A space compression chromatography experimental cabinet

Abstract

This invention discloses a spatial compression chromatography cabinet, involving a chromatography column, including a cabinet body. The cabinet body is equipped with a loading stage and a pressurization device. The loading stage is located below the pressurization device and is mounted on a sliding rail. The cabinet body is divided into a chromatography experimental area and a loading area by a loading partition and a recovery partition. The chromatography column is located in the chromatography experimental area. In this invention, except for manual placement of experimental materials, automatic feeding and automatic selection of feed rate are possible. Both stationary and mobile phase experiments can be automatically set and freely switched. The closed-loop experiment eliminates concerns about contamination and potential harm to operators, reducing labor costs. The automated experiment improves experimental efficiency. It achieves chromatography, elution, and recovery within a closed system, reducing the evaporation of organic solvents into the air, reducing solvent consumption, and minimizing harm to operators.

Description

Technical Field

[0001] This invention relates to the field of chromatography technology, and more particularly to a space compression chromatography experimental cabinet. Background Technology

[0002] Chromatography is a method for separating and determining multi-component mixtures based on the different stationary or mobile phase states of each component within a chromatography column. It is commonly used for the analysis of compounds or metal ions. A chromatography cabinet is a special-purpose cryogenic cabinet specifically designed for chromatography experiments, used for experiments requiring a low-temperature environment, refrigeration of items, and constant-temperature experiments of various components.

[0003] Existing chromatography cabinets mostly adopt an integrated storage and experimental structure. However, when dealing with the chromatography of different reagents and components, most rely on manual preparation and testing, leading to a decrease in the constant temperature within the cabinet. Furthermore, manual experiments are prone to significant errors and contamination, potentially harming both the chromatography cabinet and personnel. Current laboratories and chromatography cabinets utilize semi-open chromatography systems, where manual intervention is required during the chromatography process. This results in the volatilization of toxic organic solvents released during the experiment, causing substantial harm to personnel. Summary of the Invention

[0004] The main objective of this invention is to provide a space compression chromatography chamber, which aims to solve the existing technical problems.

[0005] To achieve the above objectives, the present invention provides a space compression chromatography cabinet, relating to a chromatography column, including a cabinet body, wherein a loading stage and a pressurization device are provided inside the cabinet body, the loading stage is located below the pressurization device and is mounted on a sliding rail, the interior of the cabinet body is divided into a chromatography experimental area and a loading area by a loading partition and a recovery partition, and the chromatography column is located in the chromatography experimental area.

[0006] Furthermore, the loading assembly includes a first loading platform, a second loading platform, and a third loading platform. The first loading platform has rotating shafts on both sides, which are connected to one end of a first push rod. The other end of the first push rod is connected to the second loading platform. The second loading platform has compression buttons on both sides of its front and rear ends. The compression buttons on both sides facing the third loading platform are connected to one end of the second push rod. The other end of the second push rod is connected to the third loading platform through the rotating shaft.

[0007] Furthermore, one end of the first push rod is provided with a rotating shaft hole connected to the rotating shaft, and the other end is provided with a receiving groove for connecting the compression button, and the inside is provided with a push shaft groove adapted to the second push rod.

[0008] Furthermore, one end of the third push rod is provided with a rotating shaft hole connected to the rotating shaft, and the other end is provided with a receiving groove for connecting the compression button, and the inside is provided with a push shaft groove adapted to the second push rod.

[0009] Furthermore, the pressurizing device includes a pressure-drawing slide rail with a pressure-drawing device mounted on it. The pressure-drawing device has a pressure-drawing pipe inside, and the pressure-drawing pipe is connected to a fixed pipe through a flexible connecting pipe.

[0010] Furthermore, the pressurizing device also includes a slide rail plate on which multiple movable material tanks are mounted. The material tanks are connected to a fixed pipe through a top discharge port. A telescopic pressurizing gun is mounted at the bottom of the slide rail plate and is connected to the fixed pipe through a flexible connecting pipe.

[0011] Furthermore, a drive shaft is installed in the middle of the conversion partition, the drive shaft is connected to the telescopic rod and can be driven to rotate, the front end of the telescopic rod is provided with a connecting gun, and the upper part of the connecting gun is provided with an injection port.

[0012] Furthermore, the chromatography column is provided with an injection tube at the upper end, an observation screen at the front end, and a recovery hole at the lower part.

[0013] Furthermore, the movable slide rail includes a straight slide rail and an arc-shaped slide rail, with one end of the straight slide rail extending above the chromatography column.

[0014] The beneficial effects of this invention are reflected in:

[0015] In this invention, apart from the manual placement of experimental materials, the feeding and feed rate can be automatically selected. The experiments with either the stationary phase or the mobile phase can be automatically set and freely switched. The closed experiment eliminates the need to consider the pollution generated during the experiment and the harm to personnel, reducing labor costs. The automated experiment improves experimental efficiency. It realizes chromatography, elution, and recovery in a closed system, reducing the volatilization of organic solvents in the air, reducing solvent consumption, and reducing harm to operators. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the initial state of the present invention;

[0017] Figure 2 This is a schematic diagram of the loading platform structure of the present invention;

[0018] Figure 3 This is a schematic diagram of the loading platform structure of the present invention. Figure 2 ;

[0019] Figure 4 This is a schematic diagram of the first push rod structure of the present invention;

[0020] Figure 5 This is a schematic diagram of the second push rod structure of the present invention;

[0021] Figure 6 This is a schematic diagram of the third push rod structure of the present invention;

[0022] Figure 7 This is a schematic diagram of the conversion partition structure of the present invention;

[0023] Figure 8 This is a schematic diagram of the pressurization device of the present invention;

[0024] Figure 9 This is a schematic diagram of the pressurization device of the present invention. Figure 2 ;

[0025] Figure 10 This is a schematic diagram of the chromatography column structure of the present invention.

[0026] Explanation of reference numerals in the attached figures

[0027] 1. Cabinet; 101. Drug loading compartment; 102. Recycling compartment; 103. Moving slide rail; 2. Conversion compartment; 201. Drive shaft; 202. Connecting gun; 203. Injection port; 204. Telescopic rod; 3. Drug loading platform; 301. Injection hole; 302. First loading platform; 303. First push rod; 304. Second loading platform; 305. Second push rod; 306. Third loading platform; 307. Third push rod 308. Feed rod; 309. Rotating shaft; 310. Compression button; 311. Rotating shaft hole; 312. Feed shaft groove; 313. Receiving groove; 4. Pressurizing device; 401. Pressure extractor slide rail; 402. Pressure extractor; 403. Pressure extraction pipe; 404. Slide rail plate; 405. Material tank; 406. Fixed pipe; 407. Pressurizing gun; 5. Chromatography column; 501. Observation screen; 502. Injection pipe; 503. Recovery hole. Detailed Implementation

[0028] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of the present invention, and not all of them. Unless otherwise specified, the embodiments and features in the embodiments of this application can be combined with each other. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0029] Please see Figure 1 This invention provides a space compression chromatography experimental cabinet, involving a chromatography column 5, including a cabinet body 1, a loading platform 3 and a pressurizing device 4 inside the cabinet body 1, the loading platform 3 being located below the pressurizing device 4 and mounted on a sliding rail 103, the interior of the cabinet body 1 being divided into a chromatography experimental area and a loading area by a loading partition 101 and a recovery partition 102, the chromatography column 5 being located in the chromatography experimental area.

[0030] This embodiment, apart from the manual placement of experimental materials, can automatically feed materials, automatically select the feed rate, and automatically set and switch between stationary and mobile phase experiments. It adopts a closed experiment, eliminating the need to consider the pollution generated by the experiment and the harm to personnel, reducing labor costs, and the automated experiment improves experimental efficiency. It realizes chromatography, elution, and recovery in a closed system, reducing the volatilization of organic solvents in the air, reducing solvent consumption and harm to operators.

[0031] In one embodiment, please refer to Figure 2-6 The loading assembly includes a first loading platform 302, a second loading platform 304, and a third loading platform 306. The first loading platform 302 has rotating shafts 308 on both sides, which are connected to one end of a first push rod 303. The other end of the first push rod 303 is connected to the second loading platform 304. The second loading platform 304 has compression buttons 309 on both its front and rear ends. The compression buttons 309 on both sides facing the third loading platform 306 are connected to one end of a second push rod 305. The other end of the second push rod 305 is connected to the third loading platform 306 via the rotating shafts 308. Each of the first loading platform 302, the second loading platform 304, and the third loading platform 306 has a filling hole 301 for installing a sealing ring.

[0032] This embodiment addresses the limitation of existing laboratories, which, due to the large number of experimental materials and the space constraints of the reagent tanks, cannot automate the injection of multiple materials in the chromatography experimental area. This leads to repetitive work, material contamination, significant waste of work efficiency, and the potential for errors. The embodiment, however, enables the injection of multiple materials, simplifies and speeds up the operation, and improves work efficiency.

[0033] Specifically, the first loading platform 302, the second loading platform 304, and the third loading platform 306 move forward sequentially under the drive of the moving slide rail 103. After the first loading platform 302 completes its work, it moves along the moving slide rail 103 to the arc-shaped slide rail section. The second loading platform 304 automatically separates from the first loading platform 302 without external force intervention. The subsequent third loading platform 306 and second loading platform 304 also automatically separate, realizing automatic operation of various materials.

[0034] In one embodiment, please refer to Figure 4 The first push rod 303 has a rotating shaft hole 310 at one end that is connected to the rotating shaft 308, and a receiving groove 312 at the other end for connecting the compression button 309. The inside has a push shaft groove 311 that is adapted to the second push rod 305.

[0035] In one embodiment, please refer to Figure 6The third push rod 307 has a rotating shaft hole 310 at one end that is connected to the rotating shaft 308, and a receiving groove 312 at the other end for connecting the compression button 309. The inside has a push shaft groove 311 that is adapted to the second push rod 305.

[0036] The rear end of the second push rod 305 is a rotating shaft hole 310, which connects to the compression button 309 at the front end of the second loading platform 304.

[0037] The structural arrangement of the first push rod 303, the second push rod 305 and the third push rod 307 allows the first loading platform 302, the second loading platform 304 and the third loading platform 306 to be tightly connected to reduce space occupation, and can be automatically separated during use, making it convenient and quick to use.

[0038] In one embodiment, please refer to Figure 8 and Figure 9 The pressurizing device 4 includes a pressure-drawing slide rail 401, on which a pressure-drawing device 402 is provided. The pressure-drawing device 402 has a pressure-drawing pipe 403 inside, and the pressure-drawing pipe 403 is connected to the fixed pipe 406 through a flexible connecting pipe.

[0039] In one embodiment, please refer to Figure 8 and Figure 9 The pressurizing device 4 also includes a slide rail plate 404, on which multiple movable material tanks 405 are provided. The material tanks 405 are connected to a fixed pipe 406 through a top discharge port. A telescopic pressurizing gun 407 is provided at the bottom of the slide rail plate 404 and is connected to the fixed pipe 406 through a flexible connecting pipe.

[0040] After the loading platform 3 is set up, the gas in each medicine tank is gradually extracted from the rotating shaft hole 310 and sent into the fixed pipe 406 by the movement of the vacuum pump 402 on the vacuum pump slide rail 401 and the vacuum pipe 403. At this time, the slide rail plate 404 moves the pressure tank in the material tank 405 and connects it to the fixed pipe 406 to send the extracted gas into the pressure tank for later use. Through the free switching of the vacuum pumping device, the integrated automatic feeding and discharging speed is realized, which improves the experimental efficiency.

[0041] In one embodiment, please refer to Figure 7 A drive shaft 201 is installed in the middle of the conversion partition 2. The drive shaft 201 is connected to the telescopic rod 204 and can drive it to rotate. A connecting gun 202 is provided at the front end of the telescopic rod 204, and a filling port 203 is provided on the upper part of the connecting gun 202.

[0042] When the first column of the loading platform controlled by the moving slide rail 103 reaches above the connecting gun 202, as Figure 7As shown, the connecting gun 202 is rotated by the drive shaft 201 to reach below the injection hole 301 at the bottom of the first drug tank in the first column. The telescopic rod 204 extends and retracts the connecting gun 202 so that the injection port 203 at the top of the connecting gun 202 coincides with the injection hole 301 at the bottom of the drug tank. Thus, the connecting gun 202 connects the injection hole 301 at the bottom of the drug tank and the injection tube 502 of the chromatography column 5 at the same time.

[0043] In one embodiment, please refer to Figure 10 The chromatography column 5 has an injection tube 502 at the upper end, an observation screen 501 at the front end, and a recovery hole 503 at the lower part.

[0044] In one embodiment, the movable slide rail 103 includes a straight slide rail and an arc-shaped slide rail, with one end of the straight slide rail extending above the chromatography column 5.

[0045] The specific working principle of this chromatography cabinet is as follows:

[0046] First, there are three loading platforms on the loading platform 3. The third loading platform 306 accommodates a part of the second push rod 305 through the push shaft groove 311 of the third push rod 307, and compresses a part of the rotation shaft 308 of the second push rod 305, which is the compression button 309 of the second loading platform 304, so that the third push rod 307 can smoothly accommodate a part of the second push rod 305 by relying on the push shaft groove 311.

[0047] Subsequently, the first loading platform 302 also accommodates a part of the second push rod 305 by relying on the push shaft groove 311 of the first push rod 303. Similarly, the compression button 309 at the rear end of the second loading platform 304 is compressed to smoothly accommodate a part of the second push rod 305 by relying on the push shaft groove 311. This arrangement is to ensure that the three material platforms are tightly attached to each other before operation, compressing the space while still ensuring the diversity of chromatography materials.

[0048] After the loading platform 3 is set up, the gas in each medicine tank is gradually extracted from the rotating shaft hole 310 and sent into the fixed pipe 406 by the movement of the pressure extractor 402 on the pressure extractor slide rail 401 and the pressure extraction pipe 403. At this time, the slide rail plate 404 moves the pressure tank in the material tank 405 and connects it to the fixed pipe 406 to send the extracted gas into the pressure tank for later use.

[0049] The staff then removed the required experimental components from the drug loading compartment 101 and sequentially injected them into the third loading platform 306, the second loading platform 304, and the first loading platform 302 on the drug loading platform 3 through the injection hole 301 into the chromatography column 5. The partition door of the drug loading area of ​​the cabinet 1 was then closed, and the work began.

[0050] When the moving slide rail 103 controls the first column of the third loading platform 306 to reach above the connecting gun 202, as follows Figure 7 As shown, the connecting gun 202 is rotated by the drive shaft 201 to reach below the injection hole 301 at the bottom of the first drug tank in the first column. The telescopic rod 204 extends and retracts the connecting gun 202 so that the injection port 203 at the top of the connecting gun 202 coincides with the injection hole 301 at the bottom of the drug tank. Thus, the connecting gun 202 connects the injection hole 301 at the bottom of the drug tank and the injection tube 502 of the chromatography column 5 at the same time.

[0051] After the material inside the third loading platform 306 is filled, the moving slide rail 103 drives it to move upward. At the same time, the third push rod 307 will gradually disengage from the second push rod 305. When the front push shaft groove 311 of the third push rod 307 leaves the second push rod 305, the receiving groove 312 will be blocked by the spring-back compression button 309 during the disengagement process. While fixing the distance between the two, the third loading platform 306 can rely on the compression button 309 of the second loading platform 304 as the rotation axis 308, so that it can more smoothly come to the upper part of the moving slide rail 103.

[0052] At this time, the first column of medicine canisters on the second loading platform 304 comes above the connecting gun 202. Repeat the previous steps. After the work is completed, when the second loading platform 304 moves upward along the moving slide rail 103, the second push rod 305 of the second loading platform 304 will gradually disengage from the push shaft groove 311 of the first push rod 303. When it is completely disengaged, the rear compression button 309 rebounds and locks the receiving groove 312 of the first push rod 303. Relying on the first push rod 303 as the rotation axis 308, it comes above the moving slide rail 103.

[0053] After the last column of the drug tanks on the first loading platform 302 has finished filling, the pressure gun 407 uses the tank itself and the connecting gun 202 as a pressure boosting pipe to start micro-pressurizing the inside of the chromatography column 5 to start the stationary phase chromatography experiment, or to start the fast mobile phase chromatography experiment by pressurizing at full speed.

[0054] At this time, the staff only needs to stand outside the partition door and record the experiment by referring to the observation screen 501. After the chromatography experiment is completed, the experimental components sent into the recovery device through the recovery hole 503 are collected again and the experiment is repeated after elution is completed.

[0055] Once the experimental components are extracted from the chromatography column 5, the slide rail 404 controls the elution tank in the material tank 405 to connect to the fixed pipe 406. The pressure gun 407 can quickly elute the contents of the chromatography column, which is convenient, fast, and improves experimental efficiency.

[0056] Alternatively, the loading platform of the loading stage 3 can be returned to the experimental starting position by setting the moving slide rail 103, and then the chromatography material can be re-injected into the loading stage 3. Then, the flexible connecting tube can be connected to the lower part of the pressure gun 407 and the injection port 203 of the connecting gun 202 for direct elution. This will reduce some of the risk of contamination and prevent the eluent from contaminating the loading tank, which is the connecting part.

[0057] It should be noted that if the embodiments of the present invention involve directional indicators such as up, down, left, right, front, back, etc., the directional indicators are only used to explain the relative positional relationship and movement of the components in a certain specific posture (as shown in the figure). If the specific posture changes, the directional indicators will also change accordingly.

[0058] Furthermore, if the embodiments of this invention involve descriptions such as "first" or "second," these descriptions are for descriptive purposes only and should not be construed as indicating or implying their relative importance or implicitly specifying the number of technical features indicated. Therefore, a feature defined with "first" or "second" may explicitly or implicitly include at least one of those features. Additionally, the meaning of "and / or" throughout the text includes three parallel solutions; for example, "A and / or B" includes solution A, solution B, or a solution where both A and B are satisfied simultaneously. Furthermore, "multiple" refers to two or more. Moreover, the technical solutions of the various embodiments can be combined with each other, but this must be based on the ability of those skilled in the art to implement them. When the combination of technical solutions is contradictory or cannot be implemented, such a combination of technical solutions should be considered non-existent.

[0059] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A space compression chromatography experimental cabinet, relating to a chromatography column (5), including a cabinet body (1), characterized in that: The cabinet (1) is equipped with a loading platform (3) and a pressurizing device (4). The loading platform (3) is located below the pressurizing device (4) and is located on a sliding rail (103). The interior of the cabinet (1) is divided into a chromatography experimental area and a loading area by a loading partition (101) and a recovery partition (102). The chromatography column (5) is located in the chromatography experimental area. The loading platform (3) includes a first loading platform (302), a second loading platform (304), and a third loading platform (306). The first loading platform (302) has rotating shafts (308) on both sides, which are connected to one end of a first push rod (303) through the rotating shafts (308). The other end of the first push rod (303) is connected to the second loading platform (304). The second loading platform (304) has compression buttons (309) on both sides of its front and rear ends. The compression buttons (309) on both sides facing the third loading platform (306) are connected to one end of a second push rod (305). The other end of the second push rod (305) is connected to the third loading platform (306) through the rotating shafts (308). The first push rod (303) has a rotating shaft hole (310) at one end that is connected to the rotating shaft (308), and a receiving groove (312) at the other end for connecting the compression button (309). The push rod (303) has a push shaft groove (311) inside that is adapted to the second push rod (305). The third push rod (307) has a rotating shaft hole (310) at one end that connects to the rotating shaft (308) on the third loading platform (306), and a receiving groove (312) at the other end for connecting the compression button (309) on the second loading platform (304). The push rod (307) has a push shaft groove (311) inside that is adapted to the second push rod (305). The pressurizing device (4) includes a pressure-drawing slide rail (401), on which a pressure-drawing device (402) is provided. The pressure-drawing device (402) has a pressure-drawing pipe (403) inside, and the pressure-drawing pipe (403) is connected to a fixed pipe (406) through a flexible connecting pipe. The pressurizing device (4) also includes a slide rail plate (404) on which multiple movable material tanks (405) are provided. The material tanks (405) are connected to a fixed pipe (406) through a top discharge port. A telescopic pressurizing gun (407) is provided at the bottom of the slide rail plate (404) and is connected to the fixed pipe (406) through a flexible connecting pipe.

2. The space compression chromatography chamber as described in claim 1, characterized in that: A drive shaft (201) is installed in the middle of the conversion partition (2) inside the cabinet (1). The drive shaft (201) is connected to the telescopic rod (204) and can be driven to rotate. A connecting gun (202) is provided at the front end of the telescopic rod (204), and a filling port (203) is provided on the upper part of the connecting gun (202).

3. The space compression chromatography chamber as described in claim 1, characterized in that: The chromatography column (5) has an injection tube (502) at the upper end, an observation screen (501) at the front end, and a recovery hole (503) at the lower part.

4. The space compression chromatography chamber as described in claim 1, characterized in that: The movable slide rail (103) includes a straight slide rail and an arc-shaped slide rail, with one end of the straight slide rail extending above the chromatography column (5).

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