A kind of experimental rabbit compound feed and application thereof

Abstract

The present invention relates to a feed additive, comprising a lime extract, a roxburgh roxburgh extract, and a polygonatum polysaccharide. The feed additive may further comprise radish seeds and Shenqu. The weight ratio of the lime extract, the roxburgh roxburgh extract, the polygonatum polysaccharide, the radish seeds, and the Shenqu is preferably 0.5-4:0.5-2:0.5-2:0.5-4:0.5-2. The present invention also relates to a compound feed for experimental rabbits, comprising 5-30 parts of corn, 7-35 parts of soybean hulls, 20-50 parts of alfalfa, 5-20 parts of soybean meal, 3-18 parts of wheat, 1-15 parts of bran, 0.1-1.5 parts of stone powder, 0.3-1.2 parts of salad oil, 0.1-2 parts of brewer's yeast powder, 0.5-4 parts of calcium hydrogen phosphate, 0.1-1 parts of feed-grade sodium chloride, 1-5 parts of multivitamin and multi-mineral premix, and 0.1-4 parts of a feed additive. The experimental rabbit compound feed containing the feed additive of the present invention is nutritionally comprehensive and balanced, highly safe, and easily digestible and absorbable. The compound feed not only promotes the rabbit's appetite, but also promotes the rabbit's growth and development, maintains intestinal health, enhances the rabbit's anti-stress ability, and reduces the rabbit's fungal infection rate.

Description

Technical Field

[0001] The present invention relates to the technical field of experimental animal feed, in particular to an experimental rabbit compound feed and application thereof. Background Art

[0002] Rabbits, as experimental animals, are widely used in biology, medicine, and other fields, making irreplaceable contributions to scientific research. Rabbits are monogastric herbivores with a well-developed cecum, which allows them to digest crude fiber at a high rate. If rabbits are not supplied with enough fiber during their growth, this will affect the stability of the large intestinal flora, leading to indigestion and various diseases. Unlike domestic rabbits, experimental rabbits are carefully cared for in laboratories year-round, especially those kept in sterile and SPF environments, which require extremely stringent growth conditions. These rabbits often consume concentrated feed and rarely green feed during their growth. This, combined with the various stressful conditions of frequent scientific experiments, results in poor palatability, reduced feed intake, disrupted intestinal flora balance, cecal flatulence or diarrhea, and ultimately death.

[0003] At present, when rabbits have poor palatability, flavoring agents are generally added to improve the smell of feed. However, this cannot fundamentally improve the rabbit's body resistance, nor can it strengthen the rabbit's stomach and spleen and balance the disordered flora. Sometimes antibiotics are used to treat problems such as poor appetite, gastrointestinal bloating, and diarrhea, which often leads to drug residues. The metabolites of antibiotics are often excreted with feces, urine and other excretions, causing pollution to the animal room environment.

[0004] Therefore, it is necessary to develop a compound feed that can strengthen the stomach and spleen, balance the intestinal flora, improve immunity and meet the nutritional requirements of experimental rabbits for growth and development. Summary of the Invention

[0005] In order to overcome at least one problem existing in the prior art, the present invention provides a feed additive and an experimental rabbit compound feed containing the feed additive. The experimental rabbit compound feed is nutritionally comprehensive and balanced, highly safe, and easily digestible and absorbable. While protecting intestinal health, it can also effectively enhance the physical fitness and improve the rabbit's anti-stress ability.

[0006] To achieve the above object, the present invention adopts the following technical solutions:

[0007] The first aspect of the present invention is to provide a feed additive comprising at least one of a sour orange extract, a roxburghii roxburghii extract, and a polygonatum polysaccharide.

[0008] Furthermore, the feed additive is composed of lime extract, roxburgh roxburgh extract and polygonatum polysaccharide, and the weight ratio of the lime extract, roxburgh roxburgh extract and polygonatum polysaccharide is 0.5-4:0.5-2:0.5-2; preferably, the weight ratio of the lime extract, roxburgh roxburgh extract and polygonatum polysaccharide is 1-2:1:1; more preferably, the weight ratio of the lime extract, roxburgh roxburgh extract and polygonatum polysaccharide is 2:1:1.

[0009] Furthermore, the feed additive also includes radish seeds and Shenqu, and the weight ratio of the lime extract, roxburgh roxburgh extract, polygonatum polysaccharide, radish seeds, and Shenqu is 0.5-4:0.5-2:0.5-2:0.5-4:0.5-2; preferably, the weight ratio of the lime extract, roxburgh roxburgh extract, polygonatum polysaccharide, radish seeds, and Shenqu is 1-2:1:1:1-2:1; more preferably, the weight ratio of the lime extract, roxburgh roxburgh extract, polygonatum polysaccharide, radish seeds, and Shenqu is 2:1:1:2:1.

[0010] Among the above-mentioned feed additives, lime extract has the functions of regulating qi and relieving chest tightness, lifting the anus and relieving bloating, and acting as a stomachic. It is natural, safe, green and healthy, and can fundamentally promote animal appetite, invigorate the spleen and strengthen the stomach, inhibit harmful microorganisms, increase the number of beneficial bacteria, promote intestinal development, balance intestinal flora, and enhance the body's ability to resist stress.

[0011] Among the above-mentioned feed additives, sea buckthorn extract and polygonatum polysaccharide are more commonly used in humans, but are currently less used in animals, and their efficacy in animals has not yet been clarified. Sea buckthorn extract is rich in nutrients such as vitamin C and superoxide dismutase SOD, and has the ability to enhance antioxidant and immunity. Polygonatum polysaccharide has the effect of enhancing immunity and inhibiting the incidence of fungal diseases in rabbits, allowing them to maintain healthy skin and hair.

[0012] Among the aforementioned feed additives, radish seeds are pungent, sweet, and neutral in nature, entering the lung, spleen, and stomach meridians, promoting digestion and relieving bloating, lowering qi and resolving phlegm; while Shenqu (Shenqu) is sweet, pungent, and warm in nature, entering the spleen and stomach meridians, strengthening the spleen and harmonizing the stomach, and promoting digestion and eliminating stagnation. Both radish seeds and Shenqu can eliminate stagnation, dissipate bloating, and soothe the stomach and stimulate appetite.

[0013] In the above-mentioned feed additives, sour orange extract (or with radish seeds, Shenqu combination) can ensure that rabbits have a great appetite, promote the digestion of food in the stomach, and ensure the health of the upper end of the digestive tract; Polygonatum sibiricum polysaccharide and roxburghii roxburghii extract can ensure the balance of the flora at the rear end of the digestive tract, anti-inflammatory and bactericidal, and enhance the antioxidant capacity and immunity of the digestive tract. The present invention is equivalent to carrying out a "two-pronged approach" to the health of the digestive tract. While protecting intestinal health, it can also well enhance physical fitness, improve the anti-stress ability of rabbits, and reduce the infection rate of rabbit fungi. The collocation of each component in the above-mentioned feed additives can essentially enhance body resistance, increase the palatability of experimental rabbits, invigorate the spleen and stimulate appetite, and increase feed intake; Simultaneously promote intestinal peristalsis, increase beneficial bacteria in the intestine, reduce harmful bacteria, improve intestinal microecological balance, reduce the generation of diarrhea and flatulence in experimental rabbits, improve the immunity of experimental rabbits, and enhance the resistance of body to changes in external environment.

[0014] It is understandable that the components of the above-mentioned feed additives can be purchased commercially or prepared by conventional methods. For example, the sour orange extract is from Waters Bio, the sour orange extract is a brown-yellow powder, 100 mesh, and is water-soluble; the roxburghii extract is purchased from Jianpeptide Biotechnology Co., Ltd., and the SOD activity of the roxburghii extract is 10,000 U / G, VC5%; the polygonatum polysaccharide is purchased from Shanxi Senwan Zhuoyuan Biotechnology Co., Ltd., product number: polygonatum polysaccharide 99%, brown-yellow powder, 100 mesh.

[0015] A second aspect of the present invention provides a compound feed for experimental rabbits, comprising any of the feed additives described in the first aspect of the present invention and a base feed; wherein the weight of the feed additive accounts for 0.1 to 4% based on 100% of the mass of the compound feed. If the feed additive is added in an amount exceeding 4%, harmful intestinal bacteria in the experimental rabbits cannot be effectively suppressed, the effect on their stress resistance is insignificant, and feed costs increase. Concentrations below 0.1% are ineffective in improving the intestinal health and immune system of the experimental rabbits.

[0016] Furthermore, the basic feed includes, by weight: 5-30 parts of corn, 7-35 parts of soybean hulls, 20-50 parts of alfalfa, 5-20 parts of soybean meal, 3-18 parts of wheat, 1-15 parts of bran, 0.1-1.5 parts of stone powder, 0.3-1.2 parts of salad oil, 0.1-2 parts of brewer's yeast powder, 0.5-4 parts of calcium hydrogen phosphate, 0.1-1 parts of feed-grade sodium chloride, and 1-5 parts of multi-vitamin and multi-mineral premix.

[0017] Furthermore, in parts by weight, the experimental rabbit compound feed includes: 0.1-2 parts of feed additives, 10-20 parts of corn, 10-30 parts of soybean hulls, 30-40 parts of alfalfa, 10-15 parts of soybean meal, 5-15 parts of wheat, 1-10 parts of bran, 0.1-0.9 parts of stone powder, 0.5-1 parts of salad oil, 0.1-0.5 parts of brewer's yeast powder, 0.5-2 parts of calcium hydrogen phosphate, 0.1-0.5 parts of feed-grade sodium chloride, and 3-4 parts of multivitamin and multi-mineral premix; wherein the feed additives are composed of lime extract, sea buckthorn extract, polygonatum polysaccharide, radish seed, and Shenqu, and the weight ratio thereof is 1-2:1:1:1-2:1.

[0018] Furthermore, in parts by weight, the experimental rabbit compound feed includes: 1 part of feed additives, 20 parts of corn, 10 parts of soybean hulls, 32 parts of alfalfa, 11 parts of soybean meal, 12 parts of wheat, 7 parts of bran, 0.6 parts of stone powder, 0.5 parts of soybean oil, 0.5 parts of brewer's yeast powder, 1 part of calcium hydrogen phosphate, 0.4 parts of feed-grade sodium chloride, and 4 parts of multivitamin and multi-mineral premix; wherein the feed additives are composed of lime extract, sea buckthorn extract, and polygonatum polysaccharide, and the weight ratio thereof is 1 to 2:1:1.

[0019] Furthermore, each kilogram of multivitamin premix includes vitamin A 150000-300000 IU, vitamin D 1750-35000 IU, vitamin E 1250-2500 IU, vitamin K 7.5-15 mg, vitamin B1 175-350 mg, vitamin B2 200-400 mg, vitamin B6 150-300 mg, vitamin B 12 0.5-1mg, niacin 1000-2000mg, pantothenic acid 300-600mg, folic acid 25-50mg, biotin 5-500mg, choline 25000-50000mg, iron 2500-5000mg, copper 225-450mg, manganese 1000-2000mg, zinc 1250-2500mg. Preferably, per kilogram of multivitamin premix includes vitamin A 200000IU, vitamin D1750-35000IU, vitamin E 2000IU, vitamin K 10mg, vitamin B1 250mg, vitamin B2 300mg, vitamin B6 200mg, vitamin B 12 1mg, niacin 1500mg, pantothenic acid 500mg, folic acid 40mg, biotin 50mg, choline 30000mg, iron 3000mg, copper 350mg, manganese 1500mg, zinc 1500mg.

[0020] The third aspect of the present invention is to provide a method for preparing the experimental rabbit compound feed described in any one of the second aspects of the present invention, comprising the steps of:

[0021] S1. Grinding a predetermined amount of corn, wheat, soybean hulls, soybean meal, and alfalfa to obtain a first powder;

[0022] S2, mixing a predetermined amount of bran, brewer's yeast powder and the first powder obtained in step S1 once, and grinding them twice to obtain a second powder;

[0023] S3, a predetermined amount of lime extract, roxburghii extract and polygonatum polysaccharide (powdered radish seeds and Shenqu can be additionally added), soybean oil, stone powder, calcium hydrogen phosphate, feed-grade sodium chloride, multivitamin and multi-mineral premix are mixed with the second powder obtained in step S2 for a second time to obtain a mixture;

[0024] S4, granulating, drying, and cooling the mixture obtained in step S3 to obtain the experimental rabbit compound feed;

[0025] Furthermore, in the preparation method, a 1.5 mm sieve is used for both the primary and secondary crushing; and / or the granulation temperature is 75-80°C, and the obtained particles have a diameter of 6 mm; and / or the drying operating conditions are: upper layer temperature 55°C, lower layer temperature 90°C, and drying time 15 minutes.

[0026] The fourth aspect of the present invention is to provide an application of the feed additive described in any one of the first aspects of the present invention, or the experimental rabbit compound feed described in any one of the second aspects of the present invention, which is selected from at least one of the following applications: application in promoting the growth and development of experimental rabbits, application in maintaining the intestinal health of experimental rabbits, application in enhancing the anti-stress ability of experimental rabbits, and application in preventing or treating skin diseases of experimental rabbits.

[0027] Furthermore, the skin diseases of the experimental rabbits include skin diseases caused by fungal infections.

[0028] Furthermore, the main pathogens of the skin diseases include Epidermophyton, Trichophyton, Microsporum, Candida albicans, Trichophyton rubrum, Trichophyton violaceum, Trichophyton schrenckii, Microsporum spp., etc., and specifically can be Trichophyton rubrum.

[0029] Furthermore, the breeds of the experimental rabbits include New Zealand white rabbits, Japanese big-eared white rabbits, blue-purple rabbits and Chinese white rabbits.

[0030] Compared with the prior art, the present invention adopts the above technical solution to achieve the following beneficial effects:

[0031] The components of the feed additive of the present invention are used in combination to have a synergistic effect. Sour orange extract (or its combination with radish seeds and Shenqu) is a stomachic agent that can enhance the appetite of animals, eliminate intestinal flatulence, and simultaneously reduce harmful intestinal flora, reduce intestinal inflammatory factors, and protect intestinal health; Rosa roxburghii extract is rich in vitamin C, which is a nutrient necessary for the normal functioning of the animal immune system. Supplementation of vitamin C can inhibit the release of corticosterone, enhance stress resistance, and have a synergistic effect on the immunomodulation of polygonatum polysaccharide; polygonatum polysaccharide can inhibit the incidence of fungal diseases in rabbits, so that they maintain healthy skin and hair. The above several substances are added to the above feed, and the sour orange extract is equivalent to a substrate. After the harmful intestinal flora barrier is cleared, the rosa roxburghii extract is equivalent to a synergist, which performs intestinal stabilization, making it easier for polygonatum polysaccharide to better play a role.

[0032] The experimental rabbit compound feed of the present invention contains the above-mentioned feed additives and excellent basic feed, and is nutritionally comprehensive and balanced, highly safe, and easily digestible and absorbable. The above-mentioned compound feed not only promotes the rabbit's appetite, but also promotes the rabbit's growth and development, maintains intestinal health, enhances the rabbit's anti-stress ability, and reduces the rabbit's fungal infection rate. DETAILED DESCRIPTION

[0033] The technical solutions in the embodiments of the present invention will be described clearly and completely below. Obviously, the embodiments described are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative work are within the scope of protection of the present invention. The experimental methods in the following examples where specific conditions are not specified are generally measured in accordance with national standards. The experimental materials in the following examples where the sources are not specified are all commercially available raw materials. The equipment used in each step of the following examples is conventional equipment. If there are no corresponding national standards, the steps are carried out in accordance with general international standards, conventional conditions, or conditions recommended by the manufacturer. Unless otherwise specified, all parts are by weight and all percentages are by mass percentages. Unless otherwise defined or specified, all professional and scientific terms used in the present invention have the same meanings as those familiar to those skilled in the art. In addition, any methods and materials similar or equivalent to those described herein can be applied to the methods of the present invention.

[0034] It should be noted that, in the absence of conflict, the embodiments and features of the embodiments of the present invention can be combined with each other. The present invention will be further described below with reference to specific embodiments, but this does not limit the present invention.

[0035] Example 1 - Feed Additive

[0036] This example provides a feed additive. Select lime extract, roxburghii extract, polygonatum polysaccharide, radish seed, and Shenqu (Chinese Herbal Medicine) according to the mass ratios in the table below and mix thoroughly. Lime extract was purchased from Waters Biotechnology as a brownish-yellow powder, 100 mesh, and water-soluble. Roxburghii extract was purchased from Jianpeptide Biotechnology Co., Ltd., with a SOD activity of 10,000 U / g and VC of 5%. Polygonatum polysaccharide was purchased from Shanxi Senwan Zhuoyuan Biotechnology Co., Ltd., under the product number "99% Polygonatum polysaccharide," a brownish-yellow powder, 100 mesh. Shenqu and radish seed ultrafine powders, 120 mesh, were prepared as powders with a particle size of 48 μm. Usage: Add the above feed additives directly to the base feed at a weight ratio of 0.1% to 4%. Studies have shown that a 1% addition yields the best results when combined with the base feed.

[0037]

[0038] Example 2 - Experimental rabbit compound feed and its preparation

[0039] This example provides an experimental rabbit compound feed, which includes the feed additives and basic feed in Example 1. The specific raw material composition is shown in the following table, wherein: the feed additives are group A additives (lime extract + roxburgh roxburgh extract + polygonatum polysaccharide) and group B additives (lime extract + roxburgh roxburgh extract + polygonatum polysaccharide + radish seed + Shenqu) in Example 1, and each kilogram of multivitamin and multimineral premix includes vitamin A 200000 IU, vitamin D 1750-35000 IU, vitamin E 2000 IU, vitamin K 10 mg, vitamin B1 250 mg, vitamin B2 300 mg, vitamin B6 200 mg, vitamin B 12 1mg, niacin 1500mg, pantothenic acid 500mg, folic acid 40mg, biotin 50mg, choline 30000mg, iron 3000mg, copper 350mg, manganese 1500mg, zinc 1500mg.

[0040]

[0041] The preparation method of the above feed composition comprises the following steps:

[0042] Step 1: Corn, wheat, soybean meal, soybean hulls, and alfalfa are automatically fed into the central control computer and crushed for the first time through a 1.5mm sieve to obtain powder.

[0043] Step 2: Automatically mix the bran, brewer's yeast powder and the powder from step 1, and then perform the first mixing for 3 minutes.

[0044] Step 3: The mixture obtained in step 2 is crushed for the second time, and the sieve is 1.5 mm.

[0045] Step 4: Add lime extract, roxburghii extract and polygonatum polysaccharide (if containing radish seeds and Shenqu, add them in this step), soybean oil, stone powder, calcium hydrogen phosphate, feed-grade sodium chloride, and multivitamin premix to the mixture obtained in step 3, and then perform a second mixing for 3 minutes.

[0046] Step 5: Granulate the raw materials mixed in step 4 at a granulation temperature of 75-80° C. to obtain granules with a diameter of 6 mm.

[0047] Step 6: The pellet feed obtained in step 5 is transferred to an oven for drying. The upper layer temperature of the oven is 55°C, the lower layer temperature is 90°C, and the drying time is 15 minutes.

[0048] Step 7: The feed dried in step 6 is transferred to a cooling bin for 10 minutes. The feed temperature is within the room temperature of 20-25°C, and the compound feed is thus prepared.

[0049] Step 8: Sterile-grade experimental rabbit feed requires the compound feed obtained in step 7 to be sterilized by high pressure or irradiation.

[0050] Example 3 - Application of experimental rabbit compound feed

[0051] 300 healthy weaned New Zealand rabbits weighing 600-800 g were selected, half male and half female. A single-factor completely randomized block experiment was used and divided into 6 groups, 10 replicates per group, 10 rabbits per replicate. The decibels were blank group, lime extract group, roxburghii extract group, polygonatum polysaccharide group, rabbit food group 1 (Example 2), and rabbit food group 2 (Example 2).

[0052] The blank group was a basic feed without feed additives, the sour orange extract group was a basic feed without sour orange extract in the feed additives of group A, the roxburgh roxburgh extract group was a basic feed without sour orange extract in the feed additives of group A, the polygonatum polysaccharide group was a basic feed without sour orange polysaccharide in the feed additives of group A, rabbit feed group 1 was a combination of sour orange extract + roxburgh roxburgh extract + polygonatum polysaccharide + basic feed, and rabbit feed group 2 was a combination of sour orange extract + roxburgh roxburgh extract + polygonatum polysaccharide + radish seed + Shenqu + basic feed.

[0053] All the above groups had the same feeding conditions and disease control measures, and the experimental period was 30 days.

[0054] (1) Record the feed intake, body weight, diarrhea rate and mortality of each group of experimental rabbits. The results are as follows:

[0055] Table 1 - Production performance of experimental rabbits during the trial

[0056] project Blank group Lime extract group Rosa roxburghii extract group Polygonatum sibiricum polysaccharide group 1 set of rabbit food Rabbit food 2 groups Initial body weight, g 800.3±45.1 801.5±43.4 793.1±39.4 795.6±31.4 799.1±35.7 800.3±32.4 Final weight, g <![CDATA[1756.6 b ±70.1]]> <![CDATA[1768.7 b ±50.5]]> <![CDATA[1760 b .5±45.8]]> <![CDATA[1754.6 b ±48.1]]> <![CDATA[1896.2 a ±48.3]]> <![CDATA[1905.1 a ±46.7]]> Average daily weight gain g / d <![CDATA[31.87 b ±35.0]]> <![CDATA[32.24 b ±26.7]]> <![CDATA[32.25 b ±30.7]]> <![CDATA[31.9 b ±40.5]]> <![CDATA[36.57 a ±35.1]]> <![CDATA[37.17 a ±35.3]]> Daily feed intake, g <![CDATA[100.0 b ±5.2]]> <![CDATA[105.0 b ±6.8]]> <![CDATA[108.4 b ±6.0]]> <![CDATA[104.2 b ±3.4]]> <![CDATA[1118.4 a ±6.1]]> <![CDATA[120.7 a ±5.1]]> Diarrhea rate% 10.5％ 8.2％ 7.4％ 8.5％ 1.0％ 0.9％ mortality rate% 5％ 3％ 3％ 2％ 0％ 0％

[0057] Note: a means there is a significant difference when p < 0.05, the same below.

[0058] From the experimental results in Table 1 above, it can be seen that feeding the lime extract group, the roxburghii extract group, the polygonatum polysaccharide group, the rabbit diet group 1, and the rabbit diet group 2 can alleviate the diarrhea and mortality of the experimental rabbits to a certain extent. The feed intake and weight gain of the experimental rabbits in the lime extract group, the roxburghii extract group, and the polygonatum polysaccharide group are not much different from those in the blank group, but the effects of the rabbit diet group 1 and the rabbit diet group 2 are comparable, and both can significantly achieve weight gain in the experimental rabbits, effectively alleviate diarrhea, and significantly reduce mortality. This shows that the effect of adding lime extract, roxburghii extract group, and polygonatum polysaccharide to the basic feed on the growth of experimental rabbits is significantly better than that of the feed group adding a single additive.

[0059] (2) On the second day after the feeding experiment, one rabbit was randomly selected from each replicate. One of the experimental rabbits was slaughtered and its liver was removed. The liver was ground and mixed with phosphate buffered saline (PBS) at a ratio of 1:9. Blood was collected from the heart and centrifuged at 4000 r / min for 10 min. The supernatant was aliquoted and stored in a -80°C refrigerator. Liver and serum superoxide dismutase (SOD) activity, total antioxidant capacity (T-AOC), and malondialdehyde (MDA) content were determined by biochemical methods. The results are shown in Table 2.

[0060] Table 2 - Antioxidant indexes of serum and liver of experimental rabbits during the experiment

[0061]

[0062] From the experimental results in Table 2 above, it can be seen that compared with the blank group, the orange extract group, the roxburghii extract group, and the polygonatum polysaccharide group had a certain effect on the superoxide dismutase and malondialdehyde contents, but the effect was not significant; rabbit diet group 1 and rabbit diet group 2 both significantly increased the hydrogen peroxide dismutase content and significantly reduced the malondialdehyde content, but the effects of the two were equivalent, which shows that the effect of adding orange extract, roxburghii extract group, and polygonatum polysaccharide to the basic feed at the same time in improving the antioxidant capacity is significantly better than the feed group adding a single additive.

[0063] (3) The levels of immunoglobulin M (IgM), immunoglobulin G (IgG), and immunoglobulin A (IgA) in serum were determined. The results are shown in Table 3.

[0064] Table 3 - Serum immune indicators of experimental rabbits during the experiment

[0065] project Blank group Lime extract group Rosa roxburghii extract group Polygonatum sibiricum polysaccharide group 1 set of rabbit food Rabbit food 2 groups Immunoglobulin A (g / L) <![CDATA[0.7±0.45 b ]]> <![CDATA[0.74±0.42 b ]]> <![CDATA[0.82±0.52 b ]]> <![CDATA[0.84±0.54 b ]]> <![CDATA[0.98±0.23 a ]]> <![CDATA[0.89±0.32 a ]]> Immunoglobulin G (g / L) <![CDATA[5.38±0.23 b ]]> <![CDATA[5.39±0.38 b ]]> <![CDATA[5.45±0.42 b ]]> <![CDATA[5.66±0.40 b ]]> <![CDATA[6.54±0.31 a ]]> <![CDATA[6.45±0.38 a ]]> Immunoglobulin M (g / L) <![CDATA[0.62±0.12 b ]]> <![CDATA[0.64±0.20 b ]]> <![CDATA[0.65±0.19 b ]]> <![CDATA[0.67±0.22 b ]]> <![CDATA[0.83±0.16 a ]]> <![CDATA[0.79±0.21 a ]]>

[0066] From the experimental results in Table 3 above, it can be seen that compared with the blank group, the orange extract group, the roxburghii extract group, and the polygonatum polysaccharide group had little effect on IgM, IgG, and IgA; rabbit diet group 1 and rabbit diet group 2 could significantly increase the levels of IgM, IgG, and IgA in the serum of the experimental rabbits, but the effect of rabbit diet group 2 was weaker than that of rabbit diet group 1. This shows that the effect of adding orange extract, roxburghii extract group, and polygonatum polysaccharide to the basic feed at the same time in improving the stress capacity of the experimental rabbits is significantly better than that of the feed group with a single additive, and is better than the feed with radish seeds and Shenqu added together.

[0067] (4) The microbial status of cecal chyme was detected. The results are shown in Table 4.

[0068] Table 4 - Microbial indicators of cecal chyme of experimental rabbits during the experiment

[0069] project Blank group Lime extract group Rosa roxburghii extract group Polygonatum sibiricum polysaccharide group 1 set of rabbit food Rabbit food 2 groups Total bacterial count, log CFU / g 10.53±0.42 9.64±0.56 10.56±0.48 10.59±0.48 10.99±0.45 10.68±0.36 Escherichia coli, log CFU / g <![CDATA[5.26±0.39 b ]]> <![CDATA[4.32±0.45 b ]]> <![CDATA[5.20±0.59 b ]]> <![CDATA[5.82±0.55 b ]]> <![CDATA[0.76±0.29 a ]]> <![CDATA[0.78±0.32 a ]]> Bifidobacterium, log CFU / g <![CDATA[1.98±0.55 b ]]> <![CDATA[2.71±0.49 b ]]> <![CDATA[2.58±0.18 b ]]> <![CDATA[2.71±0.44 b ]]> <![CDATA[4.58±0.16 a ]]> <![CDATA[4.49±0.21 a ]]> Lactobacillus, log CFU / g <![CDATA[2.23±0.09 b ]]> <![CDATA[2.56±0.03 b ]]> <![CDATA[3.98±0.06 a ]]> <![CDATA[2.56±0.03 b ]]> <![CDATA[5.98±0.05 a ]]> <![CDATA[5.67±0.04 a ]]>

[0070] From the experimental results in Table 4 above, it can be seen that: compared with the blank group, the orange extract group, the roxburghii extract group, and the polygonatum polysaccharide group can all increase the content of probiotics in the cecal chyme to a certain extent; the rabbit diet group 1 and the rabbit diet group 2 can both significantly increase the content of probiotics in the cecal chyme, and the effects are not much different. This shows that the effect of adding orange extract, roxburghii extract group, and polygonatum polysaccharide to the basic feed at the same time in improving the intestinal health of experimental rabbits is significantly better than that of the feed group adding a single additive.

[0071] (5) Recovery of experimental rabbits from skin fungal infection

[0072] Select 300 weaned New Zealand rabbits with a body weight of 600-800g, half male and half female. Adopt single factor completely randomized block experiment method, be divided into 6 groups, 10 repetitions in every group, often repeat 10 rabbits, be divided into blank group, sour orange extract group, roxburghii extract group, polygonatum polysaccharide group, rabbit grain 1 group (embodiment 2), rabbit grain 2 groups (embodiment 2). Randomly pick two in every group of rabbits, cut the fur of 2.5cm × 2.5cm area on both sides of back, clean, scratch gently (with slightly blood spot as advisable) with sterilized fine sandpaper, inoculate the rubrum obtained by separating from sick rabbit skin, carry out infection after attacking toxic substances for one week, carry out feeding of each group of feed respectively, observe the recovery situation of feeding each group of feed after one week, its result is as shown in Table 5.

[0073] Table 5 - Recovery of experimental rabbits from skin fungal infections during the experiment

[0074]

[0075] Recovery rate: refers to the percentage of rabbits with no new lesions on the coat, less or new coat regrowth, and itching that has not completely disappeared or has completely disappeared.

[0076] The experimental results in Table 5 above show that the recovery rate of the Polygonatum sibiricum polysaccharide group was significantly better than that of the blank group, the lime extract, and the Rosa roxburghii extract groups. However, the recovery rates of Rabbit Diet Group 1 and Rabbit Diet Group 2 both exceeded 90%, with Rabbit Diet Group 2 achieving 100% recovery. Polygonatum sibiricum polysaccharide has antifungal properties, while lime extract and Rosa roxburghii extract contain vitamin C and superoxide dismutase, which can eliminate free radicals, alleviate inflammation, and improve the skin's barrier function. Radish seed contains radisin, a natural anti-inflammatory ingredient, and Shenqu contains multiple anti-inflammatory ingredients that can effectively inhibit and reduce inflammatory responses. The combination of these components can assist Polygonatum sibiricum polysaccharide in exerting its therapeutic effect against fungal infections.

[0077] It can be seen from the above embodiments that the experimental rabbit compound feed involved in the present invention is nutritionally comprehensive and balanced, highly safe, and easy to digest and absorb; the combination of roxburghii extract, polygonatum polysaccharide and sour orange extract (or their combination with radish seeds and Shenqu) has a synergistic effect, specifically: (1) it can increase the palatability of experimental rabbits, wake up the spleen and appetite, and increase feed intake; it can enhance the appetite of animals, eliminate intestinal flatulence, and at the same time reduce harmful intestinal bacteria, reduce intestinal inflammatory factors, and protect intestinal health; (2) it promotes the growth and development of rabbits, enhances the body's antioxidant capacity, and improves the rabbit's anti-stress ability; and (3) reduces rabbit skin diseases caused by fungal infections.

[0078] While the specific embodiments of the present invention have been described in detail above, these are intended to be exemplary only, and the present invention is not limited thereto. For those skilled in the art, any equivalent modifications and substitutions to the present invention are also within the scope of the present invention. Therefore, any equivalent changes and modifications made without departing from the spirit and scope of the present invention are intended to be encompassed within the scope of the present invention.

Claims

1. A feed additive, characterized in that The feed additive consists of sour orange extract, roxburgh roxburgh extract and polygonatum polysaccharide, and the weight ratio of the sour orange extract, roxburgh roxburgh extract and polygonatum polysaccharide is 0.5-4:0.5-2:0.5-2.

2. The feed additive according to claim 1, characterized in that The feed additive consists of lime extract, roxburgh roxburgh extract, polygonatum polysaccharide, radish seeds and Shenqu, and the weight ratio of the lime extract, roxburgh roxburgh extract, polygonatum polysaccharide, radish seeds and Shenqu is 0.5-4:0.5-2:0.5-2:0.5-4:0.5-2.

3. A compound feed for experimental rabbits, characterized in that: The experimental rabbit compound feed comprises the feed additive according to any one of claims 1 or 2 and a basic feed; wherein, based on 100% of the mass of the compound feed, the weight proportion of the feed additive is 0.1-4%.

4. The experimental rabbit compound feed according to claim 3, characterized in that The basic feed comprises, by weight, 5-30 parts of corn, 7-35 parts of soybean hulls, 20-50 parts of alfalfa, 5-20 parts of soybean meal, 3-18 parts of wheat, 1-15 parts of bran, 0.1-1.5 parts of stone powder, 0.3-1.2 parts of salad oil, 0.1-2 parts of brewer's yeast powder, 0.5-4 parts of calcium hydrogen phosphate, 0.1-1 parts of feed-grade sodium chloride, and 1-5 parts of multivitamin and multi-mineral premix.

5. The experimental rabbit compound feed according to claim 3, characterized in that The experimental rabbit compound feed comprises, in parts by weight, 0.1-2 parts of feed additives, 10-20 parts of corn, 10-30 parts of soybean hulls, 30-40 parts of alfalfa, 10-15 parts of soybean meal, 5-15 parts of wheat, 1-10 parts of bran, 0.1-0.9 parts of stone powder, 0.5-1 parts of salad oil, 0.1-0.5 parts of brewer's yeast powder, 0.5-2 parts of calcium hydrogen phosphate, 0.1-0.5 parts of feed-grade sodium chloride, and 3-4 parts of multivitamin and multi-mineral premix; wherein the feed additives are composed of lime extract, roxburghii extract, polygonatum polysaccharide, radish seed, and Shenqu, and the weight ratio thereof is 1-2:1:1:1-2:

1.

6. The experimental rabbit compound feed according to claim 3, characterized in that The experimental rabbit compound feed includes, in parts by weight, 1 part of feed additives, 20 parts of corn, 10 parts of soybean hulls, 32 parts of alfalfa, 11 parts of soybean meal, 12 parts of wheat, 7 parts of bran, 0.6 parts of stone powder, 0.5 parts of soybean oil, 0.5 parts of brewer's yeast powder, 1 part of calcium hydrogen phosphate, 0.4 parts of feed-grade sodium chloride, and 4 parts of multivitamin and multi-mineral premix; wherein the feed additives are composed of lime extract, roxburghii extract, and polygonatum polysaccharide, and the weight ratio thereof is 1~2:1:

1.

7. The experimental rabbit compound feed according to claim 3, characterized in that Each kilogram of multivitamin premix contains vitamin A 150000-300000IU, vitamin D 1750-35000IU, vitamin E 1250-2500IU, vitamin K7.5-15mg, vitamin B1 175-350mg, vitamin B2 200-400mg, vitamin B6 150-300mg, vitamin B 12 0.5-1mg, niacin 1000-2000mg, pantothenic acid 300-600mg, folic acid 25-50mg, biotin 5-500mg, choline 25000-50000mg, iron 2500-5000mg, copper 225-450mg, manganese 1000-2000mg, zinc 1250-2500mg.

8. A use of the feed additive according to any one of claims 1 or 2, or the experimental rabbit compound feed according to any one of claims 3 to 7, characterized in that: The application is selected from at least one of the following applications: application in preparing products for promoting the growth and development of experimental rabbits, application in preparing products for maintaining the intestinal health of experimental rabbits, application in preparing products for enhancing the anti-stress ability of experimental rabbits, and application in preparing products for preventing or treating skin diseases of experimental rabbits.

9. The use according to claim 8, characterized in that The skin diseases of the experimental rabbits include skin diseases caused by fungal infections.

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