Method for producing refined horse oil

Abstract

The application discloses a preparation method of refined horse oil, and comprises the following steps: step one, cutting horse fat into pieces and placing the horse fat into a reaction container, then adding water, protease and a reaction promoter into the reaction container in sequence, stirring uniformly and sealing; step two, heating the mixture in step one in a constant-temperature water bath; step three, heating and deactivating the enzyme of the mixture treated in step two, then centrifuging to obtain upper oil and grease, and the upper oil and grease is the crude horse oil; step four, adding phosphoric acid into the crude horse oil in step three to prepare degummed horse oil; step five, adding lye into the degummed horse oil in step four to prepare deacidified horse oil; step six, adding pretreated adsorption resin into the deacidified horse oil in step five, fully reacting after constant-temperature water bath shaking, filtering the adsorption resin, soaking and washing the adsorption resin with petroleum ether twice, filtering and collecting the washing liquid, distilling and separating petroleum ether, mixing the remaining liquid with the deacidified horse oil adsorbed by the adsorption resin to obtain the refined horse oil. The refined horse oil prepared by the method has good quality and high safety.

Description

Technical Field

[0001] This invention relates to the field of animal fat processing technology, specifically to a method for preparing refined horse oil. Background Technology

[0002] Horses are an important part of my country's animal husbandry. In 2021, my country's horse population reached 3.672 million, mainly distributed in Xinjiang, Sichuan, and Inner Mongolia. In recent years, the modern horse industry has gradually emerged and shown a good development trend, with the consumer base for horse meat and its products also showing a rapid growth trend. Horse fat is one of the main by-products produced during the slaughtering and processing of horses. Previous studies have shown that the crude fat content in horse fat is about 91%, and compared with other animal fats, its unsaturated fatty acid content is higher, indicating huge potential processing and utilization value. Horse oil products prepared from horse fat through extraction and refining processes have good antioxidant properties and strong permeability, and are widely used in medicine and cosmetics. In recent years, horse oil products have increasingly appeared in our lives, such as horse oil soap, horse oil shampoo, and horse oil face cream. Therefore, designing a method for preparing horse oil from horse fat is of great significance. Summary of the Invention

[0003] This invention provides a method for preparing refined horse oil, which improves the enzymatic hydrolysis effect by adding a special reaction promoter during the enzymatic hydrolysis of horse fat, thereby increasing the extraction rate of crude horse oil. The crude horse oil is then refined by phosphoric acid degumming, alkali deacidification, and decolorization through pre-treated adsorption resin to obtain high-quality refined horse oil.

[0004] To achieve these and other advantages according to the present invention, a method for preparing refined horse oil is provided, comprising the following steps:

[0005] Step 1: Cut the horse fat into pieces no larger than 1cm in volume. 3 The block was placed in the reaction vessel, and then water, protease and reaction promoter were added in sequence. After stirring evenly, the mixture was sealed.

[0006] Step 2: Heat the mixture from Step 1 in a constant temperature water bath at 40-60℃ for 1-3 hours;

[0007] Step 3: After heating the mixture obtained in Step 2 to inactivate the enzymes, centrifuge it and take the upper layer of oil to obtain crude horse oil.

[0008] Step 4: Add phosphoric acid to the crude horse oil from Step 3, and shake and swirl in a constant temperature water bath at 55-65℃ for 8-15 minutes to allow the reaction to proceed. Then centrifuge and collect the supernatant to obtain degummed horse oil.

[0009] Step 5: Add alkaline solution to the degummed horse oil in Step 4, and shake and swirl in a constant temperature water bath at 55-65℃ for 25-35 minutes to react fully. Centrifuge and collect the supernatant to obtain the initial deacidified oil. Add 8-12% by weight of water at 50-60℃ to the initial deacidified oil and stir evenly. Centrifuge and collect the supernatant A. Add 8-12% by weight of water at 50-60℃ to the supernatant A and stir evenly. Centrifuge and collect the supernatant B to obtain the deacidified horse oil.

[0010] Step Six: Add at least twice the volume of pretreated adsorption resin to the deacidified horse oil from Step Five. After fully reacting by shaking in a constant temperature water bath at 55-65°C, filter the adsorption resin and soak and wash it twice with petroleum ether. Filter and collect the combined washing liquid, distill to separate the petroleum ether, and then mix the remaining liquid with the deacidified horse oil adsorbed by the adsorption resin to obtain refined horse oil.

[0011] Preferably, in the method for preparing refined horse oil, the protease is one of neutral protease, alkaline protease, and trypsin.

[0012] Preferably, in the method for preparing refined horse oil, step one involves adding 1 to 5 times the volume of water (by weight of horse fat), 0.5% to 3% of the weight of protease (by weight of horse fat), and 0.3 to 0.8 times the volume of reaction accelerator (by weight of horse fat).

[0013] Preferably, in the method for preparing refined horse oil, the reaction promoter is a mixture of vitamin C, sugar alcohol, sodium thiosulfate, and water in a mass ratio of 1-3:4-8:0.5-1:1500-2000.

[0014] Preferably, in the method for preparing refined horse oil, the reaction promoter is prepared by adding sodium thiosulfate to water, stirring to dissolve, then passing electricity for 100-150 seconds, then adding vitamin C and sugar alcohol, stirring to dissolve, and reacting fully for 5-10 minutes.

[0015] Preferably, in the method for preparing refined horse oil, in step four, the phosphoric acid concentration is 30-50%, and the amount added is 1-3% of the weight of crude horse oil.

[0016] Preferably, in the method for preparing refined horse oil, in step five, the alkaline solution is an aqueous solution of sodium hydroxide or an aqueous solution of potassium hydroxide.

[0017] Preferably, in the method for preparing refined horse oil, the concentration of the alkali solution is 4 mol / L, and the amount added is 0.3-1% of the weight of the degummed horse oil.

[0018] Preferably, in the method for preparing refined horse oil, the pretreatment method of the adsorption resin in step six is ​​as follows: add water of no less than twice its volume to the adsorption resin, heat to boiling, add vitamin C and organic weak acid, boil for 5-10 minutes, cool to room temperature and stand for 5-10 minutes, and filter to obtain the pretreated adsorption resin.

[0019] Preferably, in the method for preparing refined horse oil, the organic weak acid is one of acetic acid, malic acid, and citric acid.

[0020] This invention offers at least the following beneficial effects: It extracts horse oil from horse fat through enzymatic hydrolysis, and adds a specially formulated reaction promoter during the extraction process to accelerate the enzymatic reaction, thereby more efficiently decomposing the horse fat and resulting in more complete horse oil extraction; it pre-treats the adsorption resin to alter its adsorption properties, enhancing its adsorption of impurities, pigments, and heavy metals in the horse oil, thus purifying and refining the horse oil and significantly improving its quality; the horse oil prepared by this invention has higher purity and greater safety.

[0021] Other advantages, objectives and features of the present invention will become apparent in part from the following description, and in part from those skilled in the art through study and practice of the invention. Detailed Implementation

[0022] The present invention will now be described in further detail with reference to specific embodiments, so that those skilled in the art can implement it based on the description.

[0023] It should be understood that terms such as “having,” “comprising,” and “including” as used herein do not exclude the presence or addition of one or more other elements or combinations thereof.

[0024] It should be noted that, unless otherwise specified, the experimental methods described in the following embodiments are conventional methods, and the reagents and materials mentioned are commercially available. In the description of this invention, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "setting" should be interpreted broadly. For example, they can refer to fixed connection or setting, detachable connection or setting, or integral connection or setting. Those skilled in the art can understand the specific meaning of the above terms in this invention based on the specific circumstances. The terms "lateral," "longitudinal," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," and "outer," etc., indicate orientation or positional relationships based on orientation or positional relationships, and are only for the convenience of describing this invention and simplifying the description. They do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this invention.

[0025] Example 1

[0026] Methods for preparing refined horse oil include:

[0027] Step 1: Cut the horse fat into pieces no larger than 1cm in volume. 3 The block of horse fat was placed in a reaction vessel, and then water of 1 volume of horse fat, alkaline protease of 0.5% horse fat weight, and reaction promoter of 0.3 volume of horse fat were added in sequence. After stirring evenly, the mixture was sealed.

[0028] The preparation method of the reaction promoter is as follows: vitamin C, sugar alcohol, sodium thiosulfate and water are taken in a weight ratio of 1:4:0.5:1500. Sodium thiosulfate is added to water and stirred to dissolve. After stirring, electricity is applied for 100s. Then vitamin C and sugar alcohol are added, stirred to dissolve and reacted fully for 5min.

[0029] Step 2: Heat the mixture from Step 1 in a constant temperature water bath at 40°C for 1 hour;

[0030] Step 3: Heat the mixture obtained in Step 2 to 80℃ to inactivate the enzyme for 10 minutes, then centrifuge at 8500 r / min for 15 minutes, and take the upper layer of oil to obtain crude horse oil.

[0031] Step 4: Add 1% by weight of 30% phosphoric acid to the crude horse oil from Step 3, shake and swirl in a 55°C constant temperature water bath for 8 minutes to allow it to react fully, then centrifuge at 6500 r / min for 10 minutes to obtain the supernatant oil for degumming.

[0032] Step 5: Add 0.3% by weight of a 4mol / L sodium hydroxide aqueous solution to the degummed horse oil from Step 4. After shaking and oscillating in a 55°C constant temperature water bath for 25 minutes, centrifuge at 6500 r / min for 10 minutes to obtain the supernatant oil liquid as the initial deacidified oil liquid. Then add 8% by weight of 50°C water to the initial deacidified oil liquid and stir evenly. Centrifuge at 6500 r / min for 10 minutes to obtain the supernatant oil liquid A. Then add 8% by weight of 50°C water to the supernatant oil liquid A and stir evenly. Centrifuge at 6500 r / min for 10 minutes to obtain the supernatant oil liquid B, which is the deacidified horse oil.

[0033] Step 6: Add twice the volume of pretreated adsorption resin to the deacidified horse oil from step 5. After fully reacting by shaking in a constant temperature water bath at 55°C, filter the adsorption resin and soak and wash the adsorption resin twice with petroleum ether. After filtering and collecting the combined washing liquid, distill to separate the petroleum ether and mix the remaining liquid with the deacidified horse oil adsorbed by the adsorption resin to obtain refined horse oil.

[0034] The pretreatment method for the adsorption resin is as follows: add twice the volume of water to the adsorption resin, heat to boiling, add vitamin C and acetic acid, boil for 5 minutes, cool to room temperature and stand for 5 minutes, and then filter to obtain the pretreated adsorption resin.

[0035] Example 2

[0036] Methods for preparing refined horse oil include:

[0037] Step 1: Cut the horse fat into pieces no larger than 1cm in volume. 3 The block of horse fat was placed in a reaction vessel, and then water with a volume of 3 times that of horse fat, neutral protease with a weight of 2% of horse fat, and reaction promoter with a volume of 0.6 times that of horse fat were added in sequence. After stirring evenly, the mixture was sealed.

[0038] The preparation method of the reaction promoter is as follows: vitamin C, sugar alcohol, sodium thiosulfate and water are taken in a weight ratio of 2:6:0.7:1750. Sodium thiosulfate is added to water and stirred to dissolve. After electrolysis for 125s, vitamin C and sugar alcohol are added, stirred to dissolve and reacted fully for 8min.

[0039] Step 2: Heat the mixture from Step 1 in a constant temperature water bath at 50°C for 2 hours;

[0040] Step 3: Heat the mixture obtained in Step 2 to 90℃ to inactivate the enzyme for 13 minutes, then centrifuge at 8500 r / min for 18 minutes, and take the upper layer of oil to obtain crude horse oil.

[0041] Step 4: Add 2% by weight of 40% phosphoric acid to the crude horse oil from Step 3, shake and swirl in a 60°C constant temperature water bath for 11 minutes to react fully, then centrifuge at 6500 r / min for 13 minutes and take the supernatant oil to obtain degummed horse oil.

[0042] Step 5: Add 0.7% by weight of a 4mol / L sodium hydroxide aqueous solution to the degummed horse oil from Step 4. After shaking and agitating in a 60°C constant temperature water bath for 30 minutes, centrifuge at 6500 r / min for 13 minutes to obtain the supernatant oil liquid as the initial deacidified oil liquid. Then add 10% by weight of 55°C water to the initial deacidified oil liquid and stir evenly. Centrifuge at 6500 r / min for 13 minutes to obtain the supernatant oil liquid A. Then add 10% by weight of 55°C water to the supernatant oil liquid A and stir evenly. Centrifuge at 6500 r / min for 13 minutes to obtain the supernatant oil liquid B, which is the deacidified horse oil.

[0043] Step 6: Add three times the volume of the pretreated adsorption resin to the deacidified horse oil from step 5. After fully reacting by shaking in a 60°C constant temperature water bath, filter the adsorption resin and soak and wash it twice with petroleum ether. Filter and collect the combined washing liquid, distill to separate the petroleum ether, and mix the remaining liquid with the deacidified horse oil adsorbed by the adsorption resin to obtain refined horse oil.

[0044] The pretreatment method for the adsorption resin is as follows: add three times the volume of water to the adsorption resin, heat to boiling, add vitamin C and citric acid, boil for 8 minutes, cool to room temperature and stand for 8 minutes, and then filter to obtain the pretreated adsorption resin.

[0045] Example 3

[0046] Methods for preparing refined horse oil include:

[0047] Step 1: Cut the horse fat into pieces no larger than 1cm in volume. 3 The block was placed in a reaction vessel, and then water at 5 times the volume of horse fat, trypsin at 3% of the weight of horse fat, and reaction promoter at 0.8 times the volume of horse fat were added in sequence. After stirring evenly, the mixture was sealed.

[0048] The preparation method of the reaction promoter is as follows: vitamin C, sugar alcohol, sodium thiosulfate and water are taken in a weight ratio of 3:8:1:2000. Sodium thiosulfate is added to water and stirred to dissolve. After stirring and dissolving, electricity is applied for 150s. Then vitamin C and sugar alcohol are added, stirred to dissolve and reacted fully for 10min.

[0049] Step 2: Heat the mixture from Step 1 in a constant temperature water bath at 60°C for 3 hours;

[0050] Step 3: Heat the mixture obtained in Step 2 to 100℃ to inactivate the enzyme for 15 minutes, then centrifuge at 8500 r / min for 20 minutes, and take the upper layer of oil to obtain crude horse oil.

[0051] Step 4: Add 3% by weight of 50% phosphoric acid to the crude horse oil from Step 3, shake and swirl in a 65°C constant temperature water bath for 15 minutes to allow it to react fully, then centrifuge at 6500 r / min for 15 minutes and take the supernatant to obtain degummed horse oil.

[0052] Step 5: Add 1% by weight of a 4mol / L potassium hydroxide aqueous solution to the degummed horse oil from Step 4. After shaking and agitating in a 65°C constant temperature water bath for 35 minutes, centrifuge at 6500 r / min for 15 minutes to obtain the supernatant oil liquid as the initial deacidified oil liquid. Then add 12% by weight of 60°C water to the initial deacidified oil liquid and stir evenly. Centrifuge at 6500 r / min for 15 minutes to obtain the supernatant oil liquid A. Then add 12% by weight of 60°C water to the supernatant oil liquid A and stir evenly. Centrifuge at 6500 r / min for 15 minutes to obtain the supernatant oil liquid B, which is the deacidified horse oil.

[0053] Step 6: Add 5 times the volume of pretreated adsorption resin to the deacidified horse oil from step 5. After fully reacting by shaking in a constant temperature water bath at 65°C, filter the adsorption resin and soak and wash the adsorption resin twice with petroleum ether. After filtering and collecting the combined washing liquid, distill to separate the petroleum ether and mix the remaining liquid with the deacidified horse oil adsorbed by the adsorption resin to obtain refined horse oil.

[0054] The pretreatment method for the adsorption resin is as follows: add 5 times the volume of water to the adsorption resin, heat to boiling, add vitamin C and malic acid, boil for 10 minutes, cool to room temperature and stand for 10 minutes, and filter to obtain the pretreated adsorption resin.

[0055] Comparative Example 1

[0056] Same as Example 2, except that no reaction promoter was added in step one.

[0057] Comparative Example 2

[0058] Same as Example 2, except that in step one, there is no energizing step in the preparation method of the reaction promoter.

[0059] Comparative Example 3

[0060] Same as Example 2, except that in step one, vitamin C and sugar alcohol are not added in the preparation method of the reaction promoter.

[0061] Comparative Example 4

[0062] Same as Example 2, except that in step one, sodium thiosulfate and sugar alcohol are not added in the preparation method of the reaction promoter.

[0063] Comparative Example 5

[0064] Same as Example 2, except that in step one, sodium thiosulfate and vitamin C are not added in the preparation method of the reaction promoter.

[0065] Comparative Example 6

[0066] Same as Example 2, except that in step one, vitamin C is not added in the preparation method of the reaction promoter.

[0067] Comparative Example 7

[0068] Same as Example 2, except that in step one, sugar alcohol is not added in the preparation method of the reaction promoter.

[0069] Comparative Example 8

[0070] Same as Example 2, except that in step one, sodium thiosulfate is not added in the preparation method of the reaction promoter, and no electricity is applied.

[0071] Comparative Example 9

[0072] Same as Example 2, except that sodium thiosulfate is not added in step one of the reaction accelerator preparation method.

[0073] Comparative Example 10

[0074] Same as Example 2, except that in step one, sodium thiosulfate, vitamin C, and sugar alcohol are added to water first, and then electricity is applied.

[0075] Comparative Example 11

[0076] Dry extraction of crude horse oil: Cut horse fat into pieces no larger than 1 cm in volume. 3 The block was placed in a reaction vessel, sealed, and then placed in a 95°C water bath for 2 hours. After centrifugation at 8500 r / min for 15 minutes, the upper liquid was taken as crude horse oil.

[0077] Comparative Example 12

[0078] Same as Example 2, except that the adsorption resin in step six was not pretreated.

[0079] Comparative Example 13

[0080] Same as Example 2, except that vitamin C and citric acid are not added in the pretreatment method of the adsorption resin in step six.

[0081] Comparative Example 14

[0082] Same as Example 2, except that: in step six, the pretreatment method of the adsorption resin does not involve heating or boiling, and citric acid is not added.

[0083] Comparative Example 15

[0084] Same as Example 2, except that: in step six, the pretreatment method of the adsorption resin does not involve heating or boiling, and no vitamin C is added.

[0085] Comparative Example 16

[0086] Same as Example 2, except that citric acid is not added in the pretreatment method of the adsorption resin in step six.

[0087] Comparative Example 17

[0088] Same as Example 2, except that vitamin C is not added in the pretreatment method of the adsorption resin in step six.

[0089] Comparative Example 18

[0090] Same as Example 2, except that there is no heating and boiling operation in the pretreatment method of the adsorption resin in step six.

[0091] Comparative Example 19

[0092] Same as Example 2, except that in step six, appropriate activated clay is added to the degummed horse oil in step five, and the mixture is shaken in a constant temperature water bath at 60°C until the reaction is complete. The supernatant is then centrifuged to obtain refined horse oil.

[0093] In the above examples and comparative examples, the enzyme activity of the protease was 200,000 U / g.

[0094] The extraction rates of crude horse oil in Examples 1-3 and Comparative Examples 1-11 were measured and calculated, and the results were recorded in Table 1. The recovery rates of crude horse oil in Examples 1-3 and Comparative Examples 12-19 were measured and calculated. The acid value, peroxide value, iodine value, yellowness value, saponification value, chromium content, and lead content of refined horse oil in Examples 1-3 and Comparative Examples 12-19 were measured, and the results were recorded in Table 2. The acid value, peroxide value, iodine value, yellowness value, saponification value, chromium content, and lead content of crude horse oil in Example 2 were measured, and the results were recorded in Table 3. The crude horse oil extraction rate = (mass of crude horse oil / mass of horse fat raw material) × 100%, and the crude horse oil recovery rate = (mass of refined horse oil / mass of crude horse oil) × 100%.

[0095] Table 1

[0096]

[0097] Table 2

[0098]

[0099] Table 3

[0100]

[0101] Analysis of the test results in Tables 1, 2, and 3 shows that compared to the conventional dry-processing of crude horse oil (Comparative Example 11), the extraction rate of crude horse oil obtained by the method of this invention (Examples 1-3) is significantly improved, and the recovery rate of horse oil after degumming, deacidification, and decolorization can reach as high as 93.41%, indicating that the method of this invention can fully extract horse oil from horse fat with low impurity content. The method of this invention adds a specially formulated reaction promoter during the enzymatic hydrolysis of horse fat to accelerate the degree of enzymatic hydrolysis, achieving the purpose of fully extracting horse oil. After degumming, deacidification, and decolorization refining of crude horse oil using the method of this invention (Examples 1-3), compared to crude horse oil, the yellowness value, acid value, peroxide value, saponification value, chromium content, and lead content are significantly reduced, while the iodine value increases, indicating that the quality of horse oil obtained after refining by the method of this invention is significantly improved. This invention, by pretreating the adsorption resin, changes its adsorption performance, enhancing its adsorption of impurities, pigments, and heavy metals such as chromium and lead, effectively improving the quality of horse oil.

[0102] The number of devices and processing scale described herein are for the purpose of simplifying the description of the invention. Applications, modifications, and variations of the invention will be readily apparent to those skilled in the art.

[0103] Although embodiments of the present invention have been disclosed above, they are not limited to the applications listed in the specification and embodiments. They can be applied to various fields suitable for the present invention. For those skilled in the art, other modifications can be easily made. Therefore, without departing from the general concept defined by the claims and their equivalents, the present invention is not limited to the specific details and embodiments shown and described herein.

Claims

1. A method for preparing refined horse oil, characterized in that, Includes the following steps: Step 1: Cut the horse fat into pieces no larger than 1cm in volume. 3 The block was placed in the reaction vessel, and then water, protease and reaction promoter were added in sequence. After stirring evenly, the mixture was sealed. Step 2: Heat the mixture from Step 1 in a constant temperature water bath at 40~60℃ for 1~3 hours; Step 3: After heating the mixture obtained in Step 2 to inactivate the enzymes, centrifuge it and take the upper layer of oil to obtain crude horse oil. Step 4: Add phosphoric acid to the crude horse oil from Step 3, and shake and swirl in a constant temperature water bath at 55-65℃ for 8-15 minutes to allow the reaction to proceed. Then centrifuge and collect the supernatant to obtain degummed horse oil. Step 5: Add alkaline solution to the degummed horse oil in Step 4, and shake and vibrate in a constant temperature water bath at 55~65℃ for 25~35 minutes to react fully. Centrifuge and take the supernatant to obtain the initial deacidified oil. Add 8~12% by weight of water at 50~60℃ to the initial deacidified oil and stir evenly. Centrifuge and take the supernatant A. Add 8~12% by weight of water at 50~60℃ to the supernatant A and stir evenly. Centrifuge and take the supernatant B to obtain the deacidified horse oil. Step 6: Add at least twice the volume of pretreated adsorption resin to the deacidified horse oil from step 5. After fully reacting by shaking in a constant temperature water bath at 55~65℃, filter the adsorption resin and soak and wash the adsorption resin twice with petroleum ether. After filtering and collecting the combined washing liquid, distill to separate the petroleum ether and mix the remaining liquid with the deacidified horse oil adsorbed by the adsorption resin to obtain refined horse oil. The reaction promoter is a mixture of vitamin C, sugar alcohol, sodium thiosulfate, and water in a mass ratio of 1~3:4~8:0.5~1:1500~2000; the reaction promoter is prepared by adding sodium thiosulfate to water, stirring and dissolving, then passing electricity for 100~150s, then adding vitamin C and sugar alcohol, stirring and dissolving, and reacting fully for 5~10min.

2. The method for preparing refined horse oil as described in claim 1, characterized in that, The protease is one of neutral protease, alkaline protease, or trypsin.

3. The method for preparing refined horse oil as described in claim 1, characterized in that, In step one, add 1 to 5 times the volume of horse fat in water, 0.5% to 3% of the weight of horse fat in protease, and 0.3 to 0.8 times the volume of horse fat in a reaction accelerator.

4. The method for preparing refined horse oil as described in claim 1, characterized in that, In step four, the phosphoric acid concentration is 30-50%, and the amount added is 1-3% of the weight of crude horse oil.

5. The method for preparing refined horse oil as described in claim 1, characterized in that, In step five, the alkaline solution is an aqueous solution of sodium hydroxide or potassium hydroxide.

6. The method for preparing refined horse oil as described in claim 1, characterized in that, The concentration of the alkali solution is 4 mol / L, and the amount added is 0.3~1% of the weight of the degummed horse oil.

7. The method for preparing refined horse oil as described in claim 1, characterized in that, The pretreatment method for the adsorption resin in step six is ​​as follows: add water of no less than twice its volume to the adsorption resin, heat to boiling, add vitamin C and organic weak acid, boil for 5-10 minutes, cool to room temperature and stand for 5-10 minutes, and filter to obtain the pretreated adsorption resin.

8. The method for preparing refined horse oil as described in claim 7, characterized in that, The organic weak acid is one of acetic acid, malic acid, and citric acid.

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