A novel protective agent for promoting healing of an ulcerated surface

Abstract

The application belongs to the technical field of medicine, and specifically discloses a novel protective agent for promoting ulcer surface healing. The novel protective agent for promoting ulcer surface healing comprises the following raw materials in parts by weight: 0.05-0.3 parts of hypochlorous acid, 0.02-0.08 parts of sodium chloride, 0.8-1.2 parts of modified brown algae extract and 0.2-0.6 parts of modified chitosan; wherein the concentration of the hypochlorous acid is 50-300 mg / L. The components in the novel protective agent prepared in the application cooperate with each other, jointly increase the structural strength after film formation, improve the form of the protective agent, so that the protective agent can be easily sprayed out, and the strength after film formation is relatively high.

Description

Technical Field

[0001] This application relates to the field of pharmaceutical technology, and in particular to a novel protective agent that promotes ulcer healing. Background Technology

[0002] Ulcers are generally caused by damage or microbial infection that breaks or removes the mucous membrane. They usually heal by the proliferation of the body's own cells, which takes a long time. They are usually treated with medication. Existing skin protectants for treating ulcers can be roughly divided into three types: wound dressings, wound protection gels, and wound protection sprays. Among them, wound protection sprays are popular because they are easy to use and do not affect limb movement.

[0003] The initial form of the wound protection spray is liquid. After being sprayed onto the skin for a period of time, it gradually gels and forms a film. It can isolate bacteria from the external environment for a certain period of time, promote wound healing, protect the wound while maintaining its breathability, promote wound repair, and prevent wound infection.

[0004] Existing wound protection sprays are mostly applied to the skin by spraying. Since film formation is also required, they are often difficult to spray due to excessive viscosity, or the liquid has good fluidity but insufficient film strength, which cannot effectively isolate bacteria. Summary of the Invention

[0005] To address the issue that excessively high or low viscosity of wound protection sprays negatively impacts their effectiveness, this application provides a novel protective agent that promotes ulcer healing.

[0006] This application provides a novel protective agent that promotes ulcer healing, employing the following technical solution:

[0007] A novel protective agent for promoting ulcer healing comprises, by weight, the following ingredients: 0.05-0.3 parts hypochlorous acid, 0.02-0.08 parts sodium chloride, 0.8-1.2 parts modified brown algae extract, and 0.2-0.6 parts modified chitosan; wherein the concentration of hypochlorous acid is 50-300 mg / L.

[0008] By adopting the above technical solution, hypochlorous acid has the ability to kill bacteria and resist biofilm. By releasing nascent oxygen [O], it denatures the proteins on bacteria and viruses, thereby killing pathogenic microorganisms, preventing skin infection, promoting wound healing, and effectively repairing skin ulcers to achieve the purpose of treating ulcers. Sodium chloride acts as a stabilizer to ensure that the solution system has good solubility and stability.

[0009] The active ingredients in modified brown algae extract have antioxidant and antiviral effects. They can not only activate skin cells and promote skin metabolism, but also have a certain viscosity. When combined with sodium chloride, it dissolves in blood and becomes sticky, which can block blood flow. It also has moisturizing and film-forming abilities, which can form a film on the skin surface to isolate bacteria and thus protect wounds.

[0010] Modified chitosan has excellent hemostatic properties, promotes wound healing, enhances wound skin metabolism, covers and isolates ulcers and wounds, protects wounds, prevents infection, and promotes the formation of granulation tissue. In addition, chitosan solution is viscous and has good film-forming properties. When mixed with modified brown algae extract, it not only adjusts the viscosity of the modified brown algae extract but also increases the structural strength of the film after formation, thereby improving the morphology of the protective agent, making it easy to spray out, and resulting in a film with high strength after formation.

[0011] Preferably, the preparation method of the modified brown algae extract includes the following steps:

[0012] (1) After washing and chopping the brown algae, soak it in citric acid for 30-45 minutes, filter and wash it with water, then add sodium bicarbonate, heat and stir for 1-2 hours to obtain mixture one;

[0013] (2) Disperse the mixture obtained in step (1) in ethanol, extract it at a temperature of 30-35℃, concentrate it under vacuum and filter it to obtain the original solution of brown algae extract.

[0014] (3) Mix the brown algae extract stock solution obtained in step (2), soybean dietary fiber and nano silver, and stir at 55-60℃ for 1-2 hours to obtain modified brown algae extract.

[0015] By adopting the above technical solution, brown algae are cleaned, and citric acid and sodium bicarbonate are added to improve the viscosity of the brown algae. Citric acid not only has a good bactericidal effect and can clean the bacteria in the brown algae, but also can adjust the pH of the system. Sodium bicarbonate is alkaline, and under the action of heating, it converts the water-insoluble alginate in the brown algae into water-soluble alkali metal salts, which helps to extract the original brown algae extract solution in the subsequent process.

[0016] Soybean dietary fiber and nano-silver were added to the extracted brown algae extract stock solution. Soybean dietary fiber has strong mechanical properties due to the uniformity of its supramolecular structure and the cross-linking effect of calcium ions between fiber macromolecules, resulting in strong intermolecular forces. The addition of soybean dietary fiber ensures its uniform dispersion within the brown algae extract stock solution system. Since the brown algae extract stock solution itself has a certain viscosity, it can effectively adsorb the soybean dietary fiber, thereby increasing the mechanical properties of the system. Nano-silver has antibacterial and wound-healing properties. The mixture of brown algae extract stock solution, soybean dietary fiber, and nano-silver not only enhances the mechanical properties of the system, resulting in better mechanical properties after film formation, but also increases the system's antibacterial and wound-healing effects.

[0017] In addition, soybean dietary fiber has a rough surface and a microporous structure. When nano-silver is loaded onto the surface of soybean dietary fiber, the soybean dietary fiber loaded with nano-silver and the brown algae extract stock solution not only increases the viscosity of the brown algae extract stock solution, but also helps the adsorption of nano-silver, so that nano-silver can fully exert its antibacterial and wound-protecting effects.

[0018] Preferably, the brown algae is any one or more of kelp, Sargassum fusiforme, sargassum, wakame, or Sargassum fusiforme.

[0019] By adopting the above technical solution, brown algae, in addition to nutrients such as protein, vitamins, sugars, and minerals, also contain active ingredients such as sea brown algae polysaccharides and brown algae polyphenols. The active ingredients in the modified brown algae extract have multiple effects such as enhancing the body's immunity, anti-oxidation, and antiviral properties. It can be used as a novel protective agent to promote ulcer healing and has good medicinal effects.

[0020] Preferably, the mass ratio of the brown algae extract stock solution, soybean dietary fiber and nano silver is 1:0.3-0.8:0.1-0.3.

[0021] By adopting the above technical solution and limiting the mass ratio of brown algae extract stock solution, soybean dietary fiber, and nano silver within a certain range, a modified brown algae extract with moderate viscosity and good film-forming properties is obtained. The combination of brown algae extract stock solution, soybean dietary fiber, and nano silver has a good effect, not only having antibacterial and wound-healing effects, but also regulating the viscosity and mechanical properties of brown algae extract stock solution, resulting in a novel protective agent with good film-forming properties and also having good medicinal effects.

[0022] Preferably, the pretreatment of soybean dietary fiber includes the following steps: dispersing soybean water-soluble dietary fiber in purified water, adding a sodium hydroxide solution with a mass concentration of 5-10%, stirring and keeping warm at 75-80℃ for 30-35 minutes, washing with ethanol, then dispersing it again in purified water, adding carboxymethyl cellulose, continuing to stir for 1-3 hours at a stirring rate of 450-550 rpm, drying, and pulverizing to 50-60 mesh to obtain pretreated soybean dietary fiber.

[0023] By adopting the above technical solution, soybean water-soluble dietary fiber is mixed with sodium hydroxide solution. The sodium hydroxide solution has a certain abrasive effect on the soybean water-soluble dietary fiber, making the surface of the soybean water-soluble dietary fiber rough and porous. Then, it is dispersed in purified water, and carboxymethyl cellulose is added. The aqueous solution of carboxymethyl cellulose has the functions of thickening, film formation, adhesion, water retention, colloidal protection, emulsification and suspension. Carboxymethyl cellulose coats the surface of soybean water-soluble dietary fiber. The treated soybean dietary fiber is then used in the preparation method of modified brown algae extract, which helps to improve the viscosity and film-forming properties of the preparation method of modified brown algae extract, and further improves the performance of the novel protective agent.

[0024] Preferably, the method for preparing the modified chitosan includes the following steps:

[0025] (1) Dissolve chitosan in acetic acid and stir for 20-30 min. Then add glutaraldehyde and react under microwave radiation for 1-2 h. Filter and wash to obtain pre-modified chitosan. The microwave radiation power is 400-450 W and the temperature is 35-40 °C.

[0026] (2) Dissolve the pre-modified chitosan obtained in step (1) in glacial acetic acid, then add white tea extract and activated carbon fiber, mix evenly, stir at 300-350 rpm for 35-45 min, filter, dry to obtain modified chitosan.

[0027] By adopting the above technical solution, chitosan contains a large number of active polar groups such as hydroxyl and amino groups, while glutaraldehyde is a crosslinking agent with two active carbonyl groups. It undergoes a condensation reaction with the amino or hydroxyl groups in chitosan to form a crosslinked structure, which improves the solubility and stability of chitosan and enhances its mechanical properties.

[0028] White tea extract has the effects of cooling blood and stopping bleeding, antibacterial and antioxidant properties. It can regulate the viscosity of the chitosan solution system, which helps to prepare it into a spray and facilitates the spraying of the protective agent. Activated carbon fiber not only has the effects of stopping bleeding, relieving pain, reducing inflammation and fighting infection, but also has a variety of functional groups on its surface. It can react with a variety of coagulation factors in the wound to activate the coagulation mechanism and accelerate the hemostasis effect. Moreover, the activated carbon fiber is uniformly dispersed in the white tea extract. When it is mixed with pre-modified chitosan, it increases the mechanical properties of the pre-modified chitosan and helps to improve the mechanical strength after film formation.

[0029] Preferably, the mass ratio of the pre-modified chitosan, white tea extract and activated carbon fiber obtained in step (1) is 1:0.5-0.9:0.1-0.3.

[0030] By adopting the above technical solution, the mass ratio of pre-modified chitosan, white tea extract, and activated carbon fiber is further limited to obtain modified chitosan with good solution state and superior mechanical properties after film formation. The pre-modified chitosan, white tea extract, and activated carbon fiber work together to adjust the viscosity and mechanical strength of the chitosan system, which helps to improve the mechanical properties and viscosity of the film formed by the surfactant when the modified chitosan is added to the surfactant.

[0031] Preferably, the chitosan has a viscosity range of 200-800 cp and a degree of deacetylation ≥90%.

[0032] Preferably, the preparation method of the white tea extract includes the following steps: pulverizing white tea leaves into 10-30 mesh powder, then placing them in purified water, extracting at a temperature of 95-100℃ for 25-30 minutes, filtering to obtain the extract, then adding pectinase, adjusting the pH of the enzymatic hydrolysate to 5.0-5.5 for extraction at a temperature of 50-55℃ for 60-65 minutes, inactivating the enzyme after enzymatic hydrolysis, adding mesoporous nano silica particles, and filtering to obtain the white tea extract.

[0033] By employing the above technical solution, white tea powder is extracted to obtain an extract. Pectinase can break down cell walls and release the components inside the cells, thereby increasing the extraction rate of tea. Mesoporous nano-silica particles have a large specific surface area. They are subsequently added to modified chitosan to prepare a novel protective agent, which can load antibacterial drugs and help load nano-silver particles, thus helping to exert the medicinal effects of nano-silver particles. At the same time, it helps to improve the viscosity and mechanical properties of white tea extract.

[0034] Secondly, this application also provides a method for preparing a novel protective agent that promotes ulcer healing, comprising the following steps: mixing hypochlorous acid, sodium chloride, modified brown algae extract, modified chitosan and purified water evenly to obtain a novel protective agent that promotes ulcer healing.

[0035] By adopting the above technical solution and preparation method, the raw materials are mixed evenly, the operation is simple, and a spray is made, which helps to spray out the spray and form a film at the wound site, thereby helping to isolate bacteria and exert the drug effect.

[0036] In summary, this application has the following beneficial effects:

[0037] 1. Hypochlorous acid in this application has the ability to kill bacteria and resist biofilm. By releasing nascent oxygen [O], it denatures the proteins on bacteria and viruses, thereby killing pathogenic microorganisms, preventing skin infection, promoting wound healing, and effectively repairing skin ulcers to achieve the purpose of treating ulcers. Sodium chloride acts as a stabilizer to ensure that the solution system has good solubility and stability.

[0038] 2. The active ingredients in the modified brown algae extract of this application have antioxidant and antiviral effects. They can not only activate skin cells and promote skin metabolism, but also have a certain viscosity. When sodium chloride comes into contact with blood, it dissolves and becomes viscous, which can block blood flow. It also has moisturizing and film-forming abilities, which can form a film on the skin surface to isolate bacteria and thus protect the wound.

[0039] 3. The modified chitosan in this application has good hemostatic properties, promotes wound healing, enhances wound skin metabolism, covers and isolates ulcers and wounds, protects the wound, prevents wound infection, and promotes the formation of wound granulation tissue. In addition, the chitosan solution is viscous and has good film-forming properties. When mixed with modified brown algae extract, it not only adjusts the viscosity of the modified brown algae extract, but also increases the structural strength of the film after formation, thereby improving the morphology of the protective agent, making it easy to spray out, and the film has high strength after formation. Detailed Implementation

[0040] The present application will be further described in detail below with reference to the embodiments.

[0041] The raw materials used in the examples and comparative examples are all commercially available. Among them, brown algae is kelp, and the viscosity range of chitosan is 200-800cp, and the degree of deacetylation is ≥90%.

[0042] Preparation example of modified brown algae extract

[0043] Preparation Example 1-1

[0044] The preparation method of modified brown algae extract includes the following steps:

[0045] (1) Wash and chop 2.5 kg of brown algae, soak it in 3 L of 10% citric acid for 35 min, filter and wash with water, then add 0.5 kg of sodium bicarbonate, heat and stir for 2 h to obtain mixture one;

[0046] (2) Disperse the mixture obtained in step (1) in 5L of ethanol, extract it at 35℃, concentrate it under vacuum and filter it to obtain the original solution of brown algae extract.

[0047] (3) The brown algae extract stock solution obtained in step (2), soybean dietary fiber and nano silver are mixed and stirred at 60°C for 2 hours to obtain modified brown algae extract; wherein, the mass ratio of brown algae extract stock solution, soybean dietary fiber and nano silver obtained in step (2) is 1:0.3:0.3.

[0048] The pretreatment of soybean dietary fiber includes the following steps: 0.5 kg of water-soluble soybean dietary fiber is dispersed in 1.5 L of purified water, 1.2 L of 10% sodium hydroxide solution is added, and the mixture is stirred and kept warm at 80 °C for 35 min. After washing with ethanol, the mixture is dispersed again in 2 L of purified water, 0.2 kg of carboxymethyl cellulose is added, and the mixture is stirred for 2 h at a stirring rate of 500 rpm. After drying, the mixture is pulverized to 60 mesh to obtain the pretreated soybean dietary fiber.

[0049] Preparation Examples 1-2

[0050] The difference from preparation example 1-1 is that soybean dietary fiber is not added in step (3).

[0051] Preparation Examples 1-3

[0052] The difference from preparation example 1-1 is that no nano-silver is added in step (3).

[0053] Preparation Examples 1-4

[0054] The difference from Preparation Example 1-1 is that the mass ratio of brown algae extract stock solution, soybean dietary fiber and nano silver is 1:0.8:0.1.

[0055] Preparation Examples 1-5

[0056] The difference from Preparation Example 1-1 is that the mass ratio of brown algae extract stock solution, soybean dietary fiber and nano silver is 1:0.1:0.6.

[0057] Preparation Examples 1-6

[0058] The difference from Preparation Example 1-1 is that sodium hydroxide solution is not added during the pretreatment of soybean dietary fiber.

[0059] Preparation Examples 1-7

[0060] The difference from Preparation Example 1-1 is that carboxymethyl cellulose is not added during the pretreatment of soybean dietary fiber.

[0061] Preparation example of modified chitosan

[0062] Preparation Example 2-1

[0063] The preparation method of modified chitosan includes the following steps:

[0064] (1) Dissolve 1.5 kg of chitosan in 2 L of acetic acid with a mass concentration of 5%, stir for 30 min, then add 0.3 kg of glutaraldehyde, react under microwave radiation for 2 h, filter, wash, and obtain pre-modified chitosan. The microwave radiation power is 450 W and the temperature is 40 °C.

[0065] (2) Dissolve the pre-modified chitosan obtained in step (1) in 2.5L of glacial acetic acid, then add white tea extract and activated carbon fiber, mix evenly, stir at 350rpm for 35min, filter, and dry to obtain modified chitosan; wherein, the mass ratio of the pre-modified chitosan, white tea extract and activated carbon fiber obtained in step (1) is 1:0.5:0.3; The preparation method of white tea extract includes the following steps: pulverize 2kg of white tea leaves into 30-mesh powder, then place them in 3L of purified water, extract at 95℃ for 30min, filter to obtain extract, then add 0.2kg of pectinase, adjust the pH of the enzyme hydrolysate to 5.0 for extraction, temperature 55℃, extraction time 65min, inactivate enzyme activity after enzymatic hydrolysis, then add 0.3kg of mesoporous nano silica particles, filter to obtain white tea extract.

[0066] Preparation Example 2-2

[0067] The difference from preparation example 2-1 is that white tea extract is not added in step (2).

[0068] Preparation Examples 2-3

[0069] The difference from preparation example 2-1 is that activated carbon fiber is not added in step (2).

[0070] Preparation Examples 2-4

[0071] The difference from Preparation Example 2-1 is that the mass ratio of the pre-modified chitosan, white tea extract and activated carbon fiber obtained in step (1) is 1:0.9:0.1.

[0072] Preparation Examples 2-5

[0073] The difference from Preparation Example 2-1 is that the mass ratio of the pre-modified chitosan, white tea extract and activated carbon fiber obtained in step (1) is 1:0.2:0.5.

[0074] Preparation Examples 2-6

[0075] The difference from Preparation Example 2-1 is that mesoporous nano-silica particles are not added in the preparation method of white tea extract.

[0076] Example

[0077] Example 1

[0078] A novel protective agent for promoting ulcer healing comprises, by weight, the following ingredients: 0.1 kg hypochlorous acid, 0.05 kg sodium chloride, 1.0 kg modified brown algae extract, 0.4 kg modified chitosan, and 1 L purified water; wherein the concentration of hypochlorous acid is 100 mg / L.

[0079] The preparation method of the above-mentioned pain-relieving ointment includes the following steps: mixing hypochlorous acid, sodium chloride, modified brown algae extract, modified chitosan and purified water evenly to obtain a novel protective agent that promotes ulcer healing.

[0080] The modified brown algae extract was prepared using Preparation Example 1-1; the modified chitosan was prepared using Preparation Example 2-1.

[0081] Example 2

[0082] A novel protective agent for promoting ulcer healing, which differs from Example 1 in that the modified brown algae extract was prepared using Preparation Examples 1-2.

[0083] Example 3

[0084] A novel protective agent for promoting ulcer healing, which differs from Example 1 in that the modified brown algae extract was prepared using Preparation Examples 1-3.

[0085] Example 4

[0086] A novel protective agent for promoting ulcer healing, which differs from Example 1 in that the modified brown algae extract was prepared using Preparation Examples 1-4.

[0087] Example 5

[0088] A novel protective agent for promoting ulcer healing, which differs from Example 1 in that the modified brown algae extract was prepared using Preparation Examples 1-5.

[0089] Example 6

[0090] A novel protective agent for promoting ulcer healing, which differs from Example 1 in that the modified brown algae extract was prepared using Preparation Examples 1-6.

[0091] Example 7

[0092] A novel protective agent for promoting ulcer healing, which differs from Example 1 in that the modified brown algae extract was prepared using Preparation Examples 1-7.

[0093] Example 8

[0094] A novel protective agent for promoting ulcer healing, which differs from Example 1 in that the modified chitosan is prepared using Preparation Example 2-2.

[0095] Example 9

[0096] A novel protective agent for promoting ulcer healing, which differs from Example 1 in that the modified chitosan is prepared using Preparation Examples 2-3.

[0097] Example 10

[0098] A novel protective agent for promoting ulcer healing, which differs from Example 1 in that the modified chitosan is prepared using Preparation Examples 2-4.

[0099] Example 11

[0100] A novel protective agent for promoting ulcer healing, which differs from Example 1 in that the modified chitosan is prepared using Preparation Examples 2-5.

[0101] Example 12

[0102] A novel protective agent for promoting ulcer healing, which differs from Example 1 in that the modified chitosan is prepared using Preparation Examples 2-6.

[0103] Example 13

[0104] A novel protective agent for promoting ulcer healing differs from Example 1 in that, by weight, it comprises the following raw materials: 0.05 kg hypochlorous acid, 0.08 kg sodium chloride, 1.2 kg modified brown algae extract, 0.6 kg modified chitosan, and 1 L purified water; wherein the concentration of hypochlorous acid is 50 mg / L.

[0105] Example 14

[0106] A novel protective agent for promoting ulcer healing differs from Example 1 in that, by weight, it comprises the following raw materials: 0.3 kg hypochlorous acid, 0.02 kg sodium chloride, 0.8 kg modified brown algae extract, 0.2 kg modified chitosan, and 1 L purified water; wherein the concentration of hypochlorous acid is 300 mg / L.

[0107] Comparative Example

[0108] Comparative Example 1

[0109] A novel protective agent for promoting ulcer healing differs from Example 1 in that, by weight, it comprises the following raw materials: 0.02 kg hypochlorous acid, 0.01 kg sodium chloride, 0.5 kg modified brown algae extract, 0.1 kg modified chitosan, and 1 L purified water; wherein the concentration of hypochlorous acid is 20 mg / L.

[0110] Comparative Example 2

[0111] A novel protective agent for promoting ulcer healing differs from Example 1 in that, by weight, it comprises the following raw materials: 0.5 kg hypochlorous acid, 0.12 kg sodium chloride, 1.6 kg modified brown algae extract, 0.8 kg modified chitosan, and 1 L purified water; wherein the concentration of hypochlorous acid is 500 mg / L.

[0112] Comparative Example 3

[0113] A novel protective agent for promoting ulcer healing, differing from Example 1 in that an equal amount of chitosan is used instead of modified chitosan.

[0114] Comparative Example 4

[0115] A novel protective agent that promotes ulcer healing, differing from Example 1 in that it does not contain modified chitosan.

[0116] Comparative Example 5

[0117] A novel protective agent that promotes ulcer healing, differing from Example 1 in that it does not contain modified brown algae extract.

[0118] Comparative Example 6

[0119] A novel protective agent for promoting ulcer healing, differing from Example 1 in that an equal amount of brown algae extract is used instead of modified brown algae extract.

[0120] Performance testing

[0121] The pain-relieving ointments prepared in Examples 1-14 and Comparative Examples 1-6 were subjected to relevant performance tests.

[0122] 1. Spray the new protective agent onto the sample plate and time the time it takes for the solution to stop flowing, which is the time it takes for the protective agent to form a film;

[0123] 2. Spray the new protective agent onto the sample plate. After the solution stops flowing and forms a film, perform a tensile test at 25 mm / min on a microcomputer-controlled electronic universal testing machine. Each sample should be tested at least 3 times, and the average value of the results should be taken.

[0124] The tensile strength of the novel protective agent is the ratio of the maximum tensile stress in film formation to the cross-sectional area of ​​the sample.

[0125] The elongation at break of the novel protective agent is the ratio of the deformed length of the film at fracture to its initial length; the test results are shown in Table 1.

[0126] Table 1 Test data for the examples and comparative examples

[0127]

[0128]

[0129] As can be seen from Table 1, the novel protective agents prepared in Examples 1, 4, 10 and 13-14 of this application have good viscosity and mechanical properties. When the novel protective agent is sprayed onto the sample plate, the solution stops flowing in the shortest time of 4 seconds, indicating that the solution has a fast film-forming time. The maximum tensile strength is 0.22 MPa, and the optimal elongation at break is 160%, indicating that the prepared novel protective agent has good mechanical properties after film formation. It can form a film on the skin surface, isolate bacteria, and thus achieve the effect of protecting the wound.

[0130] In Example 2, soybean dietary fiber was not added to the preparation method of the modified brown algae extract. As shown in Table 1, compared with Example 1, the time for the solution to stop flowing was longer, and the tensile strength and elongation at break were both smaller, indicating that soybean dietary fiber has strong mechanical properties and is uniformly dispersed in the brown algae extract stock solution system, thus increasing the mechanical properties of the brown algae extract stock solution system. In Example 3, nano-silver was not added to the preparation method of the modified brown algae extract. Compared with Example 1, the time for the solution to stop flowing did not change much, while the tensile strength and elongation at break were slightly smaller. In Example 5, the mass ratio of brown algae extract stock solution, soybean dietary fiber, and nano-silver was changed. Data tests showed that the tensile strength and elongation at break values ​​were better than those of Examples 2-3, but worse than those of Examples 1 and 4. This indicates that the mixture of brown algae extract stock solution, soybean dietary fiber, and nano-silver not only increased the mechanical properties of the system, resulting in better mechanical properties after subsequent film formation, but also increased the antibacterial and wound-healing effects of the system.

[0131] In Example 6, no sodium hydroxide solution was added during the pretreatment of soybean dietary fiber. In Example 7, no carboxymethyl cellulose was added during the pretreatment of soybean dietary fiber. As shown in Table 1, compared with Example 1, the time for the solution to stop flowing was longer, and the tensile strength and elongation at break were both lower. This indicates that the sodium hydroxide solution roughens the surface of the soybean water-soluble dietary fiber, which helps with the loading of subsequent components. The aqueous solution of carboxymethyl cellulose has thickening, film-forming, and adhesive effects, and can improve the viscosity of the brown algae extract.

[0132] In Example 8, no white tea extract was added to the preparation method of modified chitosan. In Example 9, no activated carbon fiber was added to the preparation method of modified chitosan. As shown in Table 1, compared with Example 1, the time when the solution stopped flowing, the tensile strength, and the elongation at break all changed significantly. This indicates that white tea extract can adjust the viscosity of the chitosan solution system. The subsequent mixing of activated carbon fiber with pre-modified chitosan increases the mechanical properties of the pre-modified chitosan and helps to improve the mechanical strength after film formation.

[0133] Example 11 changed the mass ratio of pre-modified chitosan, white tea extract and activated carbon fiber obtained in step (1). Data test showed that the tensile strength and elongation at break were better than those of Examples 8-9, but worse than those of Examples 1 and 10. This indicates that the pre-modified chitosan, white tea extract and activated carbon fiber work together to adjust the viscosity and mechanical strength of the chitosan system, which helps to improve the mechanical properties and viscosity of the film formed by the subsequent addition of modified chitosan to the surfactant.

[0134] In Example 12, the preparation method of white tea extract did not include mesoporous nano-silica particles. As shown in Table 1, compared with Example 1, the solution stopped flowing the fastest when the novel protective agent was sprayed on the sample plate, with a tensile strength of 0.17 MPa and an optimal elongation at break of 145%. This indicates that the mesoporous nano-silica particles were subsequently added to modified chitosan to prepare a novel protective agent, which can carry antibacterial drugs, help to load nano-silver particles, and also help to improve the viscosity and mechanical properties of white tea extract.

[0135] Comparative Examples 1-2 changed the amount of raw materials used in the novel protective agent. As shown in Table 1, compared with Example 1, the time when the solution stopped flowing was significantly slower, while the tensile strength and elongation at break were significantly smaller. This indicates that the novel protective agent has better comprehensive performance when the raw material components are mixed in a certain proportion. The change in the amount of each raw material affects the viscosity and mechanical properties of the novel protective agent after film formation.

[0136] Comparative Example 3 used an equal amount of chitosan instead of modified chitosan, and Comparative Example 6 used an equal amount of brown algae extract instead of modified brown algae extract. As shown in Table 1, compared with Example 1, the time for the solution to stop flowing was significantly slower, while the tensile strength and elongation at break were significantly smaller. This indicates that the modified chitosan or modified brown algae extract prepared in this application have good strength and viscosity, and can be subsequently applied to novel protective agents to improve the corresponding performance of the novel protective agents.

[0137] Comparative Example 4 did not add modified chitosan, and Comparative Example 5 did not add modified brown algae extract. As shown in Table 1, compared with Example 1, the time for the solution to stop flowing was significantly slower, while the tensile strength and elongation at break were significantly smaller. This indicates that both modified chitosan and modified brown algae extract affect the viscosity of the solution of the novel protective agent and the mechanical properties after film formation.

[0138] This specific embodiment is merely an explanation of this application and is not intended to limit it. After reading this specification, those skilled in the art can make modifications to this embodiment without contributing any inventive step, but such modifications are protected by patent law as long as they fall within the scope of the claims of this application.

Claims

1. A protective agent that promotes ulcer healing, characterized in that, By weight, it is composed of the following raw materials: 0.05-0.3 parts hypochlorous acid, 0.02-0.08 parts sodium chloride, 0.8-1.2 parts modified brown algae extract, and 0.2-0.6 parts modified chitosan; wherein the concentration of hypochlorous acid is 50-300 mg / L; The preparation method of the modified brown algae extract includes the following steps: (1) After washing and chopping the brown algae, soak it in citric acid for 30-45 minutes, filter and wash it with water, then add sodium bicarbonate, heat and stir for 1-2 hours to obtain mixture one; (2) Disperse the mixture obtained in step (1) in ethanol, extract it at a temperature of 30-35℃, concentrate it under vacuum and filter it to obtain the original solution of brown algae extract; (3) Mix the brown algae extract stock solution obtained in step (2), soybean dietary fiber and nano silver, and stir at 55-60℃ for 1-2 hours to obtain modified brown algae extract; The method for preparing the modified chitosan includes the following steps: (1) Dissolve chitosan in acetic acid and stir for 20-30 min. Then add glutaraldehyde and react under microwave radiation for 1-2 h. Filter and wash to obtain pre-modified chitosan. The microwave radiation power is 400-450 W and the temperature is 35-40 °C. (2) Dissolve the pre-modified chitosan obtained in step (1) in glacial acetic acid, then add white tea extract and activated carbon fiber, mix evenly, stir at 300-350 rpm for 35-45 min, filter, dry to obtain modified chitosan.

2. The protective agent for promoting ulcer healing according to claim 1, characterized in that, The brown algae mentioned are any one or more of kelp, Sargassum fusiforme, Sargassum fusiforme, Undaria pinnatifida, or Sargassum fusiforme.

3. The protective agent for promoting ulcer healing according to claim 1, characterized in that, The mass ratio of the brown algae extract stock solution, soybean dietary fiber and nano silver is 1:0.3-0.8:0.1-0.

3.

4. The protective agent for promoting ulcer healing according to claim 1, characterized in that, The pretreatment of soybean dietary fiber includes the following steps: dispersing soybean water-soluble dietary fiber in purified water, adding a sodium hydroxide solution with a mass concentration of 5-10%, stirring and keeping warm at 75-80℃ for 30-35 minutes, washing with ethanol, then dispersing it again in purified water, adding carboxymethyl cellulose, continuing to stir for 1-3 hours at a stirring rate of 450-550 rpm, drying, and pulverizing to 50-60 mesh to obtain pretreated soybean dietary fiber.

5. The protective agent for promoting ulcer healing according to claim 1, characterized in that, The mass ratio of the pre-modified chitosan, white tea extract and activated carbon fiber obtained in step (1) is 1:0.5-0.9:0.1-0.

3.

6. The protective agent for promoting ulcer healing according to claim 1, characterized in that, The chitosan has a viscosity range of 200-800 cp and a degree of deacetylation ≥90%.

7. The protective agent for promoting ulcer healing according to claim 1, characterized in that, The preparation method of the white tea extract includes the following steps: pulverizing white tea leaves into 10-30 mesh powder, then placing them in purified water, extracting at a temperature of 95-100℃ for 25-30 minutes, filtering to obtain the extract, then adding pectinase, adjusting the pH of the enzymatic hydrolysate to 5.0-5.5 for extraction at a temperature of 50-55℃ for 60-65 minutes, inactivating the enzyme after enzymatic hydrolysis, adding mesoporous nano silica particles, and filtering to obtain the white tea extract.

8. A method for preparing a protective agent for promoting ulcer healing according to any one of claims 1-7, characterized in that, The process includes the following steps: mixing hypochlorous acid, sodium chloride, modified brown algae extract, modified chitosan, and purified water until homogeneous to obtain a protective agent that promotes ulcer healing.

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