A method for establishing a largemouth bass model that simulates the susceptibility of iron overload pathogens using inorganic iron.

By adding FeSO4·7H2O to the feed of juvenile largemouth bass, an iron overload was simulated, and a pathogen susceptibility model was established. This solved the problem of the lack of model establishment in the existing technology, achieved significant pathological characteristics and high stability, and supported the study of pathogen infection mechanisms in aquatic animals.

CN117694276BActive Publication Date: 2026-05-26GUANGDONG LABORATORY OF SOUTHERN OCEAN SCIENCE AND ENGINEERING (GUANGZHOU) +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
GUANGDONG LABORATORY OF SOUTHERN OCEAN SCIENCE AND ENGINEERING (GUANGZHOU)
Filing Date
2023-12-15
Publication Date
2026-05-26

AI Technical Summary

Technical Problem

The lack of methods to establish a largemouth bass model of Aeromonas hydrophila susceptibility through dietary intervention affects research on pathogen infection mechanisms in aquatic animals and the development of therapeutic drugs.

Method used

By adding FeSO4·7H2O to the diet of juvenile largemouth bass, an iron overload was simulated, and a pathogen susceptibility model was established. This included a feeding period of 14–56 days and a challenge experiment with Aeromonas hydrophila, measuring changes in immune regulation, oxidative stress, and gut microbiota.

Benefits of technology

A susceptibility model for iron overload pathogens with high stability and significant pathological characteristics was successfully established, which significantly reduced survival rate, increased tissue iron content and oxidative stress, and altered immune regulation and gut microbiota, supporting research on the infection mechanism of pathogens in aquatic animals.

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Abstract

This invention discloses a method for establishing a largemouth bass model susceptibility to iron overload pathogens using inorganic iron, belonging to the field of biotechnology. The method includes: a) after acclimatizing 4-5cm largemouth bass to a normal diet for one week, the normal diet is replaced with a FeSO4·7H2O-supplemented diet (FeSO4·7H2O supplementation amount: 800-3200 mg / kg), while the control group continues to be fed a normal diet; b) after feeding the FeSO4·7H2O-supplemented diet for 14-56 days, the mortality rate from Aeromonas hydrophila infection increases, indicating successful establishment of the largemouth bass model susceptibility to pathogens. This method for establishing a largemouth bass model susceptibility to iron overload pathogens using inorganic iron has advantages such as simple operation, high success and survival rates, good stability, and obvious pathological characteristics.
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