A method for constructing a double-signal sensing platform based on a non-copper nanoscale enzyme AuPt@PBA@L-Pd and application

By constructing a dual-signal sensing platform based on the non-copper nanozyme AuPt@PBA@L-Pd, and combining nanobodies and multifunctional nanozymes, the problems of low sensitivity and complexity in PEDV detection were solved, achieving high-sensitivity and low-cost visualization detection results.

CN117368134BActive Publication Date: 2026-06-26YUNNAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
YUNNAN UNIV
Filing Date
2023-10-10
Publication Date
2026-06-26

AI Technical Summary

Technical Problem

Existing technologies for detecting porcine epidemic diarrhea virus (PEDV) have low sensitivity, complex detection methods, and high equipment requirements, making it difficult to collect samples and measure them on-site in farms.

Method used

A dual-signal sensing platform based on the non-copper nanozyme AuPt@PBA@L-Pd was constructed. Nanobodies were used as enzyme-labeled antibodies, and a multifunctional nanocomposite material with oxidase-like, peroxidase-like, and laccase-like effects was combined to establish a visual colorimetric sensor. TMB and 2,4-DP were used as signal molecules for specific labeling.

Benefits of technology

It achieves highly sensitive, low-cost, and easy-to-operate PEDV detection, can intuitively display antigen content, improves the specificity and sensitivity of detection, and is suitable for applications in more fields.

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Abstract

The application provides a method for constructing a double-signal sensing platform based on a non-copper nanometer enzyme AuPt@PBA@L-Pd and application, and a multifunctional nanometer composite material AuPt@L-Pd@PBA (hereinafter referred to as APL) with the effects of an oxidase-like enzyme, a peroxidase-like enzyme and a laccase-like enzyme is prepared. The composite nanometer enzyme has a regular cubic morphology, takes PBA as a base material, loads L-Pd through an amide bond, simultaneously collects the advantages of the MOF material PBA and L-Pd, has multiple enzyme-like effects, and can catalyze different chromogenic substrates. The AuNPs and PtNPs on the surface of APL not only provide good binding sites for antibodies, but also successfully prepare a biological composite probe Ab2@APL, further amplify the sensing signal, and improve the sensitivity of the sensing platform. It is worth mentioning that APL has the effect of a laccase-like enzyme, but does not contain copper ions, and further improves the biocompatibility. Based on the high catalytic activity and multifunctional nanometer enzyme Ab2@APL, a multi-signal sensing platform can be constructed, and the application prospect in more fields is wider.
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