Wireless portable small cell culture box

Abstract

The application relates to a wireless portable small cell culture box, which comprises a box body, a control module and a power supply, a culture cavity for placing a cell culture dish and a control chamber for arranging the control module are arranged in the box body; the top and the bottom of the culture cavity corresponding to the box body are transparent; a detection module and an environment adjusting module which are connected with the control module are arranged in the control chamber; the detection module is used for detecting temperature, humidity and bacterial content information in the culture cavity and transmitting the information to the control module; the control module controls the environment adjusting module according to the temperature and the bacterial content information in the culture cavity, so that the temperature and the bacterial content in the culture cavity are ensured to be at set values; the power supply is electrically connected with the control module, the detection module and the environment adjusting module; through the above technical scheme, the application has the advantages of being small, ensuring stable growth of cells, reducing external environmental interference, reducing cross contamination, obtaining accurate and repeatable experimental results and being convenient for automatic operation.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of cell culture, and particularly relates to a wireless portable small cell culture box. BACKGROUND

[0002] Cell experiments are conducted to study and understand the physiological, biochemical and genetic characteristics and functions of cells, and are one of important research means in the fields of biology and medicine, and are widely applied to disease research, biological research, immunological research and cell engineering, etc. In order to scientific research and medical development, a large number of cell culture experiments need to be conducted.

[0003] In the current laboratory, cell culture is mainly collective culture, that is, cells are placed in culture dishes to form cell monolayers or multilayers, and multilayer culture dishes are placed in a culture box to be cultured and researched. In collective culture, the environment in the culture box is not uniform, and it is impossible to ensure that the temperature, humidity, gas environment and other conditions of each layer of culture dishes are completely consistent, which increases the heterogeneity of cell phenotypes and greatly affects the reproducibility of cell experiments.

[0004] In addition, during the process of cell culture, culture dishes need to be frequently taken out for observation. By observing the morphological, size, color and other characteristic changes of cells, the state of cell growth and differentiation can be understood, and the time for processing can be determined in time by finding that cells may mutate or vary. However, each time the culture dish is taken out for observation will expose the cells and culture to the external environment, increasing the risk of contamination by bacteria, fungi, viruses and other microorganisms, and further possibly leading to the failure or variation of cell culture. Taking out the culture dish needs to open the culture box or incubator, which interrupts the growth and replication of cells in a constant environment. Cells cause stress reaction under the condition of changes in external environment and rapid changes in temperature and humidity, which may lead to cell damage, apoptosis or functional abnormalities, and finally lead to uncertainty of the experiment. SUMMARY

[0005] In order to solve the problems in the related art, the application provides a wireless portable small cell culture box, which solves the problems of environmental changes affecting normal growth and differentiation of cells and cells cultured in different growth environments affecting the accuracy of experimental results.

[0006] The technical scheme is as follows:

[0007] The utility model provides a wireless portable small cell culture box, its characteristics are including box, control module and power, the culture cavity for placing cell culture dish and the control room for setting up control module are set up in the box, the culture cavity is correspondingly transparently set up in the top and bottom of the box, the detection module and the environmental regulation module who respectively with control module are set up in the control room, the detection module is used for detecting the temperature, humidity and bacterial content information in the culture cavity and passes temperature, humidity and bacterial content information to control module, and control module controls environmental regulation module work according to temperature and bacterial content information in the culture cavity, ensures that the temperature and bacterial content in the culture cavity are at set value,

[0008] The power supply is a mobile power supply, which is electrically connected with the control module, the detection module and the environmental regulation module, and is used for providing working power for the control module, the detection module and the environmental regulation module.

[0009] Through the above technical scheme, by the transparent setting of the top and bottom of the box, when it is necessary to observe the cultured cells in the middle, the cell culture dish does not need to be taken out, and the cells can be directly observed through the transparent top and bottom of the box, so that the growth environment of the subsequent cell culture can be ensured not to change, thereby avoiding the influence of environmental change on experimental data. At the same time, the control module obtains the temperature, humidity and bacterial content information in the culture cavity, and controls the environmental regulation module to adjust the temperature and bacterial content according to the information, so that all the cells in the culture container can be accurately ensured to be in the same growth environment, and all the cells can be ensured to be in the normal growth or differentiation state, thereby ensuring the reliability of the cell data.

[0010] Further, a base for charging the mobile power supply is further included, the top end of the base is provided with a clamping mechanism and an inflation mechanism, the bottom of the box corresponding to the culture cavity is provided with an inflation port, the clamping mechanism is used to realize the connection of the box and the base, so that the inflation port and the inflation mechanism are connected.

[0011] Further, the box includes a cover plate rotatably arranged on the top of the culture cavity, the cover plate includes a support frame and a glass arranged in the support frame, the environmental regulation module includes a first heating plate and a second heating plate, the first heating plate is fixedly installed in the support frame below the glass in a transparent manner, and the second heating plate is fixedly installed at the bottom of the box corresponding to the culture cavity in a transparent manner.

[0012] Further, an air inlet communicating with the inflation port is arranged on one side vertical surface in the culture cavity, an air outlet is arranged on the other side vertical surface in the culture cavity, and a breathing valve is arranged on the air inlet and the air outlet respectively.

[0013] Further, the front end and the rear end of the box are provided with clamping openings for facilitating the gripping of the mechanical hand.

[0014] Further, a moisturizing groove is arranged along the periphery of the inner bottom of the culture chamber, and a culture dish placement rack is arranged on the periphery of the inner wall of the culture chamber above the moisturizing groove.

[0015] Further, the control module comprises a touch screen arranged on the top of the box in the control chamber, a communication interface chip U37 and a main control chip arranged in the control chamber; the communication interface chip U37 and the main control chip are adaptively connected, the touch screen realizes communication interaction through the communication interface chip U37 and the main control chip; the environment adjusting module comprises two UVC ultraviolet lamps arranged in the culture chamber, and each of the two UVC ultraviolet lamps is connected with a communication isolation unit; the main control chip controls the opening and closing of the two UVC ultraviolet lamps by controlling the two communication isolation units.

[0016] Further, the main control chip is adaptively connected with a 485 communication unit one and a 485 communication unit two; the first heating plate and the second heating plate are respectively connected with a temperature control unit one and a temperature control unit two, the temperature control unit one is connected with the port P7 of the 485 communication unit one, and the temperature control unit two is connected with the port P14 of the 485 communication unit two; the main control chip controls the 485 communication unit one and the 485 communication unit two to work, so as to control the opening and closing of the first heating plate and the second heating plate.

[0017] Further, a one-key switch and a sterilization switch are arranged on one side of the top of the box, the one-key switch is connected with the control module, the detection module and the environment adjusting module, and the sterilization switch is connected with the UVC ultraviolet lamp.

[0018] Further, the base is provided with a gas path pipeline for providing a gas source for the inflation mechanism.

[0019] In summary, the beneficial effects of the present application are that, by arranging the top and bottom of the box to be transparent, when it is necessary to observe the cultured cells in the middle, the cells can be directly observed through the transparent top and bottom of the box without taking out the cell culture dish, so that the growth environment of the subsequent cell culture can be ensured not to change, thereby avoiding the influence of environmental changes on experimental data. At the same time, the control module obtains the temperature, humidity and bacterial content information in the culture chamber, and adjusts the temperature and bacterial content according to the information, so as to accurately ensure that all cells in the culture container are in the same growth environment, ensure that all cells are in a normal growth or differentiation state, and ensure the reliability of cell data.

[0020] It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the application, as claimed. BRIEF DESCRIPTION OF DRAWINGS

[0021] The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments consistent with the application and serve to explain the principles of the application, in which:

[0022] Figure 1 is a structural schematic diagram of the box in the present application;

[0023] Figure 2 is a top view of the box in the present application;

[0024] Figure 3 is a Figure 2 is a sectional view in the A-A direction in the present application;

[0025] Figure 4 is a structural schematic diagram of the box in the present application;

[0026] Figure 5 is a structural schematic diagram of the base in the present application;

[0027] Figure 6 is a schematic diagram of the inside of the culture chamber in the present application;

[0028] Figure 7 is a schematic block diagram of the control module in the present application;

[0029] Figure 8 is an internal schematic diagram of the main control chip in the present application;

[0030] Figure 9 is an internal connection schematic diagram of the 485 communication unit one and the 485 communication unit two in the present application;

[0031] Figure 10 is an internal connection schematic diagram of the touch screen in the present application;

[0032] Figure 11 is an internal connection schematic diagram of the UVC ultraviolet lamp in the present application;

[0033] In the figure, 1, box; 2, base; 3, culture chamber; 4, control chamber; 5, cover plate; 501, frame; 502, glass one; 6, clamping mechanism; 7, air inlet; 8, breather valve; 9, clamping port; 10, culture vessel mounting rack; 11, touch screen; 12, one-key switch; 13, sterilization button; 14, first heating plate; 15, second heating plate; 16, humidification tank. DETAILED DESCRIPTION

[0034] The exemplary embodiments will be described in detail herein with reference to the attached drawings. The description of the exemplary embodiments is intended to apply to all alternative modifications and embodiments as would be apparent to those skilled in the art. It is to be understood that the exemplary embodiments are merely examples and are not intended to limit the scope of the present application. Therefore, specific dimensions and other parameters should not be understood as limiting the present application.

[0035] In one possible embodiment, as shown in the accompanying drawings, Figures 1-11 A wireless portable small cell culture box includes a box body 1, a control module and a power supply, a culture chamber 3 for placing a cell culture dish and a control chamber 4 for setting the control module are arranged in the box body 1; the culture chamber 3 is transparently arranged at the top and bottom of the box body 1; a detection module and an environmental adjustment module connected with the control module are arranged in the control chamber 4, the detection module is used to detect the temperature, humidity and bacterial content information in the culture chamber 3, and transmit the temperature, humidity and bacterial content information to the control module, the control module controls the environmental adjustment module to work according to the temperature and bacterial content information in the culture chamber 3, to ensure that the temperature and bacterial content in the culture chamber 3 are at the set value.

[0036] The power supply is a mobile power supply, which is electrically connected with the control module, the detection module and the environmental adjustment module, and is used to provide working power for the control module, the detection module and the environmental adjustment module. The mobile power supply is a rechargeable power supply.

[0037] The front end and the rear end of the box body 1 are provided with clamping openings 9 for facilitating the clamping of a mechanical hand, and the clamping openings 9 facilitate the clamping of the culture box by a mechanical arm.

[0038] A humidifying groove 16 is arranged along the bottom periphery of the culture chamber 3, and a culture dish placing rack 10 is arranged on the periphery of the inner wall of the culture chamber 3 above the humidifying groove 16. The detection module includes a temperature sensor, a humidity sensor and a gas sensor. When the humidity value in the culture chamber 3 is detected to be less than the set threshold value of the control module, water is manually added to the humidifying groove 16.

[0039] The box body 1 includes a cover plate 5 rotatably arranged at the top of the culture chamber, the cover plate 5 includes a support frame 501 and a glass 502 arranged in the support frame 501; the environmental adjustment module includes a first heating plate 14 and a second heating plate 15, the first heating plate 14 is fixedly installed in the support frame 501 below the glass 502, and the second heating plate 15 is fixedly installed at the bottom of the box body 1 corresponding to the culture chamber 3.

[0040] The air inlet is communicated with the air inlet 7, and the air outlet is arranged on the other side vertical surface in the culture chamber 3. The air inlet and the air outlet are respectively provided with a breathing valve 8. The specific structure and installation mode of the breathing valve 8 are both prior art, and will not be described in detail here. The model is selected to be only breathable and not to pass through liquid, which is common knowledge and will not be described here. The breathing valve 8 includes a mounting frame, and one or more first holes are formed in the mounting frame. The first hole is matched with the air inlet, and a breathable film is arranged in the first hole. A sealing ring is arranged along the circumference of the first hole. A handle is fixedly arranged on one side of the first hole. The top of the air inlet and the air outlet is provided with a second hole matched with the handle.

[0041] The control module includes a touch screen 11 arranged at the top of the box 1 corresponding to the control chamber 4, a communication interface chip U37 and a main control chip arranged in the control chamber 4. The communication interface chip U37 and the main control chip are adaptively connected. The touch screen 11 realizes communication interaction through the communication interface chip U37 and the main control chip. The internal structure and specific connection of the communication interface chip U37 and the main control chip are shown in Figure 10 、 8 , which are prior art and will not be described in detail here.

[0042] The environmental regulation module includes two UVC ultraviolet lamps arranged in the culture chamber 3. The two UVC ultraviolet lamps are both connected with a communication isolation unit. The main control chip controls the opening and closing of the two UVC ultraviolet lamps by controlling the two communication isolation units. The internal structure and specific connection of the two communication isolation units and the main control chip are shown in Figure 9 、 8 , which are prior art and will not be described in detail here.

[0043] The main control chip is adaptively connected with a 485 communication unit one and a 485 communication unit two. The first heating plate 14 and the second heating plate 15 are respectively connected with a temperature control unit one and a temperature control unit two. The port P7 of the temperature control unit one is connected with the 485 communication unit one. The port P14 of the temperature control unit two is connected with the 485 communication unit two. The specific internal principle diagram of the 485 communication unit one and the 485 communication unit two is shown in Figure 11 . The connection of the main control chip and the first heating plate 14 and the second heating plate 15 is prior art and will not be described in detail here.

[0044] A one-key switch 12 and a sterilization switch are arranged on the top of the box 1 on one side of the touch screen 11. The one-key switch 12 is connected with the control module, the detection module and the environmental regulation module. The sterilization switch is connected with the UVC ultraviolet lamp.

[0045] The sterilization button 13 is used to turn on and off the UVC ultraviolet lamp, and the one-key switch 12 is used to control the opening and closing of the touch screen 11, temperature sensor, humidity sensor, gas sensor, UVC ultraviolet lamp, communication chip P7 and communication chip P14.

[0046] The base 2 is also provided for charging the mobile power supply, and the top end of the base 2 is provided with a clamping mechanism 6 and an inflation mechanism. The cultivation chamber 3 is provided with an inflation port 7 corresponding to the bottom of the box body 1. The clamping mechanism is used to realize the connection of the box body 1 and the base 2, so that the inflation port 7 and the inflation mechanism are connected.

[0047] The clamping mechanism 6 includes two limiting cones symmetrically arranged at the top of the base 2, and the bottom of the box body 1 is correspondingly provided with a limiting hole. The inflation mechanism includes a quick connector arranged between the two limiting cones and a rubber ring arranged along the circumference of the quick connector. The quick connector penetrates the base 2 and is provided with a circular groove along the circumference of the inflation port 7. The circular groove and the rubber ring are matched, and the base 2 is provided with a pipeline for inflating the quick connector. The charging mechanism includes a fixed sheet arranged at the top of the base 2, a spring needle arranged at the top of the fixed sheet, and a charging socket arranged between the spring needles. The fixed sheet is arranged on one side of one of the limiting cones. One end of the charging socket is connected to an external power supply, and the other end of the charging socket is used to charge the mobile power supply. The box body 1 on the bottom of the mobile power supply is provided with a charging port corresponding to the position of the charging socket and matched with the charging socket. When charging is needed, the limiting hole is placed on the limiting cone. At this time, the quick connector is connected to the inflation port, and the spring needle is connected to the charging port.

[0048] The base 2 is provided with a gas pipeline for providing gas source for the inflation mechanism.

[0049] Principle: Close the cover plate 5 to make the cultivation chamber 3 in a closed state. Click the one-key switch 12 to start the box body 1. The detection module detects the temperature, humidity and bacterial composition in the cultivation chamber 3 in the running state, and sends the detection data to the control module through the wireless module.

[0050] The data detected by the detection module is displayed to the user through the touch screen 11. The control module compares the detection data with the set value to adjust the temperature and bacterial information in the cultivation chamber 3, and then sends the control instruction to the control module through the touch screen 11. The first heating plate 14 and the second heating plate 15 are controlled to be powered on or off, and the UVC ultraviolet lamp is turned on or off. According to the humidity information detected by the detection module, the user adds water to the humidifying tank 16.

[0051] In the process of normal growth and differentiation of cells, the whole device is placed under the observation device by using the external mechanical arm clamping clamp 9 to clamp the clamping port 9. In addition, because the top and bottom glasses in the device are transparent glass, when it is necessary to observe the cultured cells in the middle, the cell culture dish does not need to be taken out, and can be directly observed through the transparent box 1 top, thereby ensuring that the growth environment of the subsequent cell culture will not change, thereby avoiding the influence of environmental changes on experimental data.

[0052] The single-layer culture dish and the environmental regulation module are arranged to allow the temperature, humidity and gas composition information in the culture chamber 3 to be obtained by the control module, and the environmental regulation module is controlled according to the information, thereby accurately ensuring that all cells on the culture dish are in the same growth environment, thereby ensuring that all cells can grow and differentiate normally, thereby ensuring the reliability of the cell experimental data.

[0053] Other embodiments of the application will be apparent to those skilled in the art from consideration of the specification and practice of the application disclosed herein. It is intended that the specification and examples be considered as exemplary only, with the true scope and spirit of the application being indicated by the following claims.

[0054] It should be understood that the application is not limited to the precise construction that has been described above and illustrated in the accompanying drawings, and that various modifications and changes can be made by those skilled in the art without departing from the scope of the application. The scope of the application is limited only by the claims that follow.

Claims

1. A wireless portable compact cell culture incubator, characterized by: The application relates to a cell culture device, which comprises a box body, a control module, a base and a power supply, a culture cavity for placing cell culture dishes and a control chamber for arranging the control module are arranged in the box body; the culture cavity is transparently arranged at the top and the bottom of the box body; a detection module and an environment adjusting module are arranged in the control chamber and are connected with the control module; the detection module is used for detecting the temperature, humidity and bacterial content information in the culture cavity and transmitting the temperature, humidity and bacterial content information to the control module; the control module controls the environment adjusting module to work according to the temperature and the bacterial content information in the culture cavity, so that the temperature and the bacterial content in the culture cavity are kept at the set values. The power supply is a mobile power supply which is electrically connected with the control module, the detection module and the environment adjusting module and is used for providing working power for the control module, the detection module and the environment adjusting module. The environment adjusting module comprises a first heating plate, a second heating plate and two UVC ultraviolet lamps; the first heating plate is fixedly arranged on a cover plate at the top of the culture cavity in a transparent mode; the second heating plate is fixedly arranged at the bottom of the box body corresponding to the culture cavity in a transparent mode; the two UVC ultraviolet lamps are arranged in the culture cavity; the control module comprises a main control chip; the main control chip is connected with the first heating plate through a 485 communication unit 1 and controls the first heating plate to work or stop through the 485 communication unit 1; the main control chip is connected with the second heating plate through a 485 communication unit 2 and controls the second heating plate to work or stop through the 485 communication unit 2; the two UVC ultraviolet lamps are connected with communication isolation units; the main control chip controls the opening and closing of the two UVC ultraviolet lamps by controlling the two communication isolation units; a humidifying groove is further arranged along the bottom periphery of the culture cavity. A clamping mechanism, an inflation mechanism and a charging mechanism are arranged at the top of the base; the clamping mechanism comprises two limiting cones which are symmetrically arranged at the top of the base; the inflation mechanism comprises a quick connector which penetrates through the base and a pipeline arranged in the base and used for inflating the quick connector; the charging mechanism comprises a fixed sheet arranged at the top of the base, spring pins arranged at the top of the fixed sheet and charging jacks arranged between the spring pins in a spaced mode; a charging port corresponding to the charging jacks and matched with the charging jacks is arranged on the box body at the bottom of the mobile power supply; the bottom of the box body corresponding to the culture cavity is provided with an inflation port, a charging port and a limiting hole; when the box body is connected with the base, the limiting hole is placed on the limiting cone, the quick connector is connected with the inflation port and the spring pins are connected with the charging port.

2. The wireless portable compact cell culture incubator according to claim 1, wherein, The cover plate is rotatably arranged at the top of the culture cavity; the cover plate comprises a support frame and a glass I arranged in the support frame; the first heating plate is fixedly arranged in the support frame below the glass I in a transparent mode.

3. A wireless portable compact cell culture incubator according to claim 2, wherein, An air inlet communicating with the inflation port is arranged on one side vertical surface of the culture cavity; an air outlet is arranged on the other side vertical surface of the culture cavity; a breathing valve is arranged on the air inlet and the air outlet respectively.

4. The wireless portable compact cell culture incubator according to claim 1, wherein, Clamping ports facilitating the clamping of a mechanical hand are arranged at the front end and the rear end of the box body.

5. The wireless portable compact cell culture incubator of claim 1, wherein, The inner wall periphery of the culture cavity above the moisturizing tank is provided with a culture dish placing rack.

6. The wireless portable compact cell culture incubator of claim 1, wherein, The control module comprises a touch screen arranged at the top of the corresponding box in the control chamber, and a communication interface chip arranged in the control chamber; The communication interface chip and the main control chip are adaptively connected, and the touch screen realizes communication interaction through the communication interface chip and the main control chip.

7. A wireless portable compact cell culture incubator according to claim 6, wherein, A one-key switch and a sterilization switch are arranged at the top of the box on one side of the touch screen, the one-key switch is connected with the control module, the detection module and the environment adjusting module, and the sterilization switch is connected with the UVC ultraviolet lamp.

8. The wireless portable compact cell culture incubator according to claim 2, wherein, The base is internally provided with a gas path pipeline for providing a gas source for the inflation mechanism.

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