An oyster peptide powder composition, its preparation method and applications

Abstract

The present invention discloses an oyster peptide powder composition, a preparation method and an application thereof. The oyster peptide powder composition comprises the following components in parts by weight: 20-40 parts of oyster peptide powder, 8-15 parts of silybum marianum extract, 2-6 parts of ampelopsis grossedentata extract and 2-6 parts of licorice extract. Each component in the oyster peptide powder composition of the present invention acts synergistically with each other, has a good effect on relieving alcoholic liver injury, can significantly reduce the contents of TNF-α and MDA in the liver tissue of mice, reduce the activities of ALT and AST in the serum, improve the activity of SOD in the liver tissue, and play an obvious protective role on liver injury, and has a wide application prospect.

Description

Technical Field

[0001] The present invention relates to the field of biotechnology, and particularly relates to an oyster peptide powder composition, a preparation method thereof, and an application thereof. Background Art

[0002] Alcoholic liver disease (ALD) is a liver disease caused by long-term and excessive alcohol consumption. In the initial stage, it usually presents as fatty liver, and can further develop into alcoholic hepatitis, liver fibrosis, and cirrhosis, and may even cause the occurrence of liver cancer. The main clinical features are nausea, vomiting, jaundice, possible liver enlargement and tenderness, and can be complicated by liver failure and upper gastrointestinal bleeding, etc. For the treatment of ALD, there is currently no successful method, and there is no specific drug for ALD clinically. Usually, the treatment of alcoholic fatty liver is mainly based on abstinence from alcohol and reasonable diet, and clinically, antioxidant drugs such as polyene phosphatidylcholine (PPC) and silymarin, and lipid-lowering drugs such as pravastatin, simvastatin, and fluvastatin are orally administered in combination, but this cannot achieve a radical cure effect, and side effects such as abdominal discomfort and allergies will occur during the use process.

[0003] According to existing research, a variety of food-derived natural products have a protective effect on ALD, and their main bioactive components are flavonoids, polyphenols, saponins, polysaccharides, terpene compounds, and alkaloids, etc.

[0004] Patent CN112675283B discloses an oyster peptide powder with liver protection function and a preparation method thereof, which includes the following raw materials and their weight parts: 10-20 parts of oyster peptide, 1-4 parts of seabuckthorn extract, 2-5 parts of nitidine chloride, 5-8 parts of emulsifier, and 0.4-1 part of plant composite oil. The oyster peptide powder of the present invention has a significant liver protection function and can significantly reduce the increase of triglyceride (TG) and malondialdehyde (MDA) in the liver tissue of ethanol-induced liver injury mice. Although the oyster peptide powder of this patent has a certain liver protection effect, the effect is limited.

[0005] Patent CN113712205B discloses an oyster peptide powder composition, a preparation method thereof, and an application thereof. The composition includes at least two of curcumin, pueraria extract, and hovenia dulcis extract, and oyster peptide powder or oyster oligopeptide. The composition has a significant neuroprotective function, an immune-enhancing effect, and a significant anti-alcohol liver protection effect.

[0006] Oysters, commonly known as sea oysters and oysters, are a type of shellfish that are abundant and can be extensively cultured along the coasts of our country. There are even more abroad. Oysters are traditional tonics. "Compendium of Materia Medica" records: "Oysters can treat deficiency and damage, strengthen yang, detoxify, supplement the qi and blood of both men and women, make the skin delicate, and prevent aging." A large amount of clinical data confirms that oysters have the functions of increasing the serum testosterone level in men, regulating blood lipids, inhibiting platelet aggregation, improving hyperglycemia symptoms, enhancing human immunity, and promoting metabolism, and also have a certain effect on anti-cancer and preventing the spread of cancer cells.

[0007] Silybum marianum, the dried and mature fruit of the Silybum marianum plant of the Compositae family, is bitter and cool in nature, and has the effects of clearing heat and detoxifying, soothing the liver and gallbladder. "National Compilation of Chinese Herbal Medicines" records that Silybum marianum can resist liver damage, atherosclerosis, cerebral ischemia, inhibit platelet aggregation, and has anti-tumor effects.

[0008] Therefore, it is very necessary to further explore and utilize the nutritional and medicinal values of oysters and Silybum marianum, and develop liver-protecting products with more significant effects and greater safety. Summary of the Invention

[0009] The purpose of the present invention is to provide an oyster peptide powder composition, its preparation method and application. By preparing oyster peptide powder and Silybum marianum extract, and compounding them with Ampelopsis grossedentata extract and licorice extract, a composition with significant improvement in alcoholic liver injury is obtained, which can be applied to medicines.

[0010] To this end, the present invention adopts the following technical solutions:

[0011] In the first aspect of the present invention, an oyster peptide powder composition is provided, which comprises the following components in parts by weight: 20-40 parts of oyster peptide powder, 8-15 parts of Silybum marianum extract, 2-6 parts of Ampelopsis grossedentata extract, and 2-6 parts of licorice extract.

[0012] Further, the preparation method of the oyster peptide powder includes the following steps:

[0013] S1, Take the washed oyster meat, crush it, mix it with water, add protease, adjust the pH, carry out enzymatic hydrolysis reaction, inactivate the enzyme, and centrifuge or filter to obtain the enzymatic hydrolysis filtrate;

[0014] S2, Add water to the filter residue and homogenize it with a high-pressure homogenizer, sterilize it at 121-135 °C for 15-25 min to obtain a homogenized slurry, and cool it for later use;

[0015] S3, Add the lactic acid bacteria strain seed liquid to the above homogenized slurry, ferment it at 30-45 °C for 6-72 h to obtain a fermentation broth;

[0016] S4, Mix the enzymatic hydrolysis filtrate and the fermentation broth, concentrate, and spray dry to obtain the oyster peptide powder.

[0017] As a preferred technical solution, the protease includes at least one selected from the group consisting of alkaline protease, flavor protease, animal protease, papain, neutral protease, bromelain, trypsin, acid protease, and pepsin.

[0018] As a preferred technical solution, the addition amount of the enzyme is 0.15 - 5.0 wt%.

[0019] As a preferred technical solution, the lactic acid bacteria are at least one of Lactobacillus reuteri, Lactobacillus plantarum, and Lactobacillus bulgaricus.

[0020] As a preferred technical solution, the inoculation amounts of the lactic acid bacteria strain seed liquid are respectively 1 - 5 v / v%, and the bacteria content of the strain seed liquid is 10 8 -10 9 cfu / mL.

[0021] Furthermore, the preparation method of the silymarin extract includes the following steps:

[0022] S1, cleaning and drying the silymarin fruits, removing oil by pressing method, and pulping with 5 - 10 times of water;

[0023] S2, adding a composite enzyme composed of cellulase and pectinase to the above-mentioned slurry, controlling the temperature at 45 - 55°C, pH at 4.5 - 5.5, and pressure at 120 - 350 mpa, and performing enzymatic hydrolysis to obtain an enzymatic hydrolysate;

[0024] S3, extracting the above-mentioned enzymatic hydrolysate with absolute ethanol at room temperature for 1 - 3 times, combining the multiple extraction liquids, concentrating, removing the solvent, and performing spray drying to obtain the silymarin extract.

[0025] As a preferred technical solution, the addition amount of the composite enzyme is 0.2 - 5.0 wt%.

[0026] As a preferred technical solution, the weight ratio of the cellulase to the pectinase is 2 - 5:1, and the enzymatic hydrolysis time is 30 - 70 min.

[0027] In the second aspect of the present invention, a preparation method of an oyster peptide powder composition is provided, and the preparation method includes: weighing oyster peptide powder, silymarin extract, tengcha extract, and licorice extract according to the ratio, and mixing them evenly to obtain the product.

[0028] In the third aspect of the present invention, an application of the oyster peptide powder composition in the preparation of a liver protection product is provided.

[0029] Furthermore, the product is a medicine, and the product further includes pharmaceutically acceptable excipients.

[0030] Further, the pharmaceutically acceptable excipients include at least one of a carrier, an excipient, and a diluent.

[0031] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0032] The present invention uses a compound of oyster peptide powder, silybum marianum extract, ampelopsis grossedentata extract, and glycyrrhiza glabra extract to obtain an oyster peptide powder composition. The four components act in coordination to enhance the effect of improving liver injury.

[0033] The present invention combines protease hydrolysis of oyster meat and lactic acid bacteria fermentation to prepare oyster peptide powder with high nutritional value.

[0034] The present invention uses a composite enzyme of cellulase and pectinase to enzymatically hydrolyze silybum marianum fruits and extracts them multiple times with ethanol as a solvent to obtain a silybum marianum extract with a high content of silymarin.

[0035] Animal experiments have confirmed that the oyster peptide powder composition of the present invention can significantly reduce the contents of TNF-α and MDA in the liver tissues of mice, reduce the activities of ALT and AST in the serum, and increase the activity of SOD in the liver tissues, having a good effect of relieving alcoholic liver injury and playing an obvious protective role on liver injury. Specific Embodiments

[0036] The following further describes the specific embodiments of the technical solution of this specification through specific examples. These examples are for a detailed description of the technical solution and not for limiting the technical solution. Based on the examples in this specification, all other examples obtained by those of ordinary skill in the art without creative efforts belong to the scope protected by this specification. Unless otherwise specified, the reagents, methods, and equipment used in the present invention are conventional reagents, methods, and equipment in the technical field. The reagents and materials used in the following examples are all commercially available.

[0037] In a first aspect, the present invention provides an oyster peptide powder composition, comprising the following components in parts by weight: 20-40 parts of oyster peptide powder, 8-15 parts of silybum marianum extract, 2-6 parts of ampelopsis grossedentata extract, and 2-6 parts of glycyrrhiza glabra extract.

[0038] Further, the preparation method of the oyster peptide powder includes the following steps:

[0039] S1. Take the washed oyster meat, crush it, mix it with water, add protease, adjust the pH, carry out an enzymatic hydrolysis reaction, inactivate the enzyme, and centrifuge or filter to obtain an enzymatically hydrolyzed filtrate;

[0040] S2. Add water to the filter residue and homogenize it with a high-pressure homogenizer, sterilize it at 121-135 °C for 15-25 min to obtain a homogenized solution, and cool it for later use;

[0041] S3. Add the lactic acid bacteria strain seed liquid to the above homogenate and ferment at 30 - 45 °C for 6 - 72 h to obtain a fermentation broth.

[0042] S4. Mix the enzymolysis filtrate and the fermentation broth, concentrate, and spray dry to obtain the oyster peptide powder.

[0043] As a preferred technical solution, the protease includes at least one selected from alkaline protease, flavor protease, animal protease, papain, neutral protease, bromelain, trypsin, acidic protease, and pepsin.

[0044] As a preferred technical solution, the addition amount of the enzyme is 0.15 - 5.0 wt%.

[0045] As a preferred technical solution, the lactic acid bacteria are at least one of Lactobacillus reuteri, Lactobacillus plantarum, and Lactobacillus bulgaricus.

[0046] As a preferred technical solution, the inoculation amounts of the lactic acid bacteria strain seed liquid are 1 - 5 v / v%, and the bacteria content of the strain seed liquid is 10 8 -10 9 cfu / mL.

[0047] Further, the preparation method of the silymarin extract includes the following steps:

[0048] S1. Wash and dry the silymarin fruits, remove oil by the pressing method, and make a pulp with 5 - 10 times water.

[0049] S2. Add a complex enzyme composed of cellulase and pectinase to the above pulp, control the temperature at 45 - 55 °C, the pH at 4.5 - 5.5, and the pressure at 120 - 350 mpa, and carry out enzymolysis to obtain an enzymolysis solution.

[0050] S3. Extract the above enzymolysis solution with absolute ethanol at room temperature for 1 - 3 times, combine the multiple extraction solutions, concentrate, remove the solvent, and carry out spray drying to obtain the silymarin extract.

[0051] As a preferred technical solution, the addition amount of the complex enzyme is 0.2 - 5.0 wt%.

[0052] As a preferred technical solution, the weight ratio of the cellulase to the pectinase is 2 - 5:1, and the enzymolysis time is 30 - 70 min.

[0053] In the second aspect, the present invention provides a preparation method of an oyster peptide powder composition, and the preparation method includes: weighing the oyster peptide powder, silymarin extract, ampelopsis grossedentata extract, and licorice extract according to the ratio, and mixing them evenly to obtain the composition.

[0054] In a third aspect, the present invention provides an application of an oyster peptide powder composition in the preparation of a liver-protecting product.

[0055] Further, the product is a medicine, and the product further includes pharmaceutically acceptable excipients.

[0056] Further, the pharmaceutically acceptable excipients include at least one of a carrier, an excipient, and a diluent.

[0057] Preparation Example 1

[0058] This preparation example provides a preparation method of oyster peptide powder, including the following steps:

[0059] S1, Take the washed oyster meat, crush it, add 15 times the volume of water and mix, add 3.5% animal protease, adjust the pH to 7.5, the temperature to 60 °C, carry out an enzymatic hydrolysis reaction for 5 h, inactivate the enzyme, and centrifuge or filter to obtain an enzymatic hydrolysis filtrate;

[0060] S2, Add water to the filter residue and homogenize it with a high-pressure homogenizer, sterilize it at 121 °C for 20 min to obtain a homogenate, and cool it for later use;

[0061] S3, Add the Lactobacillus reuteri seed liquid with a bacterial content of 10 8 cfu / mL and an inoculation amount of 2 v / v% to the above homogenate, ferment at 40 °C for 36 h, centrifuge to remove bacteria, collect the supernatant to obtain a fermentation broth;

[0062] S4, Mix the enzymatic hydrolysis filtrate and the fermentation broth, concentrate, and spray dry to obtain the oyster peptide powder.

[0063] The oyster peptide powder of this preparation example is used in the following examples and comparative examples.

[0064] Preparation Example 2

[0065] This preparation example provides a preparation method of silymarin extract, including the following steps:

[0066] S1, Wash and dry the milk thistle fruits, remove oil by the pressing method, and add 10 times water to make a pulp;

[0067] S2, Add a composite enzyme composed of cellulase and pectinase with a weight ratio of 4:1 to the above pulp, the addition amount of the composite enzyme is 4%, control the temperature at 50 °C, the pH at 5.0, and the pressure at 300 mpa, and carry out enzymatic hydrolysis for 40 min to obtain an enzymatic hydrolysate;

[0068] S3, Extract the above enzymatic hydrolysate with anhydrous ethanol at room temperature 3 times, combine the 3 extraction liquids, concentrate, remove the solvent, and carry out spray drying to obtain silymarin extract.

[0069] The silybum marianum extract of this preparation example is used in the following examples and comparative examples.

[0070] Example 1

[0071] An oyster peptide powder composition comprising the following components in parts by weight: 40 parts of oyster peptide powder, 15 parts of silybum marianum extract, 6 parts of ampelopsis grossedentata extract, and 6 parts of licorice extract.

[0072] Example 2

[0073] An oyster peptide powder composition comprising the following components in parts by weight: 23 parts of oyster peptide powder, 7 parts of silybum marianum extract, 2 parts of ampelopsis grossedentata extract, and 2 parts of licorice extract.

[0074] Example 3

[0075] An oyster peptide powder composition comprising the following components in parts by weight: 30 parts of oyster peptide powder, 10 parts of silybum marianum extract, 4 parts of ampelopsis grossedentata extract, and 4 parts of licorice extract.

[0076] The preparation method of the oyster peptide powder composition of Examples 1 - 3 is: weigh the oyster peptide powder, silybum marianum extract, ampelopsis grossedentata extract, and licorice extract according to the ratio, and mix them evenly to obtain.

[0077] Comparative Example 1

[0078] The difference between this comparative example and Example 3 is that it does not contain oyster peptide powder, and the others are the same.

[0079] The preparation method is the same as that of Example 3.

[0080] Comparative Example 2

[0081] The difference between this comparative example and Example 3 is that it does not contain silybum marianum extract, and the others are the same.

[0082] The preparation method is the same as that of Example 3.

[0083] Comparative Example 3

[0084] The difference between this comparative example and Example 3 is that it does not contain ampelopsis grossedentata extract, and the others are the same.

[0085] The preparation method is the same as that of Example 3.

[0086] Comparative Example 4

[0087] The difference between this comparative example and Example 3 is that it does not contain licorice extract, and the others are the same.

[0088] The preparation method is the same as that of Example 3.

[0089] Comparative Example 5

[0090] This comparative example is different from Example 3 in that: the oyster peptide powder is replaced with commercially available oyster peptide (food grade, purchased from Hebei Runbu Biotechnology Co., Ltd.), and the others are the same.

[0091] The preparation method is the same as that of Example 3.

[0092] Comparative Example 6

[0093] This comparative example is different from Example 3 in that: the preparation method of the oyster peptide powder does not include steps S1 and S3, and the enzymolysis solution obtained in step S1 is directly concentrated and spray-dried to obtain the oyster peptide powder, and the others are the same.

[0094] The preparation method is the same as that of Example 3.

[0095] Comparative Example 7

[0096] This comparative example is different from Example 3 in that: the silymarin extract is replaced with commercially available silymarin extract (food grade, purchased from Shaanxi Junhe Biotechnology Co., Ltd.), and the others are the same.

[0097] The preparation method is the same as that of Example 3.

[0098] Comparative Example 8

[0099] This comparative example is different from Example 3 in that: only cellulase is used in step S2 of the preparation method of the silymarin extract, and the others are the same.

[0100] The preparation method is the same as that of Example 3.

[0101] Experimental Example 1 Verification of Animal Experiment for Alleviating Alcoholic Liver Injury

[0102] 1. Animal Modeling and Administration

[0103] Take Kunming mice, male, weighing 18 - 22 g. After 1 week of adaptive feeding, the mice are randomly divided into a normal group, a model group, a positive group (bifendate 150 mg / kg), and groups of Examples 1 - 3 and Comparative Examples 1 - 8 (0.5 g / kg of the corresponding prepared oyster peptide powder composition), with 10 mice in each group. The normal group and the model group are given equal volumes of distilled water by gavage. Each administration group is given the drug according to the corresponding dose. 30 minutes later, except for the normal group which is given distilled water by gavage, the other groups are given 56% white wine by gavage at a dose of 11.0 mL / kg once a day for 7 consecutive days. If it is observed during the experiment that the hair of the mice in the model group turns yellow, listless, has a decreased appetite, and a significant weight loss, it indicates that the modeling is successful. Fast the mice for 24 hours without water restriction, take blood from the eye socket, let it stand for 2 hours, then centrifuge, and take the upper serum and store it in a -80°C low-temperature refrigerator.

[0104] 2. Detection Indexes

[0105] The content of mouse tumor necrosis factor-α (TNF-α) in liver tissues was detected using an ELISA kit. 200 μL of serum was taken to measure the activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in the serum with an automatic biochemical analyzer. The activity of total superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in liver tissues were measured according to the method described in the kit instructions.

[0106] 3. Experimental results

[0107] The activities of ALT and AST in mouse serum and the contents of TNF-α, SOD, and MDA in liver tissues were detected, and the test results are shown in Table 1.

[0108] Table 1 Effects of oyster peptide powder composition on alcoholic liver injury in mice (n = 10)

[0109]

[0110]

[0111]

[0112] * Compared with the normal group, P < 0.05, ** compared with the normal group, P < 0.01; # compared with the model group, P < 0.05, ## compared with the normal group, P < 0.01.

[0113] As can be seen from Table 1 above, by detecting the activities of ALT and AST in serum and the contents of TNF-α, SOD, and MDA in liver tissues, it was found that compared with the normal group, the contents of ALT, AST, TNF-α, and MDA in the model group were significantly increased (P < 0.05 or P < 0.01), and the content of SOD decreased (P < 0.05), showing significant differences. This indicates that the model was successfully established, and the liver cells in the model group showed varying degrees of damage.

[0114] Compared with the model group, the contents of ALT, AST, TNF-α, and MDA in the positive group and Examples 1 - 3 were significantly decreased (P < 0.05 or P < 0.01), and the content of SOD increased (P < 0.05), showing significant differences. This indicates that the oyster peptide powder composition prepared in Examples 1 - 3 of the present invention can significantly reduce the content of TNF-α, decrease the activities of ALT and AST, increase the activity of SOD, and reduce the content of MDA, showing a good effect in alleviating alcoholic liver injury. Among them, Example 2 achieved relatively close results in improving liver cell damage compared with the positive group.

[0115] Compared with Example 3, Comparative Example 1 did not contain oyster peptide powder, and the contents of ALT, AST, TNF-α, and MDA all increased, while the content of SOD decreased. This shows that adding oyster peptide powder can enhance the improvement effect on liver cell damage.

[0116] Compared with Example 3, Comparative Example 2 does not contain silymarin extract, and the contents of ALT, AST, TNF-α and MDA all increase, while the content of SOD decreases. This shows that adding silymarin extract can enhance the improvement effect on liver cell damage.

[0117] Compared with Example 3, Comparative Example 3 does not contain Ampelopsis grossedentata extract. The contents of ALT, AST, TNF-α and MDA all increase, while the content of SOD decreases. This shows that adding Ampelopsis grossedentata extract can enhance the improvement effect on liver cell damage.

[0118] Compared with Example 3, Comparative Example 4 does not contain licorice extract, and the contents of ALT, AST, TNF-α and MDA all increase, while the content of SOD decreases. This shows that adding licorice extract can enhance the improvement effect on liver cell damage.

[0119] From the test results of Comparative Examples 1-4, it can be seen that oyster peptide powder, silymarin extract, Ampelopsis grossedentata extract and licorice extract can act synergistically to enhance the effect of improving liver cell damage.

[0120] Compared with Example 3, in Comparative Example 5, the oyster peptide powder is replaced with commercially available oyster peptide. The contents of ALT, AST, TNF-α and MDA increase somewhat, and the content of SOD decreases somewhat. This shows that the oyster peptide powder obtained by the preparation method of oyster peptide powder provided by the present invention has a better effect on improving liver cell damage. The reason may be that the oyster peptide powder prepared by the present invention contains the fermentation product of Lactobacillus reuteri and the enzymatic hydrolysate of animal protease, and the nutritional components are more abundant.

[0121] Compared with Example 3, in Comparative Example 6, the preparation method of oyster peptide powder does not include steps S1 and S3. The enzymatic hydrolysate obtained in step S1 is directly concentrated and spray-dried to obtain oyster peptide powder. The contents of ALT, AST, TNF-α and MDA increase somewhat, and the content of SOD decreases somewhat.

[0122] Compared with Example 3, in Comparative Example 7, the silymarin extract is replaced with commercially available silymarin extract. The contents of ALT, AST, TNF-α and MDA increase somewhat, and the content of SOD decreases somewhat. This shows that the silymarin extract obtained by the preparation method of silymarin extract provided by the present invention has a better effect on improving liver cell damage.

[0123] Compared with Example 3, in Comparative Example 8, only cellulase is used in step S2 of the preparation method of silymarin extract. The contents of ALT, AST, TNF-α and MDA increase somewhat, and the content of SOD decreases somewhat.

[0124] In summary, the oyster peptide powder composition provided by the present invention has a significant effect on improving liver injury through the synergistic action of four components.

[0125] Although the embodiments of the present description have been shown and described, it will be understood by those of ordinary skill in the art that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principles and spirit of the present description, and the scope of the present description is defined by the appended claims and their equivalents.

Claims

1. An oyster peptide powder composition, characterized in that: The invention comprises the following components in parts by weight: 20-40 parts of oyster peptide powder, 8-15 parts of milk thistle extract, 2-6 parts of rattan tea extract and 2-6 parts of liquorice extract; The preparation method of the oyster peptide powder comprises the following steps: S1, taking cleaned oyster meat, blending it, mixing it with water, adding protease, adjusting pH, performing enzymolysis reaction, inactivating enzyme, centrifuging or filtering, and obtaining enzymolysis filtrate; S2, adding water to the filter residue and homogenizing it with a high-pressure homogenizer, sterilizing it at 121-135° C. for 15-25 minutes, obtaining a homogenate, and cooling it for standby use; S3, adding lactic acid bacteria seed liquid to the homogenate, fermenting it at 30-45° C. for 6-72 hours, and obtaining a fermentation liquid; S4, mixing the enzymolysis filtrate and the fermentation liquid, concentrating them, and spray-drying them to obtain the oyster peptide powder; the protease is animal protease; the lactic acid bacteria is Lactobacillus reuteri; the inoculation amount of the lactic acid bacteria seed liquid is 1-5v / v%, and the bacterial content of the seed liquid is 10 8 -10 9 cfu / mL; the preparation method of the milk thistle extract comprises the following steps: S1, cleaning and drying the milk thistle fruit, removing oil by squeezing, and pulping with 5-10 times of water; S2, adding a complex enzyme composed of cellulase and pectinase to the pulp, controlling the temperature to 45-55°C, the pH to 4.5-5.5, and the pressure to 120-350mpa, performing enzymolysis to obtain an enzymolysis solution; S3, extracting the enzymolysis solution with anhydrous ethanol at room temperature for 1-3 times, combining the multiple extracts, concentrating, removing the solvent, and spray drying to obtain the milk thistle extract; the weight ratio of the cellulase to the pectinase is 2-5:1, and the enzymolysis time is 30-70min.

2. The method for preparing the oyster peptide powder composition according to claim 1, characterized in that: The preparation method comprises: weighing oyster peptide powder, milk thistle extract, rattan tea extract and licorice extract according to a proportion, and mixing them evenly to obtain the product.

3. The use of the oyster peptide powder composition according to claim 1 in preparing a product for alleviating alcoholic liver damage, characterized in that: The product is a medicine, and the product also includes pharmaceutically acceptable excipients.

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