Method for preparing liquid printed matter based on polysaccharide and amino polydimethylsiloxane and application thereof

Abstract

The present invention relates to a method for preparing liquid printed matter based on polysaccharide and amino polydimethylsiloxane, and the method comprises: step 1, preparing a polysaccharide solution with deionized water as solvent, stirring at room temperature for 2 hours, and then putting it in a refrigerator for 8-12 hours; step 2, dissolving an additive in the polysaccharide solution to prepare an additive-polysaccharide working solution; step 3, preparing an NH2-PDMS-NH2 solution with soybean oil as solvent and stirring for 2 hours to fully dissolve, thereby obtaining an NH2-PDMS-NH2 solution; step 4, placing the additive-polysaccharide working solution in a syringe, immersing it in the NH2-PDMS-NH2 solution, and injecting the additive-polysaccharide working solution in the syringe at a fixed speed to obtain a spherical structure liquid printed matter loaded with additives or a continuous filamentous structure liquid printed matter. The present invention uses NH2-PDMS-NH2 as a fat-soluble nucleophilic reagent to easily capture H + Anionic polysaccharides carry a positive charge while negatively charged in aqueous solution, which enables encapsulation and shaping of the liquid, increases the stability of the system, and serves as a delivery vehicle for water-soluble nutritional factors.

Description

Technical Field

[0001] The invention belongs to the technical field of new materials, and specifically relates to a method for preparing liquid printed matter based on polysaccharide and amino polydimethylsiloxane and application thereof. Background Art

[0002] Although some water-soluble bioactive substances such as tea polyphenols, anthocyanins, ascorbic acid, pyridoxine, pantothenic acid and biotin are easily metabolized and absorbed, their biological activity is easily destroyed by light and heat in solution. Therefore, effective encapsulation can greatly improve their bioavailability.

[0003] At present, water-soluble substances can be encapsulated in the form of water-in-water or oil-in-water emulsions. However, the preparation of such emulsions requires high energy consumption, such as high-speed shearing, high-pressure homogenization or ultrasonic dispersion; moreover, water-in-water or oil-in-water emulsion systems are very easy to become unstable, and are prone to demulsification and phase inversion during preparation and storage; furthermore, the current industrial chains in the fields of medicine, health food and food all require a liquid printing method with a stable system and easy operation and customized shape.

[0004] Therefore, in view of the above technical problems, it is necessary to provide a method and application of preparing liquid printed matter based on polysaccharide and amino polydimethylsiloxane. Summary of the invention

[0005] In view of the above problems, the present invention provides a method and application for preparing liquid printed matter based on polysaccharide and amino polydimethylsiloxane (hereinafter referred to as NH2-PDMS-NH2). The present invention uses NH2-PDMS-NH2 as a fat-soluble nucleophilic reagent to easily capture H + The positive charge and negative charge of some anionic polysaccharides in aqueous solution induce the polysaccharides and NH2-PDMS-NH2 to form cluster complexes at the oil / water interface under electrostatic interaction, and evolve into a membrane-like interface layer, thereby achieving encapsulation and shaping of the internal liquid and increasing the stability of the system. This method has low energy consumption and is easy to operate, and can be used as a delivery carrier for water-soluble nutritional factors.

[0006] In order to achieve the above object, the present invention adopts the following technical solutions.

[0007] A method for preparing a liquid printed material based on polysaccharide and amino polydimethylsiloxane, the specific steps comprising:

[0008] Step 1: First, prepare a polysaccharide solution with deionized water as solvent, stir it magnetically at room temperature for 2 hours, and then put it in a refrigerator and store it at 4°C for 8-12 hours to achieve sufficient hydration;

[0009] Step 2, then, prepare NH2-PDMS-NH2 solution with soybean oil as solvent and stir magnetically for 2 hours to fully dissolve, to obtain NH2-PDMS-NH2 solution for standby use;

[0010] Step 3. Finally, the polysaccharide solution is placed in a syringe, immersed in the NH2-PDMS-NH2 solution, and the polysaccharide solution is injected at a fixed speed to obtain a spherical structure liquid print or a continuous filament structure liquid print.

[0011] Furthermore, if a spherical structure liquid print is obtained, the concentration of the polysaccharide solution in step 1 is 0.25%-0.75%; if a continuous filamentary structure liquid print is obtained, the concentration of the polysaccharide solution in step 1 is 0.50%-2.00%.

[0012] Furthermore, in step 1, the polysaccharide is any one of soybean seed coat polysaccharide, pectin, xanthan gum, gum arabic, carrageenan, hyaluronic acid, fucoidan, carboxymethyl cellulose, sodium carboxymethyl cellulose, sodium alginate or β-glucan.

[0013] Furthermore, if a spherical structure liquid print is obtained, the concentration of the NH2-PDMS-NH2 solution in step 2 includes 0.10%-0.50%; if a continuous filamentary structure liquid print is obtained, the concentration of the NH2-PDMS-NH2 solution in step 2 includes 1.00%-2.00%.

[0014] Furthermore, if a spherical structure liquid print is obtained, the injection speed of step 3 is 1 μL / s; if a continuous filamentary structure liquid print is obtained, the injection speed of step 3 is 2 μL / s, and the needle movement trajectory needs to be controlled.

[0015] The present invention also provides an application of preparing liquid printed matter based on polysaccharide and amino polydimethylsiloxane, that is, loading water-soluble functional factors.

[0016] An application of preparing liquid printed matter based on polysaccharide and amino polydimethylsiloxane, preparing liquid printed matter loaded with additives, the specific steps are:

[0017] (1) Step 1, prepare a polysaccharide solution with deionized water as solvent, stir magnetically at room temperature for 2 hours, and then place in a refrigerator and store at 4°C for 8-12 hours;

[0018] (2) Step 2, dissolving the additive in the polysaccharide solution to prepare an additive-polysaccharide working solution of a certain concentration;

[0019] (3) Step 3, using soybean oil as solvent to prepare NH2-PDMS-NH2 solution and magnetically stirring for 2 h to fully dissolve, to obtain NH2-PDMS-NH2 solution for later use;

[0020] (4) Step 4, the additive-polysaccharide working solution is placed in a syringe, immersed in the NH2-PDMS-NH2 solution, and then the additive-polysaccharide working solution in the syringe is injected at a fixed speed to obtain a spherical structure liquid print loaded with the additive or a continuous filamentary structure liquid print.

[0021] Furthermore, the concentration of the additive in the additive-polysaccharide working solution in step 2 is 0.10%-1.50%.

[0022] Furthermore, if a spherical structure liquid print loaded with additives is obtained, the concentration of the polysaccharide solution in step 1 includes 0.10%-0.50%; if a continuous filamentary structure liquid print loaded with additives is obtained, the concentration of the polysaccharide solution in step 1 includes 0.50%-2.00%.

[0023] Furthermore, if a spherical structure liquid print loaded with additives is obtained, the concentration of the NH2-PDMS-NH2 solution in step 3 includes 0.10%-0.50%; if a continuous filamentary structure liquid print loaded with additives is obtained, the concentration of the NH2-PDMS-NH2 solution in step 3 includes 1.00%-2.00%.

[0024] Furthermore, if a spherical structure liquid print loaded with additives is obtained, the injection speed of step 4 is 1 μL / s; if a continuous filamentary structure liquid print loaded with additives is obtained, the injection speed of step 3 is 2 μL / s, and the needle movement trajectory needs to be controlled.

[0025] Furthermore, in step 2, the additive is any one of tea polyphenols, anthocyanidins, ascorbic acid, pyridoxine, pantothenic acid, biotin, prebiotic factors or probiotics.

[0026] Furthermore, in step 1, the concentration of the polysaccharide solution is 0.25%-2.00%.

[0027] Further, in step 3, the concentration of the NH2-PDMS-NH2 solution is 0.10%-2.00%.

[0028] Advantages and effects of the present invention

[0029] (1) The present invention utilizes the principle of electrostatic complexation between polysaccharides and NH2-PDMS-NH2 at the oil / water interface to prepare spherical structure printed materials or continuous filamentary structure liquid printed materials by injection, which is easy to operate and has low energy consumption;

[0030] (2) The liquid printed material prepared by the present invention has good stability, and the water-soluble functional factors encapsulated inside are not easy to leak, thereby improving the bioavailability of the functional factors;

[0031] (3) The liquid printed material prepared by the present invention has clear and complete patterns, and the product form is novel and can be used for large-scale industrial production. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1 The formation process of the spherical structure printing in Example 3;

[0033] Figure 2 This is a scanning electron microscope image of the interior of the interface film extracted in Example 4;

[0034] Figure 3 is the isothermal titration curve of soybean seed coat polysaccharide and amino-terminated polydimethylsiloxane under the concentration conditions of Example 4;

[0035] Figure 4 The infrared spectra of the interface films produced in Examples 1, 2 and 4;

[0036] Figure 5 The formation process and morphology of the printed filamentary structure in Example 5;

[0037] Figure 6 The formation process and morphology of the printed filamentary structure in Example 6;

[0038] Figure 7 The target morphology of the printed material of the filamentous structure loaded with tea polyphenols prepared under the conditions of Example 9 is "luck"

[0039] The word "happy" and a jellyfish pattern. DETAILED DESCRIPTION

[0040] The specific implementation of the present invention is described in detail below. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0041] Example 1

[0042] Preparation of liquid printed objects with spherical structures

[0043] (1) First, a 0.50% soybean seed coat polysaccharide (hereinafter referred to as SHP) solution was prepared with deionized water as the solvent, magnetically stirred at room temperature for 2 h, and then placed in a refrigerator and stored at 4°C for 8 h to allow for sufficient hydration;

[0044] (2) Then, a 0.10% NH2-PDMS-NH2 solution was prepared using soybean oil as solvent, and magnetic stirring was performed for 2 h to fully dissolve the solution for later use;

[0045] (3) Finally, the SHP solution was placed in a syringe and immersed in the NH2-PDMS-NH2 solution. The SHP solution was injected at a fixed speed of 1 μL / s to obtain a spherical structure liquid print.

[0046] Example 2

[0047] Preparation of liquid printed objects with spherical structures

[0048] (1) First, a 0.50% SHP solution was prepared with deionized water as the solvent, magnetically stirred at room temperature for 2 h, and then placed in a refrigerator and stored at 4 °C for 8 h to allow for sufficient hydration;

[0049] (2) Then, 0.50% NH2-PDMS-NH2 solution was prepared using soybean oil as solvent, and magnetic stirring was performed for 2 h to fully dissolve the solution for later use;

[0050] (3) Finally, the SHP solution was placed in a syringe, immersed in the NH2-PDMS-NH2 solution, and the SHP solution was injected at a fixed speed of 1 μL / s to obtain a spherical structure liquid print.

[0051] Example 3

[0052] Preparation of liquid printed objects with spherical structures

[0053] (1) First, a 0.75% SHP solution was prepared with deionized water as the solvent, magnetically stirred at room temperature for 2 h, and then placed in a refrigerator and stored at 4 °C for 10 h to allow for sufficient hydration;

[0054] (2) Then, 0.50% NH2-PDMS-NH2 solution was prepared using soybean oil as solvent, and magnetic stirring was performed for 2 h to fully dissolve the solution for later use;

[0055] (3) Finally, the SHP solution was placed in a syringe, immersed in the NH2-PDMS-NH2 solution, and the SHP solution was injected at a fixed speed of 1 μL / s to obtain a discontinuous spherical structure liquid print.

[0056] like Figure 1 As shown in the formation process of the spherical structure liquid print in Example 3, it can be seen that the descending liquid flow will break into individual droplets during the injection process. In addition, a stable interface film is formed on the surface of the droplet so that the droplets will not coalesce.

[0057] Example 4

[0058] Preparation of continuous filamentary structure liquid printed objects

[0059] (1) First, a 0.50% SHP solution was prepared with deionized water as the solvent, magnetically stirred at room temperature for 2 h, and then placed in a refrigerator and stored at 4 °C for 12 h to allow for sufficient hydration;

[0060] (2) Then, a 1.00% NH2-PDMS-NH2 solution was prepared using soybean oil as solvent, and magnetic stirring was performed for 2 h to fully dissolve the solution for later use;

[0061] (3) Finally, the SHP solution was placed in a syringe, immersed in the NH2-PDMS-NH2 solution, and the SHP solution was injected at a fixed speed of 2 μL / s to obtain a continuous filamentous structure liquid print.

[0062] The interface film of Example 4 was subjected to a scanning electron microscope test. Figure 2 As shown, the interface layer shows a closely arranged, uniformly sized spherical structure, the surface of the sphere is full of protrusions, and the sphere is directly covered with a film and filament. Therefore, it is preliminarily speculated that SHP and NH2-PDMS-NH2 undergo an electrostatic complexation reaction to reach the lowest energy state - spherical, and some NH2-PDMS-NH2 can serve as a bridge to connect the spheres to form a cluster, and then the branched structure of the polysaccharide connects the clusters to form a coherent film system.

[0063] In addition, isothermal titration and infrared experiments were also performed in this example. Figure 3 The isothermal titration experiment results of titrating SHP solution with NH2-PDMS-NH2 solution, and ignoring the thermal reaction generated by background titration, the thermal reaction curve can meet the formula fitting of multi-site reaction type, which is consistent with the large number of reaction sites (free carboxyl groups) carried on the polysaccharide chain. In addition, it can be obtained by calculation that ΔH=132.89kJ / mol, TΔS=208.62kJ / mol, ΔG=-75.70kJ / mol, indicating that the reaction is a spontaneous reaction dominated by entropy, with the characteristics of electrostatic reaction. In addition, the reaction stoichiometric numbers n1 and n2 are approximately 8 and 4, indicating that each SHP undergoes complexation reaction with approximately 12 NH2-PDMS-NH2. Figure 4 A and B are respectively SHP, NH2-PDMS-NH2 and Example 4, and the infrared spectra of the interface films produced by Examples 1, 2 and 4 are compared. Figure 4 In A, it can be seen that the characteristic peak of SHP (1745cm -1 ,-COOMe) becomes weaker, which is due to the decrease in the proportion of SHP under the same sample mass, while 1640cm -1 (-COO -) did not maintain a similar downward trend, but rather red-shifted, indicating that NH2-PDMS-NH2 caused the dissociation of the SHP carboxyl group, leading to a complex reaction between the two. Figure 4 The characteristic peaks of NH2-PDMS-NH2 and SHP in B (corresponding to the right and left bands in the figure, respectively) remain almost unchanged, which means that after changing the concentration ratio of the two, the two will still maintain a consistent reaction ratio. Combined with the ITC results, it can be considered that the reaction ratio of SHP and NH2-PDMS-NH2 is always 1:12. However, SHP at 3500cm -1 The characteristic peak area representing -OH that appears on the left and right decreases more significantly with the increase of NH2-PDMS-NH2 content, which once again shows that NH2-PDMS-NH2 promotes the dissociation of hydrogen ions on the non-free carboxyl groups on SHP to promote a more complete combination of the two, thereby achieving the formation of a complete and dense composite film between SHP and NH2-PDMS-NH2 at the oil-water interface to prevent the leakage of the internal liquid, allowing the liquid printing process to proceed successfully.

[0064] Example 5

[0065] Preparation of continuous filamentary structure liquid printed objects

[0066] (1) First, a 0.50% SHP solution was prepared with deionized water as the solvent, magnetically stirred at room temperature for 2 h, and then placed in a refrigerator and stored at 4 °C for 10 h to allow for sufficient hydration;

[0067] (2) Then, a 2.00% NH2-PDMS-NH2 solution was prepared using soybean oil as solvent, and magnetic stirring was performed for 2 h to fully dissolve the solution for later use;

[0068] (3) Finally, the SHP solution was placed in a syringe, immersed in the NH2-PDMS-NH2 solution, and the SHP solution was injected at a fixed speed of 2 μL / s to obtain a continuous filamentous structure liquid print.

[0069] like Figure 5 As shown, the formation process of the filamentary structure liquid printed object in Example 5 can be seen as compared with Example 3 ( Figure 1 ), after the concentration of NH2-PDMS-NH2 is greatly increased, the descending liquid flow presents a coherent shape. This is because compared with low concentration, NH2-PDMS-NH2 under high concentration conditions can form more clustered complexes with SHP, resulting in a more coherent, complete and thicker interface film. The diameter of the liquid flow is different from top to bottom and presents a gourd shape, which can meet the needs of liquid printing with special textures.

[0070] Example 6

[0071] Preparation of continuous filamentary structure liquid printed objects

[0072] (1) First, a 1.00% SHP solution was prepared with deionized water as the solvent, magnetically stirred at room temperature for 2 h, and then placed in a refrigerator and stored at 4 °C for 12 h to allow for sufficient hydration;

[0073] (2) Then, a 2.00% NH2-PDMS-NH2 solution was prepared using soybean oil as solvent, and magnetic stirring was performed for 2 h to fully dissolve the solution for later use;

[0074] (3) Finally, the SHP solution was placed in a syringe, immersed in the NH2-PDMS-NH2 solution, and the SHP solution was injected at a fixed speed of 2 μL / s to obtain a continuous filamentous structure liquid print.

[0075] like Figure 6 As shown, the formation process of the continuous filamentous carrier in Example 6 can be seen compared with Examples 5 and Figure 5 , Figure 6 The descending liquid flow in the middle is coherent and has consistent diameters from top to bottom, which further indicates that due to the increase in SHP concentration, the number of clustered complexes formed during the injection process increases, resulting in enhanced thickness of the interface layer and improved uniformity and continuity, which can be widely used in liquid printing.

[0076] Example 7

[0077] Preparation of continuous filamentous structure liquid printed materials loaded with tea polyphenols

[0078] (1) First, a 0.50% SHP solution was prepared with deionized water as the solvent, magnetically stirred at room temperature for 2 h, and then placed in a refrigerator and stored at 4 °C for 8 h to allow for sufficient hydration;

[0079] (2) Then, tea polyphenols were dissolved in the SHP solution to prepare a tea polyphenol-SHP working solution with a concentration of 1.00%;

[0080] (3) Then, a 1.50% NH2-PDMS-NH2 solution was prepared using soybean oil as solvent, and magnetic stirring was performed for 2 h to fully dissolve the solution for later use;

[0081] (4) Finally, the tea polyphenol-SHP working solution was placed in a syringe, immersed in the NH2-PDMS-NH2 solution, and the tea polyphenol-SHP working solution was injected at a fixed speed of 2 μL / s to obtain a continuous filamentous structure liquid print.

[0082] Example 8

[0083] Preparation of continuous filamentous structure liquid printed materials loaded with tea polyphenols

[0084] (1) First, a 0.75% SHP solution was prepared with deionized water as the solvent, magnetically stirred at room temperature for 2 h, and then placed in a refrigerator and stored at 4 °C for 10 h to allow for sufficient hydration;

[0085] (2) Then, tea polyphenols were dissolved in the SHP solution to prepare a tea polyphenol-SHP working solution with a concentration of 1.00%;

[0086] (3) Then, a 1.50% NH2-PDMS-NH2 solution was prepared using soybean oil as solvent, and magnetic stirring was performed for 2 h to fully dissolve the solution for later use;

[0087] (4) Finally, the tea polyphenol-SHP working solution was placed in a syringe, immersed in the NH2-PDMS-NH2 solution, and the tea polyphenol-SHP working solution was injected at a fixed speed of 2 μL / s to obtain a continuous filamentous structure liquid print.

[0088] By using the method for preparing liquid printed matter based on polysaccharide and amino polydimethylsiloxane of this embodiment, a spherical structure liquid printed matter or a continuous filament structure liquid printed matter can be obtained, and then a printed matter of a specific shape can be produced by controlling the needle trajectory, thereby obtaining a specific pattern. The specific embodiments are as follows:

[0089] Example 9

[0090] Preparation of liquid printed materials with specific patterns loaded with tea polyphenols

[0091] (1) First, a 1.00% SHP solution was prepared with deionized water as the solvent, magnetically stirred at room temperature for 2 h, and then placed in a refrigerator and stored at 4 °C for 12 h to allow for sufficient hydration;

[0092] (2) Then, tea polyphenols were dissolved in the SHP solution to prepare a tea polyphenol-SHP working solution with a concentration of 1.00%;

[0093] (3) Then, a 1.50% NH2-PDMS-NH2 solution was prepared using soybean oil as solvent, and magnetic stirring was performed for 2 h to fully dissolve the solution for later use;

[0094] (4) Finally, the tea polyphenol-SHP working solution is placed in a syringe, immersed in the NH2-PDMS-NH2 solution, and the tea polyphenol-SHP working solution is injected at a fixed speed of 2 μL / s. By controlling the trajectory of the syringe, a liquid print with a specific pattern loaded with tea polyphenols can be obtained.

[0095] like Figure 7As shown, the filamentous printed material with a specific pattern loaded with tea polyphenols prepared under the conditions of Example 9 demonstrated good shaping ability through the brown appearance of tea polyphenols. The words "luck" and "happy" as well as the pattern of jellyfish were clearly visible. The surface was smooth within 5 days. Although there were wrinkles on the surface after 7 days, the overall shape was complete and there was no leakage of the contents, indicating that this liquid printing method is highly feasible.

[0096] Example 10

[0097] Preparation of liquid printed materials with specific patterns loaded with tea polyphenols

[0098] (1) First, a 1.00% pectin solution was prepared with deionized water as the solvent, magnetically stirred at room temperature for 2 h, and then placed in a refrigerator and stored at 4°C for 12 h to allow for sufficient hydration;

[0099] (2) Then, the tea polyphenols are dissolved in the pectin solution to prepare a tea polyphenol-pectin working solution with a concentration of 1.00%;

[0100] (3) Then, a 1.50% NH2-PDMS-NH2 solution was prepared using soybean oil as solvent, and magnetic stirring was performed for 2 h to fully dissolve the solution for later use;

[0101] (4) Finally, the tea polyphenol-pectin working solution is placed in a syringe, immersed in the NH2-PDMS-NH2 solution, and the tea polyphenol-pectin working solution is injected at a fixed speed of 2 μL / s. By controlling the trajectory of the syringe, a liquid print with a specific pattern loaded with tea polyphenols can be obtained.

[0102] Embodiment 11

[0103] Preparation of liquid printed materials with specific patterns loaded with tea polyphenols

[0104] (1) First, a 1.00% xanthan gum solution was prepared with deionized water as the solvent, magnetically stirred at room temperature for 2 h, and then placed in a refrigerator and stored at 4°C for 12 h to allow for sufficient hydration;

[0105] (2) Then, tea polyphenols were dissolved in the xanthan gum solution to prepare a tea polyphenol-xanthan gum working solution with a concentration of 1.00%;

[0106] (3) Then, a 1.50% NH2-PDMS-NH2 solution was prepared using soybean oil as solvent, and magnetic stirring was performed for 2 h to fully dissolve the solution for later use;

[0107] (4) Finally, the tea polyphenol-xanthan gum working solution is placed in a syringe, immersed in the NH2-PDMS-NH2 solution, and the tea polyphenol-xanthan gum working solution is injected at a fixed speed of 2 μL / s. By controlling the trajectory of the syringe, a liquid print with a specific pattern loaded with tea polyphenols can be obtained.

[0108] Example 12

[0109] Preparation of liquid printed materials with specific patterns loaded with tea polyphenols

[0110] (1) First, a 1.00% gum arabic solution was prepared with deionized water as the solvent, magnetically stirred at room temperature for 2 h, and then placed in a refrigerator and stored at 4 °C for 12 h to allow for sufficient hydration;

[0111] (2) Then, the tea polyphenols were dissolved in the gum arabic solution to prepare a tea polyphenol-gum arabic working solution with a concentration of 1.00%;

[0112] (3) Then, a 1.50% NH2-PDMS-NH2 solution was prepared using soybean oil as solvent, and magnetic stirring was performed for 2 h to fully dissolve the solution for later use;

[0113] (4) Finally, the tea polyphenol-gum arabic working solution is placed in a syringe, immersed in the NH2-PDMS-NH2 solution, and the tea polyphenol-gum arabic working solution is injected at a fixed speed of 2 μL / s. By controlling the trajectory of the syringe, a liquid print with a specific pattern loaded with tea polyphenols can be obtained.

[0114] Example 13

[0115] Preparation of liquid printed materials with specific patterns loaded with tea polyphenols

[0116] (1) First, a 1.00% carrageenan solution was prepared with deionized water as the solvent, magnetically stirred at room temperature for 2 h, and then placed in a refrigerator and stored at 4°C for 12 h to allow for sufficient hydration;

[0117] (2) Then, tea polyphenols were dissolved in the carrageenan solution to prepare a carrageenan-SHP working solution with a concentration of 1.00%;

[0118] (3) Then, a 1.50% NH2-PDMS-NH2 solution was prepared using soybean oil as solvent, and magnetic stirring was performed for 2 h to fully dissolve the solution for later use;

[0119] (4) Finally, the tea polyphenol-carrageenan working solution is placed in a syringe, immersed in the NH2-PDMS-NH2 solution, and the tea polyphenol-carrageenan working solution is injected at a fixed speed of 2 μL / s. By controlling the trajectory of the syringe, a liquid print with a specific pattern loaded with tea polyphenols can be obtained.

[0120] Embodiment 14

[0121] Preparation of liquid printed materials with specific patterns loaded with tea polyphenols

[0122] (1) First, a 1.00% hyaluronic acid solution was prepared with deionized water as the solvent, magnetically stirred at room temperature for 2 h, and then placed in a refrigerator and stored at 4°C for 12 h to allow for adequate hydration;

[0123] (2) Then, the tea polyphenols are dissolved in the hyaluronic acid solution to prepare a tea polyphenol-hyaluronic acid working solution with a concentration of 1.00%;

[0124] (3) Then, a 1.50% NH2-PDMS-NH2 solution was prepared using soybean oil as solvent, and magnetic stirring was performed for 2 h to fully dissolve the solution for later use;

[0125] (4) Finally, the tea polyphenol-hyaluronic acid working solution is placed in a syringe, immersed in the NH2-PDMS-NH2 solution, and the tea polyphenol-hyaluronic acid working solution is injected at a fixed speed of 2 μL / s. By controlling the trajectory of the syringe, a liquid print with a specific pattern loaded with tea polyphenols can be obtained.

[0126] Embodiment 15

[0127] Preparation of liquid printed materials with specific patterns loaded with tea polyphenols

[0128] (1) First, a 1.00% sodium carboxymethyl cellulose solution was prepared with deionized water as the solvent, magnetically stirred at room temperature for 2 h, and then placed in a refrigerator and stored at 4°C for 12 h to allow for sufficient hydration;

[0129] (2) Then, the tea polyphenols are dissolved in a sodium carboxymethyl cellulose solution to prepare a tea polyphenol-sodium carboxymethyl cellulose working solution with a concentration of 1.00%;

[0130] (3) Then, a 1.50% NH2-PDMS-NH2 solution was prepared using soybean oil as solvent, and magnetic stirring was performed for 2 h to fully dissolve the solution for later use;

[0131] (4) Finally, the tea polyphenol-sodium carboxymethyl cellulose working solution is placed in a syringe, immersed in the NH2-PDMS-NH2 solution, and the tea polyphenol-sodium carboxymethyl cellulose working solution is injected at a fixed speed of 2 μL / s. By controlling the trajectory of the syringe, a liquid print with a specific pattern loaded with tea polyphenols can be obtained.

[0132] Example 16

[0133] Preparation of liquid printed materials with specific patterns loaded with tea polyphenols

[0134] (1) First, a 1.00% sodium alginate solution was prepared with deionized water as the solvent, magnetically stirred at room temperature for 2 h, and then placed in a refrigerator and stored at 4°C for 12 h to allow for sufficient hydration;

[0135] (2) Then, the tea polyphenols are dissolved in the sodium alginate solution to prepare a tea polyphenol-sodium alginate working solution with a concentration of 1.00%;

[0136] (3) Then, a 1.50% NH2-PDMS-NH2 solution was prepared using soybean oil as solvent, and magnetic stirring was performed for 2 h to fully dissolve the solution for later use;

[0137] (4) Finally, the tea polyphenol-sodium alginate working solution is placed in a syringe, immersed in the NH2-PDMS-NH2 solution, and the tea polyphenol-sodium alginate working solution is injected at a fixed speed of 2 μL / s. By controlling the trajectory of the syringe, a liquid print with a specific pattern loaded with tea polyphenols can be obtained.

[0138] Embodiment 17

[0139] Preparation of liquid printed materials with specific patterns loaded with anthocyanins

[0140] (1) First, a 1.00% SHP solution was prepared with deionized water as the solvent, magnetically stirred at room temperature for 2 h, and then placed in a refrigerator and stored at 4 °C for 12 h to allow for sufficient hydration;

[0141] (2) Then, anthocyanidin was dissolved in SHP solution to prepare an anthocyanidin-SHP working solution with a concentration of 1.00%;

[0142] (3) Then, a 1.50% NH2-PDMS-NH2 solution was prepared using soybean oil as solvent, and magnetic stirring was performed for 2 h to fully dissolve the solution for later use;

[0143] (4) Finally, the anthocyanin-SHP working solution was placed in a syringe, immersed in the NH2-PDMS-NH2 solution, and the anthocyanin-SHP working solution was injected at a fixed speed of 2 μL / s. By controlling the trajectory of the syringe, a liquid print with a specific pattern of anthocyanin loaded was obtained.

[0144] Embodiment 18

[0145] Preparation of liquid prints with specific patterns loaded with ascorbic acid

[0146] (1) First, a 1.00% SHP solution was prepared with deionized water as the solvent, magnetically stirred at room temperature for 2 h, and then placed in a refrigerator and stored at 4 °C for 12 h to allow for sufficient hydration;

[0147] (2) Then, ascorbic acid was dissolved in the SHP solution to prepare an ascorbic acid-SHP working solution with a concentration of 1.00%;

[0148] (3) Then, a 1.50% NH2-PDMS-NH2 solution was prepared using soybean oil as solvent, and magnetic stirring was performed for 2 h to fully dissolve the solution for later use;

[0149] (4) Finally, the ascorbic acid-SHP working solution is placed in a syringe, immersed in the NH2-PDMS-NH2 solution, and the ascorbic acid-SHP working solution is injected at a fixed speed of 2 μL / s. By controlling the trajectory of the syringe, a liquid print with a specific pattern loaded with ascorbic acid can be obtained.

[0150] Embodiment 19

[0151] Preparation of liquid prints with specific patterns loaded with pantothenic acid

[0152] (1) First, a 1.00% SHP solution was prepared with deionized water as the solvent, magnetically stirred at room temperature for 2 h, and then placed in a refrigerator and stored at 4 °C for 12 h to allow for sufficient hydration;

[0153] (2) Then, pantothenic acid was dissolved in the SHP solution to prepare a pantothenic acid-SHP working solution with a concentration of 1.00%;

[0154] (3) Then, a 1.50% NH2-PDMS-NH2 solution was prepared using soybean oil as solvent, and magnetic stirring was performed for 2 h to fully dissolve the solution for later use;

[0155] (4) Finally, the pantothenic acid-SHP working solution is placed in a syringe and immersed in the NH2-PDMS-NH2 solution. The pantothenic acid-SHP working solution is injected at a fixed speed of 2 μL / s, and a liquid print with a specific pattern of pantothenic acid can be obtained by controlling the trajectory of the syringe.

[0156] Embodiment 20

[0157] Preparation of liquid prints loaded with specific patterns of probiotic factors

[0158] (1) First, a 1.00% SHP solution was prepared with deionized water as the solvent, magnetically stirred at room temperature for 2 h, and then placed in a refrigerator and stored at 4 °C for 12 h to allow for sufficient hydration;

[0159] (2) Then, the probiotic factor is dissolved in the SHP solution to prepare a probiotic factor-SHP working solution with a concentration of 1.00%;

[0160] (3) Then, a 1.50% NH2-PDMS-NH2 solution was prepared using soybean oil as solvent, and magnetic stirring was performed for 2 h to fully dissolve the solution for later use;

[0161] (4) Finally, the probiotic factor-SHP working solution is placed in a syringe, immersed in the NH2-PDMS-NH2 solution, and the probiotic factor-SHP working solution is injected at a fixed speed of 2 μL / s. By controlling the trajectory of the syringe, a liquid print with a specific pattern of probiotic factors can be obtained.

[0162] Embodiment 21

[0163] Preparation of liquid prints with specific patterns loaded with probiotics

[0164] (1) First, a 1.00% SHP solution was prepared with deionized water as the solvent, magnetically stirred at room temperature for 2 h, and then placed in a refrigerator and stored at 4 °C for 12 h to allow for sufficient hydration;

[0165] (2) Then, the probiotics were dissolved in the SHP solution to prepare a probiotic-SHP working solution with a concentration of 1.00%;

[0166] (3) Then, a 1.50% NH2-PDMS-NH2 solution was prepared using soybean oil as solvent, and magnetic stirring was performed for 2 h to fully dissolve the solution for later use;

[0167] (4) Finally, the probiotic-SHP working solution was placed in a syringe, immersed in the NH2-PDMS-NH2 solution, and the probiotic-SHP working solution was injected at a fixed speed of 2 μL / s. By controlling the trajectory of the syringe, a liquid print with a specific pattern of probiotics loaded could be obtained.

[0168] The polysaccharide in this embodiment may also be some other anionic polysaccharides, and the additive may also be some other water-soluble bioactive substances, which are not listed one by one here.

[0169] The above are only specific embodiments of the present invention and are not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A method for preparing a liquid printed material based on polysaccharide and amino polydimethylsiloxane, characterized in that: The preparation method comprises: Step 1, preparing a polysaccharide solution with deionized water as solvent, stirring magnetically at room temperature, and storing at 4°C for 8-12 h; Step 2, preparing NH2-PDMS-NH2 solution with soybean oil as solvent, stirring magnetically until fully dissolved, and obtaining NH2-PDMS-NH2 solution for standby use; Step 3, the polysaccharide solution is placed in a syringe, immersed in the NH2-PDMS-NH2 solution, and the polysaccharide solution is injected at a fixed speed to obtain a spherical structure liquid print or a continuous filament structure liquid print; The concentration of the polysaccharide solution is 0.25%-2.00%; The concentration of the NH2-PDMS-NH2 solution is 0.10%-2.00%.

2. The method for preparing liquid printed matter based on polysaccharide and amino polydimethylsiloxane according to claim 1, characterized in that: In step 1, the polysaccharide is any one of soybean seed coat polysaccharide, pectin, xanthan gum, gum arabic, carrageenan, hyaluronic acid, phycocyanin, carboxymethyl cellulose, sodium carboxymethyl cellulose, sodium alginate or β-glucan.

3. The method for preparing liquid printed matter based on polysaccharide and amino polydimethylsiloxane according to claim 1, characterized in that: In step 3, the injection speed is 1 μL / s or 2 μL / s.

4. Application of a method for preparing liquid printed matter based on polysaccharide and amino polydimethylsiloxane, characterized in that: Specifically include: Step 1: Prepare the polysaccharide solution with deionized water as solvent, stir magnetically at room temperature for 2 h, and store at 4 °C for 8-12 h; Step 2: Dissolve the additive in the polysaccharide solution to prepare an additive-polysaccharide working solution; Step 3: Prepare NH2-PDMS-NH2 solution using soybean oil as solvent; Step 4: The additive-polysaccharide working solution is placed in a syringe, immersed in the NH2-PDMS-NH2 solution, and the additive-polysaccharide working solution is injected at a fixed speed to obtain a spherical structure liquid print loaded with the additive or a continuous filamentary structure liquid print; The concentration of the polysaccharide solution is 0.25%-2.00%; The concentration of the NH2-PDMS-NH2 solution is 0.10%-2.00%; The concentration of the additive in the additive-polysaccharide working solution is 0.10%-1.50%; The additive is any one of tea polyphenols, anthocyanidins, ascorbic acid, pyridoxine, pantothenic acid, biotin, prebiotic factors or probiotics.

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