A method and system for intervention in enriching the environment of mice

Abstract

The present application discloses an intervention method and system for enriching the environment of mice, the method comprising selecting an experimental mouse sample library from a large number of mice, dividing the mice in the experimental mouse sample library into five equal parts to obtain the mouse sample grouping results; based on the mouse sample grouping results, detecting the oxytocin concentration in the control mice to obtain the control oxytocin concentration data; based on the mouse sample grouping results, changing the visual environment of the mice and analyzing the impact on the oxytocin concentration of the mice to output the visual environment impact results; based on the mouse sample grouping results, changing the auditory environment of the mice and analyzing the impact on the oxytocin concentration of the mice to output the auditory environment impact results; based on the mouse sample grouping results, changing the odor environment of the mice and analyzing the impact on the oxytocin concentration of the mice to output the olfactory environment impact results. The present application has the effect of improving the accuracy of the detection of the social function of mice regulated by the environment.

Description

Technical Field

[0001] The present application relates to the field of data processing technology, and in particular to an intervention method and system for enriching the environment of mice. Background Art

[0002] At present, among mammalian experimental animals, mice play an important role in scientific research because of their small size, easy feeding and management, easy control, fast reproduction, the most in-depth research, and clear quality control standards. Mice are widely used in various laboratory research from basic biology to medical research. With the development of science and technology, the multi-sensory enriched environment mouse training system is becoming more and more perfect, so data processing of the multi-sensory enriched environment mouse training system is crucial.

[0003] The existing intervention method for enriching the environment of mice is to give mice a single stimulus and judge the social ability of mice by their behavioral performance or the completion of specific tasks. This may lead to certain subjectivity and limitations in the objective evaluation of the training effect of mice. The accuracy of detecting the impact of the environment on the social function of mice is low, and there is room for improvement. Summary of the invention

[0004] In order to improve the detection accuracy of environmental regulation of the social function of mice, the present application provides an intervention method and system for enriching the environment of mice.

[0005] In the first aspect, the present application provides an intervention method for enriching the environment of mice, which adopts the following technical solution:

[0006] An intervention method for enriching the environment of mice, comprising the following steps:

[0007] An experimental mouse sample library is selected from a large number of mice, and the mice in the experimental mouse sample library are divided into five equal parts to obtain the mouse sample grouping results;

[0008] Based on the results of mouse sample grouping, the oxytocin concentration in the control mice was detected to obtain the control oxytocin concentration data;

[0009] Based on the results of mouse sample grouping, the visual environment of the mice was changed and the effect on the oxytocin concentration of the mice was analyzed to output the results of the visual environment impact;

[0010] Based on the results of mouse sample grouping, the auditory environment of the mice was changed and the effect on the oxytocin concentration of the mice was analyzed to output the results of the auditory environment impact;

[0011] Based on the results of mouse sample grouping, the odor environment in which the mice are located is changed and the effect on the oxytocin concentration of the mice is analyzed to output the results of the olfactory environment impact;

[0012] Based on the results of mouse sample grouping, the touch environment of the mice was changed and the effect on the oxytocin concentration of the mice was analyzed to output the results of the tactile environment impact;

[0013] The environmental information of the mouse to be tested is obtained, the physical condition of the mouse to be tested is detected to obtain the physical condition information of the mouse, and the concentration of oxytocin produced by the mouse is controlled based on the environmental information of the mouse to be tested, the physical condition information of the mouse, the visual environment impact results, the auditory environment impact results, the olfactory environment impact results and the tactile environment impact results.

[0014] Preferably, a large number of mice are obtained to obtain overall information of the mice, and the ages of all the mice are obtained based on the overall information of the mice to obtain the age information of the mice;

[0015] Comparing the mouse age information with the preset mouse standard age range to obtain the mouse age comparison result;

[0016] Based on the mouse age comparison result, mice whose age information exceeds a preset mouse standard age range are screened and removed, and mice whose age information is within the preset mouse standard age range are retained to obtain a first mouse screening result;

[0017] Weighing the mice based on the first mouse screening result to obtain mouse weight information, and comparing the mouse weight information with a preset mouse standard weight range to obtain a mouse weight comparison result;

[0018] Based on the mouse weight comparison result, mice whose weight information exceeds the preset mouse standard weight range are screened and removed, and mice whose weight information is within the preset mouse standard weight range are retained to obtain a second mouse screening result;

[0019] Based on the second mouse screening result, the mouse medical history information and the mouse family medical history information are obtained, mice with sequelae diseases in the mouse medical history information and mice with genetic diseases in the mouse family medical history information are screened and removed, and the screened and removed mice are retained to obtain an experimental mouse sample library;

[0020] Based on the experimental mouse sample library, the mice were divided into five equal parts to obtain the mouse sample grouping results, which included five sub-sample libraries, namely, a control sub-sample library, a first sub-sample library, a second sub-sample library, a third sub-sample library and a fourth sub-sample library.

[0021] Preferably, a fluorescent OT sensor is obtained and a signal connection link is established between the sensitive fluorescent OT sensor and a control subsample library;

[0022] The mice in the control sub-sample library were placed in an environment with suitable temperature and humidity, and the oxytocin concentration in the mice in the control sub-sample library was detected in real time based on the sensitive fluorescent OT sensor to obtain the control oxytocin concentration data.

[0023] Preferably, the mice in the first sub-sample library in the mouse sample grouping result are placed in a first mouse cage, wherein the inner wall of the first mouse cage is provided with an LED display screen;

[0024] The color hue on the LED display screen is changed in sequence, the color hue retention time of each hue on the LED display screen is a fixed value, the time interval between the color hues of every two hues is a preset color retention time, and the mice are rewarded by supplementing food and water during the color retention time;

[0025] Based on the sensitive fluorescent OT sensor, the concentration of oxytocin in each hue during the residence time of the color hue is detected to obtain multiple hue oxytocin concentration data;

[0026] Compare the oxytocin concentrations of multiple hues with the control oxytocin concentration data and calculate the difference to obtain the oxytocin concentration change data of each hue, and compare and sort the oxytocin concentration change data of each hue to obtain the hue influence result;

[0027] The hue represented by the middle value of the hue influence result is recorded as the designated hue, the color brightness of the designated hue is changed from light to dark on the LED display screen, the body temperature of the mice in the first sub-sample library is detected to obtain the first mouse body temperature information, the first mouse body temperature information is compared with the preset mouse standard body temperature information and the absolute value is taken to obtain the first mouse body temperature difference;

[0028] Based on the temperature difference of the first mouse, the color brightness change amplitude of the specified hue and the color brightness retention time of each color brightness on the LED display screen are determined, and the color brightness retention time interval between every two brightnesses is the preset color retention time, and the mouse is rewarded by supplementing food and water during the color retention time;

[0029] Based on the sensitive fluorescent OT sensor, the oxytocin concentration during the brightness residence time of each brightness color is detected to obtain multiple brightness oxytocin concentration data;

[0030] Compare multiple brightness oxytocin concentration data with control oxytocin concentration data and calculate the difference to obtain oxytocin concentration change data of each brightness, and compare and sort the oxytocin concentration change data of each brightness to obtain brightness influence results;

[0031] The hue influence result and the brightness influence result are combined to form a visual environment influence result.

[0032] Preferably, the mice in the second sub-sample library in the mouse sample grouping result are placed in a second mouse cage, a speaker is provided on the inner wall of the second mouse cage, different sound types are played based on the speaker, the type playing time of each sound type is a fixed value, the time interval between every two types of playing is a preset sound retention time, and the mice are rewarded by supplementing food and water during the sound retention time;

[0033] Based on the sensitive fluorescent OT sensor, the oxytocin concentration of the sound type during the sound residence time is detected to obtain multiple sound type oxytocin concentration data, the multiple sound type oxytocin concentration data are compared with the control oxytocin concentration data, and the difference is calculated to obtain the oxytocin change concentration data of each sound type, and the oxytocin change concentration data of each sound type are compared and sorted to obtain the sound type impact result;

[0034] The sound type represented by the middle value in the sound type influence result is recorded as the designated sound type, the frequency of the designated sound type is played in sequence from low to high based on the speaker, the body temperature of the mice in the second subsample library is detected to obtain the second mouse body temperature information, the second mouse body temperature information is compared with the preset mouse standard body temperature information and the absolute value is taken to obtain the second mouse body temperature difference;

[0035] Based on the body temperature difference of the second mouse, the frequency change amplitude of the sound type played by the speaker and the sound frequency retention time of each sound frequency are determined, the time interval between the sound frequency retention of every two frequencies is the preset sound retention time, and the mouse is rewarded by supplementing food and water during the sound retention time;

[0036] Based on the sensitive fluorescent OT sensor, the oxytocin concentration within the sound frequency residence time of different sound frequencies is detected to obtain the oxytocin concentration data of multiple frequencies, the oxytocin concentration data of multiple frequencies are compared with the control oxytocin concentration data and the difference is calculated to obtain the oxytocin concentration change data of each sound frequency, and the oxytocin concentration change data of each sound frequency are compared and sorted to obtain the sound frequency influence result;

[0037] The sound frequency represented by the middle value in the sound frequency impact result is the designated sound frequency, and the designated sound type and the designated sound frequency are played in order from low to high based on the speaker, and the sound with different sound intensities is played based on the speaker, and the intensity playing time of each sound intensity is a fixed value, and the time interval between the playing of each two intensities is a preset sound retention time, and the mice are rewarded with food and water during the sound retention time;

[0038] Based on the sensitive fluorescent OT sensor, the oxytocin concentration of different sound intensities during the playing time is detected to obtain multiple sound intensity oxytocin concentration data, the multiple sound intensity oxytocin concentration data are compared with the control oxytocin concentration data and the difference is calculated to obtain the oxytocin change concentration data of each sound intensity, and the oxytocin change concentration data of each sound intensity are compared and sorted to obtain the sound intensity impact result;

[0039] The sound type influence result, the sound frequency influence result and the sound intensity influence result are combined to form an auditory environment influence result.

[0040] Preferably, the mice in the third sub-sample library in the mouse sample grouping result are placed in a third mouse cage, and the third mouse cage is subjected to an air purification operation;

[0041] Inject air with different odor properties into the third mouse cage, the odor property residence time of the air with different odor properties in the third mouse cage is a fixed value, the time interval between the residence of every two odor properties is a preset odor residence time, and during the odor residence time, food and water are added to reward the mice and the air of the third mouse cage is purified;

[0042] Based on the sensitive fluorescent OT sensor, the mouse oxytocin concentration when the air with different odor properties was injected into the third mouse cage was detected to obtain multiple odor property oxytocin concentration data;

[0043] Compare the oxytocin concentration data of multiple odor properties with the control oxytocin concentration data and calculate the difference to obtain the oxytocin concentration change data of each odor property, and compare and sort the oxytocin concentration change data of each odor property to obtain the influence result of the odor property;

[0044] The odor property represented by the middle value of the odor property influence result is recorded as the designated odor property, the odor concentration of the designated odor property is injected into the third mouse cage, wherein the odor concentration of the designated odor property is from low to high, the body temperature of the mice in the third sub-sample library is detected to obtain the body temperature information of the third mouse, the body temperature information of the third mouse is compared with the preset standard body temperature information of the mouse, and the absolute value is taken to obtain the body temperature difference of the third mouse;

[0045] Determine the change range of the odor concentration of the specified odor property and the odor concentration residence time of each odor concentration based on the body temperature difference of the third mouse, the odor concentration residence time interval between every two odor concentrations is the preset odor residence time, and reward the mouse with food and water during the odor residence time and perform air purification operation on the third mouse cage;

[0046] The oxytocin concentration of mice during the odor concentration residence time of each odor concentration was detected based on the sensitive fluorescent OT sensor to obtain multiple odor concentration oxytocin concentration data;

[0047] Compare the oxytocin concentration data of multiple odor concentrations with the control oxytocin concentration data and calculate the difference to obtain the oxytocin concentration change data of each odor concentration, and compare and sort the oxytocin concentration change data of each odor concentration to obtain the odor concentration influence result;

[0048] The odor property influence result and the odor concentration influence result are combined to form the olfactory environment influence result.

[0049] Preferably, the mice in the fourth sub-sample library in the mouse sample grouping result are placed in a fourth mouse cage, a replacement layer is provided at the bottom of the fourth mouse cage, and a temperature control layer is provided below the replacement layer; wherein the replacement layer is provided with a replaceable mouse touch object, and the temperature control layer includes a temperature control plate, and the temperature control plate is used to control the temperature of the mouse touch object;

[0050] Place touch objects with different smoothness in the replacement layer, the touch object residence time of the touch objects with different smoothness is a fixed value, the touch object residence time of every two touch objects with different smoothness is a preset touch residence time, and the mice are rewarded with food and water during the touch residence time, and the touch object most frequently used by the mice is placed in the replacement layer;

[0051] Based on the sensitive fluorescent OT sensor, the oxytocin concentration data of mice with touch objects of different smoothness placed on the replacement layer in the fourth mouse cage were detected to obtain multiple touch object oxytocin concentration data;

[0052] Compare the oxytocin concentration data of multiple touch objects with the oxytocin concentration data of the control and calculate the difference to obtain the oxytocin concentration change data of touch objects with different smoothness, and compare and sort the oxytocin concentration change data of touch objects with different smoothness to obtain the touch object impact result;

[0053] The touch object represented by the middle value of the touch object influence result is recorded as the designated touch object, the designated touch object is placed in the replacement layer, the temperature of the designated touch object is changed based on the temperature control board, the body temperature of the mice in the fourth sub-sample library is detected to obtain the fourth mouse body temperature information, the fourth mouse body temperature information is compared with the preset mouse standard body temperature information and the absolute value is taken to obtain the fourth mouse body temperature difference value;

[0054] Determine the temperature change amplitude of the temperature control plate based on the fourth mouse body temperature difference, set the temperature change time interval of the temperature control plate to a preset touch residence time, replenish food and water to reward the mouse during the touch residence time, place the most frequently touched object of the mouse in the replacement layer and adjust the temperature control plate to a suitable temperature for the mouse;

[0055] Based on the sensitive fluorescent OT sensor, the mouse oxytocin concentration when the designated touch object is at different temperatures is detected to obtain multiple temperature oxytocin concentration data;

[0056] Compare the oxytocin concentration data at multiple temperatures with the control oxytocin concentration data and calculate the difference to obtain the oxytocin concentration change data at different temperatures, and compare and sort the oxytocin concentration change data at different temperatures to obtain the temperature influence result of the touch object;

[0057] The touch object influence result and the touch object temperature influence result are combined to form a tactile environment influence result.

[0058] Preferably, the environment information of the mouse to be tested is obtained, and the complexity of the mouse environment layout is obtained based on the environment information of the mouse to be tested, wherein the complexity of the mouse environment layout includes the complexity of the mouse visual environment layout, the complexity of the mouse auditory environment layout, the complexity of the mouse olfactory environment layout and the complexity of the mouse tactile environment layout;

[0059] Detecting the physical condition of the mouse to be tested to obtain the physical condition information of the mouse, and judging the sensory perception degree of the mouse based on the physical condition information of the mouse, wherein the sensory perception degree of the mouse includes the visual perception degree of the mouse, the auditory perception degree of the mouse, the olfactory perception degree of the mouse, and the tactile perception degree of the mouse;

[0060] Determining the mouse perception adjustment items based on the complexity of the mouse environment layout and the mouse sensory perception degree, wherein the mouse perception adjustment items include vision, hearing, smell and touch;

[0061] The oxytocin concentration produced by mice is regulated based on the mouse's perception regulation items, visual environment influence results, auditory environment influence results, olfactory environment influence results and tactile environment influence results.

[0062] In a second aspect, the present application provides an intervention system for enriching the environment of mice, which adopts the following technical solutions:

[0063] An intervention system for an enriched environment in mice, comprising:

[0064] An experimental mouse selection module is configured to select an experimental mouse sample library from a large number of mice, and divide the mice in the experimental mouse sample library into five equal parts to obtain a mouse sample grouping result;

[0065] A control group module is configured to detect the oxytocin concentration in the control mice based on the mouse sample grouping results to obtain control oxytocin concentration data;

[0066] A visual environment analysis module is configured to change the visual environment of the mice based on the mouse sample grouping results, analyze the impact on the oxytocin concentration of the mice, and output the visual environment impact results;

[0067] An auditory environment analysis module is configured to change the auditory environment of the mice based on the mouse sample grouping results, analyze the impact on the oxytocin concentration of the mice, and output the auditory environment impact results;

[0068] The olfactory environment analysis module is configured to change the odor environment of the mice based on the mouse sample grouping results, analyze the impact on the oxytocin concentration of the mice, and output the olfactory environment impact results;

[0069] A tactile environment analysis module is configured to change the touch environment of the mouse based on the mouse sample grouping results, analyze the impact on the mouse oxytocin concentration, and output the tactile environment impact results;

[0070] The oxytocin regulation module is configured to obtain the environmental information of the mouse to be tested, detect the physical condition of the mouse to be tested to obtain the mouse physical condition information, and control the oxytocin concentration produced by the mouse based on the environmental information of the mouse to be tested, the mouse physical condition information, the visual environment impact results, the auditory environment impact results, the olfactory environment impact results and the tactile environment impact results.

[0071] In summary, the present application includes at least one of the following beneficial technical effects:

[0072] 1. The experimental mouse sample library is selected from a large number of mice and divided equally to obtain the mouse sample grouping results. Based on the mouse sample grouping results, the visual environment, auditory environment, odor environment and touch environment of the mice are changed respectively, and the visual environment impact results, auditory environment impact results, olfactory environment impact results and tactile environment impact results are obtained respectively. According to the environmental information of the mice to be tested, the physical condition information of the mice, the visual environment impact results, the auditory environment impact results, the olfactory environment impact results and the tactile environment impact results, the oxytocin concentration produced by the mice to be tested is adjusted, thereby improving the detection accuracy of oxytocin in the mouse brain. Oxytocin promotes social function, thereby improving the detection accuracy of the visual environment regulating the social function of mice;

[0073] 2. The mice were screened for the first time based on their age, screened for the second time based on their weight, and screened again based on their medical history information and family medical history information to obtain an experimental mouse sample library. Mice in good physical condition were screened for testing, which improved the accuracy of testing the social function of mice regulated by the environment;

[0074] 3. Obtain the complexity of the mouse environment layout with the help of the test mouse environment information, judge the mouse sensory perception level based on the mouse physical condition information, determine the mouse perception regulation item based on the complexity of the mouse environment layout and the mouse sensory perception level, and adjust the oxytocin concentration produced by the test mouse based on the mouse perception regulation item, thereby adjusting the social function of the test mouse. BRIEF DESCRIPTION OF THE DRAWINGS

[0075] Figure 1 This is a schematic diagram of the process of the intervention method for enriching the environment of mice in this embodiment;

[0076] Figure 2 This is a schematic diagram of the modules of the intervention system for enriching the environment of mice, which is mainly embodied in this embodiment.

[0077] Figure numerals: 1. Experimental mouse selection module; 2. Control group module; 3. Visual environment analysis module; 4. Auditory environment analysis module; 5. Olfactory environment analysis module; 6. Tactile environment analysis module; 7. Oxytocin regulation module. DETAILED DESCRIPTION

[0078] The present application is further described in detail below in conjunction with the accompanying drawings.

[0079] The present application embodiment discloses an intervention method for enriching the environment of mice.

[0080] An intervention method for enriching the environment of mice, comprising the following steps:

[0081] Reference Figure 1 Step S1, selecting an experimental mouse sample library from a large number of mice, and dividing the mice in the experimental mouse sample library into five equal parts to obtain the mouse sample grouping results. Step S1 specifically includes the following sub-steps:

[0082] Step S11, obtaining a large number of mice to obtain overall information of mice, wherein all mice in the overall information of mice are mice of the same breed, and obtaining the ages of all mice based on the overall information of mice to obtain mouse age information.

[0083] Step S12, comparing the mouse age information with a preset mouse standard age range to obtain a mouse age comparison result.

[0084] Step S13, based on the mouse age comparison result, the mice whose age information exceeds the preset mouse standard age range are screened and removed, and the mice whose age information is within the preset mouse standard age range are retained to obtain the first mouse screening result.

[0085] Step S14, weighing the mice based on the first mouse screening result to obtain mouse weight information, and comparing the mouse weight information with a preset mouse standard weight range to obtain a mouse weight comparison result.

[0086] Step S15, based on the mouse weight comparison result, the mice whose weight information exceeds the preset mouse standard weight range are screened and removed, and the mice whose weight information is within the preset mouse standard weight range are retained to obtain a second mouse screening result.

[0087] Step S16, based on the second mouse screening result, obtain the mouse medical history information and the mouse family medical history information, screen out the mice with sequelae diseases in the mouse medical history information and the mice with genetic diseases in the mouse family medical history information, and retain the mice after screening out to obtain an experimental mouse sample library.

[0088] Step S17, based on the experimental mouse sample library, the mice are divided into five equal parts to obtain the mouse sample grouping results, and the mouse sample grouping results include five sub-sample libraries, namely the control sub-sample library, the first sub-sample library, the second sub-sample library, the third sub-sample library and the fourth sub-sample library. The mice in each sub-sample library are placed in an environment with suitable temperature and humidity, and are provided with sufficient food and water.

[0089] In specific applications, when mice are older, their physical perception ability declines, and when they are younger, their sensory abilities are not fully developed, which affects the experimental results. Therefore, the mice are screened for the first time based on their age. When the mice are underweight or overweight, it may affect their perception ability. Therefore, the mice are screened for the second time based on their weight. The mice are then screened again based on their medical history information and their family medical history information to obtain a sample library of experimental mice. Mice in better physical condition are selected for testing, which improves the accuracy of detecting the social functions of environmentally regulated mice.

[0090] Reference Figure 1 , step S2, based on the mouse sample grouping results, detecting the oxytocin concentration in the control mouse to obtain the control oxytocin concentration data. Step S2 specifically includes the following sub-steps:

[0091] Step S21, obtaining a fluorescent OT sensor and establishing a signal connection link between the sensitive fluorescent OT sensor and a control sub-sample library.

[0092] Step S22, placing the mice in the control sub-sample library in an environment with suitable temperature and humidity, and detecting the oxytocin concentration in the mice in the control sub-sample library in real time based on the sensitive fluorescent OT sensor to obtain control oxytocin concentration data.

[0093] In specific applications, oxytocin promotes social function, and the sensitive fluorescent OT sensor can detect oxytocin in the mouse brain. In the embodiment of the present application, the oxytocin produced in the mouse brain in the control sub-sample library is detected by a sensitive fluorescent OT sensor, which is convenient for comparison with the oxytocin produced in the mouse brain in other sub-sample libraries, thereby improving the detection accuracy of the environment regulating the social function of mice.

[0094] Reference Figure 1Step S3, based on the mouse sample grouping results, the visual environment of the mouse is changed and the impact on the mouse oxytocin concentration is analyzed to output the visual environment impact results. Step S3 specifically includes the following sub-steps:

[0095] Step S31, putting mice in the first sub-sample library in the mouse sample grouping result into a first mouse cage, the inner wall of which is provided with an LED display screen.

[0096] Step S32, the color hue on the LED display screen is changed in sequence, the color hue residence time of each hue on the LED display screen is a fixed value, the color hue residence time interval between every two hues is a preset color residence time, and the mice are rewarded by supplementing food and water during the color residence time.

[0097] Step S33, detecting the oxytocin concentration of each hue during the color hue residence time based on the sensitive fluorescent OT sensor to obtain a plurality of hue oxytocin concentration data.

[0098] Step S34, comparing the oxytocin concentrations of multiple hues with the control oxytocin concentration data and calculating the difference to obtain the oxytocin concentration change data of each hue, and comparing and sorting the oxytocin concentration change data of each hue to obtain the hue influence result.

[0099] Step S35, record the hue represented by the middle value of the hue influence result as the designated hue, change the color brightness of the designated hue from light to dark on the LED display screen, detect the body temperature of the mice in the first sub-sample library to obtain the first mouse body temperature information, compare the first mouse body temperature information with the preset mouse standard body temperature information and take the absolute value to obtain the first mouse body temperature difference.

[0100] Step S36, based on the first mouse body temperature difference, determine the color brightness change amplitude of the specified hue on the LED display screen and the color brightness residence time of each color brightness, the color brightness residence time interval of each two brightnesses is the preset color residence time, and the mice are rewarded by supplementing food and water during the color residence time. When the mouse body temperature is not at the standard body temperature threshold, the mouse comfort is low, the mouse concentration ability is poor, and the mouse needs a longer reaction time to the change of the external environment, so the larger the first mouse body temperature difference, the smaller the color brightness change amplitude and the longer the color brightness residence time, and the smaller the first mouse body temperature difference, the larger the color brightness change amplitude and the shorter the color brightness residence time.

[0101] Step S37, detecting the oxytocin concentration within the brightness residence time of each brightness color based on the sensitive fluorescent OT sensor to obtain a plurality of brightness oxytocin concentration data.

[0102] Step S38, compare the plurality of brightness oxytocin concentration data with the control oxytocin concentration data and calculate the difference to obtain the oxytocin concentration change data of each brightness, and compare and sort the oxytocin concentration change data of each brightness to obtain the brightness impact result.

[0103] Step S39, the hue influence result and the brightness influence result are combined to form a visual environment influence result.

[0104] In specific application, multiple hue oxytocin concentration data are obtained by changing the hue of the inner wall of the first mouse cage and detecting the oxytocin concentration of mice, and the hue influence results are obtained based on the hue oxytocin concentration data. Multiple brightness oxytocin concentration data are obtained by changing the brightness of the inner wall of the first mouse cage and detecting the oxytocin concentration of mice. The brightness influence results are obtained based on the brightness oxytocin concentration data. The visual environment influence results are obtained by combining the hue influence results and the brightness influence results. This improves the detection accuracy of the visual environment regulating oxytocin in the mouse brain. Oxytocin promotes social function, thereby improving the detection accuracy of the visual environment regulating the social function of mice.

[0105] Reference Figure 1 Step S4, based on the mouse sample grouping results, the auditory environment of the mice is changed and the impact on the oxytocin concentration of the mice is analyzed to output the auditory environment impact results. Step S4 specifically includes the following sub-steps:

[0106] Step S41, putting the mice in the second sub-sample library in the mouse sample grouping result into a second mouse cage, a speaker is set on the inner wall of the second mouse cage, and different types of sounds are played based on the speaker. The type playing time of each sound type is a fixed value, and the time interval between every two types of playback is a preset sound residence time. During the sound residence time, food and water are supplemented to reward the mice.

[0107] Step S42, based on the sensitive fluorescent OT sensor detecting the oxytocin concentration during the sound residence time of the sound type, obtaining multiple sound type oxytocin concentration data, comparing the multiple sound type oxytocin concentration data with the control oxytocin concentration data respectively and calculating the difference to obtain the oxytocin change concentration data of each sound type, and comparing and sorting the oxytocin change concentration data of each sound type to obtain the sound type impact result.

[0108] Step S43, record the sound type represented by the middle value in the sound type impact result as the specified sound type, play the specified sound type in sequence from low to high based on the speaker, detect the mouse body temperature in the second sub-sample library to obtain the second mouse body temperature information, compare the second mouse body temperature information with the preset mouse standard body temperature information and take the absolute value to obtain the second mouse body temperature difference.

[0109] Step S44, based on the second mouse body temperature difference, the sound frequency change amplitude of the speaker playing the specified sound type and the sound frequency residence time of each sound frequency are determined, and the sound frequency residence time interval of each two frequencies is the preset sound residence time, and the mouse is rewarded with food and water during the sound residence time. When the mouse body temperature is not at the standard body temperature threshold, the mouse comfort is low, the mouse concentration ability is poor, and the mouse needs a longer reaction time to the change of the external environment, so the larger the second mouse body temperature difference, the smaller the sound frequency change amplitude and the longer the sound frequency residence time, and the smaller the second mouse body temperature difference, the larger the sound frequency change amplitude and the shorter the sound frequency residence time.

[0110] Step S45, detecting the oxytocin concentration within the sound frequency residence time of different sound frequencies based on the sensitive fluorescent OT sensor to obtain multiple frequency oxytocin concentration data, comparing the multiple frequency oxytocin concentration data with the control oxytocin concentration data and calculating the difference to obtain the oxytocin change concentration data of each sound frequency, and comparing and sorting the oxytocin change concentration data of each sound frequency to obtain the sound frequency influence result.

[0111] Step S46, the sound frequency represented by the middle value in the sound frequency impact result is the designated sound frequency, and the designated sound type and the sound intensity of the designated sound frequency are played in sequence from low to high based on the speaker. The speaker plays sounds of different sound intensities, and the intensity playing time of each sound intensity is a fixed value. The time interval between each two intensities is the preset sound residence time. During the sound residence time, the mice are rewarded with food and water.

[0112] Step S47, detecting the oxytocin concentration of different sound intensities during the playing time based on the sensitive fluorescent OT sensor to obtain multiple sound intensity oxytocin concentration data, comparing the multiple sound intensity oxytocin concentration data with the control oxytocin concentration data and calculating the difference to obtain the oxytocin change concentration data of each sound intensity, and comparing and sorting the oxytocin change concentration data of each sound intensity to obtain the sound intensity impact result.

[0113] Step S48, the sound type influence result, the sound frequency influence result and the sound intensity influence result are combined to form the auditory environment influence result.

[0114] In specific applications, by playing sounds of different sound types to the mice in the second cage and detecting the oxytocin concentration of the mice, multiple sound type oxytocin concentration data are obtained, and the sound type influence results are obtained based on the sound type oxytocin concentration data. By playing sounds of different sound frequencies to the mice in the second cage and detecting the oxytocin concentration of the mice, multiple frequency oxytocin concentration data are obtained, and the sound frequency influence results are obtained based on the frequency oxytocin concentration data. By playing sounds of different sound intensities to the mice in the second cage and detecting the oxytocin concentration of the mice, multiple sound intensity oxytocin concentration data are obtained, and the sound intensity influence results are obtained based on the sound intensity oxytocin concentration data. The auditory environment influence results are obtained by combining the sound type influence results, the sound frequency influence results, and the sound intensity influence results, thereby improving the detection accuracy of the auditory environment regulating oxytocin in the mouse brain, and thereby improving the detection accuracy of the visual environment regulating the social function of the mouse.

[0115] Reference Figure 1 Step S5, based on the mouse sample grouping results, the odor environment of the mice is changed and the impact on the oxytocin concentration of the mice is analyzed to output the olfactory environment impact results. Step S5 specifically includes the following sub-steps:

[0116] Step S51, putting mice in the third sub-sample library in the mouse sample grouping result into a third mouse cage, and performing air purification operation on the third mouse cage.

[0117] Step S52, injecting air with different odor properties into the third cage, the odor property residence time of the air with different odor properties in the third cage is a fixed value, the time interval between every two odor properties is a preset odor residence time, and during the odor residence time, food and water are added to reward the mice and the air in the third cage is purified.

[0118] Step S53, based on the sensitive fluorescent OT sensor, the mouse oxytocin concentration when air with different odor properties is injected into the third mouse cage is detected to obtain multiple odor property oxytocin concentration data.

[0119] Step S54, comparing the oxytocin concentration data of multiple odor properties with the control oxytocin concentration data and calculating the difference to obtain the oxytocin concentration change data of each odor property, and comparing and sorting the oxytocin concentration change data of each odor property to obtain the odor property influence result.

[0120] Step S55, record the odor property represented by the middle value of the odor property influence result as the designated odor property, inject the odor concentration of the designated odor property into the third mouse cage, wherein the odor concentration of the designated odor property is from low to high, detect the body temperature of the mice in the third sub-sample library to obtain the third mouse body temperature information, compare the third mouse body temperature information with the preset mouse standard body temperature information and take the absolute value to obtain the third mouse body temperature difference.

[0121] Step S56, based on the third mouse body temperature difference, determine the change range of the odor concentration of the specified odor property and the odor concentration residence time of each odor concentration, the odor concentration residence time interval between every two odor concentrations is the preset odor residence time, and the mice are rewarded by replenishing food and water during the odor residence time, and the third mouse cage is air purified. When the mouse body temperature is not at the standard body temperature threshold, the mouse comfort is low, the mouse concentration ability is poor, and the mouse needs a longer reaction time to the changes in the external environment, so the greater the third mouse body temperature difference, the smaller the odor concentration change range and the longer the odor concentration residence time, and the smaller the third mouse body temperature difference, the greater the odor concentration change range and the shorter the odor concentration residence time.

[0122] Step S57, detecting the mouse oxytocin concentration within the odor concentration residence time of each odor concentration using the sensitive fluorescent OT sensor to obtain a plurality of odor concentration oxytocin concentration data.

[0123] Step S58, comparing the oxytocin concentration data of the multiple odor concentrations with the control oxytocin concentration data and calculating the difference to obtain the oxytocin concentration change data of each odor concentration, and comparing and sorting the oxytocin concentration change data of each odor concentration to obtain the odor concentration influence result.

[0124] Step S59, the odor property influence result and the odor concentration influence result are combined to form the olfactory environment influence result.

[0125] In specific application, by injecting air with different odor properties into mice in a third cage and detecting the oxytocin concentration of mice, multiple oxytocin concentration data of odor properties are obtained, and the odor property influence results are obtained based on the odor property oxytocin concentration data. By injecting different odor concentrations into mice in a third cage and detecting the oxytocin concentration of mice, multiple odor concentration oxytocin concentration data are obtained, and the odor concentration influence results are obtained based on the odor concentration oxytocin concentration data. The olfactory environment influence results are obtained by combining the odor property influence results and the odor concentration influence results, which improves the detection accuracy of the olfactory environment regulating oxytocin in the mouse brain, and thereby improves the detection accuracy of the olfactory environment regulating the social function of mice.

[0126] Reference Figure 1 , step S6, based on the mouse sample grouping results, the touch environment of the mouse is changed and the impact on the mouse oxytocin concentration is analyzed to output the tactile environment impact results. Step S6 specifically includes the following sub-steps:

[0127] Step S61, placing mice in the fourth sub-sample library in the mouse sample grouping result into a fourth mouse cage, wherein a replacement layer is provided at the bottom of the fourth mouse cage, and a temperature control layer is provided below the replacement layer. The replacement layer is provided with a replaceable mouse touch object, and the temperature control layer includes a temperature control board, which is used to control the temperature of the mouse touch object.

[0128] Step S62, placing touch objects with different smoothness in the replacement layer, the touch object residence time of the touch objects with different smoothness is a fixed value, the touch object residence time of every two touch objects with different smoothness is a preset touch residence time, and food and water are supplemented during the touch residence time to reward the mouse, and the touch object most frequently used by the mouse is placed in the replacement layer.

[0129] Step S63, based on the sensitive fluorescent OT sensor, the oxytocin concentration data of mice in the fourth mouse cage with touch objects of different smoothness placed on the replacement layer are detected to obtain a plurality of touch object oxytocin concentration data.

[0130] Step S64, comparing the oxytocin concentration data of the multiple touch objects with the control oxytocin concentration data and calculating the difference to obtain the oxytocin concentration change data of the touch objects with different smoothness, and comparing and sorting the oxytocin concentration change data of the touch objects with different smoothness to obtain the touch object impact result.

[0131] Step S65, record the touch object represented by the middle value of the touch object influence result as the designated touch object, place the designated touch object in the replacement layer, change the temperature of the designated touch object based on the temperature control board, detect the mouse body temperature in the fourth sub-sample library to obtain fourth mouse body temperature information, compare the fourth mouse body temperature information with the preset mouse standard body temperature information and take the absolute value to obtain the fourth mouse body temperature difference.

[0132] Step S66, based on the fourth mouse body temperature difference, the temperature change amplitude of the temperature control plate is determined, wherein the temperature change amplitude of the temperature control plate is negatively correlated with the fourth mouse body temperature difference. The temperature change time interval of the temperature control plate is a preset touch dwelling time, and the mouse is rewarded by replenishing food and water during the touch dwelling time, and the most frequently touched object of the mouse is placed in the replacement layer and the temperature control plate is adjusted to a suitable temperature for the mouse.

[0133] Step S67, obtaining a plurality of temperature oxytocin concentration data based on detecting the mouse oxytocin concentration when the designated touch object is at different temperatures using the sensitive fluorescent OT sensor.

[0134] Step S68, comparing the oxytocin concentration data at multiple temperatures with the control oxytocin concentration data and calculating the difference to obtain the oxytocin concentration change data at different temperatures, and comparing and sorting the oxytocin concentration change data at different temperatures to obtain the touch object temperature influence result.

[0135] Step S69: The touch object influence result and the touch object temperature influence result are combined to form a tactile environment influence result.

[0136] In specific application, by replacing touch objects with different degrees of smoothness in the fourth mouse cage and detecting the oxytocin concentration of mice, multiple touch object oxytocin concentration data are obtained, and the touch object influence results are obtained based on the touch object oxytocin concentration data. The temperature of the specified touch object is controlled by a temperature control panel and the oxytocin concentration of mice is detected to obtain multiple temperature oxytocin concentration data. The touch object temperature influence results are obtained based on the temperature oxytocin concentration data. The touch object influence results and the touch object temperature influence results are combined to obtain the tactile environment influence results, which improves the detection accuracy of the tactile environment regulating oxytocin in the mouse brain, thereby improving the detection accuracy of the tactile environment regulating the social function of the mouse.

[0137] Reference Figure 1 , step S7, obtaining the environment information of the mouse to be tested, detecting the physical condition of the mouse to be tested to obtain the physical condition information of the mouse, and controlling the concentration of oxytocin produced by the mouse based on the environment information of the mouse to be tested, the physical condition information of the mouse, the visual environment impact result, the auditory environment impact result, the olfactory environment impact result, and the tactile environment impact result. Step S7 specifically includes the following sub-steps:

[0138] Step S71, obtain the environment information of the mouse to be tested, and obtain the complexity of the mouse environment layout based on the environment information of the mouse to be tested. The complexity of the mouse environment layout includes the complexity of the mouse visual environment layout, the complexity of the mouse auditory environment layout, the complexity of the mouse olfactory environment layout and the complexity of the mouse tactile environment layout.

[0139] Step S72, detecting the physical condition of the mouse to be tested to obtain the mouse physical condition information, and judging the mouse sensory perception level based on the mouse physical condition information. The mouse sensory perception level includes the mouse visual perception level, the mouse auditory perception level, the mouse olfactory perception level and the mouse tactile perception level.

[0140] Step S73, based on the complexity of the mouse environment layout and the mouse sensory perception, the mouse perception adjustment item is determined, and the mouse perception adjustment item includes vision, hearing, smell and touch. For example, if the mouse to be tested has a hearing impairment, and the mouse visual environment layout has the lowest complexity, followed by the mouse olfactory environment layout complexity, and the mouse tactile environment layout has the highest complexity, then the mouse perception adjustment item is vision.

[0141] Step S74, adjusting the concentration of oxytocin produced by the mouse based on the mouse perception adjustment item, the visual environment impact result, the auditory environment impact result, the olfactory environment impact result, and the tactile environment impact result. For example, if the mouse perception adjustment item is vision, the mouse to be tested is visually stimulated based on the visual environment impact result, thereby increasing the concentration of oxytocin in the mouse brain, thereby improving the social function of the mouse to be tested.

[0142] In specific application, the complexity of the mouse environment layout is obtained through the environmental information of the mouse to be tested, the mouse sensory perception level is judged based on the mouse physical condition information, the mouse perception regulation items are determined based on the complexity of the mouse environment layout and the mouse sensory perception level, and the oxytocin concentration produced by the mouse to be tested is adjusted based on the mouse perception regulation items, thereby adjusting the social function of the mouse to be tested.

[0143] The embodiments of the present application also disclose an intervention system for enriching the environment of mice.

[0144] Reference Figure 2 , an intervention system for a mouse enriched environment includes: an experimental mouse selection module, configured to select an experimental mouse sample library from a large number of mice, and divide the mice in the experimental mouse sample library into five equal parts to obtain a mouse sample grouping result.

[0145] The control group module is configured to detect the oxytocin concentration in the control mice based on the mouse sample grouping results to obtain control oxytocin concentration data.

[0146] The visual environment analysis module is configured to change the visual environment of the mice based on the mouse sample grouping results, analyze the impact on the oxytocin concentration of the mice, and output the visual environment impact results.

[0147] The auditory environment analysis module is configured to change the auditory environment of the mice based on the mouse sample grouping results, analyze the impact on the oxytocin concentration of the mice, and output the auditory environment impact results.

[0148] The olfactory environment analysis module is configured to change the odor environment in which the mice are located based on the mouse sample grouping results, analyze the impact on the mouse oxytocin concentration, and output the olfactory environment impact results.

[0149] The tactile environment analysis module is configured to change the touch environment of the mouse based on the mouse sample grouping results, analyze the impact on the mouse oxytocin concentration, and output the tactile environment impact results.

[0150] The oxytocin regulation module is configured to obtain the environmental information of the mouse to be tested, detect the physical condition of the mouse to be tested to obtain the mouse physical condition information, and control the oxytocin concentration produced by the mouse based on the environmental information of the mouse to be tested, the mouse physical condition information, the visual environment impact results, the auditory environment impact results, the olfactory environment impact results and the tactile environment impact results.

[0151] The above are all preferred embodiments of the present application, and the protection scope of the present application is not limited thereto. Therefore, any equivalent changes made according to the structure, shape, and principle of the present application should be included in the protection scope of the present application.

Claims

1. A method for intervention in an enriched environment for mice, characterized in that: The following steps are involved: An experimental mouse sample library is selected from a large number of mice, and the mice in the experimental mouse sample library are divided into five equal parts to obtain the mouse sample grouping results; Based on the results of mouse sample grouping, the oxytocin concentration in the control mice was detected to obtain the control oxytocin concentration data; Based on the results of mouse sample grouping, the visual environment of the mice was changed and the effect on the oxytocin concentration of the mice was analyzed to output the results of the visual environment impact; Based on the results of mouse sample grouping, the auditory environment of the mice was changed and the effect on the oxytocin concentration of the mice was analyzed to output the results of the auditory environment impact; Based on the results of mouse sample grouping, the odor environment in which the mice are located is changed and the effect on the oxytocin concentration of the mice is analyzed to output the results of the olfactory environment impact; Based on the results of mouse sample grouping, the touch environment of the mice was changed and the effect on the oxytocin concentration of the mice was analyzed to output the results of the tactile environment impact; Acquiring environmental information of the mouse to be tested, detecting the physical condition of the mouse to be tested to obtain the physical condition information of the mouse, and controlling the concentration of oxytocin produced by the mouse to be tested based on the environmental information of the mouse to be tested, the physical condition information of the mouse, the visual environment impact result, the auditory environment impact result, the olfactory environment impact result, and the tactile environment impact result; The steps of selecting an experimental mouse sample library from a large number of mice, and dividing the mice in the experimental mouse sample library into five equal parts to obtain the mouse sample grouping results specifically include: A large number of mice are obtained to obtain overall information of the mice, and the ages of all the mice are obtained based on the overall information of the mice to obtain the age information of the mice; Comparing the mouse age information with the preset mouse standard age range to obtain the mouse age comparison result; Based on the mouse age comparison result, mice whose age information exceeds a preset mouse standard age range are screened and removed, and mice whose age information is within the preset mouse standard age range are retained to obtain a first mouse screening result; Weighing the mice based on the first mouse screening result to obtain mouse weight information, and comparing the mouse weight information with a preset mouse standard weight range to obtain a mouse weight comparison result; Based on the mouse weight comparison result, mice whose weight information exceeds the preset mouse standard weight range are screened and removed, and mice whose weight information is within the preset mouse standard weight range are retained to obtain a second mouse screening result; Based on the second mouse screening result, the mouse medical history information and the mouse family medical history information are obtained, mice with sequelae diseases in the mouse medical history information and mice with genetic diseases in the mouse family medical history information are screened and removed, and the screened and removed mice are retained to obtain an experimental mouse sample library; Based on the experimental mouse sample library, the mice are divided into five equal parts to obtain a mouse sample grouping result, wherein the mouse sample grouping result includes five sub-sample libraries, namely, a control sub-sample library, a first sub-sample library, a second sub-sample library, a third sub-sample library, and a fourth sub-sample library; Based on the mouse sample grouping results, the step of detecting the oxytocin concentration in the control mouse to obtain the control oxytocin concentration data specifically includes: Acquire a sensitive fluorescent OT sensor and establish a signal connection link between the sensitive fluorescent OT sensor and a control subsample library; The mice in the control sub-sample library were placed in an environment with suitable temperature and humidity, and the oxytocin concentration in the mice in the control sub-sample library was detected in real time based on a sensitive fluorescent OT sensor to obtain the control oxytocin concentration data; Based on the mouse sample grouping results, the steps of changing the visual environment of the mice and analyzing the effect on the oxytocin concentration of the mice to output the visual environment effect results specifically include: Put the mice in the first sub-sample library in the mouse sample grouping result into a first mouse cage, wherein the inner wall of the first mouse cage is provided with an LED display screen; The color hue on the LED display screen is changed in sequence, the color hue retention time of each hue on the LED display screen is a fixed value, the time interval between the color hues of every two hues is a preset color retention time, and the mice are rewarded by supplementing food and water during the color retention time; Based on the sensitive fluorescent OT sensor, the concentration of oxytocin in each hue during the residence time of the color hue is detected to obtain multiple hue oxytocin concentration data; Compare the oxytocin concentrations of multiple hues with the control oxytocin concentration data and calculate the difference to obtain the oxytocin concentration change data of each hue, and compare and sort the oxytocin concentration change data of each hue to obtain the hue influence result; The hue represented by the middle value of the hue influence result is recorded as the designated hue, the color brightness of the designated hue is changed from light to dark on the LED display screen, the body temperature of the mice in the first sub-sample library is detected to obtain the first mouse body temperature information, the first mouse body temperature information is compared with the preset mouse standard body temperature information and the absolute value is taken to obtain the first mouse body temperature difference; Based on the temperature difference of the first mouse, the color brightness change amplitude of the specified hue and the color brightness retention time of each color brightness on the LED display screen are determined, and the color brightness retention time interval between every two brightnesses is the preset color retention time, and the mouse is rewarded by supplementing food and water during the color retention time; Based on the sensitive fluorescent OT sensor, the oxytocin concentration during the color brightness residence time of each brightness is detected to obtain multiple brightness oxytocin concentration data; Compare multiple brightness oxytocin concentration data with control oxytocin concentration data and calculate the difference to obtain oxytocin concentration change data of each brightness, and compare and sort the oxytocin concentration change data of each brightness to obtain brightness influence results; The hue influence result and the brightness influence result are combined to form a visual environment influence result; Based on the mouse sample grouping results, the steps of changing the auditory environment of the mice and analyzing the effect on the oxytocin concentration of the mice to output the auditory environment effect results specifically include: The mice in the second sub-sample library in the mouse sample grouping result are placed in a second mouse cage, a speaker is arranged on the inner wall of the second mouse cage, and different types of sounds are played through the speaker, the type playing time of each type of sound is a fixed value, and the time interval between every two types of sounds is a preset sound retention time, and the mice are rewarded with food and water during the sound retention time; Based on the sensitive fluorescent OT sensor, the oxytocin concentration of the sound type during the sound residence time is detected to obtain multiple sound type oxytocin concentration data, the multiple sound type oxytocin concentration data are compared with the control oxytocin concentration data, and the difference is calculated to obtain the oxytocin change concentration data of each sound type, and the oxytocin change concentration data of each sound type are compared and sorted to obtain the sound type impact result; The sound type represented by the middle value in the sound type influence result is recorded as the designated sound type, the frequency of the designated sound type is played in sequence from low to high based on the speaker, the body temperature of the mice in the second subsample library is detected to obtain the second mouse body temperature information, the second mouse body temperature information is compared with the preset mouse standard body temperature information and the absolute value is taken to obtain the second mouse body temperature difference; Based on the body temperature difference of the second mouse, the frequency change amplitude of the sound type played by the speaker and the sound frequency retention time of each sound frequency are determined, the time interval between the sound frequency retention of every two frequencies is the preset sound retention time, and the mouse is rewarded by supplementing food and water during the sound retention time; Based on the sensitive fluorescent OT sensor, the oxytocin concentration within the sound frequency residence time of different sound frequencies is detected to obtain the oxytocin concentration data of multiple frequencies, the oxytocin concentration data of multiple frequencies are compared with the control oxytocin concentration data and the difference is calculated to obtain the oxytocin concentration change data of each sound frequency, and the oxytocin concentration change data of each sound frequency are compared and sorted to obtain the sound frequency influence result; The sound frequency represented by the middle value in the sound frequency impact result is the designated sound frequency, and the designated sound type and the designated sound frequency are played in order from low to high based on the speaker, and the sound with different sound intensities is played based on the speaker, and the intensity playing time of each sound intensity is a fixed value, and the time interval between the playing of each two intensities is a preset sound retention time, and the mice are rewarded with food and water during the sound retention time; Based on the sensitive fluorescent OT sensor, the oxytocin concentration of different sound intensities during the playing time is detected to obtain multiple sound intensity oxytocin concentration data, the multiple sound intensity oxytocin concentration data are compared with the control oxytocin concentration data and the difference is calculated to obtain the oxytocin change concentration data of each sound intensity, and the oxytocin change concentration data of each sound intensity are compared and sorted to obtain the sound intensity impact result; The sound type influence result, the sound frequency influence result and the sound intensity influence result are combined to form an auditory environment influence result.

2. The method for enriching the environment of mice according to claim 1, characterized in that: Based on the results of mouse sample grouping, the steps of changing the odor environment in which the mice are located and analyzing the effect on the oxytocin concentration of the mice to output the olfactory environment effect results specifically include: Put the mice in the third sub-sample library in the mouse sample grouping result into a third mouse cage, and perform air purification operation on the third mouse cage; Inject air with different odor properties into the third mouse cage, the odor property residence time of the air with different odor properties in the third mouse cage is a fixed value, the time interval between the residence of every two odor properties is a preset odor residence time, and during the odor residence time, food and water are added to reward the mice and the air of the third mouse cage is purified; Based on the sensitive fluorescent OT sensor, the mouse oxytocin concentration when the air with different odor properties was injected into the third mouse cage was detected to obtain multiple odor property oxytocin concentration data; Compare the oxytocin concentration data of multiple odor properties with the control oxytocin concentration data and calculate the difference to obtain the oxytocin concentration change data of each odor property, and compare and sort the oxytocin concentration change data of each odor property to obtain the influence result of the odor property; The odor property represented by the middle value of the odor property influence result is recorded as the designated odor property, the odor concentration of the designated odor property is injected into the third mouse cage, wherein the odor concentration of the designated odor property is from low to high, the body temperature of the mice in the third sub-sample library is detected to obtain the body temperature information of the third mouse, the body temperature information of the third mouse is compared with the preset standard body temperature information of the mouse, and the absolute value is taken to obtain the body temperature difference of the third mouse; Determine the change range of the odor concentration of the specified odor property and the odor concentration residence time of each odor concentration based on the body temperature difference of the third mouse, the odor concentration residence time interval between every two odor concentrations is the preset odor residence time, and reward the mouse with food and water during the odor residence time and perform air purification operation on the third mouse cage; The oxytocin concentration of mice during the odor concentration residence time of each odor concentration was detected based on the sensitive fluorescent OT sensor to obtain multiple odor concentration oxytocin concentration data; Compare the oxytocin concentration data of multiple odor concentrations with the control oxytocin concentration data and calculate the difference to obtain the oxytocin concentration change data of each odor concentration, and compare and sort the oxytocin concentration change data of each odor concentration to obtain the odor concentration influence result; The odor property influence result and the odor concentration influence result are combined to form the olfactory environment influence result.

3. The method for enriching the environment of mice according to claim 2, characterized in that: Based on the results of mouse sample grouping, the steps of changing the touch environment in which the mice are located and analyzing the effect on the oxytocin concentration of the mice to output the tactile environment effect results specifically include: The mice in the fourth sub-sample library in the mouse sample grouping result are placed in a fourth mouse cage, a replacement layer is arranged at the bottom of the fourth mouse cage, and a temperature control layer is arranged below the replacement layer; wherein the replacement layer is provided with a replaceable mouse touch object, and the temperature control layer includes a temperature control plate, and the temperature control plate is used to control the temperature of the mouse touch object; Place touch objects with different smoothness in the replacement layer, the touch object residence time of the touch objects with different smoothness is a fixed value, the touch object residence time of every two touch objects with different smoothness is a preset touch residence time, and the mice are rewarded with food and water during the touch residence time, and the touch object most frequently used by the mice is placed in the replacement layer; Based on the sensitive fluorescent OT sensor, the oxytocin concentration data of mice with touch objects of different smoothness placed on the replacement layer in the fourth mouse cage were detected to obtain multiple touch object oxytocin concentration data; Compare the oxytocin concentration data of multiple touch objects with the oxytocin concentration data of the control and calculate the difference to obtain the oxytocin concentration change data of touch objects with different smoothness, and compare and sort the oxytocin concentration change data of touch objects with different smoothness to obtain the touch object impact result; The touch object represented by the middle value of the touch object influence result is recorded as the designated touch object, the designated touch object is placed in the replacement layer, the temperature of the designated touch object is changed based on the temperature control board, the body temperature of the mice in the fourth sub-sample library is detected to obtain the fourth mouse body temperature information, the fourth mouse body temperature information is compared with the preset mouse standard body temperature information and the absolute value is taken to obtain the fourth mouse body temperature difference value; Determine the temperature change amplitude of the temperature control plate based on the fourth mouse body temperature difference, set the temperature change time interval of the temperature control plate to a preset touch residence time, replenish food and water to reward the mouse during the touch residence time, place the most frequently touched object of the mouse in the replacement layer and adjust the temperature control plate to a suitable temperature for the mouse; Based on the sensitive fluorescent OT sensor, the mouse oxytocin concentration when the designated touch object is at different temperatures is detected to obtain multiple temperature oxytocin concentration data; Compare the oxytocin concentration data at multiple temperatures with the control oxytocin concentration data and calculate the difference to obtain the oxytocin concentration change data at different temperatures, and compare and sort the oxytocin concentration change data at different temperatures to obtain the temperature influence result of the touch object; The touch object influence result and the touch object temperature influence result are combined to form a tactile environment influence result.

4. The method for enriching the environment of mice according to claim 3, characterized in that: The steps of obtaining the environmental information of the mouse to be tested, detecting the physical condition of the mouse to be tested to obtain the physical condition information of the mouse, and controlling the concentration of oxytocin produced by the mouse based on the environmental information of the mouse to be tested, the physical condition information of the mouse, the visual environment impact result, the auditory environment impact result, the olfactory environment impact result, and the tactile environment impact result specifically include: Acquire the environment information of the mouse to be tested, and obtain the complexity of the mouse environment layout based on the environment information of the mouse to be tested, wherein the complexity of the mouse environment layout includes the complexity of the mouse visual environment layout, the complexity of the mouse auditory environment layout, the complexity of the mouse olfactory environment layout, and the complexity of the mouse tactile environment layout; Detecting the physical condition of the mouse to be tested to obtain the physical condition information of the mouse, and judging the sensory perception degree of the mouse based on the physical condition information of the mouse, wherein the sensory perception degree of the mouse includes the visual perception degree of the mouse, the auditory perception degree of the mouse, the olfactory perception degree of the mouse, and the tactile perception degree of the mouse; Determining the mouse perception adjustment items based on the complexity of the mouse environment layout and the mouse sensory perception degree, wherein the mouse perception adjustment items include vision, hearing, smell and touch; The oxytocin concentration produced by mice is regulated based on the mouse's perception regulation items, visual environment influence results, auditory environment influence results, olfactory environment influence results and tactile environment influence results.

5. An intervention system for mouse enrichment environment, characterized in that: The intervention system for enriching the environment of mice is used to implement the intervention method for enriching the environment of mice described in any one of claims 1 to 4, comprising: An experimental mouse selection module is configured to select an experimental mouse sample library from a large number of mice, and divide the mice in the experimental mouse sample library into five equal parts to obtain a mouse sample grouping result; A control group module is configured to detect the oxytocin concentration in the control mice based on the mouse sample grouping results to obtain control oxytocin concentration data; A visual environment analysis module is configured to change the visual environment of the mice based on the mouse sample grouping results, analyze the impact on the oxytocin concentration of the mice, and output the visual environment impact results; An auditory environment analysis module is configured to change the auditory environment of the mice based on the mouse sample grouping results, analyze the impact on the oxytocin concentration of the mice, and output the auditory environment impact results; The olfactory environment analysis module is configured to change the odor environment of the mice based on the mouse sample grouping results, analyze the impact on the oxytocin concentration of the mice, and output the olfactory environment impact results; A tactile environment analysis module is configured to change the touch environment of the mouse based on the mouse sample grouping results, analyze the impact on the mouse oxytocin concentration, and output the tactile environment impact results; The oxytocin regulation module is configured to obtain the environmental information of the mouse to be tested, detect the physical condition of the mouse to be tested to obtain the mouse physical condition information, and control the oxytocin concentration produced by the mouse based on the environmental information of the mouse to be tested, the mouse physical condition information, the visual environment impact results, the auditory environment impact results, the olfactory environment impact results and the tactile environment impact results.

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