Embryonic stem cell bionic culture device

Abstract

The invention discloses an embryonic stem cell bionic culture device, which relates to the technical field of cell culture, and comprises a centrifugal mechanism installed in a shell and a liquid inlet mechanism installed on the centrifugal mechanism. The centrifugal mechanism comprises a safety component and a support. A rotating shaft is rotatably installed on the support. A protection spring is sleeved on the rotating shaft. The first end of the protection spring is connected to the rotating shaft, and the second end of the protection spring is connected to a clamping ring. The clamping ring is slidably installed on the rotating shaft. The rotating shaft is rotatably connected to a mounting plate. A centrifugal platform is inserted on the mounting plate. The safety component comprises a fixing ring rotatably connected to the mounting plate, and the fixing ring is connected to a protective cover. The invention designs the centrifugal mechanism and the liquid inlet mechanism, drips embryonic stem cell culture medium into frozen embryonic stem cells, and centrifuges the cells, so that the experimental efficiency is high.

Description

Technical Field

[0001] The invention relates to the technical field of cell culture, and in particular to a bionic culture device for embryonic stem cells. Background Art

[0002] Embryonic stem cells exist in early embryos and have the potential to differentiate into any type of cell in an adult animal and further form all tissues and organs of the body and even individuals. The basic process of human embryonic stem cell subculture can be divided into reagent preparation, determination of subculture timing, collagenase treatment, collection and breakup of clones, centrifugal subculture, cryopreservation, and recovery. During recovery, the frozen embryonic stem cells need to be taken out of liquid nitrogen and immersed in a water bath, and then the cryopreservation tubes are taken out of the water bath and disinfected and dried. The cells are transferred from the cryopreservation tubes into centrifuge tubes, and embryonic stem cell culture medium is added dropwise, followed by centrifugation. The above process has a low degree of automation and low experimental efficiency.

[0003] The Chinese invention patent with publication number CN108018209A provides a cell and strain culture device, including a substrate A, a containing chamber A, a spiral tube, a channel tube, a control valve, and a first connecting tube. The patent adopts a water bath method, has a simple structure, can provide a constant temperature environment, and is conducive to cell and strain culture. However, the patent cannot solve the technical problems that the present invention can solve, so the present invention provides an embryonic stem cell bionic culture device. Summary of the invention

[0004] The present invention aims at the defects of the prior art and provides an embryonic stem cell bionic culture device to overcome the problems in the prior art.

[0005] The technical solution adopted by the present invention is: an embryonic stem cell bionic culture device, including a centrifugal mechanism installed in a shell and a liquid inlet mechanism installed on the centrifugal mechanism, the centrifugal mechanism includes a safety component and a support, a rotating shaft is rotatably installed on the support, a protection spring is sleeved on the rotating shaft, the first end of the protection spring is connected to the rotating shaft, the second end of the protection spring is connected to the clamping ring, the clamping ring is slidably installed on the rotating shaft, the rotating shaft is rotatably connected to the mounting disk, the centrifugal platform is inserted on the mounting disk, the safety component includes a fixed ring rotatably connected to the mounting disk, the fixed ring is connected to the protective cover, an insert block is slidably installed on the fixed ring, the insert block cooperates with the groove on the mounting disk, there are multiple insert blocks and they are evenly distributed, each insert block is rotatably connected to a sliding column respectively, the sliding column is slidably installed on the safety swivel, the safety swivel rotates around the axis, and the safety swivel is rotatably connected to the fixed ring.

[0006] Furthermore, the safety swivel is coaxially fixedly connected to the large gear ring, the large gear ring is meshed with the safety gear, the safety gear is coaxially fixedly connected to the knob, and the knob is rotatably mounted on the housing.

[0007] Furthermore, a liquid inlet mechanism is installed on the protective cover, and the liquid inlet mechanism includes a matching tube fixedly installed on the protective cover, a slider is provided on the matching tube, the slider on the matching tube is slidably installed in the spiral groove of the spiral rod, a return spring is sleeved on the spiral rod, the first end of the return spring is connected to the spiral rod, and the second end of the return spring is connected to the matching tube.

[0008] Furthermore, a lower guide plate is fixedly mounted on the protective cover, and a plurality of slides are evenly and slidably mounted on the lower guide plate, each slide is respectively connected to a slide rod, and the slide rod is slidably mounted on the upper guide plate, and the upper guide plate is fixedly connected to the rotating frame.

[0009] Furthermore, a material drop opening is arranged on the lower guide plate, and under normal conditions, adjacent slides are attached together and block the material drop opening.

[0010] Furthermore, the rotating frame is rotatably installed in the protective cover, the rotating frame is slidably matched with the mounting rod, and the mounting rod is fixedly arranged on the spiral rod.

[0011] Furthermore, the first end of the spiral rod is connected to the pressure rod, and the second end of the spiral rod is connected to the drip tube.

[0012] Furthermore, an observation window is provided on the protective cover.

[0013] Compared with the prior art, the present invention has the following advantages: (1) by pressing the pressure rod, the spiral rod is pressed down, and the return spring is compressed. The spiral rod is matched with the slider thread on the matching cylinder. When the spiral rod moves down, it rotates, driving the installation rod to rotate. The installation rod drives the rotating frame to rotate, driving the upper guide plate to rotate, driving the sliding rod to slide on the upper guide plate, driving the sliding plate to slide on the lower guide plate, so that multiple sliding plates are separated, exposing the drop port, and the drop tube drips embryonic stem cell culture medium into the centrifuge tube; (2) under normal conditions, the clamping ring is pressed against the installation plate under the elastic force of the protection spring, so that the installation plate The mounting plate contacts the rotating shaft, and the starting motor drives the driving gear to rotate, which drives the large gear to rotate, drives the small gear ring to rotate, and the small gear ring drives the rotating shaft to rotate. The rotating shaft drives the clamping ring and the mounting plate to rotate through the elastic pressure of the protection spring, and the mounting plate drives the centrifugal platform to rotate to perform centrifugation; (3) The present invention lifts the protective cover, takes out the centrifuge tube for subsequent operations, and then takes out the centrifuge platform for cleaning and disinfection. The position of the mounting plate and the centrifuge platform is limited by the plug-in block inserted into the groove of the mounting plate, so as to avoid the machine from suddenly operating when taking the centrifuge tube or cleaning the device subsequently, which affects the safety of the experiment. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 It is a schematic diagram of the overall structure of the present invention.

[0015] Figure 2 It is a schematic diagram of the local structure of the centrifugal mechanism of the present invention.

[0016] Figure 3 The partial structure diagram of the centrifugal mechanism and liquid inlet mechanism of the present invention is shown in FIG. Figure 1 .

[0017] Figure 4 It is a partial cross-sectional structural schematic diagram of the centrifugal mechanism of the present invention.

[0018] Figure 5 The partial explosion structure of the centrifugal mechanism of the present invention is shown in FIG. Figure 1 .

[0019] Figure 6 The partial explosion structure of the centrifugal mechanism of the present invention is shown in FIG. Figure 2 .

[0020] Figure 7 The partial structure diagram of the centrifugal mechanism and liquid inlet mechanism of the present invention is shown in FIG. Figure 2 .

[0021] Figure 8 The local structure of the liquid inlet mechanism of the present invention is shown in FIG. Figure 1 .

[0022] Fig. 9 The local structure of the liquid inlet mechanism of the present invention is shown in FIG. Figure 2 .

[0023] Fig.10 It is a partial cross-sectional structural schematic diagram of the liquid inlet mechanism of the present invention.

[0024] 1-centrifugal mechanism; 2-liquid inlet mechanism; 101-motor; 102-support; 103-driving gear; 104-large gear; 105-knob; 106-small gear ring; 107-rotating shaft; 108-protective spring; 109-pressure ring; 110-safety swivel; 111-large gear ring; 112-sliding column; 113-insertion block; 114-mounting plate; 115-fixing ring; 116-centrifugal platform; 117-protective cover; 118-safety gear; 201-pressure rod; 202-reset spring; 203-screw rod; 204-matching cylinder; 205-mounting rod; 206-rotating frame; 207-upper guide plate; 208-sliding rod; 209-lower guide plate; 210-sliding sheet; 211-drip tube. Implementation

[0025] The present invention will be further described in detail below in conjunction with the accompanying drawings and specific preferred embodiments.

[0026] Example: Refer to Figure 1-Figure 10The embryonic stem cell bionic culture device shown in the figure comprises a centrifugal mechanism 1 installed in a shell and a liquid inlet mechanism 2 installed on the centrifugal mechanism 1. The centrifugal mechanism 1 comprises a safety component, a motor 101, a support 102, a driving gear 103, a large gear 104, a small gear ring 106, a rotating shaft 107, a protective spring 108, a clamping ring 109, a mounting plate 114, a centrifugal platform 116, and a protective cover 117. The support 102 is rotatably mounted with a rotating shaft 107, and the rotating shaft 107 and the small gear ring 106 are connected. The ring 106 is fixedly connected, the small ring gear 106 is meshed with the large gear 104, the large gear 104 is rotatably mounted on the support 102, the large gear 104 is meshed with the driving gear 103, the driving gear 103 is connected to the output shaft of the motor 101, the motor 101 is mounted on the support 102, the support 102 is mounted on the housing, and a protective spring 108 is sleeved on the rotating shaft 107, the first end of the protective spring 108 is connected to the rotating shaft 107, and the second end of the protective spring 108 is connected to the pressing The rotating shaft 107 is connected with the ring 109, the clamping ring 109 is slidably mounted on the rotating shaft 107, the protection spring 108 is in a compressed state, the rotating shaft 107 is rotatably connected with the mounting plate 114, the clamping ring 109 contacts the mounting plate 114, the centrifugal platform 116 is inserted on the mounting plate 114, and the safety assembly includes a knob 105, a safety swivel 110, a large gear ring 111, a sliding column 112, an insert 113, a fixing ring 115, and a safety gear 118; the fixing ring 115 is rotatably connected with the mounting plate 114 The fixing ring 115 is rotatably connected to the protective cover 117, and an insert block 113 is slidably installed on the fixing ring 115. The insert block 113 cooperates with the groove on the mounting plate 114. There are multiple insert blocks 113 and they are evenly distributed. Each insert block 113 is rotatably connected to a sliding column 112 respectively. The sliding column 112 is slidably installed on the safety swivel 110. The safety swivel 110 rotates around the axis. The safety swivel 110 is rotatably connected to the fixing ring 115, and the fixing ring 115 is fixedly installed on the support 102.

[0027] The safety swivel 110 is coaxially fixedly connected with the large gear ring 111, which is rotatably mounted on the support 102. The large gear ring 111 is meshed with the safety gear 118, which is rotatably mounted on the support 102. The safety gear 118 is coaxially fixedly connected with the knob 105, which is rotatably mounted on the housing.

[0028] A liquid inlet mechanism 2 is installed on the protective cover 117, and the liquid inlet mechanism 2 includes a pressure rod 201, a reset spring 202, a spiral rod 203, a matching cylinder 204, a mounting rod 205, a rotating frame 206, an upper guide plate 207, a sliding rod 208, a lower guide plate 209, a sliding piece 210, and a dropper 211; the matching cylinder 204 is fixedly installed on the protective cover 117, and a sliding block is arranged on the matching cylinder 204, and the sliding block on the matching cylinder 204 is slidably installed in the spiral groove of the spiral rod 203, and a reset spring 202 is sleeved on the spiral rod 203, and the first end of the reset spring 202 is connected to the spiral rod 203, and the second end of the reset spring 202 is connected to the matching cylinder 204.

[0029] A lower guide plate 209 is fixedly mounted on the protective cover 117 , and a plurality of slides 210 are evenly and slidably mounted on the lower guide plate 209 . Each slide 210 is connected to a slide rod 208 , and the slide rod 208 is slidably mounted on the upper guide plate 207 . The upper guide plate 207 is fixedly connected to the rotating frame 206 .

[0030] The lower guide plate 209 is provided with a material drop opening. Under normal conditions, adjacent slides 210 are attached together to block the material drop opening.

[0031] The rotating frame 206 is rotatably installed in the protective cover 117 , and the rotating frame 206 is slidably matched with the mounting rod 205 , and the mounting rod 205 is fixedly arranged on the spiral rod 203 .

[0032] The first end of the spiral rod 203 is connected to the pressure rod 201 , the pressure rod 201 is slidably mounted on the housing, and the second end of the spiral rod 203 is connected to the drip tube 211 .

[0033] The protective cover 117 is provided with an observation window.

[0034] The working principle of the present invention is as follows: the protection spring 108 is always kept in a compressed state, the centrifugal platform 116 is inserted on the mounting plate 114, and a centrifugal tube that needs to be centrifuged is placed in the centrifugal platform 116, and the position of the centrifugal tube corresponds to the position of the dropper 211. By pressing the pressing rod 201, the spiral rod 203 is pressed down, and the reset spring 202 is compressed. The spiral rod 203 is threadedly matched with the slider on the matching cylinder 204. When the spiral rod 203 moves down, it rotates, driving the mounting rod 205 to rotate, and the mounting rod 205 drives the rotating frame 206 to rotate, driving the upper guide plate 207 to rotate, driving the sliding rod 208 to slide on the upper guide plate 207, and driving the sliding plate 210 to slide on the lower guide plate 209, so that multiple sliding plates 210 are separated to expose the drop port, and the dropper 211 drips embryonic stem cell culture medium into the centrifugal tube. After dripping, the spiral rod 203 is reset under the elastic force of the reset spring 202.

[0035] Under normal conditions, the clamping ring 109 is pressed against the mounting plate 114 under the elastic force of the protection spring 108, so that the mounting plate 114 contacts the rotating shaft 107, and the starting motor 101 drives the driving gear 103 to rotate, thereby driving the large gear 104 to rotate, driving the small gear ring 106 to rotate, and the small gear ring 106 drives the rotating shaft 107 to rotate. The rotating shaft 107 drives the clamping ring 109 and the mounting plate 114 to rotate through the elastic pressure of the protection spring 108, and the mounting plate 114 drives the centrifugal platform 116 to rotate for centrifugation.

[0036] After centrifugation is completed, the knob 105 is manually twisted, and the knob 105 drives the safety gear 118 to rotate, drives the large gear ring 111 to rotate, drives the safety swivel 110 to rotate, drives the sliding column 112 to slide on the safety swivel 110, pushes the plug block 113 to slide on the fixed ring 115, and drives the plug block 113 to be stuck in the groove on the mounting plate 114. At this time, the mounting plate 114 cannot rotate, and the position of the knob 105 is locked.

[0037] The protective cover 117 is lifted, and the centrifuge tube is taken out for subsequent operation. Then, the centrifuge platform 116 is taken out for cleaning and disinfection, and the position of the mounting plate 114 and the centrifuge platform 116 is limited by the insert block 113 inserted into the groove of the mounting plate 114 to avoid the machine from suddenly operating when taking out the centrifuge tube or cleaning the device later, which may affect the safety of the experiment.

[0038] The centrifugal changes in the protective cover 117 can be observed through the observation window.

[0039] It should be understood that the above embodiments are only used to illustrate the technical solutions of the present invention rather than to limit it. For those skilled in the art, the technical solutions described in the above embodiments can be modified, or some of the technical features therein can be replaced by equivalents; and all these modifications and replacements should fall within the scope of protection of the claims attached to the present invention.

Claims

1. A bionic embryonic stem cell culture device, comprising a centrifugal mechanism (1) installed in a housing and a liquid inlet mechanism (2) installed on the centrifugal mechanism (1), characterized in that: The centrifugal mechanism (1) comprises a safety component and a support (102). A rotating shaft (107) is rotatably mounted on the support (102). A protective spring (108) is sleeved on the rotating shaft (107). The first end of the protective spring (108) is connected to the rotating shaft (107). The second end of the protective spring (108) is connected to a clamping ring (109). The clamping ring (109) is slidably mounted on the rotating shaft (107). The rotating shaft (107) is rotatably connected to a mounting plate (114). A centrifugal platform (116) is inserted on the mounting plate (114). The safety component comprises a safety component and a support (102). A fixing ring (115) rotatably connected to the mounting plate (114), the fixing ring (115) being connected to the protective cover (117), an insert block (113) being slidably mounted on the fixing ring (115), the insert block (113) being matched with a groove on the mounting plate (114), a plurality of insert blocks (113) being evenly distributed, each insert block (113) being rotatably connected to a sliding column (112), the sliding column (112) being slidably mounted on a safety swivel (110), the safety swivel (110) being rotatably connected to the fixing ring (115), the safety swivel (110) being rotatably mounted around an axis, and the safety swivel (110) being rotatably connected to the fixing ring (115); The safety swivel (110) is coaxially fixedly connected to the large gear ring (111), the large gear ring (111) is meshed with the safety gear (118), the safety gear (118) is coaxially fixedly connected to the knob (105), and the knob (105) is rotatably mounted on the housing; The protective cover (117) is provided with a liquid inlet mechanism (2), the liquid inlet mechanism (2) comprising a matching cylinder (204) fixedly mounted on the protective cover (117), a slider being provided on the matching cylinder (204), the slider on the matching cylinder (204) being slidably mounted in a spiral groove of a spiral rod (203), a return spring (202) being sleeved on the spiral rod (203), a first end of the return spring (202) being connected to the spiral rod (203), and a second end of the return spring (202) being connected to the matching cylinder (204); A lower guide plate (209) is fixedly mounted on the protective cover (117), a plurality of slide plates (210) are evenly and slidably mounted on the lower guide plate (209), each slide plate (210) is respectively connected to a slide rod (208), the slide rod (208) is slidably mounted on the upper guide plate (207), and the upper guide plate (207) is fixedly connected to the rotating frame (206); The lower guide plate (209) is provided with a material drop opening. Under normal conditions, adjacent slides (210) are attached together to block the material drop opening. The rotating frame (206) is rotatably mounted in the protective cover (117), the rotating frame (206) is slidably matched with the mounting rod (205), and the mounting rod (205) is fixedly mounted on the spiral rod (203); The first end of the spiral rod (203) is connected to the pressure rod (201), and the second end of the spiral rod (203) is connected to the drip tube (211); The protection spring (108) is always kept in a compressed state. The centrifugal platform (116) is inserted on the mounting plate (114). A centrifugal tube to be centrifuged is placed in the centrifugal platform (116). The position of the centrifugal tube corresponds to the position of the dropper (211). By pressing the pressing rod (201), the spiral rod (203) is pressed down, the return spring (202) is compressed, and the spiral rod (203) is matched with the slider thread on the matching cylinder (204). When the spiral rod (203) moves downward, it rotates, driving the mounting cylinder (204) to rotate. The rod (205) rotates, the mounting rod (205) drives the rotating frame (206) to rotate, drives the upper guide plate (207) to rotate, drives the sliding rod (208) to slide on the upper guide plate (207), drives the sliding plate (210) to slide on the lower guide plate (209), so that the plurality of sliding plates (210) are separated to expose the material drop opening, and the dropper (211) drips embryonic stem cell culture medium into the centrifuge tube. After dripping, the spiral rod (203) is reset under the elastic force of the reset spring (202); Under normal conditions, the clamping ring (109) is pressed against the mounting plate (114) by the elastic force of the protection spring (108), so that the mounting plate (114) and the rotating shaft (107) are in contact, and the starting motor (101) drives the driving gear (103) to rotate, thereby driving the large gear (104) to rotate, driving the small gear ring (106) to rotate, and the small gear ring (106) drives the rotating shaft (107) to rotate. The rotating shaft (107) drives the clamping ring (109) and the mounting plate (114) to rotate through the elastic pressure of the protection spring (108), and the mounting plate (114) drives the centrifugal platform (116) to rotate, thereby performing centrifugation; After the centrifugation is finished, the knob (105) is manually twisted, and the knob (105) drives the safety gear (118) to rotate, drives the large gear ring (111) to rotate, drives the safety swivel (110) to rotate, drives the sliding column (112) to slide on the safety swivel (110), pushes the plug block (113) to slide on the fixed ring (115), drives the plug block (113) to be stuck in the groove on the mounting plate (114), and then the mounting plate (114) cannot rotate, and the position of the knob (105) is locked; The protective cover (117) is lifted, and the centrifuge tube is taken out for subsequent operations, and then the centrifuge platform (116) is taken out for cleaning and disinfection, and the position of the mounting plate (114) and the centrifuge platform (116) is limited by inserting the block (113) into the groove of the mounting plate (114).

2. The embryonic stem cell bionic culture device according to claim 1, characterized in that: The protective cover (117) is provided with an observation window.

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