A rapid antibacterial, low-irritation gynecological lotion and its preparation method

Abstract

The invention provides a rapid antibacterial, low-irritation gynecological lotion and a preparation method thereof. The gynecological lotion comprises the following raw materials in parts by weight: 6-10 parts of water-soluble composite particles, 0.5-0.8 parts of an acid-base regulator, 1-3 parts of a probiotic lysate, 0.6-1.0 parts of water-soluble vitamin E, 12-15 parts of a natural plant extract, and 0.3-0.5 parts of a preservative. The natural plant extract comprises, in parts by weight, 4-6 parts of a toona sinensis bud extract, 4-6 parts of a snow lotus extract, 2-4 parts of a stemona root extract, 1-3 parts of a sophora flavescens extract, 1-3 parts of a phellodendron amurense extract, 1-3 parts of a cnidium monnieri extract, 2-4 parts of a hamamelis virginiana extract, and 2-4 parts of a motherwort extract. The probiotic lysate is prepared by lysing Lactococcus acidophilus, Bifidobacterium bifidum, and Micrococcus longifolia, in a mass ratio of 1.6-1.8:1:0.5-0.8. The gynecological lotion of the present invention can not only achieve the effect of rapid antibacterial effect and eliminate odor, but also reduce irritation to the vaginal skin, thereby improving the user experience of gynecological patients.

Description

Technical Field

[0001] The present invention relates to the field of gynecological lotions, and in particular to a rapid bacteriostatic and low-irritation gynecological lotion and a preparation method thereof. Background Art

[0002] With economic development, improved living standards, and heightened awareness of health, women are increasingly concerned about their own health. Gynecological diseases are the natural enemy of women's health, with 90% of adult women experiencing varying degrees of gynecological diseases. Vaginitis is a common and frequently occurring gynecological disease. According to statistics from the Ministry of Health, the incidence of symptomatic vaginitis in women throughout their lives is 75%, with approximately half of these women experiencing multiple recurrences, primarily manifesting as vulvar and vaginal itching, redness and swelling, and increased vaginal discharge. After contracting the disease, the vulva becomes unbearably itchy, and vaginal discharge increases with an odor. These recurring episodes affect women's work and sleep, causing significant inconvenience in their lives, and can be contagious, leading to serious consequences for their physical and mental health.

[0003] Gynecological washes are a convenient treatment for common gynecological ailments. Women with vaginitis often go to pharmacies to purchase these washes for daily genital cleansing. Currently, pharmaceutical-grade gynecological washes on the market include Jie'eryin and Fufang Huangsong Wash, while other brands include Fuyanjie, Kangnaixin, and Kangfuning. Existing gynecological washes either have limited antibacterial effects, failing to achieve rapid antibacterial and odor-eliminating effects, or they offer rapid antibacterial effects but are excessively irritating to the vaginal skin, resulting in a poor user experience. Many women regularly use these washes to cleanse their vagina. The female vagina is an acidic environment with self-purifying and antibacterial properties. Long-term use of these washes can kill beneficial vaginal bacteria, weakening local resistance and leading to infection. The overuse or misuse of antibiotics can lead to drug resistance in bacteria, kill a significant number of beneficial bacteria, and disrupt the balance of vaginal flora, leading to the growth of fungi and making gynecological diseases difficult to cure.

[0004] Therefore, it is necessary to develop a gynecological wash that is fast in inhibiting bacteria and has low irritation to relieve the discomfort caused by inflammation and improve user experience. Summary of the Invention

[0005] In view of this, the present invention provides a rapid antibacterial and low-irritation gynecological lotion and a preparation method thereof.

[0006] The technical solution of the present invention is achieved as follows:

[0007] A rapid antibacterial, low-irritation gynecological wash comprises the following ingredients, in parts by weight: 6-10 parts of water-soluble composite microparticles, 0.5-0.8 parts of a pH regulator, 1-3 parts of a probiotic lysate, 0.6-1.0 parts of water-soluble vitamin E, 12-15 parts of a natural plant extract, and 0.3-0.5 parts of a preservative.

[0008] The water-soluble composite particles are nano-scale water-soluble composite particles prepared by embedding a water-soluble material and a detergent in a mass ratio of 1:5-7;

[0009] The natural plant extracts include, by weight, 4-6 parts of toona sinensis bud extract, 4-6 parts of snow lotus extract, 2-4 parts of stemona radix extract, 1-3 parts of sophora flavescens extract, 1-3 parts of phellodendron amurense extract, 1-3 parts of cnidium monnieri extract, 2-4 parts of hamamelis virginiana extract, and 2-4 parts of motherwort extract;

[0010] The probiotic lysate is prepared by lysing Lactococcus acidophilus, Bifidobacterium and Bifidobacterium longifolium in a mass ratio of 1.6-1.8:1:0.5-0.8.

[0011] Furthermore, the water-soluble material is any one of hydroxyethyl cellulose, polyvinyl alcohol and epoxidized starch.

[0012] Furthermore, the cleaning agent includes 3-5 parts of dipropylene glycol, 1-3 parts of dichlorobenzyl alcohol and 4-6 parts of o-cymene-5-ol.

[0013] Furthermore, the acid-base regulator is lactic acid and citric acid in a mass ratio of 3-5:1.

[0014] Furthermore, the preservative is phenoxyethanol or paraben.

[0015] Furthermore, the preparation steps of the water-soluble composite particles include: mixing a detergent and a water-soluble material and ultrasonically dispersing the mixture for 30-40 minutes, evaporating the solvent, and solidifying the mixture to obtain nano-scale water-soluble composite particles.

[0016] Furthermore, the preparation steps of the probiotic lysate include: inoculating probiotics in MRS culture medium to prepare a viable bacteria concentration of 1.08×10 10 -1.35×10 10 CFU / L, culture at 36.5℃-37.5℃ for 20-24 hours, collect the bacteria by centrifugation, and lyse with ultrasound for 15-20 minutes to obtain a lysis product, which is then subjected to ultrafiltration and dialysis, and the ultrafiltrate is freeze-dried to obtain a probiotic lysate.

[0017] Furthermore, the centrifugal speed is 2000-2500 r / min, and the time is 10-15 minutes.

[0018] Furthermore, the ultrafiltration dialysis is performed by first performing ultrafiltration dialysis using a 1 kDa ultrafiltration membrane to obtain a retentate, and then performing ultrafiltration dialysis on the retentate using a 3 kDa ultrafiltration membrane.

[0019] A method for preparing a fast-antibacterial, low-irritation gynecological wash comprises the following steps: uniformly stirring water-soluble composite particles, probiotic lysate, and natural plant extracts; then adding water-soluble vitamin E and continuing to uniformly stir; and finally adding an acid-base regulator and a preservative to adjust the pH to 4.5-5.5. Discharging the mixture yields the target gynecological wash.

[0020] Compared with the prior art, the present invention has the following beneficial effects:

[0021] 1. The gynecological lotion of the present invention can not only achieve the effect of rapid antibacterial effect and eliminate odor, but also reduce irritation to the vaginal skin.

[0022] 2. The natural plant extracts of the present invention are selected from toon bud extract, snow lotus extract, stemona extract, sophora flavescens extract, phellodendron amurense extract, cnidium monnieri extract, hamamelis virginiana extract and motherwort extract. The components of each extract are matched without destroying the effective ingredients, which not only enhances the antibacterial effect, eliminates odor, adds soothing and moisturizing ingredients, but also reduces irritation to the vaginal skin.

[0023] 3. The water-soluble composite microparticles of the present invention are quickly released and exert their effects when in contact with water. The nano-sized particles increase the contact area with water, enhance permeability, and promote antibacterial effects. The water-soluble composite microparticles embed the detergent in the water-soluble material, thereby improving the stability of the gynecological wash.

[0024] 4. The present invention uses a probiotic lysate obtained by lysing Lactococcus acidophilus, Bifidobacterium and Bifidobacterium longifolium in a specific ratio, which can not only maintain the normal balance of vaginal flora, regulate vaginal acid-base balance, reduce infection, reduce odor and itching, but also enhance the immunity of vaginal mucosa and reduce the risk of infection. The active ingredients in the probiotic lysate can promote the healing and repair of vaginal mucosa and relieve inflammation and discomfort symptoms. DETAILED DESCRIPTION

[0025] In order to better understand the technical content of the present invention, specific examples are provided below to further illustrate the present invention.

[0026] Unless otherwise specified, the experimental methods used in the examples of the present invention are all conventional methods.

[0027] Unless otherwise specified, the materials, reagents, etc. used in the examples of the present invention can be obtained from commercial sources.

[0028] Lactococcus acidophilus was purchased from Shandong Phoenix Biotechnology Co., Ltd., and Bifidobacterium and Micrococcus longifolia were purchased from Shandong Junkang Biotechnology Research Institute Co., Ltd.

[0029] Example 1

[0030] A fast-bacteriostatic, low-irritation gynecological wash comprises the following raw materials, in parts by weight: 8 parts of water-soluble composite microparticles, 0.7 parts of an acid-base regulator, 2 parts of a probiotic lysate, 0.8 parts of water-soluble vitamin E, 13 parts of a natural plant extract, and 0.4 parts of phenoxyethanol, wherein the acid-base regulator is lactic acid and citric acid in a mass ratio of 4:1, the probiotic lysate is prepared by lysing Lactococcus acidophilus, Bifidobacterium and Bifidobacterium longifolium in a mass ratio of 1.7:1:0.7, and the natural plant extract comprises, in parts by weight, 5 parts of a toon bud extract, 5 parts of a snow lotus extract, 3 parts of a stemona root extract, 2 parts of a sophora flavescens extract, 2 parts of a cork bark extract, 2 parts of a cnidium monnieri extract, 3 parts of a hamamelis virginiana extract, and 3 parts of a motherwort extract.

[0031] The preparation steps of the above-mentioned rapid antibacterial low-irritation gynecological lotion include:

[0032] S1. Preparation of water-soluble composite microparticles: 4 parts of dipropylene glycol, 2 parts of dichlorobenzyl alcohol, 5 parts of o-cymene-5-ol, and hydroxyethyl cellulose were mixed in a mass ratio of 6:1 between the detergent composed of dipropylene glycol, dichlorobenzyl alcohol, and o-cymene-5-ol and the hydroxyethyl cellulose. The mixture was ultrasonically dispersed for 35 minutes, the solvent was evaporated, and the mixture was solidified to obtain nanoscale water-soluble composite microparticles.

[0033] S2. Preparation of probiotic lysate: Lactococcus acidophilus, Bifidobacterium and Bifidobacterium longifolium were inoculated into MRS medium (MRS medium selective for Lactococcus acidophilus) to prepare a viable bacterial concentration of 1.17×10 10 CFU / L, cultured at 37°C for 22h, the fermentation broth was centrifuged at 2300r / min for 13min, the bacteria were collected, and ultrasonic lysis was performed for 18min. The lysis product was first ultrafiltered and dialyzed with a 1kDa ultrafiltration membrane to obtain a retentate, and the retentate was further ultrafiltered and dialyzed with a 3kDa ultrafiltration membrane. The ultrafiltrate was freeze-dried to prepare a probiotic lysate.

[0034] S3. Preparation of gynecological lotion: Mix water-soluble composite particles, probiotic lysate, toon bud extract, snow lotus extract, Stemona extract, Sophora flavescens extract, Phellodendron amurense extract, Cnidium monnieri extract, Hamamelis virginiana extract and Leonurus japonicus extract, then add water-soluble vitamin E and continue to stir evenly. Finally, add lactic acid, citric acid and phenoxyethanol, adjust the pH to 5±0.1, and discharge the material to obtain the target gynecological lotion.

[0035] Example 2

[0036] A fast-bacteriostatic, low-irritation gynecological wash comprises the following raw materials, in parts by weight: 6 parts of water-soluble composite microparticles, 0.5 parts of an acid-base regulator, 1 part of a probiotic lysate, 0.6 parts of water-soluble vitamin E, 12 parts of natural plant extracts, and 0.3 parts of phenoxyethanol, wherein the acid-base regulator is lactic acid and citric acid in a mass ratio of 3:1, the probiotic lysate is prepared by lysing Lactococcus acidophilus, Bifidobacterium and Bifidobacterium longifolium in a mass ratio of 1.6:1:0.5, and the natural plant extracts comprise, in parts by weight, 4 parts of toona sinensis bud extract, 4 parts of snow lotus extract, 2 parts of stemona radix extracts, 1 part of sophora flavescens extract, 1 part of phellodendron amurense extract, 1 part of cnidium monnieri extract, 2 parts of hamamelis virginiana extract, and 2 parts of motherwort extract.

[0037] The preparation steps of the above-mentioned rapid antibacterial low-irritation gynecological lotion include:

[0038] S1. Preparation of water-soluble composite microparticles: 4 parts of dipropylene glycol, 2 parts of dichlorobenzyl alcohol, 5 parts of o-cymene-5-ol, and hydroxyethyl cellulose were mixed in a mass ratio of 6:1 between the detergent composed of dipropylene glycol, dichlorobenzyl alcohol, and o-cymene-5-ol and the hydroxyethyl cellulose. The mixture was ultrasonically dispersed for 35 minutes, the solvent was evaporated, and the mixture was solidified to obtain nanoscale water-soluble composite microparticles.

[0039] S2. Preparation of probiotic lysate: Inoculate Lactococcus acidophilus, Bifidobacterium and Bifidobacterium longifolium into MRS medium (selective medium for Lactococcus acidophilus) to prepare a viable bacterial concentration of 1.17×10 10 CFU / L, cultured at 37°C for 22h, the fermentation broth was centrifuged at 2300r / min for 13min, the bacteria were collected, and ultrasonic lysis was performed for 18min. The lysis product was first ultrafiltered and dialyzed with a 1kDa ultrafiltration membrane to obtain a retentate, and the retentate was further ultrafiltered and dialyzed with a 3kDa ultrafiltration membrane. The ultrafiltrate was freeze-dried to prepare a probiotic lysate.

[0040] S3. Preparation of gynecological lotion: Mix water-soluble composite particles, probiotic lysate, toon bud extract, snow lotus extract, Stemona extract, Sophora flavescens extract, Phellodendron amurense extract, Cnidium monnieri extract, Hamamelis virginiana extract and Leonurus japonicus extract, then add water-soluble vitamin E and continue to stir evenly. Finally, add lactic acid, citric acid and phenoxyethanol, adjust the pH to 5±0.1, and discharge the material to obtain the target gynecological lotion.

[0041] Example 3

[0042] A fast-bacteriostatic, low-irritation gynecological wash comprises the following raw materials, in parts by weight: 10 parts of water-soluble composite microparticles, 0.8 parts of an acid-base regulator, 3 parts of a probiotic lysate, 1 part of water-soluble vitamin E, 15 parts of a natural plant extract, and 0.5 parts of phenoxyethanol, wherein the acid-base regulator is lactic acid and citric acid in a mass ratio of 5:1, the probiotic lysate is prepared by lysing Lactococcus acidophilus, Bifidobacterium and Bifidobacterium longifolium in a mass ratio of 1.8:1:0.8, and the natural plant extract comprises, in parts by weight, 6 parts of a toon bud extract, 6 parts of a snow lotus extract, 4 parts of a stemona root extract, 3 parts of a sophora flavescens extract, 3 parts of a cork bark extract, 3 parts of a cnidium monnieri extract, 4 parts of a witch hazel extract, and 4 parts of a motherwort extract.

[0043] The preparation steps of the above-mentioned rapid antibacterial low-irritation gynecological lotion include:

[0044] S1. Preparation of water-soluble composite microparticles: 4 parts of dipropylene glycol, 2 parts of dichlorobenzyl alcohol, 5 parts of o-cymene-5-ol, and hydroxyethyl cellulose were mixed in a mass ratio of 6:1 between the detergent composed of dipropylene glycol, dichlorobenzyl alcohol, and o-cymene-5-ol and the hydroxyethyl cellulose. The mixture was ultrasonically dispersed for 35 minutes, the solvent was evaporated, and the mixture was solidified to obtain nanoscale water-soluble composite microparticles.

[0045] S2. Preparation of probiotic lysate: Inoculate Lactococcus acidophilus, Bifidobacterium and Bifidobacterium longifolium into MRS medium (selective medium for Lactococcus acidophilus) to prepare a viable bacterial concentration of 1.17×10 10 CFU / L, cultured at 37°C for 22h, the fermentation broth was centrifuged at 2300r / min for 13min, the bacteria were collected, and ultrasonic lysis was performed for 18min. The lysis product was first ultrafiltered and dialyzed with a 1kDa ultrafiltration membrane to obtain a retentate, and the retentate was further ultrafiltered and dialyzed with a 3kDa ultrafiltration membrane. The ultrafiltrate was freeze-dried to prepare a probiotic lysate.

[0046] S3. Preparation of gynecological lotion: Mix water-soluble composite particles, probiotic lysate, toon bud extract, snow lotus extract, Stemona extract, Sophora flavescens extract, Phellodendron amurense extract, Cnidium monnieri extract, Hamamelis virginiana extract and Leonurus japonicus extract, then add water-soluble vitamin E and continue to stir evenly. Finally, add lactic acid, citric acid and phenoxyethanol, adjust the pH to 5±0.1, and discharge the material to obtain the target gynecological lotion.

[0047] Example 4

[0048] A fast-bacteriostatic, low-irritation gynecological wash comprises the following raw materials, in parts by weight: 8 parts of water-soluble composite microparticles, 0.7 parts of an acid-base regulator, 2 parts of a probiotic lysate, 0.8 parts of water-soluble vitamin E, 13 parts of a natural plant extract, and 0.4 parts of phenoxyethanol, wherein the acid-base regulator is lactic acid and citric acid in a mass ratio of 4:1, the probiotic lysate is prepared by lysing Lactococcus acidophilus, Bifidobacterium and Bifidobacterium longifolium in a mass ratio of 1.7:1:0.7, and the natural plant extract comprises, in parts by weight, 5 parts of a toon bud extract, 5 parts of a snow lotus extract, 3 parts of a stemona root extract, 2 parts of a sophora flavescens extract, 2 parts of a cork bark extract, 2 parts of a cnidium monnieri extract, 3 parts of a hamamelis virginiana extract, and 3 parts of a motherwort extract.

[0049] The preparation steps of the above-mentioned rapid antibacterial low-irritation gynecological lotion include:

[0050] S1. Preparation of water-soluble composite microparticles: 3 parts of dipropylene glycol, 1 part of dichlorobenzyl alcohol, 4 parts of o-cymene-5-ol, and hydroxyethyl cellulose were mixed in a mass ratio of 5:1 between the detergent composed of dipropylene glycol, dichlorobenzyl alcohol, and o-cymene-5-ol and the hydroxyethyl cellulose. The mixture was ultrasonically dispersed for 30 minutes, the solvent was evaporated, and the mixture was solidified to obtain nanoscale water-soluble composite microparticles.

[0051] S2. Preparation of probiotic lysate: Inoculate Lactococcus acidophilus, Bifidobacterium and Bifidobacterium longifolium into MRS medium (selective medium for Lactococcus acidophilus) to prepare a viable bacterial concentration of 1.08×10 10 CFU / L, cultured at 36.5℃ for 20h, the fermentation broth was centrifuged at 2000r / min for 10min, the bacteria were collected, and ultrasonic lysis was performed for 15min. The lysis product was first ultrafiltered and dialyzed with a 1kDa ultrafiltration membrane to obtain a retentate, and the retentate was further ultrafiltered and dialyzed with a 3kDa ultrafiltration membrane. The ultrafiltrate was freeze-dried to prepare a probiotic lysate.

[0052] S3. Preparation of gynecological lotion: Mix water-soluble composite particles, probiotic lysate, toon bud extract, snow lotus extract, Stemona extract, Sophora flavescens extract, Phellodendron amurense extract, Cnidium monnieri extract, Hamamelis virginiana extract and Leonurus japonicus extract, then add water-soluble vitamin E and continue to stir evenly. Finally, add lactic acid, citric acid and phenoxyethanol, adjust the pH to 5±0.1, and discharge the material to obtain the target gynecological lotion.

[0053] Example 5

[0054] A fast-bacteriostatic, low-irritation gynecological wash comprises the following raw materials, in parts by weight: 8 parts of water-soluble composite microparticles, 0.7 parts of an acid-base regulator, 2 parts of a probiotic lysate, 0.8 parts of water-soluble vitamin E, 13 parts of a natural plant extract, and 0.4 parts of phenoxyethanol, wherein the acid-base regulator is lactic acid and citric acid in a mass ratio of 4:1, the probiotic lysate is prepared by lysing Lactococcus acidophilus, Bifidobacterium and Bifidobacterium longifolium in a mass ratio of 1.7:1:0.7, and the natural plant extract comprises, in parts by weight, 5 parts of a toon bud extract, 5 parts of a snow lotus extract, 3 parts of a stemona root extract, 2 parts of a sophora flavescens extract, 2 parts of a cork bark extract, 2 parts of a cnidium monnieri extract, 3 parts of a hamamelis virginiana extract, and 3 parts of a motherwort extract.

[0055] The preparation steps of the above-mentioned rapid antibacterial low-irritation gynecological lotion include:

[0056] S1. Preparation of water-soluble composite microparticles: 5 parts of dipropylene glycol, 3 parts of dichlorobenzyl alcohol, 6 parts of o-cymene-5-ol, and hydroxyethyl cellulose were mixed in a mass ratio of 7:1 between the detergent composed of dipropylene glycol, dichlorobenzyl alcohol, and o-cymene-5-ol and the hydroxyethyl cellulose. The mixture was ultrasonically dispersed for 35 minutes, the solvent was evaporated, and the mixture was solidified to obtain nanoscale water-soluble composite microparticles.

[0057] S2. Preparation of probiotic lysate: Inoculate Lactococcus acidophilus, Bifidobacterium and Bifidobacterium longifolium into MRS medium (selective medium for Lactococcus acidophilus) to prepare a viable bacterial concentration of 1.35×10 10 CFU / L, cultured at 37.5℃ for 24h, the fermentation broth was centrifuged at 2500r / min for 15min, the bacteria were collected, and ultrasonic lysis was performed for 20min. The lysis product was first ultrafiltered and dialyzed with a 1kDa ultrafiltration membrane to obtain a retentate, and the retentate was further ultrafiltered and dialyzed with a 3kDa ultrafiltration membrane. The ultrafiltrate was freeze-dried to prepare a probiotic lysate.

[0058] S3. Preparation of gynecological lotion: Mix water-soluble composite particles, probiotic lysate, toon bud extract, snow lotus extract, Stemona extract, Sophora flavescens extract, Phellodendron amurense extract, Cnidium monnieri extract, Hamamelis virginiana extract and Leonurus japonicus extract, then add water-soluble vitamin E and continue to stir evenly. Finally, add lactic acid, citric acid and phenoxyethanol, adjust the pH to 5±0.1, and discharge the material to obtain the target gynecological lotion.

[0059] Comparative Example 1

[0060] The difference from Example 1 is that the amount of raw materials is different; the rest is the same as Example 1.

[0061] A fast-bacteriostatic, low-irritation gynecological wash comprises the following raw materials, in parts by weight: 5 parts of water-soluble composite microparticles, 0.9 parts of an acid-base regulator, 4 parts of a probiotic lysate, 0.4 parts of water-soluble vitamin E, 10 parts of a natural plant extract, and 0.1 parts of phenoxyethanol, wherein the acid-base regulator is lactic acid and citric acid in a mass ratio of 2:1, the probiotic lysate is prepared by lysing Lactococcus acidophilus, Bifidobacterium and Bifidobacterium longifolium in a mass ratio of 1:1:1, and the natural plant extract comprises, in parts by weight, 3 parts of a toon bud extract, 3 parts of a snow lotus extract, 1 part of a stemona root extract, 0.5 parts of a sophora flavescens extract, 0.5 parts of a cork bark extract, 0.5 parts of a cnidium monnieri extract, 1 part of a hamamelis virginiana extract, and 1 part of a motherwort extract.

[0062] Comparative Example 2

[0063] The difference from Example 1 is that: the natural plant extracts are missing, that is, the toon bud extract, snow lotus extract, stemona extract, sophora flavescens extract, phellodendron amurense extract, cnidium monnieri extract, hamamelis virginiana extract and motherwort extract are missing; the rest is consistent with Example 1.

[0064] A fast-bacteriostatic, low-irritation gynecological wash comprises the following raw materials, in parts by weight: 8 parts of water-soluble composite particles, 0.7 parts of an acid-base regulator, 2 parts of a probiotic lysate, 0.8 parts of water-soluble vitamin E, and 0.4 parts of phenoxyethanol. The acid-base regulator is lactic acid and citric acid in a mass ratio of 4:1, and the probiotic lysate is prepared by lysing Lactococcus acidophilus, Bifidobacterium, and Bifidobacterium longifolium in a mass ratio of 1.7:1:0.7.

[0065] The preparation steps of the above-mentioned rapid antibacterial low-irritation gynecological lotion include:

[0066] S1. Preparation of water-soluble composite microparticles: 4 parts of dipropylene glycol, 2 parts of dichlorobenzyl alcohol, 5 parts of o-cymene-5-ol, and hydroxyethyl cellulose were mixed in a mass ratio of 6:1 between the detergent composed of dipropylene glycol, dichlorobenzyl alcohol, and o-cymene-5-ol and the hydroxyethyl cellulose. The mixture was ultrasonically dispersed for 35 minutes, the solvent was evaporated, and the mixture was solidified to obtain nanoscale water-soluble composite microparticles.

[0067] S2. Preparation of probiotic lysate: Inoculate Lactococcus acidophilus, Bifidobacterium and Bifidobacterium longifolium into MRS medium (selective medium for Lactococcus acidophilus) to prepare a viable bacterial concentration of 1.17×10 10 CFU / L, cultured at 37°C for 22h, the fermentation broth was centrifuged at 2300r / min for 13min, the bacteria were collected, and ultrasonic lysis was performed for 18min. The lysis product was first ultrafiltered and dialyzed with a 1kDa ultrafiltration membrane to obtain a retentate, and the retentate was further ultrafiltered and dialyzed with a 3kDa ultrafiltration membrane. The ultrafiltrate was freeze-dried to prepare a probiotic lysate.

[0068] S3. Preparation of gynecological lotion: Mix the water-soluble composite microparticles and the probiotic lysate and stir evenly, then add water-soluble vitamin E and continue stirring evenly, finally add lactic acid, citric acid and phenoxyethanol, adjust the pH to 5±0.1, and discharge the material to obtain the target gynecological lotion.

[0069] Comparative Example 3

[0070] The difference from Example 1 is that the water-soluble material in the water-soluble composite particles is missing, that is, there is only a cleaning agent composed of dipropylene glycol, dichlorobenzyl alcohol and o-cymene-5-ol; the rest is the same as Example 1.

[0071] A fast-bacteriostatic, low-irritation gynecological wash comprises the following raw materials, in parts by weight: 4 parts of dipropylene glycol, 2 parts of dichlorobenzyl alcohol, 5 parts of o-cymene-5-ol, 0.7 parts of an acid-base regulator, 2 parts of a probiotic lysate, 0.8 parts of water-soluble vitamin E, 13 parts of a natural plant extract, and 0.4 parts of phenoxyethanol, wherein the acid-base regulator comprises lactic acid and citric acid in a mass ratio of 4:1, the probiotic lysate is prepared by lysing Lactococcus acidophilus, Bifidobacterium and Bifidobacterium longifolium in a mass ratio of 1.7:1:0.7, and the natural plant extract comprises, in parts by weight, 5 parts of a toon bud extract, 5 parts of a snow lotus extract, 3 parts of a stemona root extract, 2 parts of a sophora flavescens extract, 2 parts of a cork bark extract, 2 parts of a cnidium monnieri extract, 3 parts of a hamamelis virginiana extract, and 3 parts of a motherwort extract.

[0072] The preparation steps of the above-mentioned rapid antibacterial low-irritation gynecological lotion include:

[0073] S1. Preparation of probiotic lysate: Inoculate Lactococcus acidophilus, Bifidobacterium and Bifidobacterium longifolium into MRS medium (selective medium for Lactococcus acidophilus) to prepare a viable bacterial concentration of 1.17×10 10 CFU / L, cultured at 37°C for 22h, the fermentation broth was centrifuged at 2300r / min for 13min, the bacteria were collected, and ultrasonic lysis was performed for 18min. The lysis product was first ultrafiltered and dialyzed with a 1kDa ultrafiltration membrane to obtain a retentate, and the retentate was further ultrafiltered and dialyzed with a 3kDa ultrafiltration membrane. The ultrafiltrate was freeze-dried to prepare a probiotic lysate.

[0074] S2. Preparation of gynecological lotion: Dipropylene glycol, dichlorobenzyl alcohol, o-cymene-5-ol, probiotic lysate, toona sinensis bud extract, snow lotus extract, Stemona root extract, Sophora flavescens extract, Phellodendron amurense extract, Cnidium monnieri extract, Hamamelis virginiana extract and Leonurus japonicus extract are mixed evenly, then water-soluble vitamin E is added and the mixture is stirred evenly, and finally lactic acid, citric acid and phenoxyethanol are added, and the pH is adjusted to 5±0.1. The target gynecological lotion is obtained by discharging the material.

[0075] Comparative Example 4

[0076] The difference from Example 1 is that the probiotic lysate is prepared by lysing Lactococcus acidophilus, Bifidobacterium bifidum and Bifidobacterium longifolium in a mass ratio of 1:2:1; the rest is the same as Example 1.

[0077] A fast-bacteriostatic, low-irritation gynecological wash comprises the following raw materials, in parts by weight: 8 parts of water-soluble composite microparticles, 0.7 parts of an acid-base regulator, 2 parts of a probiotic lysate, 0.8 parts of water-soluble vitamin E, 13 parts of a natural plant extract, and 0.4 parts of phenoxyethanol, wherein the acid-base regulator is lactic acid and citric acid in a mass ratio of 4:1, the probiotic lysate is prepared by lysing Lactococcus acidophilus, Bifidobacterium and Bifidobacterium longifolium in a mass ratio of 1:2:1, and the natural plant extract comprises, in parts by weight, 5 parts of a toon bud extract, 5 parts of a snow lotus extract, 3 parts of a stemona root extract, 2 parts of a sophora flavescens extract, 2 parts of a cork bark extract, 2 parts of a cnidium monnieri extract, 3 parts of a witch hazel extract, and 3 parts of a motherwort extract.

[0078] Antibacterial performance test

[0079] Bacteria used in the in vitro antibacterial test: Candida albicans (ATCC 10231), Escherichia coli (ATCC 25922), Staphylococcus aureus (ATCC 6538).

[0080] According to the method specified in the "Technical Specifications for Disinfection" (2002 edition), the gynecological lotions prepared in Examples 1-5 of the present invention and Comparative Examples 1-4 were subjected to action for 2 min, 5 min, 10 min, and 20 min, respectively. The experiment was repeated 3 times, and the average sterilization rate was taken. The test results are shown in Table 1.

[0081] Table 1

[0082]

[0083]

[0084] As can be seen from Table 1, the gynecological lotions prepared in Examples 1-5 of the present invention have a rapid bactericidal effect on Staphylococcus aureus, Escherichia coli and Candida albicans, can efficiently and quickly kill bacteria in a short time, and can inhibit bacteria for a long time. Comparative Example 1 differs from Example 1 in that the raw material dosage is different, resulting in a decrease in the antibacterial effect of the gynecological lotion, and it is impossible to reach a 100% antibacterial rate in a short time. Comparative Example 2 differs from Example 1 in that it lacks natural plant extracts (Toona sinensis bud extract, Snow Lotus extract, Stemona root extract, Sophora flavescens extract, Phellodendron chinense extract, Cnidium monnieri extract, Hamamelis virginiana extract and Leonurus herba extract), resulting in a significant decrease in the antibacterial rate of the gynecological lotion, indicating that the composition of the extracts in the gynecological lotion prepared by the present invention neither destroys the active ingredients nor enhances the antibacterial effect and eliminates odor. A large number of experiments have shown that toona sinensis bud extract, snow lotus extract, stemona root extract, sophora flavescens extract, phellodendron chinense extract, cnidium monnieri extract, hamamelis virginiana extract and motherwort extract synergistically enhance the antibacterial effect. The antibacterial effect of gynecological washes prepared in different proportions or different combinations (lacking a certain extract or replacing a certain extract) has decreased to varying degrees, which will not be described in detail here. Comparative Example 3 differs from Example 1 in that the water-soluble material in the water-soluble composite particles is missing, and the detergent composed of only dipropylene glycol, dichlorobenzyl alcohol and o-cymene-5-ol decreases its antibacterial effect. This is because the nanoscale water-soluble composite particles increase the contact area with water, enhance permeability, and promote the antibacterial effect. Comparative example 4 and embodiment 1 difference is that probiotic lysate is by the lactococcus acidophilus of mass ratio 1:2:1, bifidobacterium and bifidobacterium longifolia micrococcus cracking and obtain, its antibacterial effect also declines, show the probiotic lysate that the lactococcus acidophilus of specific ratio of the present invention, bifidobacterium and bifidobacterium longifolia micrococcus cracking obtain, not only can maintain vaginal normal flora balance, regulate vaginal acid-base balance, reduce infection, reduce peculiar smell and pruritus, can enhance the immunity of vaginal mucosa simultaneously, reduce the risk of infection, the active component in probiotic lysate can promote the healing and the repair of vaginal mucosa, can alleviate inflammation and malaise symptoms.A large number of experiments show, if the gynecological lotion made of the ratio of probiotic bacterium of the present invention and composition (lacking a certain probiotic bacterium or replacing a certain probiotic bacterium), its antibacterial effect all has decline in various degrees, do not repeat them one by one at this.

[0085] Vaginal mucosal irritation test

[0086] Experimental rabbits were randomly divided into a test group (Examples 1-5), a control group (Comparative Examples 1-4), and a blank group, with 5 rabbits in each group. Before the experiment, the vaginal opening of each rabbit was inspected for discharge, congestion, and other injuries. A blunt-tipped flexible tube was connected to a 5 mL syringe. The rabbit was positioned supine, exposing the perineum and vaginal opening. A catheter moistened with the test solution was gently inserted 4-5 cm into the vagina. 2 mL of the test solution (gynecological lotion prepared in Examples 1-5 and Comparative Examples 1-4) was slowly injected using a syringe. The flexible tube was gently withdrawn. The blank group was treated with normal saline in the same manner. This procedure was repeated every 24 hours for 5 consecutive days. The animals were sacrificed using the air embolism method, and the intact vagina was removed. The rabbit was observed for congestion, edema, and other signs, and histopathological sections were examined. The experimental results are shown in Table 2.

[0087] Table 2

[0088]

[0089] As seen from Table 2, the gynecological lotion of embodiment 1-5 is negative to vaginal mucosa irritation test result, shows that gynecological lotion product of the present invention is non-irritating, all with good biosafety.Comparative example 1 is different from Example 1 in that raw material dosage is different, and unscientific composition is composited, causes irritation to vaginal mucosa, comparative example 2 is different from Example 1 in that lacks natural plant extract (Toona sinensis bud extract, Snow Lotus flower extract, Radix Stemonae extract, Sophorae Flavescentis extract, Cortex Phellodendri extract, Fructus Cnidium monnieri extract, Hamamelis Virginiana extract and Herba Leonuri extract), lacks soothing and moisturizing ingredients Toona sinensis bud extract, Herba Leonuri extract and Snow Lotus flower extract, causes gynecological lotion to have irritation.Comparative example 4 is different from Example 1 in that probiotic lysate is made by the cracking of lactococcus acidophilus, bifidobacterium and bifidobacterium longifolium micrococcus of mass ratio 1:2:1, it has irritation, shows that the probiotic lysate made by the cracking of lactococcus acidophilus, bifidobacterium and bifidobacterium longifolium micrococcus of specific ratio of the present invention, can not only maintain vaginal normal flora balance, reduce irritation. A large number of experiments have shown that if the gynecological lotion made with the proportion and composition of the probiotics of the present invention (lacking a certain probiotic or replacing a certain probiotic) will have different degrees of irritation, which will not be described in detail here.

[0090] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A rapid antibacterial, low-irritation gynecological lotion, characterized in that: The composition is composed of the following raw materials in parts by weight: 6-10 parts of water-soluble composite microparticles, 0.5-0.8 parts of acid-base regulator, 1-3 parts of probiotic lysate, 0.6-1.0 parts of water-soluble vitamin E, 12-15 parts of natural plant extracts, and 0.3-0.5 parts of preservatives; The water-soluble composite particles are nano-scale water-soluble composite particles prepared by embedding a water-soluble material and a detergent in a mass ratio of 1:5-7; the water-soluble material is hydroxyethyl cellulose; The natural plant extract is composed of the following raw materials in parts by weight: 4-6 parts of toona sinensis bud extract, 4-6 parts of snow lotus extract, 2-4 parts of stemona radix extract, 1-3 parts of sophora flavescens extract, 1-3 parts of phellodendron amurense extract, 1-3 parts of cnidium monnieri extract, 2-4 parts of hamamelis virginiana extract and 2-4 parts of motherwort extract; The probiotic lysate is prepared by lysing Lactococcus acidophilus, Bifidobacterium and Bifidobacterium longifolium in a mass ratio of 1.6-1.8:1:0.5-0.

8.

2. A rapid antibacterial, low-irritation gynecological lotion according to claim 1, characterized in that: The cleaning agent comprises 3-5 parts of dipropylene glycol, 1-3 parts of dichlorobenzyl alcohol and 4-6 parts of o-cymene-5-ol.

3. A rapid antibacterial, low-irritation gynecological lotion according to claim 1, characterized in that: The acid-base regulator is lactic acid and citric acid in a mass ratio of 3-5:

1.

4. A rapid antibacterial, low-irritation gynecological lotion according to claim 1, characterized in that: The preservative is phenoxyethanol or paraben.

5. The rapid antibacterial and low-irritation gynecological lotion according to claim 1, characterized in that: The preparation steps of the water-soluble composite particles include: mixing a detergent and a water-soluble material and ultrasonically dispersing the mixture for 30-40 minutes, evaporating and removing the solvent, and solidifying the mixture to obtain nano-scale water-soluble composite particles.

6. A rapid antibacterial, low-irritation gynecological lotion according to claim 1, characterized in that: The preparation steps of the probiotic lysate include: inoculating probiotics in MRS culture medium, preparing a viable bacteria concentration of 1.08×10 10 -1.35×10 10 CFU / L, culture at 36.5℃-37.5℃ for 20-24 hours, collect the bacteria by centrifugation, and lyse with ultrasound for 15-20 minutes to obtain a lysis product, which is then subjected to ultrafiltration and dialysis, and the ultrafiltrate is freeze-dried to obtain a probiotic lysate.

7. A rapid antibacterial, low-irritation gynecological lotion according to claim 6, characterized in that: The centrifugal speed is 2000-2500 r / min, and the time is 10-15 minutes.

8. A rapid antibacterial, low-irritation gynecological lotion according to claim 6, characterized in that: The ultrafiltration and dialysis are performed by firstly performing ultrafiltration and dialysis using a 1 kDa ultrafiltration membrane to obtain a retentate, and then performing ultrafiltration and dialysis on the retentate using a 3 kDa ultrafiltration membrane.

9. The method for preparing a rapid antibacterial and low-irritation gynecological lotion according to any one of claims 1 to 8, characterized in that the steps include: The water-soluble composite particles, probiotic lysate and natural plant extract are stirred evenly, then water-soluble vitamin E is added and stirred evenly, and finally an acid-base regulator and a preservative are added to adjust the pH to 4.5-5.5, and the material is discharged to obtain the target gynecological wash.

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