Preparation method and use of turmeric fiber preparation

Abstract

The present invention provides a preparation method and use of a turmeric fiber preparation, the steps include: filtering after dissolving turmeric fiber powder with deionized water, preparing turmeric water-soluble fiber by alcohol precipitation method; dissolving turmeric water-soluble fiber in deionized water, adding ethanol, adjusting pH value to 4.0-5.0, and stirring to obtain fiber base liquid; dissolving the protein-chitosan nanocomposite loaded with curcumin in anhydrous ethanol to obtain a composite curcumin liquid; adding curcumin liquid dropwise to the fiber base liquid in a stirring state, stirring after adding ethanol, and removing ethanol by rotary evaporation and settling to the original volume with deionized water to obtain a turmeric fiber preparation. The turmeric fiber preparation prepared by the present invention can improve the low solubility and low bioavailability problems of curcumin.

Description

Technical Field

[0001] The invention relates to the technical field of fiber preparations, and in particular to a preparation method and application of a turmeric fiber preparation. Background Art

[0002] Turmeric (Curcuma longa L.), a plant of the ginger family, has always been an integral part of Asian culture and cooking. Curcuminoids are plant polyphenols extracted from the rhizomes of turmeric, mainly represented by curcumin, demethoxycurcumin and bisdemethoxycurcumin. Modern pharmacological studies have shown that curcumin has multiple biological activities such as antioxidant, anti-inflammatory, anti-tumor, anti-proliferative and antibacterial, and is very effective in the prevention and treatment of a variety of diseases. At present, a large number of clinical trials on the anti-tumor activity of curcumin have been widely carried out, mainly focusing on pancreatic cancer and colon cancer. Ulcerative colitis (UC) is a chronic idiopathic inflammatory bowel disease (IBD) of the colon. The disease is mainly characterized by inflammation and ulcerative lesions in the rectal and (sigmoid) colon mucosa and submucosa. In severe cases, it can invade the entire colon and terminal ileum. It is characterized by refractory, recurrent, continuous, high recurrence rate, aggregation and regionality. It requires long-term monitoring and treatment. It has been listed as one of the modern refractory diseases by the World Health Organization (WHO). Studies have shown that a reasonable diet can help relieve UC, because the functional components in the diet can regulate intestinal flora, repair mucosal barriers and enhance immune function. Nevertheless, the actual effect of relieving UC through a regular diet is greatly affected by the patient's dietary preferences, dietary habits and food sources. Therefore, relieving UC by directly supplementing functional components is more in line with practical needs. A large number of studies have shown that specific components such as fiber, polyphenols, functional oils, etc. have a significant effect on UC. The main active ingredient of curcumin is curcumin, which is a common polyphenol with anti-inflammatory, antioxidant, and anti-tumor biological activities. In clinical studies, it has shown positive effects on the treatment, relief, and reduction of recurrence of UC. In addition, turmeric is an essential spice and dye for cooking food. Clinical safety evaluation studies have shown that curcumin does not cause any obvious toxic side effects at a daily dose of up to 8g to 12g. It can be seen that curcumin is a plant polyphenol with multiple health benefits and high safety.

[0003] However, due to its high hydrophobicity and low bioavailability, curcumin's anti-inflammatory, antioxidant, and anti-tumor biological activities are hindered; at the same time, low solubility and low bioavailability have always been the difficulties and key issues that limit the expansion of development and application of curcumin. Curcumin drinks on the market are often made by adding a large amount or multiple surfactants to increase the solubility of curcumin and increase the curcumin content in the drink. This method does not solve the problem of bioavailability well, and it will also introduce a large amount of surfactants into the drink, which is not conducive to the safety and storage stability of the drink. In addition, in order to cover up the special taste of curcumin, which is pungent, bitter, and spicy, many drinks use a lot of flavoring additives, which may not only affect the taste and quality of the drink, but also introduce new safety risks.

[0004] In recent years, with the development of fiber preparation technology, its application in the field of pharmaceutical preparations has become more and more extensive. Fiber preparations have the advantages of large specific surface area, strong adsorption capacity, good biocompatibility, etc., which can improve the solubility and bioavailability of drugs and improve the stability and safety of drugs. Therefore, combining turmeric with fiber preparation technology to develop a new type of turmeric fiber preparation has important research value and practical application prospects. Based on this, the present invention provides a preparation method of turmeric fiber preparation and its use. Summary of the invention

[0005] The purpose of the present invention is to provide a preparation method of a turmeric fiber preparation and use thereof, so as to solve the problems of low solubility and low bioavailability of the above-mentioned curcuminoids when taken orally.

[0006] In one aspect, the present invention provides a turmeric fiber preparation, comprising the steps of:

[0007] (1) Preparing turmeric water-soluble fiber: Dissolving turmeric fiber powder in deionized water and filtering, and preparing turmeric water-soluble fiber by alcohol precipitation method;

[0008] (2) preparing a fiber-based liquid and a curcumin liquid: dissolving turmeric water-soluble fiber in deionized water, adding ethanol, adjusting the pH value to 4.0-5.0, and stirring to obtain a fiber-based liquid; dissolving a protein-chitosan nanocomposite loaded with curcumin in anhydrous ethanol to obtain a composite curcumin liquid;

[0009] (3) Preparation of turmeric fiber preparation: adding the composite curcumin solution dropwise to the fiber base solution in a stirring state, adding ethanol and stirring, removing the ethanol by rotary evaporation, and then fixing the volume to the original volume with deionized water to obtain the turmeric fiber preparation.

[0010] Furthermore, the material-liquid ratio of the turmeric fiber powder to deionized water is 1:(25-35), the temperature of the deionized water is 65-75° C., and the dissolution is carried out under stirring conditions for 25-35 minutes.

[0011] Furthermore, the specific method of the alcohol precipitation method includes: mixing the obtained filtrate with 3-5 times the volume of ethanol and standing for 0.8-1.2 days, centrifuging at 7500-8500 rpm for 12-18 minutes, and then freeze-drying the precipitate.

[0012] Furthermore, in the step (2), ethanol is added to a content of 35-40 vol%, and the reagents for adjusting pH are 0.2 M HCl and NaOH solutions.

[0013] Furthermore, the preparation method of the protein-chitosan nanocomposite loaded with curcumin includes: dissolving lactoferrin and chitosan in two dissociation solutions respectively; dissolving curcumin in ethanol, adjusting the pH value to 5.5, and incubating at 3-5°C for more than 10 hours for complete dissociation; mixing the curcumin ethanol solution with the lactoferrin solution to obtain a mixed solution 1, then mixing the mixed solution 1 and the chitosan solution in equal proportions, and dialyzing to remove urea; after completion, a mixed solution 2 is obtained, and the supernatant is collected by centrifugation.

[0014] Furthermore, the chitosan has a Mw of 200 ku and a deacetylation degree of ≥90%; the dissociation solution comprises 0.5% glacial acetic acid and 6 mol / L urea; the dosage ratio of lactoferrin to the dissociation solution is (200-220) mg: (24-30) mL; the dosage ratio of chitosan to the dissociation solution is (200-220) mg: (24-30) mL; and the dosage ratio of curcumin to ethanol is (500-600) mg: (18-22) mL.

[0015] Furthermore, the pH adjuster is acetic acid.

[0016] Furthermore, the dialysis material is a dialysis tube with a molecular weight of 8.0-14 ku; the dialysis method is to use deionized water for 2-2.5 days at 2-3° C. to remove urea.

[0017] Furthermore, the centrifugation conditions are 8 000 g, 15-25 min.

[0018] Furthermore, the mass ratio of curcumin to turmeric water-soluble fiber in the composite curcumin liquid is 1:20-25.

[0019] Furthermore, the concentration of the compound curcumin solution is 3-7 mg / mL.

[0020] Furthermore, the curcumin has a purity of >95%, and each 10 mg of curcumin comprises 72.22 wt % of curcumin, 17.72 wt % of demethoxycurcumin and 5.26 wt % of bisdemethoxycurcumin.

[0021] Furthermore, after adding ethanol in step (3), the ethanol content reaches 35-40 vol%, and the stirring is continued at 550-650 rpm for 2-4 hours at room temperature.

[0022] Furthermore, the rotary evaporation condition is placed in a 38-45°C water bath, and the rotary evaporation time is 17-23°C.

[0023] Beneficial effects:

[0024] The turmeric fiber preparation provided by the present invention can prevent and treat UC, utilizes the natural interaction force between polyphenols and fibers, selects water-soluble fibers of turmeric itself to encapsulate insoluble curcumin, overcomes the difference in solubility between the fibers and curcumin, constructs a co-dissolving system (40% ethanol) to promote the chemical interaction between the two, and removes ethanol in the system by rotary evaporation, which can assist the encapsulation of curcumin and facilitates the formation of packaged and assembled fiber nanoparticles.

[0025] The protein-chitosan nanocomposite loaded with curcumin has a good ability to relieve the oxidative stress of colon tissue when preparing turmeric fiber preparations. Through the loading of the nanocomposite, the solubility of curcumin is improved, so that its absorption and distribution in the body are more uniform, and its concentration in the colon tissue is increased, which also effectively improves the bioavailability of curcumin; the protein-chitosan nanocomposite has good biocompatibility and targeting, which can achieve targeted delivery to colon tissue, so that curcumin is more concentrated in the colon area to exert its antioxidant effect; in addition, the nanocomposite can achieve sustained release of curcumin, so that the drug is continuously released in the colon area, thereby maintaining a high drug concentration and relieving the oxidative stress response of colon tissue for a long time. At the same time, protein and chitosan themselves may also have certain antioxidant and anti-inflammatory effects, and work together with curcumin to further enhance the ability to relieve oxidative stress of colon tissue. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 This is a DAI score curve in Experimental Example 1 of the present invention;

[0027] Figure 2 This is a graph corresponding to the colon morphology score in Experimental Example 1 of the present invention;

[0028] Figure 3 This is the HE staining image of the colon morphology in Experimental Example 1 of the present invention;

[0029] Figure 4 This is a graph of D-lactic acid concentration in Experimental Example 1 of the present invention;

[0030] Figure 5 This is a graph of GSH concentration in Experimental Example 1 of the present invention;

[0031] Figure 6 This is a graph of SOD concentration in Experimental Example 1 of the present invention;

[0032] Figure 7 This is a graph of IL-1β concentration in Experimental Example 1 of the present invention;

[0033] Figure 8 This is a graph of IL-6 concentration in Experimental Example 1 of the present invention;

[0034] Fig. 9 This is a graph of IL-10 concentration in Experimental Example 1 of the present invention;

[0035] Fig.10 This is a graph showing the concentration of TNF-α in Experimental Example 1 of the present invention. DETAILED DESCRIPTION

[0036] The technical solution of the present invention is described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0037] The embodiments of the present invention will be described in detail below in conjunction with examples. However, those skilled in the art will understand that the following examples are only used to illustrate the present invention and should not be construed as limiting the scope of the present invention. The specific conditions not specified in the examples are carried out according to conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used without indicating the manufacturer are all conventional products that can be purchased commercially.

[0038] Turmeric fiber powder was purchased from Shaanxi Lvlai Biotechnology Co., Ltd., product batch number: LL211012-1.

[0039] Example 1

[0040] This embodiment provides a method for preparing a turmeric fiber preparation, the steps comprising:

[0041] (1) Preparation of curcumin-loaded protein-chitosan nanocomposites: 210 mg lactoferrin and 210 mg chitosan with a Mw of 200 ku and a deacetylation degree of ≥90% were dissolved in two 27 mL dissociation solutions containing 0.5% glacial acetic acid and 6 mol / L urea, respectively; the final pH value was adjusted to 5.5 with acetic acid, and the mixture was incubated at 4°C for 12 h for complete dissociation; 550 mg curcumin was dissolved in 20 mL ethanol to obtain a curcumin ethanol solution; the curcumin ethanol solution was mixed with the lactoferrin solution to obtain a mixed solution 1; the mixed solution 1 and the chitosan solution were mixed in equal proportions, and dialyzed with deionized water at 2.5°C for 2 days using a dialysis tube with a molecular weight of 10 ku to remove urea; after the dialysis was completed, a mixed solution 2 was obtained, which was centrifuged at 8,000 g for 20 min, and the supernatant was collected;

[0042] Among them, 10 mg of curcumin (purity>95%) includes 72.22 wt % of curcumin, 17.72 wt % of demethoxycurcumin and 5.26 wt % of bisdemethoxycurcumin.

[0043] (2) Preparation of composite curcumin solution: dissolving the protein-chitosan nanocomposite loaded with curcumin in anhydrous ethanol to obtain a composite curcumin solution, wherein the concentration of the composite curcumin solution is 5 mg / mL;

[0044] (3) Preparation of turmeric water-soluble fiber: turmeric fiber powder was placed in 70°C water with a solid-liquid ratio of 1:30, stirred for 30 minutes, and filtered; the obtained filtrate was mixed with 4 volumes of ethanol and allowed to stand for 1 day, centrifuged at 8000 rpm for 15 minutes, and the precipitate was freeze-dried to obtain turmeric water-soluble fiber;

[0045] (4) Preparation of fiber-based liquid: dissolving the prepared turmeric water-soluble fiber in pure water, adding ethanol to make the ethanol content reach 40 vol%, adjusting the pH value to 4 with 0.2 M HCl and NaOH solution, stirring, and obtaining a fiber-based liquid;

[0046] Among them, the mass ratio of curcumin to turmeric water-soluble fiber in the composite curcumin solution is 1:30;

[0047] (5) The composite curcumin liquid was added dropwise to the fiber base liquid in a stirring state, and ethanol was then added dropwise to make the ethanol content reach 40 vol%, and the mixture was stirred at 600 rpm for 3 h at room temperature, and then placed in a 40° C. water bath for rotary evaporation for 20 min to remove the ethanol, and the volume was adjusted to the original volume with water to obtain a turmeric fiber preparation.

[0048] Comparative Example 1

[0049] The steps of preparing the composite curcumin in this comparative example include:

[0050] (1) Preparation of protein-chitosan nanocomposites loaded with curcumin: 210 mg lactoferrin and 210 mg chitosan with a Mw of 200 ku and a deacetylation degree of ≥90% were dissolved in two 27 mL dissociation solutions containing 0.5% glacial acetic acid and 6 mol / L urea, respectively; the final pH value was adjusted to 5.5 with acetic acid, and the mixture was incubated at 4°C for 12 h for complete dissociation; 550 mg curcumin was dissolved in 20 mL ethanol to obtain a curcumin ethanol solution; the curcumin ethanol solution was mixed with the lactoferrin solution to obtain a mixed solution 1; the mixed solution 1 and the chitosan solution were mixed in equal proportions, and dialyzed with deionized water at 2.5°C for 2 days using a dialysis tube with a molecular weight of 10 ku to remove urea; after the dialysis was completed, a mixed solution 2 was obtained, which was centrifuged at 8,000 g for 20 min, and the supernatant was collected;

[0051] Among them, 10 mg of curcumin (purity>95%) includes 72.22 wt % of curcumin, 17.72 wt % of demethoxycurcumin and 5.26 wt % of bisdemethoxycurcumin.

[0052] (2) Preparation of composite curcumin solution: The protein-chitosan nanocomposite loaded with curcumin was dissolved in anhydrous ethanol to obtain a composite curcumin solution, wherein the concentration of the composite curcumin solution was 5 mg / mL.

[0053] Comparative Example 2

[0054] The steps of preparing turmeric water-soluble fiber in this comparative example include: placing turmeric fiber powder in 70° C. water with a solid-liquid ratio of 1:30, stirring for 30 minutes, and filtering; mixing the obtained filtrate with 4 times the volume of ethanol and standing for 1 day, centrifuging at 8000 rpm for 15 minutes, and freeze-drying the precipitate to obtain turmeric water-soluble fiber.

[0055] In order to further illustrate the performance and beneficial effects of the fiber preparation for preventing and treating UC provided by the present invention, the following experiments were conducted:

[0056] Test Example 1

[0057] Acute UC modeling was performed by freely giving 3% DSS to drink, and mesalazine (5-ASA) was used as a positive control drug. After 7 days of adaptive feeding, 48 male SPF BALB / c mice were randomly divided into six groups: control group, model group, positive drug group, compound curcumin group (prepared in comparative example 1), turmeric water-soluble fiber group (prepared in comparative example 2) and turmeric fiber preparation group (prepared in Example 1). Starting from the first day, except for the control group mice, the remaining groups were gavaged with the corresponding test drugs, once a day, and freely drank 3% DSS solution to induce UC model for 9 consecutive days; the control group was gavaged and drank sterile deionized water every day. The mice were weighed every day during the experiment, and the disease activity index (DAI) was scored. On the 10th day of the experiment, the mice were killed and blood was collected after eyeball removal. The length of the colon segment was measured and histological damage was scored. Part of the colon was fixed in 4% paraformaldehyde solution, embedded in paraffin and sectioned. Hematoxylin andeosin staining (HE) was used to observe the pathological changes of colon mucosa; biochemical analysis was used to detect superoxide dismutase (SOD) and glutathione (GSH) in colon tissue; enzyme-linked immunosorbent assay was used to determine the levels of interleukin-1β (IL-1β), interleukin-6 (IL-6), interleukin-10 (IL-10) and tumor necrosis factor-α (TNF-α) in plasma; and the content of D-lactic acid (D(-)-lactic acid) was detected by microassay. The results are shown in the following table:

[0058] Table 1 DAI score table (mean ± standard deviation, n = 8)

[0059]

[0060]

[0061] Table 2 Macroscopic morphological scores of colon tissue (mean ± SD, n = 8)

[0062]

[0063]

[0064] Figure 1 This is a DAI score curve in Experimental Example 1 of the present invention; Figure 2 This is a graph corresponding to the colon morphology score in Experimental Example 1 of the present invention; Figure 3 This is the HE staining image of the colon morphology in Experimental Example 1 of the present invention; Figure 4 This is a graph of D-lactic acid concentration in Experimental Example 1 of the present invention; Figure 5 This is a graph of GSH concentration in Experimental Example 1 of the present invention; Figure 6 This is a graph of SOD concentration in Experimental Example 1 of the present invention; Figure 7 This is a graph of IL-1β concentration in Experimental Example 1 of the present invention;

[0065] Figure 8 This is a graph of IL-6 concentration in Experimental Example 1 of the present invention; Fig. 9 This is a graph of IL-10 concentration in Experimental Example 1 of the present invention; Fig.10 This is a graph showing the concentration of TNF-α in Experimental Example 1 of the present invention.

[0066] From Table 1 and Figure 1 It can be seen that the DAI score of the turmeric fiber preparation group was lower than that of the model group, the compound curcumin group and the turmeric water-soluble fiber group. The DAI score can judge the severity of colitis symptoms. It can be seen that the turmeric fiber preparation has a strong effect in relieving colon inflammation. Figure 2 The colon morphology score of the turmeric fiber preparation group was lower than that of the model group, compound curcumin group and turmeric water-soluble fiber group. The colon morphology can directly determine the severity of colitis symptoms, which shows that the turmeric fiber preparation has a good protective effect on colon morphology.

[0067] Depend on Figure 3 It was found that compared with the control group, the histopathological structure and neutrophil infiltration in the turmeric fiber preparation group and the positive drug group did not change significantly, while the number of goblet cells increased significantly and was arranged in an orderly manner. There were more inflammatory infiltrations and intestinal wall structural damage in the model group, compound curcumin group and turmeric water-soluble fiber group. It can be seen that the turmeric fiber preparation has a protective effect on the intestine and reduces DSS-induced colon tissue damage.

[0068] Depend on Figure 4 It was found that the D-lactic acid content in the turmeric fiber preparation group was lower than that in the model group, the compound curcumin group and the turmeric water-soluble fiber group. When the intestinal mucosal permeability increases, a large amount of D-lactic acid produced by bacteria in the intestine enters the blood through the damaged mucosa, increasing the plasma D-lactic acid level. It can be seen that the turmeric fiber preparation has a protective effect on the intestine.

[0069] Depend on Figure 5 It can be seen that the GSH content of the turmeric fiber preparation group was higher than that of the model group, the compound curcumin group and the turmeric water-soluble fiber group. GSH can help the body maintain normal immune system function and has antioxidant effects. The oxidative stress level of each group of mice can be evaluated. It can be seen that the turmeric fiber preparation has a good ability to alleviate the oxidative stress of colon tissue.

[0070] Depend on Figure 6It was found that the SOD content in the turmeric fiber preparation group was higher than that in the model group, the compound curcumin group and the turmeric water-soluble fiber group. SOD is an antioxidant enzyme that can catalyze the dismutation of superoxide anion radicals to generate oxygen and hydrogen peroxide. It plays a vital role in the balance of oxidation and antioxidant activity in the body and can evaluate the oxidative stress level of mice in each group. It can be seen that the turmeric fiber preparation has a good ability to alleviate the oxidative stress of colon tissue.

[0071] Depend on Figure 7 It can be seen that the IL-1β content in the turmeric fiber preparation group was lower than that in the model group, the compound curcumin group and the turmeric water-soluble fiber group. The large release of IL-1β directly led to the development of intestinal inflammation. It can be seen that the turmeric fiber preparation has a better ability to reduce this inflammatory factor and relieve intestinal inflammation.

[0072] Depend on Figure 8 It can be seen that the IL-6 content in the turmeric fiber preparation group was lower than that in the model group, the compound curcumin group and the turmeric water-soluble fiber group. The large release of IL-6 directly led to the development of intestinal inflammation. It can be seen that the turmeric fiber preparation has a better ability to reduce this inflammatory factor and relieve intestinal inflammation.

[0073] Depend on Fig. 9 It can be seen that the IL-10 content of the turmeric fiber preparation group was higher than that of the model group, the compound curcumin group and the turmeric water-soluble fiber group. The lack of IL-10 will aggravate the development of colon inflammation. It can be seen that the turmeric fiber preparation has a better ability to increase this anti-inflammatory factor and relieve intestinal inflammation.

[0074] Depend on Fig.10 It can be seen that the TNF-α content in the turmeric fiber preparation group was lower than that in the model group, the compound curcumin group and the turmeric water-soluble fiber group. The large release of TNF-α directly led to the development of intestinal inflammation. It can be seen that the turmeric fiber preparation has a better ability to reduce this inflammatory factor and relieve intestinal inflammation.

[0075] In summary, compared with the model group, the turmeric fiber preparation effectively alleviated the DAI score level, blood and tissue inflammatory protein levels (TNF-α, IL-6, IL-10 and IL-1β), and was stronger than the single use of free curcumin and turmeric water-soluble fiber; HE staining also showed that the turmeric fiber preparation had a good effect on maintaining the colon morphology and effectively downregulated the D-lactic acid level in plasma; at the same time, the SOD and GSH contents were high, and compared with the model group, it significantly increased the antioxidant effect on UC, and was stronger than the single use of free curcumin and turmeric water-soluble fiber.

[0076] Example 2

[0077] This embodiment provides a method for preparing a turmeric fiber preparation, the steps comprising:

[0078] (1) Preparation of protein-chitosan nanocomposites loaded with curcumin: 200 mg lactoferrin and 210 mg chitosan with a Mw=200 ku and a deacetylation degree of ≥90% were dissolved in two 24 mL dissociation solutions containing 0.5% glacial acetic acid and 6 mol / L urea, respectively; the final pH value was adjusted to 5.5 with acetic acid, and the mixture was incubated at 4°C for 10 h for complete dissociation; 500 mg curcumin was dissolved in 18 mL ethanol to obtain a curcumin ethanol solution; the curcumin ethanol solution was mixed with the lactoferrin solution to obtain a mixed solution 1; the mixed solution 1 and the chitosan solution were mixed in equal proportions, and dialyzed with deionized water at 2.5°C for 2 days using a dialysis tube with a molecular weight of 10 ku to remove urea; after the dialysis was completed, a mixed solution 2 was obtained, which was centrifuged at 8,000 g for 15 min, and the supernatant was collected;

[0079] Among them, 10 mg of curcumin (purity>95%) includes 72.22 wt % of curcumin, 17.72 wt % of demethoxycurcumin and 5.26 wt % of bisdemethoxycurcumin.

[0080] (2) Preparation of composite curcumin solution: dissolving the protein-chitosan nanocomposite loaded with curcumin in anhydrous ethanol to obtain a composite curcumin solution; the concentration of the composite curcumin solution is 4 mg / mL;

[0081] (3) Preparation of turmeric water-soluble fiber: turmeric fiber powder was placed in 70°C water with a solid-liquid ratio of 1:30, stirred for 30 minutes, and filtered; the obtained filtrate was mixed with 4 volumes of ethanol and allowed to stand for 1 day, centrifuged at 8000 rpm for 15 minutes, and the precipitate was freeze-dried to obtain turmeric water-soluble fiber;

[0082] (4) Preparation of fiber-based liquid: dissolving the prepared turmeric water-soluble fiber in pure water, adding ethanol to make the ethanol content reach 40 vol%, adjusting the pH value to 4 with 0.2 M HCl and NaOH solution, stirring, and obtaining a fiber-based liquid;

[0083] Among them, the mass ratio of curcumin to turmeric water-soluble fiber in the composite curcumin solution is 1:30;

[0084] (5) The composite curcumin liquid was added dropwise to the fiber base liquid in a stirring state, and ethanol was then added dropwise to make the ethanol content reach 40 vol%, and the mixture was stirred at 600 rpm for 3 h at room temperature, and then placed in a 40° C. water bath for rotary evaporation for 20 min to remove the ethanol, and the volume was adjusted to the original volume with water to obtain a turmeric fiber preparation.

[0085] Example 3

[0086] This embodiment provides a method for preparing a turmeric fiber preparation, the steps comprising:

[0087] (1) Preparation of protein-chitosan nanocomposites loaded with curcumin: 220 mg lactoferrin and 210 mg chitosan with Mw=200 ku and a deacetylation degree of ≥90% were dissolved in two 30 mL dissociation solutions containing 0.5% glacial acetic acid and 6 mol / L urea, respectively; the final pH value was adjusted to 5.5 with acetic acid, and the mixture was incubated at 4°C for 12 h for complete dissociation; 600 mg curcumin was dissolved in 22 mL ethanol to obtain a curcumin ethanol solution; the curcumin ethanol solution was mixed with the lactoferrin solution to obtain a mixed solution 1; the mixed solution 1 and the chitosan solution were mixed in equal proportions, and dialyzed with deionized water at 2.5°C for 2 days using a dialysis tube with a molecular weight of 10 ku to remove urea; after the dialysis was completed, a mixed solution 2 was obtained, which was centrifuged at 8,000 g for 25 min, and the supernatant was collected;

[0088] Among them, 10 mg of curcumin (purity>95%) includes 72.22 wt % of curcumin, 17.72 wt % of demethoxycurcumin and 5.26 wt % of bisdemethoxycurcumin.

[0089] (2) Preparation of composite curcumin solution: dissolving the protein-chitosan nanocomposite loaded with curcumin in anhydrous ethanol to obtain a composite curcumin solution; the concentration of the composite curcumin solution is 7 mg / mL;

[0090] (3) Preparation of turmeric water-soluble fiber: turmeric fiber powder was placed in 70°C water with a solid-liquid ratio of 1:30, stirred for 30 minutes, and filtered; the obtained filtrate was mixed with 4 volumes of ethanol and allowed to stand for 1 day, centrifuged at 8000 rpm for 15 minutes, and the precipitate was freeze-dried to obtain turmeric water-soluble fiber;

[0091] (4) Preparation of fiber-based liquid: dissolving the prepared turmeric water-soluble fiber in pure water, adding ethanol to make the ethanol content reach 40 vol%, adjusting the pH value to 4 with 0.2 M HCl and NaOH solution, stirring, and obtaining a fiber-based liquid;

[0092] Among them, the mass ratio of curcumin to turmeric water-soluble fiber in the composite curcumin solution is 1:30;

[0093] (5) The composite curcumin liquid was added dropwise to the fiber base liquid in a stirring state, and ethanol was then added dropwise to make the ethanol content reach 40 vol%, and the mixture was stirred at 600 rpm for 3 h at room temperature, and then placed in a 40° C. water bath for rotary evaporation for 20 min to remove the ethanol, and the volume was adjusted to the original volume with water to obtain a turmeric fiber preparation.

[0094] Example 4

[0095] This embodiment provides a method for preparing a turmeric fiber preparation, the steps comprising:

[0096] (1) Preparation of protein-chitosan nanocomposites loaded with curcumin: 200 mg lactoferrin and 210 mg chitosan with Mw=200 ku and a deacetylation degree of ≥90% were dissolved in two 25 mL dissociation solutions containing 0.5% glacial acetic acid and 6 mol / L urea, respectively; the final pH value was adjusted to 5.5 with acetic acid, and the mixture was incubated at 4°C for 12 h for complete dissociation; 560 mg curcumin was dissolved in 21 mL ethanol to obtain a curcumin ethanol solution; the curcumin ethanol solution was mixed with the lactoferrin solution to obtain a mixed solution 1; the mixed solution 1 and the chitosan solution were mixed in equal proportions, and dialyzed with deionized water at 2.5°C for 2 days using a dialysis tube with a molecular weight of 10 ku to remove urea; after the dialysis was completed, a mixed solution 2 was obtained, which was centrifuged at 8,000 g for 20 min, and the supernatant was collected;

[0097] Among them, 10 mg of curcumin (purity>95%) includes 72.22 wt % of curcumin, 17.72 wt % of demethoxycurcumin and 5.26 wt % of bisdemethoxycurcumin.

[0098] (2) Preparation of composite curcumin solution: dissolving the protein-chitosan nanocomposite loaded with curcumin in anhydrous ethanol to obtain a composite curcumin solution; the concentration of the composite curcumin solution is 6 mg / mL;

[0099] (3) Preparation of turmeric water-soluble fiber: turmeric fiber powder was placed in 70°C water with a solid-liquid ratio of 1:30, stirred for 30 minutes, and filtered; the obtained filtrate was mixed with 4 volumes of ethanol and allowed to stand for 1 day, centrifuged at 8000 rpm for 15 minutes, and the precipitate was freeze-dried to obtain turmeric water-soluble fiber;

[0100] (4) Preparation of fiber-based liquid: dissolving the prepared turmeric water-soluble fiber in pure water, adding ethanol to make the ethanol content reach 40 vol%, adjusting the pH value to 4 with 0.2 M HCl and NaOH solution, stirring, and obtaining a fiber-based liquid;

[0101] Among them, the mass ratio of curcumin to turmeric water-soluble fiber in the composite curcumin solution is 1:30;

[0102] (5) The composite curcumin liquid was added dropwise to the fiber base liquid in a stirring state, and ethanol was then added dropwise to make the ethanol content reach 40 vol%, and the mixture was stirred at 600 rpm for 3 h at room temperature, and then placed in a 40° C. water bath for rotary evaporation for 20 min to remove the ethanol, and the volume was adjusted to the original volume with water to obtain a turmeric fiber preparation.

[0103] Test Example 2

[0104] According to the above test method, Examples 2-3 were tested, and the data of Example 1 was used as a comparison. The results are shown in Table 3 below:

[0105] Table 3 DAI score table (mean ± standard deviation, n = 8)

[0106]

[0107]

[0108] Finally, it should be noted that the above embodiments are only used to illustrate the present invention and are not intended to limit the technical solutions described in the present invention. Those skilled in the art should understand that the present invention can still be modified or replaced by equivalents. All technical solutions and improvements that do not depart from the spirit and scope of the present invention should be included in the scope of the claims of the present invention.

Claims

1. A method for preparing a turmeric fiber preparation, characterized in that the steps include: (1) Preparing turmeric water-soluble fiber: Dissolving turmeric fiber powder in deionized water and filtering, and preparing turmeric water-soluble fiber by alcohol precipitation method; (2) preparing a fiber-based liquid and a curcumin liquid: dissolving turmeric water-soluble fiber in deionized water, adding ethanol, adjusting the pH value to 4.0-5.0, and stirring to obtain a fiber-based liquid; dissolving a protein-chitosan nanocomposite loaded with curcumin in anhydrous ethanol to obtain a composite curcumin liquid; (3) Preparing a turmeric fiber preparation: adding the composite curcumin solution dropwise to the fiber base solution in a stirring state, adding ethanol, stirring, rotary evaporating to remove the ethanol, and then diluting to the original volume with deionized water to obtain a turmeric fiber preparation; The preparation method of the protein-chitosan nanocomposite loaded with curcumin comprises: dissolving lactoferrin and chitosan in two dissociation solutions respectively; dissolving curcumin in ethanol, adjusting the pH value to 5.5, and incubating at 3-5°C for more than 10 hours for complete dissociation; mixing the curcumin ethanol solution with the lactoferrin solution to obtain a mixed solution 1, then mixing the mixed solution 1 and the chitosan solution in equal proportions, and dialyzing to remove urea; after completion, a mixed solution 2 is obtained, and the supernatant is collected by centrifugation.

2. The method for preparing a turmeric fiber preparation according to claim 1, characterized in that: The material-liquid ratio of the turmeric fiber powder to deionized water is 1:(25-35), the temperature of the deionized water is 65-75° C., and the dissolution is carried out under stirring conditions for 25-35 minutes.

3. The method for preparing a turmeric fiber preparation according to claim 1, characterized in that: The specific method of the alcohol precipitation method includes: mixing the obtained filtrate with 3-5 times the volume of ethanol and standing for 0.8-1.2 days, centrifuging at 7500-8500 rpm for 12-18 minutes, and then freeze-drying the precipitate.

4. The method for preparing a turmeric fiber preparation according to claim 1, characterized in that: In the step (2), ethanol is added to a content of 35-40 vol%, and the reagents for adjusting pH are 0.2 M HCl and NaOH solutions.

5. The method for preparing a turmeric fiber preparation according to claim 1, characterized in that: The chitosan has a Mw of 200 ku and a deacetylation degree of ≥90%; the dissociation solution comprises 0.5% glacial acetic acid and 6 mol / L urea; the dosage ratio of lactoferrin to the dissociation solution is (200-220) mg: (24-30) mL; the dosage ratio of chitosan to the dissociation solution is (200-220) mg: (24-30) mL; and the dosage ratio of curcumin to ethanol is (500-600) mg: (18-22) mL.

6. The method for preparing a turmeric fiber preparation according to claim 1, characterized in that: The curcumin has a purity of >95%, and each 10 mg of curcumin comprises 72.22 wt % of curcumin, 17.72 wt % of demethoxycurcumin and 5.26 wt % of bisdemethoxycurcumin; the concentration of the composite curcumin liquid is 3-7 mg / mL; and the mass ratio of curcumin to turmeric water-soluble fiber in the composite curcumin liquid is 1:20-25.

7. The method for preparing a turmeric fiber preparation according to claim 1, characterized in that: After adding ethanol in the step (3), the ethanol content reaches 35-40 vol%, and the stirring is continued at 550-650 rpm for 2-4 hours at room temperature.

8. The method for preparing a turmeric fiber preparation according to claim 1, characterized in that: The rotary evaporation conditions are as follows: placing the mixture in a 38-45°C water bath, and the rotary evaporation time is 17-23°C.

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