Tumor tissue inoculation needle

Abstract

The present application discloses a tumor tissue inoculation needle, which relates to the technical field of inoculation needles, and comprises a main needle tube, a main needle head is arranged on the main needle tube, a main push plug is slidably arranged in the main needle tube, a push pin is slidably arranged in the main push plug, a flexible push head is arranged at the end of the push pin, a plurality of auxiliary needle tubes are arranged on the main needle tube, a movable sleeve is slidably arranged in the auxiliary needle tube, a push rod fixedly connected to the movable sleeve is slidably penetrated on the auxiliary needle tube, an auxiliary push plug is slidably arranged in the movable sleeve along its own axial direction, an auxiliary needle head is arranged at the bottom end of the auxiliary push plug, and the inner diameters of the plurality of auxiliary needle heads are different. The present application utilizes a plurality of auxiliary push plugs to store a plurality of tumor tissue pieces of different volumes, and uses a flexible push head and a plurality of auxiliary needle heads with different inner diameters to inoculate tumor tissue pieces of different volumes, so that tumor tissue pieces of different volumes can be continuously inoculated.

Description

Technical Field

[0001] The present application relates to the technical field of inoculation needles, and in particular to a tumor tissue inoculation needle. Background Art

[0002] When researching and developing anti-tumor drugs in the field of medicine, establishing an effective animal tumor transplantation model is the key to subsequent research. Among the various methods for establishing models, the small piece inoculation method is widely used due to its high tumor survival rate and the characteristics of promoting tumor volume growth.

[0003] The small piece inoculation method is to take out the tumor tissue from the pre-preserved animal, cut the tumor tissue into small pieces, and then inoculate it into the recipient animal. The tumor tissue is inoculated with an inoculation needle. First, the small piece of tumor tissue is sent into the inoculation needle, and then the needle is inserted into the recipient animal's body. Then the small piece of tumor tissue is pushed out of the inoculation needle and inoculated.

[0004] Each time a small piece of tumor tissue is loaded into the inoculation needle, it can only be inoculated once and cannot be continuously inoculated. Moreover, the inoculation needle can only inoculate a small piece of tumor tissue of a single volume. If the small piece of tumor tissue is too large, it is easy for the small piece of tumor tissue to get stuck in the needle. If the small piece of tumor tissue is too small, it is easy for the small piece of tumor tissue to slip out of the needle. Summary of the invention

[0005] In order to facilitate the continuous inoculation of small pieces of tumor tissue of different volumes, the present application provides a tumor tissue inoculation needle.

[0006] The tumor tissue inoculation needle provided in this application adopts the following technical solution:

[0007] A tumor tissue inoculation needle comprises a main needle tube, a main needle head is arranged at the end of the main needle tube, a main push plug is slidably arranged in the main needle tube, a push pin is slidably arranged in the main push plug, a plurality of auxiliary needle tubes connected to the main needle tube are arranged at intervals on the peripheral side wall of the main needle tube, a movable sleeve is slidably arranged in the auxiliary needle tube, a push rod fixedly connected to the movable sleeve is slidably penetrated on the auxiliary needle tube, a auxiliary push plug is slidably arranged in the movable sleeve along its own axial direction, a auxiliary push plug is slidably arranged in the bottom end of the auxiliary push plug, and the inner diameters of the plurality of auxiliary needle heads are different; a small piece of tumor tissue is placed in the auxiliary push plug, the push rod drives the movable sleeve to move below the main push plug, the main push plug is inserted in the auxiliary push plug and drives the auxiliary push plug to move, the auxiliary push plug drives the auxiliary needle head to move into the main needle head, and a flexible push head is arranged at the end of the push pin, the flexible push head moves into the auxiliary needle head and pushes the small piece of tumor tissue out of the auxiliary needle head.

[0008] By adopting the above technical scheme, when inoculating tumor tissue, multiple tumor tissue pieces of different volumes are placed in multiple auxiliary push plugs respectively, and the movable sleeve corresponding to the required inoculated tumor tissue piece is pushed into the main needle tube by the push rod, the main push plug is slid, and the main push plug pushes the auxiliary push plug to move downward in the movable sleeve, and the auxiliary push plug drives the auxiliary needle head to move into the main needle head, and then the push needle is slid, and the push needle drives the flexible push head to move into the auxiliary needle head, and the flexible push head pushes out the tumor tissue piece and inoculates it. After the inoculation is completed, the main push plug drives the auxiliary push plug to move upward and reset, and then the movable sleeve corresponding to the tumor tissue piece of another volume required to be inoculated is pushed into the main needle tube, and continuous inoculation can be performed, and the flexible push head has a certain deformation ability, so that it can enter the auxiliary needle heads with different inner diameters. In this way, multiple auxiliary push plugs are used to store multiple tumor tissue pieces of different volumes, and multiple auxiliary needle heads with different inner diameters are used to inoculate tumor tissue pieces of different volumes, so that tumor tissue pieces of different volumes can be inoculated continuously.

[0009] Preferably, a slider is provided on the outer wall of the auxiliary thrust plug, a movable groove is opened on the inner wall of the movable sleeve along its own axial direction, a locking groove is opened on the inner wall of the movable sleeve and located at the top end of the movable groove along its own circumferential direction, and the slider is slidably arranged in the movable groove and the locking groove.

[0010] By adopting the above technical solution, when the slider is located in the locking groove, the locking groove limits and fixes the auxiliary push plug along the axial direction of the movable sleeve. After the main push plug is moved and inserted into the auxiliary push plug, the main push plug is rotated, and the main push plug drives the slider to move from the locking groove into the movable groove through the auxiliary push plug. At this time, the locking of the auxiliary push plug is released, and the main push plug can drive the auxiliary push plug to move in the movable sleeve.

[0011] Preferably, an elastic sleeve is provided at one end of the secondary push plug close to the secondary needle, and the secondary needle is provided at one end of the elastic sleeve away from the secondary push plug. The elastic sleeve is tapered, and the diameter of the elastic sleeve gradually decreases from the secondary push plug to the secondary needle. The end of the main push plug moves into the elastic sleeve, and the main push plug squeezes the elastic sleeve and causes the elastic sleeve to deform, and the elastic sleeve is tightly fitted on the end of the main push plug.

[0012] By adopting the above technical solution, when the main push plug is slid toward the auxiliary push plug, the auxiliary push plug is limited and fixed in the movable sleeve. At this time, the elastic sleeve cannot move in the movable sleeve. The main push plug moves into the elastic sleeve and squeezes the elastic sleeve. The elastic sleeve is deformed and tightly sleeved on the main push plug, so that the auxiliary needle head and the auxiliary push plug can be fixed on the main push plug. The main push plug is rotated to release the limitation of the auxiliary push plug, and the main push plug can continue to slide toward the main needle head. When the tumor tissue block is inoculated, the main push plug is first moved away from the main needle head. The main push plug drives the auxiliary push plug and the auxiliary needle head to move and reset through the elastic sleeve. Then, the main push plug is rotated to make the slider enter the locking groove, and the auxiliary push plug can be limited and fixed again. Finally, the main push plug is continued to be moved away from the main needle head, so that the main push plug and the elastic sleeve can be separated, so that the auxiliary needle head can be reset, which is convenient for connection and inoculation.

[0013] Preferably, a cone head is formed at the end of the main push plug close to the elastic sleeve, and an inner convex ring is integrally formed on the inner wall of the elastic sleeve. The raised portion of the inner convex ring is located on the side close to the auxiliary push plug, the cone head has the same taper as that of the elastic sleeve, and the outer wall of the cone head is tightly pressed against the inner wall of the inner convex ring.

[0014] By adopting the above technical solution, when the main push plug enters the elastic sleeve, the inner convex ring is tightly sleeved on the cone head, so that the elastic sleeve is fixed on the main push plug more firmly. When the side wall of the cone head contacts the raised part of the inner convex ring, a closed cavity is formed between the cone head and the inner convex ring. As the cone head continues to move into the deformation sleeve, the air pressure in the sealed cavity gradually increases, and the small piece of tumor tissue can be pressed into the auxiliary needle head, so that when the main push plug is inserted into the elastic sleeve, the small piece of tumor tissue will not be squeezed, so that the small piece of tumor tissue is not easily damaged.

[0015] Preferably, a plurality of sealing rings are arranged at intervals inside the main push plug, the push pin slides through the plurality of sealing rings, and the inner side walls of the sealing rings abut against the outer side walls of the push pin.

[0016] By adopting the above technical solution, multiple sealing rings seal the gap between the main push plug and the push rod, thereby facilitating pressing the small piece of tumor tissue in the sealed chamber into the auxiliary needle.

[0017] Preferably, limiting grooves are provided on opposite sides of the inner wall of the auxiliary needle tube, limiting blocks are fixedly provided on opposite sides of the outer wall of the movable sleeve, and the two limiting blocks are slidably provided in the two limiting grooves along the sliding direction of the movable sleeve.

[0018] By adopting the above technical solution, the two limit blocks are driven to move in the two limit grooves during the sliding process of the moving sleeve, and the limit blocks cooperate with the limit grooves to limit and guide the moving sleeve, thereby improving the stability of the moving sleeve when sliding.

[0019] Preferably, four auxiliary needle tubes are provided, and the four auxiliary needle tubes are arranged at equal intervals along the circumference of the main needle tube. An abutment block is fixedly provided on one side of the side wall of the movable sleeve close to the middle of the main needle tube. The abutment block and the limit block are located in the same plane along the diameter direction of the movable sleeve. When a movable sleeve moves into the middle of the main needle tube, the abutment block and the two limit blocks on the movable sleeve respectively abut the abutment blocks on the other three movable sleeves.

[0020] By adopting the above technical solution, when a moving sleeve moves into the main needle tube, the abutment block and two limit blocks on the moving sleeve respectively abut the abutment blocks on the other three moving sleeves. With such a configuration, on the one hand, the abutment blocks on the other three moving sleeves position the moving sleeve through one abutment block and two limit blocks, so that the moving sleeve can accurately move to the middle of the main needle tube, and on the other hand, the moving sleeve abuts the abutment blocks on the other three moving sleeves through one abutment block and two limit blocks, so that the other three moving sleeves can be positioned, so that the other three moving sleeves will not move during the inoculation process.

[0021] Preferably, a first limit plate is provided on the push pin, and the first limit plate is located on a side of the main push plug away from the main needle tube.

[0022] By adopting the above technical solution, the push needle drives the first limit plate to move when it moves. When the first limit plate moves and abuts against the main push plug, the push needle drives the flexible push head to slide out of the auxiliary needle head, and the small piece of tumor tissue is pushed out of the auxiliary needle head by the flexible push head, thereby ensuring that the small piece of tumor tissue is inoculated into the body of the recipient animal.

[0023] Preferably, the top ends of the auxiliary needle tube and the auxiliary push plug are both opened, and a needle tube cover is detachably provided at the top open end of the auxiliary needle tube.

[0024] By adopting the above technical solution, the needle tube cover is removed from the auxiliary needle tube, and the small piece of tumor tissue can be placed into the auxiliary push plug from the opening end of the auxiliary needle tube and the auxiliary push plug, which is convenient for placing the small piece of tumor tissue. When the small piece of tumor tissue is placed, the needle tube cover is reinstalled on the auxiliary needle tube to seal the auxiliary needle tube, so that the small piece of tumor tissue is not easily contaminated.

[0025] Preferably, a mounting cap is detachably fixedly provided at the end of the push pin, and the flexible push head is fixedly provided on the mounting cap.

[0026] By adopting the above technical solution, after the tumor tissue fragment is inoculated, the mounting cap is removed from the push pin, and the flexible push head can be removed from the push pin, thereby facilitating the cleaning or replacement of the flexible push head.

[0027] In summary, the present application includes at least one of the following beneficial technical effects:

[0028] 1. Multiple auxiliary push plugs are used to store tumor tissue pieces of various sizes, and a flexible push head and multiple auxiliary needles with different inner diameters are used to inoculate tumor tissue pieces of various sizes, thereby enabling continuous inoculation of tumor tissue pieces of various sizes;

[0029] 2. With the help of the elastic sleeve and the slider, when the main push plug is slid toward the auxiliary push plug, the auxiliary push plug is limited and fixed in the movable sleeve by the slider and the limiting groove. At this time, the elastic sleeve cannot move in the movable sleeve. The main push plug moves into the elastic sleeve and squeezes the elastic sleeve. The elastic sleeve is deformed and tightly sleeved on the main push plug, so that the auxiliary needle head and the auxiliary push plug can be fixed on the main push plug. The main push plug is rotated to release the limitation of the auxiliary push plug, and the main push plug can continue to slide toward the main needle head. When the tumor tissue piece is inoculated, the main push plug is first moved away from the main needle head. The main push plug drives the auxiliary push plug and the auxiliary needle head to move and reset through the elastic sleeve. Then, the main push plug is rotated to make the slider enter the locking groove, and the auxiliary push plug can be limited and fixed again. Finally, the main push plug is continued to be moved away from the main needle head, so that the main push plug and the elastic sleeve can be separated, so that the auxiliary needle head can be reset, which is convenient for connection and inoculation.

[0030] 3. Through the limit blocks and the limit grooves, the two limit blocks are driven to move in the two limit grooves during the sliding process of the movable sleeve. The limit blocks and the limit grooves cooperate to limit and guide the movable sleeve, thereby improving the stability of the movable sleeve during sliding. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 This is a schematic diagram of the overall structure of the tumor tissue inoculation needle of this application;

[0032] Figure 2 This is a cross-sectional view of the overall structure of the tumor tissue inoculation needle of the present application;

[0033] Figure 3 The tumor tissue inoculation needle of the present application is an exploded cross-sectional view showing part of the structure of the moving groove;

[0034] Figure 4 The tumor tissue inoculation needle of the present application is a cross-sectional view highlighting the limiting groove;

[0035] Figure 5 For this application Figure 4 The enlarged schematic diagram at A in the middle;

[0036] Figure 6 For this application Figure 3 The enlarged schematic diagram of point B in the middle;

[0037] Figure 7 The tumor tissue inoculation needle of the present application is a partial structural cross-sectional view highlighting the state where the push head contacts the inner convex ring.

[0038] Figure numerals: 1. main needle tube; 2. main needle head; 3. main push plug; 4. push needle; 5. auxiliary needle tube; 6. movable sleeve; 7. push rod; 8. auxiliary push plug; 9. auxiliary needle head; 10. flexible push head; 11. slider; 12. movable groove; 13. locking groove; 14. elastic sleeve; 15. cone head; 16. inner convex ring; 17. sealing ring; 19. limiting groove; 20. limiting block; 21. abutment block; 22. first limiting plate; 23. needle tube cover; 24. mounting cap; 25. second limiting plate; 26. inner concave ring groove; 27. sealing chamber; 28. first guide inclined wall; 29. ​​second guide inclined wall. DETAILED DESCRIPTION

[0039] The following is combined with Figure 1-Figure 7 This application is described in further detail.

[0040] The embodiment of the present application discloses a tumor tissue inoculation needle.

[0041] Reference Figure 1 A tumor tissue inoculation needle comprises a main needle tube 1, a main needle head 2 is fixedly installed at the bottom end of the main needle tube 1, a main push plug 3 is slidably installed at the top end of the main needle tube 1 along its own axial direction, and a push needle 4 is slidably installed in the main push plug 3 along its own axial direction.

[0042] Reference Figure 1 and Figure 2 Four auxiliary needle tubes 5 are fixedly installed on the outer circumference of the main needle tube 1 at equal intervals along its own circumferential direction, and the inner cavity of the auxiliary needle tube 5 is connected with the inner cavity of the main needle tube 1. A movable sleeve 6 is installed in each auxiliary needle tube 5 along its own length direction, and the movable sleeve 6 is slidably installed in the main needle tube 1 and the auxiliary needle tube 5 along the diameter direction of the main needle tube 1.

[0043] Reference Figure 2 and Figure 3 The upper and lower ends of the movable sleeve 6 are open, and a hollow auxiliary push plug 8 is slidably installed in the movable sleeve 6 along its own axial direction. An elastic sleeve 14 is fixedly installed at the bottom end of the auxiliary push plug 8. The elastic sleeve 14 is conical, and the diameter of the top of the elastic sleeve 14 is larger than the diameter of the bottom end. A secondary needle 9 is fixedly installed at the bottom end of the elastic sleeve 14, and the inner diameters of the four secondary needles 9 are different. In the present application, the elastic sleeve 14 can be made of elastic materials such as rubber.

[0044] The top opening of the auxiliary needle tube 5 is provided, and a needle tube cover 23 is plugged and installed at the top opening of the auxiliary needle tube 5. The needle tube cover 23 is first removed from the auxiliary needle tube 5, and then tumor tissue pieces of different volumes are placed in different auxiliary push plugs 8, and the tumor tissue pieces fall at the bottom of the elastic sleeve 14, and the placement of the tumor tissue pieces is completed. After the tumor tissue pieces are placed, the needle tube cover 23 is reinstalled on the auxiliary needle tube 5, and the auxiliary needle tube 5 can be sealed, so that the tumor tissue pieces are not easily contaminated.

[0045] Reference Figure 4 and Figure 5 A push rod 7 is slidably installed in the middle of the side wall of the auxiliary needle tube 5 away from the main needle tube 1 along the diameter direction of the main needle tube 1. One end of the push rod 7 is fixedly connected to the movable sleeve 6, and the other end is located outside the auxiliary needle tube 5. The two opposite sides of the inner wall of the auxiliary needle tube 5 are provided with limiting grooves 19 along the diameter direction parallel to the main needle tube 1. The two opposite sides of the outer wall of the movable sleeve 6 are fixedly installed with limiting blocks 20, and the two limiting blocks 20 are slidably installed in the two limiting grooves 19. The movable sleeve 6 can be pushed from the auxiliary needle tube 5 into the main needle tube 1 by using the push rod 7. During the movement of the movable sleeve 6, the limiting block 20 slides in the limiting groove 19, thereby limiting and guiding the movable sleeve 6, so that the movement of the movable sleeve 6 is more stable.

[0046] An abutment block 21 is fixedly installed on the side wall of the mobile sleeve 6 close to the main needle tube 1, and the abutment block 21 is at the same height as the limit block 20. The mobile sleeve 6 corresponding to the tumor tissue to be inoculated is pushed from the auxiliary needle tube 5 into the main needle tube 1, and the mobile sleeve 6 drives one abutment block 21 and two limit blocks 20 to move synchronously. When the mobile sleeve 6 enters the middle of the main needle tube 1, one abutment block 21 and two limit blocks 20 respectively abut against the abutment blocks 21 on the other three mobile sleeves 6. In this way, on the one hand, the moving position of the mobile sleeve 6 can be positioned so that the mobile sleeve 6 can be moved to just below the main push plug 3; on the other hand, the other three mobile sleeves 6 can be positioned so that during the inoculation of small pieces of tumor tissue, the other three mobile sleeves 6 will not be displaced.

[0047] Reference Figure 3 and 6 Slide blocks 11 are fixedly installed on the opposite sides of the outer wall of the auxiliary thrust plug 8, and moving grooves 12 are opened on the opposite sides of the inner wall of the moving sleeve 6. The moving groove 12 is opened along the axial direction of the moving sleeve 6. The moving sleeve 6 is located at the top of the moving groove 12 and is provided with a locking groove 13 connected to the moving groove 12. The locking groove 13 is opened along the circumferential direction of the moving sleeve 6, and the slide blocks 11 are slidably installed in the moving groove 12 and the locking groove 13.

[0048] Reference Figure 2 , Figure 3 , Figure 6 and Figure 7When the secondary push plug 8 is located in the movable sleeve 6, the slider 11 on the secondary push plug 8 is located in the locking groove 13, and the locking groove 13 locks the secondary push plug 8 through the slider 11, so that the secondary push plug 8 cannot move downward along the axis direction of the movable sleeve 6. When inoculating, first push the movable sleeve 6 into the main needle tube 1, and then slide the main push plug 3 downward. The bottom end of the main push plug 3 is integrally formed with a cone head 15, the outer surface of the cone is a frosted surface, and the taper of the cone head 15 is the same as the taper of the elastic sleeve 14. An inner convex ring 16 is integrally formed on the inner side wall of the elastic sleeve 14, and the inner convex ring 16 protrudes toward the inside of the elastic sleeve 14, and the convex portion of the inner convex ring 16 is located on the side close to the secondary push plug 8.

[0049] The main push plug 3 drives the cone head 15 to move into the elastic sleeve 14. When the outer surface of the cone head 15 contacts the raised portion of the inner convex ring 16 (refer to Figure 7 ), the small piece of tumor tissue is located at the bottom of the inner convex ring 16 and the bottom end of the inner convex ring 16 is blocked. At this time, a closed cavity is formed between the inner convex ring 16 and the cone head 15. As the cone head 15 continues to move downward, the air pressure in the closed cavity gradually increases and presses the small piece of tumor tissue into the auxiliary needle head 9. As a result, when the main push plug 3 is inserted into the elastic sleeve 14, the small piece of tumor tissue will not be squeezed, so that the small piece of tumor tissue is not easily damaged. A sealing ring 17 is installed in the push head and the main push plug 3, and the push rod 7 slides through the sealing ring 17. The sealing ring 17 seals the gap between the push rod 7 and the main push plug 3, so that it is easy to press the small piece of tumor tissue in the sealed cavity 27 into the auxiliary needle head 9.

[0050] When the push head is fully inserted into the elastic sleeve 14 , the push head squeezes the inner convex ring 16 and the elastic sleeve 14 outward and causes them to deform. Under the action of the elastic force, the inner convex ring 16 tightly abuts against the frosted outer surface of the cone head 15 , thereby fixing the elastic sleeve 14 on the cone head 15 .

[0051] The end of the movable sleeve 6 located at the bottom wall of the locking groove 13 close to the movable groove 12 is inclined upward to form a second inclined guide wall 29, and the top of the side wall of the movable sleeve 6 located at the movable groove 12 away from the locking groove 13 is inclined upward toward the direction close to the locking groove 13 to form a first inclined guide wall 28. After the elastic sleeve 14 is fixedly sleeved on the push head, the main push plug 3 is rotated first, and the main push plug 3 drives the auxiliary push plug 8 to rotate through the cone head 15 and the elastic sleeve 14, and the auxiliary push plug 8 drives the slider 11 to slide in the locking groove 13, and the slider 11 passes through the second inclined guide wall 29 and the first inclined guide wall 28 and enters the movable groove 12. At this time, the main push plug 3 can continue to move downward, and the main push plug 3 drives the auxiliary push plug 8 to move downward in the movable sleeve 6, and the auxiliary push plug 8 drives the slider 11 to move downward in the movable groove 12.

[0052] The top of the main plunger 3 is integrally formed with a second stopper plate 25, and the bottom of the inner cavity of the movable sleeve 6 is formed with an inner concave annular groove 26. When the main plunger 3 drives the second stopper plate 25 to move and abut against the top of the main needle tube 1, the main plunger 3 drives the elastic sleeve 14 to move into the inner concave annular groove 26 through the cone head 15, and the elastic sleeve 14 drives the auxiliary needle 9 to move into the main needle 2.

[0053] The push needle 4 is threadedly detachably mounted with a mounting cap 24 at the bottom end near the main needle tube 1, and a flexible push head 10 is fixedly mounted on the mounting cap 24. The flexible push head 10 is conical, and the end with a smaller diameter of the flexible push head 10 faces the main needle tube 1. In the present application, the flexible push head 10 can be selected as a sponge.

[0054] The push pin 4 is pushed downward, and the push pin 4 drives the flexible push head 10 to move downward into the auxiliary needle head 9. The flexible push head 10 pushes the tumor tissue piece in the auxiliary needle head 9 into the body of the recipient animal, and the inoculation of the tumor tissue piece is completed. A first limit plate 22 is fixedly installed on the top of the push pin 4. When the push pin 4 moves and drives the first limit plate 22 to move and abut against the top of the main push plug 3, the push pin 4 drives the flexible push head 10 to slide out of the auxiliary needle head 9, and the tumor tissue piece is pushed out of the auxiliary needle head 9 by the flexible push head 10, thereby ensuring that the tumor tissue piece is inoculated in the body of the recipient animal.

[0055] After the tumor tissue piece is inoculated, the push needle 4 is first slid upward to make the flexible push head 10 move and reset, and then the main push plug 3 is slid upward. The main push plug 3 drives the auxiliary push plug 8 and the auxiliary needle head 9 to move upward through the elastic sleeve 14, and the auxiliary push plug 8 drives the slider 11 to move upward in the moving groove 12. When the slider 11 moves to the top of the moving groove 12, under the guidance of the first guide inclined wall 28, the slider 11 moves into the locking groove 13.

[0056] When the slider 11 enters the locking groove 13, the secondary push plug 8 is limited by the locking groove 13 and cannot move upward further. At this time, the main push plug 3 continues to move upward so that the push head is separated from the inner convex ring 16. The secondary push plug 8 drives the slider 11 downward under the action of gravity. The slider 11 enters the bottom of the locking groove 13 under the guidance of the second guide inclined wall 29, so that the secondary push plug 8 can be reset, and then the movable sleeve 6 is pulled by the push rod 7 to reset to the secondary needle tube 5.

[0057] When a tumor tissue piece is inoculated and the movable sleeve 6 is moved and reset to the auxiliary needle tube 5, the other movable sleeves 6 are pushed into the main needle tube 1 again, and tumor tissue pieces of different volumes can be inoculated, and the flexible push head 10 can be deformed and enter the auxiliary needle heads 9 of different inner diameters, so that tumor tissue pieces of different volumes can be continuously inoculated. After the tumor tissue piece is inoculated, the mounting cap 24 is removed from the push needle 4, and the flexible push head 10 can be removed from the push needle 4, so that the flexible push head 10 can be easily cleaned or replaced.

[0058] The implementation principle of a tumor tissue inoculation needle in the embodiment of the present application is as follows: when inoculating tumor tissue, multiple tumor tissue pieces of different volumes are respectively placed in multiple auxiliary push plugs 8, and the movable sleeve 6 corresponding to the required tumor tissue piece to be inoculated is pushed into the main needle tube 1 by the push rod 7, and the main push plug 3 is slid, and the main push plug 3 pushes the auxiliary push plug 8 to move downward in the movable sleeve 6, and the auxiliary push plug 8 drives the auxiliary needle head 9 to move into the main needle head 2, and then the push needle 4 is slid, and the push needle 4 drives the flexible push head 10 to move into the auxiliary needle head 9, and the flexible push head 10 pushes out the tumor tissue piece and inoculates it. After the inoculation is completed, the main push plug 3 drives the auxiliary push plug 8 to move upward and reset, and then the movable sleeve 6 corresponding to the tumor tissue piece of another volume to be inoculated is pushed into the main needle tube 1, and continuous inoculation can be performed, and the flexible push head 10 has a certain deformation ability, so that it can enter the auxiliary needle heads 9 with different inner diameters. In this way, multiple auxiliary plungers 8 are used to store tumor tissue pieces of multiple different volumes, and multiple auxiliary needles 9 with different inner diameters are used to inoculate tumor tissue pieces of different volumes, so that tumor tissue pieces of different volumes can be inoculated continuously.

[0059] The above are all preferred embodiments of the present application, and the protection scope of the present application is not limited thereto. Therefore, any equivalent changes made according to the structure, shape, and principle of the present application should be included in the protection scope of the present application.

Claims

1. A tumor tissue inoculation needle, comprising a main needle tube (1), a main needle head (2) being arranged at the end of the main needle tube (1), a main push plug (3) being slidably arranged in the main needle tube (1), and a push needle (4) being slidably arranged in the main push plug (3), wherein: A plurality of auxiliary needle tubes (5) connected to the main needle tube (1) are arranged at intervals on the peripheral side wall of the main needle tube (1); a movable sleeve (6) is slidably arranged in the auxiliary needle tube (5); a push rod (7) fixedly connected to the movable sleeve (6) is slidably penetrated through the auxiliary needle tube (5); an auxiliary push plug (8) is slidably arranged in the movable sleeve (6) along its own axial direction; an auxiliary needle head (9) is arranged at the bottom end of the auxiliary push plug (8), and the inner diameters of the plurality of auxiliary needle heads (9) are different; a small piece of tumor tissue is placed in the auxiliary push plug (8), and the push rod (7) is inserted into the auxiliary needle plug (8) to slide along the axial direction of the auxiliary push plug (6); ) drives the movable sleeve (6) to move to the bottom of the main push plug (3), the main push plug (3) is inserted into the auxiliary push plug (8) and drives the auxiliary push plug (8) to move, the auxiliary push plug (8) drives the auxiliary needle (9) to move into the main needle (2), the end of the push needle (4) is provided with a flexible push head (10), the flexible push head (10) moves into the auxiliary needle (9) and pushes the tumor tissue piece out of the auxiliary needle (9); the outer wall of the auxiliary push plug (8) is provided with a slider (11), and the inner wall of the movable sleeve (6) is opened along its own axis direction. A movable groove (12) is provided, a locking groove (13) is provided on the inner side wall of the movable sleeve (6) and at the top end of the movable groove (12) along its own circumferential direction, the sliding block (11) is slidably arranged in the movable groove (12) and the locking groove (13), limiting grooves (19) are provided on opposite sides of the inner side wall of the auxiliary needle tube (5), limiting blocks (20) are fixedly arranged on opposite sides of the outer side wall of the movable sleeve (6), and the two limiting blocks (20) are slidably arranged in the two limiting grooves (19) along the sliding direction of the movable sleeve (6), and the auxiliary needle tube (5) is provided with a movable groove (12) and a locking groove (13). Four needle tubes (5) are provided, and the four auxiliary needle tubes (5) are arranged at equal intervals along the circumference of the main needle tube (1). A contact block (21) is fixedly provided on one side of the side wall of the movable sleeve (6) close to the middle of the main needle tube (1). The contact block (21) and the limit block (20) are located in the same plane along the diameter direction of the movable sleeve (6). When one movable sleeve (6) moves into the middle of the main needle tube (1), the contact block (21) and the two limit blocks (20) on the movable sleeve (6) respectively contact the contact blocks (21) on the other three movable sleeves (6).

2. A tumor tissue inoculation needle according to claim 1, characterized in that: An elastic sleeve (14) is arranged at one end of the secondary push plug (8) close to the secondary needle head (9), and the secondary needle head (9) is arranged at one end of the elastic sleeve (14) away from the secondary push plug (8). The elastic sleeve (14) is tapered, and the diameter of the elastic sleeve (14) gradually decreases from the secondary push plug (8) to the secondary needle head (9). The end of the main push plug (3) moves into the elastic sleeve (14), and the main push plug (3) squeezes the elastic sleeve (14) and causes the elastic sleeve (14) to deform. The elastic sleeve (14) is tightly sleeved on the end of the main push plug (3).

3. A tumor tissue inoculation needle according to claim 2, characterized in that: The main thrust plug (3) is formed with a cone head (15) at the end close to the elastic sleeve (14), and an inner convex ring (16) is integrally formed on the inner side wall of the elastic sleeve (14). The convex portion of the inner convex ring (16) is located on the side close to the auxiliary thrust plug (8). The cone head (15) has the same taper as the elastic sleeve (14), and the outer side wall of the cone head (15) is tightly pressed against the inner side wall of the inner convex ring (16).

4. A tumor tissue inoculation needle according to claim 3, characterized in that: A plurality of sealing rings (17) are arranged at intervals inside the main push plug (3), the push pin (4) slides through the plurality of sealing rings (17), and the inner side wall of the sealing ring (17) abuts against the outer side wall of the push pin (4).

5. The tumor tissue inoculation needle according to claim 1, characterized in that: The push needle (4) is provided with a first limit plate (22), and the first limit plate (22) is located on a side of the main push plug (3) away from the main needle tube (1).

6. A tumor tissue inoculation needle according to claim 1, characterized in that: The top ends of the auxiliary needle tube (5) and the auxiliary push plug (8) are both opened, and a needle tube cover (23) is detachably provided at the top open end of the auxiliary needle tube (5).

7. The tumor tissue inoculation needle according to claim 1, characterized in that: The end of the push pin (4) is detachably and fixedly provided with a mounting cap (24), and the flexible push head (10) is fixedly provided on the mounting cap (24).

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