Embryonic stem cell bionic culture system and culture method

Abstract

The present invention discloses an embryonic stem cell bionic culture system and a culture method in the field of cell culture technology, comprising a culture box, wherein a plurality of equally spaced pull-out plates are slidably connected inside the culture box, a sealing plate is arranged on the top of the pull-out plate, and the top of the sealing plate is rotatably connected to the culture box, and a transmission support component is arranged on the pull-out plate, and the transmission support component is used to automatically open the sealing plate when the pull-out plate is stretched out from the inside of the culture box. The present invention adopts the transmission support component, so that the sealing plate can be opened simultaneously with the pull-out plate being stretched, and cooperates with the stretching fixing component to provide supporting force for the pull-out plate, so that the pull-out plate can have sufficient supporting force to keep it stable even after being fully stretched from the inside of the culture box. The fully stretchable pull-out plate designed in this way can not only facilitate the taking and placing of culture dishes, but also effectively ensure the stability of the culture dishes.

Description

Technical Field

[0001] The invention relates to the technical field of cell culture, in particular to an embryonic stem cell bionic culture system and a culture method. Background Art

[0002] Embryonic stem cells are a type of cells isolated from early embryos (before the gastrula stage) or primitive gonads. They have the characteristics of unlimited proliferation, self-renewal and multidirectional differentiation in vitro. Whether in vitro or in vivo, ES cells can be induced to differentiate into almost all cell types in the body. Cell culture refers to a method of simulating the in vivo environment (sterility, suitable temperature, pH and certain nutritional conditions, etc.) in vitro to enable them to survive, grow, reproduce and maintain their main structures and functions.

[0003] When culturing embryonic stem cells, the embryonic stem cells need to be separated and placed in a culture dish filled with nutrients, and then the culture dish is placed in an incubator. The incubator is controlled to provide an environment that the embryonic stem cells are adapted to so that they can grow. Usually, multiple groups of embryonic stem cells are cultured in batches in the incubator for comparison. If a relatively flat culture dish is used to culture multiple groups of embryonic stem cells, and the incubator is relatively narrow and long, it will be inconvenient to take the culture dish close to the inside of the incubator. If mistakes are made during the process of taking and placing the culture dish, it is likely to cause cell death.

[0004] Based on this, the present invention designs an embryonic stem cell bionic culture system and culture method to solve the above-mentioned problem of being inconvenient to take and place culture dishes in a narrow and long culture box. Summary of the invention

[0005] The object of the present invention is to provide an embryonic stem cell bionic culture system and culture method to solve the problems raised in the above background technology.

[0006] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: an embryonic stem cell bionic culture system, comprising an incubator, wherein a plurality of equally spaced pull-out plates are slidably connected inside the incubator, a sealing plate is arranged on the top of the pull-out plate, the top of the sealing plate is rotatably connected to the incubator, a transmission support assembly is arranged on the pull-out plate, and the transmission support assembly is used to automatically open the sealing plate when the pull-out plate is stretched out from the inside of the incubator, and the sealing plate can be separated from the transmission of the pull-out plate after being opened to a certain angle so that the pull-out plate can continue to slide.

[0007] As a further solution of the present invention, the transmission support assembly includes a limit rod, which is fixed on both sides of the top of the pull-out plate, and a block is fixedly connected to the top of the pull-out plate on the front side of the limit rod, and a roller is arranged between the limit rod and the block, and a push rod is rotatably connected to the side wall of the roller, and the front end of the push rod is rotatably connected to the outer wall of the rear end of the sealing plate, and a fixing frame is fixedly connected to the inner wall of the incubator at the top of the roller, and a stretching fixing assembly is arranged on the pull-out plate, and the stretching fixing assembly is used to improve the stability of the pull-out plate after the pull-out plate is fully stretched from the inside of the incubator.

[0008] As a further solution of the present invention, the stretching and fixing assembly includes a first slide rail, which is opened on the outer wall at the rear end of the sealing plate, a slider is slidably connected inside the first slide rail, a telescopic rod is rotatably connected to the slider, a fixed seat is fixedly connected to the top of the pull-out plate, the output end of the telescopic rod is rotatably connected to the fixed seat, a first spring for resetting the telescopic rod is sleeved on the telescopic rod, and a limited support assembly is provided at the front end of the incubator, the limited support assembly is used to limit the pull-out plate from sliding toward the front side of the incubator, and further provide support force for the pull-out plate when the pull-out plate slides toward the front side of the incubator.

[0009] As a further solution of the present invention, the limit support assembly includes a connecting plate, which is fixed to the outer wall of the front end of the incubator, a fixing frame is provided at the bottom of the connecting plate, and a cone plate is slidably connected to the right end of the fixing frame, and a second spring for resetting the cone plate is fixedly connected to the inside of the fixing frame, and a second slide groove for docking with the cone plate is provided on the side wall at the left end of the pull-out plate, the top of the fixing frame is fixedly connected to a connecting seat, a swing rod is provided at the top of the fixing frame, the bottom of the swing rod is rotatably connected to the connecting seat, a slide rail is provided at the right end of the connecting plate, the top of the swing rod is slidably connected to the slide rail, the front end of the connecting plate is rotatably connected to the limit plate, the swing rod is provided with an opening for docking with the limit plate, and a third spring for resetting the limit plate is fixedly connected to the inside of the connecting plate.

[0010] As a further solution of the present invention, a handle is fixedly connected to the front end of the pull-out plate, and an anti-slip pad with a large friction coefficient is sleeved on the outside of the handle.

[0011] As a further solution of the present invention, both ends of the top of the sealing plate are respectively fixedly connected to a rotating shaft, and a groove rotatably connected to the rotating shaft is provided inside the incubator.

[0012] As a further solution of the present invention, a plurality of pads distributed at equal distances are fixedly connected to the top of the pull-out plate, and a plurality of air holes distributed at equal distances are opened inside the pull-out plate.

[0013] As a further solution of the present invention, a plurality of heat dissipation holes which are evenly distributed are provided at the bottom of the front end of the incubator, and acrylic plates are fixedly connected to both sides of the sealing plate.

[0014] A bionic culture method for embryonic stem cells, the method comprising the following steps:

[0015] Step 1: Stretch the pull-out plate outwards and flip the sealing plate open synchronously through the transmission support assembly;

[0016] Step 2: Place the prepared cell culture dishes on top of the pull-out plate one by one;

[0017] Step 3: Push the pull-out plate back into the incubator, and the transmission support assembly will close the sealing plate synchronously;

[0018] Step 4: Start the incubator and set the corresponding ambient temperature to culture the cells.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] The present invention adopts a transmission support component so that the cover plate can be opened while the pull-out plate is stretched, and cooperates with the stretching fixing component to provide support force for the pull-out plate, so that the pull-out plate can have sufficient support force to keep it stable even after it is fully stretched from the inside of the incubator. The pull-out plate designed in this way that can be fully stretched can facilitate the removal and placement of the culture dish and effectively ensure the stability of the culture dish. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0022] Figure 2 This is a schematic diagram of the internal structure of the incubator of the present invention;

[0023] Figure 3 It is a schematic diagram of the transmission support assembly structure;

[0024] Figure 4 for Figure 3 The enlarged structural diagram at A in the middle;

[0025] Figure 5 It is a schematic diagram of the structure of the limit support component;

[0026] Figure 6 for Figure 5 The enlarged structural diagram at B in the middle;

[0027] Figure 7 The figure is a flow chart of the method of the present invention.

[0028] In the accompanying drawings, the components represented by the reference numerals are listed as follows:

[0029] 1. Incubator; 2. Heat dissipation holes; 3. Pull-out plate; 4. Handle; 5. Closing plate; 6. Acrylic plate; 7. Rotating shaft; 8. Slider; 9. First slide rail; 10. Telescopic rod; 11. First spring; 12. Fixed seat; 13. Pad; 14. Air vent; 15. Limit rod; 16. Fixed frame; 17. Push rod; 18. Second slide groove; 19. Block; 20. Rocker; 21. Connecting plate; 22. Fixed frame; 23. Second spring; 24. Conical plate; 25. Connecting seat; 26. Limit plate; 27. Third spring; 28. Roller. DETAILED DESCRIPTION

[0030] See also Figure 1-Figure 7 The present invention provides a technical solution: an embryonic stem cell bionic culture system, comprising a culture box 1, wherein a plurality of equally spaced pull-out plates 3 are slidably connected inside the culture box 1, a sealing plate 5 is arranged at the top of the pull-out plate 3, and the top of the sealing plate 5 is rotatably connected to the culture box 1, and a transmission support assembly is arranged on the pull-out plate 3, wherein the transmission support assembly is used to automatically open the sealing plate 5 when the pull-out plate 3 is pulled out from the culture box 1, and the sealing plate 5 can be separated from the transmission of the pull-out plate 3 after opening a certain angle so that the pull-out plate 3 can continue to slide;

[0031] When the above scheme is put into actual use, during cell culture, the separated embryonic stem cells are placed in the culture dish, the pull-out plate 3 is stretched outward, the sealing plate 5 is flipped open synchronously by the transmission support assembly, the prepared cell culture dishes are placed on the top of the pull-out plate 3 in turn, the pull-out plate 3 is pushed back into the incubator 1, the transmission support assembly will synchronously close the sealing plate 5, the incubator 1 is started and the corresponding ambient temperature is set to culture the cells, and when one or more culture dishes need to be taken out, the above action is repeated to pull open the pull-out plate 3 to take out the corresponding culture dishes. The advantage of this is that when the pull-out plate 3 is taken out, the sealing plate 5 is synchronously opened by the transmission support assembly, so that the sealing plate 5 will not form an obstruction, and there will be more space for taking in and placing the culture dishes.

[0032] As a further solution of the present invention, the transmission support assembly includes a limit rod 15, the limit rod 15 is fixed on both sides of the top of the pull-out plate 3, the top of the pull-out plate 3 on the front side of the limit rod 15 is fixedly connected with a stopper 19, a roller 28 is arranged between the limit rod 15 and the stopper 19, a push rod 17 is rotatably connected to the side wall of the roller 28, the front end of the push rod 17 is rotatably connected to the outer wall of the rear end of the sealing plate 5, a fixing frame 16 is fixedly connected to the inner wall of the incubator 1 at the top of the roller 28, a stretching and fixing assembly is arranged on the pull-out plate 3, the stretching and fixing assembly is used to improve the stability of the pull-out plate 3 after the pull-out plate 3 is fully stretched from the inside of the incubator 1, the top ends of the sealing plate 5 are respectively fixedly connected with a rotating shaft 7, and a groove rotatably connected to the rotating shaft 7 is opened inside the incubator 1;

[0033] When the above scheme is put into actual use, when the drawer plate 3 is stretched outward, the fixing frame 16 will not slide and displace with the drawer plate 3, but the roller 28 will slide with the drawer plate 3 under the limiting action of the limiting rod 15, and the sliding of the roller 28 will push the closing plate 5 to rotate around the rotating shaft 7 through the push rod 17, so that the sliding of the drawer plate 3 and the closing plate 5 are driven to rotate and open synchronously, and as the drawer plate 3 slides, when the roller 28 slides to the bottom of the notch at the front end of the fixing frame 16, the front end of the fixing frame 16 will contact with the roller 28 to limit the roller 28 from continuing to slide, and the drawer plate 3 continues to slide, and the front end of the fixing frame 16 will push the roller 28 to rotate When the column 28 is pushed into the notch of the fixing frame 16, and the bottom of the roller 28 enters the notch, the bottom of the roller 28 contacts the top of the second slide groove 18. At this time, when the drawer plate 3 slides again, the roller 28 will only rotate above the limit rod 15 and will not continue to slide with the limit rod 15. At this time, the cover plate 5 will not continue to flip over. The purpose of this is to separate the cover plate 5 from the drawer plate 3 after it is opened, and the drawer plate 3 can still continue to slide. This ensures that the drawer plate 3 can slide a long enough distance, so that the culture dishes inside the drawer plate 3 are all exposed to the outside of the incubator 1, which is convenient for taking out.

[0034] When the culture dish is taken out, the pull-out plate 3 is pushed back into the incubator 1. When the front end of the limit rod 15 slides to the bottom of the groove on the fixing frame 16, the roller 28 will re-enter between the stop block 19 and the limit rod 15 under the action of gravity. Due to the restriction of the stop block 19, when the pull-out plate 3 slides back, it drives the sealing plate 5 to rotate and seal from the front end of the incubator 1 through the push rod 17, thus completing a cycle. The overall device drives the sealing plate 5 to flip and open while the pull-out plate 3 is stretched outward, and after sliding a certain distance, the pull-out plate 3 disengages from the transmission of the sealing plate 5 so that the sealing plate 5 remains open, and the pull-out plate 3 continues to stretch outward, so that all the culture dishes on the pull-out plate 3 can be taken out from the incubator 1.

[0035] As a further solution of the present invention, the stretching and fixing assembly includes a first slide rail 9, which is provided on the outer wall at the rear end of the sealing plate 5, and a slider 8 is slidably connected inside the first slide rail 9, and a telescopic rod 10 is rotatably connected to the slider 8, and a fixing seat 12 is fixedly connected to the top of the pull-out plate 3, and the output end of the telescopic rod 10 is rotatably connected to the fixing seat 12, and a first spring 11 for resetting the telescopic rod 10 is sleeved on the telescopic rod 10, and a limited support assembly is provided at the front end of the incubator 1, and the limited support assembly is used to limit the pull-out plate 3 from sliding toward the front side of the incubator 1, and further provide support force for the pull-out plate 3 when the pull-out plate 3 slides toward the front side of the incubator 1;

[0036] When the above scheme is put into actual use, when the drawer plate 3 is stretched outward, the front end of the drawer plate 3 contacts the cone plate 24, pushing the cone plate 24 to compress the second spring 23 and retract it into the fixed frame 22. When the drawer plate 3 slides to the second slide groove 18 and contacts the cone plate 24, the cone plate 24 is pushed into the second slide groove 18 under the elastic force of the second spring 23. If the drawer plate 3 needs to be fully stretched, the limit plate 26 is rotated to a position disengaged from the swing rod 20. When the cone plate 24 slides to one end of the second slide groove 18, continuing to stretch the drawer plate 3 will cause The swing rod 20 slides downward along the connecting plate 21. When the pull-out plate 3 is fully stretched, the swing rod 20 also slides to the bottom along the connecting plate 21. At this time, the pull-out plate 3 can be further supported by the swing rod 20 through the docking of the cone plate 24 and the second slide groove 18. When the pull-out plate 3 is located inside the incubator 1 and is not stretched, the cone plate 24 can limit the sliding of the pull-out plate 3 from the front end. In this way, it can be avoided that the pull-out plate 3 is not fully reset during cell culture, resulting in the failure to isolate the internal and external environments of the incubator 1 and affecting the cell culture.

[0037] As a further solution of the present invention, the limit support assembly includes a connecting plate 21, the connecting plate 21 is fixed on the front outer wall of the incubator 1, a fixing frame 22 is provided at the bottom end of the connecting plate 21, a cone plate 24 is slidably connected to the right end of the fixing frame 22, a second spring 23 for resetting the cone plate 24 is fixedly connected inside the fixing frame 22, a second slide groove 18 for docking with the cone plate 24 is provided on the left side wall of the pull-out plate 3, a connecting seat 25 is fixedly connected to the top of the fixing frame 22, a swing rod 20 is provided at the top of the fixing frame 22, the bottom end of the swing rod 20 is rotatably connected to the connecting seat 25, a slide rail is provided at the right end of the connecting plate 21, the top end of the swing rod 20 is slidably connected to the slide rail, the front end of the connecting plate 21 is rotatably connected to the limit plate 26, the swing rod 20 is provided with an opening for docking with the limit plate 26, and a third spring 27 for resetting the limit plate 26 is fixedly connected inside the connecting plate 21;

[0038] When the above scheme is put into practical use, Figure 3When the drawer 3 is fully extended, the slider 8 is located at the front of the first slide rail 9, and the telescopic rod 10 is also extended to the maximum value. The elastic force of the first spring 11 pulls the cover 5 to make it tend to rotate downward, and the cover 5 transmits these forces to the limit rod 15 through the push rod 17, so that the cover 5 applies pressure to the limit rod 15 through the push rod 17. The purpose of this is that after the drawer 3 is fully extended from the inside of the incubator 1, only the connection between the rear end of the drawer 3 and the incubator 1 is stressed. At this time, the front end of the drawer 3 is suspended as a whole, making the drawer 3 very unstable. By stretching the cover 5 from the front of the drawer 3 by the telescopic rod 10, and then applying force to the limit rod 15 through the cover 5, the stability of the drawer 3 can be further improved.

[0039] As a further solution of the present invention, a handle 4 is fixedly connected to the front end of the pull-out plate 3, and an anti-slip pad with a large friction coefficient is sleeved on the outside of the handle 4;

[0040] When the above solution is put into actual use, the handle 4 can provide a force point, making it easier to pull the pull-out plate 3 to slide inside the incubator 1, while the anti-slip pad further increases the friction force to play an anti-slip role.

[0041] As a further solution of the present invention, a plurality of equally spaced pads 13 are fixedly connected to the top of the pull-out plate 3, and a plurality of equally spaced air holes 14 are provided inside the pull-out plate 3;

[0042] When the above scheme is put into actual use, the position of each culture dish on the pull-out plate 3 is determined by the pad 13, so that the culture dishes can be placed in order, and the air inside the incubator 1 can circulate through the air holes 14 to adjust and control the environment around each pull-out plate 3.

[0043] As a further solution of the present invention, the bottom of the front end of the incubator 1 is provided with a plurality of heat dissipation holes 2 which are evenly distributed, and both sides of the sealing plate 5 are fixedly connected with acrylic plates 6 respectively;

[0044] When the above solution is put into actual use, the heat dissipation holes 2 at the bottom can facilitate heat dissipation of the incubator 1 , and the acrylic plate 6 can be used to observe the culture conditions of the culture dishes from the outside of the incubator 1 .

[0045] A bionic culture method for embryonic stem cells, the method comprising the following steps:

[0046] Step 1: Stretch the pull-out plate 3 outward, and synchronously flip the sealing plate 5 open through the transmission support assembly;

[0047] Step 2: Place the prepared cell culture dishes on the top of the pull-out plate 3 in sequence;

[0048] Step 3: Push the pull-out plate 3 back into the incubator 1, and the transmission support assembly will synchronously close the sealing plate 5;

[0049] Step 4: Start the incubator 1 and set the corresponding ambient temperature to culture the cells.

[0050] Working principle: When conducting cell culture, the separated embryonic stem cells are placed in a culture dish.

[0051] When the draw plate 3 is stretched outward, the fixing frame 16 will not slide with the draw plate 3, but the roller 28 will slide with the draw plate 3 under the limiting action of the limiting rod 15, and the sliding of the roller 28 will push the sealing plate 5 to rotate around the rotating shaft 7 through the push rod 17, so as to realize the sliding of the draw plate 3 and drive the sealing plate 5 to rotate and open synchronously. As the draw plate 3 slides, when the roller 28 slides to the bottom of the notch at the front end of the fixing frame 16, the front end of the fixing frame 16 will contact the roller 28 to limit the roller 28 from continuing to slide, and the draw plate 3 continues to slide. The front end of the fixing frame 16 will push the roller 28 into the notch of the fixing frame 16, and after the roller 28 enters the notch, its bottom will contact the top of the second slide groove 18. At this time, when the draw plate 3 slides again, the roller 28 will only rotate above the limiting rod 15 and will not continue to slide with the limiting rod 15, and the sealing plate 5 will not continue to flip over.

[0052] When the drawer plate 3 is stretched outward, the front end of the drawer plate 3 contacts the cone plate 24, pushing the cone plate 24 to compress the second spring 23 and retract it into the fixed frame 22. When the drawer plate 3 slides to the second slide groove 18 and contacts the cone plate 24, the cone plate 24 is pushed into the second slide groove 18 under the elastic force of the second spring 23. If the drawer plate 3 needs to be fully stretched, the limit plate 26 is rotated to a position disengaged from the swing rod 20. When the cone plate 24 slides to one end of the second slide groove 18, continuing to stretch the drawer plate 3 will cause the swing rod 20 to slide downward along the connecting plate 21. When the drawer plate 3 is fully stretched, the swing rod 20 also slides to the bottom along the connecting plate 21. At this time, the cone plate 24 is connected with the second slide groove 18, and the swing rod 20 can further support the drawer plate 3.

[0053] When the drawer plate 3 is fully extended outwards, the slider 8 is located at the front of the first slide rail 9, and the telescopic rod 10 is also extended to the maximum value, and the sealing plate 5 is pulled by the elastic force of the first spring 11 to have a tendency to rotate downward, and the sealing plate 5 transmits these forces to the limit rod 15 through the push rod 17, so that the sealing plate 5 applies pressure to the limit rod 15 through the push rod 17. The purpose of this is that after the drawer plate 3 is fully extended from the inside of the incubator 1, only the connection between the rear end of the drawer plate 3 and the incubator 1 is stressed. At this time, the front end of the drawer plate 3 is suspended as a whole, making the drawer plate 3 very unstable. By stretching the sealing plate 5 from the front of the drawer plate 3 by the telescopic rod 10, and then applying force to the limit rod 15 through the sealing plate 5, the stability of the drawer plate 3 can be further improved.

Claims

1. A bionic embryonic stem cell culture system, comprising a culture box (1), wherein a plurality of equally spaced pull-out plates (3) are slidably connected to the inside of the culture box (1), a sealing plate (5) is arranged at the top of the pull-out plate (3), and the top of the sealing plate (5) is rotatably connected to the culture box (1), characterized in that: The pull-out plate (3) is provided with a transmission support assembly, and the transmission support assembly is used to automatically open the sealing plate (5) when the pull-out plate (3) is pulled out from the inside of the incubator (1), and the sealing plate (5) can be separated from the transmission of the pull-out plate (3) after opening to a certain angle, so that the pull-out plate (3) can continue to slide; The transmission support assembly comprises a limit rod (15), the limit rod (15) being fixed to both sides of the top end of the pull-out plate (3), the top end of the pull-out plate (3) on the front side of the limit rod (15) being fixedly connected to a stopper (19), a roller (28) being arranged between the limit rod (15) and the stopper (19), a push rod (17) being rotatably connected to the side wall of the roller (28), the front end of the push rod (17) being rotatably connected to the rear end outer wall of the sealing plate (5), a fixing frame (16) being fixedly connected to the inner wall of the incubator (1) at the top end of the roller (28), and a stretching fixing assembly being arranged on the pull-out plate (3), the stretching fixing assembly being used to improve the stability of the pull-out plate (3) after the pull-out plate (3) is fully stretched from the inside of the incubator (1); The stretching and fixing assembly comprises a first slide rail (9), the first slide rail (9) being provided on the outer wall at the rear end of the sealing plate (5), a slider (8) being slidably connected inside the first slide rail (9), a telescopic rod (10) being rotatably connected to the slider (8), a fixing seat (12) being fixedly connected to the top end of the pull-out plate (3), an output end of the telescopic rod (10) being rotatably connected to the fixing seat (12), a first spring (11) for resetting the telescopic rod (10) being sleeved on the telescopic rod (10), and a limit support assembly being provided at the front end of the incubator (1), the limit support assembly being used to limit the pull-out plate (3) from sliding toward the front side of the incubator (1), and further providing support force for the pull-out plate (3) when the pull-out plate (3) slides toward the front side of the incubator (1); The position limiting support assembly comprises a connecting plate (21), the connecting plate (21) being fixed to the front outer wall of the incubator (1), a fixing frame (22) being arranged at the bottom end of the connecting plate (21), a cone plate (24) being slidably connected to the right end of the fixing frame (22), a second spring (23) for resetting the cone plate (24) being fixedly connected inside the fixing frame (22), a second sliding groove (18) for docking with the cone plate (24) being arranged on the left side wall of the pull-out plate (3), and a fixing frame (22) being fixedly connected at the top end thereof. A connecting seat (25) is provided, a swing rod (20) is arranged at the top of the fixing frame (22), the bottom end of the swing rod (20) is rotatably connected to the connecting seat (25), a slide rail is provided at the right end of the connecting plate (21), the top end of the swing rod (20) is slidably connected to the slide rail, the front end of the connecting plate (21) is rotatably connected to a limit plate (26), the swing rod (20) is provided with an opening for docking with the limit plate (26), and a third spring (27) for resetting the limit plate (26) is fixedly connected inside the connecting plate (21); Both ends of the top of the sealing plate (5) are respectively fixedly connected to a rotating shaft (7), and a groove rotatably connected to the rotating shaft (7) is provided inside the incubator (1).

2. The embryonic stem cell bionic culture system according to claim 1, characterized in that: A handle (4) is fixedly connected to the front end of the pull-out plate (3), and an anti-slip pad with a large friction coefficient is sleeved on the outside of the handle (4).

3. The embryonic stem cell bionic culture system according to claim 1, characterized in that: A plurality of equidistantly distributed pads (13) are fixedly connected to the top of the pull-out plate (3), and a plurality of equidistantly distributed air holes (14) are provided inside the pull-out plate (3).

4. The embryonic stem cell bionic culture system according to claim 1, characterized in that: The bottom of the front end of the incubator (1) is provided with a plurality of heat dissipation holes (2) distributed at equal intervals, and both sides of the interior of the sealing plate (5) are respectively fixedly connected with acrylic plates (6).

5. A bionic culture method for embryonic stem cells, applicable to any of the bionic culture systems for embryonic stem cells according to claims 1-4, characterized in that: The method comprises the following steps: Step 1: Stretch the drawer plate (3) outwards, and synchronously flip open the sealing plate (5) through the transmission support assembly; Step 2: Place the prepared cell culture dishes on top of the pull-out plate (3) in sequence; Step 3: Push the pull-out plate (3) back into the incubator (1), and the transmission support assembly will synchronously close the sealing plate (5); Step 4: Start the incubator (1) and set the corresponding ambient temperature to culture the cells.

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