A pesticide composition for controlling citrus pests

Abstract

The present application belongs to the technical field of citrus pest control, and particularly relates to a pesticide composition for preventing and treating citrus pests. The pesticide composition for preventing and treating citrus pests is composed of bisdemethoxycurcumin and compound I at a mass ratio of 1-300:300-1, and the compound I is bifenazate, cyflumetofen or pyridaben. The pesticide composition of the present application has different action mechanisms of bisdemethoxycurcumin and other components on target pests, and the two components have a synergistic effect on citrus spider mites when compounded. Compared with a single effective component, the pesticide composition can improve the control effect on citrus spider mites, reduce the amount of pesticide used, reduce pesticide residues on citrus fruits, reduce environmental pollution, and provide support for seeking new and efficient pesticides for preventing and treating citrus spider mites.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of citrus pest control, and particularly relates to a pesticide composition for preventing and treating citrus pests. BACKGROUND

[0002] Panonychus citri is an important citrus pest, which is distributed in all citrus producing areas in China. P. citri has strong reproductive capacity and obvious generation alternation, and can occur comprehensively in some citrus producing areas. P. citri mainly sucks the sap of leaves, tender shoots, flower buds and fruits with adult mites and nymphs, among which tender leaves are most seriously harmed. P. citri mainly sucks the sap on the back of leaves, causing the leaves to lose green, and even to turn white, affect photosynthesis, and even to fall off, resulting in serious yield reduction.

[0003] At present, chemical pesticide control is still the main means for preventing and controlling P. citri, but long-term use of large amounts of chemical pesticides has caused the problem of P. citri resistance to become increasingly serious, which not only reduces the control effect of chemical pesticides, but also leads to environmental pollution. Therefore, it is necessary to continuously seek new and efficient pesticides to prevent and control P. citri.

[0004] Bisdemethoxycurcumin mainly exists in the tubers of plants of Zingiberaceae, Labiatae, Poaceae and Pedaliaceae, CAS No.: 33171-05-0, molecular formula: C 19 H 16 O4, and the structural formula is as follows

[0005]

[0006] Bisdemethoxycurcumin has pharmacological activities of anti-tumor, anti-inflammatory, anti-Alzheimer's disease, anti-protozoan and treatment of gastric ulcer. The prior art also reports that bisdemethoxycurcumin has activity against agricultural mites. Application No. CN200410021982.9 discloses a miticidal composition, a preparation method and a use, and specifically discloses in the specification that the curcumin compounds bisdemethoxycurcumin, demethoxycurcumin and curcumin all have contact killing activity against Tetranychus cinnabarinus, and bisdemethoxycurcumin has the strongest activity.

[0007] The information disclosed in this section of the background art is only intended to increase the understanding of the overall background of the present application, and should not be regarded as acknowledging or implying in any form that the information constitutes prior art known to those skilled in the art. SUMMARY

[0008] The present application aims to provide a pesticide composition for preventing and treating citrus pests, so as to solve the problem that the use of a large amount of chemical pesticides for a long time has caused the increasing drug resistance of Panonychus citri, which not only reduces the prevention and treatment effect of chemical pesticides on Panonychus citri, but also causes environmental pollution.

[0009] To achieve the above-mentioned object, the present application provides the following technical solutions.

[0010] The pesticide composition for preventing and treating citrus pests is composed of bisdemethoxycurcumin and compound I in a mass ratio of 1-300:300-1, and the compound I is bifenazate, cyflumetofen or pyridaben.

[0011] Preferably, when the compound I is bifenazate, the mass ratio of bisdemethoxycurcumin to compound I is 1-40:15-1.

[0012] Preferably, when the compound I is cyflumetofen, the mass ratio of bisdemethoxycurcumin to compound I is 1-30:20-1.

[0013] Preferably, when the compound I is pyridaben, the mass ratio of bisdemethoxycurcumin to compound I is 1-9:9-1.

[0014] Preferably, the citrus pests are Panonychus citri.

[0015] Compared with the prior art, the present application has the following beneficial effects:

[0016] The bisdemethoxycurcumin and other ingredients in the pesticide composition of the present application have different action mechanisms on target pests, and have a synergistic effect on Panonychus citri when compounded, so compared with a single effective ingredient, the present application can improve the prevention and treatment effect on Panonychus citri, reduce the amount of pesticide used, reduce pesticide residues on citrus fruits, reduce environmental pollution, and provide support for seeking new and efficient pesticides for preventing and treating Panonychus citri. DETAILED DESCRIPTION

[0017] The following clearly and completely describes the technical solutions of the present application, and obviously, the described embodiments are part of the embodiments of the present application, rather than all the embodiments.

[0018] Example : Indoor biological test

[0019] 1. Test agents: 98% bisdemethoxycurcumin (Wuhan Costan Biotechnology Co., Ltd.), 97% bifenazate technical (Aristech Biotech Co., Ltd.), 98% cyflumetofen technical (Shandong Lianhe Pesticide Industry Co., Ltd.), and 95% pyridaben technical (Inner Mongolia Bailin Technology Co., Ltd.).

[0020] The single dose stock solution was prepared by dissolving the original drug with dimethyl sulfoxide and diluting with 0.1% Tween-80 aqueous solution. Multiple groups of mixtures were prepared. Each single dose and each group of mixtures had six concentration gradients set by equal difference method.

[0021] 2. Test insect: Panonychus citri was collected from citrus orchard and fed with fresh clean citrus leaves in artificial incubator at temperature (25±1)℃, relative humidity RH (75±5)%, and photoperiod 16L:8D for multiple generations. Female adult mites with consistent physiological state were selected as test insects.

[0022] 3. Test method: (Reference: Guidelines on Laboratory Test of Pesticides, Insecticide, Part 12: Leaf Mite Glass Slide Immersion Method)

[0023] The double-sided tape was cut into 2 cm long and pasted on one end of the glass slide. The back of the test insects was pasted on the double-sided tape, two rows of 15 insects each on each glass slide, a total of 30 insects. The glass slide was placed in a container padded with wet sponge and covered with a lid, and placed at (25±1)℃. After 2h, the dead and injured individuals were removed and 30 insects were added to each glass slide. The glass slide was immersed in the drug solution and shaken gently for 5s, then the excess drug solution was absorbed with water-absorbing paper and placed in a white porcelain disk padded with wet sponge, covered with a plastic film with good light transmission. Each treatment was repeated 4 times, and a 0.1% Tween-80 aqueous solution treatment was set as a blank control. The test insects were placed in an artificial incubator at (25±1)℃, relative humidity RH (75±5)%, and photoperiod 16L:8D. After 48h, the death of the test insects was investigated, and the total number of insects and the number of dead insects in each treatment were recorded, and the corrected mortality rate of each treatment was calculated. The mite was considered dead if it did not move when touched with a brush.

[0024]

[0025] In the above formula: P--mortality rate, unit %; K--dead insect number; N--total insect number.

[0026]

[0027] In the above formula: P1--corrected mortality rate, unit %; P t treatment mortality rate, unit %; P0--blank control mortality rate, unit %.

[0028] 4. Data analysis

[0029] The logarithmic values of the concentration of each treatment and the probability values of the corrected mortality rate were subjected to regression analysis to calculate the LC 50Then, the synergistic coefficient (CTC value) of the mixture is calculated according to the Sun Yunpei method, and the synergistic effect of the pesticide is evaluated according to the calculated synergistic coefficient (CTC), CTC≤80 is antagonistic effect, 80<CTC<120 is additive effect, and CTC≥120 is synergistic effect. The experimental results are shown in Tables 1-3.

[0030] Table 1 Indoor bioactivity determination of bisdemethoxycurcumin and bifenthrin complex on Panonychus citri

[0031] Medicament name and ratio LC 50 (mg / L) ATI TTI CTC Bisdemethoxycurcumin 143.7625 100.0000 -- -- Bifenazate 11.3157 1270.4693 -- -- Bisdemethoxycurcumin 1 : Bifenazate 15 9.7715 1471.2429 1197.3150 122.8785 Bisdemethoxycurcumin 1 : Bifenazate 10 6.6058 2176.3072 1164.0630 186.9578 Bisdemethoxycurcumin 1 : Bifenazate 5 11.0365 1302.6095 1075.3911 121.1289 Bisdemethoxycurcumin 1 : Bifenazate 3 10.2749 1399.1620 977.8520 143.0853 Bisdemethoxycurcumin 1 : Bifenazate 1 16.7173 859.9624 685.2347 125.4990 Bisdemethoxycurcumin 3 : Bifenazate 1 26.8592 535.2449 392.6173 136.3274 Bisdemethoxycurcumin 5 : Bifenazate 1 40.5370 354.6451 295.0782 120.1868 Bisdemethoxycurcumin 10 : Bifenazate 1 27.1673 529.1748 206.4063 256.3753 Bisdemethoxycurcumin 15 : Bifenazate 1 18.4334 779.9022 173.1543 450.4087 Bisdemethoxycurcumin 20 : Bifenazate 1 13.7021 1049.2005 155.7366 673.7018 Bisdemethoxycurcumin 30 : Bifenazate 1 35.2504 407.8323 137.7571 296.0518 Bisdemethoxycurcumin 40 : Bifenazate 1 47.8536 300.4215 128.5480 233.7037

[0032] As shown in Table 1, the synergistic coefficients of bisdemethoxycurcumin and bifenthrin in the mass ratio of 1-40:15-1 are all greater than 120, that is, the bioactivity of bisdemethoxycurcumin and bifenthrin after complexing shows synergistic effect on Panonychus citri.

[0033] Table 2 Indoor bioactivity determination of bisdemethoxycurcumin and cyflumetofen complex on Panonychus citri

[0034] Medicament name and ratio LC 50 (mg / L) ATI TTI CTC Bisdemethoxycurcumin 143.7625 100.0000 -- -- Clofentezine 3.0269 4749.4962 -- -- Bisdemethoxycurcumin 1 : Clofentezine 20 2.3749 6053.4128 4528.0916 133.6857 Bisdemethoxycurcumin 1 : Clofentezine 15 1.8110 7938.2938 4458.9027 178.0325 Bisdemethoxycurcumin 1 : Clofentezine 10 2.7226 5280.3386 4326.8147 122.0375 Bisdemethoxycurcumin 1 : Clofentezine 5 1.7573 8180.8741 3974.5802 205.8299 Bisdemethoxycurcumin 1 : Clofentezine 3 2.5416 5656.3779 3587.1221 157.6857 Bisdemethoxycurcumin 1 : Clofentezine 1 4.5688 3146.6140 2424.7481 129.7708 Bisdemethoxycurcumin 3 : Clofentezine 1 7.1153 2020.4700 1262.3740 160.0532 Bisdemethoxycurcumin 5 : Clofentezine 1 13.0679 1100.1194 874.9160 125.7400 Bisdemethoxycurcumin 10 : Clofentezine 1 19.3787 741.8583 522.6815 141.9332 Bisdemethoxycurcumin 15 : Clofentezine 1 26.9519 533.4040 390.5935 136.5624 Bisdemethoxycurcumin 20 : Clofentezine 1 36.8144 390.5062 321.4046 121.4999 Bisdemethoxycurcumin 30 : Clofentezine 1 46.6833 307.9527 249.9837 123.1891

[0035] As shown in Table 2, the synergistic coefficients of bisdemethoxycurcumin and cyflumetofen in the mass ratio of 1-30:20-1 are all greater than 120, that is, the bioactivity of bisdemethoxycurcumin and cyflumetofen after complexing shows synergistic effect on Panonychus citri.

[0036] Table 3 Indoor bioactivity determination of bisdemethoxycurcumin and pyridaben complex on Panonychus citri

[0037] Medicament name and ratio LC 50 (mg / L) ATI TTI CTC Bisdemethoxycurcumin 143.7625 100.0000 -- -- Pyridaben 18.1246 793.1899 -- -- Bisdemethoxycurcumin 1 : Pyridaben 9 15.1424 949.4037 723.8709 131.1565 Bisdemethoxycurcumin 1 : Pyridaben 7 13.2290 1086.7224 706.5412 153.8088 Bisdemethoxycurcumin 1 : Pyridaben 5 12.3716 1162.0364 677.6583 171.4782 Bisdemethoxycurcumin 1 : Pyridaben 3 18.9207 759.8160 619.8924 122.5722 Bisdemethoxycurcumin 1 : Pyridaben 1 21.3781 672.4756 446.5950 150.5784 Bisdemethoxycurcumin 3 : Pyridaben 1 42.6052 337.4295 273.2975 123.4660 Bisdemethoxycurcumin 5 : Pyridaben 1 51.4198 279.5859 215.5317 129.7192 Bisdemethoxycurcumin 7 : Pyridaben 1 36.6182 392.5985 186.6487 210.3408 Bisdemethoxycurcumin 9 : Pyridaben 1 57.5863 249.6471 169.3190 147.4418

[0038] As shown in Table 3, the synergistic coefficients of bisdemethoxycurcumin and pyridaben in the mass ratio of 1-9:9-1 are all greater than 120, that is, the bioactivity of bisdemethoxycurcumin and pyridaben after complexing shows synergistic effect on Panonychus citri.

[0039] In summary, the action mechanisms of bisdemethoxycurcumin and other ingredients in the pesticide composition of the present application on target pests are different, and the two have synergistic effect on Panonychus citri when complexed, compared with a single effective ingredient, the control effect on Panonychus citri can be improved, the amount of pesticide used can be reduced, the pesticide residues on the citrus fruits can be reduced, and the environmental pollution can be reduced.

[0040] The foregoing description of specific exemplary embodiments of the application has been presented for the purposes of illustration and description. It is not intended to be exhaustive or to limit the application to the precise forms disclosed, and obviously many modifications and variations are possible in light of the above teaching. It is intended that the scope of the application be limited not with this detailed description, but rather by the claims appended hereto.

Claims

1. A pesticide composition for controlling citrus pests, characterized in that, It consists of bisdemethoxycurcumin and compound I, which is diphenylhydramine, in a mass ratio of 1-40:15-1.

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