Far field electrospinning device, preparation method and application of ordered nanofiber membrane

Abstract

The application belongs to the technical field of nanofiber membrane, and particularly relates to a far-field electrospinning device and a preparation method and application of an ordered nanofiber membrane. The far-field electrospinning device provided in the application introduces an electrospinning metal template, utilizes an uneven electric field of a main structure and a hollow area in the electrospinning metal template to guide deposition of an electrospinning solution, and deposits an ordered nanofiber membrane on a receiving plate. The far-field electrospinning device overcomes the great randomness existing in the fiber deposition process of the current far-field electrospinning device. By setting the cleaning electrospinning metal templates which are mirror images of each other, redundant nanofiber membranes can be removed. By dividing the electrospinning metal template into a plurality of combined layered slice templates, the layered slice templates are used for 'layer-by-layer printing', which is beneficial to improving the precision of the ordered nanofiber membrane. Thus, the technical problem that the electrospinning device is difficult to prepare the ordered nanofiber in the prior art is solved.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of nanofiber membranes, and particularly relates to a far-field electrospinning device and a preparation method and application of an ordered nanofiber membrane. BACKGROUND

[0002] Electrospinning is a method for preparing fibers by using high-voltage static electricity to excite a polymer solution. The polymer used for preparing the fibers is first dissolved in an organic solution and then loaded into a syringe with a pointed liquid outlet. Under the action of a high-voltage static field, the droplets at the liquid outlet gradually change from a spherical shape to a conical shape, and a fine jet is ejected to a receiving plate to form a polymer fiber.

[0003] Electrospinning technology can be divided into near-field electrospinning and far-field electrospinning. Near-field electrospinning technology can form continuous and stable polymer spinning, and the spinning can form an ordered microstructure on the receiving plate. However, the spinning formation process needs the participation of a large number of platform displacement mechanisms, and the fiber aperture obtained by near-field electrospinning technology is relatively large. Currently, near-field electrospinning is mainly used for preparing polymer micrometer fibers. Under the premise that the precision of the driver is limited and the platform mechanism cannot be changed, near-field electrospinning cannot form an unordered pore structure similar to a cell matrix, and therefore cannot be used for batch manufacturing of nanofiber membranes for cell culture. In far-field electrospinning technology, the receiving plate is often far away from the liquid outlet. Under the continuous action of a high-voltage static field, the spinning jet can be fully stretched to form a fiber with a relatively small diameter. The slender spinning is overlapped and entangled with each other, so that the fiber aperture can reach the nanometer scale, and it is more suitable for preparing a nanofiber net with a dense inner layer. Due to the long ejection distance, the polymer spinning formation process is unstable, and the lengths of the formed fibers are different. The fiber morphology prepared by far-field electrospinning is not controllable, and the deposition process has a large randomness. Therefore, it is difficult to form an ordered structure required for cell culture. Therefore, the traditional near-field electrospinning technology is difficult to be used for preparing nanofibers with a small diameter, and the far-field electrospinning technology is difficult to be used for preparing ordered fibers.

[0004] Due to the complexity of human body structure, the existing mechanical artificial organs are difficult to completely simulate the life activities of organs. The organoids obtained based on living cell culture have good biocompatibility and have application prospects for organ transplantation. In the culture process of the organoids, the extracellular matrix and the nutrient substrate have a great effect. Research shows that, on the one hand, the culture substrate with a specific microstructure can promote the growth and differentiation of cells, and narrow the gap between in vitro cultured cells and in vivo cells; on the other hand, the growth scaffold needs to provide a suitable culture environment for the life activities of cells. If the pore size of the scaffold is too large, the cells cannot be fixed, and if the pore size is too small, the nutrient substances cannot be transported in time. Therefore, the preparation of tissue engineering scaffold materials by using micro-nano manufacturing technology is of great significance to the development of regenerative medicine. Compared with photolithography, hot pressing and other micro-nano manufacturing technologies, the far-field electrospinning manufacturing technology has low cost and high efficiency. The nanofiber membrane prepared by the far-field electrospinning manufacturing technology has a nanoscale fiber diameter and pore size, and can fix cells, induce the growth direction of cells and transport nutrient substances in cell culture, and has the potential to be applied in the technical field of biological tissue engineering.

[0005] However, the traditional electrospinning such as far-field electrospinning is difficult to prepare ordered nanofibers, and cannot meet the requirements of tissue engineering scaffold materials. Therefore, there is a need to improve the far-field electrospinning technology. SUMMARY

[0006] Therefore, the application provides a far-field electrospinning device and a preparation method and application of an ordered nanofiber membrane, to solve the technical problem that the electrospinning device in the prior art is difficult to prepare ordered nanofibers.

[0007] The first aspect of the application provides a far-field electrospinning device, which comprises an electrospinning solution injector, a high-voltage power supply, a receiving plate and an electrospinning metal template.

[0008] The positive electrode of the high-voltage power supply is connected to the electrospinning solution injector, and the negative electrode is connected to the electrospinning metal template.

[0009] The receiving plate is located above the electrospinning metal template, and the geometric centers of the receiving plate and the electrospinning metal template are on the same vertical line.

[0010] Preferably, the far-field electrospinning device further comprises a cleaning electrospinning solution injector, and the electrospinning solution injector and the cleaning electrospinning solution injector are located on the same plane.

[0011] The far-field electrospinning device further comprises a cleaning electrospinning metal template, and the main structure region of the electrospinning metal template corresponds to the hollow region of the cleaning electrospinning metal template.

[0012] The hollowed-out area of ​​the electrospinning metal template corresponds to the main structural area of ​​the cleaning electrospinning metal template;

[0013] The receiving plate is located above the cleaning electrospinning metal template, and the geometric centers of the receiving plate and the cleaning electrospinning metal template are on the same vertical line;

[0014] The second positive terminal of the high-voltage power supply is connected to the cleaning electrospinning solution syringe, and the second negative terminal is connected to the cleaning electrospinning metal template.

[0015] Preferably, the far-field electrospinning device further includes a second cleaning electrospinning metal template;

[0016] The length, width, and height of the second cleaning electrospinning metal template are not less than the length, width, and height of the electrospinning metal template;

[0017] The receiving plate is located above the second cleaning electrospinning metal template, and the geometric centers of the receiving plate and the second cleaning electrospinning metal template are on the same vertical line;

[0018] The third positive terminal of the high-voltage power supply is connected to the cleaning electrospinning solution syringe, and the third negative terminal is connected to the second cleaning electrospinning metal template.

[0019] Preferably, the far-field electrospinning device further includes a liquid supply linear motor, which is connected to the syringe and used to control the speed of the syringe.

[0020] Preferably, the far-field electrospinning device further includes a fiber morphology linear motor connected to the receiving plate for controlling the distance between the receiving plate and the syringe.

[0021] Preferably, the far-field electrospinning apparatus further includes a dryer, which is located on the same plane as the receiving plate and is used to dry the electrospinned fibers deposited on the receiving plate.

[0022] The second aspect of this application provides a method for preparing an ordered nanofiber membrane. The method utilizes the far-field electrospinning apparatus described in the first aspect to prepare the ordered nanofiber membrane. The preparation method includes:

[0023] Step S1: Place the electrospinning metal template below the receiving plate in the far-field electrospinning device, wherein the geometric centers of the receiving plate and the electrospinning metal template in the far-field electrospinning device are on the same vertical line.

[0024] Step S2: Connect the positive terminal of the high-voltage power supply in the far-field electrospinning device to the electrospinning solution injector, and the negative terminal to the electrospinning metal template.

[0025] Step S3: Start the far-field electrospinning device and use the unequal electric fields of the main structure region and the hollow region in the electrospinning metal template to guide the deposition of the electrospinning solution, and obtain an ordered nanofiber membrane with the corresponding pattern of the main structure region in the electrospinning metal template on the receiving plate.

[0026] Preferably, after step S3, the following step is also included:

[0027] Step S4: Connect the second positive terminal of the high voltage power supply in the far-field electrospinning device to the cleaning electrospinning solution syringe, and connect the negative terminal to the cleaning electrospinning metal template.

[0028] Step S5: Start the far-field electrospinning device, and use the unequal electric field between the main structure region and the hollow region in the cleaning electrospinning metal template to guide the deposition of the cleaning electrospinning solution, remove the redundant nanofiber membrane in the hollow region of the electrospinning metal template on the receiving plate, and obtain an ordered nanofiber membrane.

[0029] Preferably, after step S3, the following step is also included:

[0030] Step S41: Connect the third positive terminal of the high voltage power supply in the far-field electrospinning device to the cleaning electrospinning solution syringe, and connect the third negative terminal to the second cleaning electrospinning metal template.

[0031] Step S51: Start the far-field electrospinning device, use the second cleaning electrospinning metal template to guide the cleaning electrospinning solution to be deposited on the receiving plate, remove the redundant nanofiber membrane in the hollow area of ​​the electrospinning metal template on the receiving plate, and obtain an ordered nanofiber membrane.

[0032] The electrospinning time in the main structural region of the electrospinning metal template Length of the main structural region ,width and height The second cleaning electrospinning metal template guides the deposition time of the cleaning electrospinning solution. Electrospinning solution injector liquid supply speed Cleaning electrospinning solution syringe liquid supply speed The planar area of ​​the main structural region and the overall planar area of ​​the electrospinning metal template. The following conditions, as shown in Equation 1, must be satisfied;

[0033] Formula 1.

[0034] Preferably, after step S5, the following step is also included:

[0035] Step S6: Repeat steps S1 to S5 to obtain a multilayer ordered nanofiber membrane.

[0036] Preferably, in step S1, the method for preparing the electrospinning metal template includes:

[0037] Step 1A: Construct a 3D model of the electrospinning metal template using computer software;

[0038] Step 1B: Import the 3D model of the electrospun metal template into a metal 3D printer for 3D printing to obtain the electrospun metal template.

[0039] Preferably, in step S4, the method for preparing the cleaning electrospinning metal template includes:

[0040] Step 4A: Construct a mirrored 3D model of the electrospinning metal template using computer software;

[0041] Step 4B: Import the mirrored 3D model of the electrospun metal template into the metal 3D printer for 3D printing to obtain the cleaned electrospun metal template.

[0042] Preferably, the computer software is selected from 3DMAX computer software;

[0043] The metal 3D printer is selected from selective laser sintering metal 3D printers.

[0044] Preferably, in step S1, the method for preparing the electrospinning metal template includes:

[0045] Step 1Aa: Construct a 3D model of the electrospinning metal template using computer software;

[0046] Step 1Ba: Create at least two layered slice models of the electrospinning metal template based on the 3D model of the electrospinning metal template.

[0047] Step 1Ba: Import the layered slice model of at least two electrospun metal templates into a metal 3D printer one by one for 3D printing to obtain layered slice templates of at least two electrospun metal templates.

[0048] Step 1Bb: Combine the layered slice templates of at least two electrospinning metal templates to obtain an electrospinning metal template.

[0049] Preferably, in step S4, the method for preparing the cleaning electrospinning metal template includes:

[0050] Step 4Aa: Construct a mirrored 3D model of the cleaning electrospinning metal template using computer software;

[0051] Step 4Ba: Create at least two layered slice mirror models of the cleaning electrospinning metal template based on the mirror 3D model of the cleaning electrospinning metal template.

[0052] Step 4Ba: Import the layered slice mirror model of at least two cleaning electrospinning metal templates into the metal 3D printer one by one for 3D printing to obtain at least two layered slice mirror templates of cleaning electrospinning metal templates.

[0053] Step 4Bb: Combine at least two layered slice templates of the cleaning electrospinning metal template to obtain the cleaning electrospinning metal template.

[0054] The third aspect of this application provides the application of ordered nanofiber membranes in the preparation of tissue engineering scaffold materials.

[0055] In summary, this application provides a far-field electrospinning device and a method for preparing and applying ordered nanofiber membranes. The far-field electrospinning device includes an electrospinning solution injector, a high-voltage power supply, a receiving plate, and an electrospinning metal template. The positive and negative terminals of the high-voltage power supply are connected to the electrospinning solution injector and the electrospinning metal template, respectively. The electrospinning solution injector sprays a fine electrospinning solution onto the receiving plate to form a nanofiber membrane. The electrospinning metal template is positioned below the receiving plate and on the same vertical line as the geometric center of the receiving plate. This allows the electrospinning metal template to guide electrostatics using the asymmetrical electric fields between the main structural region and the hollowed-out region. The spinning solution is deposited in an orderly manner on the corresponding main structure region of the receiving plate to obtain an ordered nanofiber membrane. Furthermore, redundant fibers deposited in the hollowed-out areas are removed by cleaning the electrospinning metal template, which is a mirror image of the electrospinning metal template, thus improving the precision of the ordered nanofiber membrane. Additionally, by dividing the electrospinning metal template into multiple combined layered slicing templates, and then "printing" layer by layer using these templates before assembly, the single-cycle working time of electrospinning is reduced, which is beneficial for improving the precision of the ordered nanofiber membrane. The prepared ordered nanofibers can meet the requirements of tissue engineering scaffold materials. This solves the technical problem of the difficulty in preparing ordered nanofibers using existing electrospinning devices. Attached Figure Description

[0056] To more clearly illustrate the technical solutions in the specific embodiments of this application or the prior art, the drawings used in the description of the specific embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of this application. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0057] Figure 1 This is a schematic diagram of the far-field electrospinning apparatus provided in Embodiment 1 of this application;

[0058] Figure 2 A schematic diagram of the spinning process using a far-field electrospinning device is provided for Embodiment 3 of this application;

[0059] Figure 3 This is a schematic diagram showing the process before and after cleaning redundant fibers deposited in the hollowed-out area of ​​the electrospinning metal template using a far-field electrospinning device, as provided in Embodiment 3 of this application. Detailed Implementation

[0060] This application provides a far-field electrospinning device and a method for preparing ordered nanofiber membranes, as well as their applications, to solve the technical problem that existing electrospinning devices are difficult to use for preparing ordered nanofibers.

[0061] The technical solutions of this application will now be clearly and completely described with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of this application. Based on the embodiments of this application, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this application.

[0062] Currently, far-field electrospinning devices mainly include a high-voltage power supply, a syringe storing the electrospinning solution, and a receiving plate. The positive and negative terminals of the high-voltage power supply are connected to the syringe and the receiving plate, respectively. Under the action of the high-voltage electrostatic field, the droplets at the syringe outlet gradually change from a spherical shape to a pointed cone shape, and the fine jet is sprayed onto the receiving plate to form polymer fibers. However, the polymer spinning process is unstable, and the resulting fibers have varying lengths and sizes. The morphology of fibers prepared by far-field electrospinning is not controllable, and the deposition process has a large degree of randomness. Therefore, current far-field electrospinning devices have the defect of being unable to prepare ordered nanofiber membranes. In view of this, this application provides a far-field electrospinning device. The far-field electrospinning device provided in this application introduces an electrospinning metal template, and the receiving plate is located at the electrospinning... Above the electrospinning metal template, the geometric centers of the receiving plate and the electrospinning metal template are on the same vertical line. After the electrospinning metal template and the syringe storing the electrospinning solution are connected to a high-voltage power supply, a high-voltage electrostatic field exists between the syringe storing the electrospinning solution and the electrospinning metal template in the far-field electrospinning device. Due to the main structural region composed of the formed metal pattern and the hollow region without formed metal in the electrospinning metal template, the unequal electric field between the main structural region and the hollow region in the electrospinning metal template can guide the deposition of the electrospinning solution. An ordered nanofiber membrane corresponding to the pattern in the main structural region of the electrospinning metal template is obtained on the receiving plate. This overcomes the defect that the fiber deposition process of the current far-field electrospinning device has a large degree of randomness and cannot prepare an ordered nanofiber membrane.

[0063] As a preferred method, to remove a small amount of redundant spinning fibers accidentally deposited in the hollowed-out areas of the electrospinning metal template, this application provides a technical solution for cleaning a small amount of redundant spinning fibers that may exist in the hollowed-out areas. The first technical solution utilizes a cleaning electrospinning metal template that is a mirror image of the electrospinning metal template, along with a cleaning electrospinning solution syringe, to clean the small amount of redundant spinning fibers that may exist in the hollowed-out areas. Specifically, the preparation of the electrospinning metal template and the cleaning electrospinning metal template can be achieved using 3DMAX computer software and a selective laser sintering metal 3D printer. 3DMAX is a three-dimensional animation rendering and production software that can construct a three-dimensional model of the electrospinning metal template and a corresponding mirrored three-dimensional model (the cleaning electrospinning metal template), i.e., the main structural area composed of the formed metal pattern in the three-dimensional model of the electrospinning metal template and the cleaning electrospinning metal template. The hollow areas without shaped metal in the 3D model of the plate correspond to the hollow areas without shaped metal in the 3D model of the electrospun metal template and the main structural areas composed of shaped metal patterns in the 3D model of the cleaning electrospun metal template. After constructing the 3D models of the electrospun metal template and the cleaning electrospun metal template, they can be imported into a selective laser sintering metal 3D printer to prepare the electrospun metal template and the cleaning electrospun metal template. Since the cleaning electrospun metal template and the electrospun metal template are mirror images of each other, the main structural areas of the cleaning electrospun metal template correspond to the hollow areas of the electrospun metal template. After replacing the electrospun metal template with the cleaning electrospun metal template, the asymmetrical electric field between the main structural areas and the hollow areas in the cleaning electrospun metal template can guide the solvent sprayed by the cleaning electrospun solution syringe to deposit on the hollow areas of the spun fiber membrane, effectively dissolving and removing any possible small amount of redundant spun fibers.

[0064] As a preferred option, considering that if the length, width, and height of the ordered nanofiber membrane are too large, the corresponding electrospinning metal template would also be too large, which would be detrimental to improving the precision of electrospinning, this application also adopts a technical solution of combining a layered slicing template with an electrospinning metal template. This allows the electrospinning metal template to be divided into at least two layered slicing templates; and sets the model printing resolution. The number of slice layers n, the height h of the 3D model, and the length of each slice model. ,width and height Based on the 3D model, a layered slice model of the nanofiber membrane was made, and the solid area ratio of each layered slice model was obtained.

[0065] Model printing resolution refers to the minimum acceptable printing precision of the nanofiber membrane during printing. A layered slicing model has n layers, and the height of the i-th layer is... 'i' represents the number of the slice model at that layer (the bottom slice model is numbered 1, and the numbers increase sequentially from bottom to top, with the top slice model numbered n). The following requirements should be met:

[0066]

[0067] Where h is the total height of the 3D model of the nanofiber membrane.

[0068] The planar area of ​​the main structural region and the overall planar area of ​​the i-th layer slice model of the electrospinning metal template can be calculated according to the following formula.

[0069]

[0070] In the formula, x represents the horizontal coordinate of a point on the main structure of the i-th layer slice model; y represents the vertical coordinate of a point on the main structure of the i-th layer slice model.

[0071] Regarding the technical solution of combining layered slicing templates with electrospinning metal templates, since there are at least two layered slicing templates, if a cleaning electrospinning metal template mirrored by the electrospinning metal template and a cleaning electrospinning solution syringe are used to clean the hollowed-out area where there may be a small amount of redundant spinning fibers, a large number of layered slicing templates would be required due to the differences in the structure of the layered slicing templates. Therefore, this application also provides a second technical solution for cleaning the hollowed-out area where there may be a small amount of redundant spinning fibers. This method uses a second cleaning electrospinning metal template, and cleans the hollowed-out area using a cleaning electrospinning solution syringe based on the second cleaning electrospinning metal template and according to preset parameters. The spinning time of the main structural area in the electrospinning metal template is preset according to the far-field electrospinning device. Length of the main structural region ,width and height The second cleaning electrospinning metal template guides the deposition time of the cleaning electrospinning solution. Electrospinning solution injector liquid supply speed Cleaning electrospinning solution syringe liquid supply speed The planar area of ​​the main structural region and the overall planar area of ​​the electrospinning metal template. The following conditions, as shown in the formula below, are met to ensure that redundant fibers in the hollowed-out area are cleaned thoroughly, while the fibers in the main structural area are not excessively damaged.

[0072]

[0073] Since the parameters are set to ensure that the redundant fibers in the hollow area are cleaned, while the fibers in the main structure area are not excessively damaged, the second cleaning electrospinning metal template only needs to ensure that its length, width, and height are not lower than the length, width, and height of the layered slicing template.

[0074] Example 1

[0075] Embodiment 1 of this application provides a far-field electrospinning device, the structure of which is as follows: Figure 1 As shown, it includes: an electrospinning solution injector 1, a cleaning electrospinning injector 2, a high-voltage power supply, a receiving plate, and a metal template, as well as linear motors 1-3 and a dryer.

[0076] In the far-field electrospinning device, the metal templates include an electrospinning metal template and a cleaning electrospinning metal template. The main structural area of ​​the electrospinning metal template corresponds to the hollow area of ​​the cleaning electrospinning metal template, and the hollow area of ​​the electrospinning metal template corresponds to the main structural area of ​​the cleaning electrospinning metal template. This makes the electrospinning metal template and the cleaning electrospinning metal template mirror images of each other. The main structural area refers to the area in the metal template where the metal is formed, and the hollow area refers to the area without metal.

[0077] Electrospinning solution injector 1 is used to store electrospinning solution, and cleaning electrospinning injector 2 is used to store solvent in electrospinning solution. The needles of electrospinning solution injector 1 and cleaning electrospinning injector 2 are in the same plane, and linear motors 1-2 are respectively connected to electrospinning solution injector 1 and cleaning electrospinning injector 2, thereby controlling the liquid supply speed of injectors 1-2.

[0078] The receiving plate and the metal template constitute the spinning platform. Specifically, the metal template is positioned below the receiving plate, and the geometric centers of the receiving plate and the electrospinning metal template are on the same vertical line. This allows the spinning solution or solvent to be guided to deposit at a specific location when the electrospinning solution injector 1 and the storage container 2 supply the solution.

[0079] When the high-voltage power supply is connected to the electrospinning solution injector 1 and the electrospinning metal template in the metal template, respectively, the asymmetrical electric field between the main structural region and the hollowed-out region can be used to guide the electrospinning solution to be deposited in an orderly manner in the main structural region of the electrospinning metal template, and then dried by a dryer. When the high-voltage power supply is connected to the cleaning electrospinning solution injector 2 and the cleaning electrospinning metal template in the metal template, respectively, the asymmetrical electric field between the main structural region and the hollowed-out region can be used to guide the cleaning electrospinning solution to be deposited in an orderly manner in the main structural region of the cleaning electrospinning metal template, and remove redundant fibers accidentally deposited in the hollowed-out region.

[0080] The linear motor 3, connected to the spinning platform, can control the position between the receiving plate and the electrospinning solution injector 1 and the cleaning electrospinning solution injector 2, so as to control the size of the spun fibers deposited on the receiving plate.

[0081] Example 2

[0082] Embodiment 2 of this application provides a far-field electrospinning device. The difference between this far-field electrospinning device and Embodiment 1 is that the electrospinning metal template is set as a combination of three layered slice models. The size settings of the three layered slice models of the electrospinning metal template are shown below.

[0083]

[0084] And the height of the three slices meets the requirements. The ratio of the solid areas of the three slice models can be obtained by calculation. , , .

[0085] Example 3

[0086] Embodiment 3 of this application provides a method for electrospinning using the far-field electrospinning apparatus described in Embodiment 2, which can prepare ordered nanofiber membranes. The method includes:

[0087] Step S1: Construct a 3D model of the electrospinning metal template using 3DMAX, and set the model printing resolution. Given the number of slicing layers n=3, the 3D model height h=3mm, and the length, width, and height dimensions of each slice model, a layered slicing model for the electrospinning metal template is created based on the 3D model; the dimensions of the three-layer slicing model are as follows:

[0088]

[0089] And the height of the three slices meets the requirements. The ratio of the solid areas of the three slice models can be obtained by calculation. , , .

[0090] Step S2: Based on the layered slice model of the microstructure, import it into the selective laser sintering metal 3D printer to create three corresponding electrospinning metal templates, and sort the electrospinning metal templates for subsequent electrospinning.

[0091] Step S3: Take polylactic acid with a molecular weight of 80kJ and prepare a 10% PLA solution as the main structure electrospinning solution using a mixture of dichloromethane and N,N-dimethylacetamide (volume ratio 9:1) as the solvent. Separately prepare a 7wt% N,N-dimethylacetamide solution as the cleaning electrospinning solution. Pour the prepared electrospinning solution into a 50mL electrospinning syringe 1 and the cleaning electrospinning solution into a 50mL cleaning electrospinning syringe 2. Install both solutions on the preparation apparatus, setting the electric field voltage to 20kV, the receiving plate height to 10cm, and the supply rate of both the electrospinning and cleaning solutions to 5mL / min. The required main structure spinning time and the required impurity removal and cleaning time for each slice can be calculated.

[0092]

[0093]

[0094] Step S4: Place the first electrospinning metal template on the lower layer of the spinning platform. Start the linear motor 3 to drive the spinning platform to move directly below the syringe 1. Connect the negative electrode to the conductive part of the electrospinning metal template and the positive electrode to the needle tip of the syringe 1. Power on and start the linear motor 1 to cause the syringe 1 to extrude the main structure spinning solution at a given liquid supply rate, thus starting electrospinning and simultaneously starting the timer.

[0095] Step S5: When the energizing time reaches the spinning time of the main structure area in the slice model of this layer, disconnect the circuit to stop the timing, turn off the linear motor 1, remove the i-th metal template, install the cleaning electrospinning metal template on the lower layer of the spinning platform, start the linear motor 3 to drive the spinning platform to move directly below the syringe 2, and ensure that the length, width and height of the cleaning electrospinning metal template are not lower than the length, width and height of the layer slice template.

[0096] Step S6: Connect the positive electrode to the tip of the syringe 2, energize and start the linear motor 2, so that the syringe 2 squeezes out the cleaning solution for spinning at a given liquid supply speed, and starts cleaning of redundant fibers, while resetting the timer from zero.

[0097] Step S7: When the energizing time reaches the redundant fiber cleaning time required for the slice model of this layer, disconnect the circuit to stop the timing, turn off the linear motor 2, remove the second cleaning electrospinning metal template, and turn on the air dryer to quickly dry the nanofiber membrane on the receiving plate.

[0098] Step S8: If all metal templates have been used, the spinning process ends, the receiving plate is removed, and the final product is separated to obtain an ordered nanofiber membrane.

[0099] The above embodiments are only used to illustrate the technical solutions of this application, and are not intended to limit them. Although this application has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some or all of the technical features therein. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of this application.

Claims

1. A far-field electrospinning device, characterized in that, include: Electrospinning solution injector, high-voltage power supply, receiving plate, and electrospinning metal template; The positive terminal of the high-voltage power supply is connected to the electrospinning solution injector, and the negative terminal is connected to the electrospinning metal template. The receiving plate is located above the electrospinning metal template, and the geometric centers of the receiving plate and the electrospinning metal template are on the same vertical line; The far-field electrospinning device also includes a syringe for cleaning the electrospinning solution. The far-field electrospinning device also includes a cleaning electrospinning metal template, wherein the main structural area of ​​the electrospinning metal template and the hollow area of ​​the cleaning electrospinning metal template correspond to each other. The hollowed-out area of ​​the electrospinning metal template corresponds to the main structural area of ​​the cleaning electrospinning metal template; The receiving plate is located above the cleaning electrospinning metal template, and the geometric centers of the receiving plate and the cleaning electrospinning metal template are on the same vertical line; The second positive terminal of the high-voltage power supply is connected to the cleaning electrospinning solution syringe, and the second negative terminal is connected to the cleaning electrospinning metal template.

2. The far-field electrospinning device according to claim 1, characterized in that, The far-field electrospinning device also includes a liquid supply linear motor, a fiber morphology linear motor, and an air dryer. The linear motor for liquid supply is connected to the syringe and is used to control the speed of the syringe; The fiber morphology linear motor is connected to the receiving plate and is used to control the distance between the receiving plate and the syringe; The air dryer and the receiving plate are located on the same plane and are used to dry the electrospun fibers deposited on the receiving plate.

3. A method for preparing ordered nanofiber membranes using a far-field electrospinning apparatus as described in any one of claims 1-2, characterized in that, include: Step S1: Place the electrospinning metal template below the receiving plate in the far-field electrospinning device, wherein the geometric centers of the receiving plate and the electrospinning metal template in the far-field electrospinning device are on the same vertical line. Step S2: Connect the positive terminal of the high-voltage power supply in the far-field electrospinning device to the electrospinning solution injector, and the negative terminal to the electrospinning metal template. Step S3: Start the far-field electrospinning device and use the unequal electric fields of the main structure region and the hollow region in the electrospinning metal template to guide the deposition of the electrospinning solution, and obtain an ordered nanofiber membrane with the corresponding pattern of the main structure region in the electrospinning metal template on the receiving plate.

4. The method for preparing an ordered nanofiber membrane according to claim 3, characterized in that, Following step S3, the following steps are also included: Step S4: Connect the second positive terminal of the high voltage power supply in the far-field electrospinning device to the cleaning electrospinning solution syringe, and connect the negative terminal to the cleaning electrospinning metal template. Step S5: Start the far-field electrospinning device, and use the unequal electric field between the main structure region and the hollow region in the cleaning electrospinning metal template to guide the deposition of the cleaning electrospinning solution, remove the redundant nanofiber membrane in the hollow region of the electrospinning metal template on the receiving plate, and obtain an ordered nanofiber membrane.

5. The method for preparing an ordered nanofiber membrane according to claim 3, characterized in that, In step S1, the method for preparing the electrospinning metal template includes: Step 1A: Construct a 3D model of the electrospinning metal template using computer software; Step 1B: Import the 3D model of the electrospun metal template into a metal 3D printer for 3D printing to obtain the electrospun metal template.

6. The method for preparing an ordered nanofiber membrane according to claim 4, characterized in that, In step S4, the method for preparing the cleaning electrospinning metal template includes: Step 4A: Construct a mirrored 3D model of the electrospinning metal template using computer software; Step 4B: Import the mirrored 3D model of the electrospun metal template into the metal 3D printer for 3D printing to obtain the cleaned electrospun metal template.

7. The application of the ordered nanofiber membrane prepared by the preparation method shown in any one of claims 3-6 in the preparation of tissue engineering scaffold materials.

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