Acidulant complex containing fumaric acid and use thereof

Abstract

The invention discloses an acidulant complex containing fumaric acid and its use, belongs to the technical field of acidulant complexes, and specifically relates to an acidulant complex, including: at least containing fumaric acid, and an adjuvant; the content of fumaric acid is more than 50wt%, and the adjuvant includes at least one of formic acid, benzoic acid, citric acid, phosphoric acid, lactic acid, formic acid-ammonium formate, dextrin, potassium diformate and trehalose ester, and the trehalose ester has a fructose acid group. The present invention adopts an acidulant complex containing fumaric acid as the main component, which can be used in feed, can promote the growth of poultry and livestock, and reduce pathogen infection, enhance body immunity and animal anti-stress ability.

Description

Technical Field

[0001] The invention belongs to the technical field of acidulant complexes, and in particular relates to an acidulant complex containing fumaric acid and a use thereof. Background Art

[0002] Acidifier is a feed additive that can reduce the pH value of feed in the digestive tract. There are three main types of acidifiers currently used: inorganic acidifiers, organic acidifiers and compound acidifiers. Inorganic acidifiers generally include phosphoric acid, hydrochloric acid and sulfuric acid; common organic acidifiers include formic acid, acetic acid, propionic acid, butyric acid, sorbic acid, fumaric acid, citric acid, etc.; compound acidifiers refer to the combination of inorganic acid and organic acid or organic acid and organic acid to enhance the effect of acidifiers. Acidifiers are fed to animals in combination with diets to increase the acidity of the diet and regulate the pH value in the gastrointestinal tract of animals, thereby reducing pathogen infection, enhancing the body's immune function and the animal's anti-stress ability, and improving animal production performance.

[0003] Most of the single acidifiers and compound acidifiers put into production in the prior art are made from purified chemical raw materials alone or mixed in a certain proportion. There are many problems with feed acidifiers, such as incomplete substrate utilization and weak growth-promoting effects. The development of new feed additives still has great potential development value. Summary of the invention

[0004] The purpose of the present invention is to provide an acidifier compound containing fumaric acid and its use which can promote the growth of poultry and livestock, reduce pathogen infection, enhance the body's immune function and the animal's anti-stress ability.

[0005] The technical solution adopted by the present invention to achieve the above-mentioned purpose is:

[0006] An acidulant complex comprises: at least fumaric acid and an adjuvant; the content of fumaric acid is more than 50wt%, the adjuvant comprises at least one of formic acid, benzoic acid, citric acid, phosphoric acid, lactic acid, formic acid-ammonium formate, dextrin, potassium diformate and trehalose ester, and the trehalose ester has a fructose acid group. The present invention mainly uses fumaric acid and adjuvants to prepare an acidulant complex. The acidulant complex prepared in the present invention can be used in feed. The present invention finds that fumaric acid can increase the acidity of the diet, regulate the pH value in the gastrointestinal tract of animals, thereby reducing pathogen infection, enhancing the body's immune performance and animal anti-stress ability, and improving animal production performance. When it is compounded with at least one of the adjuvants, the obtained compound can further reduce pathogen infection, enhance the body's immune performance and animal anti-stress ability, and promote the growth of poultry and livestock.

[0007] Preferably, fumaric acid is mixed with an adjuvant to form an acidulant complex; or, the content of fumaric acid is less than 96wt%; or, the content of fumaric acid is any one of 55wt%, 60wt%, 65wt%, 70wt%, 75wt%, 80wt%, 85wt% and 90wt%.

[0008] Preferably, fumaric acid is mixed with an adjuvant to form an acidulant complex; or, the content of the adjuvant is less than 50 wt %; or, the content of the adjuvant is greater than 4.76 wt %.

[0009] Preferably, the trehalose ester is prepared by reacting fructose and trehalose; or, in the preparation of trehalose ester, fructose and trehalose are prepared into trehalose ester under the action of TBTU; or, in the preparation of trehalose ester, fructose and trehalose are reacted in anhydrous pyridine containing TBTU to prepare trehalose ester. When the trehalose ester in the present invention is used in a small amount, it cannot promote the growth of poultry and livestock, but under the use of fumaric acid, the acidulant complex containing trehalose ester has a better effect of promoting the growth of poultry and livestock, which is better than the acidulant complex without trehalose ester.

[0010] Preferably, in the preparation of trehalose ester, fructose acid and TBTU are added to anhydrous pyridine, stirred and mixed under the protection of inert gas, and then trehalose is added and stirred and mixed, and the mixture is reacted at 20-40° C. for 24-72 hours. After the reaction is completed, the mixture is separated by column chromatography and concentrated to obtain trehalose ester.

[0011] More preferably, in the preparation of trehalose ester, the amount of fructic acid used is 0.5-3wt% of anhydrous pyridine.

[0012] More preferably, in the preparation of trehalose ester, the amount of TBTU used is 150-250 wt % of fructic acid.

[0013] More preferably, in the preparation of trehalose ester, the amount of trehalose used is 100-400 wt % of fructose acid.

[0014] More preferably, in the preparation of alginate ester, dichloromethane and methanol are used as eluents in column chromatography separation, and the dichloromethane and methanol in the eluent are mixed in a volume ratio of 1:0.5-3.

[0015] Preferably, the acidulant complex comprises fumaric acid and an adjuvant.

[0016] Preferably, the auxiliary agent includes at least one of an acid agent, a formic acid-containing agent, and a non-acidic carbohydrate agent.

[0017] Preferably, the acidic agent includes at least one of formic acid, benzoic acid, citric acid, phosphoric acid and lactic acid.

[0018] Preferably, the formic acid-containing reagent comprises at least one of formic acid-ammonium formate, succinic acid and potassium diformate.

[0019] Preferably, the non-acid carbohydrate comprises at least one of trehalose ester and glabrigenin.

[0020] More preferably, the amount of the auxiliary agent used is 5 wt % or more of the fumaric acid.

[0021] More preferably, the amount of the auxiliary agent used is less than 100 wt % of the fumaric acid.

[0022] More preferably, the amount of the acid agent used is 5 wt % or more of the fumaric acid.

[0023] More preferably, the amount of the acid agent used is less than 100 wt % of the fumaric acid.

[0024] More preferably, the amount of formic acid used is 5-40 wt % of the fumaric acid.

[0025] More preferably, the amount of benzoic acid used is 5-40 wt % of fumaric acid.

[0026] More preferably, the amount of citric acid used is 5-40 wt % of the fumaric acid.

[0027] More preferably, the amount of phosphoric acid used is 5-40 wt % of fumaric acid.

[0028] More preferably, the amount of lactic acid used is 5-40 wt % of fumaric acid.

[0029] More preferably, the usage amount of the formic acid-containing reagent is 5% or more.

[0030] More preferably, the usage amount of the formic acid-containing reagent is 100% or less.

[0031] More preferably, the amount of formic acid-ammonium formate used is 5-40 wt % of fumaric acid.

[0032] More preferably, the amount of dapoxetine used is 5-40 wt % of fumaric acid.

[0033] More preferably, the amount of potassium diformate used is 5-40wt% of the fumaric acid.

[0034] More preferably, the amount of non-acid carbohydrate used is 5% or more.

[0035] More preferably, the amount of non-acid carbohydrate used is less than 100%.

[0036] More preferably, the amount of alginate used is 5-40 wt % of fumaric acid.

[0037] More preferably, the amount of glabrigenin used is 5-40wt% of fumaric acid. When used in a small amount, the glabrigenin in the present invention cannot promote the growth of livestock, but under the use of fumaric acid, the acidulant complex containing glabrigenin has a better effect of promoting the growth of livestock, which is better than the acidulant complex without glabrigenin. Further, glabrigenin can also be used together with trehalose ester in the acidulant complex, and has excellent use effect when used in a small amount.

[0038] The invention discloses a feed, comprising: the above-mentioned acidifier complex.

[0039] Preferably, the feed is livestock and poultry breeding feed; or, the feed is pig breeding feed; or, the feed is chicken breeding feed.

[0040] Preferably, the acidifier complex is used in an amount of 0.025-3 wt % of the feed.

[0041] The fortified feed comprises feed and an acidifier compound, wherein the content of the acidifier compound in the fortified feed is 0.05-2wt%.

[0042] The invention discloses the use of the acidifier complex in preparing antibacterial products and / or anti-Escherichia coli products and / or anti-Staphylococcus aureus products and / or feed products.

[0043] The present invention adopts an acidifier compound containing fumaric acid as the main component, and is matched with an adjuvant, wherein the adjuvant can be selected from at least one of formic acid, benzoic acid, citric acid, phosphoric acid, lactic acid, formic acid-ammonium formate, dextrin, potassium diformate and trehalose ester, so that the present invention has the following beneficial effects: the present invention can be used in feed, can promote the growth of poultry and livestock, reduce pathogenic bacteria infection, enhance the body's immune performance and the animal's anti-stress ability. Therefore, the present invention is an acidifier compound containing fumaric acid, which can promote the growth of poultry and livestock, reduce pathogenic bacteria infection, enhance the body's immune performance and the animal's anti-stress ability, and its use. BRIEF DESCRIPTION OF THE DRAWINGS

[0044] Figure 1 It is an infrared spectrum.

[0045] Figure 2 This is a graph of average daily weight gain.

[0046] Figure 3 This is a graph showing the urea nitrogen content in serum. DETAILED DESCRIPTION

[0047] The technical solution of the present invention is further described in detail below in conjunction with specific implementations and drawings:

[0048] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0049] The following first describes the concepts involved in the present application in conjunction with the accompanying drawings. It should be noted that the following description of each concept is only to make the content of the present application easier to understand, and does not limit the scope of protection of the present application; at the same time, the embodiments and features in the embodiments of the present application can be combined with each other in the absence of conflict. The present application will be described in detail with reference to the accompanying drawings and in conjunction with the embodiments.

[0050] Example 1: A fumaric acid-containing acidulant complex

[0051] Acidifier complex: fumaric acid and formic acid-ammonium formate. The content of formic acid-ammonium formate is 10wt% of fumaric acid, and formic acid-ammonium formate is composed of formic acid and ammonium formate, and formic acid-ammonium formate contains 50wt% of formic acid.

[0052] Example 2: A fumaric acid-containing acidulant complex

[0053] Acidifier complex: fumaric acid and hygroscopic acid. The content of hygroscopic acid is 10wt% of fumaric acid, the main component of hygroscopic acid is formic acid, and hygroscopic acid is purchased from Changsha Bosheng Biotechnology Co., Ltd.

[0054] Example 3: A fumaric acid-containing acidulant complex

[0055] Acidifier complex: fumaric acid and potassium diformate. The content of potassium diformate is 10wt% of fumaric acid.

[0056] Example 4: A fumaric acid-containing acidulant complex

[0057] Acidifier complex: fumaric acid and formic acid. The content of formic acid is 10wt% of fumaric acid.

[0058] Example 5: A fumaric acid-containing acidulant complex

[0059] Acidifier complex: fumaric acid and benzoic acid. The content of benzoic acid is 10wt% of fumaric acid.

[0060] Example 6: A fumaric acid-containing acidulant complex

[0061] Acidifier complex: fumaric acid and citric acid. The content of citric acid is 10wt% of fumaric acid.

[0062] Example 7: A fumaric acid-containing acidulant complex

[0063] Acidifier complex: fumaric acid and phosphoric acid. The content of phosphoric acid is 10wt% of fumaric acid.

[0064] Example 8: A fumaric acid-containing acidulant complex

[0065] Acidifier complex: fumaric acid and lactic acid. The content of lactic acid is 10wt% of fumaric acid.

[0066] Example 9: A fumaric acid-containing acidulant complex

[0067] Acidifier complex: fumaric acid and alginate. The content of alginate is 10wt% of fumaric acid.

[0068] Preparation of trehalose ester: Add fructose acid and TBTU to anhydrous pyridine, stir and mix under the protection of inert gas, then add trehalose and stir and mix, react at 30°C for 48 hours, and after the reaction is completed, separate by column chromatography and concentrate to obtain trehalose ester. The amount of fructose acid used is 1.5wt% of anhydrous pyridine, the amount of TBTU used is 200wt% of fructose acid, and the amount of trehalose used is 300wt% of fructose acid. In column chromatography separation, dichloromethane and methanol are used as eluents, and dichloromethane and methanol are mixed in the eluent at a volume ratio of 1:1.

[0069] Example 10: A fumaric acid-containing acidulant complex

[0070] Acidifier complex: fumaric acid and alginate. The content of alginate is 30wt% of fumaric acid. The preparation method of alginate is the same as that of Example 9.

[0071] Example 11: A fumaric acid-containing acidulant complex

[0072] Acidifier complex: fumaric acid and glabrigenin. The content of glabrigenin is 10wt% of fumaric acid.

[0073] Example 12: A Fumaric Acid-Containing Acidulant Complex

[0074] Acidifier complex: fumaric acid and glabrigenin. The content of glabrigenin is 30wt% of fumaric acid.

[0075] Example 13: A Fumaric Acid-Containing Acidulant Complex

[0076] Acidifier complex: fumaric acid, lactic acid, potassium diformate. The content of lactic acid is 10wt% of fumaric acid, and the content of potassium diformate is 10wt% of fumaric acid.

[0077] Example 14: A Fumaric Acid-Containing Acidulant Complex

[0078] Acidifier complex: fumaric acid, lactic acid and citric acid. The content of lactic acid is 10wt% of the fumaric acid, and the content of citric acid is 10wt% of the fumaric acid.

[0079] Example 15: A Fumaric Acid-Containing Acidulant Complex

[0080] Acidifier complex: fumaric acid and lactic acid, formic acid-ammonium formate. The content of lactic acid is 10wt% of fumaric acid, the content of formic acid-ammonium formate is 10wt% of fumaric acid, formic acid-ammonium formate is composed of formic acid and ammonium formate, and formic acid-ammonium formate contains 50wt% of formic acid.

[0081] Example 16: A Fumaric Acid-Containing Acidulant Complex

[0082] Acidifier complex: fumaric acid, lactic acid, and trehalose ester. The content of lactic acid is 10wt% of fumaric acid, and the content of trehalose ester is 10wt% of fumaric acid. The preparation method of trehalose ester is the same as that of Example 9.

[0083] Example 17: A Fumaric Acid-Containing Acidulant Complex

[0084] Acidifier complex: fumaric acid, lactic acid, and glabrigenin. The content of lactic acid is 10wt% of the fumaric acid, and the content of glabrigenin is 10wt% of the fumaric acid.

[0085] Example 18: A Fumaric Acid-Containing Acidulant Complex

[0086] Acidifier complex: fumaric acid, trehalose ester, and glabrigenin. The content of trehalose ester is 10wt% of fumaric acid, and the content of glabrigenin is 10wt% of fumaric acid. The preparation method of trehalose ester is the same as that of Example 9.

[0087] Example 19: A Fumaric Acid-Containing Acidulant Complex

[0088] Acidifier complex: fumaric acid, lactic acid, citric acid, potassium diformate. The content of lactic acid is 10wt% of fumaric acid, the content of citric acid is 10wt% of fumaric acid, and the content of potassium diformate is 10wt% of fumaric acid.

[0089] Example 20: A Fumaric Acid-Containing Acidulant Complex

[0090] Acidifier complex: fumaric acid, lactic acid, citric acid, and trehalose ester. The content of lactic acid is 10 wt % of fumaric acid, the content of citric acid is 10 wt % of fumaric acid, and the content of trehalose ester is 10 wt % of fumaric acid. The preparation method of trehalose ester is the same as that of Example 9.

[0091] Example 21: A Fumaric Acid-Containing Acidulant Complex

[0092] Acidifier complex: fumaric acid, lactic acid, citric acid, glabrigenin. The content of lactic acid is 10wt% of fumaric acid, the content of citric acid is 10wt% of fumaric acid, and the content of glabrigenin is 10wt% of fumaric acid.

[0093] Example 22: A Fumaric Acid-Containing Acidulant Complex

[0094] Acidifier complex: fumaric acid and lactic acid, trehalose ester, glabrigenin. The content of lactic acid is 10wt% of fumaric acid, the content of trehalose ester is 10wt% of fumaric acid, and the content of glabrigenin is 10wt% of fumaric acid. The preparation method of trehalose ester is the same as that of Example 9.

[0095] Example 23: A Fumaric Acid-Containing Acidulant Complex

[0096] Acidifier complex: fumaric acid, citric acid, trehalose ester, glabrigenin. The content of citric acid is 10wt% of fumaric acid, the content of trehalose ester is 10wt% of fumaric acid, and the content of glabrigenin is 10wt% of fumaric acid. The preparation method of trehalose ester is the same as that of Example 9.

[0097] Example 24: A Fumaric Acid-Containing Acidulant Complex

[0098] Acidifier complex: fumaric acid and lactic acid, citric acid, potassium diformate, trehalose ester. The content of lactic acid is 10wt% of fumaric acid, the content of citric acid is 10wt% of fumaric acid, the content of potassium diformate is 10wt% of fumaric acid, and the content of trehalose ester is 10wt% of fumaric acid. The preparation of trehalose ester is the same as Example 9.

[0099] Example 25: A Fumaric Acid-Containing Acidulant Complex

[0100] Acidifier complex: fumaric acid and lactic acid, citric acid, potassium diformate, trehalose ester, glabrigenin. The content of lactic acid is 10wt% of fumaric acid, the content of citric acid is 10wt% of fumaric acid, the content of potassium diformate is 10wt% of fumaric acid, the content of trehalose ester is 10wt% of fumaric acid, and the content of glabrigenin is 10wt% of fumaric acid. The preparation of trehalose ester is the same as that of Example 9.

[0101] Comparative Example 1:

[0102] Single acidifier: fumaric acid.

[0103] Comparative Example 2:

[0104] Single acidifier: formic acid.

[0105] Comparative Example 3:

[0106] Single acidifier: benzoic acid.

[0107] Comparative Example 4:

[0108] Single acidifier: citric acid.

[0109] Comparative Example 5:

[0110] Single acidifier: phosphoric acid.

[0111] Comparative Example 6:

[0112] Single acidifier: lactic acid.

[0113] Comparative Example 7:

[0114] Composite acidifier: formic acid-ammonium formate.

[0115] Comparative Example 8:

[0116] Composite acidifier: Soft acid.

[0117] Comparative Example 9:

[0118] Single acidifier: potassium diformate.

[0119] Comparative Example 10:

[0120] Single reagent: trehalose ester. The preparation method of trehalose ester is the same as that of Example 9.

[0121] Comparative Example 11:

[0122] Single agent: glabrigenin.

[0123] Example 26: A fortified feed

[0124] Fortified feed: Add the fortifier to the basic feed and mix to obtain fortified feed. The amount of the fortifier used is 1wt% of the feed. The fortifier is the acidifier complex prepared in Example 1. The basic feed contains 75.35wt% corn, 13.15wt% wheat bran, 2wt% rice bran, 7wt% soybean meal, 0.6wt% calcium hydrogen phosphate, 0.9wt% choline chloride, 0.3wt% lysine, 0.2wt% sodium chloride, 0.1wt% sodium bicarbonate, and the remainder is premix. The premix provides per kilogram of feed: Vitamin A 12400IU, Vitamin D3 2800IU, Vitamin E 90mg, Vitamin K 38mg, Vitamin B1 3mg, Vitamin B2 10mg, Vitamin B 1230μg, biotin 0.4mg, pantothenic acid 14mg, folic acid 0.6mg, niacin 30mg, Fe (FeSO4·H2O) 80mg, Zn (ZnSO4·H2O) 70mg, Cu (CuSO4·5H2O) 20mg, Mn (MnSO4·H2O) 20mg, Se (Na2SeO3) 0.3mg, I (CaI2O6) 0.3mg.

[0125] In the present invention, the fortifier in Example 26 can be replaced by any product in Examples 2-25 of the present invention or any product in Comparative Examples 1-11. The basic feed can be commercially available feed.

[0126] In the present invention, the enhancer in Example 26 may also be replaced by a combination of multiple acidifiers or a single agent or a combination of an acidifier and a single agent within the scope of the present invention.

[0127] Test example:

[0128] 1. Infrared detection

[0129] The present invention conducted infrared characterization on the trehalose ester prepared in Example 9, and the results are as follows: Figure 1 As shown, at 3458 cm -1 The infrared absorption peak of hydroxyl is at 2963 cm -1 and 2846 cm -1 The infrared absorption peak of carbon and hydrogen is at 1731 cm -1 The infrared absorption peak of the carbon-oxygen double bond is at 1043 cm -1 The infrared absorption peak of carbon and oxygen is at , indicating that alginate ester is obtained.

[0130] 2 Antibacterial test

[0131] The present invention weighs the fumaric acid, formic acid, benzoic acid, citric acid, phosphoric acid, lactic acid, formic acid-ammonium formate, dapoxic acid, potassium diformate, trehalose ester and glabrigenin samples in comparative examples 1-11 respectively, and prepares them into required concentrations respectively. The fumaric acid, benzoic acid, trehalose ester and glabrigenin are dissolved with anhydrous ethanol, and the citric acid, formic acid, lactic acid, phosphoric acid, formic acid-ammonium formate, dapoxic acid and potassium diformate are diluted with water.

[0132] The Escherichia coli ATCC25922 used in the present invention was purchased from Guangzhou Huankai Biotechnology Co., Ltd., and Staphylococcus aureus ATCC6538 was purchased from Guangzhou Huankai Biotechnology Co., Ltd. According to the instructions for Escherichia coli and Staphylococcus aureus, the lyophilized powder was diluted, and the bacterial solution was taken after dilution to apply to the plate, and it was grown for about 24 hours, and a single colony was picked for culture, and then stored after culture. Prepare LB solution, take 5 mL LB solution, and set up a control group; treat it by 2-fold dilution method, the concentrations are 10, 5, 2.5, 1.25, 0.625, and 0.3125 mg / mL respectively, add 5 mL LB solution to the control group, add 0.1 mL bacterial solution to each test tube, mix well, observe after culture, and in the order of marking, the reagent concentration in the last clear test tube is the minimum inhibitory concentration (MIC).

[0133] Table 1 Minimum inhibitory concentration (MIC)

[0134]

[0135] As shown in Table 1, formic acid has the best antibacterial effect on Escherichia coli, with a concentration of 0.625 mg / mL, followed by benzoic acid, with a minimum inhibitory concentration of 1.25 mg / mL. The minimum inhibitory concentrations of formic acid and benzoic acid on Staphylococcus aureus are 1.25 mg / mL, the minimum inhibitory concentrations of fumaric acid on Escherichia coli and Staphylococcus aureus are 1.5 mg / ml, the minimum inhibitory concentrations of citric acid on Escherichia coli and Staphylococcus aureus are both 2.0 mg / ml, the minimum inhibitory concentrations of phosphoric acid on Escherichia coli and Staphylococcus aureus are both 2.0 mg / ml, and the minimum inhibitory concentrations of lactic acid on Escherichia coli and Staphylococcus aureus are both 2.0 mg / ml. The inhibitory concentrations of doxycycline and potassium diformate against E. coli and Staphylococcus aureus were both 2.5 mg / ml; the minimum inhibitory concentration of formic acid-ammonium formate against E. coli was 0.625 mg / ml, and the minimum inhibitory concentration against Staphylococcus aureus was 1.25 mg / ml. In the minimum inhibitory concentration experiment, benzoic acid and formic acid had the lowest minimum inhibitory concentration against E. coli and Staphylococcus aureus, followed by fumaric acid, and then phosphoric acid and lactic acid, with the minimum inhibitory concentrations against E. coli and Staphylococcus aureus being 2 mg / ml, and the minimum inhibitory concentration of citric acid against E. coli and Staphylococcus aureus was 2.5 mg / ml. The solubility of benzoic acid and fumaric acid in water is very small, so in the determination of the minimum inhibitory concentration, benzoic acid is insoluble in water at 10 mg / mL and exists in solid form. At this concentration, both Escherichia coli and Staphylococcus aureus grow. That is to say, in in vitro experiments, only when the acidifier is dissolved in the solution can it have an antibacterial effect. The acidifier that exists in the solution as a solid has no antibacterial effect. Trehalose ester and glabrigenin have no antibacterial effect.

[0136] 3. Growth promotion test

[0137] Healthy and evenly sized fragrant piglets weighing about 25kg were selected and fed with basic feed or fortified feed. They had drinking water by themselves during the breeding period and were fed at 9:00 and 17:00 every day. Disinfection and cleaning were carried out according to routine procedures during the breeding period. Ventilation and cleanliness were maintained and a breeding experiment was carried out for 60 days.

[0138] The basic feed for the growth promotion test in the present invention is the same as the basic feed in Example 26, and the fortified feed is a fortified feed prepared according to the method of Example 26 and added with different fortifiers.

[0139] The weight of the piglets before and after breeding was weighed and the average daily weight gain was calculated. Figure 2As shown, SL1 is the fortified feed prepared in Example 9, SL2 is the fortified feed prepared in Example 10, SL3 is the fortified feed prepared in Example 11, SL4 is the fortified feed prepared in Example 12, SL5 is the fortified feed prepared in Example 16, SL6 is the fortified feed prepared in Example 17, SL7 is the fortified feed prepared in Example 18, SL8 is the fortified feed prepared in Comparative Example 1, SL9 is the fortified feed prepared in Comparative Example 6, wherein the amount of lactic acid used is the same as the actual amount of lactic acid used in Example 16, and SL10 is the fortified feed prepared in Comparative Example 10 The prepared fortified feed, wherein the amount of trehalose ester used is the same as the actual amount of trehalose ester used in Example 9, SL11 is the fortified feed prepared in Comparative Example 11, wherein the amount of glabrigenin used is the same as the actual amount of glabrigenin used in Example 11, and the fortified feeds in the present invention have different effects on the average daily weight gain of fragrant piglets. The fortified feed in the present invention needs to be added with a fortifier, and the fortifier must contain fumaric acid. After using fumaric acid, other adjuvants with a smaller amount of fumaric acid can also be added. The adjuvant can use one or more of the acidic agents such as formic acid, benzoic acid, citric acid, phosphoric acid and lactic acid, and can also One or more of the formic acid-containing agents such as formic acid-ammonium formate, succinic acid and potassium diformate can be used, and one or two of the non-acidic carbohydrates such as trehalose ester and glabrigenin disclosed in the present invention can also be used, and any single agent of the acid agent, the formic acid-containing agent and the non-acidic carbohydrate can be arbitrarily combined. After the breeding test on fragrant piglets, the present invention found that after mixing fumaric acid with the adjuvant and using it in the feed, the breeding growth effect of fragrant piglets can be improved, and the growth-promoting performance of fragrant piglets is better. Through the breeding of different feeds, if fumaric acid is not used, and the same amount of trehalose ester used in Example 9 or the same amount used in Example 11 is used As for glabrigenin, the feed prepared by using alginate ester or glabrigenin in the above-mentioned usage amount has basically no growth-promoting effect on the breeding of fragrant piglets. When the alginate ester or glabrigenin in the above-mentioned usage amount is used in combination with fumaric acid, the effect is better than the single use of fumaric acid. However, it should be pointed out that the effect of using glabrigenin and fumaric acid in combination is weaker than that of using alginate ester and fumaric acid in combination. The growth-promoting effect of using glabrigenin and fumaric acid in combination on fragrant piglets is relatively small. When fumaric acid, trehalose ester or glabrigenin are further used together, the obtained feed has a better growth-promoting effect on fragrant piglets.

[0140] The acidulant complexes of the present invention can be used as a fortifier and added to feed, and have excellent growth-promoting effects on fragrant piglets.

[0141] 4. Urea nitrogen content test

[0142] The urea nitrogen index in the serum of the fragrant pigs after the breeding experiment was tested using a urea nitrogen kit. The test results are as follows: Figure 3 As shown, among which SL1-11 refers to the growth promotion test. The fortified feeds in the present invention have different effects on reducing urea nitrogen in the serum of fragrant piglets. The completion of the fortified feed in the present invention requires the addition of a fortifier, and the fortifier must contain fumaric acid. After using fumaric acid, other adjuvants with a usage less than fumaric acid can also be added. The adjuvants can use one or more of the acidic agents such as formic acid, benzoic acid, citric acid, phosphoric acid and lactic acid, and one or more of the formic acid-containing agents such as formic acid-ammonium formate, succinic acid and potassium diformate can also be used. One or two of the non-acidic carbohydrates such as trehalose esters and liquiritigenin disclosed in the present invention can also be used, and any single agent of the acidic agent, the formic acid-containing agent and the non-acidic carbohydrate agent can be arbitrarily combined. After the breeding test of fragrant piglets, the present invention found that after mixing fumaric acid with an adjuvant and using it in feed, the blood of fragrant piglets can be reduced. The effect of reducing urea nitrogen in the serum of fragrant piglets was studied by breeding with different feeds. If fumaric acid is not used, but trehalose esters are used in the same amount as in Example 9 or glabrigenin is used in the same amount as in Example 11, the feed prepared by the above-mentioned amount of trehalose esters or glabrigenin has basically no effect on reducing urea nitrogen in the serum of fragrant piglets. When the above-mentioned amount of trehalose esters or glabrigenin is used in combination with fumaric acid, the effect of its use is better than the single use of fumaric acid. However, it should be pointed out that the effect of glabrigenin and fumaric acid in combination is weaker than the effect of trehalose esters and fumaric acid in combination. The effect of glabrigenin and fumaric acid in combination on reducing urea nitrogen in the serum of fragrant piglets is relatively small. When fumaric acid, trehalose esters or glabrigenin are further used together, the obtained feed has a better effect on reducing urea nitrogen in the serum of fragrant piglets.

[0143] The embodiments and / or implementation methods described above are only used to illustrate the preferred embodiments and / or implementation methods for realizing the technology of the present invention, and are not intended to limit the implementation methods of the technology of the present invention in any form. Any technical personnel in this field may make slight changes or modifications to other equivalent embodiments without departing from the scope of the technical means disclosed in the content of the present invention, but they should still be regarded as technologies or embodiments that are essentially the same as the present invention.

[0144] This article uses specific examples to illustrate the principles and implementation methods of this application. The description of the above embodiments is only used to help understand the method and its core ideas of this application. The above is only the preferred implementation method of this application. It should be pointed out that due to the limitations of textual expression and the objective existence of infinite specific structures, ordinary technicians in this technical field can make several improvements, modifications or changes without departing from the principles of this application, and can also combine the above technical features in an appropriate manner; these improvements, modifications, changes or combinations, or the direct application of the concept and technical solution of the invention to other occasions without improvement, should be regarded as the scope of protection of this application.

Claims

1. An acidulant complex comprising: Fumaric acid, trehalose ester and lactic acid; or, fumaric acid, trehalose ester and lactic acid, citric acid; or, fumaric acid, trehalose ester and lactic acid, citric acid, potassium diformate; The content of fumaric acid is above 50wt%, and the trehalose ester has a fructose acid group; in the preparation of the trehalose ester, fructose acid and trehalose react in anhydrous pyridine containing TBTU to prepare the trehalose ester.

2. An acidulant compound according to claim 1, characterized in that: The content of the fumaric acid is less than 96wt%.

3. An acidulant compound according to claim 1, characterized in that: The content of the fumaric acid is any one of 55wt%, 60wt%, 65wt%, 70wt%, 75wt%, 80wt%, 85wt% and 90wt%.

4. The acidulant compound according to claim 1, characterized in that: The usage amount of the trehalose is 100-400wt% of the fructose acid.

5. A feed comprising: The acidulant complex according to claim 1.

6. A feed according to claim 5, characterized in that: The feed is livestock and poultry breeding feed.

7. A feed according to claim 5, characterized in that: The feed is pig breeding feed.

8. A feed according to claim 5, characterized in that: Or, the feed is chicken breeding feed.

9. A feed according to claim 5, characterized in that: The usage amount of the acidifier complex is 0.025-3wt% of the feed.

10. Use of the acidulant complex according to any one of claims 1 to 4 in the preparation of an anti-Escherichia coli product.

11. Use of the acidulant complex according to any one of claims 1 to 4 in the preparation of a product for inhibiting Staphylococcus aureus.

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