A polysaccharide composition with moisturizing and anti-allergic soothing effects, and its preparation method and application

Abstract

The present invention relates to a polysaccharide composition with moisturizing and anti-allergic soothing effects, and a preparation method and application thereof. The polysaccharide composition comprises hydrolyzed corn starch with a straight chain linear structure of a polysaccharide polymerization degree of 2-20. By optimizing the type of hydrolyzed corn starch and compounding other multiple polysaccharides, a polysaccharide composition with significant moisturizing and anti-allergic soothing effects is provided, which provides a new anti-allergic soothing approach for the development of cosmetic products.

Description

Technical Field

[0001] The present invention relates to the field of cosmetics, and in particular to a polysaccharide composition with moisturizing, anti-allergic and soothing effects, and a preparation method and application thereof. Background Art

[0002] With the development of the economy, the amount of harmful substances in the environment to the skin has also increased accordingly. In addition, due to the increase in life pressure, skin sensitivity caused by external and internal factors has increased significantly in recent years, and the demand for anti-allergic skin care products has also increased.

[0003] As a highly active substance in nature, natural polysaccharides have multiple beauty activities such as moisturizing, whitening, anti-aging, and skin repair, and have become a research and development hotspot in the industry.

[0004] Hydrolyzed corn starch exists in natural plants and can be used in cosmetics as an anti-itching ingredient for the scalp or body skin. It can be added to shampoo, shower gel or other personal care products to promote good anti-itching and moisturizing effects on the skin.

[0005] The present application provides a polysaccharide composition with significant moisturizing and anti-allergic soothing effects by optimizing the type of hydrolyzed corn starch and compounding other multiple polysaccharides. Summary of the invention

[0006] Based on this, in order to solve at least one technical problem existing in the prior art, the present invention generally provides a polysaccharide composition with moisturizing and anti-allergic and soothing effects, and a preparation method and application thereof.

[0007] The first aspect of the present invention provides a polysaccharide composition with moisturizing and anti-allergic soothing effects; comprising hydrolyzed corn starch, wherein the hydrolyzed corn starch is a straight-chain linear structure, and the degree of polymerization of the polysaccharide is 2-20, and while having a moisturizing effect, it also has a good anti-allergic soothing effect. Preferably, the degree of polymerization of the polysaccharide of the hydrolyzed corn starch is 3-10.

[0008] Preferably, the polysaccharide composition further comprises one or more of maltose, oligofructose, rhamnose and inulin.

[0009] Preferably, the polysaccharide composition comprises five polysaccharides, namely, hydrolyzed corn starch, maltose, oligofructose, rhamnose and inulin.

[0010] These polysaccharide component substances complement each other. The large molecular polysaccharides form a crystal film on the surface of the skin. The polyhydroxy molecular structure locks in skin moisture very well and has an excellent moisturizing effect. At the same time, the barrier formed by the crystal film effectively resists external stimuli. The preferred small molecular polysaccharides such as hydrolyzed corn starch penetrate into the skin granular layer, which can effectively maintain the loss of internal skin moisture and inhibit the formation of inflammatory factors, thereby reducing irritation. It has a synergistic effect on the long-lasting moisturizing and soothing and anti-inflammatory effects of skin care products, and can realize the long-lasting moisturizing and soothing and anti-inflammatory functions of skin care products.

[0011] Preferably, the composition comprises, by weight percentage, 1-50% hydrolyzed corn starch, 0.1-20% maltose, 0-30% fructooligosaccharide, 0-20% rhamnose, and 0.1-30% inulin.

[0012] Preferably, the composition comprises, by mass percentage, 1-30% hydrolyzed corn starch, 0.1-10% maltose, 0.1-10% oligofructose, 0.1-10% rhamnose, and 0.1-10% inulin.

[0013] Furthermore, it comprises 10-20% hydrolyzed corn starch, 5-10% maltose, 5-10% oligofructose, 1-10% rhamnose and 1-10% inulin.

[0014] Preferably, the polysaccharide composition further comprises a balance of preservatives and solvents.

[0015] Preferably, the preservative is one or a combination of 1,2-hexanediol, 1,2-pentanediol, capryl ketone, caprylhydroxamic acid, phenoxyethanol, ethylhexylglycerin, caprylyl glycol, and sodium benzoate.

[0016] Preferably, the solvent is one or a combination of water, maltitol, erythritol, butylene glycol, propylene glycol, glycerin, yeast fermentation broth, plant extract floral water, and aloe vera water.

[0017] The present invention also provides a method for preparing the above-mentioned polysaccharide composition having moisturizing and anti-allergic and soothing effects, comprising the following steps:

[0018] The solvent is sterilized in advance and heated to 50°C. Hydrolyzed corn starch, maltose, oligofructose, rhamnose and inulin are added at the same time. The solvent is stirred until it is completely dispersed. The preservative is added and the stirring is continued until the solvent is evenly dispersed.

[0019] The present invention also provides the use of the polysaccharide composition in cosmetics, which can be applied to different types of cosmetics, including but not limited to face cream, eye cream, facial cleanser, shower gel, shampoo, essence cream, toner, lotion, facial mask and other products.

[0020] For example, in one of the embodiments, a cosmetic essence comprises, by weight percentage, 5% of the polysaccharide composition, 0.2% of Carbomer U2020, 2% of PEG / PPG-17 / 6 copolymer, 5% of 1,3-butylene glycol, 0.3% of vitamin C ethyl ether, 0.2% of 4-butylresorcinol, 0.5% of 1,2-hexanediol, 0.5% of p-hydroxyacetophenone, and the remainder of water.

[0021] Compared with the prior art, the present invention has the following beneficial effects:

[0022] The polysaccharide composition of the present invention is preferably of the hydrolyzed corn starch type, and is compounded with other multiple polysaccharides to provide a polysaccharide composition with significant moisturizing and anti-allergic soothing effects, wherein the polysaccharide component substances complement each other, and the large molecular weight polysaccharide forms a crystal film on the surface of the skin, and the molecular structure of the polyhydroxy group locks the moisture of the skin well, which has an excellent moisturizing effect. At the same time, the barrier formed by the crystal film effectively resists external stimuli, and the preferred small molecular weight polysaccharides such as hydrolyzed corn starch penetrate into the granular layer of the skin, which can well maintain the loss of moisture inside the skin, and can inhibit the generation of inflammatory factors, thereby reducing irritation, and has a synergistic effect on the long-lasting moisturizing and soothing and anti-inflammatory effects of skin care products, and can achieve the long-lasting moisturizing and soothing and anti-inflammatory functions of skin care products. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 This is a picture of the chicken embryo chorioallantoic membrane test;

[0024] Figure 2 This is a picture of the zebrafish inflammatory cell inhibition rate test. DETAILED DESCRIPTION

[0025] In order to make the purpose, technical scheme and advantages of the embodiments of the present invention clearer, the technical scheme in the embodiments of the present invention will be clearly and completely described below. The above-mentioned polysaccharide composition will be used as an effective component to prepare a moisturizing and soothing essence, and the present invention will be further explained in combination with specific implementation methods.

[0026] The polysaccharide composition of the present invention can be added as a functional additive to cosmetics of different dosage forms such as water solution, emulsion, cream, essence, facial mask, facial cleanser, etc. Obviously, the described embodiments are part of the embodiments of the present invention, but not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0027] Unless otherwise specified, the sources of reagents used in the following examples and comparative examples are all commercially available; the methods used in the following examples and comparative examples, unless otherwise specified, are all achievable by conventional methods.

[0028] Example 1

[0029] A polysaccharide composition, comprising, by weight percentage: 20% hydrolyzed corn starch, 5% maltose, 10% oligofructose, 8% rhamnose, 10% inulin, 2% preservative, and 45% solvent;

[0030] Wherein, the degree of polymerization of the polysaccharide of the hydrolyzed corn starch is within the range of 2-20, and all of them are straight-chain linear molecular structures;

[0031] Wherein, the preservative is 1,2-hexanediol;

[0032] Wherein, the solvent is deionized water.

[0033] The preparation method of the polysaccharide composition is as follows:

[0034] Sterilize deionized water in advance, heat it to 50°C, add hydrolyzed corn starch, maltose, oligofructose, rhamnose, and inulin, stir to dissolve and disperse completely, add 1,2-hexanediol, and continue to stir to disperse evenly.

[0035] Example 2

[0036] A polysaccharide composition, which is different from Example 1 in that it comprises: 10% hydrolyzed corn starch, 5% maltose, 5% oligofructose, 8% rhamnose, 5% inulin, 2% preservative, and 65% solvent.

[0037] Example 3

[0038] A polysaccharide composition, which is different from Example 1 in that the degree of polymerization of the polysaccharide of the hydrolyzed corn starch is within the range of 3-10.

[0039] Comparative Example 1

[0040] A polysaccharide composition, which is different from Example 1 in that it comprises: 5% maltose, 10% oligofructose, 8% rhamnose, 10% inulin, 2% preservative, and 65% solvent

[0041] Comparative Example 2

[0042] A polysaccharide composition, which is different from Example 1 in that it comprises: 20% hydrolyzed corn starch, 10% oligofructose, 8% rhamnose, 10% inulin, 2% preservative, and 50% solvent

[0043] Comparative Example 3

[0044] A polysaccharide composition, which is different from Example 1 in that it comprises: 20% hydrolyzed corn starch, 5% maltose, 8% rhamnose, 10% inulin, 2% preservative, and 55% solvent

[0045] Comparative Example 4

[0046] A polysaccharide composition, comprising: 20% hydrolyzed corn starch, 5% maltose, 10% oligofructose, 10% inulin, 2% preservative, and 53% solvent

[0047] Comparative Example 5

[0048] A polysaccharide composition comprises: 20% hydrolyzed corn starch, 5% maltose, 10% oligofructose, 8% rhamnose, 2% preservative and 55% solvent.

[0049] Comparative Example 6

[0050] A polysaccharide composition, which is different from Example 1 in that the degree of polymerization of the polysaccharide in the hydrolyzed corn starch is within the range of 30-50.

[0051] The formula table of each embodiment and comparative example is shown in Table 1.

[0052] Table 1: Examples and Comparative Examples

[0053]

[0054] Application Example 1

[0055] A cosmetic essence comprises: 5% of a polysaccharide composition, 0.2% of Carbomer U2020, 2% of a PEG / PPG-17 / 6 copolymer, 5% of 1,3-butylene glycol, 0.3% of vitamin C ethyl ether, 0.2% of 4-butylresorcinol, 0.5% of 1,2-hexanediol, 0.5% of p-hydroxyacetophenone, and the balance of water.

[0056] Wherein, the polysaccharide composition is the polysaccharide composition in Example 1;

[0057] The preparation method of the cosmetic essence comprises the following steps:

[0058] Soak and disperse Carbopol U2020 in advance, mix the soaked U2020 with deionized water, 1,3-butylene glycol, and vitamin C ethyl ether, stir and disperse them evenly, add PEG / PPG-17 / 6 copolymer, 4-butylresorcinol, 1,2-hexanediol, and p-hydroxyacetophenone, and continue stirring until they are completely dissolved.

[0059] Application Example 2

[0060] A cosmetic essence, which is different from Application Example 1 in that the polysaccharide composition in Example 2 is used;

[0061] Application Example 3

[0062] A cosmetic essence, which is different from Application Example 1 in that the polysaccharide composition in Example 3 is used;

[0063] Comparative application example 1

[0064] A cosmetic essence, which is different from Application Example 1 in that the polysaccharide composition in Comparative Example 1 is used;

[0065] Comparative Application Example 2

[0066] A cosmetic essence, which is different from Application Example 1 in that the polysaccharide composition in Comparative Example 2 is used;

[0067] Comparative Application Example 3

[0068] A cosmetic essence, which is different from Application Example 1 in that the polysaccharide composition in Comparative Example 3 is used;

[0069] Comparative Application Example 4

[0070] A cosmetic essence, which is different from Application Example 1 in that the polysaccharide composition in Comparative Example 4 is used;

[0071] Comparative Application Example 5

[0072] A cosmetic essence, which is different from Application Example 1 in that the polysaccharide composition in Comparative Example 5 is used.

[0073] Comparative Application Example 6

[0074] A cosmetic essence, which is different from Application Example 1 in that the polysaccharide composition in Comparative Example 6 is used.

[0075] Test Section

[0076] 1) Chicken embryo chorioallantoic membrane test (soothing and irritation reduction test)

[0077] Basic principle of the test: The chorioallantoic membrane (CAM) is a respiratory membrane that surrounds the chicken embryo. Since the surface of the chicken embryo chorioallantoic membrane is rich in blood vessels and can be regarded as a complete organism, this test uses the characteristics of the complete, clear and transparent vascular system of the chorioallantoic membrane of the mid-stage incubated chicken embryo. A certain amount of the test substance is directly exposed to the chicken embryo chorioallantoic membrane. After the specified time, the changes in the toxic effect indicators of the chorioallantoic membrane (such as bleeding, coagulation and vascular melting) are observed, and scores are given. The mathematical mean value is calculated to evaluate the eye irritation of the test substance.

[0078] Test method and process:

[0079] Observe the test items and calculate the scores.

[0080] In the formal test, 6 chicken embryos are used for each test substance. 0.3 ml or 0.3 g of the sample is aspirated or weighed onto the chorioallantoic membrane, covering the surface as much as possible. After exposure for 5 minutes (if the sample is solid, after contact for 3 minutes, gently rinse with physiological saline and pour out), the degree of change of each toxic effect is immediately observed under a stereomicroscope, and the time or degree of each bleeding, coagulation, and vascular melting from the start of the test is observed and recorded, and the irritation score is performed using the time evaluation method and the endpoint scoring method.

[0081] In addition, the scoring range is set: 0-6 (including 6) is no irritation or mild irritation, 6 (excluding 6)-12 (including 12) is moderate irritation, and above 12 is severe irritation.

[0082] The test results are shown in Table 2. Figure 1 Test pictures of some application examples and comparative application examples are given in the figure.

[0083] Table 2 Test results of soothing and irritation reduction test

[0084]

[0085] From the conclusion of Table 1 above, it can be seen that the polysaccharide composition containing five polysaccharide combinations in the embodiment of the present invention has significantly lower irritation than the control group, and reducing any one of them has a greater impact on irritation, especially hydrolyzed corn starch, followed by oligofructose, maltose, rhamnose, inulin, etc. The polysaccharide composition of the embodiment of the present invention is applied to essence products to obtain products with lower irritation.

[0086] 2) Zebrafish inflammatory cell inhibition rate test (anti-allergy and anti-irritation test)

[0087] Test method:

[0088] Zebrafish embryo inflammation model: Zebrafish embryos were exposed to CuSO4 to create an inflammation model.

[0089] The inhibition rate of zebrafish embryo inflammatory cells (neutrophils) = [(the number of inflammatory cells in the zebrafish embryo inflammation model control group - the number of inflammatory cells in the zebrafish embryo inflammation model sample treatment group) × 100 / the number of inflammatory cells in the zebrafish embryo inflammation model control group]%.

[0090] The p value is obtained by T-Test analysis of the number of inflammatory cells in the zebrafish embryo inflammation model sample treatment group and the number of inflammatory cells in the zebrafish embryo inflammation model control group. Generally, p<0.05 is considered statistically significant. Based on the inhibition rate of the sample on zebrafish embryo inflammatory cells and the statistically significant difference in the inhibition rate, the soothing effect of the sample is evaluated as significant (the inhibition rate is positive and p<0.05) and insignificant (the inhibition rate is negative or p≥0.05).

[0091] The test results are shown in Table 3. Figure 2 2 shows the comparison of test pictures before and after the test of Application Example 1.

[0092] Table 3 Zebrafish inflammatory cell inhibition rate test results

[0093]

[0094] From the conclusion of Table 3 above, it can be seen that the polysaccharide composition containing five polysaccharide combinations in the embodiment of the present invention has a good inhibitory effect on inflammatory cells, especially the hydrolyzed corn starch has a greater contribution. It shows that the hydrolyzed corn starch with a linear molecular structure of a polysaccharide polymerization degree of 2-20 selected in the embodiment of the present invention has an excellent anti-inflammatory effect and excellent anti-allergic and anti-irritation properties. The polysaccharide composition of the embodiment of the present invention is used in essence products, which has a significant inhibitory effect on inflammatory cells.

[0095] 3) Moisturizing data test

[0096] The essences of Application Examples 1 to 3, Comparative Application Examples 1 to 6 and commercially available common essences were used by 180 subjects, all of whom were female and aged between 18 and 50 years old. The subjects were divided into 9 groups, each with 20 subjects, and each group of subjects used the same facial mask. The subjects were placed in an indoor environment with a temperature of 20°C and a relative humidity of 50%, and a fixed area of ​​2 cm2 was drawn on the forehead. After using the facial mask on the fixed area, the skin moisture detector was used to test the skin moisture change data, and the water replenishment rate and moisturizing rate were calculated.

[0097] Moisture replenishment rate (%) = (humidity value after use - humidity value before use) / humidity value before use × 100%,

[0098] Moisture retention rate (%) = humidity value after 1 hour of use / humidity value after use × 100%,

[0099] The humidity values ​​after use, before use, and after 1 hour of use are the average values ​​of each group of subjects.

[0100] The test results are shown in Table 4:

[0101] Table 4 Moisturizing test results

[0102] Group Humidity value before use Humidity value after use Humidity value after 1h Water replenishment rate (% Moisturizing rate (%) Application Example 1 32.5 65.5 58.8 101.5 89.8 Application Example 2 32.5 45.5 37.6 40.0 82.6 Application Example 3 32.3 62.5 57.5 78.0 92.0 Comparative application example 1 32.0 48.8 36.2 52.5 74.2 Comparative Application Example 2 32.8 55.7 43.6 69.9 78.3 Comparative Application Example 3 31.9 58.5 44.7 83.4 76.4 Comparative Application Example 4 31.5 59.2 43.4 87.9 73.3 Comparative Application Example 5 32.2 53.0 42.5 64.6 80.2 Comparative Application Example 6 31.5 66.5 60.6 111.1 91.1

[0103] From the conclusion of Table 4 above, it can be seen that the polysaccharide composition containing five polysaccharide combinations according to the embodiment of the present invention has excellent moisturizing effect, and the moisturizing rate is significantly reduced when any one of the polysaccharides is reduced. The polysaccharide composition according to the embodiment of the present invention is applied to essence products to provide excellent moisturizing performance.

[0104] In summary, it can be seen that the hydrolyzed corn starch with a polysaccharide degree of polymerization within the range of 2-20 and a straight-chain linear molecular structure contributes the most to the moisturizing and anti-soothing properties of the polysaccharide composition. By increasing the degree of polymerization of hydrolyzed corn starch, such as in Comparative Application Example 6, a straight-chain hydrolyzed starch with a polysaccharide degree of polymerization of 30-50 is used. Although the moisturizing effect is good, the anti-soothing property is poor. The embodiments of the present invention discover that the hydrolyzed corn starch with a straight-chain linear structure with a suitable polysaccharide degree of polymerization has a moisturizing effect while also having a good anti-allergic and soothing effect; at the same time, compounding with other polysaccharides can further improve its irritation properties. The polysaccharide composition of the embodiments of the present invention broadens the application scope of hydrolyzed corn starch and provides a new anti-allergic and soothing approach for the development of cosmetic products.

[0105] The technical features of the above-described embodiments may be arbitrarily combined. To make the description concise, not all possible combinations of the technical features in the above-described embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0106] The above-mentioned embodiments only express several implementation methods of the present invention, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the invention patent. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

Claims

1. A polysaccharide composition having moisturizing and anti-allergic soothing effects, characterized in that: According to the mass percentage of the composition, it includes 1-30% hydrolyzed corn starch, 0.1-10% maltose, 0.1-10% oligofructose, 0.1-10% rhamnose, and 0.1-10% inulin. The hydrolyzed corn starch is a straight-chain linear structure, and the polymerization degree of the polysaccharide is 2-20.

2. The polysaccharide composition according to claim 1, characterized in that: The hydrolyzed corn starch is a straight-chain linear structure, and the degree of polymerization of the polysaccharide is 3-10.

3. The polysaccharide composition according to claim 1 or 2, characterized in that: Balance of preservatives and solvents are also included.

4. The polysaccharide composition according to claim 3, characterized in that: The preservative is one or a combination of 1,2-hexanediol, 1,2-pentanediol, capryl ketone, caprylhydroxamic acid, phenoxyethanol, ethylhexylglycerin, caprylyl glycol, and sodium benzoate.

5. The polysaccharide composition according to claim 3, characterized in that: The solvent is one or a combination of water, maltitol, erythritol, butylene glycol, propylene glycol, glycerin, yeast fermentation liquid, plant extract flower water, and aloe water.

6. The method for preparing a polysaccharide composition according to any one of claims 1 to 5, characterized in that: The following steps are involved: The solvent is sterilized in advance, heated to 50°C, and the formulated amount of hydrolyzed corn starch, maltose, oligofructose, rhamnose, and inulin are added at the same time, stirred and dispersed completely, and the preservative is added, and the stirring is continued to disperse evenly.

7. A cosmetic comprising the polysaccharide composition according to any one of claims 1 to 6.

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