A treatment method for improving estrus rate of cows

Abstract

The present invention provides a treatment method for improving the estrus rate of cows, comprising the following steps: S1, preparing a daily diet for cows to provide sufficient nutrition for the cows; S2, injecting L-citrulline into the cows at the same time as the first intramuscular injection of gonadorelin into the cows; S3, injecting prostaglandin into the cows intramuscularly for the second time on the 5th to 7th day after the first injection, and injecting L-citrulline into the cows again; S4, injecting gonadorelin into the cows intramuscularly for the third time on the 8th to 9th day after the first injection, and injecting L-citrulline into the cows again. The present invention increases the estrus rate of cows, improves the reproductive efficiency, and increases the benefits of cattle farms by intraperitoneally injecting L-citrulline into cows based on the technology of intramuscular injection of exogenous gonadotropins.

Description

Technical Field

[0001] The invention relates to a processing method, in particular to a processing method for improving the estrus rate of cows, and belongs to the technical field of livestock breeding. Background Art

[0002] Estrus is a periodic estrus phenomenon in cows after they reach sexual maturity, which is called the estrus cycle. There are two ways to calculate the estrus cycle, and both methods produce the same result: one is the interval from the start of one estrus to the start of the next, and the other is the interval from the end of one estrus to the end of the next. An estrus cycle for dairy cows lasts 19-22 days, with an average of about 21 days.

[0003] Synchronized estrus and timed insemination have become a common technique used in breeding cow farms to improve the reproductive performance of cows. This technique allows cows to be in estrus within a certain period of time, allowing for centralized artificial breeding at a predetermined time, greatly improving the efficiency of staff and the profitability of the farm. The principle is mainly to inject exogenous gonadotropin-releasing hormone (GnRH) into the muscle, prompting the pituitary gland to release follicle-stimulating hormone (FSH) or luteinizing hormone (LH). These hormones act on the target organ, the ovary, to promote the development of follicles and thus increase the estrus rate of cows.

[0004] At present, this technology is widely used, but in actual production, the estrus rate of cows is about 60%, which is still relatively low. Especially when the nutritional conditions are insufficient and the cows are thin, the estrus rate of cows is even lower. Therefore, a treatment method for improving the estrus rate of cows is proposed. Summary of the Invention

[0005] In view of this, the present invention provides a method for improving the estrus rate of cows to solve or alleviate the technical problems existing in the prior art and at least provide a beneficial choice.

[0006] The technical solution of the embodiment of the present invention is achieved as follows: A method for improving the estrus rate of cows comprises the following steps:

[0007] S1. Prepare daily diet for cows to provide them with adequate nutrition;

[0008] S2. Inject L-citrulline into the cow at the same time as the first intramuscular injection of gonadorelin.

[0009] S3. 5-7 days after the first injection, the cows were given a second intramuscular injection of prostaglandin and an additional injection of L-citrulline.

[0010] S4. Eight to nine days after the first injection, the cows were given three intramuscular injections of gonadorelin and an additional injection of L-citrulline.

[0011] S5. On the 10th to 12th day after the first injection, the cows were artificially inseminated.

[0012] Further preferably, in S1, the ingredients of the cow diet include straw, silage, yellow rice, corn, cottonseed meal and premix.

[0013] More preferably, in S2-4, L-citrulline is injected intraperitoneally.

[0014] More preferably, in S2-4, the injection amount of L-citrulline is 0.022-0.032 mg / kg.

[0015] The embodiment of the present invention adopts the above technical solution, which has the following advantages: the present invention increases the estrus rate of cows, improves the reproductive efficiency, and increases the benefits of the cattle farm by intraperitoneally injecting L-citrulline into cows based on the technology of intramuscular injection of exogenous gonadotropin.

[0016] The above summary is for illustrative purposes only and is not intended to be limiting in any way. In addition to the illustrative aspects, embodiments and features described above, further aspects, embodiments and features of the present invention will be readily apparent by reference to the accompanying drawings and the following detailed description. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or technical descriptions. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0018] Figure 1 is a flow chart of the steps of the present invention;

[0019] Figure 2 This is a nutritional composition diagram of the cow diet of the present invention;

[0020] Figure 3 The effect of L-citrulline injection on estrus rate of cows;

[0021] Figure 4 This is the effect of L-citrulline injection on the reproductive hormone levels of cows on the 10th day. DETAILED DESCRIPTION

[0022] Hereinafter, only certain exemplary embodiments are briefly described. As will be appreciated by those skilled in the art, the described embodiments may be modified in various ways without departing from the spirit or scope of the present invention. Therefore, the drawings and description are to be considered as illustrative in nature and not restrictive.

[0023] The embodiments of the present invention are described in detail below with reference to the accompanying drawings.

[0024] Example 1

[0025] like Figure 1-4 As shown, an embodiment of the present invention provides a method for improving the estrus rate of cows, comprising the following steps:

[0026] S1. Prepare daily diet for cows to provide them with adequate nutrition;

[0027] S2. Inject L-citrulline into the cow at the same time as the first intramuscular injection of gonadorelin.

[0028] S3. On the 5th day after the first injection, the cows were given a second intramuscular injection of prostaglandin and an additional injection of L-citrulline.

[0029] S4. Eight days after the first injection, the cows were given three intramuscular injections of gonadorelin and L-citrulline.

[0030] S5. On the 12th day after the first injection, the cows were artificially inseminated.

[0031] In one embodiment, in S1, the ingredients of the cow diet include straw, silage, yellow rice, corn, cottonseed meal and premix; and the nutritional components in the cow diet are improved to provide the cow with adequate nutrition.

[0032] In one embodiment, in S2-4, L-citrulline is injected intraperitoneally; intraperitoneal injection of the cow ensures the cow's absorption of L-citrulline.

[0033] In one embodiment, in S2-4, the injection amount of L-citrulline is 0.022 mg / kg; the injection amount of L-citrulline is controlled according to the weight of the cow.

[0034] Example 2

[0035] like Figure 1-4 As shown, an embodiment of the present invention provides a method for improving the estrus rate of cows, comprising the following steps:

[0036] S1. Prepare daily diet for cows to provide them with adequate nutrition;

[0037] S2. Inject L-citrulline into the cow at the same time as the first intramuscular injection of gonadorelin.

[0038] S3. Six days after the first injection, the cows were given a second intramuscular injection of prostaglandin and an additional injection of L-citrulline.

[0039] S4. Nine days after the first injection, the cows were given three intramuscular injections of gonadorelin and L-citrulline again.

[0040] S5. On the 11th day after the first injection, the cows were artificially inseminated.

[0041] In one embodiment, in S1, the ingredients of the cow diet include straw, silage, yellow rice, corn, cottonseed meal and premix; and the nutritional components in the cow diet are improved to provide the cow with adequate nutrition.

[0042] In one embodiment, in S2-4, L-citrulline is injected intraperitoneally; intraperitoneal injection of the cow ensures the cow's absorption of L-citrulline.

[0043] In one embodiment, in S2-4, the injection amount of L-citrulline is 0.032 mg / kg; the injection amount of L-citrulline is controlled according to the weight of the cow.

[0044] Example 3

[0045] like Figure 1-4 As shown, an embodiment of the present invention provides a method for improving the estrus rate of cows, comprising the following steps:

[0046] S1. Prepare daily diet for cows to provide them with adequate nutrition;

[0047] S2. Inject L-citrulline into the cow at the same time as the first intramuscular injection of gonadorelin.

[0048] S3. Seven days after the first injection, the cows were given a second intramuscular injection of prostaglandin and an additional injection of L-citrulline.

[0049] S4. Nine days after the first injection, the cows were given three intramuscular injections of gonadorelin and L-citrulline again.

[0050] S5. On the 10th day after the first injection, the cows were artificially inseminated.

[0051] In one embodiment, in S1, the ingredients of the cow diet include straw, silage, yellow rice, corn, cottonseed meal and premix; and the nutritional components in the cow diet are improved to provide the cow with adequate nutrition.

[0052] In one embodiment, in S2-4, L-citrulline is injected intraperitoneally; intraperitoneal injection of the cow ensures the cow's absorption of L-citrulline.

[0053] In one embodiment, in S2-4, the injection amount of L-citrulline is 0.027 mg / kg; the injection amount of L-citrulline is controlled according to the weight of the cow.

[0054] In one embodiment, Figure 3 As shown, compared with the control group, the estrus rate of cows in the experimental group was significantly higher than that in the control group (P < 0.05), and increased by 72.73% compared with the control group.

[0055] In one embodiment, Figure 4 As shown in the results, compared with the control group, the levels of gonadotropin-releasing hormone (GnRH), follicle-stimulating hormone (FSH), luteinizing hormone (LH) and estrogen (E2) in the serum of cows were significantly increased, which were increased by 18.83% (P < 0.05), 83.87% (P < 0.05), 78.00% (P < 0.05) and 39.12% (P < 0.05) respectively.

[0056] The above description is merely a specific embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any person skilled in the art can easily conceive of various modifications and substitutions within the technical scope disclosed in the present invention, and such modifications and substitutions are intended to be within the scope of protection of the present invention. Therefore, the scope of protection of the present invention shall be subject to the scope of protection of the claims.

Claims

1. A method for increasing the estrus rate of cows, characterized in that: The following steps are involved: S1. Prepare daily diet for cows to provide them with adequate nutrition; S2. Inject L-citrulline into the cow at the same time as the first intramuscular injection of gonadorelin. S3. 5-7 days after the first injection, the cows were given a second intramuscular injection of prostaglandin and an additional injection of L-citrulline. S4. Eight to nine days after the first injection, the cows were given three intramuscular injections of gonadorelin and another injection of L-citrulline. S5. Perform artificial insemination on the cows 10-12 days after the first injection. In S1, the ingredients of the cow diet include straw, silage, yellow storage, corn, cottonseed meal and premix; In S2-4, the injection amount of L-citrulline is 0.022-0.032 mg / kg.

2. The method for increasing the estrus rate of cows according to claim 1, characterized in that: In the above-mentioned S2-4, L-citrulline is injected intraperitoneally.

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