Cucumber seed oil extract, methods of making and using same, cosmetics, pharmaceuticals

Abstract

The application discloses a cucumber seed oil extract, a preparation method and application thereof, cosmetics and medicines. The method comprises the following steps: providing dried cucumber seeds; seed oil extraction, comprising squeezing the dried cucumber seeds or performing supercritical carbon dioxide extraction after crushing to obtain crude cucumber seed oil; seed oil purification, comprising removing water-soluble organic acids in the crude cucumber seed oil to obtain an oil phase liquid; deodorization, comprising deodorizing the purified oil phase liquid to obtain deodorized crude cucumber seed oil; degreasing, comprising removing solid esters in the deodorized crude cucumber seed oil to obtain degreased crude cucumber seed oil; and impurity removal and drying of the degreased crude cucumber seed oil to obtain the cucumber seed oil extract. The preparation method of the cucumber seed oil extract is capable of obtaining the cucumber seed oil extract through drying, seed oil extraction, seed oil purification, deodorization, degreasing, impurity removal and drying in sequence, and has the advantages of simple operation, high yield, low cost, stable product quality, green and environment-friendly process and suitability for large-scale production.

Description

Technical Field

[0001] This application belongs to the technical field of seed oil extraction and application, and particularly relates to a cucumber seed oil extract, its preparation method and uses, cosmetics, and drugs. Background Art

[0002] Cucumber ( Cucumis sativus Linn.), also known as green cucumber, belongs to the Cucurbitaceae family. Cucumber was brought back to the Central Plains by Zhang Qian during his mission to the Western Regions in the Western Han Dynasty, so it was also called Hu cucumber. After the Later Zhao Emperor Shi Le in the period of the Five Hus and Sixteen Kingdoms disliked the word "Hu", Fan Tan, the governor of Xiangguo, a Han official, changed it to "cucumber". Cucumber is widely distributed throughout China and is one of the main greenhouse products.

[0003] Up to now, the research on cucumbers has mainly focused on food processing of their fruits, etc., and less research has been done on their seeds. Summary of the Invention

[0004] The embodiments of this application provide a cucumber seed oil extract, which can effectively promote the proliferation of immortalized human keratinocytes, reduce the production of inflammatory factors, have a strong anti-inflammatory effect, and at the same time can promote the expression of filaggrin and tight junction protein genes, having the effect of barrier repair.

[0005] In a first aspect, this application provides a preparation method of a cucumber seed oil extract, including:

[0006] Providing dried cucumber seeds;

[0007] Seed oil extraction, including pressing the dried cucumber seeds or performing supercritical carbon dioxide extraction after pulverization to obtain crude cucumber seed oil;

[0008] Seed oil purification, including removing water-soluble organic acids from the crude cucumber seed oil to obtain an oil-phase liquid;

[0009] Deodorization, including deodorizing the purified oil-phase liquid to obtain deodorized crude cucumber seed oil;

[0010] Degreasing, including removing solid esters from the deodorized crude cucumber seed oil to obtain degreased crude cucumber seed oil;

[0011] Removing impurities and drying the degreased crude cucumber seed oil to obtain the cucumber seed oil extract.

[0012] According to one aspect of this application, providing dried cucumber seeds includes:

[0013] Removing impurities other than cucumber seeds from the cucumber seeds,

[0014] Washing the cucumber seeds after removing impurities until the washing liquid becomes clear and transparent,

[0015] The washed cucumber seeds are dried to obtain dried cucumber seeds.

[0016] According to one aspect of this application, the temperature of the water used to wash the cucumber seeds after removing impurities until the washing liquid is clear and transparent is controlled at 20℃~50℃, optionally 25℃~35℃.

[0017] According to one aspect of this application, the washed cucumber seeds can be dried by outdoor air drying, vacuum drying, forced-air drying, or freeze drying.

[0018] According to one aspect of this application, the washed cucumber seeds may be dried using a forced-air drying method.

[0019] According to one aspect of this application, the seed oil extraction employs cold pressing extraction, comprising:

[0020] Dried cucumber seeds are pressed at 40℃~70℃ to obtain crude cucumber seed oil.

[0021] According to one aspect of this application, seed oil extraction is performed using an oil press for cold pressing.

[0022] According to one aspect of this application, the seed oil extraction employs supercritical CO2 extraction, comprising:

[0023] The dried cucumber seeds were crushed and sieved to obtain cucumber seed powder with a particle size of 10 to 50 mesh.

[0024] Cucumber seed powder was subjected to supercritical CO2 extraction to obtain crude cucumber seed oil.

[0025] According to one aspect of this application, the process parameters for supercritical CO2 extraction of seed oil meet the following requirements:

[0026] The supercritical CO2 extraction of cucumber seed powder was performed at a pressure of 25 MPa to 50 MPa, a flow rate of 25 L / h to 45 L / h, and a temperature of 30℃ to 55℃. o C, extraction time is 90min~180min.

[0027] According to one aspect of this application, seed oil purification includes:

[0028] Cucumber seed crude oil and purified water were mixed at a volume ratio of 1:2 to 5, and the mixture was heated to 70℃ to 80℃ for dispersion and allowed to stand to obtain an oil-water two-phase liquid.

[0029] The aqueous phase in the oil-water two-phase liquid is removed to obtain the oil phase liquid.

[0030] According to one aspect of this application, seed oil purification includes:

[0031] Cucumber crude seed oil and purified water were mixed at a volume ratio of 1:2 to 5, and the mixture was heated to 70℃ to 80℃ and dispersed at 100 rpm to 200 rpm for 2 to 3 hours. After standing for 10 to 12 hours, an oil-water two-phase liquid was obtained.

[0032] The aqueous phase containing water-soluble organic acids is removed from the oil-water two-phase liquid to obtain the oil phase liquid.

[0033] According to one aspect of this application, deodorization includes heating the oil phase liquid to 210°C. o C ~230 o C. Stir at 100rpm~200rpm for 2~3 hours to deodorize, then cool to obtain deodorized cucumber seed crude oil.

[0034] According to one aspect of this application, defatting includes maintaining the deodorized cucumber seed crude oil at a temperature of 2-5 degrees Celsius. o C. After 3-5 days, take the supernatant as defatted cucumber seed crude oil.

[0035] According to one aspect of this application, the process of removing impurities and drying defatted cucumber seed crude oil includes:

[0036] The defatted cucumber seed crude oil was filtered once, and then 0.1 wt.% to 0.5 wt.% of a desiccant was added to the filtrate for dispersion, followed by a second filtration to obtain cucumber seed oil extract.

[0037] According to one aspect of this application, the process of removing impurities and drying defatted cucumber seed crude oil includes:

[0038] The defatted cucumber seed crude oil was filtered once using a 0.1μm~0.8μm filter membrane. Then, 0.1wt.%~0.5wt.% of a chemical drying agent was added to the filtrate. The mixture was then dispersed at 100rpm~200rpm for 30min~60min. Finally, the dried product was filtered a second time using a 0.1μm~0.8μm filter membrane to obtain cucumber seed oil extract with a yield of 15%~18%.

[0039] According to one aspect of this application, the chemical desiccant is selected from anhydrous magnesium sulfate, anhydrous sodium sulfate, anhydrous calcium chloride, quicklime, or a combination thereof.

[0040] Secondly, this application provides a cucumber seed oil extract, which is prepared according to the above-described method for preparing cucumber seed oil extract.

[0041] According to one aspect of this application, the cucumber seed oil extract comprises, by weight percentage:

[0042] Linoleic acid 73%–76%, palmitic acid 10%–12%, oleic acid 8%–9%, ​​stearic acid 2%–3%, linolenic acid 2%–3%.

[0043] Thirdly, this application provides the use of cucumber seed oil extract in the preparation of anti-inflammatory drugs or cosmetics that repair cell barriers.

[0044] Fourthly, this application provides a cosmetic product comprising: the cucumber seed oil described above or a cucumber seed oil extract prepared according to the method for preparing cucumber seed oil.

[0045] According to one aspect of this application, the cosmetic also includes at least one of the following additives: wetting agent, moisturizer, thickener, emulsifier, emollient, surfactant, antioxidant, stabilizer, and preservative, and the cucumber seed oil extract accounts for 1 wt.% to 5 wt.% of the total mass of the cosmetic.

[0046] Fifthly, embodiments of this application provide a medicine comprising: the cucumber seed oil extract described above or a cucumber seed oil extract prepared according to the method for preparing cucumber seed oil extract.

[0047] In some optional embodiments of this application, the drug further includes at least one adjuvant selected from adjuvants, carriers, excipients, retention aids, sweeteners, diluents, preservatives, dyes / colorants, flavor enhancers, surfactants, wetting agents, dispersants, suspending agents, stabilizers, isotonic agents, solvents, or emulsifiers, and the cucumber seed oil extract accounts for 1 wt.% to 5 wt.% of the total mass of the drug.

[0048] The method for preparing cucumber seed oil extract according to the embodiments of this application involves sequentially drying, seed oil extraction, seed oil purification, deodorization, defatting, impurity removal, and drying to obtain cucumber seed oil extract. The preparation method is simple to operate, has a high yield and low cost, stable product quality, and is a green and environmentally friendly process, making it suitable for large-scale production.

[0049] The cucumber seed oil extract of this application embodiment can effectively promote the proliferation of immortalized human keratinocytes and reduce the production of inflammatory factors, thus having a strong anti-inflammatory effect. At the same time, it can also promote the expression of filaggrin and tight junction protein genes, thus having a barrier repair effect. Attached Figure Description

[0050] To more clearly illustrate the technical solutions of the embodiments of this application, the accompanying drawings used in the embodiments of this application will be briefly introduced below. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0051] Figure 1 This is a schematic flowchart of the preparation method of cucumber seed oil extract provided in the embodiments of this application;

[0052] Figure 2This is a comparison graph showing the effect of cucumber seed oil extract on the toxic stimulation test results of immortalized human keratinocytes compared with other control groups;

[0053] Figure 3 This is a comparison chart showing the effect of cucumber seed oil extract on the scratch repair effect of other control groups on immortalized human keratinocytes;

[0054] Figure 4 This is a comparison of the effects of cucumber seed oil extract and other control groups on NO release from mouse macrophages.

[0055] Figure 5 This is a comparison chart of the effects of cucumber seed oil extract and other control groups on TNF-α release from mouse macrophages;

[0056] Figure 6 This is a comparison diagram of the effects of cucumber seed oil extract and other control groups on the expression level of FLG protein in immortalized human keratinocytes.

[0057] Figure 7 This is a comparison diagram of the effects of cucumber seed oil extract and other control groups on the expression level of ZO-1 protein in immortalized human keratinocytes. Detailed Implementation

[0058] The features and exemplary embodiments of various aspects of this application will be described in detail below. To make the objectives, technical solutions, and advantages of this application clearer, the application will be further described in detail below with reference to the accompanying drawings and specific embodiments. It should be understood that the specific embodiments described herein are only intended to explain this application and not to limit it. For those skilled in the art, this application can be implemented without some of these specific details. The following description of the embodiments is merely to provide a better understanding of this application by illustrating examples.

[0059] It should be noted that, in this document, relational terms such as "first" and "second" are used merely to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Without further limitations, an element defined by the phrase "comprising..." does not exclude the presence of additional identical elements in the process, method, article, or apparatus that includes said element.

[0060] Cucumbers are one of the world's top ten vegetable crops and hold an irreplaceable and important position in China's fruit and vegetable market. Modern pharmacological research shows that the malonic acid in cucumbers can prevent the conversion of carbohydrates into fat, thus achieving the effects of weight loss, lowering blood pressure, and lowering blood lipids. Cucumber juice contains a large amount of water-soluble vitamins, which can beautify the skin, prevent pigmentation, and reduce wrinkles.

[0061] As described in the background section, existing research on cucumbers mainly focuses on the food processing of their fruit. Research on their seeds is relatively limited.

[0062] Cucumber seeds are the dried, mature seeds of cucumbers. According to the "Chinese Materia Medica," cucumber seeds have a long history of use in traditional medicine for treating diseases with significant effects, including promoting bone healing, supplementing calcium and strengthening bones, dispelling wind, and resolving phlegm. Cucumber seeds are rich in various amino acids, active peptides, proteins, and minerals, which can effectively maintain human bone metabolism, regulate calcium and phosphorus metabolism and bone volume, and have the effect of accumulating bone growth factors and inducing their secretion.

[0063] In order to make full use of abundant resources and to further explore new active natural products, this application conducts an in-depth and systematic study on the bioactivity of cucumber seed oil, and successfully studies the preparation method of cucumber seed oil through multiple experiments, and discovers new uses for cucumber seed oil extract.

[0064] To address the problems in the prior art, this application provides a cucumber seed oil extract, its preparation method and uses, as well as cosmetics and pharmaceuticals.

[0065] The preparation method of cucumber seed oil extract provided in the embodiments of this application will be described below first. Figure 1 A schematic flowchart of a method for preparing cucumber seed oil extract according to an embodiment of this application is shown. Figure 1 As shown, the preparation method of cucumber seed oil extract includes:

[0066] S1. Provides dried cucumber seeds;

[0067] S2. Seed oil extraction, including pressing dried cucumber seeds or crushing them and then extracting them with supercritical carbon dioxide to obtain crude cucumber seed oil.

[0068] S3. Seed oil purification, including removing water-soluble organic acids from crude cucumber seed oil to obtain an oil phase liquid;

[0069] S4. Deodorization, which includes deodorizing the purified oil phase liquid to obtain deodorized cucumber seed crude oil;

[0070] S5. Degreasing, including removing the solid fats from the deodorized cucumber seed crude oil to obtain defatted cucumber seed crude oil;

[0071] S6. Remove impurities from defatted cucumber seed crude oil and dry it to obtain cucumber seed oil extract.

[0072] The method for preparing cucumber seed oil extract according to the present application involves sequentially drying, seed oil extraction, seed oil purification, defatting, deodorization, impurity removal, and drying to obtain cucumber seed oil extract. The preparation method has the advantages of simple operation, high yield and low cost, stable product quality, and the process is green and environmentally friendly, making it suitable for large-scale production.

[0073] In some optional embodiments of this application, the dried cucumber seeds provided include:

[0074] Remove impurities other than the cucumber seed kernel from the cucumber seeds.

[0075] Wash the cucumber seeds after removing impurities until the washing solution is clear and transparent.

[0076] The washed cucumber seeds are dried to obtain dried cucumber seeds.

[0077] In the embodiments of this application, the cucumber seeds can be either existing cucumber seeds containing fatty acids or fresh cucumber seeds. During the impurity removal process, small stones, sand, plant roots, leaves, and other impurities are removed. The cucumber seeds after impurity removal are washed until the washing liquid is clear and transparent, indicating that the surface of the cucumber seeds no longer contains sand or other external impurities that would affect the preparation of cucumber seed oil extract.

[0078] In some optional embodiments of this application, the temperature of the water used to wash the cucumber seeds after impurity removal until the washing solution is clear and transparent is controlled at 20°C to 50°C, and optionally at 25°C to 35°C.

[0079] In some optional embodiments of this application, the washed cucumber seeds can be dried by outdoor air drying, vacuum drying, forced-air drying, or freeze drying.

[0080] In one embodiment, the cucumber seeds are preferably dried using a forced-air drying method.

[0081] In some optional embodiments of this application, seed oil extraction employs cold pressing extraction, including:

[0082] Dried cucumber seeds are pressed at 40℃~70℃ to obtain crude cucumber seed oil.

[0083] In some optional embodiments of this application, seed oil extraction is performed by cold pressing using an oil press.

[0084] The method for preparing cucumber seed oil extract according to the embodiments of this application can obtain crude cucumber seed oil with sediment after pressing by cold pressing.

[0085] In some optional embodiments of this application, cucumber seed oil extraction employs supercritical carbon dioxide extraction, including:

[0086] The dried cucumber seeds were crushed and sieved to obtain cucumber seed powder with a particle size of 10 to 50 mesh.

[0087] Cucumber seed powder was subjected to supercritical CO2 extraction to obtain crude cucumber seed oil.

[0088] The method for preparing cucumber seed oil extract according to the embodiments of this application can obtain slightly turbid crude cucumber seed oil by supercritical CO2 extraction.

[0089] In some optional embodiments of this application, the process parameters for supercritical carbon dioxide extraction of seed oil satisfy the following:

[0090] The extraction pressure for supercritical CO2 extraction of cucumber seed powder was 25 MPa to 50 MPa, the flow rate was 25 L / h to 45 L / h, and the temperature was 30℃ to 55℃. o C, extraction time is 90min~180min.

[0091] In the embodiments of this application, cucumber seed oil can also be extracted using organic solvent extraction.

[0092] In some optional embodiments of this application, seed oil purification includes:

[0093] Cucumber crude seed oil and purified water were mixed at a volume ratio of 1:2 to 5, and the mixture was heated to 70℃ to 80℃ for dispersion and allowed to stand to obtain an oil-water two-phase liquid.

[0094] The aqueous phase in the oil-water two-phase liquid is removed to obtain the oil phase liquid.

[0095] In some optional embodiments of this application, seed oil purification includes:

[0096] Cucumber crude seed oil and purified water were mixed at a volume ratio of 1:2 to 5, and the mixture was heated to 70℃ to 80℃ and dispersed at 100 rpm to 200 rpm for 2 to 3 hours. After standing for 10 to 12 hours, an oil-water two-phase liquid was obtained.

[0097] The aqueous phase containing water-soluble organic acids is removed from the oil-water two-phase liquid to obtain the oil phase liquid.

[0098] In the embodiments of this application, mixing cucumber seed crude oil with purified water can remove water-soluble organic acid components from the cucumber seed crude oil, reduce the acid value of the cucumber seed oil, and extend the shelf life of the cucumber seed oil.

[0099] In some optional embodiments of this application, deodorization includes:

[0100] Deodorization involves heating the oil phase liquid to 210°C. o C~230 o C. Stir at 100rpm~200rpm for 2~3 hours to deodorize, then cool to obtain deodorized cucumber seed crude oil.

[0101] In the embodiments of this application, removing the odor components from cucumber seed oil, increasing the smoke point of the oil, improving the flavor of the oil, and also improving the stability, color and quality of the oil.

[0102] In some optional embodiments of this application, degreasing includes:

[0103] Maintain the temperature of deodorized cucumber seed crude oil at 2-5 degrees Celsius. o C. After 3-5 days, take the supernatant as defatted cucumber seed crude oil.

[0104] In the embodiments of this application, solid fats in cucumber seed oil are removed to reduce the content of saturated fatty acids, prevent cucumber seed oil from solidifying and becoming cloudy at low temperatures, and maintain the transparency of cucumber seed oil.

[0105] In some optional embodiments of this application, the purification and drying of defatted cucumber seed crude oil includes:

[0106] The defatted cucumber seed crude oil was filtered once, and then 0.1 wt.% to 0.5 wt.% of a chemical drying agent was added to the filtrate for dispersion, followed by a second filtration to obtain cucumber seed oil extract.

[0107] In some optional embodiments of this application, filtration includes first filtering defatted cucumber seed crude oil using a 0.1 μm to 0.8 μm filter membrane, then adding 0.1 wt.% to 0.5 wt.% of a chemical drying agent to the filtrate, followed by dispersion at 100 rpm to 200 rpm for 30 min to 60 min, and then second filtering the dried product using a 0.1 μm to 0.8 μm filter membrane to obtain a yellow cucumber seed oil extract with a yield of 15% to 18%.

[0108] In some optional embodiments of this application, the chemical desiccant is selected from anhydrous magnesium sulfate, anhydrous sodium sulfate, anhydrous calcium chloride, quicklime, or a combination thereof.

[0109] In some optional embodiments of this application, filtration can be performed using any of the following methods: plate and frame filtration, pressure filtration, and vacuum filtration.

[0110] On the other hand, this application provides a cucumber seed oil extract prepared according to the above-described method for preparing cucumber seed oil extract.

[0111] In some optional embodiments of this application, the cucumber seed oil extract, by weight percentage, comprises:

[0112] The fatty acid composition of cucumber seed oil extract is 73%–76%, palmitic acid 10%–12%, oleic acid 8%–9%, ​​stearic acid 2%–3%, and linolenic acid 2%–3%. The detection method for fatty acid components in cucumber seed oil extract is in accordance with GB5009.168-2016.

[0113] The cucumber seed oil extract of this application embodiment can effectively promote the proliferation of immortalized human keratinocytes and reduce the production of inflammatory factors, thus having a strong anti-inflammatory effect. At the same time, it can also promote the expression of filaggrin and tight junction protein genes, thus having a barrier repair effect.

[0114] The cucumber seed oil extract of this application can be used in pharmaceuticals or cosmetics.

[0115] Thirdly, this application provides the use of cucumber seed oil extract in the preparation of anti-inflammatory and cell barrier repair drugs or cosmetics.

[0116] Fourthly, this application provides a cosmetic product comprising the above-mentioned cucumber seed oil extract or a cucumber seed oil extract prepared according to the preparation method of cucumber seed oil extract.

[0117] In some optional embodiments of this application, the cosmetic also includes a carrier, and at least one of the following additives: wetting agent, moisturizer, thickener, emulsifier, emollient, surfactant, antioxidant, stabilizer, fragrance, and preservative, wherein the cucumber seed oil extract accounts for 1 wt.% to 5 wt.% of the total mass of the cosmetic.

[0118] The carrier may be water or an organic solvent that is harmless to humans or the environment. For example, the organic solvent may be ethanol or a homologue of ethanol. The auxiliary agents in cosmetics are selected from propylene glycol, butylene glycol, 1,2-pentanediol, glycerin, carbomer, xanthan gum, coconut oil-caprylate / capric acid ester, squalane, resveratrol, coconut oil, olive oil, camellia oil, jojoba oil, lavender essential oil, jasmine essential oil, basil essential oil, clove essential oil, lemongrass essential oil, grapefruit essential oil, lemon essential oil, geranium essential oil, eucalyptus essential oil, peppermint essential oil, chamomile essential oil, chrysanthemum essential oil, sophora japonica essential oil, cornflower essential oil, plum blossom essential oil, rosin, dimethicone 350CS, Olivem 1000, glyceryl stearate, p-hydroxyacetophenone, and 1,2-hexanediol.

[0119] The cucumber seed oil extract of this application has a mass fraction of 1wt.% to 5wt.% in cosmetics. Within this range, it is beneficial for the cucumber seed oil extract to promote the proliferation of immortalized human keratinocytes and reduce the production of inflammatory factors, thus having a strong anti-inflammatory effect. At the same time, it can also promote the expression of filaggrin and tight junction protein genes, thus having a barrier repair effect, and can also avoid the adverse effects of excessive concentration.

[0120] Cosmetics can be formulated into various forms, including but not limited to creams, lotions, lotions, toners, sprays, patches, and masks.

[0121] Fifthly, this application provides a medicine comprising the above-mentioned cucumber seed oil extract or a cucumber seed oil extract prepared according to the preparation method of cucumber seed oil extract.

[0122] In some optional embodiments of this application, the medicament may further include acceptable adjuvants, including but not limited to any adjuvants, carriers, excipients, retention aids, sweeteners, diluents, preservatives, dyes / colorants, flavor enhancers, surfactants, wetting agents, dispersants, suspending agents, stabilizers, isotonic agents, solvents, or emulsifiers approved for use in humans or animals, and other carriers in various forms that do not have side effects on the pharmaceutical composition. The cucumber seed oil extract constitutes 1 wt.% to 5 wt.% of the total mass of the medicament.

[0123] For example, the excipients in the pharmaceutical product are selected from propylene glycol, mannitol, aminomethylpropanol, sorbitol, cellulose, ethyl cellulose, cellulose propionate, cellulose acetate propionate, cellulose acetate butyrate, methyl or ethyl acrylate, methyl methacrylate, xylitol, dextran, cyclodextrin, gelatin, gum arabic, corn starch, potato starch, hydroxymethyl cellulose, hydroxypropyl cellulose, carboxymethyl cellulose, polyvinylpyrrolidone, acetone, ethanol, methanol, 1,2-hexanediol, isopropanol, magnesium stearate, stearic acid, and calcium stearate. Glyceryl monostearate, ethylhexylglycerin, glyceryl tristearate, caprylic / capric triglyceride, polyethylene glycol, palmitic acid, ethylhexyl palmitate, aspartame, neotame, sodium saccharin, steviol glycoside, erythrose, glucose, sodium sucrose chloride, methylparaben, ethylparaben, propylparaben, phenoxyethanol, α-carotene, β-carotene, propylene glycol monolaurate, lauroyl polyoxyglyceride, linoleyl polyoxyglyceride, oleyl polyoxyglyceride or stearoyl polyoxyglyceride, microcrystalline cellulose, colloidal silica or combinations thereof.

[0124] In some optional embodiments of this application, the drug is used directly as a drug with anti-inflammatory and cell-repairing properties.

[0125] In some optional embodiments of this application, the drug is prepared by using cucumber seed oil extract as an additive to assist in anti-inflammatory and cell-repairing effects.

[0126] In some optional embodiments of this application, the drug may be prepared as a topical pharmaceutical dosage form including but not limited to ointments, creams, lotions, and patches.

[0127] In some optional embodiments of this application, the dosage form of the drug is selected from uncoated tablets, film-coated tablets, sugar-coated tablets, sausage-coated tablets, dispersible tablets, capsules, oral solutions, and oral suspensions.

[0128] Those skilled in the art can mix the cucumber seed oil extract of the present invention with the above-mentioned adjuvants according to any method known in the prior art, and any dosage form prepared is also known in the prior art.

[0129] For example, drugs with anti-inflammatory and cell-repairing properties can be formulated into a lip balm dosage form for use in cases of dry and inflamed lips caused by dryness or inflammation.

[0130] Example

[0131] The present application will be further illustrated by the following examples. Any simple modifications to the preparation method based on the concept of the present application are within the scope of protection claimed in this application. Unless otherwise specified, all raw materials and solvents used in the examples are commercially available products and can be obtained through commercial channels.

[0132] Example 1

[0133] A method for preparing cucumber seed oil extract, the method comprising:

[0134] S1. Provide dried cucumber seeds, including removing impurities other than the kernels of fresh cucumber seeds, then washing the cucumber seeds after impurity removal until the washing solution is clear and transparent, and then placing the cucumber seeds outdoors to dry, to obtain dried cucumber seeds with a moisture content of 4.5 wt.%.

[0135] S2. Seed oil extraction: Weigh 500 kg of dried cucumber seeds and add them to a screw oil press for cold pressing. The pressing temperature is controlled at 68℃~70℃. After pressing, 99.1 kg of yellow cucumber seed crude oil with white sediment is obtained.

[0136] S3. Seed oil purification: Cucumber crude seed oil was thoroughly mixed with 350L of purified water and heated to 70℃. The mixture was dispersed at 200rpm for 30 minutes and allowed to stand for 10 hours to obtain an oil-water two-phase liquid. The aqueous phase containing water-soluble organic acids was removed from the oil-water two-phase liquid to obtain 95.5 kg of oil phase liquid.

[0137] S4. Deodorization: Heat the oil phase liquid to 215°C.o C~220 o C. Stir at 200 rpm for 2 hours to deodorize, then cool to obtain deodorized cucumber seed crude oil;

[0138] S5. Degreasing: The deodorized cucumber seed crude oil is transferred to a cooling tank, with the temperature controlled at 2°C. o C~3 o C. After 3 days, the solid esters in the deodorized cucumber seed crude oil were removed, and the supernatant was taken to obtain 86.4 kg of defatted cucumber seed crude oil.

[0139] S6. The defatted cucumber seed crude oil was filtered once using a filter membrane with a pore size of 0.8 μm. Then, 100 g of anhydrous sodium sulfate was added to the filtrate and dispersed at 200 rpm for 30 min. The filtrate was then filtered a second time using a filter membrane with a pore size of 0.8 μm to obtain 83.3 kg of light yellow cucumber seed oil extract (H-1), with a yield of 16.66%.

[0140] Example 2

[0141] A method for preparing cucumber seed oil extract, the method comprising:

[0142] S1. Provide dried cucumber seeds, including removing impurities other than the cucumber kernels from fresh cucumber seeds, washing the cucumber kernels after impurity removal until the washing liquid is clear and transparent, and then placing the cucumber kernels into a blower dryer for drying at a temperature of 50°C for 8 hours to obtain dried cucumber seeds with a moisture content of 2.4 wt.%.

[0143] S2. Seed oil extraction: 500 kg of dried cucumber seeds were weighed and added to a pulverizer for pulverization. The pulverized seeds were then sieved through a 50-mesh sieve to obtain cucumber seed powder. Subsequently, the cucumber seed powder was subjected to supercritical CO2 extraction at an extraction pressure of 25 MPa, a flow rate of 30 L / h, an extraction temperature of 45℃, and an extraction time of 180 min to obtain 98 kg of slightly turbid yellow crude cucumber seed oil.

[0144] S3. Seed oil purification: Cucumber crude seed oil is thoroughly mixed with 350L of purified water and heated to 70℃. The mixture is then dispersed at 200rpm for 30 minutes and allowed to stand for 10 hours to obtain an oil-water two-phase liquid. The aqueous phase containing water-soluble organic acids is removed from the oil-water two-phase liquid to obtain 95.9kg of oil phase liquid.

[0145] S4. Deodorization: Heat the oil phase liquid to 225°C. o C~230 o C. Stir at 150 rpm for 3 hours to deodorize, then cool to obtain deodorized cucumber seed crude oil;

[0146] S5. Degreasing: The deodorized cucumber seed crude oil is transferred to a cooling tank, with the temperature controlled at 3°C. o C~5 o C. After 5 days, the solid esters in the deodorized cucumber seed crude oil were removed, and the supernatant was taken to obtain 89.6 kg of defatted cucumber seed crude oil.

[0147] S6. The defatted cucumber seed crude oil was filtered once using a filter membrane with a pore size of 0.8 μm. Then, 200 g of anhydrous sodium sulfate was added to the filtrate and dispersed at 200 rpm for 30 min. The filtrate was then filtered a second time using a filter membrane with a pore size of 0.8 μm to obtain 87.7 kg of light yellow cucumber seed oil extract (H-2), with a yield of 17.54%.

[0148] Comparative Example 1

[0149] This comparative example provides a method for preparing cucumber seed oil extract, including:

[0150] S1. Provide dried cucumber seeds, including removing impurities (excluding the cucumber kernels) from fresh cucumber seeds, washing the seeds after impurity removal until the washing solution is clear and transparent, and then air-drying the seeds outdoors to obtain dried cucumber seeds with a moisture content of 4.5 wt.%.

[0151] S2. Seed oil extraction: Weigh 500 kg of dried cucumber seeds and add them to a screw oil press for cold pressing. The pressing temperature is controlled at 65~70℃. After pressing, 99.3 kg of yellow cucumber seed crude oil with white sediment is obtained.

[0152] S3. Deodorization: The crude cucumber seed oil is heated to 215°C. o C~220 o C. Stir at 200 rpm for 2 hours to deodorize, then cool to obtain deodorized cucumber seed crude oil;

[0153] S5. Degreasing: The deodorized cucumber seed crude oil is transferred to a cooling tank, with the temperature controlled at 2°C. o C~3 o C. After 3 days, the solid esters in the deodorized cucumber seed crude oil were removed, and the supernatant was taken to obtain 95.6 kg of defatted cucumber seed crude oil.

[0154] S6. The defatted cucumber seed crude oil was filtered once using a filter membrane with a pore size of 0.8 μm. Then, 100 g of anhydrous sodium sulfate was added to the filtrate and dispersed at 200 rpm for 30 min. The mixture was then filtered a second time using a filter membrane with a pore size of 0.8 μm to obtain 93.2 kg of light yellow cucumber seed oil extract (D-1), with a yield of 18.64%. The difference between this comparative example and Example 1 is that seed oil purification was not performed.

[0155] Comparative Example 2

[0156] This comparative example provides a method for preparing cucumber seed oil extract, including:

[0157] S1. Provide dried cucumber seeds, including removing impurities (excluding the cucumber kernels) from fresh cucumber seeds, washing the seeds after impurity removal until the washing solution is clear and transparent, and then air-drying the seeds outdoors to obtain dried cucumber seeds with a moisture content of 4.6 wt.%.

[0158] S2. Seed oil extraction: 500 kg of dried cucumber seeds were weighed and added to a screw oil press for cold pressing. The pressing temperature was controlled at 68℃~70℃. After pressing, 98.5 kg of yellow cucumber seed crude oil with white sediment was obtained.

[0159] S3. Seed oil purification: Cucumber crude seed oil was mixed with 350L of purified water and heated to 70℃. The mixture was dispersed at 200rpm for 30 min and allowed to stand for 10 h to obtain an oil-water two-phase liquid. The aqueous phase containing water-soluble organic acids was removed from the oil-water two-phase liquid to obtain 95.1 kg of oil phase liquid.

[0160] S4. Deodorizing the seed oil: Heat the oil phase liquid to 218~220°C. o C. Stir at 200 rpm for 2 hours to deodorize, then cool to obtain deodorized cucumber seed crude oil;

[0161] S5. The deodorized cucumber seed crude oil was filtered once using a filter membrane with a pore size of 0.8 μm. Then, 100 g of anhydrous sodium sulfate was added to the filtrate and dispersed at 200 rpm for 30 min. The mixture was then filtered a second time using a filter membrane with a pore size of 0.8 μm to obtain 94.0 kg of light yellow cucumber seed oil extract (D-2), with a yield of 18.80%. The difference between this comparative example and Example 1 is that no defatting treatment was performed.

[0162] Test section

[0163] The acid value and fatty acid content of the cucumber seed oil extracts prepared in Examples 1-2 and Comparative Examples 1-2 were determined using the first method of the national food safety standard GB5009.229-2016 "Determination of Acid Value in Food" and the first method of GB5009.168-2016 "Determination of Fatty Acids in Food", respectively. The results are shown in Table 1.

[0164] Table 1. Comparison of acid value and fatty acid content of cucumber seed oil extract.

[0165]

[0166] From the preparation methods of cucumber seed oil extracts in Examples 1-2 and Comparative Examples 1-2, and the test data in Table 1, it can be seen that adding water helps to reduce the acid value of cucumber seed oil extracts, defatting helps to reduce the content of saturated fatty acids, and improves the quality level of oils.

[0167] Examples 3-4

[0168] Examples 3-4 each provide a cream, and the material ratios of each are shown in Table 2 below:

[0169] Table 2. Material proportions of the creams in Examples 3-4

[0170]

[0171] Note: The units for each component in Table 2 are grams (g).

[0172] Preparation Examples 3-4 contain formulations of cucumber seed oil extract, including:

[0173] Add component A to a water bath and heat to 75℃~80℃ to obtain solution A;

[0174] Add component B to the oil pan and heat to 75℃~80℃ to obtain solution B;

[0175] The solutions from group A and group B were mixed in an emulsifying pot and homogenized for 20 minutes to obtain an emulsion.

[0176] The emulsion was cooled to 45°C, component C was added, and the mixture was stirred for 30 minutes before being discharged and allowed to stand for 48 hours. It was then filled and packaged. The final product was a white to egg-yolk yellow cream, fine and uniform in texture. This cream can be used as a topical medication or skincare product.

[0177] Example 5

[0178] A toner comprising: 5g of cucumber seed oil extract prepared in Example 1; 10g of propylene glycol; 3g of cocoyl oleate / caprylate; 0.5g of jasmine essential oil; and water to a total weight of 100g.

[0179] Example 6

[0180] An anti-inflammatory spray comprises 5g of cucumber seed oil extract prepared in Example 2; 16g of ethanol; 4g of polyethylene glycol; 2g of lauroyl polyoxyglyceride; 1.5g of propylene glycol monolaurate; and water to a total mass of 100g.

[0181] Bioactivity test

[0182] 1. Cytotoxicity test

[0183] The cucumber seed oil extract (H-1) prepared in Example 1 was prepared into a solution with PBS at a volume percentage 10 times that of the concentration to be tested; immortalized human keratinocytes were cultured in a solution containing 10% (v / v) fetal bovine serum and 1% penicillin-antibody (1×10⁻⁶) 5 Immortalized human keratinocytes were cultured in DMEM medium containing 100 mg / L penicillin and 100 mg / L streptomycin at 37°C in a 5% CO2 incubator until the cell confluence reached 85%-95%. PBS, a phosphate buffer for cell culture, was prepared as follows: potassium dihydrogen phosphate (KH2PO4): 0.27 g; disodium hydrogen phosphate (Na2HPO4): 1.42 g; sodium chloride (NaCl): 8 g; potassium chloride (KCl): 0.2 g; add approximately 800 mL of deionized water and stir thoroughly to dissolve. Then, add concentrated hydrochloric acid to adjust the pH to 7.4, and finally bring the volume to 1 L.

[0184] Immortalized human keratinocytes in logarithmic growth phase were digested with trypsin at a concentration of 0.05% (v / v), and the digestion reaction was terminated with DMEM medium containing 10% (v / v) fetal bovine serum.

[0185] Use a cell counting chamber to adjust the number of cells per unit volume of the cell suspension to 2 × 10⁻⁶. 5 / ml, seeded into 96-well plates at a ratio of 200μL per well, and incubated at 37℃ and 5% CO2 for a certain period of time until the cell confluence reached 45%-60%; then remove the old culture medium, and add 200μL of serum-free culture medium containing the test sample at a volume concentration (v / v) of 0.25%, 0.50%, 1.00%, and 2.50% respectively to the corresponding test wells as the sample group, and perform 4 replicates for each volume concentration of the test solution.

[0186] The control group (BC) contained cells and was incubated with 200 μL of serum-free culture medium at 37°C and 5% CO2 for 24 h.

[0187] Then, 20 μL of CCK8 solution was added to each well of the sample group, blank group, and control group, and incubated for 3 hours. The absorbance value was measured at 450 nm, and the cell viability of each group was calculated.

[0188] (1) Cell viability (%) = (sample group - blank group) / (control group - blank group) * 100%;

[0189] (2) Cucumber seed oil extract raw material test concentration description: The final concentration of cucumber seed oil extract (H-1) sample provided in Example 1 in the culture medium.

[0190] In cytotoxicity tests, higher cell viability indicates lower cytotoxicity of the cucumber seed oil extract. The cytotoxicity test results of cucumber seed oil extract (H-1) against immortalized human keratinocytes are as follows: Figure 2 As shown: when the volume concentration of cucumber seed oil extract was 2.5% (v / v), the immortalized human keratinocytes had normal morphology and a relative cell viability of 76%. Therefore, according to the CCK8 test results, cucumber seed oil extract had no cytotoxicity to immortalized human keratinocytes in the volume concentration range of 2.5% (v / v).

[0191] 2. Cell scratch test

[0192] Cell migration assay: Immortalized human keratinocytes (HaCAT) in the logarithmic growth phase were collected and migrated at a rate of 2 × 10⁻⁶. 5 Cells were seeded at a density of 1 / ml into 24-well culture plates. The seeded plates were incubated at 37°C and 5% CO2 for 24 hours. Then, a 200μL pipette tip was used to create "damage" marks in the 24-well plates. Cells were washed three times with PBS to remove the marked cells. Samples were loaded according to Table 3, with FBS-free medium as the negative control, medium containing 10% FBS as the positive control, and medium without FBS and 2.5% (v / v) cucumber seed oil extract as the sample group. The cells were then incubated at 37°C and 5% CO2 for 24 hours, with three replicates per group. Cells were photographed using an inverted microscope during migration. Comparison images of the migration at the start of the migration test and after 48 hours are shown below. Figure 3 As shown. FBS stands for Fetal Bovine Serum.

[0193] Table 3. Cell migration experimental design table

[0194]

[0195] Combined with Table 3 and Figure 3 The comparison results show that in the cell migration experiment, the vertical spacing of the scratches in the negative control group was only 20% shorter after 48 hours compared to 0 hours; the vertical spacing of the scratches in the positive control group was 40% shorter after 48 hours compared to 0 hours. Compared with the negative and positive control groups, the vertical spacing of the scratches was shortened by 50% after 48 hours of treatment with cucumber seed oil extract at a volume concentration of 2.5% (v / v), indicating that the healing rate of immortalized human keratinocytes was increased. This proves that cucumber seed oil extract has a certain effect on promoting cell migration and repairing cell scratch damage.

[0196] 3. Anti-inflammatory and soothing efficacy experiment

[0197] Cell seeding: Mouse macrophages (i.e., RAW264.7 cells) were seeded at a rate of 1.5 × 10⁻⁶ cells / year. 5 Inoculate 100 cells / well into a 24-well plate and incubate overnight in a 37°C, 5% CO2 incubator;

[0198] Experimental groups: A control group, negative control group, positive control group, sample group, and solvent control group were set up. The sample group had three concentration gradients, with three replicates for each gradient.

[0199] Solution preparation: Prepare working solutions of cucumber seed oil extract according to the test concentration setting table. For NO content detection, prepare working solutions of cucumber seed oil extract with volume concentrations of 0.63% (v / v), 1.250% (v / v), and 2.5% (v / v); for TNF-α content detection, prepare working solutions of cucumber seed oil with volume concentrations of 1.250% (v / v) and 2.5% (v / v).

[0200] Sample feeding: Feed the cells when the cell deposition rate in the 24-well plate reaches 50%-60%; among which,

[0201] The control group consisted of culture medium containing no drugs.

[0202] Each negative control group was given a culture medium containing LPS.

[0203] The positive control group was given a culture medium containing LPS and the positive control drug dexamethasone (DEX).

[0204] Culture medium containing LPS and a corresponding volume concentration of cucumber seed oil extract working solution was added to each well of the sample group;

[0205] The solvent control group was supplemented with LPS-free culture medium;

[0206] LPS refers to lipopolysaccharide (LPS), with a mass concentration of 1 μg / mL. The concentration of LPS added to each group was the same.

[0207] Detection: After drug administration, the 24-well plate was placed in an incubator (37℃, 5% CO2) for 24 hours, and 500 μL of cell supernatant was collected for the determination of the levels of inflammatory factors NO and TNF-α.

[0208] Then, add DMEM-diluted CCK8 solution to each well, incubate at 37°C, and read the OD value at 450 nm; relative cell viability (%) = (sample group OD - control group OD) / (solvent control group OD - control group OD) * 100%.

[0209] 1) Effect of cucumber seed oil extract (H-1) on NO release from mouse macrophages (i.e., RAW264.7 cells)

[0210] like Figure 4 As shown, the experimental results indicate that:

[0211] (1) RAW264.7 cells overexpressed NO after LPS stimulation, reaching 5.68 μM;

[0212] (2) After treatment with cucumber seed oil extract at volume concentrations (v / v) of 2.5%, 1.25% and 0.63%, the expression level of NO in mouse macrophages decreased to 2.92 μM, 4.09 μM and 4.91 μM, respectively. This indicates that the cucumber seed oil extract can effectively act on LPS-stimulated RAW264.7 cells and alleviate cellular inflammation.

[0213] 2) Effect of cucumber seed oil extract (H-1) on TNF-α release from mouse macrophages (RAW264.7 cells)

[0214] like Figure 5 As shown, the experimental results indicate that:

[0215] (1) After LPS stimulation, the expression level of pro-inflammatory factor TNF-α in RAW26.47 cells was significantly increased to 638 pg / ml;

[0216] (2) After treatment with cucumber seed oil extract at concentrations of 2.5% and 1.25%, the synthesis of pro-inflammatory factor TNF-α was inhibited to varying degrees, and the expression level of TNF-α decreased to 583 pg / ml and 618 pg / ml, respectively. This indicates that the cucumber seed oil extract at this concentration can act on LPS-stimulated RAW264.7 cells and can effectively alleviate the cellular inflammatory response.

[0217] 4. Barrier Repair Efficacy Experiment

[0218] 1. UVB treatment of immortalized human keratinocytes (HaCaT cells)

[0219] (1) Immortalized human keratinocytes were cultured in a solution containing 10% fetal bovine serum and 1% penicillin-dextrose antibody (1×10⁻⁶). 5 Cells were cultured in DMEM medium containing 100 mg / L penicillin and 100 mg / L streptomycin. Cells were grown at 37°C in a 5% CO2 incubator until cell confluence reached 85%-95%.

[0220] (2) Digest logarithmic growth phase cells with 0.05% trypsin and terminate the digestion reaction with DMEM medium containing 10% serum;

[0221] (3) Count the cells using a cell counting chamber, dividing the cells into groups of 2 × 10⁻⁶. 5 Cells were seeded at a density of / ml into 6-well plates and incubated at 37°C and 5% CO2 for a certain period of time until the cell confluence reached 60%-70%.

[0222] (4) Experimental groups: CTR control group (only DMEM added, no UVB irradiation), model control group (UVB+DMEM), and sample group (sample+UVB+DMEM, the sample refers to the cucumber seed oil extract of Example 1 with a final concentration of 1% (v / v)).

[0223] (5) Replace the original culture medium in the 6-well plate with serum-free medium, starve the plate for two hours, add the samples from each group in (4) and treat for 4 hours, then replace the medium with PBS and use 30 mJ / cm 2 Immortalized human keratinocytes were irradiated with UVB and cultured for another 24 hours.

[0224] 2. RNA extraction

[0225] (1) Cell sampling (operation on ice): The cells cultured in 6-well plates were washed twice with pre-cooled sterile PBS, TRIzol was added, 500 μL was added to each well, and the cells were gently pipetted to collect the cell lysate in 1.5 ml EP tubes.

[0226] (2) Pre-cool the high-speed centrifuge at 4℃, centrifuge at 12000g for 10min, take the supernatant into a new RNase-FREE 1.5ml EP tube, and discard the tissue precipitate;

[0227] (3) Add 200 μL of chloroform to the clear liquid, shake it up and down 15 times to mix the liquid thoroughly, keep the cap closed and let it stand at room temperature for 5 minutes to allow it to separate naturally.

[0228] (4) Centrifuge at 12000g for 15 min at 4℃, and carefully aspirate the upper aqueous phase into a new RNase-FREE 1.5ml EP tube. Pay special attention to avoid touching the central precipitate to prevent protein contamination.

[0229] (5) Add 500 μL of isopropanol to the supernatant, gently invert to mix, keep the cap closed and let stand at room temperature for 5 min to allow the mRNA to be fully extracted;

[0230] (6) Centrifuge at 12000g for 10 min at 4℃, discard the supernatant and add 75% ethanol solution prepared with DEPC water, and gently invert to wash the mRNA precipitate;

[0231] (7) Centrifuge at 7500g for 5 min at 4℃, discard the supernatant and wash the mRNA precipitate again with 75% ethanol solution;

[0232] (8) Centrifuge at 7500g for 5 minutes at 4℃, discard the supernatant and absorb as much residual liquid as possible, and let stand at room temperature for 30-60 minutes to evaporate the residual ethanol.

[0233] (9) Add an appropriate amount of DEPC water to dissolve the mRNA, determine the concentration, and perform reverse transcription according to the instructions of the reverse transcription kit.

[0234] 3. Reverse transcription PCR

[0235] System (20 μl): 10× buffer 2 μl, 2.5 mM dNTP 2 μl, DNA polymerase (Taq) 0.5 μl, primer 2 μl, template (cDNA obtained from reverse transcription diluted 10 times as template) 2 μl - 10 μl of ultrapure water to make up the system to 20 μl;

[0236] Reaction conditions: Pre-denaturation 94℃ 5min; 30 cycles; denaturation 94℃ 30s, annealing 60℃ 30s, extension 72℃ 30s; post-extension 72℃ 10min;

[0237] 5. Quantitative Real-Time PCR (qPCR) assay

[0238] System (20 μl): 10 μl of 2×SYBR, 2 μl-5 μl of template (cDNA obtained by reverse transcription diluted 10 times as template), 1 μl-2 μl of primer, and ultrapure water to make up the system to 20 μl;

[0239] Reaction conditions: Pre-denaturation 94℃ 5min; 40 cycles: denaturation 94℃ 30s, annealing 60℃ 30s, extension 72℃ 30s (real-time fluorescence photography); melting curve 94℃ 30s, 60℃ 30s, 72℃ 1s (real-time fluorescence photography during heating process).

[0240] The following are the primers used for reverse transcription PCR and quantitative real-time PCR (QPCR) experiments:

[0241]

[0242] Experimental conclusions: such as Figure 6 and 7 As shown, cucumber seed oil extract at a volume concentration of 1% (v / v) can significantly promote the expression level of FLG (filaggrin) and ZO-1 (Zona occludens protein 1) in the mRNA of UVB-damaged immortalized human keratinocytes, and has a certain repair effect on UVB-damaged immortalized human keratinocytes.

[0243] The above description is merely a specific implementation of this application. Those skilled in the art will clearly understand that, for the sake of convenience and brevity, the specific working process described above can be referred to the corresponding process in the foregoing method embodiments, and will not be repeated here. It should be understood that the protection scope of this application is not limited thereto. Any person skilled in the art can easily conceive of various equivalent modifications or substitutions within the technical scope disclosed in this application, and these modifications or substitutions should all be covered within the protection scope of this application.

Claims

1. The use of a cucumber seed oil extract in the preparation of cosmetics for repairing UVB-damaged keratinocytes, characterized in that, The cucumber seed oil extract comprises, by mass percentage: 73%–76% linoleic acid, 10%–12% palmitic acid, 8%–9% oleic acid, 2%–3% stearic acid, and 2%–3% linolenic acid; The method for preparing the cucumber seed oil extract includes: providing dried cucumber seeds; seed oil extraction, including pressing the dried cucumber seeds or pulverizing them and then extracting them with supercritical carbon dioxide to obtain crude cucumber seed oil; seed oil purification, including removing water-soluble organic acids from the crude cucumber seed oil to obtain an oil phase liquid; deodorization, including deodorizing the purified oil phase liquid to obtain deodorized crude cucumber seed oil; defatting, including maintaining the deodorized crude cucumber seed oil at a temperature of 2-5°C for 3-5 days, removing the solid esters from the deodorized crude cucumber seed oil, and taking the supernatant to obtain defatted crude cucumber seed oil; and removing impurities from the defatted crude cucumber seed oil and drying it to obtain the cucumber seed oil extract.

2. The use according to claim 1, characterized in that, The dried cucumber seeds provided include: Remove impurities other than the cucumber seed kernel from the cucumber seeds. Wash the cucumber seeds after removing impurities until the washing solution is clear and transparent. The washed cucumber seeds are dried to obtain dried cucumber seeds; and / or The seed oil extraction is performed using cold pressing, including: The dried cucumber seeds are pressed at 40℃ to 70℃ to obtain crude cucumber seed oil; or The seed oil extraction method employs supercritical CO2 extraction, including: The dried cucumber seeds are crushed and sieved to obtain cucumber seed powder with a particle size of 10 to 50 mesh. Cucumber seed powder was subjected to supercritical CO2 extraction to obtain crude cucumber seed oil; and / or The seed oil purification includes: The crude cucumber seed oil was mixed with purified water at a volume ratio of 1:2 to 5, and then dispersed and allowed to stand at 70°C to 80°C to obtain an oil-water two-phase liquid. Remove the aqueous phase from the oil-water two-phase liquid to obtain the oil phase liquid; and / or The deodorization process includes heating the oil phase liquid to 210℃~230℃, stirring at 100rpm~200rpm for 2~3 hours to deodorize, and then cooling to obtain deodorized cucumber seed crude oil; and / or The process of removing impurities and drying the defatted cucumber seed crude oil includes: The defatted cucumber seed crude oil is filtered once, and then 0.1wt% to 0.5wt% of a chemical drying agent is added to the filtrate for dispersion, followed by a second filtration to obtain cucumber seed oil extract.

3. The use according to claim 2, characterized in that, The cucumber seeds, after impurities have been removed, are washed until the washing solution is clear and transparent, with the water temperature controlled between 20℃ and 50℃; and / or The seed oil extraction process using supercritical CO2 extraction parameters meets the following requirements: pressure of 25 MPa~50 MPa, flow rate of 25 L / h~45 L / h, temperature of 30℃~55℃, and extraction time of 90 min~180 min; and / or The seed oil purification process includes: mixing the crude cucumber seed oil with purified water at a volume ratio of 1:2 to 5, heating to 70°C to 80°C, dispersing at 100 rpm to 200 rpm for 2 to 3 hours, and then allowing it to stand for 10 to 12 hours to obtain an oil-water two-phase liquid; removing the aqueous phase containing water-soluble organic acids to obtain the oil phase liquid; and / or The purification and drying of the defatted cucumber seed crude oil includes: filtering the defatted cucumber seed crude oil once using a 0.1μm~0.8μm filter membrane; adding 0.1wt%~0.5wt% of a chemical drying agent to the filtrate; dispersing the filtrate at 100rpm~200rpm for 30min~60min; and then filtering the dried product a second time using a 0.1μm~0.8μm filter membrane to obtain a cucumber seed oil extract with a yield of 15%~18%; and / or The chemical drying agent is selected from anhydrous magnesium sulfate, anhydrous sodium sulfate, anhydrous calcium chloride, quicklime, or a combination thereof.

4. The use according to claim 1, characterized in that, The cosmetic also includes at least one of the following additives: humectant, moisturizer, thickener, emulsifier, emollient, surfactant, antioxidant, stabilizer, and preservative. The cucumber seed oil extract accounts for 1 wt% to 5 wt% of the total mass of the cosmetic.

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