Phytochemical rapid collection device and collection method

Abstract

The plant chemical type rapid collection device and collection method provided by the application, wherein the plant chemical type rapid collection device comprises a mounting seat, a pull rod and a sampling needle wrapped with stainless steel fibers, the pull rod is coaxially connected with the sampling needle and is arranged in the axial direction of the mounting seat, a sealing sleeve is arranged on the side end face of the mounting seat facing the sampling needle, and the sampling needle can be accommodated in the sealing sleeve; the stainless steel fibers are provided with a silica resin-PMSQ microsphere coating. The plant chemical type rapid collection device and collection method provided by the application adopt a new type of polymethylsilsesquioxane microsphere coating, so that the plant chemical type rapid collection device, especially the sampling needle, has the characteristics of high temperature resistance, good acid and alkali resistance and high extraction efficiency, and can fully absorb plant tissue components in a short time; the plant chemical type rapid collection device is designed and manufactured with a sealing sleeve, and can temporarily store plant samples for a long time.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of plant identification, in particular to a plant chemical type rapid collection device and collection method. BACKGROUND

[0002] Plant chemical type refers to the same plant with no obvious difference in morphology, but the chemical components contained are different, which can be divided into various types, and is a manifestation of plant intraspecific biodiversity. People usually identify the chemical type by observing the subtle differences in plant morphology, combined with the smell of branches, leaves and wood, which is commonly known as morphological identification method. This method requires experienced professionals, is time-consuming and laborious, and cannot meet the rapid and effective identification of large quantities of samples. In modern chromatography technology, gas chromatography-mass spectrometry is widely used, which is a very effective method for analyzing plant essential oil components, and can qualitatively and quantitatively analyze volatile oils with extremely complex chemical composition.

[0003] In order to identify the plant chemical type, essential oil needs to be extracted first, and then the essential oil components are analyzed. In the traditional method, the extraction of essential oil includes direct distillation extraction, steam distillation extraction and simultaneous distillation extraction methods, which are time-consuming and complicated. Through molecular marker technology, DNA analysis of different chemical type plants reveals their molecular genetic characteristics; using surface desorption atmospheric pressure chemical ionization mass spectrometry, different plant chemical types can be quickly analyzed according to the characteristic mass spectrum signals of plants, and no complex pretreatment process is required. The above methods all need to pick fresh leaves for in vitro analysis, and the sample pretreatment process is limited by the time and space of sampling, and the analysis results cannot reflect the metabolism of the leaves. It is of great significance to explore a kind of in vivo sampling analysis without complex sample pretreatment process and to realize the rapid identification of plant chemical type, which is of great significance to the rational development and utilization of plant resources, quality control of medicinal materials, and prevention and control of plant diseases and pests.

[0004] Solid phase microextraction technology plays an important role in the analysis of volatile and semi-volatile components. Compared with traditional solid phase microextraction devices, the new type of solid phase microextraction arrow coating has larger volume and better enrichment effect, but the types of solid phase microextraction arrow coating on the market are limited and the precision of the extraction results reported in the literature needs to be further improved. The sealing and adsorption properties of the equipment are weak, making it difficult to maintain the quality of the collected samples for a long time, and the reproducibility of the test is poor. SUMMARY

[0005] Therefore, it is necessary to provide a plant chemical type rapid collection device and collection method aiming at at least one of the above-mentioned problems.

[0006] The first aspect provides a plant chemical type rapid collection device, comprising a mounting seat, a pull rod and a sampling needle wrapped with stainless steel fibers, the pull rod is coaxially connected with the sampling needle and is arranged in the axial direction of the mounting seat, a sealing sleeve is arranged on the side end face of the mounting seat facing the sampling needle, and the sampling needle can be accommodated in the sealing sleeve.

[0007] The stainless steel fibers are coated with a coating of silica resin-PMSQ microspheres.

[0008] In some implementations of the first aspect, a first detachable nut is arranged on the pull rod, the first detachable nut is arranged at the top end of the pull rod, and the sampling needle is connected to the bottom end of the pull rod.

[0009] In combination with the first aspect and the above implementations, in some implementations of the first aspect, a sealing ring and a second detachable nut are arranged on the side end face of the mounting seat facing the pull rod, the second detachable nut is threadedly connected to the sealing ring, the sealing ring is connected to the mounting seat, and the pull rod passes through the sealing ring and the second detachable nut.

[0010] In combination with the first aspect and the above implementations, in some implementations of the first aspect, a detachable sealing gasket is further arranged between the sealing ring and the second detachable nut.

[0011] In combination with the first aspect and the above implementations, in some implementations of the first aspect, sealing plugs are arranged at both ends of the sampling needle, and the sealing plugs match the inner cavity of the sealing sleeve.

[0012] In combination with the first aspect and the above implementations, in some implementations of the first aspect, the preparation steps of the silica resin-PMSQ microsphere coating include:

[0013] Ammonia water is added to a 20% volume percentage methyltrimethoxysilane hydrolysis solution, the product is centrifuged after standing and reacting, the obtained product is washed with ethanol and water respectively, and the dried product is obtained.

[0014] Dichloromethane is used as a solvent, a coating solute is prepared in a proportion of silica resin: curing agent: PMSQ microspheres = 2:1:1, dichloromethane solvent is added to the coating solute, the coating solution is obtained after mixing, the sampling needle is inserted into the coating solution, and the sampling needle is repeatedly vertically lifted and pulled for several times, and then the sampling needle is aged at 300°C for half an hour.

[0015] In combination with the first aspect and the above implementations, in some implementations of the first aspect, the step of washing the obtained product with ethanol and water respectively includes:

[0016] The product is filtered, washed with 95% ethanol for three times, without drying, directly dispersed in 80% ethanol solution, and treated with 0.05 mol / L sodium hydroxide solution at 70°C for 10 h.

[0017] The second product is filtered to remove the solvent, washed with 95% ethanol and water alternately until the filtrate is neutral, and dried at 80°C under vacuum to obtain the mesoporous polymethylsilsesquioxane microspheres after alkaline heat treatment.

[0018] In a second aspect, the present application provides a plant chemical type rapid collection method, which uses the plant chemical type rapid collection device described in any one of the first aspect of the present application, and the steps include:

[0019] The pulling rod is pushed to fully extend the sampling needle out of the sealed sleeve, to puncture the plant, so that the stainless steel fibers on the sampling needle, which are coated with silicone-PMSQ microspheres, are in full contact with the plant tissue;

[0020] The pulling rod is pulled to fully retract the sampling needle into the sealed sleeve.

[0021] In some implementations of the second aspect, the step of puncturing the plant includes:

[0022] The leaf of the plant is grabbed, the leaf is bent several times, the sampling needle is punctured into the leaf, avoiding the leaf veins, and passing through the upper epidermis, palisade tissue, spongy tissue, protective cells and lower epidermis of the leaf.

[0023] In a third aspect, the present application provides a plant chemical type rapid collection method, which includes a drone and a plant chemical type rapid collection device described in any one of the first aspect of the present application, the plant chemical type rapid collection device is arranged on the drone, and the sampling needle extends out of the sealed sleeve; the drone is operated to the location of the plant to be tested, stays for several minutes, and then returns and stores the sampling needle into the sealed sleeve.

[0024] The technical solutions provided in the embodiments of the present application have the following beneficial technical effects:

[0025] The plant chemical type rapid collection device and collection method provided by the present application use a new type of polymethylsilsesquioxane microsphere coating, so that the plant chemical type rapid collection device, especially the sampling needle therein, has the characteristics of high temperature resistance, good acid and alkali resistance, and high extraction efficiency, and can fully absorb plant tissue components in a short time; the plant chemical type rapid collection device is designed and manufactured with a sealed sleeve, which can temporarily store plant samples for a long time; the plant volatile secondary metabolites are sampled in situ and in vivo by using the plant leaf puncture method and the unmanned aerial vehicle air sampling method, so that the plant chemical type can be rapidly identified.

[0026] Additional aspects and advantages of the application will be set forth in the description that follows, and in part will be obvious from the description, or can be learned by the practice of the application. BRIEF DESCRIPTION OF DRAWINGS

[0027] Figure 1 The schematic diagram of the planar structure of the plant chemical type rapid collection device in an embodiment of the application;

[0028] Figure 2 The comparative graph of the thermogravimetric analysis of the silicone-PMSQ microsphere coating in an embodiment of the application;

[0029] Figure 3 The schematic diagram of the preparation method flow of the silicone-PMSQ microsphere coating in an embodiment of the application;

[0030] Figure 4 The comparative schematic diagram of the extraction camphor peak of the self-made coating and the commercialized coating in an embodiment of the application;

[0031] Figure 5 The nitrogen adsorption-desorption curve and the pore size distribution curve of the PMSQ microsphere in an embodiment of the application;

[0032] Figure 6 The comparative schematic diagram of the extraction performance of the self-made microsphere and the commercialized microsphere in an embodiment of the application;

[0033] Figure 7 The schematic diagram of the specific step flow of the product washed by ethanol and water respectively in an embodiment of the application;

[0034] Figure 8 The schematic diagram of the flow of the plant chemical type rapid collection method in an embodiment of the application;

[0035] Figure 9 The graph of the solid phase microextraction of the Cinnamomum paniculatum leaf in the silicone-PMSQ microsphere coating in an embodiment of the application in the real-time puncture sampling gas chromatography combined with the identification of two kinds of camphor tree chemical types in the campus;

[0036] Figure 10 The graph of the unmanned aerial belt arrow-shaped solid phase microextraction component air sampling-gas chromatography combined with the identification of two kinds of camphor tree chemical types in the campus in an embodiment of the application.

[0037] BRIEF DESCRIPTION OF DRAWINGS

[0038] 100 - mounting seat, 200 - pull rod, 300 - sampling needle;

[0039] 110 - sealing sleeve, 120 - sealing ring, 130 - second detachable nut, 140 - detachable sealing gasket;

[0040] 210 - first detachable screw cap;

[0041] 310 - stainless steel fiber, 320 - sealing plug. DETAILED DESCRIPTION

[0042] For the purpose of promoting an understanding of the application, the application will now be described in greater detail with reference to the figures. The figures show possible embodiments of the application. However, the application can be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and fully convey the scope of the application to those skilled in the art. Additionally, it should be understood that the terminology used herein is for the purpose of describing particular embodiments only, and is not intended to be limiting.

[0043] As will be understood by one of skill in the relevant art, all terms used herein, including technical and scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs unless otherwise specifically defined. It will be further understood that terms, such as those defined in commonly used dictionaries, should be interpreted as having a meaning that is consistent with their meaning in the context of the relevant art and will not be interpreted in an idealized or overly formal sense unless expressly so defined herein.

[0044] As will be understood by one of skill in the relevant art, all terms used herein, including technical and scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs unless otherwise specifically defined. It will be further understood that terms, such as those defined in commonly used dictionaries, should be interpreted as having a meaning that is consistent with their meaning in the context of the relevant art and will not be interpreted in an idealized or overly formal sense unless expressly so defined herein.

[0045] The technical solutions of the present application and how the technical solutions solve the above technical problems will be described in detail below with specific embodiments.

[0046] Embodiments of the first aspect of the present application provide a plant chemical type rapid collection device, as shown in the drawings, comprising a mounting seat 100, a pull rod 200 and a sampling needle 300 wrapped with a stainless steel fiber 310, the pull rod 200 is coaxially connected with the sampling needle 300 and is arranged in the axial direction of the mounting seat 100, a sealing sleeve 110 is arranged on the side end face of the mounting seat 100 facing the sampling needle 300, and the sampling needle 300 can be accommodated in the sealing sleeve 110; Figure 1

[0047] ​The stainless steel fiber 310 is coated with a coating of silica resin-PMSQ microspheres.

[0048] The sampling needle 300 of the phytochemical type rapid collection device of the present application is coated with a stainless steel fiber 310, in particular, the stainless steel fiber 310 is coated with a coating of silica resin-PMSQ microspheres, PMSQ being polymethylsilsesquioxane, and the coating has excellent extraction performance, such as Figure 2 as shown, and the coating composition has a reasonable allocation ratio, effectively solving the problem of easy cracking of thick coating, longer service life, and lower comprehensive sampling cost.

[0049] Optionally, in some implementations of the first aspect of the present application, a first detachable nut 210 is arranged on the pull rod 200, the first detachable nut 210 is arranged at the top end of the pull rod 200, and the sampling needle 300 is connected to the bottom end of the pull rod 200.

[0050] Optionally, in some implementations of the first aspect of the present application, as shown in Figure 1 , a sealing ring 120 and a second detachable nut 130 are arranged on the side end face of the mounting seat 100 facing the pull rod 200, the second detachable nut 130 is threadedly connected to the sealing ring 120, the sealing ring 120 is connected to the mounting seat 100, and the pull rod 200 passes through the sealing ring 120 and the second detachable nut 130.

[0051] As shown in Figure 1 , in combination with the embodiments of the first aspect and the above implementations, in some implementations of the first aspect of the present application, a detachable sealing gasket 140 is further arranged between the sealing ring 120 and the second detachable nut 130. The sealing gasket 140 can solve the problem of gas leakage caused by the fixed sealing gasket used for a long time, which may lead to poor reproducibility of data results. Solid-phase microextraction devices can be divided into manual and automatic types. Commercial multifunctional full-automatic sample pretreatment platforms have a wide range of applications, but the platforms are expensive, and commercial manual type conventional SPME probes use quartz fiber as the substrate, which is not resistant to direct piercing sampling. Based on the re-design of the sealing gasket structure of the gas chromatography gasification chamber, a detachable arrow-shaped solid-phase microextraction sealing device is provided. For durable coatings, the sealing device can realize regular replacement of the sealing gasket, which can effectively improve the reliability of the analysis results.

[0052] Optionally, in combination with the embodiments of the first aspect and the above implementations, in some implementations of the first aspect of the present application, as shown in Figure 1 , sealing plugs 320 are arranged at both ends of the sampling needle 300, and the sealing plugs 320 are matched with the inner cavity of the sealing sleeve 110. By arranging the sealing plugs 320 at both ends, when the sampling needle 300 is stored in the sealing sleeve 110, a small sealing structure is formed by the sealing plugs 320, which increases the temporary storage period of the sample.

[0053] Optionally, in combination with the first aspect embodiment and the above implementation manners, in some other specific implementation manners of the first aspect of the present application, as shown in Figure 3 The preparation steps of the silicone-PMSQ microsphere coating include:

[0054] S10: Ammonia is added to the 20% volume percentage methyltrimethoxysilane hydrolysis solution, and the product is centrifuged after standing reaction. The obtained product is washed with ethanol and water respectively, and dried to obtain polymethylsilsesquioxane microspheres.

[0055] S20: Dichloromethane is used as the solvent, and the coating solute is prepared in a proportion of silicone: curing agent: PMSQ microsphere = 2:1:1. Dichloromethane solvent is added to the coating solute, and the coating solution is obtained after mixing. The sampling needle 300 is inserted into the coating solution, and repeatedly vertically pulled for several times. Then the sampling needle 300 is aged at 300℃ for half an hour.

[0056] As shown in Figures 4-6 , the self-made PMSQ microspheres treated by alkaline heat have larger specific surface area and pore size than the untreated commercial PMSQ microspheres. As shown in Figure 5 , the specific surface area is 420m 2 g -1 , and the average pore size is 6nm; which is more conducive to the adsorption of extraction target. As shown in Figure 6 , the untreated commercial PMSQ microspheres have large particle size but not obvious adsorption effect, while the self-made PMSQ microspheres have significant adsorption effect, even if the microsphere particle size is large, they still show the best extraction performance among the four.

[0057] In combination with the first aspect embodiment and the above implementation manners, in some implementation manners of the first aspect embodiment, as shown in Figure 7 The step of washing the obtained product with ethanol and water respectively includes:

[0058] S21: The product is filtered and washed with 95% ethanol for three times. Without drying, the product is directly dispersed in 80% ethanol solution, and treated by alkaline at 70℃ for 10h using 0.05mol·L-1 sodium hydroxide solution.

[0059] S22: The second product is obtained by filtering to remove the solvent. The second product is washed with 95% ethanol and water alternately until the filtrate is neutral. The mesoporous polymethylsilsesquioxane microspheres after alkaline heat treatment are obtained by drying at 80℃ under vacuum.

[0060] The specific high-temperature resistant silicone resin is selected as the adhesive, the vertical pulling method is used to prepare the coating under the optimized conditions, which can effectively improve the batch-to-batch reproducibility of the uniformity and extraction effect of the coating. The acid and alkali resistance and temperature resistance of the coating are effectively improved, and the number of coating uses is greater than 300 times.

[0061] Based on the same inventive concept, embodiments of the second aspect of the present application provide a method for rapid collection of plant chemistry, using the plant chemistry rapid collection device as described in any one of the first aspect of the present application, as shown in Figure 8 The specific steps include:

[0062] S1000: Push the pull rod 200 to fully extend the sampling needle 300 out of the sealed sleeve 110, puncture the plant, so that the stainless steel fiber 310 on the sampling needle 300 with a silicone-PMSQ microsphere coating is in full contact with the plant tissue.

[0063] S2000: Pull the pull rod 200 to fully retract the sampling needle 300 into the sealed sleeve 110.

[0064] When directly puncturing the plant leaf, remove the detachable screw cap, remove the hollow pull rod 200, insert the stainless steel arrow into the leaf, push out the coating, start extraction, and after the extraction is completed, retract the sampling needle 300, and assemble the extraction device. Insert the extraction probe, i.e. the sampling needle 300 into the sample inlet of the gas chromatograph, and pyrolyze at a high temperature of 250°C for 3 min. Then the extraction probe can be thermally desorbed at a high temperature of 280°C for 10 min before it can be extracted again.

[0065] Specifically, in some implementations of the second aspect, the step of puncturing the plant includes: grabbing the leaf of the plant, bending the leaf several times, puncturing the leaf with the sampling needle 300, avoiding the leaf veins on the leaf, and passing through the upper epidermis, palisade tissue, spongy tissue, guard cells and lower epidermis of the leaf.

[0066] When puncturing the leaf, the leaf veins are avoided as much as possible, and the needle puncture process mainly passes through the upper epidermis, palisade tissue, spongy tissue, guard cells and lower epidermis of the leaf surface. After puncturing, the cross section of the leaf exchanges substances with the surface of the solid phase microextraction coating, and the volatile components of the leaf can be sampled in situ, in vivo and in real time. The experiment optimizes the influence of puncture residence time on the chromatogram signal; bending the leaf before puncturing can effectively increase the contact area between the leaf and the solid phase microextraction coating, which is beneficial to the exchange of substances. The experiment optimizes the influence of the number of leaf bending times on the chromatogram signal; after in-situ sampling, the extraction coating is retracted into the sealed sleeve 110, and the sampling needle 300 in the device and the sealed sleeve 110 can produce a good sealing effect, which is beneficial to bringing the arrow-shaped solid phase microextraction assembly back to the laboratory for gas chromatography analysis. The experiment investigates the influence of different storage times on the chromatogram signal.

[0067] Thirdly, this application provides a method for rapid phytochemical sampling, including a drone and a rapid phytochemical sampling device as described in any of the first aspects of this application. The rapid phytochemical sampling device is mounted on the drone, and a sampling needle 300 extends out of the sealing sleeve 110. The drone is operated to the location of the plant to be tested, stays for several minutes, and then returns and retracts the sampling needle 300 into the sealing sleeve 110.

[0068] Unmanned aerial vehicles (UAVs) are flexible, convenient, and intelligently operable, and have made significant progress in on-site sampling and analysis of environmental pollutants in recent years. This experiment used a UAV carrying the rapid phytochemical sampling device provided in this application, or at least its solid-phase microextraction component, to sample and analyze the air surrounding camphor trees. The results were compared with those from on-site puncture sampling of a single leaf of the same plant, demonstrating its application in the rapid identification of camphor tree chemotypes under different growth conditions.

[0069] The following are specific examples:

[0070] Example 1

[0071] Silicone-PMSQ microsphere coated solid-phase microextraction of camphor tree leaves and in vivo puncture solid-phase microextraction-gas chromatography analysis for identification of the chemical type of camphor trees on campus.

[0072] 1. Optimization of in vivo puncture time

[0073] When puncturing the leaves, the veins should be avoided as much as possible. The puncture process mainly penetrates the upper epidermis, palisade tissue, spongy tissue, protective cells, and lower epidermis of the leaf surface. After puncture, the leaf cross-section exchanges substances with the surface of the solid-phase microextraction coating, enabling on-site, real-time, and efficient sampling of volatile components from the leaves. The experiment optimized the effect of puncture residence time on the chromatographic signal.

[0074] 2. Optimization of blade bending frequency

[0075] Bending the leaves before puncture can effectively increase the contact area between the leaves and the solid-phase microextraction coating, which is beneficial for substance exchange. Fresh camphor tree leaves were collected and used in the laboratory for leaf bending and puncture optimization experiments.

[0076] 3. Optimized preservation time after puncture

[0077] After on-site sampling, the sampling needle with the extraction coating was retracted into the sealing sleeve. The sealing plug and sealing sleeve in the device provide a good seal, facilitating the return of the arrow-shaped solid-phase microextraction assembly to the laboratory for gas chromatography analysis. The experiment investigated the effect of different storage times on the chromatographic signal. The experimental results are as follows: Figure 9 As shown.

[0078] Example 2

[0079] Silicone-PMSQ microsphere-coated solid phase microextraction device for sampling air around cinnamomum camphora by unmanned aerial vehicle, and identification of chemical types of cinnamomum camphora in campus by solid phase microextraction-gas chromatography analysis.

[0080] The sampling needle in the arrow-shaped solid phase microextraction device is pushed out, and the device is fixed on the unmanned aerial vehicle body, and the unmanned aerial vehicle is controlled to fly to a suitable height position around the cinnamomum camphora and hover for 10 hours to complete air sampling. After the unmanned aerial vehicle lands, the arrow-shaped solid phase microextraction device is removed, the sampling needle is recovered, and the sample is analyzed by gas chromatography-mass spectrometry. The test results are shown in Figure 10 .

[0081] The technical solutions provided in the embodiments of the present application have the following beneficial technical effects:

[0082] The plant chemical type rapid collection device and the collection method provided by the present application adopt a new type of polymethylsilsesquioxane microsphere coating, so that the plant chemical type rapid collection device, especially the sampling needle therein, has the characteristics of high temperature resistance, good acid and alkali resistance, and high extraction efficiency, and can fully absorb plant tissue components in a short time; the plant chemical type rapid collection device is designed and manufactured with a sealing sleeve, so that the plant sample can be temporarily stored for a long time; the plant volatile secondary metabolites are sampled in situ on the living body by using the plant leaf in vivo direct puncture method and the unmanned aerial vehicle air sampling method, so that the plant chemical type can be rapidly identified.

[0083] Those skilled in the art can understand that the steps, measures and schemes in various operations, methods and processes discussed in the present application can be alternated, changed, combined or deleted. Further, other steps, measures and schemes in various operations, methods and processes discussed in the present application can also be alternated, changed, rearranged, decomposed, combined or deleted. Further, the steps, measures and schemes in various operations, methods and processes in the prior art can also be alternated, changed, rearranged, decomposed, combined or deleted.

[0084] The terms "first", "second" are only used for description purposes, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of indicated technical features. Therefore, the features defined with "first", "second" can explicitly or implicitly include one or more features. In the description of the present application, unless otherwise specified, the meaning of "a plurality of" is two or more.

[0085] In the description of the present application, it should be noted that unless specifically stated and limited otherwise, the terms "mounting", "connected", "connecting" or "linking" should be interpreted broadly, for example, can be fixed connection, can also be detachable connection, or integral connection; can be directly connected, can also be indirectly connected through an intermediate medium, can be internal communication of two elements. For those skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0086] In the description of the present application, specific features, structures, materials or characteristics can be combined in any one or more embodiments or examples in a suitable manner.

[0087] It should be understood that although each step in the flowchart of the accompanying drawings is displayed in sequence according to the direction of the arrow, these steps are not necessarily executed in sequence according to the direction of the arrow. Unless otherwise specified herein, the execution of these steps is not strictly limited in sequence, and they can be executed in other orders. Moreover, at least part of the steps in the flowchart of the accompanying drawings can include a plurality of sub-steps or stages, which are not necessarily executed at the same time, but can be executed at different times, and the execution sequence is not necessarily sequential, but can be alternately or alternately executed with at least part of other steps or other steps, sub-steps or stages.

[0088] The above is only part of the embodiments of the present application, and it should be pointed out that for those skilled in the art, without departing from the principles of the present application, a number of improvements and refinements can be made, which should also be regarded as the protection scope of the present application.

Claims

1. A rapid phytochemical sampling device, characterized in that, The device includes a mounting base, a pull rod, and a sampling needle with a surface covered with stainless steel fibers. The pull rod is coaxially connected to the sampling needle and passes through the axial direction of the mounting base. A sealing sleeve is provided on the end face of the mounting base facing the sampling needle. The sampling needle can be housed in the sealing sleeve. Both ends of the sampling needle are provided with sealing plugs, and the sealing plugs match the inner cavity of the sealing sleeve. The stainless steel fiber is coated with a silicone resin-polymethylsilsesquioxane microsphere coating, and the preparation steps of the silicone resin-polymethylsilsesquioxane microsphere coating include: Ammonia was added to a 20% (v / v) methyltrimethoxysilane hydrolysate. After standing and reacting, the product was centrifuged and filtered. The product was washed three times with 95% ethanol and dispersed directly in an 80% ethanol solution without drying. It was then alkali-heated at 70°C for 10 hours with a 0.05 mol / L sodium hydroxide solution. The solvent was removed by filtration to obtain a second product. The second product was washed alternately with 95% ethanol and water until the filtrate was neutral. It was then vacuum-dried at 80°C to obtain alkali-heated mesoporous polymethylsilsesquioxane microspheres. A coating solute was prepared using dichloromethane as a solvent and a ratio of silicone resin:curing agent:polymethylsilsesquioxane microspheres = 2:1:

1. Dichloromethane solvent was added to the coating solute and mixed to obtain a coating solution. The sampling needle was inserted into the coating solution and repeatedly pulled vertically several times. The sampling needle was then aged at 300°C for half an hour.

2. The phytochemical rapid sampling device according to claim 1, characterized in that, The pull rod is provided with a first detachable nut, which is located at the top of the pull rod, and the sampling needle is connected to the bottom of the pull rod.

3. The phytochemical rapid sampling device according to claim 1, characterized in that, A sealing ring and a second removable nut are provided on the end face of the mounting base facing the pull rod. The second removable nut is threaded onto the sealing ring, and the sealing ring is connected to the mounting base. The pull rod passes through the sealing ring and the second removable nut.

4. The rapid phytochemical sampling device according to claim 3, characterized in that, A removable sealing gasket is also provided between the sealing ring and the second removable nut.

5. A rapid method for collecting phytochemical samples, characterized in that, The steps of using the phytochemical rapid collection device as described in any one of claims 1 to 4 include: Pushing the lever will fully extend the sampling needle out of the sealing sleeve, piercing the plant, so that the stainless steel fiber on the sampling needle, which is coated with silicone resin-polymethylsilsesquioxane microspheres, comes into full contact with the plant tissue. Pull the lever to retract the sampling needle completely into the sealing sleeve.

6. The rapid collection method for phytochemical types according to claim 5, characterized in that, The steps of piercing the plant include: Grasp a plant leaf, bend the leaf several times, and pierce the leaf with the sampling needle, avoiding the veins on the leaf, and pass through the upper epidermis, palisade tissue, spongy tissue, protective cells and lower epidermis of the leaf.

7. A rapid phytochemical sampling method, characterized in that, The device includes a drone and a phytochemical rapid sampling device as described in any one of claims 1 to 4, wherein the phytochemical rapid sampling device is mounted on the drone and the sampling needle extends out of the sealing sleeve; the drone is operated to the location of the plant to be tested, stays for several minutes, returns, and retracts the sampling needle into the sealing sleeve.

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