A moisturizing composition, its preparation method and application

Abstract

The present invention discloses a moisturizing composition, a preparation method and an application thereof. The composition comprises the following components in parts by weight: 20-40 parts of plant extract, 10-25 parts of ceramide, 5-15 parts of algooligosaccharides, 1-8 parts of filtrate of fermentation product of Saccharomycopsis galactica, 0.5-2 parts of pyrrolidone carboxylic acid and 35-60 parts of polyol; the plant extract is an extract of ginkgo leaf, cornflower, centella asiatica, rosa laevigata and aloe vera. The present invention prepares a moisturizing composition from the above components, and good synergistic effects are generated among the raw materials, which can quickly supply water in a short term. Long-term use can repair and enhance the skin barrier, improve the water-locking ability of the skin, increase the water content in the skin, and achieve long-term moisturization.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and particularly relates to a moisturizing composition, a preparation method and an application thereof. Background Art

[0002] Moisturizing is an important part of daily skin care products for people, which is used to maintain the moisture of the stratum corneum, maintain skin elasticity and softness, and maintain the skin barrier function. After the skin moisture increases, it can accelerate metabolism, discharge blockages, reduce melanin, and is beneficial to fade freckles. Moisturizing can improve the dull skin texture, increase brightness, and make the skin texture smooth and delicate.

[0003] There are already many moisturizing products for moisturizing on the market. Most of them have a short-lasting moisturizing effect, and the long-lasting moisturizing function is not significant, and they cannot effectively enhance the skin barrier function. Therefore, it is necessary to develop a composition that can achieve rapid moisturizing, long-lasting moisturization, and can significantly enhance the skin barrier function. Summary of the Invention

[0004] In view of the above problems, the purpose of the present invention is to provide a moisturizing composition, a preparation method and an application thereof, which can rapidly moisturize in a short time. Long-term use can not only increase the water content in the skin, but also repair and enhance the skin barrier, improve the water-locking ability of the skin, and achieve long-lasting moisturization.

[0005] In the first aspect, the present invention provides a moisturizing composition, which comprises the following components in parts by weight: 20-40 parts of plant extract, 10-25 parts of ceramide, 5-15 parts of algooligosaccharides, 1-8 parts of filtrate of Saccharomyces cerevisiae ferment filtrate, 0.5-2 parts of pyrrolidone carboxylic acid, and 35-60 parts of polyol; the plant extract is an extract of Sedum rubrotinctum, Centaurea cyanus, Centella asiatica, Rosa laevigata and Aloe vera.

[0006] Further, the mass ratio of Sedum rubrotinctum, Centaurea cyanus, Centella asiatica, Rosa laevigata and Aloe vera is (1-2):(1-2):(1-2):(1-2):(2-3), and the active ingredients are extracted after mixing according to this ratio.

[0007] Further, the ceramide is a composition, which is composed of ceramide 1, ceramide AP and ceramide 6II, and the mass ratio of ceramide 1, ceramide AP and ceramide 6II is (1-3):(2-5):(0.05-1).

[0008] Further, the polyol is at least one of glycerol, butanediol, dipropylene glycol, sorbitol, 1,2-pentanediol, 1,2-hexanediol, erythritol, glycerol polyether-26, and octyldecanol.

[0009] Further, the preparation method of the plant extract comprises the following steps:

[0010] S1, pulverize succulent red vegetables, cornflowers, asiatic pennywort herb, fructus rosae laevigatae, and aloe vera into powder with a pulverizer, add 1,2 - hexanediol and water and mix, soak in hot water at 60 - 80°C for 4 - 8 h, and cool to 30 - 35°C to obtain a mixed raw material;

[0011] S2, add cellulase to the mixed raw material and perform enzymatic hydrolysis for 10 - 20 min;

[0012] S3, heat the raw material processed in S2 to 80 - 95°C, reflux and extract for 3 - 6 h, cool to room temperature, filter, and collect the filtrate;

[0013] S4, concentrate the filtrate obtained in S3 to 1 / 4 - 1 / 2 of the original volume, then add petroleum ether with a volume ratio of 1:1, ultrasonicate for 5 - 10 min, then let stand for 10 - 30 min, separate the organic phase and the aqueous phase, repeat the extraction of the obtained aqueous phase with 1:1 petroleum ether 1 - 2 times, collect the aqueous phase, evaporate, concentrate, and dry to obtain the plant extract.

[0014] Further, the addition amount of cellulase in S2 is 0.1 - 1.5‰ of the mixed raw material.

[0015] Further, the drying method in S4 is freeze - drying, the temperature of freeze - drying is 60 - 70°C, and the time is 3 - 8 h to prepare freeze - dried powder.

[0016] As a preferred technical solution, the water - replenishing and moisturizing composition provided by the present invention further comprises 0.5 - 5 parts of tremella polysaccharide.

[0017] As a preferred technical solution, the water - replenishing and moisturizing composition provided by the present invention further comprises 0.1 - 4 parts of phytosphingosine.

[0018] In a second aspect, the present invention further provides a preparation method of a water - replenishing and moisturizing composition, comprising the following steps:

[0019] S1, mix algooligosaccharide and polyol, heat to 50 - 70°C, homogenize, keep warm to defoam, and cool to 40 - 50°C;

[0020] S2, sequentially add ceramide, filtrate of galactomyces ferment filtrate, pyrrolidone carboxylic acid, and plant extract, and stir and mix evenly to obtain the water - replenishing and moisturizing composition.

[0021] In a third aspect, the present invention further provides the application of the water - replenishing and moisturizing composition described in the first aspect or the water - replenishing and moisturizing composition prepared by the preparation method described in the second aspect in cosmetics, and the cosmetics include lotion, emulsion, cream, facial mask liquid, or essence.

[0022] In the technical solution of the present invention:

[0023] The plant extract contains a variety of active molecules extracted from succulent red vegetables, cornflowers, gotu kola, Rosa laevigata Michx., and aloe vera. By adopting a suitable extraction method, a variety of active molecules are obtained, and these active molecules can exert a variety of skin care effects.

[0024] Cornflower contains rich nutrients such as polysaccharides, flavonoids, bitter glycosides, and vitamins, and has various effects, such as whitening, antioxidant, anti-inflammatory, acne removal, and moisturizing.

[0025] The extract of gotu kola contains a large amount of triterpenoid compounds, such as asiaticoside, madecassoside, asiatic acid, and madecassic acid, etc.

[0026] The fruit of Rosa laevigata Michx. contains citric acid, malic acid, tannins, resins, vitamin C, 17.12% saponins, and also contains rich carbohydrates.

[0027] Aloe vera contains polysaccharides, anthraquinone compounds, proteins, vitamins, and minerals. The anthraquinone compounds in aloe vera have the effects of astringing, softening, moisturizing, and anti-inflammatory on the skin. Anthraquinone compounds: including aloin, aloe-emodin, aloe-chrysophanol, aloesaponarin, alon, aloesin, alomycin, homonataloin, etc., dozens of them.

[0028] Ceramide: It is an important component of the intercellular substance of stratum corneum cells. Ceramide can well repair the stratum corneum and enhance the skin's own defense ability. At the same time, the deep water-locking effect of ceramide is also very strong, which can help the skin replenish water and prevent skin diseases such as xeroderma.

[0029] Algooligosaccharides are a kind of polysaccharides extracted from seaweed, and have various biological activities, including moisturizing, antioxidant, anti-wrinkle, and sunscreen, etc. Oligomeric algooligosaccharides can form a moisturizing film to balance the skin moisture and make the skin softer and smoother.

[0030] The filtrate of the fermentation product of Saccharomyces cerevisiae var. boulardii has bioactive substances with various effects, and has various effects such as antioxidant, anti-inflammatory, antibacterial, and immune regulation, etc., which can improve the skin condition, delay skin aging, and enhance skin immunity.

[0031] Pyrrolidone carboxylic acid is one of the important components of the natural moisturizing factor of the human body, which can make the skin and hair have wettability, softness, elasticity, gloss, and antistatic property.

[0032] Polyols: They have hygroscopicity and can absorb moisture from the outside and supplement it to the epidermal layer of the skin.

[0033] Tremella polysaccharide: Tremella polysaccharide can maintain the moisture in the dermal connective tissue, making the connective tissue in a loose state. As a result, the dermis can always be in a state of sufficient moisture. Even when the moisture in the epidermis is completely depleted, the dermis can quickly transport moisture to the epidermis, preventing the moisture in the keratinocytes from being overly insufficient.

[0034] Phytosphingosine, also known as sphingosine, with the scientific name 2-amino-4-octadecene-1,3-diol, is an 18-carbon amino alcohol containing an unsaturated hydrocarbon chain. It belongs to sphingolipids, is one of the components of cell membranes, and is also a signaling lipid molecule involved in various cellular processes. Phytosphingosine is mainly distributed in plant seeds such as wheat. Phytosphingosine is based on the long-chain sphingomyelin that can serve as the backbone of ceramide and exists in the epidermis and the skin surface. Its substance structure is similar to that of the stratum corneum, and it can quickly penetrate into the skin and combine with the water in the stratum corneum to form a network structure to lock in moisture.

[0035] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0036] The moisturizing composition provided by the present invention uses the mutual cooperation of plant extracts, ceramide, algooligosaccharide, filtrate of Saccharomyces cerevisiae fermentation product, pyrrolidone carboxylic acid, and polyols to enable active molecules to better penetrate into the skin. On the one hand, it promotes the skin to absorb more moisture from the outside, and on the other hand, the active molecules entering the skin form a network structure in the stratum corneum to lock in moisture, thus achieving the effects of rapid water replenishment and long-term moisturization. The plant extracts provided by the present invention are enriched with various active biological molecules from Sedum rubrotinctum, Centaurea cyanus, Centella asiatica, Rosa laevigata, and Aloe vera, and these molecules have various effects to improve the skin. The mutual cooperation of plant extracts, filtrate of Saccharomyces cerevisiae fermentation product, and pyrrolidone carboxylic acid enables more moisture and active molecules to be retained in the keratinocytes, plump the skin, activate cell activity, and improve the skin condition; the mutual cooperation of water-soluble ceramide, algooligosaccharides, phytosphingosine, and polyols forms a film and a network to play a water-locking function to slow down the outward volatilization of moisture and strengthen the skin barrier; the use of Tremella polysaccharide makes the moisture in the dermis in a sufficient state, enabling the dermis to also transport moisture to the epidermis. Therefore, the inventors of this application have found through a large amount of research that using plant extracts, ceramide, algooligosaccharide, filtrate of Saccharomyces cerevisiae fermentation product, polyols, phytosphingosine, and Tremella polysaccharide, etc. as raw materials to be compounded to prepare a moisturizing composition, good synergistic effects are generated among the raw materials, achieving rapid water replenishment. Long-term use can not only increase the water content in the skin, but also repair and enhance the skin barrier, with a long-term moisturizing effect. Detailed implementation manners

[0037] The specific implementation methods of the technical solution of this specification are further described below through specific examples. These examples are for the purpose of describing the technical solution in detail, rather than for limiting the technical solution. Based on the examples in this specification, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of this specification.

[0038] The raw materials used in the present invention are purchased from commercial channels. The experimental methods in the examples of the present invention without specifying specific conditions are usually carried out under conventional conditions or under conditions recommended by the manufacturer. Unless otherwise specified, percentages and parts are calculated by weight.

[0039] Example 1

[0040] A hydrating and moisturizing composition, comprising the following components by weight: 30 parts of plant extract, 15 parts of ceramide, 10 parts of algal oligosaccharides, 1 part of pyrrolidone carboxylic acid, 4 parts of galactosyl yeast fermentation product filtrate and 50 parts of polyols; the plant extract is an extract of red cabbage, cornflower flower, Centella asiatica, Rosa laevigata and aloe vera; the ceramide is a composition consisting of ceramide 1, ceramide AP and ceramide 6II; the mass ratio of ceramide 1, ceramide AP and ceramide 6II is 1:3:0.5; the polyols are glycerol and butylene glycol.

[0041] The preparation method of the plant extract comprises the following steps:

[0042] S1, crushing red cabbage, cornflower, Centella asiatica, Rosa laevigata, and aloe into powder using a grinder, mixing them in a mass ratio of 1:1:1:1:1, adding 1,2-hexanediol and water, soaking them in 65° C. hot water for 5 h, cooling them to 35° C., and obtaining a mixed raw material;

[0043] S2, adding cellulase to the mixed raw materials and performing enzymatic hydrolysis for 15 min;

[0044] S3, heating the raw material treated in S2 to 80°C, reflux and extract for 4 hours, cooling to room temperature, filtering and collecting the filtrate;

[0045] S4, concentrating the filtrate obtained in S3 to 1 / 3 of the original volume, then adding petroleum ether in a volume ratio of 1:1, ultrasonicating for 5 minutes, and then standing for 20 minutes to separate the organic phase and the aqueous phase, repeatedly extracting the obtained aqueous phase with 1:1 petroleum ether twice, collecting the aqueous phase, evaporating and concentrating, and vacuum freeze-drying to obtain the plant extract.

[0046] The preparation method of the above-mentioned moisturizing composition comprises the following steps:

[0047] S1. Mix algooligosaccharide, pyrrolidone carboxylic acid and polyol, heat to 55°C, homogenize, keep warm to defoam, and cool down to 45°C;

[0048] S2. Sequentially add ceramide, filtrate of Saccharomyces cerevisiae ferment filtrate and plant extract, stir and mix evenly to obtain the moisturizing composition.

[0049] Example 2

[0050] A moisturizing composition, by weight, comprises the following components: 20 parts of plant extract, 15 parts of ceramide, 10 parts of algooligosaccharide, 0.5 part of pyrrolidone carboxylic acid, 3 parts of filtrate of Saccharomyces cerevisiae ferment filtrate and 40 parts of polyol; the plant extract is the extract of Sedum rubrotinctum, Centaurea cyanus, Centella asiatica, Rosa laevigata and Aloe vera, the ceramide is a composition composed of ceramide 1, ceramide AP and ceramide 6II, and the mass ratio of ceramide 1, ceramide AP and ceramide 6II is 1:4:0.1, and the polyol is glycerol and butanediol.

[0051] The preparation method of the plant extract comprises the following steps:

[0052] S1. Crush Sedum rubrotinctum, Centaurea cyanus, Centella asiatica, Rosa laevigata and Aloe vera into powder with a pulverizer, mix them in a mass ratio of 1:1:1:1:1, add 1,2 - hexanediol and water, soak in hot water at 70°C for 6 h, and cool down to 35°C to obtain a mixed raw material;

[0053] S2. Add cellulase to the mixed raw material and carry out enzymatic hydrolysis for 15 min;

[0054] S3. Heat the raw material treated in S2 to 90°C, carry out reflux extraction for 3 h, cool to room temperature, filter, and collect the filtrate;

[0055] S4. Concentrate the filtrate obtained in S3 to 1 / 3 of the original volume, then add petroleum ether with a volume ratio of 1:1, ultrasonicate for 10 min, then let it stand for 10 - 30 min, separate the organic phase and the aqueous phase, repeat the extraction of the obtained aqueous phase with petroleum ether of 1:1 twice, collect the aqueous phase, evaporate and concentrate, and vacuum freeze-dry to obtain the plant extract.

[0056] The preparation method of the above moisturizing composition comprises the following steps:

[0057] S1. Mix algooligosaccharide, pyrrolidone carboxylic acid and polyol, heat to 50°C, homogenize, keep warm to defoam, and cool down to 40°C;

[0058] S2. Sequentially add ceramide, filtrate of Saccharomyces cerevisiae ferment filtrate and plant extract, stir and mix evenly to obtain the moisturizing composition.

[0059] Example 3

[0060] A hydrating and moisturizing composition, comprising the following components by weight: 30 parts of plant extract, 10 parts of ceramide, 5 parts of algal oligosaccharides, 1 part of pyrrolidone carboxylic acid, 7 parts of galactosaccharide yeast fermentation product filtrate and 50 parts of polyols; the plant extract is an extract of red cabbage, cornflower flower, Centella asiatica, Rosa laevigata and aloe vera; the ceramide is a composition consisting of ceramide 1, ceramide AP and ceramide 6II; the mass ratio of ceramide 1, ceramide AP and ceramide 6II is 2:1:1; the polyols are glycerol and butylene glycol.

[0061] The preparation method of the plant extract comprises the following steps:

[0062] S1, crushing red cabbage, cornflower, Centella asiatica, Rosa laevigata, and aloe into powder using a grinder, mixing them in a mass ratio of 1.5:1:1:0.5:1, adding 1,2-hexanediol and water, soaking them in 70° C. hot water for 6 hours, cooling them to 35° C., and obtaining a mixed raw material;

[0063] S2, adding cellulase to the mixed raw materials and performing enzymatic hydrolysis for 15 min;

[0064] S3, heating the raw material treated in S2 to 85°C, reflux and extract for 3h, cooling to room temperature, filtering and collecting the filtrate;

[0065] S4, concentrating the filtrate obtained in S3 to 1 / 3 of the original volume, then adding petroleum ether in a volume ratio of 1:1, ultrasonicating for 7 minutes, and then standing for 15 minutes to separate the organic phase and the aqueous phase, repeatedly extracting the obtained aqueous phase with 1:1 petroleum ether twice, collecting the aqueous phase, evaporating and concentrating, and drying to obtain the plant extract.

[0066] The preparation method of the above-mentioned moisturizing composition comprises the following steps:

[0067] S1, mixing algal oligosaccharides, pyrrolidone carboxylic acid and polyol, heating to 60°C, homogenizing, keeping warm to defoam, and cooling to 40°C;

[0068] S2, sequentially adding ceramide, filtrate of galactosyl yeast fermentation product and plant extract, stirring and mixing evenly, to obtain the hydrating and moisturizing composition.

[0069] Example 4

[0070] A moisturizing composition, by weight, comprises the following components: 30 parts of plant extract, 15 parts of ceramide, 5 parts of algooligosaccharides, 1 part of pyrrolidone carboxylic acid, 5 parts of filtrate of Saccharomyces cerevisiae ferment filtrate, 1.5 parts of phytosphingosine, and 60 parts of polyol; the plant extract is an extract of Sedum rubrotinctum, Centaurea cyanus, Centella asiatica, Rosa laevigata, and Aloe vera, the ceramide is a composition composed of ceramide 1, ceramide AP, and ceramide 6II, and the mass ratio of ceramide 1, ceramide AP, and ceramide 6II is 1:3:1; the polyol is glycerol and butanediol.

[0071] The preparation method of the plant extract comprises the following steps:

[0072] S1, pulverize Sedum rubrotinctum, Centaurea cyanus, Centella asiatica, Rosa laevigata, and Aloe vera into powder with a pulverizer, mix them according to the mass ratio of 1:1:1:1:2, add 1,2 - hexanediol and water, soak in hot water at 70 °C for 5 h, and cool down to 35 °C to obtain a mixed raw material;

[0073] S2, add cellulase to the mixed raw material and perform enzymatic hydrolysis for 20 min;

[0074] S3, heat the raw material treated in S2 to 90 °C, reflux and extract for 5 h, cool down to room temperature, filter, and collect the filtrate;

[0075] S4, concentrate the filtrate obtained in S3 to 1 / 3 of the original volume, then add petroleum ether with a volume ratio of 1:1, ultrasonicate for 10 min, then let it stand for 20 min, separate the organic phase and the aqueous phase, repeat the extraction of the obtained aqueous phase with petroleum ether of 1:1 twice, collect the aqueous phase, evaporate, concentrate, and dry to obtain the plant extract.

[0076] The preparation method of the above moisturizing composition comprises the following steps:

[0077] S1, mix algooligosaccharide, pyrrolidone carboxylic acid, and polyol, heat to 60 °C, homogenize, keep warm to defoam, and cool down to 45 °C;

[0078] S2, sequentially add ceramide, filtrate of Saccharomyces cerevisiae ferment filtrate, and plant extract, stir and mix evenly to obtain the moisturizing composition.

[0079] Example 5

[0080] A moisturizing composition comprises the following components by weight: 25 parts of plant extract, 20 parts of ceramide, 8 parts of algal oligosaccharides, 1.5 parts of pyrrolidone carboxylic acid, 3 parts of galactosaccharide yeast fermentation product filtrate, 2 parts of tremella polysaccharide and 60 parts of polyol; the plant extract is an extract of red vegetable, cornflower flower, Centella asiatica, Rosa laevigata and aloe vera; the ceramide is a composition consisting of ceramide 1, ceramide AP and ceramide 6II; the mass ratio of ceramide 1, ceramide AP and ceramide 6II is 1:3:0.5; the polyol is glycerol and butylene glycol.

[0081] The preparation method of the plant extract comprises the following steps:

[0082] S1, crushing red cabbage, cornflower, Centella asiatica, Rosa laevigata, and aloe into powder using a grinder, mixing them in a mass ratio of 1:1:1:1:1, adding 1,2-hexanediol and water, soaking them in 80° C. hot water for 5 h, and cooling them to 35° C. to obtain a mixed raw material;

[0083] S2, adding cellulase to the mixed raw materials and performing enzymatic hydrolysis for 15 min;

[0084] S3, heating the raw material treated in S2 to 80°C, reflux and extract for 4 hours, cooling to room temperature, filtering and collecting the filtrate;

[0085] S4, concentrating the filtrate obtained in S3 to 1 / 3 of the original volume, then adding petroleum ether in a volume ratio of 1:1, ultrasonicating for 10 minutes, and then standing for 15 minutes to separate the organic phase and the aqueous phase, repeatedly extracting the obtained aqueous phase with 1:1 petroleum ether twice, collecting the aqueous phase, evaporating and concentrating, and drying to obtain the plant extract.

[0086] The preparation method of the above-mentioned moisturizing composition comprises the following steps:

[0087] S1, mixing algal oligosaccharides, pyrrolidone carboxylic acid and polyols, heating to 60°C, homogenizing, keeping warm to defoam, and cooling to 45°C;

[0088] S2, sequentially adding ceramide, filtrate of galactosyl yeast fermentation product and plant extract, stirring and mixing evenly, to obtain the hydrating and moisturizing composition.

[0089] Example 6

[0090] A moisturizing composition comprises the following components by weight: 35 parts of plant extract, 20 parts of ceramide, 10 parts of algal oligosaccharides, 1 part of pyrrolidone carboxylic acid, 5 parts of galactosaccharide yeast fermentation product filtrate, 2 parts of tremella polysaccharide, 1 part of phytosphingosine and 60 parts of polyols; the plant extract is an extract of red vegetable, cornflower flower, Centella asiatica, Rosa laevigata and aloe vera; the ceramide is a composition consisting of ceramide 1, ceramide AP and ceramide 6II; the mass ratio of ceramide 1, ceramide AP and ceramide 6II is 1:4:0.5; the polyol is glycerol and butylene glycol.

[0091] The preparation method of the plant extract comprises the following steps:

[0092] S1, crushing red cabbage, cornflower, Centella asiatica, Rosa laevigata, and aloe into powder using a grinder, mixing them in a mass ratio of 1:1:1:1:1, adding 1,2-hexanediol and water, soaking them in 80° C. hot water for 7 h, cooling them to 35° C., and obtaining a mixed raw material;

[0093] S2, adding cellulase to the mixed raw materials and performing enzymatic hydrolysis for 20 min;

[0094] S3, heating the raw material treated in S2 to 95°C, reflux and extract for 6 hours, cooling to room temperature, filtering and collecting the filtrate;

[0095] S4, concentrating the filtrate obtained in S3 to 1 / 3 of the original volume, then adding petroleum ether in a volume ratio of 1:1, ultrasonicating for 10 minutes, and then standing for 20 minutes to separate the organic phase and the aqueous phase, repeatedly extracting the obtained aqueous phase with 1:1 petroleum ether twice, collecting the aqueous phase, evaporating and concentrating, and drying to obtain the plant extract.

[0096] The preparation method of the above-mentioned moisturizing composition comprises the following steps:

[0097] S1, mixing algal oligosaccharides, pyrrolidone carboxylic acid and polyol, heating to 55°C, homogenizing, keeping warm to defoam, and cooling to 45°C;

[0098] S2, sequentially adding ceramide, filtrate of galactosyl yeast fermentation product and plant extract, stirring and mixing evenly, to obtain the hydrating and moisturizing composition.

[0099] Comparative Example 1

[0100] The difference from Example 1 is that the plant extract is not included and is replaced by an equal amount of water.

[0101] Comparative Example 2

[0102] The difference from Example 1 is that ceramide is not included and is replaced by an equal amount of water.

[0103] Comparative Example 3

[0104] The difference from Example 1 is that the filtrate of the fermentation product of galactosyl yeast is not included, and is replaced by an equal amount of water.

[0105] Comparative Example 4

[0106] The difference from Example 1 is that algal oligosaccharides are not included and are replaced by an equal amount of water.

[0107] Comparative Example 5

[0108] The difference from Example 1 is that pyrrolidone carboxylic acid is not contained and is replaced by an equal amount of water.

[0109] Comparative Example 6

[0110] The difference from Example 1 is that no polyol is included and an equal amount of water is used instead.

[0111] Comparative Example 7

[0112] The difference from Example 1 is that the plant extract and ceramide are not included, and an equal amount of water is used instead.

[0113] Comparative Example 8

[0114] The difference from Example 1 is that the plant extract, ceramide and algae oligosaccharides are not included, and are replaced by an equal amount of water.

[0115] Comparative Example 9

[0116] The difference from Example 1 is that algal oligosaccharides, pyrrolidone carboxylic acid and polyol are not included, and an equal amount of water is used instead.

[0117] Comparative Example 10

[0118] The difference from Example 1 is that the plant extract, the filtrate of the fermentation product of galactosyl yeast and the polyol are not contained, and are replaced by an equal amount of water.

[0119] Comparative Example 11

[0120] The moisturizing composition provided in this comparative example includes the following components by weight: 5 parts of plant extract, 30 parts of ceramide, 2 parts of algal oligosaccharides, 0.1 parts of pyrrolidone carboxylic acid, 2 parts of galactosyl yeast fermentation product filtrate and 10 parts of polyols; the plant extract is an extract of red cabbage, cornflower flower, Centella asiatica, Rosa laevigata and aloe vera.

[0121] Sample preparation example

[0122] A cream containing a moisturizing composition is made of the following components: 3.0 g of butylene glycol, 0.05 g of disodium EDTA, 7 g of glycerol, 1 g of hyaluronic acid, 0.1 g of xanthan gum, 3.9 g of isoisotridecyl isononanoate, 1.3 g of jojoba seed oil, 1.3 g of squalane, 1.3 g of hydrogenated microcrystalline wax, 0.05 g of cetyl alcohol, 0.07 g of methyl glucoside sesquistearate, 3 g of polydimethylsiloxane, 0.8 g of polyglyceryl-3 methylglucose distearate, 10 g of the moisturizing composition, 0.5 g of p-hydroxyacetophenone, 0.03 g of essence, and 68.52 g of deionized water.

[0123] To verify the moisturizing effect of the composition of the present invention, the following tests were carried out:

[0124] (I) Safety test

[0125] The compositions prepared in all the above examples and comparative examples were subjected to a human safety patch test. The test method referred to the human skin patch test in the "2015 Cosmetics Safety and Technology Specifications". The method of the skin closed patch test was as follows: 45 persons aged 30 - 55 years old were selected. Qualified patch test devices with an area not exceeding 50 mm 2 and a depth of about 1 mm were used. 0.020 mL of the compositions prepared in the above examples and comparative examples was taken and placed in the small chamber of the patch tester. The control hole was a blank control (without placing any substance). The patch tester with the added repair and firming composition was applied to the flexor side of the forearm of the subject with a low-allergy tape, and gently pressed with the palm to make it evenly adhere to the skin for 24 h. The skin reactions were observed according to the criteria in Table 1 at 30 min (after the indentation disappeared), 24 h, and 48 h respectively after removing the patch tester, and the observation results were recorded.

[0126] Table 1 Observation criteria

[0127]

[0128] Patch test results: No skin positive reactions occurred within 30 min, 24 h, and 4 h after removing the patch tester. Thus, it can be seen that the composition of the present invention is mild and safe for humans and can be safely added to cosmetics for use.

[0129] (II) Skin moisturizing test

[0130] 1. Short-term moisturizing test

[0131] Test method: 170 volunteers aged 30 - 50 years old, female, with daily skin care habits, and meeting the inclusion and exclusion criteria of the "Cosmetics Safety and Technology Specifications" were recruited. The volunteers were randomly and evenly divided into 17 groups, with 10 persons in each group. Cosmetics prepared by using the long-acting moisturizing compositions prepared in Examples 1 - 6 and Comparative Examples 1 - 11 were used respectively.

[0132] The water content of the stratum corneum was measured using a Corneometer CM825 (Courage Khazaka, Germany). On the flexor sides of the left and right forearms of the subjects, test areas of 4 cm × 4 cm were marked, and each sample was applied. The application amount was 1.0 mg / cm 2 , and gently massaged until the sample was absorbed. The skin water content before application and 2 hours and 6 hours after application was measured using a skin moisture meter. The skin water content increase rate was calculated according to the following formula, and the average skin water content increase rate of the volunteers using each sample was taken as the average skin water content increase rate of the sample. Before the test, the subjects' faces were uniformly cleaned and they rested in a thermostatic and humidified chamber for 30 min. Skin water content increase rate (%) = (measured value after application - baseline value before application) / baseline value before application × 100%. The test results are shown in Table 2.

[0133] Table 2 Effects of the compositions on short-term skin moisturization

[0134]

[0135]

[0136] Note: T0 is 0 hour before the test, T2 is 2 hours after application, and T6 is 6 hours after application.

[0137] It can be seen from the test results in Table 2 that the short-term skin moisturization effects of Examples 1-6 containing the moisturizing composition of the present invention are better than those of Comparative Examples 1-11. In Examples 4 and 5, the components phytosphingosine and tremella polysaccharide were newly added, which are better than Examples 1-3, indicating that the components phytosphingosine and tremella polysaccharide can further enhance the moisturizing effect of the composition. In Example 6, the components phytosphingosine and tremella polysaccharide were newly added at the same time, and the moisturizing effect is better than that of Examples 4 and 5 because phytosphingosine and tremella polysaccharide can coordinately enhance the moisturizing effect together with other components.

[0138] Compared with Example 1, Comparative Examples 1-6 each lacked one component, and the moisturizing effect decreased to varying degrees, indicating that the plant extracts, ceramides, algooligosaccharides, pyrrolidone carboxylic acid, filtrate of Saccharomyces cerevisiae fermentation products, and polyols in the composition provided by the present invention synergistically exerted a moisturizing effect. The absence of any component would reduce the moisturizing efficacy. Comparative Example 7 lacked both plant extracts and ceramides, and the effect was further weakened. Comparative Examples 8-10 lacked three components simultaneously, and the moisturizing effect decreased significantly. In Comparative Example 11, the content ratio of each component was significantly adjusted, resulting in a significantly worse final moisturizing effect. Comparative Example 11 had a high proportion of ceramides, but due to the lack of other component cooperation, a better moisturizing effect could not be achieved. Thus, the components provided by the present invention need to maintain a reasonable and appropriate ratio, coordinate with each other, and jointly synergistically enhance the moisturizing effect.

[0139] 2. Long-term Moisturizing Test

[0140] Test method: 100 volunteers aged 30-50 years old, female, with daily skincare habits, who met the inclusion and exclusion criteria of the "Cosmetics Safety and Technology Specifications" were recruited. The volunteers were randomly and evenly divided into 10 groups and used cosmetics prepared from the long-term moisturizing compositions prepared in Example 1, Example 4, Example 6, and Comparative Examples 1-3, Comparative Examples 7-8, and Comparative Examples 10-11, respectively.

[0141] The Corneometer CM825 (Courage Khazaka, Germany) was used to measure the water content of the skin stratum corneum, and the Tewameter TM Hex was used to measure the transepidermal water loss rate of the skin. 1.0 mL of the cosmetic was evenly applied to the cheek area of the subjects, and it was used once in the morning and once in the evening for 30 consecutive days. Before the test, the subjects' faces were uniformly cleaned and they rested in a constant temperature and humidity chamber for 30 min. The relative increase in the water content of the skin stratum corneum was calculated according to the following formula: Relative increase in the water content of the skin stratum corneum (%) = (Measured value after application - Measured value before application) / Measured value before application × 100%. The greater the relative increase in the water content of the skin stratum corneum (%), the better the moisturizing effect. The improvement rate of transepidermal water loss (%) was calculated according to the following formula: Improvement rate of transepidermal water loss (%) = (1 - Measured value after application / Measured value before application) × 100%. The greater the improvement rate of transepidermal water loss (%), the better the moisturizing effect. The skin water loss amount TEWL is an important parameter for evaluating the function of the skin water protection layer and can be used to evaluate the strength of the skin barrier function.

[0142] Table 3 Influence of the Composition on Long-term Moisturizing of the Skin

[0143]

[0144] As can be seen from Table 3, after applying the cream samples containing the moisturizing compositions provided in Examples 1, 4, and 6 for 30 days, the water content of the skin stratum corneum increased by 63.14% - 84.29%. For the samples of Comparative Examples 1 - 3, Comparative Examples 7 - 8, and Comparative Examples 10 - 11, the water content of the skin stratum corneum only increased by 6.95% - 39.38%. It shows that the samples of the present invention have the effect of improving the skin moisture content. Through the test of the skin transepidermal water loss rate, the reduction in the amount of skin water loss in the groups of Examples 1, 4, and 6 of the present invention is greater, significantly higher than that of the comparative examples, indicating that the compositions of Examples 1, 4, and 6 have less water loss during the long-term moisturizing process, indicating that the composition provided by the present invention can form a stable long-acting water-locking barrier on the skin surface.

[0145] Although the embodiments of the present specification have been shown and described, for those of ordinary skill in the art, it can be understood that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principles and spirit of the present specification. The scope of the present specification is defined by the appended claims and their equivalents.

Claims

1. A moisturizing composition, characterized in that: The invention comprises the following components by weight: 20-40 parts of plant extract, 10-25 parts of ceramide, 5-15 parts of algae oligosaccharides, 1-8 parts of galactose yeast-like bacteria fermentation product filtrate, 0.5-2 parts of pyrrolidone carboxylic acid and 35-60 parts of polyols; the plant extract is an extract of red succulent vegetable, cornflower flower, Centella asiatica, Rosa laevigata and aloe vera; the mass ratio of the red succulent vegetable, cornflower flower, Centella asiatica, Rosa laevigata and aloe vera is (1-2):(1-2):(1-2):(2-3), and the active ingredients are extracted after mixing according to this ratio; the polyol is glycerol and butylene glycol; the preparation method of the plant extract comprises the following steps: S1, crushing the red succulent vegetable, cornflower flower, Centella asiatica, Rosa laevigata and aloe vera into powder by a pulverizer, adding 1,2-hexanediol and water to mix, and soaking in hot water at 60-80°C Soak for 4-8h, cool to 30-35℃ to obtain a mixed raw material; S2, add cellulase to the mixed raw material, and perform enzymolysis for 10-20min; S3, heat the raw material treated by S2 to 80-95℃, reflux and extract for 3-6h, cool to room temperature, filter, and collect the filtrate; S4, concentrate the filtrate obtained in S3 to 1 / 4-1 / 2 of the original volume, then add petroleum ether with a volume ratio of 1:1, ultrasonicate for 5-10min, then stand for 10-30min, separate the organic phase and the aqueous phase, extract the obtained aqueous phase with 1:1 petroleum ether repeatedly for 1-2 times, collect the aqueous phase, evaporate and concentrate, and dry to obtain the plant extract; the amount of cellulase added in S2 is 0.1-1.5‰ of the mixed raw material; the drying method in S4 is freeze-drying, the freeze-drying temperature is 60-70℃, the time is 3-8h, and freeze-dried powder is prepared.

2. The moisturizing composition according to claim 1, characterized in that: The ceramide is a composition consisting of ceramide 1, ceramide AP and ceramide 6II, and the mass ratio of ceramide 1, ceramide AP and ceramide 6II is (1-3): (2-5): (0.05-1).

3. The moisturizing composition according to claim 1, characterized in that: The invention also contains 0.5-5 parts of Tremella polysaccharide and / or 0.1-4 parts of phytosphingosine.

4. The method for preparing the moisturizing composition according to claim 1, characterized in that: The following steps are involved: S1, mixing algal oligosaccharides and polyols, heating to 50-70°C, homogenizing, keeping warm to defoam, and cooling down by 40-50°C; S2, sequentially adding ceramide, filtrate of galactosyl yeast fermentation product, pyrrolidone carboxylic acid and plant extract, stirring and mixing evenly, to obtain the hydrating and moisturizing composition.

5. Use of the hydrating and moisturizing composition according to any one of claims 1 to 3 or the hydrating and moisturizing composition obtained by the preparation method of claim 4 in the preparation of cosmetics, characterized in that: The cosmetics include lotion, emulsion, cream, facial mask or essence.

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