Feed additive and use thereof

Abstract

The application discloses a kind of feed additive and its application, it is related to feed additive technical field.The prepared feed additive of the application is by mixing sodium glucuronate, glucomannan, dimethyl cyclodextrin, diatomite and sodium lauryl sulfate, then mixed solution is reacted with calcium chloride crosslinking solution, finally form microspheres type feed additive.Compared with prior art, the prepared feed additive of the application is beneficial to the adsorption of deoxynivalenol and zearalenone, amino acid liquid, lemon yellow and titanium dioxide are mixed, then composite bacteria liquid is added to ferment, by feed additive processing, low-temperature drying can retain the nutrient components and biological activity in raw materials, and the biological fermentation protein raw material with good sensory color is obtained.

Description

Technical Field

[0001] This invention relates to the field of feed additive technology, and in particular to a feed additive and its application. Background Technology

[0002] The treatment of threonine mother liquor has always been a hot topic. Several existing technologies exist for treating threonine mother liquor. One commonly used method is ion exchange column separation, which uses ion exchange columns to separate impurities from threonine in the mother liquor, achieving a high threonine recovery rate and providing decolorization and desaccharification effects. However, this method involves expensive equipment and high operating costs, making it suitable for small-scale production. Membrane separation technology utilizes the separation properties of membranes to separate pigments, sugars, and threonine from the threonine mother liquor, offering advantages such as convenient operation, simple equipment, and energy efficiency. However, membrane materials are prone to fouling and damage, requiring regular backwashing, cleaning, and inspection, resulting in high operating costs. Multi-effect evaporation and spray drying technology treats threonine mother liquor into solid organic compound fertilizer, with wastewater discharged after meeting standards, making it a feasible treatment method. However, this method has relatively low efficiency in large-scale production. Chromatographic separation technology effectively separates pigments, sugars, and threonine from the threonine mother liquor, improving the threonine recovery rate and reducing production losses. However, this method is not currently widely used in production due to operational instability. Electrodialysis desalination technology explores optimal conditions for desalination by altering operating conditions such as desalination solution flow rate and electrodialysis current density, providing a feasible method for treating threonine mother liquor. Hydrolysis to reduce sugar concentration converts polysaccharides in threonine mother liquor into monosaccharides through pH adjustment with sulfuric acid followed by heating and hydrolysis, improving sugar utilization, reducing residual sugar content, decreasing production costs and energy consumption, reducing pollutant emissions, and protecting the environment.

[0003] The above technologies have been applied and researched in the treatment of feed additives in threonine mother liquor, aiming to improve product quality, reduce costs, and reduce environmental pollution.

[0004] This invention utilizes a method of re-fermentation of threonine mother liquor. Currently, the feed additive catalog contains many colorants, such as: tartrazine, sunset yellow, allura red, carmine, indigo, titanium dioxide, caramel color (ammonium sulfite method), erythrosine, etc. After mixing these colorants in water according to a specific ratio, the mixture is sprayed onto other raw material mixtures and mixed thoroughly to form a feed product with a bright yellow sensory color. However, the color of feed products produced using this method is prone to change under different temperature and humidity conditions, resulting in unstable coloring effects.

[0005] This invention optimizes the color of protein raw materials by adjusting the color of amino acid liquid raw materials, thereby increasing the product's cost-effectiveness. Furthermore, through the preparation of feed additives, it achieves the adsorption of deoxynivalenol and zearalenone, resulting in bio-fermented protein raw materials with better palatability and safety.

[0006] Chinese invention patent CN1240828C discloses a microbial fermentation broth for feed, composed of beneficial bacteria and a culture medium. The beneficial bacteria include Bacillus, lactic acid bacteria, yeast, photosynthetic bacteria, and actinomycetes. The culture medium includes amino acid solution, sugar, and water. The fermentation broth contains 2-6 parts beneficial bacteria, 5-15 parts amino acid solution, 2-4 parts sugar, and 330-380 parts water. Its formula is reasonable, and the beneficial bacteria have a good effect on the fermentation of agricultural by-products, with high efficiency. The raw materials are readily available and low in cost. The fermented feed has good palatability, effectively improves the nutritional components of the feed, facilitates digestion and absorption, promotes livestock growth, improves feed utilization, and shortens the feeding period. However, the color of the fermentation broth prepared by this invention is unstable, and it is prone to producing deoxynivalenol and zearalenone, affecting feed safety. Summary of the Invention

[0007] In view of the shortcomings of the existing technology, the technical problem to be solved by the present invention is to provide a feed additive that can adsorb harmful substances and its application.

[0008] To achieve the above-mentioned objectives, the present invention adopts the following technical solution:

[0009] The preparation method of a feed additive is as follows, in parts by weight:

[0010] Add 1-3 parts of a sodium-containing compound to 80-120 parts of water to obtain an aqueous solution. Add 0.5-2 parts of a polymer compound, 0.3-0.8 parts of an adsorbent, and 0.3-0.8 parts of a dispersant to the aqueous solution and mix thoroughly to obtain a mixed solution. Add the above mixed solution to a calcium chloride crosslinking solution at a rate of 0.3-0.8 mL / min. The calcium chloride crosslinking solution is prepared by adding 3-5 parts of calcium chloride and 1-3 parts of chitosan to 80-120 parts of a 1-3 wt% acetic acid aqueous solution. Stir and solidify at room temperature (10-30 rpm) for 20-40 min, then filter, collect the microspheres, and rinse with water to obtain a feed additive.

[0011] The sodium-containing compound is at least one of sodium glucuronate and sodium alginate.

[0012] The polymeric compound is at least one of glucomannan, dimethylcyclodextrin, soluble starch, and β-glucan.

[0013] The adsorbent is at least one of diatomaceous earth and montmorillonite.

[0014] The dispersant is at least one of sodium lauryl sulfate and sodium dodecyl sulfate.

[0015] The feed additives are used as follows, in parts by weight:

[0016] Mix 50-70 parts of amino acid solution, 1-3 parts of lemon yellow, and 0.5-2 parts of titanium dioxide thoroughly for 5-10 minutes using a high-frequency mixing tank. Then add 3-8 parts of compound bacterial solution and mix thoroughly. After solid-state fermentation for 24-72 hours, add 3-5 parts of the feed additives mentioned above and stir at 5-20 rpm for 40-80 minutes at 25-35°C. Then filter through a 150-300 mesh sieve, collect the liquid, and dry the material using a fluidized bed dryer with a low-temperature drying process. After drying, pulverize and pass through a 200-400 mesh sieve to obtain a bio-fermented protein raw material with good sensory color.

[0017] The drying temperature of the low-temperature drying process is controlled at 60~80℃.

[0018] The preparation method of the compound bacterial solution is as follows, in parts by weight:

[0019] Add 15-25 parts glucose and 70-90 parts enrichment agent to water at 70-90℃ and mix thoroughly for 5-15 minutes. After cooling to 30-40℃, add 1.8-5.8 parts compound bacterial powder and activate at 30-40℃ for 2-6 hours to obtain the final product.

[0020] The enrichment agent consists of the following components in parts by weight: 18-20 parts tryptone, 5-8 parts yeast extract, 12-15 parts beef extract, 0.5-1 part disodium hydrogen phosphate, 1-3 parts sodium chloride, and purified water to make up to 1000 parts.

[0021] The compound microbial powder is composed of the following components by weight: 1-3 parts of Lactobacillus plantarum, 0.5-2 parts of Bacillus subtilis, and 0.3-0.8 parts of Saccharomyces cerevisiae.

[0022] The functions of each substance in this invention are as follows:

[0023] Sodium gluconate: Used in the preparation of aqueous solutions, it may play a role in dissolving and stabilizing other components.

[0024] Water: Used as a solvent to prepare aqueous solutions, and plays a role in mixing and dilution in subsequent steps.

[0025] Glucomannan: As one of the components of the mixed solution, it may be used as a building material for microspheres.

[0026] Diatomaceous earth: As one of the components of the mixed solution, it has adsorption properties and may be used as a building material for microspheres.

[0027] Sodium lauryl sulfate: As one of the components of the mixed solution, it has dispersibility.

[0028] Calcium chloride: Used to prepare calcium chloride crosslinking solution, which undergoes ionic crosslinking reaction with chitosan, promoting the formation of microspheres.

[0029] Chitosan: One of the components added to the calcium chloride crosslinking solution, it undergoes an ionic crosslinking reaction with calcium chloride, promoting the formation of microspheres.

[0030] Acetic acid: used as one of the solvents in calcium chloride crosslinking solutions.

[0031] Compound bacterial liquid: contains strains such as Lactobacillus plantarum, Bacillus subtilis and Saccharomyces cerevisiae, which participate in liquid and solid fermentation processes to produce beneficial metabolites.

[0032] The bio-fermented protein raw material prepared by this invention is characterized by good and stable sensory color and excellent palatability.

[0033] Amino acid liquid refers to the tail liquid formed after corn deep processing to produce amino acid products (such as threonine and lysine). It typically has a moisture content of over 50%, high nutritional value, but a very dark color. The amino acid used here is mainly threonine tail liquid, which serves as part of the fermentation substrate, providing nutrients to the microbial inoculum. The application method of this invention can increase the threonine content in raw material colorants and finished feed.

[0034] Both lemon yellow and titanium dioxide are colorants; adding lemon yellow turns the corn yellow, and adding titanium dioxide turns it white. Proper mixing is essential to ensure the color of the colorant closely resembles the color of the corn.

[0035] The material is dried using a low-temperature drying process (fluidized bed drying equipment). The reason for low-temperature drying is to ensure that the dried product has a lighter color and to ensure that more of the metabolites formed during solid-state fermentation are retained.

[0036] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0037] 1) The feed additive prepared according to the present invention: Sodium gluconate, glucomannan, dimethyl cyclodextrin, diatomaceous earth, and sodium lauryl sulfate are mixed, and then the mixed solution is reacted with a calcium chloride crosslinking solution to ultimately form a microsphere-type feed additive. This preparation method allows for microscale control of the feed additive, improves its stability, and facilitates the adsorption of deoxynivalenol and zearalenone.

[0038] 2) Application of the feed additive of this invention: Amino acid solution, lemon yellow and titanium dioxide are mixed, then a compound bacterial solution is added for fermentation, and then the mixture is treated with feed additives. This process promotes the growth and metabolism of the microorganisms, further improving the quality and nutritional value of the feed.

[0039] 3) Preparation of bio-fermented protein raw materials of the present invention: After solid-state fermentation and drying, the raw materials are dried at low temperature by fluidized bed drying equipment, which can retain the nutrients and bioactivity in the raw materials and obtain bio-fermented protein raw materials with good sensory color. Detailed Implementation

[0040] Main source of materials:

[0041] Glucomannan: Shanxi Yirun Biotechnology Co., Ltd., Product No.: 000001.

[0042] Diatomaceous earth: Henan Antong Environmental Protection Technology Co., Ltd., Model: 325.

[0043] Chitosan: Guangzhou Bloomage Biotechnology Co., Ltd., Model: 101.

[0044] Soluble starch: Langfang Qianyao Technology Co., Ltd., Item No.: 0145.

[0045] Amino acid liquid: refers to the tail liquid formed after corn is processed into amino acid products (such as threonine, lysine, etc.). The amino acid liquid used in this invention is the tail liquid generated by Shandong Guangkun Biotechnology Co., Ltd. in the production of amino acid raw powder.

[0046] Titanium dioxide: Shenzhen Xinrongtai Trading Co., Ltd., Model: Food grade.

[0047] Lactobacillus plantarum (CGMCC: 1.16089); Bacillus subtilis (CGMCC: 1.821); Saccharomyces cerevisiae (CGMCC: 2.3973); all provided by Inner Mongolia Agricultural University.

[0048] Montmorillonite: Shandong Youju Chemical Technology Co., Ltd., Item No.: 01. Example 1

[0049] A method for preparing a feed additive is as follows:

[0050] 2g of sodium gluconate was added to 100g of water to obtain an aqueous solution of sodium gluconate. 1g of glucomannan, 0.5g of diatomaceous earth, and 0.5g of sodium lauryl sulfate were added to the sodium gluconate aqueous solution and mixed thoroughly to obtain a mixed solution. The above mixed solution was added to a calcium chloride crosslinking solution at a rate of 0.5mL / min. The calcium chloride crosslinking solution was prepared by adding 4g of calcium chloride and 2g of chitosan to 100g of 2wt% acetic acid aqueous solution. The mixture was stirred and solidified at 20rpm at room temperature for 30min, then filtered, the microspheres were collected, and rinsed with water to obtain a feed additive.

[0051] The feed additive is used as follows, namely, a method for preparing a bio-fermented protein raw material:

[0052] 60g of amino acid solution, 2g of lemon yellow, and 1g of titanium dioxide were thoroughly mixed for 8 minutes using a high-frequency mixing tank. Then, 5g of compound bacterial solution was added and thoroughly mixed. After 48 hours of solid-state fermentation, 4g of feed additive was added, and the mixture was stirred at 10 rpm for 60 minutes at 30°C. The mixture was then filtered through a 200-mesh sieve, and the liquid was collected. The material was then dried using a fluidized bed dryer with a low-temperature drying process, with the drying temperature controlled at 70°C. After drying, the material was pulverized and passed through a 300-mesh sieve to obtain a bio-fermented protein raw material with good sensory color.

[0053] The method for preparing the compound bacterial solution is to add 20g of glucose and 80g of enriching agent to water at 80℃ and mix thoroughly for 10min, cool to 35℃, add 3.5g of compound bacterial powder, and activate at 35℃ for 4h. The enriching agent consists of 20g of tryptone, 6g of yeast extract, 13g of beef extract, 0.8g of disodium hydrogen phosphate, 2g of sodium chloride, and purified water to make up to 1000g. The compound bacterial powder consists of 2g of Lactobacillus plantarum, 1g of Bacillus subtilis, and 0.5g of Saccharomyces cerevisiae. Example 2

[0054] A method for preparing a feed additive is as follows:

[0055] 2g of sodium gluconate was added to 100g of water to obtain an aqueous solution of sodium gluconate. 1g of dimethyl cyclodextrin, 0.5g of diatomaceous earth, and 0.5g of sodium lauryl sulfate were added to the sodium gluconate aqueous solution and mixed thoroughly to obtain a mixed solution. The above mixed solution was added to a calcium chloride crosslinking solution at a rate of 0.5mL / min. The calcium chloride crosslinking solution was prepared by adding 4g of calcium chloride and 2g of chitosan to 100g of 2wt% acetic acid aqueous solution. The mixture was stirred and solidified at 20rpm at room temperature for 30min, then filtered, the microspheres were collected, and rinsed with water to obtain a feed additive.

[0056] The application of the feed additive is the same as in Example 1.

[0057] The preparation method of the compound bacterial solution is the same as that in Example 1. Example 3

[0058] A method for preparing a feed additive is as follows:

[0059] 2g of sodium gluconate was added to 100g of water to obtain an aqueous solution of sodium gluconate. 1g of soluble starch, 0.5g of diatomaceous earth, and 0.5g of sodium lauryl sulfate were then added to the sodium gluconate aqueous solution and mixed thoroughly to obtain a mixed solution. This mixed solution was then added to a calcium chloride crosslinking solution at a rate of 0.5mL / min. The calcium chloride crosslinking solution was prepared by adding 4g of calcium chloride and 2g of chitosan to 100g of a 2wt% acetic acid aqueous solution. The mixture was stirred at 20rpm at room temperature for 30min to solidify, then filtered, the microspheres were collected, and rinsed with water to obtain a feed additive.

[0060] The application of the feed additive is the same as in Example 1.

[0061] The preparation method of the compound bacterial solution is the same as that in Example 1. Example 4

[0062] A method for preparing a feed additive is as follows:

[0063] 2g of sodium gluconate was added to 100g of water to obtain an aqueous solution of sodium gluconate. 0.5g of glucomannan, 0.5g of dimethyl cyclodextrin, 0.5g of diatomaceous earth, and 0.5g of sodium lauryl sulfate were added to the sodium gluconate aqueous solution and mixed thoroughly to obtain a mixed solution. This mixed solution was then added to a calcium chloride crosslinking solution at a rate of 0.5mL / min. The calcium chloride crosslinking solution was prepared by adding 4g of calcium chloride and 2g of chitosan to 100g of a 2wt% acetic acid aqueous solution. The mixture was stirred at 20rpm at room temperature for 30min to solidify, then filtered, the microspheres were collected, and rinsed with water to obtain a feed additive.

[0064] The application of the feed additive is the same as in Example 1.

[0065] The preparation method of the compound bacterial solution is the same as that in Example 1.

[0066] Comparative Example 1

[0067] A method for preparing a feed additive is as follows:

[0068] 2g of sodium alginate was added to 100g of water to obtain an aqueous solution of sodium alginate. 1g of glucomannan, 0.5g of diatomaceous earth, and 0.5g of sodium lauryl sulfate were added to the sodium alginate aqueous solution and mixed thoroughly to obtain a mixed solution. The above mixed solution was added to a calcium chloride crosslinking solution at a rate of 0.5mL / min. The calcium chloride crosslinking solution was prepared by adding 4g of calcium chloride and 2g of chitosan to 100g of 2wt% acetic acid aqueous solution. The mixture was stirred and solidified at 20rpm at room temperature for 30min, then filtered, the microspheres were collected, and rinsed with water to obtain a feed additive.

[0069] The application of the feed additive is the same as in Example 1.

[0070] The preparation method of the compound bacterial solution is the same as that in Example 1.

[0071] Comparative Example 2

[0072] A method for preparing a feed additive is as follows:

[0073] 2g of sodium gluconate was added to 100g of water to obtain an aqueous solution of sodium gluconate. 1g of β-glucan, 0.5g of diatomaceous earth, and 0.5g of sodium lauryl sulfate were added to the sodium gluconate aqueous solution and mixed thoroughly to obtain a mixed solution. The above mixed solution was added to a calcium chloride crosslinking solution at a rate of 0.5mL / min. The calcium chloride crosslinking solution was prepared by adding 4g of calcium chloride and 2g of chitosan to 100g of 2wt% acetic acid aqueous solution. The mixture was stirred and solidified at 20rpm at room temperature for 30min, then filtered, the microspheres were collected, and rinsed with water to obtain a feed additive.

[0074] The application of the feed additive is the same as in Example 1.

[0075] The preparation method of the compound bacterial solution is the same as that in Example 1.

[0076] Comparative Example 3

[0077] A method for preparing a feed additive is as follows:

[0078] 2g of sodium gluconate was added to 100g of water to obtain an aqueous solution of sodium gluconate. 1g of glucomannan, 0.5g of montmorillonite, and 0.5g of sodium lauryl sulfate were added to the sodium gluconate aqueous solution and mixed thoroughly to obtain a mixed solution. The above mixed solution was added to a calcium chloride crosslinking solution at a rate of 0.5mL / min. The calcium chloride crosslinking solution was prepared by adding 4g of calcium chloride and 2g of chitosan to 100g of 2wt% acetic acid aqueous solution. The mixture was stirred and solidified at 20rpm at room temperature for 30min, then filtered, the microspheres were collected, and rinsed with water to obtain a feed additive.

[0079] The application of the feed additive is the same as in Example 1.

[0080] The preparation method of the compound bacterial solution is the same as that in Example 1.

[0081] Comparative Example 4

[0082] A method for preparing a feed additive is as follows:

[0083] 2g of sodium gluconate was added to 100g of water to obtain an aqueous solution of sodium gluconate. 1g of glucomannan, 0.5g of diatomaceous earth, and 0.5g of sodium dodecyl sulfate were added to the sodium gluconate aqueous solution and mixed thoroughly to obtain a mixed solution. The above mixed solution was added to a calcium chloride crosslinking solution at a rate of 0.5mL / min. The calcium chloride crosslinking solution was prepared by adding 4g of calcium chloride and 2g of chitosan to 100g of 2wt% acetic acid aqueous solution. The mixture was stirred and solidified at 20rpm at room temperature for 30min, then filtered, the microspheres were collected, and rinsed with water to obtain a feed additive.

[0084] The application of the feed additive is the same as in Example 1.

[0085] The preparation method of the compound bacterial solution is the same as that in Example 1.

[0086] Comparative Example 5

[0087] A method for preparing a bio-fermented protein raw material is as follows:

[0088] 60g of amino acid solution, 2g of lemon yellow, and 1g of titanium dioxide were thoroughly mixed for 8 minutes using a high-frequency mixing tank. Then, 5g of compound bacterial solution was added and thoroughly mixed. After solid-state fermentation for 48 hours, the mixture was stirred at 10 rpm for 60 minutes at 30°C. The mixture was then filtered through a 200-mesh sieve, and the liquid was collected. The material was then dried using a fluidized bed dryer with a low-temperature drying process, with the drying temperature controlled at 70°C. After drying, the material was pulverized and passed through a 300-mesh sieve to obtain a bio-fermented protein raw material with good sensory color.

[0089] The preparation method of the compound bacterial solution is the same as that in Example 1.

[0090] Test Example 1

[0091] Appearance and color evaluation

[0092] When the bio-fermented protein raw materials prepared in the embodiments and comparative examples are compared with the naked eye under the same field of view, it can be found that the colors of the bio-fermented protein raw materials prepared in the embodiments and comparative examples are similar to the color of corn, stable and bright, and have a certain palatability effect.

[0093] Test Example 2

[0094] Adsorption performance test of deoxynivalenol and zearalenone

[0095] Deoxynivalenol adsorption rate test:

[0096] After adding the feed additives described in the embodiments and comparative examples of this invention, the mixture was filtered through a 200-mesh sieve, and 500 μL of the collected liquid was added to an equal volume of pure methanol. After shaking and mixing, the mixture was centrifuged at 10,000 rpm for 15 min at 5 °C. 500 μL of the supernatant was then collected for HPLC detection. The content of adsorbed deoxynivalenol was determined by HPLC under the following conditions: Column: Agilent 5 TC-C18 (2) reversed-phase column (250 × 4.6 mm, 5 μm); Mobile phase: Methanol:water (15:85); Elution method: isocratic elution; Flow rate: 1 mL / min; Injection volume: 20 μL; Column temperature: 30 °C; UV detector wavelength: 220 nm; Retention time of adsorbed deoxynivalenol: 15 min. Comparative example 5 was used as the control group, and the adsorption rate was calculated according to the following formula.

[0097] W = (V0 - V1) / V0 × 100%

[0098] W represents the adsorption rate of deoxynivalenol; V0 represents the deoxynivalenol content in the control group; and V1 represents the deoxynivalenol content in the experimental group.

[0099] Zearalenone adsorption rate test:

[0100] The feed additives described in the embodiments and comparative examples of this invention were added and filtered through a 200-mesh sieve. 200 μL of the collected liquid was used for high-performance liquid chromatography (HPLC) detection. The sample was eluted with a 60 wt% acetonitrile aqueous solution at a flow rate of 1 mL / min and detected at an absorbance of 254 nm. The adsorption rate was calculated using the following formula.

[0101] X = (T0 - T1) / T0 × 100%

[0102] X represents the adsorption rate of zearalenone; T0 represents the zearalenone content in the control group; and T1 represents the deoxynivalenol content in the experimental group.

[0103] The test results are shown in Table 1.

[0104] Table 1 Adsorption performance test results

[0105] Experimental protocol Deoxynivalenol adsorption rate (%) Adsorption rate (%) of zearalenone Example 1 48.41 71.48 Example 2 47.55 72.14 Example 3 44.73 70.76 Example 4 51.62 75.90 Comparative Example 1 46.75 69.93 Comparative Example 2 45.40 68.39 Comparative Example 3 44.61 68.08 Comparative Example 4 44.82 67.93

[0106] The tests in Examples 1 and 2 show that the feed additives prepared in the embodiments and comparative examples of the present invention have stable and bright colors. The feed additive prepared in Example 4 has a high adsorption rate for deoxynivalenol and zearalenone.

[0107] Glucomannan and dimethylcyclodextrin possess different molecular structures and chemical properties. Prepared as feed additives through reaction, they may enhance the adsorption capacity for mycotoxins through complementary interactions during the adsorption process. For example, the polysaccharide structure of glucomannan may provide more adsorption sites, while the cavity structure of dimethylcyclodextrin may be suitable for the molecular size and shape of mycotoxins. The combination of glucomannan and dimethylcyclodextrin may result in a larger effective adsorption surface area. The cavity structure of dimethylcyclodextrin allows it to encapsulate deoxynivalenol and zearalenone molecules, thereby increasing the effective adsorption surface area and improving adsorption efficiency. Therefore, the combination of glucomannan and dimethylcyclodextrin may enhance the adsorption effect through synergistic effects. They may mutually promote each other during adsorption, forming a more stable composite structure that improves the affinity and adsorption capacity for mycotoxins.

Claims

1. A method for preparing a feed additive, characterized in that, By weight: Add 1-3 parts of a sodium-containing compound to 80-120 parts of water to obtain an aqueous solution. Add 0.5-2 parts of a polymer compound, 0.3-0.8 parts of an adsorbent, and 0.3-0.8 parts of a dispersant to the aqueous solution and mix thoroughly to obtain a mixed solution. Add the above mixed solution to a calcium chloride crosslinking solution at a rate of 0.3-0.8 mL / min. The calcium chloride crosslinking solution is prepared by adding 3-5 parts of calcium chloride and 1-3 parts of chitosan to 80-120 parts of a 1-3 wt% acetic acid aqueous solution. Stir and solidify at room temperature (10-30 rpm) for 20-40 min, then filter, collect the microspheres, and rinse with water to obtain a feed additive. The sodium-containing compound is sodium gluconate; The polymeric compounds are glucomannan and dimethylcyclodextrin; The adsorbent is diatomaceous earth; The dispersant is sodium lauryl sulfate.

2. A feed additive, characterized in that, It is prepared by the preparation method described in claim 1.

3. The application of a feed additive, characterized in that, The application method is as follows, by weight: Mix 50-70 parts of amino acid solution, 1-3 parts of lemon yellow, and 0.5-2 parts of titanium dioxide thoroughly for 5-10 minutes using a high-frequency mixing tank; then add 3-8 parts of compound bacterial solution and mix thoroughly. After 24-72 hours of liquid fermentation, add 3-5 parts of the feed additive described in claim 2, stir at 5-20 rpm for 40-80 minutes at 25-35°C, then filter through a 150-300 mesh sieve, collect the liquid, and dry the material using a fluidized bed dryer with a low-temperature drying process. After drying, pulverize the material through a 200-400 mesh sieve to obtain a bio-fermented protein raw material with good sensory color.

4. The application as described in claim 3, characterized in that, The drying temperature of the low-temperature drying process is controlled at 60~80℃.

5. The application as described in claim 3, characterized in that, The preparation method of the compound bacterial solution is as follows, in parts by weight: Add 15-25 parts glucose and 70-90 parts enrichment agent to water at 70-90℃ and mix thoroughly for 5-15 minutes. After cooling to 30-40℃, add 1.8-5.8 parts compound bacterial powder and activate at 30-40℃ for 2-6 hours to obtain the final product. The enrichment agent is composed of the following components in parts by weight: 18-20 parts tryptone, 5-8 parts yeast extract, 12-15 parts beef extract, 0.5-1 part disodium hydrogen phosphate, 1-3 parts sodium chloride, and purified water to make up to 1000 parts. The compound microbial powder is composed of the following components by weight: 1-3 parts of Lactobacillus plantarum, 0.5-2 parts of Bacillus subtilis, and 0.3-0.8 parts of Saccharomyces cerevisiae.