A method for determining the bioavailability of antibiotics in soil using continuous solvent extraction-solid phase extraction-liquid chromatography-tandem mass spectrometry.

By employing a continuous solvent extraction-solid phase extraction-liquid chromatography-tandem mass spectrometry method and optimizing pretreatment parameters, the sensitivity and accuracy issues in detecting the bioavailability of antibiotics in soil were resolved. This method enables the detection of trace antibiotics with high sensitivity and low detection limit, and is suitable for soil antibiotic analysis under complex matrix conditions.

CN118604199BActive Publication Date: 2025-12-02ZHEJIANG UNIV
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Patent Information

Application Number
CN202410891784.5
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2024-07-04
Publication Date
2025-12-02
Estimated Expiration
2044-07-04

AI Technical Summary

Technical Problem

Existing technologies have low sensitivity and limited accuracy in detecting the bioavailability of antibiotics in soil, and the complex matrix causes significant interference, resulting in significant deviations in antibiotic concentration measurements and making it impossible to accurately assess environmental risks.

Method used

A continuous solvent extraction-solid phase extraction-liquid chromatography-tandem mass spectrometry method was adopted. The antibiotic components were enriched by 0.01M calcium chloride extract and HLB column, combined with vacuum centrifugation concentration. The pretreatment parameters were optimized, and multiple reaction monitoring was used to quantify the antibiotic components, reduce matrix interference, and improve detection sensitivity and accuracy.

Benefits of technology

It enables the analysis and detection of trace antibiotic bioactive components and extractable components with high sensitivity and low detection limit under complex matrix conditions. It is suitable for the accurate detection of trace antibiotics in soil, reducing the detection limit and matrix interference.

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Abstract

This invention discloses a method for detecting the bioavailability of antibiotics in soil using continuous solvent extraction-solid phase extraction-liquid chromatography-tandem mass spectrometry. The method first uses continuous solvent extraction to extract the bioactive and extractable antibiotic components from the soil. Then, a solid phase extraction column is used for sample purification. Finally, multiple reaction monitoring (MRM) mode of liquid chromatography-tandem mass spectrometry is used to determine the concentrations of various antibiotics, including chlortetracycline, doxycycline, oxytetracycline, tetracycline, sulfadiazine, sulfadiazine, sulfamethoxazole, ciprofloxacin, enrofloxacin, norfloxacin, ofloxacin, azithromycin, erythromycin, roxithromycin, tylosin, trimethoprim, and lincomycin. This invention combines continuous solvent extraction with solid phase extraction, coupled with liquid chromatography-tandem mass spectrometry detection, to achieve accurate evaluation of trace antibiotic bioavailability under complex matrix conditions.
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