A method for improving the liquid level detection and liquid suction and discharge performance of a pipette

Abstract

The present application relates to the technical field of pipettes and provides a method for improving the liquid level detection and liquid suction and discharge performance of a pipette, wherein the pipette includes a gun tip and a TIP head connected to the gun tip, a piston is provided inside the gun tip, a filter core is provided inside the TIP head, a first chamber is formed between the piston and the filter core, and the filter core is divided into a standard filter core and a filter core to be tested. The method provided in the present application uses the relevant operating parameters of the pipette when the filter core inside the TIP head is a standard filter core as a benchmark, and can effectively identify the type of the filter core to be tested inside the TIP head when the pipette is subsequently used, and calculates the movement speed of the piston adapted to the filter core to be tested according to the type of the filter core to be tested, so that when the density of the filter core to be tested is different from the density of the standard filter core, the air pressure change inside the first chamber is kept as consistent as possible, thereby facilitating improving the accuracy of the pipette when using filter cores of different densities for liquid level detection and liquid suction and discharge.

Description

Technical Field

[0001] The present application relates to the technical field of pipettes, and in particular to a method for improving the liquid level detection and liquid aspiration and discharge performance of a pipette. Background Art

[0002] The contents of this section merely provide background information related to this application and may not constitute prior art.

[0003] Currently, pneumatic pipettes on the market generally have functions such as liquid level detection and pressure anomaly detection. The principle is to rely on internal air pressure sensors to obtain air pressure changes for judgment. Specifically, the tip of the pipette is connected to a TIP head, which has a piston inside the gun head, and a chamber is formed between the end of the TIP head and the piston. When actually detecting the liquid level, the pipette appropriately performs suction and exhaust actions (i.e., controls the movement of the piston in the chamber), and relies on the air pressure sensor to obtain the air pressure change data in the above chamber in real time, so as to compare the obtained air pressure change data with the preset air pressure change threshold, and then determine whether the end of the TIP head is in contact with the liquid surface.

[0004] In related technologies, in order to improve the performance of the pipette, the pipette usually uses a TIP head with a filter element for aspiration and discharge of liquid. At this time, the filter element further divides the above-mentioned chamber into a first chamber between the filter element and the piston and a second chamber between the filter element and the end of the TIP head. When the pipette detects the liquid level or aspirates and discharges liquid, air will pass through the filter element and circulate between the first chamber and the second chamber.

[0005] However, due to the varying density of filters inside TIP tips on the market, the permeability of the filters to air varies significantly. Specifically, the greater the density of the filter, the worse the permeability, while the smaller the density, the better the permeability. Consequently, when the pipette performs an aspiration or blowing action, the varying density of the filter will result in varying amounts of air entering and exiting the first chamber, leading to varying pressure changes within the first chamber. Therefore, if only one pressure change threshold is set, when the density of the filter inside the TIP tip changes, accurate liquid level detection based on the pressure change within the first chamber will be impossible. Furthermore, varying density of the filter inside the TIP tip will also affect the accuracy of the pipette during aspiration and discharge. Summary of the Invention

[0006] In view of this, the purpose of the present application is to provide a method for improving the liquid level detection and liquid suction and discharge performance of a pipette. This method uses the relevant operating parameters of the pipette when the filter element inside the TIP head is a standard filter element as a benchmark. When the pipette is used subsequently, the type of filter element to be tested inside the TIP head can be effectively identified, and the movement speed of the piston adapted to the filter element to be tested is calculated according to the type of filter element to be tested. Therefore, when the density of the filter element to be tested is different from that of the standard filter element, the air pressure change inside the first chamber is kept as consistent as possible, which is conducive to improving the accuracy of the pipette when using filter elements of different densities for liquid level detection and liquid suction and discharge.

[0007] The purpose of this application is achieved through the following technical solutions:

[0008] A method for improving the liquid level detection and liquid aspiration and discharge performance of a pipette, wherein the pipette includes a pipette tip and a tip connected to the pipette tip, wherein a piston is provided inside the pipette tip, a filter element is provided inside the tip, a first chamber is formed between the piston and the filter element, and the filter element is divided into a standard filter element and a test filter element. The method comprises the following steps:

[0009] S1. Obtaining first air pressure change data inside the first chamber when the filter element is the standard filter element, and using the first air pressure change data as standard air pressure change data;

[0010] S2. Obtaining the second air pressure change data inside the first chamber when the filter element is the filter element to be tested;

[0011] S3. Comparing the second air pressure change data with the standard air pressure change data to determine whether the filter element to be tested is the standard filter element. If the filter element to be tested is not the standard filter element, executing step S4;

[0012] S4. Calculate the movement speed of the piston that is compatible with the current filter element to be tested based on the proportional relationship between the standard filter element and the filter element to be tested.

[0013] In some possible embodiments, in step S4, the proportional relationship between the standard filter element and the filter element to be tested includes a first proportional relationship and a second proportional relationship;

[0014] The first proportional relationship is the proportional relationship between the standard filter element and the filter element to be tested when the pipette performs an aspiration action, and is expressed as:

[0015]

[0016] The second proportional relationship is the proportional relationship between the standard filter element and the filter element to be tested when the pipette performs the blowing action, and is expressed as:

[0017]

[0018] In the above formulas (1) and (2), V represents the volume of the first chamber in the initial state, n represents the amount of air in the first chamber in the initial state, Δt represents the movement time of the piston, S represents the cross-sectional area of ​​the first chamber, v1 represents the movement speed of the piston when the filter element is the standard filter element, v2 represents the movement speed of the piston when the filter element is the filter element to be tested, m1 represents the air circulation rate per unit time of the standard filter element, and m2 represents the air circulation rate per unit time of the filter element to be tested.

[0019] In some possible embodiments, the derivation process of the first proportional relationship is:

[0020] Assume that the air flow rate per unit time of the standard filter element is m1, and the air flow rate per unit time of the filter element to be tested is m2;

[0021] Then, during the time Δt, the amount of the substance in the air entering the first chamber through the standard filter element is:

[0022] △n1=m1△t (3),

[0023] The amount of substance in the air entering the first chamber through the filter element to be tested is:

[0024] △n2=m2△t (4);

[0025] According to the ideal gas state equation,

[0026] (P-△P1)(V+△V1)=(n+△n1)RT (5),

[0027] (P-△P2)(V+△V2)=(n+△n2)RT (6),

[0028] In the above formulas (5) and (6), P represents the air pressure of the first chamber in the initial state, R represents the molar gas constant, T represents the temperature, ΔP1 represents the air pressure change value of the first chamber within Δt when the filter element is the standard filter element, ΔP2 represents the air pressure change value of the first chamber within Δt when the filter element is the filter element to be tested, ΔV1 represents the volume change value of the first chamber within Δt when the filter element is the standard filter element, and ΔV2 represents the volume change value of the first chamber within Δt when the filter element is the filter element to be tested;

[0029] Assuming the cross-sectional area of ​​the first chamber is S, we have:

[0030] △V1=v1△tS (7),

[0031] △V2=v2ΔtS (8),

[0032] Let P-ΔP1=P-ΔP2, then:

[0033]

[0034] Substituting the above equations (3), (4), (7) and (8) into equation (9) respectively, the first proportional relationship equation can be obtained.

[0035] In some possible embodiments, the derivation process of the second proportional relationship is:

[0036] Assume that the air flow rate per unit time of the standard filter element is m1, and the air flow rate per unit time of the filter element to be tested is m2;

[0037] Then, during the time Δt, the amount of substance in the air flowing out of the first chamber through the standard filter element is:

[0038] Δn1=m1Δt (10),

[0039] The amount of substance in the air flowing out of the first chamber through the filter element to be tested is:

[0040] Δn2=m2Δt (11);

[0041] According to the ideal gas state equation,

[0042] (P+ΔP1)(V-ΔV1)=(n-Δn1)RT (12),

[0043] (P+ΔP2)(V-ΔV2)=(n-Δn2)RT (13),

[0044] In the above formulas (12) and (13), P represents the air pressure of the first chamber in the initial state, R represents the molar gas constant, T represents the temperature, ΔP1 represents the air pressure change value of the first chamber within Δt when the filter element is the standard filter element, ΔP2 represents the air pressure change value of the first chamber within Δt when the filter element is the filter element to be tested, ΔV1 represents the volume change value of the first chamber within Δt when the filter element is the standard filter element, and ΔV2 represents the volume change value of the first chamber within Δt when the filter element is the filter element to be tested;

[0045] Assuming the cross-sectional area of ​​the first chamber is S, we have:

[0046] ΔV1=v1ΔtS (14),

[0047] ΔV2=v2ΔtS (15),

[0048] Let P + ΔP1 = P + ΔP2, then:

[0049]

[0050] Substituting the above equations (10), (11), (14) and (15) into equation (16) respectively, the second proportional relationship equation can be obtained.

[0051] In some possible embodiments, in step S1, the first air pressure change data is obtained by the pipette performing an action of suctioning, blowing, or piercing the TIP head.

[0052] In some possible embodiments, in step S2, the second air pressure change data is obtained by the pipette performing an action of suctioning, blowing, or piercing the TIP head.

[0053] The technical solutions of the embodiments of the present application have at least the following advantages and beneficial effects:

[0054] The method provided in the present application uses the relevant operating parameters of the pipette when the filter element inside the TIP head is a standard filter element as a benchmark. When the pipette is subsequently used, the type of the filter element to be tested inside the TIP head can be effectively identified, and the movement speed of the piston adapted to the filter element to be tested can be calculated according to the type of the filter element to be tested. As a result, when the density of the filter element to be tested is different from that of the standard filter element, the air pressure change inside the first chamber remains as consistent as possible, thereby facilitating improved accuracy in liquid level detection and aspiration and discharge when the pipette uses filter elements of different densities. BRIEF DESCRIPTION OF THE DRAWINGS

[0055] Figure 1 A partial cross-sectional view of a pipette provided for some embodiments of the present application;

[0056] Figure 2 A waveform diagram of the air pressure inside the first chamber when the pipette provided in some embodiments of the present application uses a standard filter element and performs suction and blowing actions;

[0057] Figure 3 A waveform diagram of the air pressure inside the first chamber when the pipette provided in some embodiments of the present application uses a filter element to be tested that is not a standard filter element and performs suction and blowing actions;

[0058] Figure 4 The pipette provided in some embodiments of the present application uses a filter element to be tested that is not a standard filter element and corrects the movement speed of the piston to perform suction and blowing actions. The air pressure waveform inside the first chamber is shown.

[0059] Icon: 10- gun tip, 20- tip head, 30- piston, 40- filter element, 50- first chamber. DETAILED DESCRIPTION

[0060] In order to make the purpose, technical solutions and advantages of this application clearer, the technical solutions of this application will be clearly and completely described below in conjunction with specific implementation methods.

[0061] A partial cross-sectional view of a known pipette is shown in FIG. Figure 1 As shown, such a pipette generally includes a gun tip 10 and a TIP head 20 connected to the gun tip 10. Furthermore, a piston 30 is provided inside the gun tip 10, a filter core 40 is provided inside the TIP head 20, and a first chamber 50 is formed between the piston 30 and the filter core 40. When the pipette performs an aspiration action, the piston 30 moves in a direction away from the filter core 40 to aspirate air outside the first chamber 50 into the first chamber 50. Correspondingly, when the pipette performs an air blowing action, the piston 30 moves in a direction close to the filter core 40 to expel air inside the first chamber 50 to the outside of the first chamber 50.

[0062] At the same time, the pipette may also include an air pressure sensor (not shown in the figure) and a control module (not shown in the figure), wherein the air pressure sensor is used to detect the air pressure inside the first chamber 50 and send air pressure detection information, and the control module can receive the air pressure detection information sent by the air pressure sensor and generate air pressure change data inside the first chamber 50 (that is, the air pressure change value of the first chamber 50 after the pipette performs actions such as suction and blowing).

[0063] However, in combination with the content described in the background technology, it can be seen that during actual use of this type of pipette, when the density of the filter element 40 inside the TIP head 20 is different, the air pressure change inside the first chamber 50 is different each time the pipette performs an aspiration or blowing action. On this basis, if only one air pressure change threshold is set, it will not be possible to accurately detect the liquid level based on the air pressure change in the first chamber 50, and the accuracy of the pipette when aspirating or discharging liquid will be affected.

[0064] To this end, the present application further divides the filter element 40 inside the TIP head 20 into a standard filter element and a test filter element. The standard filter element refers to a filter element 40 whose specific density is known in advance before the actual use of the pipette and whose corresponding operating parameters of the pipette are obtained after actual use in the pipette (to be explained below), so that the relevant operating parameters of the pipette corresponding to the standard filter element can be used as a benchmark; the test filter element refers to the filter element 40 actually used in the subsequent use of the pipette.

[0065] Secondly, the present application provides a method for improving the liquid level detection and liquid aspiration and discharge performance of a pipette, the method comprising the following steps:

[0066] S1. Acquire first air pressure change data inside the first chamber 50 when the filter element 40 is a standard filter element, and use the first air pressure change data as standard air pressure change data.

[0067] It is understandable that, in step S1 , the first air pressure change data can be obtained by the pipette performing an action of suctioning, blowing, or piercing the TIP head 20 .

[0068] Specifically, when the filter element 40 is a standard filter element, the air pressure value inside the first chamber 50 is first detected by the air pressure sensor and sent to the control module, and then the pipette is controlled to perform the action of suction, blowing or piercing the TIP head 20. During this process, the air pressure inside the first chamber 50 will change. After the pipette completes the action of suction, blowing or piercing the TIP head 20, the air pressure sensor detects the air pressure value inside the first chamber 50 again and sends it to the control module. At this time, the control module can obtain the air pressure change value inside the first chamber 50 before and after the pipette performs the action of suction, blowing or piercing the TIP head 20 based on the air pressure values ​​sent twice by the air pressure sensor. The air pressure change value is the first air pressure change data.

[0069] It should be noted that the above-mentioned action of the pipette to pierce the TIP head 20 refers to the process of the pipette connecting the TIP head 20 to the gun tip 10 under the drive of the Z-axis drive mechanism. In the process of piercing the TIP head 20, the air inside the first chamber 50 will pass through the filter element 40 and flow out to the outside of the first chamber 50. Therefore, the action of the pipette to pierce the TIP head 20 can be equivalent to the pipette performing the blowing action.

[0070] On this basis, by setting the relevant operating parameters of the pipette when the filter element 40 is a standard filter element, the pipette can have the best liquid level detection and suction and discharge performance, wherein the relevant operating parameters may include the movement speed of the piston 30.

[0071] S2. Obtain second air pressure change data inside the first chamber 50 when the filter element 40 is the filter element to be tested.

[0072] It is understood that in step S2, the second air pressure change data can also be obtained by the pipette performing an action of suctioning, blowing, or piercing the TIP head 20. The method for obtaining the second air pressure change data is the same as the method for obtaining the first air pressure change data, and will not be repeated here.

[0073] S3. Compare the second air pressure change data with the standard air pressure change data to determine whether the filter element to be tested is a standard filter element. If the filter element to be tested is not a standard filter element, execute step S4.

[0074] Specifically, based on the first air pressure change data, if the second air pressure change data is equal to the first air pressure change data, it means that the filter element to be tested is a standard filter element. At this time, the pipette's piston 30 only needs to move at a preset movement speed (that is, the movement speed of the pipette's piston 30 when the filter element 40 is a standard filter element) so that the pipette performs an air suction or blowing action, so that the pipette can have the best liquid level detection and suction and discharge performance.

[0075] Conversely, if the second air pressure change data is not equal to the first air pressure change data, it indicates that the filter element being tested is not a standard filter element. Furthermore, if the second air pressure change data is greater than the first air pressure change data, it indicates that the filter element being tested is a high-density filter element with a higher density than the standard filter element. If the second air pressure change data is less than the first air pressure change data, it indicates that the filter element being tested is a low-density filter element with a lower density than the standard filter element. In this case, step S4 is executed.

[0076] S4. If the filter element to be tested is not a standard filter element, the control module calculates the movement speed of the piston 30 that is compatible with the current filter element to be tested based on the proportional relationship between the standard filter element and the filter element to be tested.

[0077] It can be understood that when the filter element to be tested is not a standard filter element, by executing step S4, the movement speed of the piston 30 of the pipette can be corrected, so that even if the filter element to be tested is not a standard filter element, when the pipette performs the suction or blowing action, the air pressure change inside the first chamber 50 can be kept as consistent as possible with the air pressure change inside the first chamber 50 when the filter element 40 is a standard filter element. On this basis, the pipette only needs to pre-set an air pressure change threshold, so that the pipette can have good liquid level detection and suction and discharge performance when targeting filter elements of different densities.

[0078] Specifically, in some embodiments of the present application, in step S4, the proportional relationship between the standard filter element and the filter element to be tested includes a first proportional relationship and a second proportional relationship;

[0079] The first proportional relationship is the proportional relationship between the standard filter element and the filter element to be tested when the pipette performs an aspiration action. In other words, the movement speed of the piston 30 adapted to the current filter element to be tested when the pipette performs an aspiration action can be calculated based on the first proportional relationship. Specifically, the first proportional relationship is expressed as:

[0080]

[0081] Correspondingly, the second proportional relationship is the proportional relationship between the standard filter element and the filter element to be tested when the pipette performs the blowing action. In other words, the movement speed of the piston 30 adapted to the current filter element to be tested when the pipette performs the blowing action can be calculated based on the second proportional relationship. Specifically, the second proportional relationship is expressed as:

[0082]

[0083] It should be noted that, in the above formulas (1) and (2), V represents the volume of the first chamber 50 in the initial state, n represents the amount of air in the first chamber 50 in the initial state, Δt represents the movement time of the piston 30, S represents the cross-sectional area of ​​the first chamber 50, v1 represents the movement speed of the piston 30 when the filter element 40 is a standard filter element, v2 represents the movement speed of the piston 30 when the filter element 40 is a filter element to be tested, m1 represents the air circulation rate per unit time of the standard filter element, and m2 represents the air circulation rate per unit time of the filter element to be tested.

[0084] It can be understood that since the above parameters such as V, n, Δt, S, v1, m1, m2, etc. are all known parameters, the movement speed v2 of the piston 30 adapted to the current filter element to be tested when the pipette performs suction and blowing can be obtained through the first proportional relationship formula and the second proportional relationship formula. On this basis, when the pipette performs suction or blowing, it is only necessary to let the piston 30 of the pipette move at the calculated movement speed within the Δt time, so that the air pressure change inside the first chamber 50 can be kept as consistent as possible when the filter element to be tested is not a standard filter element.

[0085] In some embodiments of the present application, the derivation process of the first proportional relationship is:

[0086] Assume that the air flow rate per unit time of the standard filter element is m1, and the air flow rate per unit time of the filter element to be tested is m2; then, within the time Δt, the amount of the substance in the air entering the first chamber 50 through the standard filter element is:

[0087] Δn1=m1Δt (3).

[0088] The amount of substance in the air entering the first chamber 50 through the filter element to be tested is:

[0089] Δn2=m2Δt (4).

[0090] It can be understood that when the pipette performs the suction action, the piston 30 will move in the direction away from the filter element 40, the volume of the first chamber 50 will increase, and the air outside the first chamber 50 will enter the first chamber 50 through the filter element 40. However, due to the presence of the filter element 40, the rate of change of the amount of air inside the first chamber 50 is much smaller than the rate of change of the volume of the first chamber 50. Therefore, according to the ideal gas state equation, in order to keep the air inside the first chamber 50 in an equilibrium state, the air pressure inside the first chamber 50 will have a downward trend. Therefore, it can be obtained:

[0091] (P-ΔP1)(V+ΔV1)=(n+Δn1)RT (5),

[0092] (P-ΔP2)(V+ΔV2)=(n+Δn2)RT (6),

[0093] In the above formulas (5) and (6), P represents the air pressure of the first chamber 50 in the initial state, R represents the molar gas constant, T is the temperature, ΔP1 represents the air pressure change value of the first chamber 50 within the time Δt when the filter element 40 is a standard filter element, ΔP2 represents the air pressure change value of the first chamber 50 within the time Δt when the filter element 40 is a filter element to be tested, ΔV1 represents the volume change value of the first chamber 50 within the time Δt when the filter element 40 is a standard filter element, and ΔV2 represents the volume change value of the first chamber 50 within the time Δt when the filter element 40 is a filter element to be tested.

[0094] Assuming the cross-sectional area of ​​the first chamber 50 is S, we have:

[0095] ΔV1=v1ΔtS (7),

[0096] ΔV2=v2ΔtS (8),

[0097] On this basis, in order to ensure that the air pressure change in the first chamber 50 remains as consistent as possible when the filter element to be tested is not a standard filter element and the pipette performs an aspiration action, let P-ΔP1=P-ΔP2, then:

[0098]

[0099] Substituting the above equations (3), (4), (7) and (8) into equation (9) respectively, we can obtain the first proportional relationship equation.

[0100] In some embodiments of the present application, the derivation process of the second proportional relationship is:

[0101] Assume that the air flow rate per unit time of the standard filter element is m1, and the air flow rate per unit time of the filter element to be tested is m2;

[0102] Then, during the time Δt, the amount of substance in the air flowing out of the first chamber 50 through the standard filter element is:

[0103] Δn1=m1Δt (10),

[0104] The amount of substance in the air flowing out of the first chamber 50 through the filter element to be tested is:

[0105] Δn2=m2Δt (11);

[0106] It can be understood that when the pipette performs the blowing action, the piston 30 will move toward the filter element 40, the volume of the first chamber 50 will decrease, and the air inside the first chamber 50 will flow out of the first chamber 50 through the filter element 40. However, due to the presence of the filter element 40, the rate of change of the amount of air inside the first chamber 50 is much smaller than the rate of change of the volume of the first chamber 50. Therefore, according to the ideal gas state equation, in order to keep the air inside the first chamber 50 in a balanced state, the air pressure inside the first chamber 50 will have an upward trend. Therefore, it can be obtained that:

[0107] (P+ΔP1)(V-ΔV1)=(n-Δn1)RT (12),

[0108] (P+ΔP2)(V-ΔV2)=(n-Δn2)RT (13),

[0109] In the above formulas (12) and (13), P represents the air pressure of the first chamber 50 in the initial state, R represents the molar gas constant, T represents the temperature, ΔP1 represents the air pressure change value of the first chamber 50 within Δt when the filter element 40 is a standard filter element, ΔP2 represents the air pressure change value of the first chamber 50 within Δt when the filter element 40 is a test filter element, ΔV1 represents the volume change value of the first chamber 50 within Δt when the filter element 40 is a standard filter element, and ΔV2 represents the volume change value of the first chamber 50 within Δt when the filter element 40 is a test filter element;

[0110] Assuming the cross-sectional area of ​​the first chamber 50 is S, we have:

[0111] ΔV1=v1ΔtS (14),

[0112] ΔV2=v2ΔtS (15),

[0113] On this basis, in order to ensure that the air pressure change in the first chamber 50 remains as consistent as possible when the filter element to be tested is not a standard filter element and the pipette performs the blowing action, let P+ΔP1=P+ΔP2, then:

[0114]

[0115] Substituting the above equations (10), (11), (14) and (15) into equation (16) respectively, we can obtain the second proportional relationship equation.

[0116] In order to more clearly and intuitively demonstrate the beneficial effects of the method provided in this application, this application also tests pipettes using filter elements 40 with different densities.

[0117] like Figures 2 to 4 As shown, Figure 2The figure shows the pressure waveform of the first chamber 50 when the pipette uses a standard filter element and performs suction and blowing actions. Figure 3 The figure shows the pressure waveform of the first chamber 50 when the pipette uses a filter element to be tested that is not a standard filter element and performs suction and blowing actions. Figure 4 The graph shows the pressure waveform of the first chamber 50 when the pipette uses a filter element to be tested that is not a standard filter element and corrects the movement speed of the piston 30 to perform suction and blowing actions.

[0118] Depend on Figure 2 and Figure 3 It can be seen that when the filter element to be tested is not a standard filter element and the movement speed of the piston 30 is not corrected, the air pressure change value of the first chamber 50 is about 6 times the air pressure change value of the first chamber 50 corresponding to the standard filter element. Figure 4 It can be seen that by correcting the movement speed of the pipette piston 30 when the filter element to be tested is not a standard filter element, the air pressure change value of the first chamber 50 corresponding to the filter element to be tested can be made equal to the air pressure change value of the first chamber 50 corresponding to the filter element to be tested. Figure 2 The air pressure change values ​​of the first chamber 50 corresponding to the standard filter element shown remain substantially consistent.

[0119] The above are merely preferred embodiments of the present application and are not intended to limit the present application. Those skilled in the art will readily appreciate that various modifications and variations are possible. Any modifications, equivalent substitutions, or improvements made within the spirit and principles of the present application shall be included within the scope of protection of the present application.

Claims

1. A method for improving the liquid level detection and liquid aspiration and discharge performance of a pipette, wherein the pipette comprises a pipette tip and a tip connected to the pipette tip, wherein a piston is provided inside the pipette tip, a filter is provided inside the tip tip, and a first chamber is formed between the piston and the filter, wherein: The filter element is divided into a standard filter element and a filter element to be tested, and the method comprises the following steps: S1. Obtaining the first pressure change data inside the first chamber when the filter element is the standard filter element, so as to use the first pressure change data as the standard pressure change data; S2 obtains the second pressure change data inside the first chamber when the filter element is the filter element to be tested; S3. Comparing the second pressure change data with the standard pressure change data to determine whether the filter element to be tested is the standard filter element. If the filter element to be tested is not the standard filter element, executing step S4; S4. Based on the proportional relationship between the standard filter element and the filter element to be tested, the piston movement speed that is compatible with the current filter element to be tested is calculated; In step S4, the proportional relationship between the standard filter element and the filter element to be tested includes a first proportional relationship and a second proportional relationship; The first proportional relationship is the proportional relationship between the standard filter element and the filter element to be tested when the pipette performs an aspiration action, and is expressed as: （1）， The second proportional relationship is the proportional relationship between the standard filter element and the filter element to be tested when the pipette performs the blowing action, and is expressed as: （2）， In the above formulas (1) and (2), V represents the volume of the first chamber in the initial state, n represents the amount of air in the first chamber in the initial state, Δt represents the movement time of the piston, S represents the cross-sectional area of ​​the first chamber, v1 represents the movement speed of the piston when the filter element is the standard filter element, v2 represents the movement speed of the piston when the filter element is the filter element to be tested, m1 represents the air flow rate per unit time of the standard filter element, and m2 represents the air flow rate per unit time of the filter element to be tested.

2. The method for improving the liquid level detection and liquid suction and discharge performance of a pipette according to claim 1, characterized in that: The derivation process of the first proportional relationship is: Assume that the air flow rate per unit time of the standard filter element is m1, and the air flow rate per unit time of the filter element to be tested is m2; Then, during the time Δt, the amount of the substance in the air entering the first chamber through the standard filter element is: （3）， The amount of substance in the air entering the first chamber through the filter element to be tested is: （4）； According to the ideal gas state equation, （5）， （6）， In the above formulas (5) and (6), P represents the air pressure of the first chamber in the initial state, R represents the molar gas constant, T represents the temperature, ΔP1 represents the air pressure change value of the first chamber within Δt when the filter element is the standard filter element, ΔP2 represents the air pressure change value of the first chamber within Δt when the filter element is the filter element to be tested, ΔV1 represents the volume change value of the first chamber within Δt when the filter element is the standard filter element, and ΔV2 represents the volume change value of the first chamber within Δt when the filter element is the filter element to be tested; Assuming the cross-sectional area of ​​the first chamber is S, we have: （7）， （8）， make , then: （9）， Substituting the above equations (3), (4), (7) and (8) into equation (9) respectively, we can obtain the first proportional relationship equation.

3. The method for improving the liquid level detection and liquid suction and discharge performance of a pipette according to claim 1, characterized in that: The derivation process of the second proportional relationship is: Assume that the air flow rate per unit time of the standard filter element is m1, and the air flow rate per unit time of the filter element to be tested is m2; Then, during the time Δt, the amount of substance in the air flowing out of the first chamber through the standard filter element is: （10）， The amount of substance in the air flowing out of the first chamber through the filter element to be tested is: （11）； According to the ideal gas state equation, （12）， （13）， In the above formulas (12) and (13), P represents the air pressure of the first chamber in the initial state, R represents the molar gas constant, T represents the temperature, ΔP1 represents the air pressure change value of the first chamber within Δt when the filter element is the standard filter element, ΔP2 represents the air pressure change value of the first chamber within Δt when the filter element is the filter element to be tested, ΔV1 represents the volume change value of the first chamber within Δt when the filter element is the standard filter element, and ΔV2 represents the volume change value of the first chamber within Δt when the filter element is the filter element to be tested; Assuming the cross-sectional area of ​​the first chamber is S, we have: （14）， （15）， make , then: （16）， Substituting the above equations (10), (11), (14) and (15) into equation (16) respectively, we can obtain the second proportional relationship.

4. The method for improving the liquid level detection and liquid suction and discharge performance of a pipette according to claim 1, characterized in that: In step S1, the first air pressure change data is obtained by the pipette performing an action of suctioning, blowing or piercing the TIP head.

5. The method for improving the liquid level detection and liquid suction and discharge performance of a pipette according to claim 1, characterized in that: In step S2, the second air pressure change data is obtained by the pipette performing an action of suctioning, blowing or piercing the TIP head.

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