Method for promoting early sprouting of sugarcane by using trichostatin a
By soaking sugarcane seed stalks in an aqueous solution of qugu antibacterial agent A, combined with treatment with fungicides and adhesives, the problem of unstable sugarcane budding was solved, resulting in earlier budding and improved uniformity of budding.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
- Filing Date
- 2023-12-01
- Publication Date
- 2026-05-12
AI Technical Summary
Existing technologies are not always effective in promoting early budding of sugarcane, and the budding uniformity is low, making them susceptible to external conditions.
Sugarcane seed stalks were soaked in an aqueous solution of 0.1-1 μM/L qugu antibacterial agent A, combined with fungicide and adhesive treatment. The soaking temperature was 22-26℃, the pH was 6-8, and the soaking time was 10-15 hours. The buds were facing upwards when sowing.
It achieved a sugarcane sprouting time that was more than 3 days earlier, with a high degree of uniformity in sprouting and stable results.
Smart Images
Figure CN117652499B_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of sugarcane planting technology, and in particular to a method for promoting early budding of sugarcane using qugu antibacterial agent A. Background Technology
[0002] Sugarcane, also known as sugarcane or sweet potato cane, is a perennial herbaceous subtropical crop widely cultivated in Guangxi, Guangdong, Yunnan, and other regions of my country. Rich in nutrients and with a wide range of uses, sugarcane can be eaten directly as a sweet and delicious fruit. It also has significant economic value and broad application prospects in industry, being used in sugar refining, synthetic paper production, sugarcane vinegar production, and the production of bioethanol, alcoholic gasoline, and even for power generation from sugarcane bagasse. Sugarcane sugar accounts for approximately 94% of my country's total sugar production, making it a crucial economic crop in my country's tropical and subtropical regions and a vital source of income for farmers.
[0003] Studies have shown that earlier sprouting in sugarcane is beneficial for the growth of its main stem and also promotes more tillering, thus increasing sugarcane yield. Under natural conditions, sugarcane typically takes 7-10 days to sprout. To encourage earlier sprouting, current production methods mainly involve treatment with lime water, warm water, or the auxin sodium naphthaleneacetate (NAA) or gibberellin. Lime water and warm water treatments slightly advance sprouting, but result in lower uniformity. While NAA or gibberellin can accelerate sprouting, their effectiveness is easily affected by external conditions such as light and temperature, leading to variations in results.
[0004] Therefore, it is of great significance to find a stable method to promote early budding of sugarcane. Summary of the Invention
[0005] To address the above shortcomings, this invention provides a method for promoting early bud break in sugarcane using qugu antibacterial agent A. This method can advance the bud break time by more than 3 days and achieve a high degree of uniformity in bud break. The specific technical solution is as follows:
[0006] A method for promoting early budding of sugarcane using qugu styrax A involves soaking sugarcane seed stalks in a 0.1-1 μM / L qugu styrax A aqueous solution before conventional sowing. Sowing with the buds facing upwards facilitates emergence.
[0007] Furthermore, the purity of the said trogostatin A is ≥98%.
[0008] Furthermore, the soaking temperature is 22-26℃, and the pH is 6-8.
[0009] Furthermore, the soaking time is 10-15 hours.
[0010] Furthermore, the method also includes adding a bactericide and an adhesive to the aqueous solution of the quabuphenanthrene A.
[0011] Furthermore, the fungicide is one of prochloraz, carbendazim, or difenoconazole.
[0012] Furthermore, the adhesive is mainly composed of polyvinyl alcohol and starch-based substances mixed in a weight ratio of 1:0.1-0.3.
[0013] Furthermore, the amount of the bactericide added is 0.5-1% of the weight of the aqueous solution of qugu septica.
[0014] Furthermore, the amount of the adhesive added is 1-4% of the weight of the quabuphenanthrene A aqueous solution.
[0015] Trichostatin A is an effective histone deacetylase (HDAC) inhibitor and an antifungal antibiotic with cell differentiation-inducing properties.
[0016] Polyvinyl alcohol is an organic compound with hydrophilic and film-forming properties.
[0017] Soaking sugarcane seed stalks in an aqueous solution of qugu antibacterial agent A is generally done before sowing. When soaking, pay attention to the safety of the drug and do not increase the dosage without authorization. After soaking, the seed stalks can be used for sowing immediately.
[0018] Compared with existing technologies, the beneficial effects of this invention are: the method of this invention can advance the budding time of sugarcane by more than 3 days, and the budding uniformity is higher. Specifically, qugu styraxanthin A can break dormancy and promote sugarcane cell differentiation; the fungicide can help kill harmful bacteria on the sugarcane seed stalk; and the adhesive can keep the seed stalk moist and allow qugu styraxanthin A to adhere to the seed stalk, promoting the long-lasting effect of qugu styraxanthin A, thereby promoting earlier budding. Attached Figure Description
[0019] To more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings used in the description of the embodiments will be briefly introduced below.
[0020] Figure 1 This is a diagram showing the germination status of the planted plant on the 6th day after planting in Example 1 (the left row is the control group, and the right row is the experimental group). Detailed Implementation
[0021] The specific embodiments of the present invention will be described in detail below, but it should be understood that the scope of protection of the present invention is not limited to the specific embodiments.
[0022] The purity of trogostatin A used in the following examples is ≥98%.
[0023] Example 1
[0024] Sugarcane seed stalks of variety Guitang 42 were selected as the experimental subject. An experimental group and a control group were established. Before planting, the experimental group was soaked in a 0.1 μM / L solution of quaternary ammonium chloride (QMAC), while the control group was soaked in a solution containing the same concentration of QMAC solvent (anhydrous ethanol). The soaking temperature for both groups was 22℃, the pH was 6, and the soaking time was 10 hours. Six replicates were set for both groups (replicas in the experimental group were denoted as TSA1 to TSA6; replicates in the control group were denoted as CK1 to CK6). At sowing, the buds were all facing upwards. The planting date was March 14, 2022. The same water and fertilizer management was implemented after sowing. The germination time of each group was observed and recorded, and the germination length was measured 10 days after planting. The results are shown in Table 1.
[0025] Table 1 Comparison of budding status in each group
[0026]
[0027]
[0028] The table above shows that soaking the seed stems in an aqueous solution of qugu antibacterial agent A is more effective.
[0029] Example 2
[0030] Sugarcane seed stalks of variety Guitang 42 were selected as the research object. An experimental group and a control group were set up. Before planting, the experimental group was soaked in a 1 μM / L solution of quaternary ammonium chloride A, while the control group was soaked in a solution containing 1 μM / L quaternary ammonium chloride A solvent (anhydrous ethanol). The soaking temperature for both groups was 26℃, the pH was 8, and the soaking time was 15 h. Six replicates were set for both groups (6 replicates for the experimental group were denoted as TSA1 to TSA6; 6 replicates for the control group were denoted as CK1 to CK6). At sowing, the buds were all facing upwards, and the planting date was March 14, 2022. The same water and fertilizer management was implemented after sowing, and the germination time of each group was observed and recorded. The germination length was measured 10 days after planting. The results are shown in Table 2.
[0031] Table 2 Comparison of budding status in each group
[0032]
[0033]
[0034] Example 3
[0035] The seed stalks of sugarcane variety Guitang 42 were selected as the experimental subjects, and experimental groups, control group 1, control group 2, control group 3 and blank group were set up.
[0036] Experimental group: The seed stems were soaked in a 0.1 μM / L solution of qugu succinate A before planting.
[0037] Control group 1: Before planting, the seed stems were soaked in a solution of 0.1 μM / L qugu styrax A, a fungicide, and an adhesive. The fungicide was imazalil, and the amount added was 0.5% of the weight of the qugu styrax A solution. The adhesive was a mixture of polyvinyl alcohol and starch in a weight ratio of 1:0.1, and the amount added was 0.5% of the weight of the qugu styrax A solution.
[0038] Control group 2: Before planting, the seed stems were soaked in a 0.1 μM / L solution of qugu septic acid A and a fungicide; the fungicide was imazalil, and the amount added was 0.5% of the weight of the qugu septic acid A solution.
[0039] Control group 3: Before planting, the seed stems were soaked in a 0.1 μM / L solution of qugu styraxanthin A and an adhesive. The adhesive was a mixture of polyvinyl alcohol and starch in a weight ratio of 1:0.1 and was added at 0.5% of the weight of the qugu styraxanthin A solution.
[0040] Blank group: No treatment was performed on the seed stems before planting.
[0041] The soaking temperature for both the experimental and control groups was 26℃, the pH was 8, and the soaking time was 15 hours. Six replicates were set for each group. At sowing, the buds were all facing upwards, and the planting date was March 14, 2022. The same water and fertilizer management was implemented after sowing. Germination time was observed and recorded for each group, and the germination length was measured 10 days after planting, and the average value was calculated. The results are shown in Table 3.
[0042] Table 3 Comparison of budding status in each group
[0043]
[0044] The data in the table show that soaking the seed stems in a 0.1 μM / L solution of qugu antibacterial agent A, along with a fungicide and an adhesive, before planting yields the best results.
[0045] Example 4
[0046] The sugarcane seed stalks of Guitang 42 were selected as the experimental subjects, and experimental groups, control group 1, control group 2, control group 3, control group 4, control group 5 and blank group were set up.
[0047] Experimental group: The seed stems were soaked in a 0.1 μM / L solution of qugu succinate A before planting.
[0048] Control group 1: Before planting, the seed stems were soaked in a solution of 0.1 μM / L qugu septic acid A, a fungicide, and an adhesive. The fungicide was carbendazim, and the amount added was 1% of the weight of the qugu septic acid A solution. The adhesive was a mixture of polyvinyl alcohol and starch in a weight ratio of 1:0.3, and the amount added was 1% of the weight of the qugu septic acid A solution.
[0049] Control group 2: Before planting, the seed stems were soaked in a 0.1 μM / L solution of qugu septic acid A and a fungicide; the fungicide was carbendazim, and the amount added was 1% of the weight of the qugu septic acid A solution.
[0050] Control group 3: Before planting, the seed stems were soaked in a 0.1 μM / L solution of qugu styraxanthin A and an adhesive. The adhesive was a mixture of polyvinyl alcohol and starch in a weight ratio of 1:0.3 and was added at 1% of the weight of the qugu styraxanthin A solution.
[0051] Control group 4: The seed stems were soaked in a 50 mg / L oxytetracycline GA3 solution before planting.
[0052] Control group 5: The seed stems were soaked in warm water at 40℃ before planting.
[0053] Blank group: No treatment was performed on the seed stems before planting.
[0054] Both the experimental and control groups had six replicates. At sowing, the buds were all facing upwards, and the planting date was March 14, 2022. The same water and fertilizer management was implemented after sowing. Germination time was observed and recorded for each group, and the germination length was measured 10 days after planting, and the average value was calculated. The results are shown in Table 4.
[0055] Table 4 Comparison of budding status in each group
[0056]
[0057] The data in the table show that the seed stem treatment method of the present invention is superior to the traditional treatment method (control group 4 and control group 5), that is, the buds sprout earlier, the buds are longer, and the buds are more uniform.
[0058] The foregoing description of specific exemplary embodiments of the invention is for illustrative and explanatory purposes. These descriptions are not intended to limit the invention to the precise forms disclosed, and it will be apparent that many changes and variations can be made in accordance with the foregoing teachings. The exemplary embodiments were chosen and described in order to explain the specific principles of the invention and its practical application, thereby enabling those skilled in the art to implement and utilize various different exemplary embodiments of the invention, as well as various different choices and variations. The scope of the invention is intended to be defined by the claims and their equivalents.
Claims
1. A method for promoting early bud break in sugarcane using quaternary antibacterial agent A, characterized in that, Sugarcane seed stalks are soaked in a 0.1-1 μM / L solution of qugu septic acid A before conventional planting. The qugu septic acid A solution contains 0.5-1% by weight of a fungicide and 1-4% by weight of an adhesive. The fungicide is one of prochloraz, carbendazim, or difenoconazole. The adhesive is mainly composed of polyvinyl alcohol and starchy substances mixed in a weight ratio of 1:0.1-0.
3.
2. The method for promoting early bud break in sugarcane using quaternary antibacterial agent A according to claim 1, characterized in that, The purity of the quabustatin A is ≥98%.
3. The method for promoting early bud break in sugarcane using quaternary antibacterial agent A according to claim 1, characterized in that, The soaking temperature is 22-26℃, and the pH is 6-8.
4. The method for promoting early bud break in sugarcane using quaternary antibacterial agent A according to claim 1, characterized in that, The soaking time is 10-15 hours.