An anti-aging composition containing Paeonia ostii flower peptides and a preparation method thereof

Abstract

The present invention discloses an anti-aging composition containing Paeonia ostii flower peptides and a preparation method thereof, belonging to the technical field of compositions. By mass, the raw materials of the composition include the following components: 8-10 parts of Paeonia ostii flower peptides, 3-5 parts of compound plant flavonoids, 1-2 parts of chitosan, and 0.3-0.5 parts of sodium ascorbyl phosphate; the compound plant flavonoids are obtained by mixing and extracting Achyrocline satureioides, Taraxacum mongolicum, and Perilla frutescens leaves as raw materials. Different components in the composition with different antioxidant and anti-aging mechanisms and activities synergistically enhance the effects, achieving excellent antioxidant and anti-aging effects.

Description

Technical Field

[0001] The invention relates to the technical field of compositions, and in particular to an anti-aging composition containing Danfeng peony flower peptide and a preparation method thereof. Background Art

[0002] Human aging is a natural law and is closely related to human health. According to the free radical theory, free radicals have a strong oxidative ability, which can damage DNA, thereby affecting human mutations and cell proliferation, leading to a series of degenerative changes in cell morphology and function, including a decrease in protein synthesis rate and an increase in error rate, which in turn causes abnormal gene expression, causing the normal growth regulation and self-homeostasis of human cells to be unbalanced, thereby causing cell aging. As the human body ages, the number of oxygen free radicals in the cells increases, and the ability of cells to remove free radicals gradually decreases, thus causing various damages to accumulate, directly causing human aging. Biologically speaking, aging is a spontaneous and inevitable process of organisms over time, but with the development of science and technology and the progress of human society, people's living standards are constantly improving. For this reason, people are looking forward to extending their lifespan as much as possible without violating the laws of nature, and the demand for anti-aging is becoming more and more urgent. With the growing demand for anti-aging, various anti-aging products have also emerged. The market is full of various medicines and health products with anti-aging effects, but these existing anti-aging products are often mediocre in efficacy, and the development of more products with excellent anti-aging effects is still the focus of the work of those skilled in the art. Summary of the invention

[0003] The purpose of the present invention is to provide an anti-aging composition containing Danfeng peony flower peptide and a preparation method thereof, so as to solve the problems existing in the above-mentioned prior art.

[0004] To achieve the above object, the present invention provides the following solutions:

[0005] One of the technical solutions of the present invention is an anti-aging composition containing Danfeng peony flower peptide, wherein the raw materials include the following components by weight: 8-10 parts of Danfeng peony flower peptide, 3-5 parts of composite plant flavonoids, 1-2 parts of chitosan oligosaccharides and 0.3-0.5 parts of sodium ascorbyl phosphate; the composite plant flavonoids are obtained by mixed extraction of Cynanchum melongena, Taraxacum officinale and Perilla leaf as raw materials.

[0006] Further, the preparation method of the Danfeng peony flower peptide comprises: using Danfeng peony flowers as raw materials, obtaining Danfeng peony flower protein by the alkali extraction and acid precipitation method; formulating the Danfeng peony flower protein into a Danfeng peony flower protein solution, and successively performing ultrasonic pretreatment, composite enzyme hydrolysis, enzyme inactivation, cooling, and centrifugation to obtain a crude Danfeng peony flower peptide solution; subjecting the crude Danfeng peony flower peptide solution to membrane filtration to retain a polypeptide filtrate with a molecular weight cut-off of 1000-10000 Da; and freeze-drying the polypeptide filtrate to obtain the Danfeng peony flower peptide.

[0007] Further, the obtaining of Danfeng peony flower protein by the alkali extraction and acid precipitation method specifically comprises: crushing Danfeng peony flowers to obtain Danfeng peony flower powder; mixing the Danfeng peony flower powder with water, adding an aqueous sodium hydroxide solution to adjust the pH to 8-10, heating and stirring for extraction, cooling after the extraction is completed, centrifuging, taking the supernatant, adjusting the pH to 2-5 with a hydrochloric acid solution, centrifuging again, and drying the precipitate to obtain the Danfeng peony flower protein.

[0008] Further, the mixing ratio of the Danfeng peony flower powder to the water is 1 g:20-30 mL; the temperature for the heating and stirring extraction is 50-60 °C, and the time is 4-6 h; the temperature for the drying is 40-50 °C.

[0009] Further, the power of the ultrasonic pretreatment is 600-700 W, the temperature is 50-60 °C, and the time is 30-60 min.

[0010] The cavitation effect and mechanical effect generated by ultrasound can loosen the originally highly compressed and tight structure of the protein, break the quaternary structure of the protein molecule while opening the protein aggregate, gradually dissociate and stretch the aggregated protein, expose the active sites buried inside the protein, better bind to the enzyme, and improve the hydrolysis degree; in addition, during the stretching process of the protein molecule, internal amino acids such as tyrosine and leucine are fully exposed, and these amino acids play an important role in improving the activity and stability of the polypeptide.

[0011] Further, the composite enzyme comprises acid protease and ficin; the conditions for the composite enzyme hydrolysis are hydrolysis at 50-60 °C for 3-5 h.

[0012] Using acid protease and ficin for composite enzyme hydrolysis of Danfeng peony flower protein can obtain polypeptides with high antioxidant activity.

[0013] Further, the formulation of the Danfeng peony flower protein into a Danfeng peony flower protein solution specifically comprises: mixing the Danfeng peony flower protein and water at a ratio of 5-15 mg:1 mL to obtain a Danfeng peony flower protein solution.

[0014] Further, the specific steps of the hydrolysis by the complex enzyme are as follows: Add a citric acid solution to the protein solution of Paeonia ostii 'Feng Dan' flowers to adjust the pH to 4 - 5, then add the complex enzyme, and heat for hydrolysis.

[0015] Adjusting the pH of the protein solution with citric acid can improve the stability of the antioxidant activity of the subsequently prepared peptides from Paeonia ostii 'Feng Dan' flowers.

[0016] Further, the addition amount of the acid protease is 4 - 6 wt% of the protein of Paeonia ostii 'Feng Dan' flowers, and the addition amount of the ficin is 0.5 - 1 wt% of the protein of Paeonia ostii 'Feng Dan' flowers.

[0017] Further, the temperature for inactivating the enzyme is 80 - 90 °C, and the time is 10 - 30 min.

[0018] Further, before freeze - drying, it also includes the step of adding trehalose to the polypeptide filtrate; the addition amount of trehalose in the polypeptide filtrate is 0.5 - 1.5 mg / mL.

[0019] Adding trehalose to the polypeptide filtrate can improve the stability of the polypeptide. During the freeze - drying process, trehalose can also replace water and form hydrogen bonds with the polypeptide to stabilize the native conformation of the polypeptide, thereby improving the antioxidant activity and stability of the polypeptide. Moreover, trehalose itself has antioxidant activity, and it can also enhance the antioxidant activity of the freeze - dried product.

[0020] Further, the preparation method of the complex plant flavonoids includes: Crushing Galinsoga parviflora, Taraxacum mongolicum, and Perilla frutescens leaves to obtain a complex plant powder; adding an ethanol solution and Tween 80 to the complex plant powder, extracting at a temperature of 60 - 70 °C with the assistance of ultrasonic waves for 1 - 2 h, filtering, and freeze - drying the filtrate to obtain the complex plant flavonoids.

[0021] Further, the mass ratio of Galinsoga parviflora, Taraxacum mongolicum, and Perilla frutescens leaves in the complex plant powder is 6 - 8:3 - 1:1; the dosage ratio of the complex plant powder to the ethanol solution is 1 g:30 - 50 mL; the addition amount of Tween 80 is 2 - 3 mg / mL.

[0022] Galinsoga parviflora, Taraxacum mongolicum, and Perilla frutescens leaves all contain various flavonoid substances with antioxidant activity. Mixing and extracting the three plants can obtain complex flavonoids with diverse components and enhanced antioxidant activity.

[0023] Further, the concentration of the ethanol solution is 40 vol%; the power of the ultrasonic wave is 200 - 300 W.

[0024] The second technical solution of the present invention: A preparation method of the above-mentioned anti-aging composition containing peony flower peptides of Danfeng, taking peony flower peptides of Danfeng, compound plant flavonoids, chitosan oligosaccharide and sodium ascorbyl phosphate according to mass parts, and mixing them evenly to obtain.

[0025] The third technical solution of the present invention: The application of the above-mentioned anti-aging composition containing peony flower peptides of Danfeng in the preparation of anti-aging products.

[0026] The present invention discloses the following technical effects:

[0027] The present invention provides an anti-aging composition containing peony flower peptides of Danfeng. The raw materials include peony flower peptides of Danfeng, compound plant flavonoids, chitosan oligosaccharide and sodium ascorbyl phosphate. Among them, the compound plant flavonoids are obtained by mixing and extracting with Galinsoga parviflora, Taraxacum mongolicum and Perilla frutescens leaves as raw materials. Peony flower peptides of Danfeng have the advantages of safety, stability, easy solubility in water, easy absorption, small molecular weight, etc., and at the same time have various biological activities such as scavenging free radicals, antioxidant and anti-aging; Galinsoga parviflora, Taraxacum mongolicum and Perilla frutescens leaves all contain a variety of flavonoids that can neutralize free radicals, reduce cellular oxidative stress and protect the body from oxidative damage. And the specific types and activity intensities of the flavonoids contained in the three are different. By mixing and extracting with Galinsoga parviflora, Taraxacum mongolicum and Perilla frutescens leaves as raw materials, the flavonoids with different structures and activities in the three can be combined together to obtain compound plant flavonoids with enhanced activity; chitosan oligosaccharide contains rich functional groups such as carboxyl, amino and hydroxyl groups, has strong antioxidant activity, and is easily absorbed and utilized by organisms; sodium ascorbyl phosphate can participate in various reactions in the body, and has functions such as promoting collagen production, blocking melanin synthesis, scavenging free radicals and preventing skin aging; the components in the composition with different categories, different antioxidant and anti-aging mechanisms and activities synergistically enhance the effect, achieving excellent antioxidant and anti-aging effects. Specific Embodiments

[0028] Now, various exemplary embodiments of the present invention will be described in detail. This detailed description should not be considered as a limitation of the present invention, but should be understood as a more detailed description of certain aspects, characteristics and implementation embodiments of the present invention.

[0029] It should be understood that the terms described in the present invention are only for describing specific embodiments and are not used to limit the present invention. In addition, for the numerical ranges in the present invention, it should be understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. Any intermediate value within any stated value or stated range, as well as each smaller range between any other stated value or intermediate value within the stated range, is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded from the range.

[0030] Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. Although this invention only describes preferred methods and materials, any methods and materials similar or equivalent to those described herein can also be used in the practice or testing of this invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials related to the said documents. In case of conflict with any incorporated document, the content of this specification shall prevail.

[0031] Without departing from the scope or spirit of this invention, various improvements and changes can be made to the specific embodiments of the specification of this invention, which are obvious to those skilled in the art. Other embodiments obtained from the specification of this invention are obvious to those skilled in the art. The specification and examples of this invention are merely exemplary.

[0032] Regarding the use of "comprising", "including", "having", "containing", etc. in this article, they are all open-ended terms, meaning including but not limited to.

[0033] The "parts by weight" involved in the following examples and comparative examples all represent "parts by mass".

[0034] All raw materials used in the following examples and comparative examples are ordinary commercially available products; among them, the enzyme activity unit of the acid protease used is 800,000 U / g, and the enzyme activity unit of the ficin used is 800,000 U / g; the raw materials of Paeonia ostii flowers, Galinsoga parviflora, Taraxacum mongolicum, and Perilla frutescens leaves used in the extraction are air-dried in advance.

[0035] The preparation method of Paeonia ostii flower protein used in the following examples and comparative examples is as follows: pulverize Paeonia ostii flowers, pass through an 80-mesh sieve to obtain Paeonia ostii flower powder; mix Paeonia ostii flower powder with water at a ratio of 1 g:25 mL, add 1 mol / L NaOH aqueous solution to adjust the pH to 9, heat and stir at 55 °C for extraction for 5 h; after the extraction is completed, cool to room temperature, centrifuge at 7000 rpm for 12 min, take the supernatant, adjust the pH to 3 with 1 mol / L hydrochloric acid solution, centrifuge again at 7000 rpm for 12 min, and dry the precipitate at 45 °C to obtain Paeonia ostii flower protein.

[0036] Example 1

[0037] An anti-aging composition containing Paeonia ostii flower peptide is obtained by uniformly mixing 8 parts of Paeonia ostii flower peptide, 3 parts of compound plant flavonoids, 1 part of chitosan oligosaccharide, and 0.3 part of sodium ascorbyl phosphate.

[0038] Among them, the preparation method of Paeonia ostii flower peptide is as follows:

[0039] (1) Mix the Paeonia ostii flower protein with water at a ratio of 5 mg:1 mL, and perform ultrasonic pretreatment at a temperature of 50 °C with an ultrasonic power of 600 W for 30 min;

[0040] (2) Add 1 mol / L citric acid solution to the Paeonia ostii flower protein solution after ultrasonic pretreatment to adjust the pH to 4, then add acid protease and ficin (the addition amounts of acid protease and ficin are 5 wt% and 0.5 wt% of the Paeonia ostii flower protein respectively), heat and hydrolyze at a temperature of 50 °C for 3 h. After the enzymatic hydrolysis is completed, raise the temperature to 80 °C to inactivate the enzyme for 10 min. After cooling to room temperature, centrifuge at 7000 rpm for 12 min to obtain the Paeonia ostii flower peptide stock solution. Filter through a membrane to retain the polypeptide filtrate with a molecular weight cut-off of 1000 - 10000 Da. Add trehalose to the polypeptide filtrate (the addition amount of trehalose in the polypeptide filtrate is 0.5 mg / mL), and then perform freeze-drying to obtain Paeonia ostii flower peptide.

[0041] The preparation method of the composite plant flavonoids is as follows: Mix Galinsoga parviflora, Taraxacum mongolicum and Perilla frutescens leaves in a mass ratio of 6:3:1, pulverize them, and pass through an 80-mesh sieve to obtain the composite plant powder; add 40 vol% ethanol solution and Tween 80 to the composite plant powder (the dosage ratio of the composite plant powder to the ethanol solution is 1 g:30 mL; the addition amount of Tween 80 is 2 mg / mL), extract at a temperature of 60 °C with the assistance of ultrasonic waves (ultrasonic power is 200 W) for 1 h, filter, and perform freeze-drying on the filtrate to obtain the composite plant flavonoids.

[0042] Example 2

[0043] An anti-aging composition containing Paeonia ostii flower peptide is obtained by uniformly mixing 9 parts of Paeonia ostii flower peptide, 4 parts of composite plant flavonoids, 1.5 parts of chitosan oligosaccharide and 0.4 part of sodium ascorbyl phosphate.

[0044] Among them, the preparation method of Paeonia ostii flower peptide is as follows:

[0045] (1) Mix the Paeonia ostii flower protein with water at a ratio of 10 mg:1 mL, and perform ultrasonic pretreatment at a temperature of 55 °C with an ultrasonic power of 650 W for 40 min;

[0046] (2) Add 1 mol / L citric acid solution to the protein solution of Paeonia ostii flower after ultrasonic pretreatment to adjust the pH to 4.5, then add acid protease and ficin (the addition amounts of acid protease and ficin are 5 wt% and 0.8 wt% of the Paeonia ostii flower protein respectively), heat and hydrolyze at 55 °C for 4 h. After the enzymatic hydrolysis, raise the temperature to 85 °C to inactivate the enzyme for 20 min. Cool the hydrolyzate after enzyme inactivation to 50 °C, add calcium hydroxide powder to adjust the pH to 7, centrifuge at 7000 rpm for 12 min at room temperature to obtain the crude Paeonia ostii flower peptide solution. Filter through a membrane to retain the polypeptide filtrate with a molecular weight cut-off of 1000 - 10000 Da. Add trehalose to the polypeptide filtrate (the addition amount of trehalose in the polypeptide filtrate is 1 mg / mL), and then freeze-dry to obtain Paeonia ostii flower peptide.

[0047] The preparation method of the composite plant flavonoids is as follows: Mix Galinsoga parviflora, Taraxacum mongolicum and Perilla frutescens leaves in a mass ratio of 7:2:1, pulverize them, and pass through an 80-mesh sieve to obtain the composite plant powder; add 40 vol% ethanol solution and Tween 80 to the composite plant powder (the dosage ratio of the composite plant powder to the ethanol solution is 1 g:40 mL; the addition amount of Tween 80 is 2.5 mg / mL), extract at 65 °C with the assistance of ultrasonic waves (ultrasonic power is 250 W) for 1.5 h, filter, and freeze-dry the filtrate to obtain the composite plant flavonoids.

[0048] Example 3

[0049] An anti-aging composition containing Paeonia ostii flower peptide is obtained by uniformly mixing 10 parts of Paeonia ostii flower peptide, 5 parts of composite plant flavonoids, 2 parts of chitosan oligosaccharide and 0.5 part of sodium ascorbyl phosphate.

[0050] Among them, the preparation method of Paeonia ostii flower peptide is as follows:

[0051] (1) Mix Paeonia ostii flower protein and water in a ratio of 15 mg:1 mL, and perform ultrasonic pretreatment at 60 °C with an ultrasonic power of 700 W for 60 min;

[0052] (2) Add 1 mol / L citric acid solution to the protein solution of Paeonia ostii ‘Feng Dan’ flower after ultrasonic pretreatment to adjust the pH to 5, then add acid protease and ficin (the addition amounts of acid protease and ficin are 6 wt% and 1 wt% of the protein of Paeonia ostii ‘Feng Dan’ flower respectively), heat and hydrolyze at 60 °C for 5 h. After the enzymatic hydrolysis is completed, raise the temperature to 90 °C to inactivate the enzyme for 30 min. Cool the hydrolyzate after enzyme inactivation to 50 °C and add calcium hydroxide powder to adjust the pH to 7. After cooling to room temperature, centrifuge at 7000 rpm for 12 min to obtain the crude peptide solution of Paeonia ostii ‘Feng Dan’ flower. Filter through a membrane to retain the polypeptide filtrate with a molecular weight cut-off of 1000 - 10000 Da. Add trehalose to the polypeptide filtrate (the addition amount of trehalose in the polypeptide filtrate is 1.5 mg / mL), and then freeze-dry to obtain Paeonia ostii ‘Feng Dan’ flower peptide.

[0053] The preparation method of the composite plant flavonoids is as follows: Mix Galinsoga parviflora, Taraxacum mongolicum and Perilla frutescens leaves in a mass ratio of 8:1:1, pulverize, and pass through an 80-mesh sieve to obtain a composite plant powder; add 40 vol% ethanol solution and Tween 80 to the composite plant powder (the dosage ratio of the composite plant powder to the ethanol solution is 1 g:50 mL; the addition amount of Tween 80 is 3 mg / mL), extract at 70 °C with the assistance of ultrasonic wave (ultrasonic power is 300 W) for 2 h, filter, and freeze-dry the filtrate to obtain the composite plant flavonoids.

[0054] Example 4

[0055] An anti-aging composition containing Paeonia ostii ‘Feng Dan’ flower peptide is obtained by uniformly mixing 9 parts of Paeonia ostii ‘Feng Dan’ flower peptide, 4 parts of composite plant flavonoids, 1.5 parts of chitosan oligosaccharide and 0.4 part of sodium ascorbyl phosphate. Among them, the preparation methods of Paeonia ostii ‘Feng Dan’ flower peptide and composite plant flavonoids are the same as those in Example 1.

[0056] Example 5

[0057] An anti-aging composition containing Paeonia ostii ‘Feng Dan’ flower peptide is obtained by uniformly mixing 10 parts of Paeonia ostii ‘Feng Dan’ flower peptide, 5 parts of composite plant flavonoids, 2 parts of chitosan oligosaccharide and 0.5 part of sodium ascorbyl phosphate. Among them, the preparation methods of Paeonia ostii ‘Feng Dan’ flower peptide and composite plant flavonoids are the same as those in Example 1.

[0058] Comparative Example 1

[0059] Same as Example 1, the only difference is that the ultrasonic pretreatment operation in step (1) is omitted during the preparation process of Paeonia ostii ‘Feng Dan’ flower peptide.

[0060] Comparative Example 2

[0061] Same as Example 1, the only difference is that only one enzyme, acid protease (the addition amount of acid protease is 5.5 wt% of the protein of Paeonia ostii ‘Feng Dan’ flower), is added in step (2) during the preparation process of Paeonia ostii ‘Feng Dan’ flower peptide.

[0062] Comparative Example 3

[0063] The same as Example 1, except that, in the preparation process of Danfeng peony flower peptide, the addition of trehalose is omitted before freeze-drying in step (2), and the polypeptide filtrate is directly freeze-dried.

[0064] Comparative Example 4

[0065] The same as Example 1, except that the use of dandelion is omitted in the preparation process of the composite plant flavonoids, and the cyperus rotundus and perilla leaves are mixed and crushed in a mass ratio of 6:1 to obtain the composite plant powder.

[0066] Comparative Example 5

[0067] Same as Example 1, except that the use of chitosan oligosaccharide is omitted.

[0068] Effect Example 1

[0069] Antioxidant performance test

[0070] DPPH (diphenyl picroyl radical) is a stable long-lived free radical. Its ethanol solution is dark purple and has strong absorption near 517nm. When there is a free radical scavenger, the DPPH light absorption is weakened due to its single electron pairing. The degree of fading of DPPH ethanol solution is quantitatively related to the number of electrons it accepts, which can be used to evaluate the ability of antioxidants to scavenge free radicals or the size of antioxidant activity. The greater the inhibition rate, the better the antioxidant effect.

[0071] The compositions prepared in Examples 1-5 and Comparative Examples 1-5 were prepared into 2 mg / mL sample solutions with distilled water, and tested according to the method in Table 1. After reacting at room temperature for 40 minutes, the absorbance was measured at 517 nm. Each sample solution was tested three times, and the results were averaged. DPPH was purchased from Sigma, and the active substance concentration was 60 μg / mL.

[0072] Table 1

[0073] units / mL T T0 C C0 Sample solution 2 2 - - Water - - 2 2 DPPH 2 - 2 - Absolute ethanol - 2 - 2

[0074] The inhibition rate was calculated as follows:

[0075]

[0076] Where T is the sample, T0 is the sample control, C is the blank, and C0 is the blank control.

[0077] The results are shown in Table 2:

[0078] Table 2

[0079]

[0080]

[0081] As can be seen from Table 2, the composition containing Paeonia ostii flower peptide of the present invention has good antioxidant activity, indicating its good anti-aging effect. During the preparation process of Paeonia ostii flower peptide, ultrasonic pretreatment of the protein before hydrolysis, hydrolysis with a composite enzyme (acid protease and ficin), and addition of trehalose before freeze-drying all have a promoting effect on the antioxidant activity of the prepared Paeonia ostii flower peptide, thereby improving the antioxidant activity of the composition. In addition, the raw material composition for extracting composite plant flavonoids and the addition of chitosan oligosaccharide and sodium ascorbyl phosphate also promote the antioxidant activity of the composition.

[0082] The embodiments described above are only descriptions of the preferred embodiments of the present invention, and do not limit the scope of the present invention. Without departing from the design spirit of the present invention, various deformations and improvements made by those of ordinary skill in the art to the technical solutions of the present invention shall fall within the protection scope determined by the claims of the present invention.

Claims

1. An anti-aging composition containing Danfeng Peony Flower Peptide, characterized in that: The raw materials include the following components by weight: 9 parts of Danfeng peony flower peptide, 4 parts of compound plant flavonoids, 1.5 parts of chitosan oligosaccharides and 0.4 parts of sodium ascorbyl phosphate; the compound plant flavonoids are obtained by mixed extraction of cyperus rotundus, dandelion and perilla leaves as raw materials; The preparation method of the Danfeng Peony Flower Peptide is as follows: (1) Mix the peony flower protein with water at a ratio of 10 mg:1 mL, and pre-treat with ultrasound at 650 W power at 55 ° C for 40 min; (2) adding 1 mol / L citric acid solution to the Danfeng peony flower protein solution after ultrasonic pretreatment to adjust the pH to 4.5, then adding acidic protease and ficin, heating and hydrolyzing at 55°C for 4 hours, heating to 85°C for 20 minutes to inactivate the enzyme after the enzymatic hydrolysis, cooling the inactivated hydrolyzate to 50°C, adding calcium hydroxide powder to adjust the pH to 7, cooling to room temperature, and centrifuging at 7000 rpm for 12 minutes to obtain Danfeng peony flower peptide stock solution, filtering the polypeptide filtrate with a molecular weight cutoff of 1000-10000Da through a membrane, adding trehalose to the polypeptide filtrate, and then freeze-drying to obtain Danfeng peony flower peptide; wherein the added amounts of acidic protease and ficin are 5wt% and 0.8wt% of the Danfeng peony flower protein, respectively; and the added amount of trehalose in the polypeptide filtrate is 1 mg / mL; The preparation method of the Danfeng peony flower protein is as follows: crushing Danfeng peony flowers, passing through an 80-mesh sieve, and obtaining Danfeng peony pollen; mixing Danfeng peony pollen with water at a ratio of 1 g:25 mL, adding 1 mol / L NaOH aqueous solution to adjust the pH to 9, and heating and stirring at 55° C. to extract for 5 hours; after the extraction is completed, cooling to room temperature, centrifuging at 7000 rpm for 12 minutes, taking the supernatant, adjusting the pH to 3 with 1 mol / L hydrochloric acid solution, centrifuging at 7000 rpm again for 12 minutes, and taking the precipitate and drying at 45° C. to obtain Danfeng peony flower protein; The preparation method of the composite plant flavonoids is as follows: mixing and crushing cynanchum erythrorhizon, dandelion and perilla leaf in a mass ratio of 7:2:1, and passing through an 80-mesh sieve to obtain composite plant powder; adding 40 vol% ethanol solution and Tween 80 to the composite plant powder, extracting for 1.5 hours at a temperature of 65°C and with the assistance of ultrasound, filtering, and freeze-drying the filtrate to obtain the composite plant flavonoids; wherein the dosage ratio of the composite plant powder to the ethanol solution is 1 g:40 mL, the addition amount of Tween 80 is 2.5 mg / mL; and the ultrasonic power is 250 W; The anti-aging composition containing Danfeng peony flower peptide is used for preparing anti-aging products.

2. A method for preparing an anti-aging composition containing Danfeng Peony Flower Peptide as claimed in claim 1, characterized in that: Take Danfeng peony flower peptide, compound plant flavonoids, chitosan oligosaccharide and sodium ascorbyl phosphate according to mass proportions, and mix them evenly to obtain the product.

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