filtering and finely filtering the extracted solution after enzyme inactivation to obtain a clear and transparent solution; concentrating the obtained clear and transparent solution to obtain a concentrated solution; drying the concentrated solution to obtain the palm root extract.

2. Use according to claim 1, wherein The mass fraction of the palm root extract in the daily chemical product is 0.05% or 0.5% to 0.6%.

3. The use according to claim 1, wherein The daily chemical product comprises at least one of toothpaste, mouthwash, soap, shampoo, shower gel, dishwashing detergent, laundry powder, laundry liquid, facial cleanser, toner, serum, skin jelly, skin emulsion, skin cream, facial mask, sunscreen, hair conditioner, and hand sanitizer.

4. A household chemical product characterized by It comprises: a stimulating component, the content of which can cause skin stimulation to a subject; an anti-stimulation amount of palm root extract; The mass fraction of the palm root extract in the daily chemical product is 0.009% to 11%; The stimulating component comprises sodium lauryl sulfate, and the mass fraction of the sodium lauryl sulfate in the daily chemical product is 0.09% to 0.21%; The preparation method of the palm root extract comprises the following steps: cleaning the palm root with water; mixing the cleaned palm root with pure water, and heating and soaking until there is no white core; pulverizing the soaked palm root to obtain a dispersed slurry; adding pure water to the dispersed slurry for dilution, and then adding neutral protease for cyclic homogenization extraction to obtain an extracted solution; heating the extracted solution to inactivate the enzyme; coarsely filtering and finely filtering the extracted solution after enzyme inactivation to obtain a clear and transparent solution; concentrating the obtained clear and transparent solution to obtain a concentrated solution; drying the concentrated solution to obtain the palm root extract.

5. The household chemical product according to claim 4, wherein The mass fraction of the palm root extract in the daily chemical product is 0.05% or 0.5% to 0.6%; and the mass fraction of the sodium lauryl sulfate in the daily chemical product is 0.1%.

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